Application of Aspergillus to Inhibiting Spontaneous Combustion of Coal
By enriching and separating Aspergillus from coal and mixing with coal powder, the coal self-ignition retardant is made, and the existing problems of short life, high cost and environmental pollution are solved, and the efficient and low-cost inhibition of coal self-ignition is achieved.
Patent Information
- Application Number
- CN202211715927.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-12-29
- Publication Date
- 2025-07-29
- Estimated Expiration
- 2042-12-29
AI Technical Summary
Existing inhibitors have short lifespans, high costs and pollute the environment in terms of inhibiting coal spontaneous combustion. Traditional methods are difficult to effectively solve the problem of coal spontaneous combustion.
Aspergillus is enriched and separated from coal and acclimates, mixed with coal powder to react, delay the oxidation process of coal powder spontaneous combustion, delay the characteristic temperature point of the spontaneous combustion, and make coal spontaneous combustion resistors.
Effectively delay the oxidation process of coal self-ignition, delay the characteristic temperature point of the self-ignition, reduce the heat release of coal, inhibit the fire of coal self-ignition, and have high resistance efficiency, low cost and environmental protection.
Smart Images
Figure CN116396790B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of coal spontaneous combustion biological inhibitors, and particularly relates to the application of Aspergillus to inhibiting coal spontaneous combustion. Background Art
[0002] Traditional inhibitors mainly inhibit coal spontaneous combustion by physical or chemical methods such as inerting, cooling, isolating, and eliminating free radicals. These inhibitors have achieved great success, but there are also some deficiencies, such as short inhibition life, with the passage of time, the inhibition effect gradually degrades, high cost, and environmental pollution after long-term use. It was previously found that microorganisms have a certain impact on the minerals and active groups in coal, and these factors are closely related to coal spontaneous combustion. Therefore, it may reduce the oxidation activity of coal. It is particularly important to extract and domestically cultivate aerobic microorganisms with the characteristics of inhibiting coal spontaneous combustion from coal.
[0003] Therefore, based on the mechanism of coal spontaneous combustion oxidation heat release, it is of great practical significance to develop a new type of inhibitor with high inhibition efficiency, low cost, low energy consumption, and environmental friendliness. Summary of the Invention
[0004] The technical problem to be solved by the present invention is to provide the application of Aspergillus to inhibiting coal spontaneous combustion in view of the deficiencies of the above-mentioned prior art. The present invention enriches and isolates an Aspergillus from a coal source and domesticates it. After mixing and reacting the domesticated Aspergillus with pulverized coal, the oxidation process of pulverized coal spontaneous combustion is delayed, the spontaneous combustion characteristic temperature point of pulverized coal is delayed, and the spontaneous combustion of pulverized coal is inhibited.
[0005] To solve the above technical problem, the technical solution adopted by the present invention is: the application of Aspergillus to inhibiting coal spontaneous combustion. After mixing and reacting the domesticated Aspergillus with pulverized coal, the oxidation process of pulverized coal spontaneous combustion is delayed, the spontaneous combustion characteristic temperature point of pulverized coal is delayed, and the spontaneous combustion of pulverized coal is inhibited.
[0006] Preferably, the domesticated Aspergillus is made into a coal spontaneous combustion inhibitor.
[0007] Preferably, the method for mixing and reacting the domesticated Aspergillus with pulverized coal is: inoculating the domesticated Aspergillus into a modified inorganic salt medium, then putting the sterilized pulverized coal and reacting under oscillation at a temperature of 28°C to 30°C for 12d to 15d, washing, filtering by suction, and drying the reacted pulverized coal to obtain a treated coal sample.
[0008] Preferably, the inoculation amount of the domesticated Aspergillus in the modified inorganic salt medium is 10%.
[0009] Preferably, the formula of the improved inorganic salt medium is KH2PO4, K2HPO4, MgSO4·7H2O, NH4NO3, NaCl, FeSO4·7H2O, ZnSO4·7H2O, CuSO4·5H2O, glucose and deionized water; the content of KH2PO4 in the improved inorganic salt medium is 1.0 g / L, the content of K2HPO4 is 1.0 g / L, the content of MgSO4·7H2O is 0.8 g / L, the content of NH4NO3 is 1.0 g / L, the content of NaCl is 0.5 g / L, the content of FeSO4·7H2O is 0.002 g / L, the content of ZnSO4·7H2O is 0.002 g / L, the content of CuSO4·5H2O is 0.002 g / L, and the content of glucose is 3.0 g / L.
[0010] Preferably, the drying temperature is 38°C to 45°C.
[0011] Preferably, the self-ignition characteristic temperature points of the pulverized coal are the critical temperature, the fissure temperature, the ignition point temperature, the maximum thermal weight loss rate temperature and the burnout temperature.
[0012] Preferably, the method for enrichment separation and domestication of the domesticated Aspergillus is as follows:
[0013] S1. Put the collected coal sample into the inorganic salt medium and enrich and culture it at a temperature of 28°C to 30°C for 48 h to obtain enriched microorganisms;
[0014] S2. Extract the enriched microorganisms obtained in S1 by the gradient dilution method. In the gradient dilution method, 9 mL of 10% NaCl solution with a concentration ratio and 1 mL of the enrichment solution are used, and dilution is carried out successively in the range of 10 -1 ~10 -7 to obtain the extracted microorganisms;
[0015] S3. Culture the extracted microorganisms obtained in S2 on the PDA solid medium by the plate streaking method at a culture temperature of 28°C to 30°C to obtain separated microorganisms:
[0016] S4. Culture the separated microorganisms obtained in S3 on the solid medium by the replacement carbon source method at a culture temperature of 28°C to 30°C to obtain the domesticated strain, and the strain is Aspergillus.
[0017] Preferably, the formula of the solid medium in S4 is 10.0 g of peptone, 5.0 g of sodium chloride, 15.0 g of pulverized coal, 15.0 g of agar and 1 L of distilled water.
[0018] The present invention has the following advantages compared with the prior art:
[0019] 1. The Aspergillus niger after domestication is reacted with pulverized coal in the present invention, which can effectively delay the oxidation process of coal spontaneous combustion, delay the characteristic temperature points of coal spontaneous combustion, reduce the heat release of coal, and inhibit the occurrence of coal spontaneous combustion fires. In addition, the domestication-modified microorganisms extracted from coal samples will not affect the environment, and can inhibit coal spontaneous combustion to the greatest extent during the growth cycle, with the advantages of high inhibition efficiency, long inhibition life, low application cost, short cultivation time, and strong reproduction ability.
[0020] 2. Compared with raw coal, for the treated coal sample obtained in the present invention, the critical temperature is delayed by 17.06 °C, the dry crack temperature is delayed by 5.15 °C, the ignition point temperature is delayed by 6.47 °C, the temperature of the maximum heat loss rate is delayed by 4.36 °C, and the burnout temperature is delayed by 33.21 °C; compared with raw coal, the heat effect of the oxidation process of the treated coal sample is reduced, the maximum heat flow value of the treated coal sample is reduced by 11.79 mW / mg, and the total heat is reduced by 1055.85 J / g.
[0021] 3. The present invention can give full play to the application of biology in the engineering field, effectively improve the international competitiveness of related fields in China, and achieve the perfect combination of biology and the coal industry.
[0022] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Description of the Drawings
[0023] Figure 1 It is a morphological picture of the microorganism in the culture medium before domestication in Example 1.
[0024] Figure 2 It is a morphological picture of the microorganism in the culture medium after domestication in Example 1.
[0025] Figure 3 It is the TG-DTG-DSC curve of the raw coal in Example 1.
[0026] Figure 4 It is the TG-DTG-DSC curve of the treated coal sample in Example 1.
[0027] Figure 5 It is the DSC-DDSC curve of the raw coal in Example 1.
[0028] Figure 6 It is the DSC-DDSC curve of the treated coal sample in Example 1. Detailed Embodiments
[0029] Example 1
[0030] Application of Aspergillus to inhibiting coal spontaneous combustion. After the domesticated Aspergillus reacts with pulverized coal, the oxidation process of pulverized coal spontaneous combustion is delayed, the spontaneous combustion characteristic temperature points of pulverized coal are postponed, and the spontaneous combustion of pulverized coal is inhibited; the spontaneous combustion characteristic temperature points of the pulverized coal are critical temperature, dry crack temperature, ignition point temperature, maximum heat loss rate temperature and burnout temperature; the domesticated Aspergillus is made into a coal flame retardant.
[0031] The method for the domesticated Aspergillus to react with pulverized coal is as follows: inoculate the domesticated Aspergillus into the improved inorganic salt medium, then put the sterilized pulverized coal and react under oscillation at a temperature of 30 °C for 12 d, wash, filter by suction and dry the reacted pulverized coal to obtain the treated coal sample; the sterilized pulverized coal has no microorganisms, and only the domesticated Aspergillus exists in the mixed reaction.
[0032] The inoculation amount of the domesticated Aspergillus in the improved inorganic salt medium is 10%; the formula of the improved inorganic salt medium is KH2PO4, K2HPO4, MgSO4·7H2O, NH4NO3, NaCl, FeSO4·7H2O, ZnSO4·7H2O, CuSO4·5H2O, glucose and distilled water; the content of KH2PO4 in the improved inorganic salt medium is 1.0 g / L, the content of K2HPO4 is 1.0 g / L, the content of MgSO4·7H2O is 0.8 g / L, the content of NH4NO3 is 1.0 g / L, the content of NaCl is 0.5 g / L, the content of FeSO4·7H2O is 0.002 g / L, the content of ZnSO4·7H2O is 0.002 g / L, the content of CuSO4·5H2O is 0.002 g / L, and the content of glucose is 3.0 g / L; the drying temperature is 38 °C.
[0033] The enrichment separation and domestication method of the domesticated Aspergillus is as follows:
[0034] S1. Put the collected coal sample into the inorganic salt medium and enrich and culture it at a temperature of 30 °C for 48 h to obtain the enriched microorganisms ( Figure 1 shown).
[0035] S2. Extract the enriched microorganisms obtained in S1 by the gradient dilution method. In the gradient dilution method, 9 mL of 10% NaCl solution with a concentration ratio and 1 mL of the enrichment solution are used, and dilution is carried out successively in the range of 10 -1 ~10 -7 to obtain the extracted microorganisms.
[0036] S3. Culture the extracted microorganisms obtained in S2 on the PDA solid medium by the plate streaking method at a culture temperature of 30 °C to obtain the separated microorganisms: the PDA solid medium is an existing medium.
[0037] S4. Culture the separated microorganisms obtained in S3 on a solid medium by the replacement carbon source method at a culture temperature of 30 °C to obtain domesticated strains ( Figure 2 as shown); the formula of the solid medium is 10.0 g of peptone, 5.0 g of sodium chloride, 15.0 g of pulverized coal, 15.0 g of agar and 1 L of distilled water;
[0038] S5. Inoculate the domesticated strains obtained in S4 into a PDA liquid medium and shake-culture for 3 d at a temperature of 30 °C. Then, perform DNA extraction, PCR amplification and molecular sequencing on the strains. Use a genomic extraction kit for genomic extraction, adopt PCR technology for molecular amplification, finally perform sequencing work, and use the sequence number for comparison. Finally, it is determined that the strain is Aspergillus, with the Latin name Aspergillus keveii, GenBank Accession number: OP164638.1.
[0039] Perform TG-DSC combined experiments on the treated coal samples and raw coal obtained in this example, analyze the thermal weight loss and thermal effect characteristics of the coal, and obtain the TG-DTG-DSC curve of the raw coal ( Figure 3 as shown), the DSC-DDSC curve of the raw coal ( Figure 5 as shown), the TG-DTG-DSC curve of the treated coal sample ( Figure 4 as shown) and the DSC-DDSC curve of the treated coal sample ( Figure 6 as shown); Figures 3 - 6 where: T1 is the critical temperature, T2 is the dry crack temperature, T3 is the ignition point temperature, T4 is the maximum thermal weight loss rate temperature, and T5 is the burnout temperature.
[0040] From Figure 3 and Figure 4 it can be seen that after treatment, the heat and heat flow of the coal sample are both reduced, indicating that the thermal effect of the treated coal sample is weakened; compared with the raw coal, for the treated coal sample, the critical temperature is delayed by 17.06 °C, the dry crack temperature is delayed by 5.15 °C, the ignition point temperature is delayed by 6.47 °C, the maximum thermal weight loss rate temperature is delayed by 4.36 °C, and the burnout temperature is delayed by 33.21 °C. From Figure 5 and Figure 6 it can be known that compared with the raw coal, the maximum heat flow value of the treated coal sample is reduced by 11.79 mW / mg, and the total heat is reduced by 1055.85 J / g, indicating that the coal sample after mixing and reacting with the domesticated Aspergillus can effectively reduce the thermal effect during the oxidation process, thus effectively delaying the oxidation process of coal spontaneous combustion, delaying the characteristic temperature points of coal spontaneous combustion, reducing the heat release of coal, and inhibiting the occurrence of coal spontaneous combustion fires.
[0041] Example 2
[0042] Application of Aspergillus to Inhibiting Coal Spontaneous Combustion: After the domesticated Aspergillus reacts with pulverized coal, the oxidation process of the spontaneous combustion of pulverized coal is delayed, the characteristic temperature points of the spontaneous combustion of pulverized coal are postponed, and the spontaneous combustion of pulverized coal is inhibited; the characteristic temperature points of the spontaneous combustion of the pulverized coal are critical temperature, dry crack temperature, ignition point temperature, maximum heat loss rate temperature and burnout temperature; the domesticated Aspergillus is made into a coal flame retardant;
[0043] The method for the domesticated Aspergillus to react with pulverized coal is as follows: inoculate the domesticated Aspergillus into a modified inorganic salt medium, then put the sterilized pulverized coal and react with shaking at a temperature of 28°C for 15 days, wash, filter and dry the reacted pulverized coal to obtain the treated coal sample; the sterilized pulverized coal has no microorganisms, and only the domesticated Aspergillus exists in the mixed reaction;
[0044] The inoculation amount of the domesticated Aspergillus in the modified inorganic salt medium is 10%; the formula of the modified inorganic salt medium is KH2PO4, K2HPO4, MgSO4·7H2O, NH4NO3, NaCl, FeSO4·7H2O, ZnSO4·7H2O, CuSO4·5H2O, glucose and distilled water; the content of KH2PO4 in the modified inorganic salt medium is 1.0 g / L, the content of K2HPO4 is 1.0 g / L, the content of MgSO4·7H2O is 0.8 g / L, the content of NH4NO3 is 1.0 g / L, the content of NaCl is 0.5 g / L, the content of FeSO4·7H2O is 0.002 g / L, the content of ZnSO4·7H2O is 0.002 g / L, the content of CuSO4·5H2O is 0.002 g / L, and the content of glucose is 3.0 g / L; the drying temperature is 45°C.
[0045] The enrichment separation and domestication method of the domesticated Aspergillus is as follows:
[0046] S1. Put the collected coal sample into an inorganic salt medium and enrich and culture it at a temperature of 28°C for 48 h to obtain enriched microorganisms;
[0047] S2. Extract the enriched microorganisms obtained in S1 by the gradient dilution method. In the gradient dilution method, 9 mL of a 10% NaCl solution and 1 mL of the enrichment solution are used, and dilution is carried out in sequence in the range of 10 -1 ~10 -7 to obtain the extracted microorganisms;
[0048] S3. Culture the extracted microorganisms obtained in S2 on a PDA solid medium by the plate streaking method at a culture temperature of 28°C to obtain separated microorganisms: the PDA solid medium is an existing medium;
[0049] S4. Cultivate the isolated microorganisms obtained in S3 on a solid medium using the replacement carbon source method at a cultivation temperature of 28 °C to obtain domesticated strains; the formula of the solid medium is 10.0 g of peptone, 5.0 g of sodium chloride, 15.0 g of coal powder, 15.0 g of agar and 1 L of distilled water;
[0050] S5. Inoculate the domesticated strains obtained in S4 into a PDA liquid medium and shake-cultivate for 3 d at a temperature of 30 °C, then perform DNA extraction, PCR amplification and molecular sequencing on the strains. Use a genomic extraction kit for genomic extraction, adopt PCR technology for molecular amplification, finally perform sequencing work, and use the sequence number for comparison. Finally, it is determined that the strain is Aspergillus, with the Latin name Aspergillus keveii, GenBank Accession number: OP164638.1.
[0051] In this example, the domesticated Aspergillus is reacted with coal powder, which can effectively delay the oxidation process of coal spontaneous combustion, delay the characteristic temperature points of coal spontaneous combustion, reduce the heat release of coal, and inhibit the occurrence of coal spontaneous combustion fires.
[0052] Example 3
[0053] The application of Aspergillus provided in this example in inhibiting coal spontaneous combustion. After the domesticated Aspergillus is mixed and reacted with coal powder, it delays the oxidation process of coal powder spontaneous combustion, delays the characteristic temperature points of coal powder spontaneous combustion, and inhibits the spontaneous combustion of coal powder; the characteristic temperature points of coal powder spontaneous combustion are critical temperature, cracking temperature, ignition point temperature, maximum thermal weight loss rate temperature and burnout temperature; the domesticated Aspergillus is made into a coal flame retardant;
[0054] The method for mixing and reacting the domesticated Aspergillus with coal powder is as follows: inoculate the domesticated Aspergillus into a modified inorganic salt medium, then put the sterilized coal powder and react under shaking at a temperature of 29 °C for 14 d. Wash, filter and dry the reacted coal powder to obtain a treated coal sample; the sterilized coal powder has no microorganisms, and only the domesticated Aspergillus exists in the mixing reaction;
[0055] The inoculation amount of the domesticated Aspergillus in the improved inorganic salt medium is 10%; the formula of the improved inorganic salt medium is KH2PO4, K2HPO4, MgSO4·7H2O, NH4NO3, NaCl, FeSO4·7H2O, ZnSO4·7H2O, CuSO4·5H2O, glucose and distilled water; the content of KH2PO4 in the improved inorganic salt medium is 1.0 g / L, the content of K2HPO4 is 1.0 g / L, the content of MgSO4·7H2O is 0.8 g / L, the content of NH4NO3 is 1.0 g / L, the content of NaCl is 0.5 g / L, the content of FeSO4·7H2O is 0.002 g / L, the content of ZnSO4·7H2O is 0.002 g / L, the content of CuSO4·5H2O is 0.002 g / L, and the content of glucose is 3.0 g / L; the drying temperature is 42 °C.
[0056] The enrichment separation and domestication method of the domesticated Aspergillus is as follows:
[0057] S1. Put the collected coal sample into the inorganic salt medium and enrich and culture it at 29 °C for 48 h to obtain enriched microorganisms;
[0058] S2. Extract the enriched microorganisms obtained in S1 by the gradient dilution method. In the gradient dilution method, 9 mL of 10% NaCl solution with a concentration ratio and 1 mL of the enrichment solution are used, and dilution is carried out in sequence in the range of 10 -1 ~10 -7 to obtain the extracted microorganisms;
[0059] S3. Culture the extracted microorganisms obtained in S2 by the plate streaking method on the PDA solid medium at a culture temperature of 29 °C to obtain separated microorganisms: the PDA solid medium is an existing medium;
[0060] S4. Culture the separated microorganisms obtained in S3 by the carbon source replacement method on the solid medium at a culture temperature of 29 °C to obtain domesticated strains; the formula of the solid medium is 10.0 g of peptone, 5.0 g of sodium chloride, 15.0 g of coal powder, 15.0 g of agar and 1 L of distilled water;
[0061] S5. Inoculate the domesticated strain obtained in S4 into a PDA liquid medium, shake and culture it at a temperature of 30 °C for 3 days, then perform DNA extraction, PCR amplification and molecular sequencing on the strain. Use a genomic extraction kit for genomic extraction, adopt PCR technology for molecular amplification, finally perform sequencing work, and use the sequence number for comparison. Finally, it is determined that the strain is Aspergillus, with the Latin name Aspergillus keveii, GenBank Accession number: OP164638.1.
[0062] In this embodiment, the reaction of the domesticated Aspergillus with pulverized coal can effectively delay the oxidation process of coal spontaneous combustion, delay the characteristic temperature point of coal spontaneous combustion, reduce the heat release of coal, and inhibit the occurrence of coal spontaneous combustion fires.
[0063] The above are only the preferred embodiments of the present invention and do not impose any limitations on the present invention. Any simple modifications, changes and equivalent changes made to the above embodiments according to the technical essence of the invention still fall within the protection scope of the technical solution of the present invention.
Claims
1. Application of Aspergillus to inhibiting coal spontaneous combustion, characterized in that, The domesticated strain is obtained by enrichment, extraction, separation and domestication from the collected coal samples, and it is determined by sequencing and identification that the domesticated strain is Aspergillus Aspergillus keveii ; After the domesticated Aspergillus is mixed and reacted with pulverized coal, the oxidation process of the spontaneous combustion of pulverized coal is delayed, the characteristic temperature point of the spontaneous combustion of pulverized coal is delayed, and the spontaneous combustion of pulverized coal is inhibited.
2. The application of Aspergillus to inhibiting coal spontaneous combustion according to claim 1, characterized in that, Make the domesticated Aspergillus into an inhibitor for coal spontaneous combustion.
3. Use of Aspergillus for inhibiting spontaneous combustion of coal according to claim 1, characterized in that, The method for the mixed reaction of the domesticated Aspergillus and pulverized coal is as follows: inoculate the domesticated Aspergillus into a modified inorganic salt medium, then put the sterilized pulverized coal into it and react under oscillation at a temperature of 28°C to 30°C for 12d to 15d, and then wash, filter by suction and dry the reacted pulverized coal to obtain the treated coal sample.
4. The application of Aspergillus to inhibiting coal spontaneous combustion according to claim 3, characterized in that The inoculation amount of the domesticated Aspergillus in the modified inorganic salt medium is 10%.
5. The application of Aspergillus to inhibiting coal spontaneous combustion according to claim 4, characterized in that, The formula of the modified inorganic salt medium is KH2PO4, K2HPO4, MgSO4·7H2O, NH4NO3, NaCl, FeSO4·7H2O, ZnSO4·7H2O, CuSO4·5H2O, glucose and distilled water; the content of KH2PO4 in the modified inorganic salt medium is 1.0g / L, the content of K2HPO4 is 1.0g / L, the content of MgSO4·7H2O is 0.8g / L, the content of NH4NO3 is 1.0g / L, the content of NaCl is 0.5g / L, the content of FeSO4·7H2O is 0.002g / L, the content of ZnSO4·7H2O is 0.002g / L, the content of CuSO4·5H2O is 0.002g / L, and the content of glucose is 3.0g / L.
6. The application of Aspergillus to inhibiting coal spontaneous combustion according to claim 3, characterized in that The temperature of the drying is 38°C to 45°C.
7. The application of Aspergillus to inhibiting coal spontaneous combustion according to claim 1, wherein The spontaneous combustion characteristic temperature points of the pulverized coal are the critical temperature, the dry crack temperature, the ignition point temperature, the maximum heat loss rate temperature and the burnout temperature.
8. Use of Aspergillus for inhibiting spontaneous combustion of coal according to claim 1, characterized in that, The enrichment separation and domestication method of the domesticated Aspergillus is as follows: S1. Put the collected coal sample into an inorganic salt medium and enrich and culture it at a temperature of 28°C to 30°C for 48h to obtain enriched microorganisms. S2. Extract the enriched microorganisms obtained in S1 by the gradient dilution method. In the gradient dilution method, use 9 mL of 10% NaCl solution with a concentration ratio and 1 mL of the enrichment solution, and sequentially dilute from 10 -1 ~10 -7 range to obtain the extracted microorganisms; S3. Culture the extracted microorganisms obtained in S2 by the plate streaking method on a PDA solid medium at a temperature of 28°C to 30°C to obtain separated microorganisms. S4. Culture the separated microorganisms obtained in S3 by the carbon source replacement method on a solid medium at a temperature of 28°C to 30°C to obtain the domesticated strain, and the strain is Aspergillus.
9. The application of Aspergillus in inhibiting coal spontaneous combustion according to claim 8, characterized in that, The formula of the solid medium in S4 is 10.0g peptone, 5.0g sodium chloride, 15.0g pulverized coal, 15.0g agar and 1L distilled water.