Embedding Structure of Probiotics and Its Preparation Method

Abstract

The present invention provides an embedding structure of probiotics and a preparation method thereof. The embedding structure includes a core and a shell. Among them, the core includes a first core layer, a second core layer, and a third core layer. The first core layer contains postbiotics. The second core layer coats the first core layer and contains active probiotics. The third core layer coats the second core layer and contains prebiotics. The shell coats the core in a multi-layer structure and contains fiber, colloid, protein, and fatty acid. The above embedding structure of probiotics has a better protective effect on probiotics.

Description

Technical Field

[0001] The present invention relates to the field of probiotic products, and particularly to an embedding structure of probiotics and a preparation method thereof. Background Art

[0002] Probiotics are important symbiotic bacteria in the human body and have different physiological and active functions depending on the strains and species. For example, when humans or animals consume probiotics, it can improve the quality of the intestinal flora in them, so as to regulate the digestive tract flora / digestive tract function and enhance immunity, etc., thereby promoting the health of the host.

[0003] According to the current manufacturing method, probiotics mostly undergo a drying process first to facilitate subsequent preparation operations. Therefore, most current probiotic products are in powder form. However, probiotics may lose their activity during the drying process, which affects the physiological and active effects of probiotic products, and powdered probiotics are also prone to losing their activity due to poor storage conditions. In addition, powdered probiotic products are prone to dusting during the preparation or consumption process, which not only causes inconvenience during production but also easily leads to choking during consumption.

[0004] Therefore, how to protect the activity of probiotics in probiotic products is the goal that needs to be urgently pursued at present. Summary of the Invention

[0005] The present invention provides an embedding structure of probiotics and a preparation method thereof. The embedding structure forms a core by sequentially coating postbiotics, probiotics, and prebiotics, and then coats the core with fiber, colloid, protein, and fatty acid to form a shell; according to this embedding structure, the probiotics have a better number of viable bacteria.

[0006] The specific technical solutions are as follows:

[0007] The present invention provides an embedding structure of probiotics, which comprises: a core and a shell; the core comprises: a first core layer, a second core layer, and a third core layer; the first core layer comprises postbiotics; the second core layer coats the first core layer and comprises viable probiotics; the third core layer coats the second core layer and comprises prebiotics; the shell coats the core with a multi-layer structure and comprises fiber, colloid, protein, and fatty acid.

[0008] Preferably, the postbiotics comprise the fermented product of the probiotics.

[0009] Preferably, the shell comprises: a first shell layer, a second shell layer, and a third shell layer; the first shell layer coats the core; the second shell layer coats the first shell layer; the third shell layer coats the second shell layer;

[0010] The combination of raw materials contained in each shell layer is one of the following:

[0011] A1: The first shell layer contains protein and fatty acid; the second shell layer contains colloid; the third shell layer contains fiber;

[0012] A2: The first shell layer contains colloid; the second shell layer contains protein and fatty acid; the third shell layer contains fiber;

[0013] A3: The first shell layer contains protein and fatty acid; the second shell layer contains fiber; the third shell layer contains colloid.

[0014] Preferably, the probiotic bacteria include at least one of Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, Lactobacillus acidophilus, Bacillus coagulans, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus johnsonii, Lactobacillus paracasei, Lactobacillus pentosus, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactococcus lactis, Streptococcus thermophiles, Pediococcus pentosaceus, and Pediococcus acidilactici.

[0015] Further preferably, the probiotic comprises at least one of Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, and Lactobacillus acidophilus.

[0016] Preferably, the prebiotic comprises at least one of fructo-oligosaccharide (FOS), galacto-oligosaccharide (GOS), inulin, stachyose, free xylo-oligosaccharide, soy oligosaccharide, isomaltooligosaccharide, lactulose oligosaccharide, lactulose, raffinose, polydextrose, polyglucose, grape oligosaccharide, mannan oligosaccharide, arabinan oligosaccharide, fuco-oligosaccharide, chito-oligosaccharide, lactitol, and lactulose.

[0017] Preferably, in the embedding structure of the probiotic, the weight percentage content of the postbiotic is 1-5%; the weight percentage content of the probiotic is 1-5%; the weight percentage content of the prebiotic is 10-20%; the weight percentage content of the fatty acid is 1-5%, the weight percentage content of the protein is 7-15%, and the weight percentage content of the colloid is 2-5%, and the rest is fiber.

[0018] Preferably, the protein comprises at least one of skim milk powder, concentrated whey protein, casein, hydrolyzed soy protein, glycoprotein, and corn protein.

[0019] Preferably, the fatty acid comprises at least one of linseed oil powder, spirulina powder, and lecithin.

[0020] Preferably, the colloid comprises at least one of gum arabic, pectin, corn sugar gum, agar, alginate, β-glucan, locust bean gum, and carrageenan.

[0021] Preferably, the fiber comprises at least one of amylose, microcrystalline cellulose, sorbitol, erythritol, resistant dextrin, oat fiber, citrus fiber, and Jerusalem artichoke fiber.

[0022] The present invention also provides a preparation method of an embedding structure of a probiotic, comprising:

[0023] (1) Inoculating the probiotic into a culture medium for fermentation;

[0024] (2) After fermentation is completed, the probiotic cells and the fermentation supernatant are separated;

[0025] (3) After sterilizing and drying the fermentation supernatant, the powdered fermentation supernatant is introduced into a fluidized bed as the first core layer;

[0026] (4) The cells are diluted into a fluid state and atomized and sprayed into the fluidized bed, so that the cells adsorb and coat the first core layer to form a second core layer and a plurality of granular cores;

[0027] (5) A multi-layered shell is formed to coat the plurality of cores described in step (4), wherein the shell comprises fibers, colloids, proteins, and fatty acids.

[0028] Preferably, the preparation method further comprises: before forming the shell (i.e., before step (5)), spraying prebiotics into the fluidized bed in an atomized manner, so that the prebiotics coat the second core layer to form a third core layer.

[0029] Preferably, in the embedded structure of the probiotics, the weight percentage content of the fermentation supernatant is 1-5%; the weight percentage content of the probiotics is 1-5%; the weight percentage content of the prebiotics is 10-20%; the weight percentage content of the fatty acids is 1-5%, the weight percentage content of the proteins is 7-15%, and the weight percentage content of the colloids is 2-5%, and the rest is fibers.

[0030] Preferably, the step of forming the shell comprises:

[0031] (A) Spraying the first shell layer material into the fluidized bed in an atomized manner, so that the first shell layer material coats the core to form a first shell layer; the first shell layer material comprises proteins and fatty acids, or colloids;

[0032] (B) Collecting the particles containing the first shell layer, uniformly mixing them with the second shell layer material and then drying, so that the second shell layer material coats the first shell layer and forms a second shell layer; the second shell layer material comprises colloids, or proteins and fatty acids, or fibers;

[0033] (C) Uniformly mixing the particles containing the second shell layer with the third shell layer material, granulating and then drying, so that the third shell layer material coats the second shell layer and forms a third shell layer; the third shell layer material comprises fibers or colloids; and the first shell layer material, the second shell layer material, and the third shell layer material are different from each other.

[0034] Preferably, the second shell layer material is uniformly mixed with the particles containing the first shell layer by a shot blasting machine.

[0035] Preferably, the third shell layer material is uniformly mixed with the particles containing the second shell layer by a shot blasting machine and then pelletized by shot blasting.

[0036] Preferably, the combination of the materials of each shell layer is one of the following:

[0037] A1: The first shell layer material contains protein and fatty acid; the second shell layer material contains colloid; the third shell layer material contains fiber;

[0038] A2: The first shell layer material contains colloid; the second shell layer material contains protein and fatty acid; the third shell layer material contains fiber;

[0039] A3: The first shell layer material contains protein and fatty acid; the second shell layer material contains fiber; the third shell layer material contains colloid.

[0040] Preferably, the inlet air temperature of the fluidized bed is less than or equal to 60 degrees Celsius.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] (1) In the present invention, postbiotics, probiotics and prebiotics are sequentially coated to form a core, and then the core is coated with fiber, colloid, protein and fatty acid to form a shell, obtaining an embedding structure of probiotics; according to this embedding structure, the probiotics have a better viable bacteria count.

[0043] (2) The probiotics of the present invention do not need to be dried in advance, but are dried during the embedding process, so that the heat damage to the probiotics caused by the processing process can be reduced to maintain better probiotic activity. Description of the Drawings

[0044] Figure 1 It is an embedding structure of probiotics with a double-layer core layer.

[0045] Figure 2 It is an embedding structure of probiotics with a triple-layer core layer.

[0046] Figure 3 It is the test result of the probiotic activity of the probiotic embedding structure.

[0047] In the figure, 10a is the embedding structure of probiotics (double-layer core layer); 10b is the embedding structure of probiotics (triple-layer core layer); 11a is the core (double-layer core layer); 11b is the core (triple-layer core layer); 111 is the first core layer; 112 is the second core layer; 113 is the third core layer; 12 is the shell; 121 is the first shell layer; 122 is the second shell layer; 123 is the third shell layer. Detailed Embodiments

[0048] The embodiments of the present invention will be described in detail below and illustrated in conjunction with the accompanying drawings. In addition to these detailed descriptions, the present invention can also be widely implemented in other embodiments. Any simple substitution, modification, or equivalent change of the above-described embodiments is included within the scope of the invention and shall be subject to the scope of the patent application. In the description of the specification, in order to enable the reader to have a more complete understanding of the present invention, many specific details are provided; however, the present invention may still be implemented on the premise of omitting some or all of the specific details. In addition, well-known steps or elements are not described in detail to avoid unnecessary limitations on the present invention. The same or similar symbols are used in the drawings. It should be particularly noted that the drawings are only for illustrative purposes and do not represent the actual size or quantity of the elements. Some details may not be fully shown in order to simplify the drawings.

[0049] Please refer to Figure 1 , the embedding structure 10a of the probiotic in one embodiment of the present invention includes a core 11a and a shell 12. The core 11a includes a first core layer 111 and a second core layer 112. The second core layer 112 coats the first core layer 111 to form a core 11a with a double-layer structure. The main component of the first core layer 111 is postbiotics, and the second core layer 112 is an active probiotic. In one embodiment, the postbiotics of the first core layer 111 may be the fermentation product of the probiotic of the second core layer 112, but not limited thereto. For example, in another embodiment, the probiotic that ferments to produce the postbiotics of the first core layer 111 may be different from the probiotic of the second core layer 112.

[0050] In one embodiment, the probiotics in the second core layer 112 may be at least one of Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, Lactobacillus acidophilus, Bacillus coagulans, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus johnsonii, Lactobacillus paracasei, Lactobacillus pentosus, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactococcus lactis, Streptococcus thermophiles, Pediococcus pentosaceus, and Pediococcus acidilactici. In a preferred embodiment, the probiotics in the second core layer 112 may be at least one of Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, and Lactobacillus acidophilus.

[0051] Continuing the above description, the outer shell 12 may also coat the core 11 in a multi-layer structure. For example, as Figure 1As shown, the outer shell 12 includes a first shell layer 121, a second shell layer 122, and a third shell layer 123 from the inside out, that is, the first shell layer 121 wraps the core 11, the second shell layer 122 wraps the first shell layer 121, and the third shell layer 123 wraps the second shell layer 122. In an embodiment, the main components of the first shell layer 121 are protein and fatty acid, or colloid; the main components of the second shell layer 122 are colloid, protein and fatty acid, or fiber; the main components of the third shell layer 123 are fiber or colloid. It should be noted that the first shell layer 121, the second shell layer 122, and the third shell layer 123 are made of different materials. The detailed composition of the outer shell 12 will be described later.

[0052] In an embodiment, the protein can be at least one of skim milk powder, concentrated whey protein, casein, hydrolyzed soy protein, glycoprotein, and zein. The fatty acid can be at least one of linseed oil powder, spirulina powder, and lecithin. The colloid can be at least one of gum arabic, pectin, corn syrup, agar, alginate, β-glucan, locust bean gum, and carrageenan. The fiber can be at least one of amylose, microcrystalline cellulose, sorbitol, erythritol, resistant dextrin, oat fiber, citrus fiber, and chicory fiber.

[0053] Please refer to Figure 2 , in an embodiment, the embedding structure 10b of the probiotic of an embodiment of the present invention includes a core 11b and an outer shell 12. Different from Figure 1 the embedding structure 10a of the probiotic shown, Figure 2 the core 11b of the embedding structure 10b of the probiotic shown further includes a third core layer 113, which wraps the second core layer 112, making the core 11b a three-layer structure. In an embodiment, the main component of the third core layer 113 is prebiotics. It can be understood that prebiotics have the effect of promoting the growth of the probiotics in the second core layer 112. For example, the prebiotics can be at least one of fructo-oligosaccharide (FOS), galacto-oligosaccharide (GOS), inulin, stachyose, free xylo-oligosaccharide, soy oligosaccharide, isomaltooligosaccharide, lactulose oligosaccharide, lactulose, raffinose, polydextrin, polydextrose, grape oligosaccharide, mannan oligosaccharide, arabinan oligosaccharide, fuco-oligosaccharide, chito-oligosaccharide, lactitol, and lactulose. In a preferred embodiment, the prebiotics can be at least one of fructo-oligosaccharide, galacto-oligosaccharide, inulin, and stachyose.

[0054] The following describes the manufacturing method of the probiotic embedding structure of this embodiment.

[0055] First, a culture medium is inoculated with probiotics for fermentation. The composition of the culture medium is selected according to the probiotics to be embedded, so it will not be described here. After the fermentation is completed, the culture medium is separated into the probiotic bacteria and the fermentation supernatant by an appropriate method. For example, the probiotic bacteria and the fermentation supernatant can be separated by centrifugation or filtration. Then, the fermentation supernatant is sterilized and dried, and the dried powdered fermentation supernatant can be used as the first core layer 111, as shown in FIG. Figure 1 It is understandable that the separated fermentation supernatant may contain a small amount of probiotic bacteria, but after the sterilization process, the probiotic bacteria lose their activity or become fragments, that is, they cannot split into new probiotics.

[0056] Next, the powdered fermentation supernatant is introduced into a fluidized bed. In one embodiment, the inlet air temperature of the fluidized bed is less than or equal to 60°C, preferably less than or equal to 55°C. In one embodiment, the outlet temperature of the fluidized bed is greater than or equal to 30°C. In one embodiment, the inlet air can be filtered through a membrane to absorb moisture and microorganisms.

[0057] Continuing with the above description, the probiotic bacteria obtained by centrifugation or filtration are in the form of blocks or cakes. The blocks or cakes are diluted with an appropriate liquid to form a fluid state and then atomized and sprayed into the fluidized bed. The atomized bacteria sprayed into the fluidized bed adsorb and coat the first core layer 111. It is understood that the bacteria coated on the outside of the first core layer 111 form the second core layer 112 after drying in the fluidized bed. Figure 1 As shown, multiple cores 11a are formed into particles. In one embodiment, the moisture content of the particles is less than or equal to 7%. Finally, a multi-layered shell 12 is formed to cover the cores 11a. Figure 1 According to the above manufacturing method, the probiotics do not need to be dried in advance, but are dried during the embedding process, which can reduce the heat damage to the probiotics during the processing and maintain better probiotic activity.

[0058] Figure 2 The manufacturing method of the probiotic-encapsulated structure 10b shown is to atomize and spray the prebiotics into a fluidized bed before forming the shell 12, so that the prebiotics coat the second core layer 112. After the prebiotics are dried, a third core layer 113 is formed, and a three-layer core 11b is formed. In one embodiment, the moisture content of the particles at this time is less than or equal to 7%. Finally, the shell 12 is formed to coat the core 11b, forming the following. Figure 2 The encapsulated structure 10b of probiotics is shown.

[0059] The following describes the steps for forming the outer shell 1. Continuing from the previous steps, the first shell material is atomized and sprayed into a fluidized bed so that the first shell material coats the core 11a or 11b. After the first shell material dries, a first shell 121 is formed, as Figure 1 or Figure 2 shown. As described above, the first shell material can be protein, fatty acid, or colloid. In one embodiment, the moisture content of the particles at this time is less than or equal to 7%. It should be noted that the bacteria, prebiotics, protein, fatty acid, or colloid sprayed into the fluidized bed can be adjusted to an appropriate concentration according to fluidity to facilitate atomization. For example, the concentration of bacteria, prebiotics, protein, fatty acid, or colloid can be 15-40% (w / w).

[0060] Next, the particles containing the first shell 121 are collected from the fluidized bed, and the collected particles are uniformly mixed with a second shell material and then dried so that the second shell material coats the first shell 121 to form a second shell 122. In one embodiment, the second shell material can be uniformly mixed with the particles containing the first shell 121 by a shot blasting machine, and then dried by a drying device (such as a drying oven). As described above, the second shell material can be colloid, protein, fatty acid, or fiber. Finally, the particles containing the second shell 122 are uniformly mixed with a third shell material and granulated and then dried, so that the third shell material coats the second shell 122 and forms a third shell 123. In one embodiment, the third shell material is uniformly mixed with the particles containing the second shell 122 by a shot blasting machine and then shot blasted and granulated, and then dried by a drying device (such as a drying oven). As described above, the third shell material can be fiber or colloid.

[0061] Survival test of probiotics in the probiotic embedding structure

[0062] According to the foregoing manufacturing method, an embedded structure of probiotics is prepared with the compositions of Examples 1-9 shown in Table 1, wherein the probiotics include Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, and Lactobacillus acidophilus; postbiotics are the fermentation supernatants of the above probiotic combinations; prebiotics include fructooligosaccharide, galactooligosaccharide, inulin, and stachyose; fatty acids include linseed oil powder, spirulina powder, and lecithin; proteins include skim milk powder, hydrolyzed soy protein, and concentrated whey protein; colloids include gum arabic and pectin; and fibers include amylose, microcrystalline cellulose, sorbitol, erythritol, resistant dextrin, oat fiber, citrus fiber, and Jerusalem artichoke fiber. For the recovered granular probiotic embedded structure, the weight percentage content of postbiotics is about 1-5%, the weight percentage content of probiotics is about 1-5%, the weight percentage content of prebiotics is about 10-20%, the weight percentage content of fatty acids is about 1-5%, the weight percentage content of proteins is about 7-15%, the weight percentage content of colloids is about 2-5%, and the rest is fiber. In the examples without the third core layer (prebiotics), the weight percentage content of postbiotics is about 1-5%, the weight percentage content of probiotics is about 1-5%, the weight percentage content of fatty acids is about 1-5%, the weight percentage content of proteins is about 10-25%, the weight percentage content of colloids is about 3-10%, and the rest is fiber.

[0063] Table 1. Compositions of the probiotic embedded structure

[0064]

[0065] Next, 10 g of the particles of Examples 1-9 are taken and added to 90 g of water, and the particles of Examples 1-9 are dissolved by extrusion with a stirring rod to make a 10-fold dilution. Then, serial dilution is carried out, and 1 ml of the dilution is taken and inoculated into an MRS + 0.05% cysteine medium, and anaerobically cultured at 37 °C for 72 hours, and then the number of bacteria is counted. The number of bacteria and the logarithm of the number of bacteria in the probiotic survival test are shown in Table 2 and Figure 3 as shown. In addition, each example is statistically analyzed with other examples one by one. If there is no significant difference statistically, they are marked with the same English letter, as Figure 3 shown.

[0066] From Figure 3It can be seen that, when the composition of the outer shell 12 is the same, such as in Examples 1-3, Examples 4-6, and Examples 7-9, different core compositions have different protective effects on the activity of probiotics. For example, the core composed of postbiotics, probiotics, and prebiotics from the inside out (Examples 3, 6, 9) has the best protective effect on the activity of probiotics, that is, the number of viable probiotic bacteria is the best, and there are statistically significant differences from the other two groups of examples. In addition, compared with the core composed of probiotics and postbiotics from the inside out (Examples 2, 5, 8), the core composed of postbiotics and probiotics from the inside out (Examples 1, 4, 7) has a better protective effect on the activity of probiotics, and there are statistically significant differences. In other words, setting the probiotics at the innermost core position of the embedding structure does not achieve a better protective effect on the activity of probiotics.

[0067] In addition, when the composition of the core is the same, such as in Examples 1, 4, 7 and Examples 3, 6, 9, different outer shell compositions have different protective effects on the activity of probiotics. For example, compared with the outer shell composition of Example 9, the outer shell compositions of Examples 3 and 6 have a better protective effect on the activity of probiotics, and there are statistically significant differences. Similarly, compared with the outer shell composition of Example 7, the outer shell compositions of Examples 1 and 4 have a better protective effect on the activity of probiotics, and there are statistically significant differences.

[0068] Table 2. Results of the probiotic survival test

[0069]

[0070] From Table 2 and Figure 3 the results show that even with the same core or outer shell materials, changes in the core structure or outer shell structure can significantly improve the protective effect on probiotic activity.

[0071] In summary, the embedding structure of the probiotics of the present invention uses postbiotics as the innermost layer, and then sequentially coats the probiotics and the outer shell, which can effectively improve the protective effect on probiotics. Preferably, coating prebiotics outside the probiotic layer and then coating the outer shell can further improve the protective effect on probiotics. The granular probiotic embedding structure is beneficial for subsequent processing and can avoid choking when users eat.

[0072] The above-described embodiments are only for illustrating the technical ideas and characteristics of the present invention, and the purpose is to enable those skilled in the art to understand the content of the present invention and implement it accordingly. It should not be used to limit the patent scope of the present invention. That is, any equivalent changes or modifications made according to the spirit disclosed in the present invention should still be covered within the patent scope of the present invention.

Claims

1. An embedding structure of probiotics, characterized in that, Comprising: a core and a shell; The core comprises: A first core layer, which is a postbiotic, and the postbiotic is a ferment of the probiotic; and, A second core layer, which coats the first core layer and is an active probiotic; A third core layer, which coats the second core layer and is a prebiotic; The shell comprises a first shell layer, a second shell layer and a third shell layer, wherein the first shell layer coats the core, the second shell layer coats the first shell layer, the third shell layer coats the second shell layer, and the first shell layer is protein and fatty acid, the second shell layer is a colloid, and the third shell layer is a fiber; The fatty acid comprises at least one of linseed oil powder, spirulina powder and lecithin.

2. The embedded structure of the probiotic according to claim 1, characterized in that, The probiotic bacteria include Bifidobacterium animalis subsp. ( Bifidobacterium animalis subsp. lactis ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus acidophilus ( Lactobacillus acidophilus ), Bacillus coagulans ( Bacillus coagulans ), Bifidobacterium bifidum ( Bifidobacterium bifidum ), Bifidobacterium breve ( Bifidobacterium breve ), Bifidobacterium longum ( Bifidobacterium longum ), Lactobacillus brevis ( Lactobacillus brevis ), Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus bulgaricus ), Lactobacillus casei ( Lactobacillus casei ), Lactobacillus fermentum ( Lactobacillus fermentum ), Lactobacillus gasseri ( Lactobacillus gasseri ), Lactobacillus helveticus ( Lactobacillus helveticus ), Lactobacillus johnsonii ( Lactobacillus johnsonii ), Lactobacillus paracasei ( Lactobacillus paracasei ), Lactobacillus pentosus( Lactobacillus pentosus ), Lactobacillus reuteri ( Lactobacillus reuteri ), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus salivarius ( Lactobacillus salivarius ), Lactococcus lactis ( Lactococcus lactis ), Streptococcus thermophilus ( Streptococcus thermophilus ), Pediococcus pentosaceus( Pediococcus pentosaceus ) and Pediococcus acidilactici ( Pediococcus acidilactici ) at least one of them.

3. The embedding structure of the probiotic according to claim 1, characterized in that The probiotic comprises at least one of Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum and Lactobacillus acidophilus.

4. The embedding structure of the probiotic according to claim 1, characterized in that, The prebiotic comprises at least one of fructooligosaccharide, galactooligosaccharide, inulin, stachyose, free xylooligosaccharide, soybean oligosaccharide, isomaltooligosaccharide, lactulose, raffinose, polydextrin, polydextrose, glucooligosaccharide, mannan oligosaccharide, arabinan oligosaccharide, fucooligosaccharide, chitosan oligosaccharide, lactitol.

5. The embedding structure of the probiotic according to claim 1, wherein, In the embedding structure of the probiotics, the weight percentage content of the postbiotics is 1-5%; the weight percentage content of the probiotics is 1-5%; the weight percentage content of the prebiotics is 10-20%; the weight percentage content of the fatty acids is 1-5%, the weight percentage content of the protein is 7-15%, and the weight percentage content of the colloid is 2-5%, and the rest is fiber.

6. The embedding structure of the probiotic according to claim 1, characterized in that, The protein includes at least one of skim milk powder, concentrated whey protein, casein, hydrolyzed soy protein, glycoprotein, and corn protein.

7. The embedding structure of the probiotic according to claim 1, characterized in that, The colloid includes at least one of gum arabic, pectin, corn sugar gum, agar, alginate, β-glucan, locust bean gum, and carrageenan.

8. The embedding structure of the probiotic according to claim 1, wherein, The fiber includes at least one of amylose, microcrystalline cellulose, sorbitol, erythritol, resistant dextrin, oat fiber, citrus fiber, and chicory fiber.

9. A method for preparing an embedding structure of probiotics, characterized in that, It includes: (1) Inoculating probiotics into a culture medium for fermentation; (2) After fermentation is completed, separating the probiotic cells and the fermentation supernatant; (3) Sterilizing and drying the fermentation supernatant, and introducing the powdered fermentation supernatant as the first core layer into a fluidized bed; (4) Diluting the cells into a flowing state and atomizing and spraying them into the fluidized bed so that the cells adsorb and coat the first core layer to form a second core layer; (5) Atomizing and spraying the prebiotics into the fluidized bed so that the prebiotics coat the second core layer to form a third core layer and multiple granular cores; (6) Forming a multi-layered shell for coating the multiple cores; Among them, the step of forming the shell includes: (A) Atomizing and spraying the first shell layer material into the fluidized bed so that the first shell layer material coats the core to form a first shell layer; the first shell layer material is protein and fatty acid; the fatty acid includes at least one of linseed oil powder, spirulina powder, and lecithin; (B) Collecting the particles containing the first shell layer, uniformly mixing them with the second shell layer material and then drying them so that the second shell layer material coats the first shell layer and forms a second shell layer; the second shell layer material is colloid; And (C) Uniformly mixing the particles containing the second shell layer with the third shell layer material, granulating and then drying them so that the third shell layer material coats the second shell layer and forms a third shell layer; the third shell layer material is fiber.

10. The preparation method of the embedding structure of the probiotic according to claim 9, characterized in that, In the embedding structure of the probiotics, the weight percentage content of the fermentation supernatant is 1-5%; the weight percentage content of the probiotics is 1-5%; the weight percentage content of the prebiotics is 10-20%; the weight percentage content of the fatty acids is 1-5%, the weight percentage content of the protein is 7-15%, and the weight percentage content of the colloid is 2-5%, and the rest is fiber.

11. The preparation method of the embedding structure of the probiotic according to claim 9, characterized in that, The second shell layer material is uniformly mixed with the particles containing the first shell layer by a shot blasting machine.

12. The preparation method of the embedding structure of the probiotic according to claim 9, wherein, The third shell layer material is uniformly mixed with the particles containing the second shell layer by a shot blasting machine and then shot blast granulated.

13. The preparation method of the embedding structure of the probiotic as described in claim 9, characterized in that, The inlet air temperature of the fluidized bed is less than or equal to 60 degrees Celsius.

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