A skin care composition for repairing skin barrier and its application

Abstract

The present invention provides a skin care composition for repairing the skin barrier and its use. The skin care composition comprises a combination of active ingredients for repairing the skin barrier, comprising a bifid yeast fermentation lysate, sialic acid, and a pH adjuster, wherein the pH adjuster is used in an amount such that the pH of the resulting skin care composition is no less than 4.5. Compared to using bifid yeast fermentation lysate alone, the combination of bifid yeast fermentation lysate and sialic acid in the present invention can enhance the efficacy of skin barrier repair.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a skin care composition for repairing skin barrier and application thereof. Background Art

[0002] A defective skin barrier allows more water to be lost from the skin and external irritants (such as allergens and toxins) to invade, triggering dryness, redness, itching, etc. Various factors can cause the skin to fail to successfully complete its barrier function, such as skin damage caused by ultraviolet rays, or as people age, the skin's ability to regenerate its barrier function after being stimulated becomes increasingly problematic.

[0003] The skin's barrier function is primarily manifested through the stratum corneum, the outermost layer of the skin. Composed of necrotic corneocytes and intercellular lipids, the stratum corneum serves as a protective membrane, protecting the skin from external stimuli and preventing water evaporation from the interior. This core function is the skin's protective layer. Furthermore, the stratum corneum not only performs a barrier function but also influences the function, function, and structure of the underlying cell layers, namely the epidermis and dermis.

[0004] Bifid yeast ferment lysate, also known as Bifidobacterium bifidum ferment lysate, is a comprehensive collection of metabolites, cytoplasmic fragments, cell wall components, and polysaccharide complexes obtained through the cultivation, inactivation, and decomposition of bifidobacteria. These products include B vitamins, minerals, amino acids, and other beneficial small molecules, making them a highly suitable yeast extract for skincare. Bifid yeast ferment lysate can enhance stratum corneum metabolism, exhibits strong anti-immunosuppressive activity, and promotes DNA repair, effectively protecting the skin from UV damage. It boosts skin immunity and repairs the skin barrier.

[0005] However, due to factors such as raw material stability, it is often difficult to obtain raw materials with high effective content from the lysate of bifid yeast fermentation products. The amount added during application is high, and the economic cost cannot be ignored. Summary of the Invention

[0006] The object of the present invention is to provide a skin care composition that can repair the skin barrier and thus improve the skin's moisturizing ability.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] A skin care composition comprises an active ingredient combination for repairing the skin barrier, wherein the active ingredient combination comprises a bifid yeast fermentation product lysate, sialic acid, and a pH regulator, wherein the amount of the pH regulator is such that the pH of the obtained skin care composition is not less than 4.5.

[0009] As a known technology, the lysate of bifid yeast fermentation product is also called bifid yeast fermentation product lysate, and in some cases it can be simply called bifid yeast. It contains small molecules that are beneficial to skin care, such as vitamin B group, minerals, amino acids, etc., which can enhance the metabolism of the stratum corneum, has strong anti-immunosuppressive activity and can promote DNA repair, thereby enhancing skin immunity and repairing the skin barrier.

[0010] The present invention has found that compared with the use of a bifid yeast fermentation product lysate as an active ingredient for repairing the skin barrier, the bifid yeast fermentation product lysate is compounded with sialic acid and used together. The resulting active ingredient combination can significantly improve the efficacy of repairing the skin barrier. However, both the bifid yeast fermentation product lysate and sialic acid are acidic substances. Direct use can cause skin damage to normal skin, especially people with a thin stratum corneum. In order to avoid causing skin problems such as redness and inflammation, as well as unpleasant skin sensations such as stinging and burning, the present invention also requires the addition of a pH regulator to balance the skin's use experience. The sialic acid described in the present invention is also known as N-acetylneuraminic acid, bird's nest acid, and bird's nest extract.

[0011] The pH adjusters described herein are primarily alkaline substances, such as arginine, betaine, and triethanolamine, which are commonly used in cosmetics. These substances can achieve the objectives of the present invention, and their usage is not particularly limited. However, one embodiment of the present invention demonstrates that arginine, when used as the alkaline substance, exhibits superior effects compared to other pH adjusters.

[0012] In a preferred embodiment, the mass ratio of the bifid yeast fermentation product lysate to sialic acid is 1:(0.1-3).

[0013] In a further preferred embodiment, the mass ratio of the bifid yeast fermentation product lysate to sialic acid is 1:(0.6-1.5).

[0014] In the most preferred embodiment, the mass ratio of the bifid yeast fermentation product lysate to sialic acid is 1:(0.8-1.2).

[0015] The present invention has found through experiments that, within the above-mentioned ratio range, the lysate of the bifid yeast fermentation product and sialic acid can more significantly improve the efficacy of repairing the skin barrier.

[0016] Since most of the currently available bifid yeast fermentation product lysates are aqueous solutions or mixtures in other forms, the mass or weight content of the bifid yeast fermentation product lysate in the present invention can be calculated according to the effective content indicated therein.

[0017] In a preferred embodiment, the skin care composition comprises:

[0018] 0.1 to 20% by weight of the active ingredient combination;

[0019] The weight % herein are each relative to the total skin care composition.

[0020] In the skin care composition, the conventional amount of the active ingredient combination for repairing the skin barrier is above 0.1 wt %. When the amount of the active ingredient combination exceeds 20 wt %, it may cause harmful irritation side effects to the human body, and is therefore not preferred.

[0021] In a preferred embodiment, the skin barrier repair feature of the aforementioned skin care composition is reduced water loss and enhanced moisture retention. The stratum corneum, the skin's primary defense membrane, is not only susceptible to damage from the external environment, but also, in individuals with dry skin, the stratum corneum's barrier function is impaired or even compromised. To provide moisture retention for dry skin, moisturizers are often used as a temporary solution, but this rarely addresses the underlying problem. Furthermore, UV-damaged skin barriers cannot be improved simply by applying moisturizers.

[0022] In another preferred embodiment, in the above skin care composition, the feature of repairing the skin barrier is to inhibit skin damage caused by ultraviolet rays.

[0023] The inhibition of skin damage caused by ultraviolet rays described in the present invention should be understood as the role of the active ingredient combination in preventing further deterioration, damage, and degeneration of damaged or impending skin, and repairing existing light damage during the irradiation process containing ultraviolet rays; and the meaning of inhibition can be synonymous with slowing down, alleviating, relieving, repairing, repairing, and treating; skin damage refers to the phenomena such as increased dandruff, peeling, local hardening, roughness, thickening, and dryness that appear on the skin surface under the toxic effect of ultraviolet light, or the pathological phenomena such as swelling and enlargement of various cells in the epidermis and dermis of the skin.

[0024] Furthermore, the inhibition of skin damage caused by ultraviolet rays is to slow down abnormal keratin proliferation in photo-aged skin; or, inhibit thickening of photo-aged skin; or, reduce the malondialdehyde content in the blood.

[0025] The mitigation and repair mentioned in the present invention should be understood as the active ingredients combining to restore softness and reduce dandruff when the damaged skin is exposed to excessive light or daily light, such as dandruff, dryness, hardening and other minor or serious damage. The meaning of mitigation or repair can be synonymous with alleviating, treating, relieving, inhibiting, repairing, etc.

[0026] Abnormal keratinosis of the skin described in the present invention refers to the phenomenon of obvious thickening of the epidermis, enlargement of keratinocytes, and excessive keratinization of the skin after phototoxicity.

[0027] The malondialdehyde content measured in the present invention can reflect the degree of lipid peroxidation in the body based on the content characteristics of malondialdehyde. The level of malondialdehyde can be used as one of the indicators to examine the severity of cell stress.

[0028] In a preferred embodiment, the skin care composition is in the form of a facial cleanser, essence, lotion, cream, ointment, facial mask, cosmetic nourishing lotion, or foundation. Furthermore, as needed, the composition may appropriately contain components commonly used in cosmetic products or external skin preparations, such as moisturizers, antioxidants, surfactants, oily components, UV absorbers, emulsifiers, thickeners, aqueous components, water, and various skin-applicable ingredients, without impairing the effects of the present invention.

[0029] In the present invention, unless otherwise specified:

[0030] As used herein, "comprising" is intended to mean "including but not necessarily consisting of..." In other words, the listed options are not necessarily exhaustive.

[0031] The "skin care composition" used in the present invention is intended to include compositions for topical application to the skin of mammals, especially humans. Such compositions can generally be classified as leave-on or rinse-off, preferably leave-on.

[0032] As used herein, "skin" is meant to include skin on the face and body (eg, neck, chest, back, arms, hands, legs, etc.), preferably facial skin.

[0033] The beneficial effects achieved by the present invention are:

[0034] The present invention provides a skin care composition, which contains an active ingredient combination for repairing the skin barrier. The active ingredient combination is a compound of a bifid yeast fermentation product lysate, sialic acid, and a pH regulator, which can improve the efficacy of repairing the skin barrier while reducing the risk of irritation to the skin. DETAILED DESCRIPTION

[0035] The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention. Where specific techniques or conditions are not specified in the examples, the techniques or conditions described in the literature in the art or the product instructions were used.

[0036] In the following examples and comparative examples, the bifid yeast fermentation product lysate was sourced from Synsil (USA), with an effective content of 25%; and the sialic acid was commercially available from the applicant (content 98%). The mass components of the bifid yeast fermentation product lysate in the examples were calculated based on their labeled effective contents.

[0037] The final pH of all examples was adjusted to 5.5 ± 0.2.

[0038] Example 1

[0039] This embodiment provides a skin care composition, consisting of the following components:

[0040]

[0041] Example 2

[0042] This embodiment provides a skin care composition, consisting of the following components:

[0043]

[0044] Example 3

[0045] This embodiment provides a skin care composition, consisting of the following components:

[0046]

[0047] Example 4

[0048] This embodiment provides a skin care composition, consisting of the following components:

[0049]

[0050] Comparative Example 1

[0051] This comparative example provides a skin care composition consisting of the following components:

[0052]

[0053] Comparative Example 2

[0054] This comparative example provides a skin care composition consisting of the following components:

[0055]

[0056] Comparative Example 3

[0057] This comparative example provides a skin care composition consisting of the following components:

[0058]

[0059] Test Example 1 Irritation Test

[0060] This test example tests the irritation and skin feel of the skin care compositions provided in Example 1 and Comparative Examples 1-2 when applied to the skin, as follows:

[0061] Fifteen non-pregnant women aged between 20 and 40 were selected as subjects and randomly divided into three groups, with five women in each group. The skin care compositions provided in Example 1, Comparative Example 1, and Comparative Example 2 were used respectively.

[0062] Test method: Refer to the "Technical Specifications for Safety of Cosmetics (2015 Edition)" and conduct the "Human Skin Patch Test" on the product. Use a skin occlusive patch test method for 0.5 hours. Remove the patch tester 0.5 hours later. Determine the irritation according to Table 1. The total score is the sum of individual scores divided by the number of people.

[0063] Table 1 Skin reaction grading standards for skin occlusive patch test

[0064]

[0065] Test results: Example 1 scored 0, Comparative Example 1 scored 0.2, and Comparative Example 2 scored 0. In addition, 3 subjects in Comparative Example 1 expressed obvious irritation. Based on the above, Comparative Example 1 is not suitable for further experiments.

[0066] Test Example 2: Repairing the Skin Barrier

[0067] This test example tests the skin barrier repair function of the skin care compositions provided in Examples 1-4 and Comparative Example 2, as follows:

[0068] The test month was September, and 25 non-pregnant women aged between 25 and 40 were selected as subjects and randomly divided into 5 groups, with 5 people in each group. They used the skin care compositions provided by Examples 1-4 and Comparative Example 2 respectively. Since no other stabilizer ingredients were added, the compositions were prepared daily for use in the evening of the same day and the morning of the next day.

[0069] Test method: After washing in the morning and evening, apply 0.5 mL of the skin care composition to the cheek for 4 weeks. Do not use other skin care products on the test area during the test period.

[0070] Testing Method: Using the TM300 from German company CK, the transepidermal water loss (TEWL) of the cheek was measured at week 0, week 2, and week 4, and the average value (rounded to one decimal place) was taken. The test results are shown in Table 2.

[0071] Table 2 TEWL test results

[0072] <![CDATA[Week 0 g / m 2 h]]> <![CDATA[Week 2 g / m 2 h]]> <![CDATA[Week 4 g / m 2 h]]> Example 1 19.1 16.4 15.8 Example 2 19.3 17.0 16.5 Example 3 18.9 16.8 16.2 Example 4 19.1 17.2 16.9 Comparative Example 2 18.8 17.4 17.0

[0073] Test Example 3 UV repair test

[0074] This example studies the ability of the skin care compositions provided in Example 1 and Comparative Examples 2-3 to inhibit skin aging caused by ultraviolet rays. The specific process is as follows:

[0075] 1. Test materials

[0076] 1.1 Test samples

[0077] The skin care compositions provided in Example 1 and Comparative Examples 2-3.

[0078] 1.2 Blank control

[0079] The blank control was purified water.

[0080] 1.3 Experimental animals

[0081] The experimental ICR mice, SPF grade, female, weighing 18-22 g, were provided by Wuhan Institute of Biological Products Co., Ltd.

[0082] Animal husbandry: The experimental animals were kept in cages, with 6 to 8 animals per cage. The feed was SPF-grade experimental granular mouse food, which can be purchased commercially. The drinking water was sterilized purified water. The cage bottom bedding was poplar wood chips, which were sterilized before use.

[0083] The medium was changed every two days. The laboratory temperature was 20-22°C, the humidity was 55-60%, and the light-dark cycle was 12 h.

[0084] 1.4 Reagents

[0085] Methoxsalen (8-methoxypsoralen), 1 g / bottle, SLBC0610V, purchased from Sigma-Aldrich. Dissolve in 95% ethanol and dilute to 1% concentration before use. Protect from light.

[0086] Superoxide dismutase (SOD) kit (WST-1 method): purchased commercially.

[0087] Malondialdehyde (MDA) kit: purchased commercially.

[0088] 1.5 Instruments

[0089] The HOPE-MED 8130 skin phototoxicity tester, UV-A irradiator, TU1950 UV spectrophotometer, INFINITE M200 fluorescence multifunctional microplate reader, ASP200S automatic tissue dehydrator, EG1150 paraffin embedding machine, HI1210 slide spreader, RM2265 automatic slicer, and DM2500 microphotographer used are all commercially available.

[0090] 2. Test methods

[0091] 2.1 Modeling method

[0092] Fifty female ICR mice were obtained and fed adaptively for one week. The back hair of the mice was removed using rosin paraffin wax plucking, covering an area of ​​2 cm × 3 cm. Forty mice were selected as the model group after depilation. 0.1 ml of methoxsalen solution was applied to the exposed area of ​​the back skin. One hour later, the mice were fixed in a transparent mouse holder and placed in a skin phototoxicity tester. The mice were irradiated approximately 15 cm from the light source (UV-A energy measured by an ultraviolet irradiator was 2.25 mW / cm). 2 ); another 10 mice served as a normal control group and were not irradiated or applied with methoxsalen solution. Modeling group mice were irradiated once daily for 15 minutes, one hour after application of methoxsalen solution, for two consecutive weeks. Thereafter, they were irradiated once daily for 15 minutes, without methoxsalen application, for three consecutive weeks. All mice were shaved every two days with an animal shaver to remove all exposed hair from their backs.

[0093] 2.2 Animal grouping and drug administration

[0094] All 40 mice were successfully modeled (randomly divided into 3 groups according to the degree of light damage to the back skin: Example 1 group (10 mice), Comparative Example 2 group (10 mice), Comparative Example 3 group (10 mice) and Model Control Group (10 mice).

[0095] In Example 1 and Comparative Examples 2-3, 0.2 mL of the skin care composition was diluted 10-fold and applied to the mice. The model control group was given the same volume of purified water. After 30 minutes, the mice were secured in a transparent mouse holder and placed in a skin phototoxicity tester. The mice were irradiated at a distance of approximately 15 cm from the light source for 15 minutes per session, once daily. This procedure was repeated for 5 consecutive weeks. All mice were shaved every two days using an animal shaver to remove all hair from the exposed back skin. The normal control group was not irradiated.

[0096] 2.3 Experimental observation and sampling

[0097] During the experiment, the smoothness, color, softness, and elasticity of the skin of mice in each group were observed visually. Twenty-four hours after the final irradiation, approximately 1.0 ml of blood was collected from the orbital venous plexus of mice in the treatment and model groups. The blood was centrifuged at 3000 rpm for 10 minutes to obtain serum. The mice were then sacrificed by cervical dislocation. Full-thickness skin from the depilated area on the back was obtained and fixed in 10% neutral formalin for histopathological section preparation. Serum was used to measure MDA content and SOD activity. Mice in the normal control group were treated in the same manner.

[0098] 3. Test results

[0099] 3.1 Visual observation during the test

[0100] The back skin of mice in the normal control group was smooth, delicate and elastic; the skin of mice in the model control group gradually became dandruff, dry, and partially peeled as the irradiation time increased, and the skin color became dark, locally hardened, rough and thickened; after the mice in the test sample group were irradiated and the drug was applied at the same time, the skin damage was alleviated to varying degrees, with the color becoming lighter and the dandruff reducing.

[0101] 3.2 Effect of sialic acid on serum MDA content in photoaged mice

[0102] Table 3 Effect of bird's nest acid on MDA content in photoaging mouse model

[0103] Group Number of animals (n) MDA content (nmol / l) Normal control group 10 21.16±2.26 Model control group 10 <![CDATA[28.21±6.02 △△ ]]> Example 1 group 10 22.83±4.04** Comparative Example 2 10 25.85±2.68** Comparative Example 3 10 24.15±2.35**

[0104] Note: △△ indicates p < 0.01 compared with the normal control group; ** indicates p < 0.01 compared with the model control group.

[0105] The results in Table 3 show that the MDA content in the serum of each group of mice was significantly higher in the model control group compared with the normal control group, indicating that the mouse skin photoaging model was successfully established.

[0106] Compared with the model control group, the three groups of samples could significantly reduce the content of MDA in serum, which was statistically significant, but the effect of the group supplemented with bifid yeast fermentation product lysate and sialic acid was more obvious.

[0107] 3.3 Pathological tissue section examination

[0108] For the histopathological sections of the skin of mice in each experimental group, 5 fields of view were selected for each tissue, and the thickness of the epidermis and dermis was measured under a microscope using the LASV4.0 software provided with the microscope. The statistical calculations were performed. The results are shown in Table 4:

[0109] Table 4 Effects of bird's nest acid on the thickness of epidermis and dermis in photoaging mouse model

[0110]

[0111] Note: △△ indicates p < 0.01 compared with the normal control group; ** indicates p < 0.01 compared with the model control group.

[0112] The three sample groups showed less epidermal thickening and keratinization, and dermal fibrosis and thickening than the model control group, but the effect of Example 1 was more obvious.

[0113] Although the present invention has been described in detail above using general explanations, specific embodiments, and experiments, it will be apparent to those skilled in the art that modifications and improvements may be made based on the present invention. Therefore, such modifications and improvements, which do not depart from the spirit of the present invention, are intended to be within the scope of protection claimed herein.

Claims

1. An application of an active ingredient combination comprising a bifid yeast fermentation product lysate, sialic acid, and a pH regulator in preparing a skin care composition for repairing the skin barrier, characterized in that: The amount of the pH regulator is such that the pH of the obtained skin care composition is not less than 4.5, and the mass ratio of the bifid yeast fermentation product lysate to sialic acid is 1:(0.1-3); The pH regulator is arginine; The characteristic of repairing the skin barrier is to inhibit skin damage caused by ultraviolet rays; The inhibition of skin damage caused by ultraviolet rays is to slow down abnormal keratin proliferation of the skin; and / or to inhibit the thickening of the skin caused by photoaging.

2. The use according to claim 1, characterized in that The mass ratio of the bifid yeast fermentation product lysate to sialic acid is 1:(0.6-1.5).

3. The use according to claim 1, characterized in that The mass ratio of the bifid yeast fermentation product lysate to sialic acid is 1:(0.8-1.2).

4. The use according to any one of claims 1 to 3, characterized in that The skin care composition comprises: 0.1 to 20% by weight of the active ingredient combination; The weight % is relative to the total skin care composition.

5. The use according to any one of claims 1 to 3, characterized in that The characteristics of repairing the skin barrier also include reducing water loss from the skin and improving the skin's moisturizing ability.

6. The use according to any one of claims 1 to 3, characterized in that The skin care composition is in the form of a facial cleanser, essence, lotion, cream, ointment, facial mask, cosmetic nourishing water or foundation.

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