Control valve structure, method of using same, microfluidic chip, and nucleic acid extraction device
By designing a combination of limiting holes and through holes in the control valve structure of the microfluidic chip, and utilizing the movement of the valve core and elastic membrane, the leakage problem caused by valve core displacement was solved, thus achieving stable flow of sample liquid and stability of the control valve.
Patent Information
- Application Number
- CN202180002188.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2021-08-17
- Publication Date
- 2025-12-16
- Estimated Expiration
- 2041-08-17
AI Technical Summary
The control valves of existing microfluidic chips are prone to valve core displacement when closed, leading to leakage problems.
A control valve structure was designed. By setting limiting holes and through holes between the cover plate layer and the channel layer, the valve core and the elastic membrane are used to form a sample liquid flow and blocking channel, ensuring the stable movement of the valve core within the limiting holes and avoiding displacement.
This effectively prevents leakage, ensures the normal flow of sample liquid and the stability of the control valve, and improves the sealing effect of the control valve.
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Figure CN116209850B_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present disclosure relates to the field of microfluidic technology, in particular to a control valve structure, a method for using the same, a microfluidic chip and a nucleic acid extraction device. BACKGROUND
[0002] The term microfluidic chip originated from the concept of micro total analysis system (μTAS) proposed by Manz and Widmer in the 1990s. Professor Manz successfully applied MEMS technology to the field of analytical chemistry, and soon after realized high-speed capillary electrophoresis on a microchip, which was published in journals such as Science. Since then, this field has rapidly gained attention from the academic community and has become one of the most cutting-edge technology fields in the world. Lab on a chip and microfluidic chip are different names proposed by people in this field, and as the application of this discipline expands from the initial analytical chemistry to multiple research and application fields, and researchers gain a deeper understanding of this discipline, microfluidic chip has become a general term for this field. SUMMARY
[0003] The control valve structure, the method for using the same, the microfluidic chip and the nucleic acid extraction device provided by the embodiments of the present disclosure have the following specific solutions:
[0004] In one aspect, the present disclosure provides a control valve structure, comprising:
[0005] a cover plate layer comprising at least one first limiting hole;
[0006] a first channel layer opposite to the cover plate layer, the first channel layer having a second limiting hole at a position corresponding to the first limiting hole;
[0007] a first adhesive layer located on a side of the first channel layer away from the cover plate layer, the first adhesive layer having a first through hole at a position corresponding to the second limiting hole, the first through hole being in communication with the corresponding second limiting hole;
[0008] a second channel layer located on a side of the first adhesive layer away from the first channel layer, the second channel layer comprising a liquid outlet channel in communication with all the first through holes;
[0009] at least one valve core moving in a space defined by the corresponding first limiting hole and the second limiting hole;
[0010] At least one elastic film is arranged on each side of the valve core close to the second limiting hole; when at least part of the valve core is located in the first limiting hole, the elastic film seals the first limiting hole, so that the second limiting hole, the first through hole and the liquid outlet channel form a sample liquid flow channel; when at least part of the valve core is located in the second limiting hole, the elastic film seals the second limiting hole to block the sample liquid flow channel.
[0011] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, the area of the orthogonal projection of the first limiting hole on the plane where the first channel layer is located is substantially the same as the area of the orthogonal projection of the second limiting hole on the plane where the first channel layer is located.
[0012] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, the area of the orthogonal projection of the valve core on the plane where the first channel layer is located is substantially the same as the area of the orthogonal projection of the second limiting hole on the plane where the first channel layer is located.
[0013] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, the area of the orthogonal projection of the first through hole on the plane where the first channel layer is located is smaller than the area of the orthogonal projection of the second limiting hole on the plane where the first channel layer is located.
[0014] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, the cover plate layer further comprises a first sample inlet, and the first sample inlet is located on the same side of the region where all the first limiting holes are located.
[0015] The first channel layer further comprises a second through hole in communication with the first sample inlet, and a first liquid inlet channel in communication with the second through hole and the second limiting hole.
[0016] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, the cover plate layer further comprises a second sample inlet, and the second sample inlet is located on the opposite side of the region where all the first limiting holes are located relative to the first sample inlet.
[0017] The first channel layer further comprises a third through hole in communication with the second sample inlet.
[0018] The first adhesive layer further comprises a fourth through hole in communication with the third through hole, and a fifth through hole located between the region where the fourth through hole is located and the region where all the first through holes are located, wherein the fifth through hole and the adjacent first through hole are covered by the same second limiting hole.
[0019] The second channel layer further comprises a second liquid inlet channel in communication with the fourth through hole and the fifth through hole.
[0020] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, the distance between the fifth through hole and the adjacent first through hole is 1-3 mm.
[0021] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, the fifth through hole and the first through hole are cylindrical holes, and the diameter of the fifth through hole and the first through hole is 0.5-2 mm.
[0022] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, the first adhesive layer comprises a lower adhesive layer, a release film and an upper adhesive layer arranged in a stack, the lower adhesive layer is in contact with the second channel layer, the upper adhesive layer is in contact with the first channel layer, the lower adhesive layer comprises a first through hole, a second through hole and a third through hole, the release film comprises a fourth through hole, a fifth through hole and a sixth through hole, the upper adhesive layer comprises a seventh through hole, an eighth through hole and a ninth through hole, the first through hole, the fourth through hole and the seventh through hole are mutually penetrable to form the first through hole, the second through hole, the fifth through hole and the eighth through hole are mutually penetrable to form the fourth through hole, and the third through hole, the sixth through hole and the ninth through hole are mutually penetrable to form the fifth through hole.
[0023] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, the release film further comprises a tenth through hole, the lower adhesive layer further comprises an eleventh through hole, the tenth through hole and the eleventh through hole are mutually penetrable, and the orthogonal projection of the tenth through hole on the plane where the first channel layer is located and the orthogonal projection of the eleventh through hole on the plane where the first channel layer is located are substantially coincident with the orthogonal projection of the second limiting hole on the plane where the first channel layer is located.
[0024] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, further comprising a second adhesive layer for bonding the cover layer and the first channel layer, the second adhesive layer comprises a twelfth through hole for connecting the first sample inlet and the second through hole, a thirteenth through hole for connecting the second sample inlet and the third through hole, and a receiving hole for accommodating the elastic membrane.
[0025] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, in the vertical direction of the plane where the first channel layer is located, the thickness of the second adhesive layer is substantially equal to the thickness of the elastic membrane.
[0026] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, the cover layer comprises a groove surrounding the first limiting hole, and the orthogonal projection of the groove on the plane where the first channel layer is located and the orthogonal projection of the elastic membrane on the plane where the first channel layer is located are mutually overlapped.
[0027] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, the groove comprises a first groove and / or a second groove, wherein the first groove is located on the side of the cover layer facing away from the first channel layer, the second groove is located on the side of the cover layer facing the first channel layer, the area of the first groove in the orthogonal projection on the plane of the first channel layer is substantially the same as the area of the elastic membrane in the orthogonal projection on the plane of the first channel layer, and the orthogonal projection of the second groove on the plane of the first channel layer is located within the orthogonal projection of the elastic membrane on the plane of the first channel layer.
[0028] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, the distance between the boundary of the orthogonal projection of the second groove on the plane of the first channel layer and the boundary of the orthogonal projection of the elastic membrane on the plane of the first channel layer is 0.5-1.0 mm.
[0029] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, in the vertical direction of the plane of the first channel layer, the depth of the first groove is 0.8-1.2 mm, and the depth of the second groove is 10-50 μm.
[0030] In some embodiments, in the control valve structure provided by the embodiments of the present disclosure, a protective film is further arranged on the side of the cover layer facing away from the first channel layer, the protective film is arranged corresponding to the second groove, and the orthogonal projection of the second groove on the plane of the first channel layer is located within the orthogonal projection of the protective film on the plane of the first channel layer.
[0031] On the other hand, the embodiments of the present disclosure provide a use method of the control valve structure, comprising:
[0032] At least part of the valve core is located in the first limiting hole, so that the elastic membrane seals the first limiting hole, and the sample liquid is injected into the outlet channel from the first through hole through the second limiting hole;
[0033] At least part of the valve core is located in the second limiting hole, so that the elastic membrane seals the second limiting hole, and the sample liquid is prevented from being injected into the outlet channel through the first through hole from the second limiting hole.
[0034] In some embodiments, in the use method provided by the embodiments of the present disclosure, when at least part of the valve core is located in the first limiting hole, the method further comprises: injecting sample liquid into the second limiting hole through the first sample inlet, the second through hole and the first liquid inlet channel arranged in communication.
[0035] In some embodiments, in the above use method provided by the embodiments of the present disclosure, while at least part of the valve core is located in the first limiting hole, the method further comprises:
[0036] The second sample inlet, the third through hole, the fourth through hole, the second liquid inlet channel and the fifth through hole are in communication to inject sample liquid into the second limiting hole.
[0037] In another aspect, the embodiments of the present disclosure provide a microfluidic chip comprising the above control valve structure provided by the embodiments of the present disclosure.
[0038] In another aspect, the embodiments of the present disclosure provide a nucleic acid extraction device comprising a microfluidic chip and an electromagnet, wherein the microfluidic chip is the above microfluidic chip provided by the embodiments of the present disclosure, and the electromagnet is located on the side of the second channel layer away from the cover layer. BRIEF DESCRIPTION OF DRAWINGS
[0039] Figure 1 FIG. 1 is a schematic diagram of a control valve structure in an open state in the related art;
[0040] Figure 2 FIG. 2 is a schematic diagram of a control valve structure in a closed state in the related art;
[0041] Figure 3 FIG. 3 is a schematic diagram of a stacked structure of a control valve structure provided by the embodiments of the present disclosure;
[0042] Figure 4 FIG. 4 is a schematic diagram of a control valve structure provided by the embodiments of the present disclosure in an open state;
[0043] Figure 5 FIG. 5 is a schematic diagram of a control valve structure provided by the embodiments of the present disclosure in a closed state;
[0044] Figure 6 FIG. 6 is another schematic diagram of a control valve structure provided by the embodiments of the present disclosure in an open state;
[0045] Figure 7 FIG. 7 is another schematic diagram of a control valve structure provided by the embodiments of the present disclosure in a closed state;
[0046] Figure 8 FIG. 8 is a flowchart of a use method of a control valve structure provided by the embodiments of the present disclosure;
[0047] Figure 9 FIG. 9 is a structural schematic diagram of a nucleic acid extraction device provided by the embodiments of the present disclosure. DETAILED DESCRIPTION
[0048] In order to make the purposes, technical solutions, and advantages of the embodiments of the present disclosure clearer, the technical solutions of the embodiments of the present disclosure will be described clearly and completely below with reference to the drawings of the embodiments of the present disclosure. It should be noted that the sizes and shapes of the figures in the drawings do not reflect the true proportions, but only serve to illustrate the present disclosure. And the same or similar reference signs represent the same or similar elements or elements with the same or similar functions throughout.
[0049] Unless otherwise defined, technical terms or scientific terms used herein should be understood as having the same meaning as commonly understood by one of ordinary skill in the art to which the present disclosure belongs. The terms "first", "second", and similar terms used in the specification and claims of the present disclosure do not denote any order, quantity, or importance, but are used to distinguish different components. The terms "include", "contain", and similar terms mean that the elements or objects before the terms encompass the elements or objects listed after the terms and their equivalents, and do not exclude other elements or objects. "In", "out", "up", "down", and the like are used only to represent relative positional relationships, and when the absolute positions of the described objects change, the relative positional relationships may also change accordingly.
[0050] Figure 1 And Figure 2 A schematic diagram of a control valve structure in the related art is shown, which specifically includes a channel plate 1, a cover plate 2, an iron block 3, and an elastic protective film 4, wherein the cover plate 2 has a receiving groove 01 accommodating the iron block 3. When the control valve is opened, the iron block 3 is not subjected to magnetic force, the iron block 3 is completely located in the receiving groove 01, and the sample liquid can pass through without obstruction, as shown in Figure 1 When the control valve is closed, the iron block 3 is subjected to downward magnetic force, the iron block 3 in the receiving groove 01 is adsorbed, and the elastic protective film 4 is pressed to bend and deform downward to block the sample liquid, as shown in Figure 2 However, the iron block is prone to displacement during the process of adsorbing the iron block 3, causing the control valve to close loosely and resulting in liquid leakage.
[0051] In order to at least solve the above technical problems existing in the related art, the present disclosure provides a control valve structure, as shown in Figures 3 to 5 which includes:
[0052] The cover plate layer 101 includes at least one first limiting hole 1011;
[0053] The first channel layer 102 is arranged opposite to the cover plate layer 101, and the first channel layer 102 has a second limiting hole 1021 at a position corresponding to the first limiting hole 1011;
[0054] The first adhesive layer 103 is located on the side of the first channel layer 102 away from the cover layer 101, and the first adhesive layer 103 has a first through hole 1031 at a position corresponding to the second limiting hole 1021, and the first through hole 1031 is in communication with the corresponding second limiting hole 1021;
[0055] The second channel layer 104 is located on the side of the first adhesive layer 103 away from the first channel layer 102, and the second channel layer 104 includes a liquid outlet channel 1041 in communication with all the first through holes 1031;
[0056] The at least one valve core 105 moves in the space defined by the corresponding first limiting hole 1011 and the second limiting hole 1021;
[0057] The at least one elastic film 106 is located on the side of each valve core 105 close to the second limiting hole 1021; when at least part of the valve core 105 (i.e., part or all of the valve core 105) is located in the first limiting hole 1011, the elastic film 106 seals the first limiting hole 1011, so that the second limiting hole 1021, the first through hole 1031 and the liquid outlet channel 1041 form a sample liquid flow channel; when at least part of the valve core 105 (i.e., part or all of the valve core 105) is located in the second limiting hole 1021, the elastic film 106 seals the second limiting hole 1021 to block the sample liquid flow channel.
[0058] In the control valve structure provided in the embodiments of the present disclosure, the movable space of the valve core 105 is defined by the first limiting hole 1011 of the cover layer 101 and the second limiting hole 1021 of the first channel layer 102, and the first through hole 1031 and the liquid outlet channel 1041 in communication with the second limiting hole 1021 below the second limiting hole 1021 constitute a sample liquid flow channel, so that the inlet and outlet of the sample liquid flow channel and the valve control space are not in the same layer, so that when the control valve is closed, at least part of the valve core 105 (i.e., part or all of the valve core 105) is located in the second limiting hole 1021 without displacement, the elastic film 106 seals the second limiting hole 1021 to block the sample liquid flow channel, thereby greatly improving the liquid leakage problem; in addition, when the control valve is opened, part or all of the valve core 105 is located in the first limiting hole 1011, the elastic film 106 seals the first limiting hole 1011, and the second limiting hole 1021, the first through hole 1031 and the liquid outlet channel 1041 form a sample liquid flow channel, ensuring the normal flow of the sample liquid.
[0059] It should be noted that, in the present disclosure, in order to make the valve core 105 have a smaller pressure on the elastic film 106, so as to facilitate the normal reset of the elastic film 106, a smaller and lighter valve core 105, such as a steel column, can be selected. Moreover, since the smaller and lighter valve core 105 will not cause the elastic film 106 to deform, when the control valve is opened, the valve core 105 can be completely located in the first limiting hole 1011, or can be partially located in the first limiting hole 1011 (that is, the valve core 105 will protrude out of the first limiting hole 1011). In addition, when the control valve is closed, the valve core 105 presses down the elastic film 106, as long as the elastic film 106 can seal the second limiting hole 1021, therefore, the valve core 105 can be partially located in the second limiting hole 1021 (as shown in FIG. 13B), or can be completely located in the second limiting hole 1021. Optionally, in the vertical direction of the plane where the first channel layer 102 is located, the height of the valve core 105 can be 1mm-3mm. Figure 5
[0060] In some embodiments, in the above control valve structure provided by the embodiments of the present disclosure, in order to ensure the limiting effect of the first limiting hole 1011 and the second limiting hole 1021 on the valve core 105, as shown in FIG. 13A, the area of the orthogonal projection of the first limiting hole 1011 on the plane where the first channel layer 102 is located can be substantially the same as the area of the orthogonal projection of the second limiting hole 1021 on the plane where the first channel layer 102 is located. Figures 3 to 5
[0061] It should be noted that, in the above control valve structure provided by the embodiments of the present disclosure, due to the limitation of process conditions or the influence of other factors such as measurement, “substantially” may be completely equivalent, or there may be some deviation, therefore, as long as the “substantially” relationship between the features meets the error (for example, 10% floating up and down) allowance, it belongs to the protection scope of the present disclosure.
[0062] In some embodiments, in the above control valve structure provided by the embodiments of the present disclosure, as shown in FIG. 13B, the area of the orthogonal projection of the valve core 105 on the plane where the first channel layer 102 is located can be substantially the same as the area of the orthogonal projection of the second limiting hole 1021 on the plane where the first channel layer 102 is located. Figures 3 to 5
[0063] In some embodiments, the valve core 105 can be a steel column, and the first limiting hole 1011 and the second limiting hole 1021 can be cylindrical holes, at this time, the diameter of the valve core 105, the first limiting hole 1011 and the second limiting hole 1021 can be 5mm-8mm.
[0064] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, as shown in Figures 3 to 5 , the area of the orthogonal projection of the first through hole 1031 of the first adhesive layer 103 on the plane where the first channel layer 102 is located is smaller than the area of the orthogonal projection of the second limiting hole 1021 on the plane where the first channel layer 102 is located. In this way, when the valve core 105 is pressed to the second limiting hole 1021 under the magnetic force, the deformed elastic membrane 106 can be supported by the first adhesive layer 103, so that the elastic membrane 106 can effectively block the sample liquid flow channel, thereby improving the liquid leakage prevention effect.
[0065] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, as shown in Figures 3 to 5 , the cover plate layer 101 can further include a first sample inlet 1012, and the first sample inlet 1012 is located on the same side of the region where all the first limiting holes 1011 are located. In some embodiments, the position of the first sample inlet 1012 can be selected according to the size of the space around the region where the first limiting hole 1011 is located in the cover plate layer 101. For example, as shown in Figure 3 , when the spaces on both sides of the first limiting hole 1011 in the X direction are large and the spaces on both sides of the first limiting hole 1011 in the Y direction are small, the first sample inlet 1012 can be arranged on one side (for example, the right side as shown in Figure 3 ) of the first limiting hole 1011 in the X direction for the convenience of processing.
[0066] The first channel layer 102 can further include a second through hole 1022 in communication with the first sample inlet 1012, and a first liquid inlet channel 1023 in communication with the second through hole 1022 and the second limiting hole 1021.
[0067] In specific implementation, after the sample liquid is injected from the first sample inlet 1012, the sample liquid flows through the second through hole 1022 and the first liquid inlet channel 1023 in sequence, and then reaches the second limiting hole 1021. When the control valve is opened, at least part of the valve core 105 is located in the first limiting hole 1011, the elastic membrane 106 seals the first limiting hole 1011, and the sample liquid at the second limiting hole 1021 flows out after being injected into the liquid outlet channel 1041 through the first through hole 1031. When the control valve is closed, at least part of the valve core 105 is located in the second limiting hole 1021, the elastic membrane 106 seals the second limiting hole 1021, and the sample liquid at the second limiting hole 1021 cannot flow. In this case, the control valve is an upper inlet and lower outlet type valve.
[0068] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, as shown in Figure 3 , Figure 6 and Figure 7As shown, the cover layer 101 can further include a second sample inlet 1013, which is located on the opposite side of the first sample inlet 1012 relative to the region where all the first limiting holes 1011 are located.
[0069] The first channel layer 102 can further include a third through hole 1024 in communication with the second sample inlet 1013.
[0070] The first adhesive layer 103 can further include a fourth through hole 1032 in communication with the third through hole 1024, and a fifth through hole 1033 located between the region where the fourth through hole 1032 is located and the region where all the first through holes 1031 are located, wherein the fifth through hole 1033 and the adjacent first through hole 1031 are covered by the same second limiting hole 1021.
[0071] The second channel layer 104 can further include a second liquid inlet channel 1042 in communication with the fourth through hole 1032 and the fifth through hole 1033.
[0072] In a specific implementation, after the sample liquid is injected from the second sample inlet 1013, it flows through the third through hole 1024, the fourth through hole 1032, the second liquid inlet channel 1042, and the fifth through hole 1033 in sequence, and then reaches the second limiting hole 1021. When the control valve is open, at least part of the valve core 105 is located in the first limiting hole 1011, and the elastic membrane 106 seals the first limiting hole 1011. The sample liquid at the second limiting hole 1021 flows out after being injected into the liquid outlet channel 1041 through the first through hole 1031. When the control valve is closed, at least part of the valve core 105 is located in the second limiting hole 1021, and the elastic membrane 106 seals the second limiting hole 1021. The sample liquid at the second limiting hole 1021 cannot flow. In this case, the control valve is a down-in and down-out type valve.
[0073] In some embodiments, in the above control valve structure provided by the embodiments of the present disclosure, the distance between the fifth through hole 1033 and the adjacent first through hole 1031 can be 1 mm-3 mm, so as to ensure that the fifth through hole 1033 and the adjacent first through hole 1031 can be covered by the same second limiting hole 1021, thereby achieving the flow of the sample liquid from the fifth through hole 1033 and the flow of the sample liquid from the adjacent first through hole 1031, as shown in Figure 6 .
[0074] In some embodiments, in the above control valve structure provided by the embodiments of the present disclosure, the fifth through hole 1033 and the first through hole 1031 are cylindrical holes, and the diameter of the fifth through hole 1033 and the first through hole 1031 can be 0.5 mm-2 mm, so as to ensure that the fifth through hole 1033 and the adjacent first through hole 1031 can be covered by the same second limiting hole 1021, thereby achieving the flow of the sample liquid from the fifth through hole 1033 and the flow of the sample liquid from the adjacent first through hole 1031, as shown in Figure 6As shown.
[0075] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, as shown in Figure 3 The first adhesive layer 103 can include a lower adhesive layer a, a release film b and an upper adhesive layer c arranged in layers, wherein the lower adhesive layer a is in contact with the second channel layer 104, the upper adhesive layer c is in contact with the first channel layer 102, the lower adhesive layer a includes a first via a1, a second via a2 and a third via a3, the release film b includes a fourth via b1, a fifth via b2 and a sixth via b3, and the upper adhesive layer c includes a seventh via c1, an eighth via c2 and a ninth via c3, wherein the first via a1, the fourth via b1 and the seventh via c1 pass through each other to form a first through-hole 1031, the second via a2, the fifth via b2 and the eighth via c2 pass through each other to form a fourth through-hole 1032, and the third via a3, the sixth via b3 and the ninth via c3 pass through each other to form a fifth through-hole 1033.
[0076] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, as shown in Figure 3 The release film b can further include a tenth via b4, and the lower adhesive layer a can further include an eleventh via a4, the tenth via b4 and the eleventh via a4 pass through each other, and the tenth via b4 and the eleventh via a4 are substantially coincident with the projection of the second limiting hole 1021 on the plane of the first channel layer 102. The above arrangement makes the first adhesive layer 103 directly below the second limiting hole 1021 only include the upper adhesive layer c, which has a smaller thickness and can reduce the flow resistance of the sample liquid to the first adhesive layer 103.
[0077] Optionally, the first adhesive layer 103 can be processed using a conventional double-sided adhesive tape, which includes an upper release film, an adhesive layer, and a lower release film. In a specific implementation, the first adhesive layer 103 can be prepared using two layers of conventional double-sided adhesive tapes, which are referred to as a first layer of conventional double-sided adhesive tape and a second layer of conventional double-sided adhesive tape. Specifically, the first layer of conventional double-sided adhesive tape can be semi-transversely cut at the positions of the first limiting holes 1011 (i.e., only the upper release film and the adhesive layer are cut, and the lower release film is retained), and the first layer of conventional double-sided adhesive tape can be fully cut at the positions of the first through hole 1031, the fourth through hole 1033, and the fifth through hole 1033 (i.e., the upper release film, the adhesive layer, and the lower release film are completely cut off). The second layer of conventional double-sided adhesive tape can be fully cut at the positions of the first through hole 1031, the fourth through hole 1033, and the fifth through hole 1033. In use, the upper release film of the second layer of conventional double-sided adhesive tape is peeled off and attached to the lower side of the first layer of conventional double-sided adhesive tape, the upper release film of the first layer of conventional double-sided adhesive tape is then peeled off and attached to the lower side of the first channel layer 104, and the lower release film of the second layer of conventional double-sided adhesive tape is then peeled off to allow the second channel layer 104 to be attached thereto. In this way, the adhesive layer of the first layer of conventional double-sided adhesive tape, the lower release film of the first layer of conventional double-sided adhesive tape, and the adhesive layer of the second layer of conventional double-sided adhesive tape together constitute the first adhesive layer 103.
[0078] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, as shown in Figure 3 the second adhesive layer 107 for bonding the cover layer 101 and the first channel layer 102 can further include a twelfth via hole 1071 for communicating the first sample inlet 1012 and the second through hole 1022, a thirteenth via hole 1072 for communicating the second sample inlet 1013 and the third through hole 1024, and a receiving hole 1073 for accommodating the elastic film 106. The receiving hole 1073 can ensure that the valve core 105 moves freely in the space defined by the first limiting hole 1011 and the second limiting hole 1021, the twelfth via hole 1071 can ensure that the sample liquid injected from the first sample inlet 1012 flows smoothly into the second limiting hole 1021, and the thirteenth via hole 1072 can ensure that the sample liquid injected from the second sample inlet 1013 flows smoothly into the second limiting hole 1021.
[0079] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, the thickness of the second adhesive layer 107 is substantially equal to the thickness of the elastic film 106 in the vertical direction of the plane in which the first channel layer 102 is located. In this way, there is almost no height difference between the second adhesive layer 107 and the elastic film 106, thereby ensuring that there is no gap between the second adhesive layer 107 and the elastic film 106, and avoiding liquid leakage.
[0080] In addition, it is worth noting that, in order to facilitate processing, facilitate mass production, and improve the yield, the thickness uniformity of each layer of the cover layer 101, the first channel layer 102, the first adhesive layer 103, and the second channel layer 104 is good.
[0081] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, as shown in Figure 3 the cover plate layer 101 can include a groove 1014 around the first limiting hole 1011, and a projection of the groove 1014 on a plane where the first channel layer 102 is located and a projection of the elastic film 106 on the plane where the first channel layer 102 is located overlap with each other. The existence of the groove 1014 can increase the gas space around the valve core 105, reduce the change of the air pressure around the valve core 105, and facilitate to improve the stability of the control valve.
[0082] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, in order to increase the gas space around the valve core 105 as much as possible to further improve the stability of the control valve, the groove 1014 can include a first groove and / or a second groove, wherein the first groove is located on a side of the cover plate layer 101 away from the first channel layer 102, the second groove is located on a side of the cover plate layer 101 facing the first channel layer 102, an area of a projection of the first groove on a plane where the first channel layer 102 is located is substantially the same as an area of a projection of the elastic film 106 on the plane where the first channel layer 102 is located, and a projection of the second groove on the plane where the first channel layer 102 is located is located within a projection of the elastic film 106 on the plane where the first channel layer 102 is located.
[0083] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, a distance between a boundary of a projection of the second groove on a plane where the first channel layer 102 is located and a boundary of a projection of the elastic film 106 on the plane where the first channel layer 102 is located can be 0.5mm-1.0mm, so as to ensure the edge pressing effect of the elastic film 106 on the second groove.
[0084] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, in order to improve the stability effect of the control valve, in a vertical direction of the plane where the first channel layer 102 is located, a depth of the first groove can be 0.8mm-1.2mm, and a depth of the second groove can be 10μm-50μm.
[0085] In some embodiments, in the control valve structure provided in the embodiments of the present disclosure, as shown in Figures 4 to 7 the cover plate layer 101 can further include a protective film 108 located on a side of the cover plate layer 101 away from the first channel layer 102, the protective film 108 is arranged corresponding to the second groove, and a projection of the second groove on a plane where the first channel layer 102 is located is located within a projection of the corresponding protective film 108 on the plane where the first channel layer 102 is located, so that the second groove and the first limiting hole 1011 surrounded by the second groove can be sealed by the protective film 108, and the valve core 105 can be prevented from falling off from the first limiting hole 1011.
[0086] Based on the same inventive concept, this disclosure provides a method for using the above-described control valve structure. Since the principle of this method for solving the problem is similar to that of the control valve structure, the implementation of the method provided in this disclosure can refer to the implementation of the control valve structure provided in this disclosure. Repeated details will not be repeated.
[0087] Specifically, this disclosure provides a method for using the above-described control valve structure, such as... Figure 8 As shown, the following steps may be included:
[0088] S801, at least a portion of the control valve core is located in the first limiting hole, so that the elastic membrane seals the first limiting hole, and the sample liquid is injected into the outlet channel from the second limiting hole through the first through hole;
[0089] S802, at least a portion of the control valve core is located within the second limiting hole, such that the elastic membrane seals the second limiting hole, preventing the sample liquid from being injected into the outlet channel from the second limiting hole through the first through hole.
[0090] In some embodiments, in the above-described method of use provided in the present disclosure, while performing step S801 and controlling at least a portion of the valve core to be located within the first limiting hole, the following steps can also be performed: injecting sample liquid into the second limiting hole through the first sample inlet, the second through hole and the first liquid inlet channel that are connected.
[0091] In some embodiments, in the above-described method of use provided in this disclosure, while performing step S801 and controlling at least a portion of the valve core to be located within the first limiting hole, the following steps may also be performed:
[0092] Sample liquid is injected into the second limiting hole through the connected second inlet, third through hole, fourth through hole, second liquid inlet channel and fifth through hole.
[0093] Based on the same inventive concept, this disclosure provides a microfluidic chip, including the control valve structure described above. Since the principle by which this microfluidic chip solves the problem is similar to that of the control valve structure described above, the implementation of the microfluidic chip provided in this disclosure can refer to the implementation of the control valve structure described above, and repeated details will not be elaborated further.
[0094] On the other hand, embodiments of this disclosure provide a nucleic acid extraction device, such as... Figure 9 As shown, it includes a microfluidic chip 001 and an electromagnet 002, wherein the microfluidic chip 001 is the microfluidic chip provided in the embodiments of this disclosure, and the electromagnet 002 is located on the side of the second channel layer 104 away from the cover plate layer 101.
[0095] In some embodiments, an electromagnet 002 can be arranged below each valve core 105 to achieve independent control of each valve core 105 through the corresponding electromagnet 002. Specifically, the electromagnet 002 can be controlled to be forward energized to limit the valve core 105 in the second limiting hole 1021, and by controlling the electromagnet 002 to be de-energized or reverse energized, the valve core 105 is limited in the first limiting hole 1011.
[0096] Obviously, those skilled in the art can make various modifications and variations to the embodiments of the present disclosure without departing from the spirit and scope of the embodiments of the present disclosure. Thus, if these modifications and variations of the embodiments of the present disclosure fall within the scope of the claims of the present disclosure and their equivalent technologies, the present disclosure also intends to include these modifications and variations.
Claims
1. A control valve structure, wherein, The application relates to a liquid sample valve, comprising: a cover layer comprising at least one first limiting hole; a first channel layer opposite to the cover layer, the first channel layer having a second limiting hole at a position corresponding to the first limiting hole; a first adhesive layer on a side of the first channel layer away from the cover layer, the first adhesive layer having a first through hole at a position corresponding to the second limiting hole, the first through hole being in communication with the corresponding second limiting hole; a second channel layer on a side of the first adhesive layer away from the first channel layer, the second channel layer comprising a liquid outlet channel in communication with all the first through holes; at least one valve core moving in a space defined by the corresponding first limiting hole and second limiting hole; at least one elastic film on a side of each valve core close to the second limiting hole; when at least part of the valve core is located in the first limiting hole, the elastic film seals the first limiting hole, so that the second limiting hole, the first through hole and the liquid outlet channel form a sample liquid flow channel; when at least part of the valve core is located in the second limiting hole, the elastic film seals the second limiting hole to block the sample liquid flow channel. The cover layer further comprises a first sample inlet on the same side of the region where all the first limiting holes are located. The first channel layer further comprises a second through hole in communication with the first sample inlet, and a first liquid inlet channel in communication with the second through hole and the second limiting hole. The cover layer further comprises a second sample inlet on the opposite side of the region where all the first limiting holes are located relative to the first sample inlet. The first channel layer further comprises a third through hole in communication with the second sample inlet. The first adhesive layer further comprises a fourth through hole in communication with the third through hole, and a fifth through hole between the region where the fourth through hole is located and the region where all the first through holes are located, wherein the fifth through hole and the adjacent first through hole are covered by the same second limiting hole. The second channel layer further comprises a second liquid inlet channel in communication with the fourth through hole and the fifth through hole.
2. The control valve structure as recited in claim 1, wherein The first limiting hole has a projection area on the plane where the first channel layer is located, which is substantially the same as the projection area of the second limiting hole on the plane where the first channel layer is located.
3. The control valve structure as recited in claim 2, wherein The valve core has a projection area on the plane where the first channel layer is located, which is substantially the same as the projection area of the second limiting hole on the plane where the first channel layer is located.
4. The control valve structure as recited in claim 1, wherein The first through hole has a projection area on the plane where the first channel layer is located, which is smaller than the projection area of the second limiting hole on the plane where the first channel layer is located.
5. The control valve structure as recited in claim 1, wherein The distance between the fifth through hole and the adjacent first through hole is 1mm-3mm.
6. The control valve structure as claimed in claim 1, wherein The fifth through hole and the first through hole are cylindrical holes, and the diameter of the fifth through hole and the first through hole is 0.5mm-2mm.
7. The control valve structure as claimed in claim 1, wherein The first adhesive layer comprises a lower adhesive layer, a release film and an upper adhesive layer arranged in a stack, wherein the lower adhesive layer is in contact with the second channel layer, the upper adhesive layer is in contact with the first channel layer, the lower adhesive layer comprises a first via hole, a second via hole and a third via hole, the release film comprises a fourth via hole, a fifth via hole and a sixth via hole, the upper adhesive layer comprises a seventh via hole, an eighth via hole and a ninth via hole, wherein the first via hole, the fourth via hole and the seventh via hole are mutually through to form the first through hole, the second via hole, the fifth via hole and the eighth via hole are mutually through to form the fourth through hole, and the third via hole, the sixth via hole and the ninth via hole are mutually through to form the fifth through hole.
8. The control valve structure as recited in claim 7, wherein The release film further comprises a tenth via hole, and the lower adhesive layer further comprises an eleventh via hole, wherein the tenth via hole and the eleventh via hole are mutually through, and the orthogonal projection of the tenth via hole on the plane of the first channel layer and the orthogonal projection of the eleventh via hole on the plane of the first channel layer substantially coincide with the orthogonal projection of the second limiting hole on the plane of the first channel layer.
9. The control valve structure as claimed in claim 1, wherein Further comprising a second adhesive layer for bonding the cover plate layer and the first channel layer, wherein the second adhesive layer comprises a twelfth via hole for connecting the first sample inlet and the second through hole, a thirteenth via hole for connecting the second sample inlet and the third through hole, and a receiving hole for accommodating the elastic film.
10. The control valve structure as recited in claim 9, wherein In the vertical direction of the plane of the first channel layer, the thickness of the second adhesive layer is substantially equal to the thickness of the elastic film.
11. The control valve structure according to any one of claims 1 to 10, wherein The cover plate layer comprises a groove around the first limiting hole, and the orthogonal projection of the groove on the plane of the first channel layer and the orthogonal projection of the elastic film on the plane of the first channel layer are mutually overlapped.
12. The control valve structure as claimed in claim 11, wherein, The groove comprises a first groove and / or a second groove, wherein the first groove is located on the side of the cover plate layer away from the first channel layer, the second groove is located on the side of the cover plate layer facing the first channel layer, the area of the orthogonal projection of the first groove on the plane of the first channel layer is substantially the same as the area of the orthogonal projection of the elastic film on the plane of the first channel layer, and the orthogonal projection of the second groove on the plane of the first channel layer is located within the orthogonal projection of the elastic film on the plane of the first channel layer.
13. The control valve structure as claimed in claim 12, wherein, The distance between the boundary of the orthogonal projection of the second groove on the plane of the first channel layer and the boundary of the orthogonal projection of the elastic film on the plane of the first channel layer is 0.5mm-1.0mm.
14. The control valve structure as claimed in claim 12, wherein, In the vertical direction of the plane of the first channel layer, the depth of the first groove is 0.8mm-1.2mm, and the depth of the second groove is 10μm-50μm.
15. The control valve structure as claimed in claim 12, wherein Further comprising a protective film located on the side of the cover plate layer away from the first channel layer, wherein the second groove is arranged corresponding to the protective film, and the orthogonal projection of the second groove on the plane of the first channel layer is located within the orthogonal projection of the protective film on the plane of the first channel layer.
16. A method of using a control valve structure as claimed in any one of claims 1-15, wherein, Comprise: At least part of the control valve core is located in the first limiting hole, so that the elastic membrane seals the first limiting hole, and the sample liquid is injected into the outlet channel from the first through hole through the second limiting hole; At least part of the control valve core is located in the second limiting hole, so that the elastic membrane seals the second limiting hole, and the sample liquid is prevented from being injected into the outlet channel from the second limiting hole through the first through hole.
17. The method of use of claim 16, wherein, At least part of the control valve core is located in the first limiting hole, and further comprising: injecting the sample liquid into the second limiting hole through the first sample inlet, the second through hole and the first liquid inlet channel which are connected.
18. The method of use of claim 16, wherein, At least part of the control valve core is located in the first limiting hole, and further comprising: Injecting the sample liquid into the second limiting hole through the second sample inlet, the third through hole, the fourth through hole, the second liquid inlet channel and the fifth through hole which are connected.
19. A microfluidic chip, wherein, The control valve structure comprises any one of claims 1-15.
20. A nucleic acid extraction device, wherein, The microfluidic chip comprises the microfluidic chip of claim 19, and the electromagnet is located on the side of the second channel layer away from the cover layer.
Citation Information
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