Chinese herbal medicine enzyme composition and application thereof

Abstract

The application discloses a Chinese herbal medicine enzyme composition and application thereof; the composition comprises a mesophilic alpha-1,4-amylase, a pullanase, a ferulic acid esterase, a glucose oxidase, a catalase and a Boerhaave dispersing composite preparation; each component in the composition has a strong synergistic combination effect, can synergistically improve a feed nutrient digestion rate of a Langde goose, improve growth performance, increase an unsaturated fatty acid content in a Langde goose liver and improve goose liver quality, and increase breeding benefits.

Description

Technical Field

[0001] This invention belongs to the field of animal feed additives, and more specifically, relates to a traditional Chinese medicine enzyme composition for increasing the unsaturated fatty acid content of Landes goose liver and its application. Background Technology

[0002] The Landes goose, also known as the Southwest Grey Goose, belongs to the order Anseriformes and family Anatidae, just like common geese. Originating in the Landes region of southwestern France near the Bay of Biscay, it is a world-renowned breed specifically for foie gras production. A hybrid of wild geese and common geese, it retains some physiological characteristics of its ancestor, the common goose, and can store energy in its liver through large intake of food. Currently, the farming industry uses force-feeding high-energy feed to promote the deposition of large amounts of fat in the liver of Landes geese, resulting in a special fatty liver that is 5 to 10 times larger than a normal liver.

[0003] Landes goose fatty liver is tender and flavorful, containing a large amount of unsaturated fatty acids and various vitamins. Studies have confirmed that unsaturated fatty acids are beneficial to health, lowering cholesterol levels in the blood and having effects such as reducing blood lipids, softening blood vessels, preventing aging, and preventing cardiovascular and cerebrovascular diseases. Therefore, the unsaturated fatty acid content in Landes goose liver affects its quality, and research and measures in this area are insufficient; improving goose liver quality is a key concern in the industry. Furthermore, research has shown that overfeeding or consuming high-concentration feed reduces the digestibility of nutrients in poultry, leading to the excretion of undigested nutrients, resulting in poor production performance, feed waste, and environmental pollution.

[0004] To meet the supply demand for foie gras, improve the quality and breeding efficiency of foie gras, and reduce feed waste and environmental pollution, it is of great significance to explore methods to improve the nutrient digestibility of Landes goose feed and increase the unsaturated fatty acid content of Landes goose liver. Summary of the Invention

[0005] The purpose of this invention is to provide a traditional Chinese medicine enzyme composition for improving the content of unsaturated fatty acids and its application. This traditional Chinese medicine enzyme composition can improve the digestibility of goose feed and increase the content of unsaturated fatty acids in goose liver.

[0006] The technical solution adopted in this invention is:

[0007] In a first aspect, the present invention provides a composition comprising the following raw materials in parts by weight: 1-9 parts of medium-temperature α-1,4-amylase, 1-3 parts of pullulanase, 1-3 parts of ferulic acid esterase, 2-4 parts of glucose oxidase, 1-3 parts of catalase, and 2-12 parts of a compound preparation of berberine.

[0008] In some preferred embodiments of the present invention, the composition comprises the following raw materials in parts by weight: 4 parts of medium-temperature α-1,4-amylase, 2 parts of pullulanase, 2 parts of ferulic acid esterase, 3 parts of glucose oxidase, 2 parts of catalase, and 6 parts of Boluo Huisan compound preparation.

[0009] In some embodiments of the present invention, the mass ratio of the Boluohuisan compound preparation and catalase is (2-4):1.

[0010] In some preferred embodiments of the present invention, the mass ratio of the Boluo Huisan compound preparation to catalase is 3:1.

[0011] In some embodiments of the present invention, the mass ratio of the mesophilic α-1,4-amylase to ferulic acid esterase is (1-3):1.

[0012] In some preferred embodiments of the present invention, the mass ratio of the mesothermal α-1,4-amylase to ferulic acid esterase is 2:1.

[0013] In some embodiments of the present invention, the content of sanguinarine in the compound preparation of Boluo Huisan is 0.1-0.2%.

[0014] In some preferred embodiments of the present invention, the content of sanguinarine in the compound preparation of Boluo Huisan is 0.15%.

[0015] In some embodiments of the present invention, the activity of the mesophilic α-1,4-amylase is 800-1200 U / g, the activity of pullulanase is 800-1200 U / g, the activity of ferulic acid esterase is 800-1200 U / g, the activity of glucose oxidase is 4500-5500 U / g, and the activity of catalase is 4500-5500 U / g.

[0016] In some preferred embodiments of the present invention, the activity of the mesophilic α-1,4-amylase is 1000 U / g, the activity of pullulanase is 1000 U / g, the activity of ferulic acid esterase is 1000 U / g, the activity of glucose oxidase is 5000 U / g, and the activity of catalase is 5000 U / g.

[0017] In some embodiments of the present invention, the composition further includes 13 to 42 parts of a carrier.

[0018] In some preferred embodiments of the present invention, the composition further includes 31 parts of a carrier.

[0019] In some embodiments of the present invention, the carrier comprises silica and / or corn cob powder.

[0020] A second aspect of the invention provides the use of the composition described in the first aspect of the invention in at least one of the following:

[0021] (1) Improve the digestibility of feed nutrients in Landes geese;

[0022] (2) Prepare products that improve the digestibility of feed for Landes geese;

[0023] (3) Increase the content of unsaturated fatty acids in Landes goose liver;

[0024] (4) Prepare products that increase the content of unsaturated fatty acids in Landes goose liver;

[0025] (5) Promotes the growth of Landes geese;

[0026] (6) Prepare products that promote the growth of Landes geese.

[0027] A third aspect of the present invention provides a feed additive comprising the composition described in the first aspect of the present invention.

[0028] In a fourth aspect, the present invention provides a feed containing the feed additives described in the third aspect of the present invention.

[0029] In some embodiments of the present invention, the amount of the feed additive added is 1-2 kg / 1000 kg.

[0030] In some preferred embodiments of the present invention, the amount of the feed additive added is 1.5 kg / 1000 kg.

[0031] A fifth aspect of the present invention provides a method for improving the digestibility of feed nutrients in Landes geese and / or increasing the content of unsaturated fatty acids in the liver of Landes geese and / or promoting the growth of Landes geese, the method comprising the step of feeding Landes geese the composition described in the first aspect of the present invention.

[0032] The beneficial effects of this invention are:

[0033] This invention provides a herbal enzyme composition that can improve the digestibility of feed nutrients and the content of unsaturated fatty acids in goose liver of Landes geese. The composition includes mesophilic α-1,4-amylase, pullulanase, ferulic acid esterase, glucose oxidase, catalase, and a compound preparation of *Bolohuisan*. The mesophilic α-1,4-amylase in the composition improves starch digestibility, while ferulic acid esterase degrades xylan branches, indirectly improving feed nutrient digestibility. Through combination, they synergistically improve the digestibility of feed nutrients in Landes geese and enhance growth performance. *Bolohuisan* has anti-inflammatory effects, and catalase has antioxidant effects; through combination, they synergistically increase the content of unsaturated fatty acids in Landes goose liver, improving the quality of Landes goose foie gras. The components in the composition have a strong synergistic effect, greatly enhancing the overall performance of each component. This composition can be used in the preparation of Landes goose feed. When Landes geese consume feed supplemented with this herbal enzyme composition, nutrient digestibility and absorption are effectively improved, the content of unsaturated fatty acids in goose liver is increased, goose liver quality is improved, and breeding efficiency is increased. Detailed Implementation

[0034] The following will describe the concept and technical effects of the present invention clearly and completely with reference to embodiments, so as to fully understand the purpose, features and effects of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are all within the scope of protection of the present invention.

[0035] The compound preparation of Boluo Huisan is sourced from Hunan Mekeda Company, and the active ingredient, sanguinarine, contains 0.15%.

[0036] All enzymes used were obtained from Guangdong Yiduoli Biotechnology Co., Ltd., and their activities were as follows: 1000 U / g for mesophilic α-1,4-amylase, 1000 U / g for pullulanase, 1000 U / g for ferulic acid esterase, 5000 U / g for glucose oxidase, and 5000 U / g for catalase.

[0037] A method for preparing a traditional Chinese medicine enzyme complex that improves feed digestibility and increases the unsaturated fatty acid content in Landes geese liver includes the following steps:

[0038] Weigh equal weights of the Boluo Huisan composite preparation and the carrier silica, and mix them to obtain mixture one;

[0039] Weigh equal weights of carrier silica and enzymes (medium-temperature α-1,4-amylase, pullulanase, ferulic acid esterase, glucose oxidase, catalase), mix them thoroughly to obtain mixtures two, three, four, five, and six.

[0040] Mixtures 2, 3, 4, 5, and 6, along with the carrier silica, are mixed thoroughly, and then finally mixed with mixture 1 to obtain the herbal enzyme preparation.

[0041] The mixing process can be carried out using a vertical mixer, a single-shaft or twin-shaft horizontal mixer, and the mixing time is 5 to 15 minutes depending on the type of mixer selected, to obtain the herbal enzyme preparation.

[0042] Example 1

[0043] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 1 part of medium-temperature α-1,4-amylase, 1 part of pullulanase, 1 part of ferulic acid esterase, 2 parts of glucose oxidase, 1 part of catalase, 2 parts of Boluo Huisan compound preparation, and 42 parts of silica.

[0044] Example 2

[0045] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 9 parts of medium-temperature α-1,4-amylase, 3 parts of pullulanase, 3 parts of ferulic acid esterase, 4 parts of glucose oxidase, 3 parts of catalase, 12 parts of Boluo Huisan compound preparation, and 13 parts of silica.

[0046] Example 3

[0047] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 4 parts of medium-temperature α-1,4-amylase, 2 parts of pullulanase, 2 parts of ferulic acid esterase, 3 parts of glucose oxidase, 2 parts of catalase, 6 parts of Boluo Huisan compound preparation, and 31 parts of silicon dioxide.

[0048] Comparative Example 1

[0049] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 6 parts of mesophilic α-1,4-amylase, 2 parts of pullulanase, 3 parts of glucose oxidase, 2 parts of catalase, 6 parts of a compound preparation of berberine and ferulate, and 31 parts of silica. The difference between Comparative Example 1 and Example 3 is that ferulic acid esterase was not added, while the amount of mesophilic α-1,4-amylase added is the sum of the amounts of mesophilic α-1,4-amylase and ferulic acid esterase added in Example 3.

[0050] Comparative Example 2

[0051] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: pullulanase 2 parts, ferulic acid esterase 6 parts, glucose oxidase 3 parts, catalase 2 parts, a compound preparation of berberine and ferulate extract 6 parts, and silica 31 parts. The difference between Comparative Example 2 and Example 3 is that mesophilic α-1,4-amylase was not added, while the amount of ferulic acid esterase added is the sum of the amounts of mesophilic α-1,4-amylase and ferulic acid esterase added in Example 3.

[0052] Comparative Example 3

[0053] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 4 parts of mesophilic α-1,4-amylase, 2 parts of pullulanase, 5 parts of ferulic acid esterase, 3 parts of glucose oxidase, 2 parts of catalase, 6 parts of a compound preparation of *Boluo Huisan*, and 28 parts of silica. The difference between Comparative Example 3 and Example 3 is that the ratio of mesophilic α-1,4-amylase to ferulic acid esterase is less than 1:1.

[0054] Comparative Example 4

[0055] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 4 parts of mesophilic α-1,4-amylase, 2 parts of pullulanase, 1 part of ferulic acid esterase, 3 parts of glucose oxidase, 2 parts of catalase, 6 parts of a compound preparation of *Boluo Huisan*, and 32 parts of silica. The difference between Comparative Example 4 and Example 3 is that the ratio of mesophilic α-1,4-amylase to ferulic acid esterase is higher than 3:1.

[0056] Comparative Example 5

[0057] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 4 parts of medium-temperature α-1,4-amylase, 2 parts of pullulanase, 2 parts of ferulic acid esterase, 3 parts of glucose oxidase, 8 parts of Boluo Huisan compound preparation, and 31 parts of silica. The difference between Comparative Example 5 and Example 3 is that catalase was not added, while the amount of Boluo Huisan compound preparation added is the sum of the amounts of catalase and Boluo Huisan compound preparation added in Example 3.

[0058] Comparative Example 6

[0059] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 4 parts of medium-temperature α-1,4-amylase, 2 parts of pullulanase, 2 parts of ferulic acid esterase, 3 parts of glucose oxidase, 8 parts of catalase, and 31 parts of silicon dioxide. The difference between Comparative Example 6 and Example 3 is that the Boluo Huisan compound preparation was not added, while the amount of catalase added is the sum of the amounts of catalase and Boluo Huisan compound preparation added in Example 3.

[0060] Comparative Example 7

[0061] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 4 parts of medium-temperature α-1,4-amylase, 2 parts of pullulanase, 2 parts of ferulic acid esterase, 3 parts of glucose oxidase, 6 parts of catalase, 6 parts of a compound preparation of *Boluo Huisan*, and 27 parts of silica. The difference between Comparative Example 7 and Example 3 is that the ratio of the *Boluo Huisan* compound preparation to catalase is less than 2:1.

[0062] Comparative Example 8

[0063] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 4 parts of medium-temperature α-1,4-amylase, 2 parts of pullulanase, 2 parts of ferulic acid esterase, 3 parts of glucose oxidase, 1 part of catalase, 6 parts of a compound preparation of *Boluo Huisan*, and 32 parts of silica. The difference between Comparative Example 8 and Example 3 is that the ratio of the *Boluo Huisan* compound preparation to catalase is higher than 4:1.

[0064] Comparative Example 9

[0065] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 0.5 parts of medium-temperature α-1,4-amylase, 0.5 parts of pullulanase, 0.5 parts of ferulic acid esterase, 1 part of glucose oxidase, 0.5 parts of catalase, 1 part of a compound preparation of *Boluo Huisan*, and 46 parts of silica. The difference between Comparative Example 9 and Example 3 is that the added parts of all components are lower than the minimum added parts of each component in this invention.

[0066] Comparative Example 10

[0067] A traditional Chinese medicine enzyme preparation is made from the following raw materials in parts by weight: 10 parts of medium-temperature α-1,4-amylase, 10 parts of pullulanase, 4 parts of ferulic acid esterase, 5 parts of glucose oxidase, 4 parts of catalase, 13 parts of a compound preparation of *Boluo Huisan*, and 7 parts of silica. The difference between Comparative Example 10 and Example 3 is that the added parts of all components are higher than the maximum added parts of each component in this invention.

[0068] Example 1: The effect of traditional Chinese medicine enzyme preparations on improving feed nutrient digestibility

[0069] Two hundred and sixty healthy, active, and uniform 55-day-old Landes geese were selected and divided into 13 treatment groups, with four replicates per treatment and five geese per replicate. The basal feed formula for the Landes geese consisted of 1000 kg corn, 4 kg goose fat, 3 kg salt, and 1 kg multivitamins. The corn was washed, boiled until 70% cooked, crushed into coarse particles, and mixed evenly with the other ingredients before being force-fed using a pneumatic trolley feeder. Force-feeding began after a 5-day pre-feeding period. The 13 treatment groups were fed a basal feed supplemented with the herbal enzyme preparations from Examples 1-3 and Comparative Examples 1-10; the amount of herbal enzyme preparation added was 1.5 kg / 1000 kg.

[0070] The force-feeding procedure is based on patent document CN202110363486. On Day 1, feed once a day at 150g / meal. On Day 2, feed 160g / meal at 07:00 and 19:00 respectively. On Day 3, feed 180g / meal at 07:00, 13:00, and 19:00 respectively. On Day 4, feed 190g / meal at 07:00, 13:00, and 19:00 respectively. From Day 5 to Day 11, feed once a day at 01:00, 07:00, 13:00, and 19:00, with 200g fed per meal on Day 5, and thereafter increasing the feed amount by 30g per meal each day. From day 12 to day 30, feed once at 04:00, 08:00, 13:00, 18:00 and 23:00. On day 12, feed 410g per meal. After that, increase the amount of feed by 20g per meal each day.

[0071] The apparent digestibility of nutrients was determined by adding 0.5% Cr2O3 as an exogenous indicator to the diet. The main focus was on the apparent digestibility of nutrients during the final stages of the experiment (days 27, 28, 29, and 30 of force-feeding). Excrement was collected on days 27, 28, 29, and 30, in replicates, every 8 hours. Immediately after collection, 10% hydrochloric acid was added for nitrogen fixation. After thorough mixing, 15% of the fresh fecal and urine samples were collected and refrigerated at 4°C. The fecal samples collected from each of the four replicates were then mixed thoroughly, dried to constant weight in a 65°C oven, rehydrated at room temperature for 24 hours, pulverized, and passed through a 40-mesh sieve for later testing. 150g of feed was collected daily during force-feeding. The feed samples collected over the four days were mixed thoroughly, pulverized, and passed through a 40-mesh sieve for later testing. The nutrient digestibility calculation formula is as follows:

[0072] The digestibility of a nutrient (%) is calculated as follows: [1 - (b × c) / (a ​​× d)] × 100. Where a is the content of the nutrient in the diet (%); b is the content of the nutrient in the fecal sample (%); c is the chromium content in the diet (%); and d is the chromium content in the fecal sample (%).

[0073] The results of feed nutrient digestibility are shown in Table 1.

[0074] Table 1. Results of nutrient digestibility determination (%)

[0075]

[0076] Note: Data in the same column with the same letter or no letter indicates no significant difference (P>0.05); data in the same column with different letters indicates significant difference (P<0.05).

[0077] As shown in Table 1, on the one hand, the nutrient digestibility of Examples 1-3 and Comparative Examples 3-4 was higher than that of Comparative Examples 1-2, confirming that mesophilic α-1,4-amylase and ferulic acid esterase synergistically promote nutrient digestion and absorption; the nutrient digestibility of Examples 1-3 and Comparative Examples 7-8 was higher than that of Comparative Examples 5-6, confirming that the Boluo Huisan compound preparation and catalase also synergistically promote nutrient digestion and absorption. On the other hand, compared with Comparative Examples 1, 2, 5, and 6, which lacked any one of the components of mesophilic α-1,4-amylase, ferulic acid esterase, Boluo Huisan compound preparation, and catalase, the nutrient digestibility of Comparative Examples 1-3 and Comparative Examples 3, 4, 7, and 8, which had complete herbal enzyme complex components, was significantly lower than that of Examples 1-3 and Comparative Examples 3, 4, 7, and 8, revealing that there is a synergistic effect among the four enzymes—mesophilic α-1,4-amylase, ferulic acid esterase, Boluo Huisan compound preparation, and catalase—in promoting nutrient digestion and absorption.

[0078] The nutrient digestibility of comparative groups 3-4 was lower than that of examples 1-3, which confirms that the optimal ratio of α-1,4-amylase to ferulic acid esterase in the medium temperature range of (1-3):1 is the best for improving nutrient digestibility.

[0079] The nutrient digestibility of comparative groups 7-8 was lower than that of examples 1-3, confirming that the optimal ratio of the compound preparation of Boluo Huisan to catalase in the range of (2-4):1 was the best for improving nutrient digestibility.

[0080] The nutrient digestibility of Examples 1-3 was higher than that of Comparative Examples 9-10, confirming that there is a certain range for the amount of each component added, and exceeding or falling short of this range will reduce the nutrient digestibility.

[0081] Example 2: Effect of herbal enzyme preparations on the content of unsaturated fatty acids in Landes goose foie gras

[0082] Two hundred and sixty healthy, active, and uniform 55-day-old Landes geese were selected and divided into 13 treatment groups, with four replicates per treatment and five geese per replicate. The basal feed formula for the Landes geese consisted of 1000 kg corn, 4 kg goose fat, 3 kg salt, and 1 kg multivitamins. The corn was washed, boiled until 70% cooked, crushed into coarse particles, and mixed evenly with the other ingredients before being force-fed using a pneumatic trolley feeder. Force-feeding began after a 5-day pre-feeding period. The 13 treatment groups were fed the basal feed supplemented with Examples 1-3 and Comparative Examples 1-10, respectively.

[0083] The feeding and management were the same as in Example 4. After 30 days of force-feeding, all geese were fasted for 12 hours (with free access to water) before slaughter. One female goose was selected from each replicate, and its fatty liver was used for fatty acid analysis. Liver lipids were extracted using the chloroform / methanol method, and fatty acid composition was analyzed by fatty acid methyl esters (FAMEs). FAMEs were prepared using boron trichloride methanol, and their components were analyzed by gas chromatography on an HP-5MS column (30m × 0.25mm × 0.2μm).

[0084] The fatty acid content of Landes goose liver is shown in Table 2.

[0085] Table 2 Liver fatty acid content (g / 100g total fatty acids)

[0086]

[0087] Note: Data in the same column with the same letter or no letter indicates no significant difference (P>0.05); data in the same column with different letters indicates significant difference (P<0.05).

[0088] Linoleic acid is a type of unsaturated fatty acid and is an essential fatty acid for human and animal nutrition.

[0089] As shown in Table 2, on the one hand, the contents of linoleic acid, monounsaturated fatty acids, polyunsaturated fatty acids, and total unsaturated fatty acids in the liver of Examples 1-3 and Comparative Examples 3-4 were all higher than those in Comparative Examples 1-2, confirming that the mesothermal α-1,4-amylase and ferulic acid esterase synergistically increased the content of unsaturated fatty acids in Landes goose liver; on the other hand, the contents of linoleic acid, monounsaturated fatty acids, polyunsaturated fatty acids, and total unsaturated fatty acids in the liver of Examples 1-3 and Comparative Examples 7-8 were all higher than those in Comparative Examples 5-6, confirming that the compound preparation of Boluo Huisan and catalase also synergistically increased the content of unsaturated fatty acids in Landes goose liver. On the other hand, Comparative Examples 1, 2, 5, and 6 lacked any one of the components of mesophilic α-1,4-amylase, ferulic acid esterase, Boluo Huisan compound preparation, and catalase, respectively. The contents of linoleic acid, monounsaturated fatty acids, polyunsaturated fatty acids, and total unsaturated fatty acids in the livers of these groups were significantly lower than those in Examples 1-3 and Comparative Examples 3, 4, 7, and 8, which contained the complete herbal enzyme complex components of the present invention. This reveals that there is a synergistic effect among the four enzymes—mesophilic α-1,4-amylase, ferulic acid esterase, Boluo Huisan compound preparation, and catalase—increasing the content of unsaturated fatty acids in Landes goose liver.

[0090] The contents of linoleic acid, monounsaturated fatty acids, polyunsaturated fatty acids, and total unsaturated fatty acids in the liver of comparative groups 3 and 4 were lower than those in groups 1 and 3 of Examples, confirming that the optimal ratio of α-1,4-amylase to ferulic acid esterase in the medium temperature range of (1-3):1 is the best for increasing the content of unsaturated fatty acids in the liver.

[0091] The contents of linoleic acid, monounsaturated fatty acids, polyunsaturated fatty acids, and total unsaturated fatty acids in the liver of comparative groups 7-8 were lower than those in groups 1-3 of Examples, confirming that the optimal ratio of the compound preparation of Boluo Huisan to catalase in the range of (2-4):1 is the best for increasing the content of unsaturated fatty acids in the liver.

[0092] The results of Examples 1-3 showed that the contents of linoleic acid, monounsaturated fatty acids, polyunsaturated fatty acids, and total unsaturated fatty acids in the liver were higher than those in Comparative Examples 9-10, confirming that there is a certain range for the amount of each component added. Exceeding or falling short of this range will reduce the content of unsaturated fatty acids in the liver.

[0093] The above detailed embodiments have provided a comprehensive description of the present invention. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention. Furthermore, unless otherwise specified, the embodiments of the present invention and the features thereof can be combined with each other.

Claims

1. The use of a composition in at least one of the following: (1) Increase the content of unsaturated fatty acids in Landes goose liver; (2) Prepare products that increase the content of unsaturated fatty acids in Landes goose liver; The composition comprises the following raw materials in parts by weight: 1-9 parts of medium-temperature α-1,4-amylase, 1-3 parts of pullulanase, 1-3 parts of ferulic acid esterase, 2-4 parts of glucose oxidase, 1-3 parts of catalase, 2-12 parts of Boluo Huisan compound preparation, and 13-42 parts of carrier. The mass ratio of the compound preparation of Boluo Huisan to catalase is (2-4):1; The mass ratio of the mesophilic α-1,4-amylase to ferulic acid esterase is (1-3):

1.

2. The application according to claim 1, characterized in that, The content of sanguinarine in the compound preparation of Boluo Huisan is 0.1-0.2%.

3. The application according to claim 1, characterized in that, The activity of the mesophilic α-1,4-amylase is 800–1200 U / g, the activity of pullulanase is 800–1200 U / g, the activity of ferulic acid esterase is 800–1200 U / g, the activity of glucose oxidase is 4500–5500 U / g, and the activity of catalase is 4500–5500 U / g.

4. The application according to claim 1, characterized in that, The carrier includes silicon dioxide and corn cob powder.

5. A method for increasing the unsaturated fatty acid content of Landes goose liver, characterized in that, The method includes the step of feeding Landes geese a composition; the composition is the following raw materials in parts by weight: 1-9 parts of mesophilic α-1,4-amylase, 1-3 parts of pullulanase, 1-3 parts of ferulic acid esterase, 2-4 parts of glucose oxidase, 1-3 parts of catalase, 2-12 parts of a compound preparation of berberine, and 13-42 parts of a carrier; the mass ratio of the compound preparation of berberine to catalase is (2-4):1; the mass ratio of the mesophilic α-1,4-amylase to ferulic acid esterase is (1-3):1.

Images