Fermenter for culturing CHO cells, which is convenient for cleaning and disinfection

Abstract

The application provides a fermentation tank for CHO cell culture which is convenient to clean and disinfect, belongs to the technical field of fermentation tanks and is used for solving the problem of long cleaning and disinfecting time of existing fermentation tanks. The fermentation tank comprises a bottom plate and a wiping assembly. The upper side of the bottom plate is provided with the fermentation tank. The upper side of the bottom plate is provided with a switching assembly and a collecting assembly. The switching assembly is fixed with a sealing plate. The sealing plate is provided with a spray cleaning assembly. The wiping assembly comprises a U-shaped frame. The U-shaped frame is fixed on the upper side of the sealing plate. The upper side of the U-shaped frame is fixed with a rotary motor. The output shaft of the rotary motor is connected with a mounting pipe through a shaft coupling. The end of the mounting pipe is fixed with two sealing sleeve rods. The side of the sealing sleeve rod is provided with a mounting ring block. The upper side of the mounting ring block is provided with an elastic leather sleeve. The elastic leather sleeve is covered on the sealing sleeve rod. The end of the elastic leather sleeve is fixed with a wiping cotton block. A first side rotary joint is rotatably arranged on the mounting pipe. The fermentation tank for CHO cell culture can be quickly cleaned and disinfected.

Description

Technical Field

[0001] This invention belongs to the field of fermenter technology, and relates to a fermenter for CHO cell culture, particularly a fermenter for CHO cell culture that is easy to clean and disinfect. Background Technology

[0002] Bio-fermentation engineering is an important component of bioengineering, where microorganisms utilize carbohydrates to ferment and produce various industrial solvents and chemical raw materials. Ethanol, acetone-butanol, butanol-isopropanol, acetone-ethanol, 2,3-butanediol, and glycerol fermentation are some forms of solvent fermentation by microorganisms. Bioengineering has a wide range of applications, including agriculture, industry, medicine, pharmacology, energy, environmental protection, metallurgy, and chemical raw materials. Fermentation engineering originated from home-based or workshop-style fermentation (agricultural handicrafts), later borrowing from chemical engineering to achieve industrialized production (modern fermentation engineering), and finally returning to its roots by focusing on the life activities of microorganisms to study, design, and guide industrial fermentation production (modern fermentation engineering), thus entering the realm of bioengineering.

[0003] Currently, biological fermentation is generally carried out in fermenters. During the operation of the fermenter, the material inside the fermenter will adhere to the inner wall of the fermenter. In the existing technology, the fermentation equipment needs to be cleaned and disinfected after fermentation in order to carry out the next fermentation. Generally, a cleaning ball is used to spray the inside of the fermenter with high pressure. However, the spraying position of the cleaning ball is limited, and it is impossible to spray the inner wall of the fermenter evenly. This results in the need for continuous spraying of the inner wall of the fermenter for a long time to clean it. Moreover, the spraying cannot remove all the residues. The existing method is to inject cleaning solution for cleaning and disinfection, which still requires a lot of time. Therefore, we propose a fermenter for CHO cell culture that can be cleaned and disinfected quickly. Summary of the Invention

[0004] The purpose of this invention is to address the aforementioned problems in existing technologies by proposing a fermenter for CHO cell culture that is easy to clean and disinfect. The technical problem to be solved by this device is: how to quickly clean and disinfect the fermenter for CHO cell culture.

[0005] The objective of this invention can be achieved through the following technical solutions: A CHO cell culture fermenter that is easy to clean and disinfect includes a base plate and a wiping assembly. Three fixing frames are fixed above the base plate, and the fermenter is fixed between the three fixing frames. A switching assembly and a collection assembly are arranged above the base plate. A sealing plate is fixed on the switching assembly, and a spray washing assembly is arranged on the sealing plate. The collection assembly is located directly below the fermenter. The wiping assembly includes a U-shaped frame, which is fixed above the sealing plate. A rotary motor is fixed above the U-shaped frame. The output shaft of the rotary motor is connected to an installation pipe through a coupling. Two symmetrical sealing sleeves are fixed to the end of the installation pipe. The sealing sleeves are connected to the installation pipe. A water injection hole is opened at the end of the sealing sleeve. Two fixing rings are fixed to the side of the sealing sleeve. An installation ring block is arranged between the two fixing rings. A telescopic leather sleeve is arranged on the installation ring block. The telescopic leather sleeve covers the sealing sleeve. A wiping cotton block is fixed to the end of the telescopic leather sleeve. The wiping cotton block is fixed to the end of the sealing sleeve. A first side rotary joint is rotatably arranged on the installation pipe and is connected to a water inlet pipe.

[0006] The working principle of this invention is as follows: The switching component drives the sealing plate to descend, the sealing plate drives the spray washing component to move into the fermentation tank, the spray washing component washes the inside of the fermentation tank, after rinsing, the switching component drives the sealing plate to rise, the sealing plate drives the spray washing component to rotate, thereby moving the U-shaped frame to the top of the fermentation tank, the sealing plate drives the U-shaped frame to descend, the U-shaped frame drives the rotary motor to descend, the rotary motor drives the installation pipe to descend, the installation pipe drives the sealing sleeve rod to move into the fermentation tank, the sealing plate seals the fermentation tank, the water inlet pipe sends the cleaning liquid into the inside of the installation pipe, the cleaning liquid moves into the inside of the sealing sleeve rod through the installation pipe, the cleaning liquid drives the sealing sleeve rod to extend through water pressure, the extension of the sealing sleeve rod causes the wiping cotton block to come into contact with the inner wall of the fermentation tank, the cleaning liquid is injected into the inside of the wiping cotton block through the water injection hole, the rotary motor drives the installation pipe to rotate through the output shaft, the installation pipe drives the sealing sleeve rod to rotate, the sealing sleeve rod drives the wiping cotton block to rotate, the wiping cotton block wipes the inner wall of the fermentation tank, after wiping, the fermentation tank discharges wastewater into the collection component, completing the cleaning.

[0007] A fixing plate is fixed between the three fixing frames. The fixing plate is located directly below the fermentation tank. An opening is provided on the top of the fermentation tank. A pressure pipe is provided on the side of the fermentation tank. A discharge pipe is provided at the bottom of the fermentation tank. The discharge pipe passes through the fixing plate. Both the discharge pipe and the pressure pipe are equipped with electrically controlled valves.

[0008] With the above structure, the pressurization pipe is opened to inject high-pressure steam into the fermenter. The high-pressure steam sterilizes the inside of the fermenter, and the fixing plate can enhance the stability of the fixing frame.

[0009] The collection assembly includes a processing box with several casters fixed to the bottom. The casters are located above the base plate. Inside the processing box, from top to bottom, there are a metal mesh filter layer, a sand filter layer, and an activated carbon filter layer. A drain pipe is provided on the side of the processing box, and a shut-off valve is provided on the drain pipe. A cover plate is detachably provided on the top of the processing box, and a water inlet is provided on the cover plate. The end of the discharge pipe is located inside the water inlet.

[0010] With the above structure, sewage is discharged into the treatment tank through the discharge pipe. The metal mesh filter layer, sand filter layer and activated carbon filter layer inside the treatment tank filter the sewage in sequence. Then, the shut-off valve is opened and the drain pipe discharges the filtered sewage.

[0011] The switching assembly includes two electric push rods, both of which are fixed to the base plate. Support plates are fixed to the ends of the two electric push rods. A rotating disk is rotatably mounted above the support plate. A switching motor is fixed below the support plate. The output shaft of the switching motor extends through the support plate and is fixedly connected to the rotating disk. A sealing plate is fixed on the rotating disk.

[0012] With the above structure, the two electric push rods extend and drive the support plate to rise, the support plate drives the rotating disk to rise, the rotating disk drives the sealing plate to rise, and the switching motor drives the rotating disk to rotate through the output shaft, which in turn drives the sealing plate to rotate.

[0013] The spray washing assembly includes a mounting bracket and a power motor. The mounting bracket is U-shaped and fixed above the sealing plate. A connecting pipe is rotatably mounted on the mounting bracket, and a connecting joint is provided at the end of the connecting pipe. The connecting joint is connected to a high-pressure water pipe. A rotating pipe is rotatably mounted on the connecting pipe. A second driven bevel gear is fixed on the connecting pipe, and a first driven bevel gear is fixed on the rotating pipe. The first and second driven bevel gears are symmetrical to each other. The power motor is fixed above the sealing plate, and a driving bevel gear is fixed on the output shaft of the power motor. The driving bevel gear meshes with the first and second driven bevel gears respectively. A circular cylinder is fixed at the end of the rotating pipe.

[0014] With the above structure, the power motor drives the active bevel gear to rotate through the output shaft. The active bevel gear drives the first driven bevel gear and the second driven bevel gear to rotate. The first driven bevel gear drives the rotating tube to rotate, and the second driven bevel gear drives the connecting tube to rotate.

[0015] The spraying assembly also includes a rotating cylinder and two triangular arc blocks. The rotating cylinder is fixed on the connecting pipe and is located inside the annular cylinder. The two triangular arc blocks are symmetrically fixed on the connecting pipe, forming a V-shaped groove between the triangular arc blocks and the rotating cylinder. Several rotating pipes are evenly distributed around the circumference of the annular cylinder. One end of the rotating pipe is fixed with an arc-shaped nozzle. Spray nozzles are provided on the front, upper, and lower sides of the arc-shaped nozzle. The other end of the rotating pipe is fixed with an arc-shaped plate. Two extrusion rods are fixed on the arc-shaped plate and abut against the lower part of the rotating cylinder. A third-side rotating joint is fixed on the connecting pipe. Several connecting pipes are fixed on the third-side rotating joint. Spring hoses are connected to the connecting pipes. A second-side rotating joint is fixed to the end of the spring hose. The second-side rotating joint is rotatably mounted on the rotating pipe at the corresponding position.

[0016] Using the above structure, the connecting pipe drives the rotating cylinder and two triangular arc blocks to rotate. The rotating pipe drives the annular cylinder to rotate in the opposite direction. The annular cylinder drives the rotating pipe to move. The rotating pipe drives the arc plate to move. The arc plate drives the two extrusion rods to move. When one of the extrusion rods moves into the V-groove, the extrusion rod drives the arc plate to flip. The arc plate drives the rotating pipe to rotate. The rotating pipe drives the arc-shaped nozzle to flip. The connecting pipe delivers the cleaning liquid to the third-side rotating joint. The third-side rotating joint delivers the cleaning liquid to the spring hose through the connecting pipe. The spring hose delivers the cleaning liquid to the rotating pipe. The rotating pipe delivers the cleaning liquid to the arc-shaped nozzle. The arc-shaped nozzle sprays the cleaning liquid onto the fermenter through the spray nozzle to rinse the inner wall of the fermenter.

[0017] A control box is fixed above the base plate. The control box is electrically connected to the rotary motor, electric push rod, power motor, switching motor and electric control valve.

[0018] With the above structure, the control box can control the start and stop of the rotary motor, electric push rod, power motor, switching motor and electric valve.

[0019] Compared with existing technologies, this easy-to-clean and disinfect fermenter for CHO cell culture has the following advantages: By combining the fermentation tank and the spraying assembly, the power motor drives the active bevel gear to rotate via the output shaft. The active bevel gear drives the first driven bevel gear and the second driven bevel gear to rotate. The first driven bevel gear drives the rotating tube to rotate, and the second driven bevel gear drives the connecting tube to rotate. The connecting tube drives the rotating cylinder and the two triangular arc blocks to rotate. The rotating tube drives the annular cylinder to rotate in the opposite direction. The annular cylinder drives the rotating tube to move. The rotating tube drives the arc plate to move. The arc plate drives the two extrusion rods to move. When one of the extrusion rods moves into the V-groove, the extrusion rod drives the arc plate to flip. The arc plate drives the rotating tube to rotate. The rotating tube drives the arc-shaped nozzle to flip, which can uniformly spray the inner wall of the fermentation tank, reducing the time required for spraying and cleaning. The fermenter and wiping assembly work together. A water pipe delivers cleaning solution into the installation tube, which then moves the solution to the sealing sleeve. Water pressure causes the sealing sleeve to extend, pressing the wiping cotton against the inner wall of the fermenter. Cleaning solution is then injected into the wiping cotton through the water inlet. A rotary motor drives the installation tube via its output shaft, which in turn rotates the sealing sleeve, which in turn rotates the wiping cotton. The wiping cotton then wipes the inner wall of the fermenter, removing residue without requiring soaking and reducing the time needed for cleaning. Attached Figure Description

[0020] Figure 1 This is a three-dimensional structural schematic diagram of the present invention; Figure 2 This is a front view structural diagram of the present invention; Figure 3 This is a cross-sectional structural schematic diagram of the present invention; Figure 4 This is a schematic diagram of the internal cross-sectional structure of the wiping component in this invention; Figure 5 This is a three-dimensional structural diagram of the spray washing component in this invention; Figure 6 This is a three-dimensional structural diagram of some components of the spray washing assembly in this invention; In the diagram: 1. Rotary motor; 2. Wiping cotton pad; 3. Electric push rod; 4. Fermentation tank; 5. Base plate; 6. Control box; 7. Fixing plate; 8. Processing box; 9. Cover plate; 10. Discharge pipe; 11. Pressurization pipe; 12. Fixing frame; 13. Opening; 14. Arc-shaped nozzle; 15. Circular cylinder; 16. Rotating pipe; 17. Power motor; 18. Drive bevel gear; 19. Connecting joint; 20. U-shaped frame; 21. First side rotary joint; 22. Support plate; 23. Switching motor; 24. Universal wheel; 25. Connecting pipe; 2 6. First driven bevel gear; 27. Second driven bevel gear; 28. Activated carbon filter layer; 29. ​​Rotating disc; 30. Sand and gravel filter layer; 31. Drain pipe; 32. Metal mesh filter layer; 33. Connecting pipe; 34. Spring hose; 35. Rotating cylinder; 36. V-groove; 37. Mounting pipe; 38. Sealing sleeve rod; 39. Fixing ring; 40. Telescopic sleeve; 41. Mounting ring block; 42. Rotating pipe; 43. Second side rotary joint; 44. Triangular arc block; 45. Extruded round rod; 46. Arc plate; 47. Sealing plate. Detailed Implementation

[0021] The technical solution of this patent will be further described in detail below with reference to specific embodiments.

[0022] The embodiments of this patent are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this patent, and should not be construed as limiting this patent.

[0023] In the description of this patent, it should be understood that the terms “center,” “upper,” “lower,” “front,” “back,” “left,” “right,” “vertical,” “horizontal,” “top,” “bottom,” “inner,” and “outer,” etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this patent and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this patent.

[0024] In the description of this patent, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "setting" should be interpreted broadly. For example, they can refer to a fixed connection or setting, a detachable connection or setting, or an integral connection or setting. Those skilled in the art can understand the specific meaning of the above terms in this patent according to the specific circumstances.

[0025] Please see Figure 1-6 This embodiment provides a CHO cell culture fermenter that is easy to clean and disinfect, including a base plate 5 and a wiping assembly. Three fixing brackets 12 are fixed above the base plate 5, and a fermenter 4 is fixed between the three fixing brackets 12. A switching assembly and a collection assembly are arranged above the base plate 5. A sealing plate 47 is fixed on the switching assembly, and a spray washing assembly is arranged on the sealing plate 47. The collection assembly is located directly below the fermenter 4. The wiping assembly includes a U-shaped frame 20, which is fixed above the sealing plate 47. A rotary motor 1 is fixed above the U-shaped frame 20, and the output shaft of the rotary motor 1 is connected to an installation pipe 3 through a coupling. 7. Two symmetrical sealing sleeve rods 38 are fixed to the end of the installation pipe 37. The sealing sleeve rods 38 are connected to the installation pipe 37. A water injection hole is opened at the end of the sealing sleeve rod 38. Two fixing rings 39 are fixed to the side of the sealing sleeve rod 38. An installation ring block 41 is provided between the two fixing rings 39. A telescopic leather sleeve 40 is provided on the installation ring block 41. The telescopic leather sleeve 40 covers the sealing sleeve rod 38. A wiping cotton block 2 is fixed to the end of the telescopic leather sleeve 40. The wiping cotton block 2 is fixed to the end of the sealing sleeve rod 38. A first side rotary joint 21 is rotatably provided on the installation pipe 37. The first side rotary joint 21 is connected to the water inlet pipe.

[0026] The switching component lowers the sealing plate 47, which in turn moves the spraying component into the fermentation tank 4. The spraying component rinses the inside of the fermentation tank 4. After rinsing, the switching component raises the sealing plate 47, which in turn rotates the spraying component, moving the U-shaped frame 20 above the fermentation tank 4. The sealing plate 47 then lowers the U-shaped frame 20, which in turn lowers the rotary motor 1. The rotary motor 1 lowers the mounting pipe 37, which in turn moves the sealing sleeve 38 into the fermentation tank 4. The sealing plate 47 then seals the fermentation tank 4. The water inlet pipe then... The cleaning solution is fed into the installation tube 37 and moves through the installation tube 37 to the inside of the sealing sleeve 38. The cleaning solution, driven by water pressure, causes the sealing sleeve 38 to extend. The extension of the sealing sleeve 38 causes the wiping cotton block 2 to come into contact with the inner wall of the fermenter 4. The cleaning solution is injected into the wiping cotton block 2 through the water injection hole. The rotary motor 1 drives the installation tube 37 to rotate through the output shaft. The installation tube 37 drives the sealing sleeve 38 to rotate, and the sealing sleeve 38 drives the wiping cotton block 2 to rotate. The wiping cotton block 2 wipes the inner wall of the fermenter 4. After wiping, the fermenter 4 discharges wastewater into the collection component, completing the cleaning process.

[0027] A fixing plate 7 is fixed between the three fixing frames 12. The fixing plate 7 is located directly below the fermentation tank 4. An opening 13 is opened on the top of the fermentation tank 4. A pressure pipe 11 is provided on the side of the fermentation tank 4. A discharge pipe 10 is provided at the bottom of the fermentation tank 4. The discharge pipe 10 passes through the fixing plate 7. Both the discharge pipe 10 and the pressure pipe 11 are equipped with electric control valves. When the pressure pipe 11 is opened, high-pressure steam is injected into the interior of the fermentation tank 4. The high-pressure steam sterilizes the interior of the fermentation tank 4. The fixing plate 7 can enhance the stability of the fixing frame 12.

[0028] The collection assembly includes a treatment tank 8, with several casters 24 fixed at the bottom of the treatment tank 8. The casters 24 are located above the base plate 5. Inside the treatment tank 8, from top to bottom, there are a metal mesh filter layer 32, a sand filter layer 30, and an activated carbon filter layer 28. A drain pipe 31 is provided on the side of the treatment tank 8, and a shut-off valve is provided on the drain pipe 31. A cover plate 9 is detachably provided on the top of the treatment tank 8, and a water inlet is opened on the cover plate 9. The end of the discharge pipe 10 is located inside the water inlet. Wastewater is discharged into the interior of the treatment tank 8 through the discharge pipe 10. The metal mesh filter layer 32, the sand filter layer 30, and the activated carbon filter layer 28 inside the treatment tank 8 filter the wastewater in sequence. Then, the shut-off valve is opened, and the drain pipe 31 discharges the filtered wastewater.

[0029] The switching assembly includes two electric push rods 3, both of which are fixed to the base plate 5. Support plates 22 are fixed to the ends of the two electric push rods 3. A rotating disk 29 is rotatably mounted above the support plate 22, and a switching motor 23 is fixed below the support plate 22. The output shaft of the switching motor 23 extends through the support plate 22 and is fixedly connected to the rotating disk 29. A sealing plate 47 is fixed to the rotating disk 29. When the two electric push rods 3 extend, they cause the support plate 22 to rise, which in turn causes the rotating disk 29 to rise. The rotating disk 29 then causes the sealing plate 47 to rise. The switching motor 23 drives the rotating disk 29 to rotate via its output shaft, which in turn causes the sealing plate 47 to rotate.

[0030] The spray washing assembly includes a mounting bracket and a power motor 17. The mounting bracket is U-shaped and fixed above the sealing plate 47. A connecting pipe 25 is rotatably mounted on the mounting bracket, and a connecting joint 19 is provided at the end of the connecting pipe 25, which connects to a high-pressure water pipe. A rotating pipe 16 is rotatably mounted on the connecting pipe 25, and a second driven bevel gear 27 is fixed on the connecting pipe 25. A first driven bevel gear 26 is fixed on the rotating pipe 16. The first driven bevel gear 26 and the second driven bevel gear 27 are symmetrical to each other. The power motor 17 is fixed. Above the sealing plate 47, a drive bevel gear 18 is fixed on the output shaft of the power motor 17. The drive bevel gear 18 meshes with the first driven bevel gear 26 and the second driven bevel gear 27 respectively. A circular ring cylinder 15 is fixed to the end of the rotating tube 16. The power motor 17 drives the drive bevel gear 18 to rotate through the output shaft. The drive bevel gear 18 drives the first driven bevel gear 26 and the second driven bevel gear 27 to rotate. The first driven bevel gear 26 drives the rotating tube 16 to rotate, and the second driven bevel gear 27 drives the connecting tube 25 to rotate.

[0031] The spray washing assembly also includes a rotating cylinder 35 and two triangular arc blocks 44. The rotating cylinder 35 is fixed to the connecting pipe 25 and is located inside the annular cylinder 15. The two triangular arc blocks 44 are symmetrically fixed to the connecting pipe 25, forming a V-groove 36 between the triangular arc blocks 44 and the rotating cylinder 35. Several rotating pipes 42 are evenly distributed around the circumference of the annular cylinder 15. One end of the rotating pipe 42 is fixed with an arc-shaped nozzle 14, which has spray nozzles on its front, upper, and lower sides. The other end of the rotating pipe 42 is fixed with an arc-shaped plate 46, on which two extrusion rods 45 are fixed. The extrusion rods 45 abut against the lower part of the rotating cylinder 35. A third-side rotary joint is fixed to the connecting pipe 25, and several connecting pipes 33 are fixed to the third-side rotary joint. A spring hose 34 is connected to the connecting pipe 33, and a second-side rotary joint 43 is fixed to the end of the spring hose 34. The second-side rotary joint 43 rotates... The rotating tube 42 is placed in the corresponding position; the connecting tube 25 drives the rotating cylinder 35 and the two triangular arc blocks 44 to rotate, the rotating tube 16 drives the annular cylinder 15 to rotate in the opposite direction, the annular cylinder 15 drives the rotating tube 42 to move, the rotating tube 42 drives the arc plate 46 to move, the arc plate 46 drives the two extrusion rods 45 to move, when one of the extrusion rods 45 moves into the V-groove 36, the extrusion rod 45 drives the arc plate 46 to flip, the arc plate 46 drives the rotating tube 42 to rotate, the rotating tube 42 drives the arc nozzle 14 to flip, the connecting tube 25 delivers the cleaning liquid to the third side rotating joint, the third side rotating joint delivers the cleaning liquid to the spring hose 34 through the connecting tube 33, the spring hose 34 delivers the cleaning liquid to the rotating tube 42, the rotating tube 42 delivers the cleaning liquid to the arc nozzle 14, the arc nozzle 14 sprays the cleaning liquid onto the fermenter 4 through the spray nozzle, and rinses the inner wall of the fermenter 4.

[0032] A control box 6 is fixed on the top of the base plate 5. The control box 6 is electrically connected to the rotary motor 1, electric push rod 3, power motor 17, switching motor 23 and solenoid valve. The control box 6 can control the start and stop of the rotary motor 1, electric push rod 3, power motor 17, switching motor 23 and solenoid valve.

[0033] Working principle of the invention: Two electric push rods 3 retract, causing the support plate 22 to descend. The support plate 22 causes the rotating disk 29 to descend, which in turn causes the sealing plate 47 to descend. The sealing plate 47 causes the mounting frame to move, which in turn causes the connecting pipe 25 to move. The connecting pipe 25 causes the rotating pipe 16 to move into the fermentation tank 4. The sealing plate 47 seals the opening 13 of the fermentation tank 4. The high-pressure water pipe injects the cleaning solution into the connecting pipe 25 through the connecting joint 19. The power motor 17 drives the drive bevel gear 18 to rotate through the output shaft. The drive bevel gear 18 drives the first driven bevel gear 26 and the second driven bevel gear 27 to rotate. The first driven bevel gear 26 drives the rotating pipe 16 to rotate, and the second driven bevel gear 27 drives the connecting pipe 25 to rotate. The connecting pipe 25 drives the rotating cylinder 35 and the two triangular arc blocks 44 to rotate. The rotating pipe 16 drives the annular cylinder 15 to rotate. Reverse rotation causes the annular cylinder 15 to move the rotating tube 42, which in turn moves the arc plate 46. The arc plate 46 then moves the two extrusion rods 45. When one of the extrusion rods 45 moves into the V-groove 36, it causes the arc plate 46 to flip. The arc plate 46 then rotates the rotating tube 42, which in turn flips the arc nozzle 14. The connecting pipe 25 delivers the cleaning fluid to the third-side rotary joint, which then delivers the cleaning fluid through the connecting pipe 33 to the spring hose 34. The spring hose 34 delivers the cleaning fluid to the rotating tube 42, which then delivers it to the arc nozzle 14. The arc nozzle 14 sprays the cleaning fluid onto the fermenter 4 through the spray nozzle, rinsing the inner wall of the fermenter 4. Wastewater is discharged into the treatment tank 8 through the discharge pipe 10. After rinsing, the two electric push rods 3 extend, causing the support plate 22 to rise. The support plate 22 then causes the rotating disk 29 to rise, which in turn causes the sealing plate 47 to rise. The sealing plate 47 causes the mounting frame to rise, which in turn causes the connecting pipe 25 to rise. The mounting frame and connecting pipe 25 move out of the fermentation tank 4. The switching motor 23 drives the rotating disk 29 to rotate via its output shaft. The rotating disk 29 then drives the sealing plate 47 to rotate, thereby moving the U-shaped frame 20 above the fermentation tank 4. The sealing plate 47 causes the U-shaped frame 20 to descend, which in turn causes the rotary motor 1 to descend. The rotary motor 1 then causes the mounting pipe 37 to descend, which in turn causes the sealing sleeve 38 to move into the fermentation tank 4. The sealing plate 47 then seals the fermentation tank 4. The water inlet pipe delivers the cleaning solution into the mounting pipe 37, and the cleaning solution moves through the mounting pipe 37 into the sealing sleeve 38. In the first part, the cleaning fluid, driven by water pressure, extends the sealing sleeve rod 38, causing the wiping cotton block 2 to contact the inner wall of the fermenter 4. The cleaning fluid is injected into the wiping cotton block 2 through the water injection hole. The rotary motor 1 drives the installation pipe 37 to rotate through the output shaft. The installation pipe 37 drives the sealing sleeve rod 38 to rotate, which in turn drives the wiping cotton block 2 to rotate. The wiping cotton block 2 wipes the inner wall of the fermenter 4. After wiping, the fermenter 4 discharges the wastewater into the treatment tank 8 through the discharge pipe 10. The metal mesh filter layer 32, the sand filter layer 30, and the activated carbon filter layer 28 inside the treatment tank 8 filter the wastewater in sequence. Then, the shut-off valve is opened, and the drain pipe 31 discharges the filtered wastewater. After that, the pressurization pipe 11 is opened to inject high-pressure steam into the fermenter 4. The high-pressure steam sterilizes the inside of the fermenter 4, completing the cleaning and sterilization process.

[0034] The preferred embodiments of this patent have been described in detail above. However, this patent is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this patent.

Claims

1. A fermenter for CHO cell culture that is easy to clean and disinfect, comprising a base plate (5) and a wiping assembly, characterized in that, Three fixing frames (12) are fixed above the base plate (5), and a fermentation tank (4) is fixed between the three fixing frames (12). A switching assembly and a collection assembly are set above the base plate (5). A sealing plate (47) is fixed on the switching assembly, and a spraying assembly is set on the sealing plate (47). The collection assembly is located directly below the fermentation tank (4). The wiping assembly includes a U-shaped frame (20), which is fixed above the sealing plate (47). A rotary motor (1) is fixed above the U-shaped frame (20). The output shaft of the rotary motor (1) is connected to an installation tube (37) through a coupling. Two symmetrical sealing sleeves are fixed at the end of the installation tube (37). (38), the sealing sleeve (38) is connected to the installation pipe (37), the end of the sealing sleeve (38) is provided with a water injection hole, the side of the sealing sleeve (38) is fixed with two fixing rings (39), the installation ring block (41) is provided between the two fixing rings (39), the installation ring block (41) is provided with a telescopic leather sleeve (40), the telescopic leather sleeve (40) covers the sealing sleeve (38), the end of the telescopic leather sleeve (40) is fixed with a wiping cotton block (2), the wiping cotton block (2) is fixed to the end of the sealing sleeve (38), the installation pipe (37) is rotatably provided with a first side rotary joint (21), the first side rotary joint (21) is connected to the water inlet pipe; A fixing plate (7) is fixed between the three fixing frames (12). The fixing plate (7) is located directly below the fermentation tank (4). An opening (13) is opened on the top of the fermentation tank (4). A pressure pipe (11) is provided on the side of the fermentation tank (4). A discharge pipe (10) is provided at the bottom of the fermentation tank (4). The discharge pipe (10) passes through the fixing plate (7). Both the discharge pipe (10) and the pressure pipe (11) are equipped with electric control valves. The switching assembly includes two electric push rods (3), both of which are fixed on the base plate (5). The ends of the two electric push rods (3) are fixed with a support plate (22). A rotating disk (29) is rotatably arranged above the support plate (22). A switching motor (23) is fixed below the support plate (22). The output shaft of the switching motor (23) extends through the support plate (22). The output shaft of the switching motor (23) is fixedly connected to the rotating disk (29). A sealing plate (47) is fixed on the rotating disk (29).

2. The fermenter for CHO cell culture that is easy to clean and disinfect according to claim 1, characterized in that, The collection assembly includes a processing box (8), with several casters (24) fixed below the processing box (8). The casters (24) are located above the base plate (5). The interior of the processing box (8) is provided with a metal mesh filter layer (32), a sand and gravel filter layer (30), and an activated carbon filter layer (28) arranged from top to bottom. A drain pipe (31) is provided on the side of the processing box (8), and a shut-off valve is provided on the drain pipe (31). A cover plate (9) is detachably provided on the top of the processing box (8), and a water inlet is provided on the cover plate (9). The end of the discharge pipe (10) is located inside the water inlet.

3. The fermenter for CHO cell culture that is easy to clean and disinfect according to claim 1, characterized in that, The spray washing assembly includes a mounting bracket and a power motor (17). The mounting bracket is U-shaped and fixed above the sealing plate (47). A connecting pipe (25) is rotatably mounted on the mounting bracket. A connecting joint (19) is provided at the end of the connecting pipe (25). The connecting joint (19) is connected to a high-pressure water pipe. A rotating pipe (16) is rotatably mounted on the connecting pipe (25). A second driven bevel gear (27) is fixed on the connecting pipe (25). A first driven bevel gear (26) is fixed on the rotating pipe (16). The first driven bevel gear (26) and the second driven bevel gear (27) are symmetrical to each other. The power motor (17) is fixed above the sealing plate (47). An active bevel gear (18) is fixed on the output shaft of the power motor (17). The active bevel gear (18) meshes with the first driven bevel gear (26) and the second driven bevel gear (27) respectively. A circular ring cylinder (15) is fixed at the end of the rotating pipe (16).

4. A fermenter for CHO cell culture that is easy to clean and disinfect according to claim 3, characterized in that, The spraying assembly also includes a rotating cylinder (35) and two triangular arc blocks (44). The rotating cylinder (35) is fixed on the connecting pipe (25) and is located inside the annular cylinder (15). The two triangular arc blocks (44) are symmetrically fixed on the connecting pipe (25), and a V-groove (36) is formed between the triangular arc blocks (44) and the rotating cylinder (35). Several rotating pipes (42) are evenly distributed around the circumference of the annular cylinder (15). An arc-shaped nozzle (14) is fixed at one end of the rotating pipe (42). The front, upper, and lower sides of the arc-shaped nozzle (14) are all... A spray nozzle is provided. An arc plate (46) is fixed at the other end of the rotating pipe (42). Two extrusion rods (45) are fixed on the arc plate (46). The extrusion rods (45) abut against the bottom of the rotating cylinder (35). A third-side rotary joint is fixed on the connecting pipe (25). Several connecting pipes (33) are fixed on the third-side rotary joint. A spring hose (34) is connected to the connecting pipe (33). A second-side rotary joint (43) is fixed at the end of the spring hose (34). The second-side rotary joint (43) is rotatably set on the rotating pipe (42) at the corresponding position.

5. A fermenter for CHO cell culture that is easy to clean and disinfect according to claim 4, characterized in that, A control box (6) is fixed above the base plate (5). The control box (6) is electrically connected to the rotary motor (1), electric push rod (3), power motor (17), switching motor (23) and electric control valve.

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