An automated pipetting device and method of use thereof

By designing an automatic pipetting device, which utilizes a main air pump, a one-way valve, and a conductive circuit to control liquid intake and dispensing, the problems of low accuracy and complex operation of existing pipettes are solved, achieving high-precision, easy-to-operate, and stable and efficient pipetting results.

CN116440973BActive Publication Date: 2026-02-27WUHU SHIPYARD CO LTD +1
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Patent Information

Application Number
CN202310421173.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-04-19
Publication Date
2026-02-27
Estimated Expiration
2043-04-19

AI Technical Summary

Technical Problem

Existing pipettes are not very accurate and are complicated to operate. The use of rubber bulbs is difficult and manual operation can easily lead to liquid leakage, which affects the accuracy and efficiency of experimental results.

Method used

The automatic pipetting device is composed of a main air pump, a main check valve, a venting branch pipe, an electrically controlled valve, a suction branch pipe, an auxiliary air pump, an auxiliary check valve, a conductive anode, and a cathode. The automatic control system precisely controls the intake and dripping of liquid through a conductive circuit and a control module, and the pipetting tube is fixed by an adjustable bracket and clamp.

Benefits of technology

It achieves high precision and simple operation, avoids liquid leakage, improves working stability and efficiency, and is easy to clean and maintain.

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Abstract

The application belongs to the automatic pipetting device in the technical field of automatic pipetting, and also relates to a use method of the automatic pipetting device. A main air pump (2) and a main one-way valve (7) are arranged at the top of a pipetting tube body (1), and the main air pump (2) is located above the main one-way valve (7). A ventilation branch pipe (3) is arranged on the upper part of the pipetting tube body (1) and communicates with the pipetting tube body (1), and the ventilation branch pipe (3) is located below the main one-way valve (7). An electric control valve (4) is arranged at the end of the ventilation branch pipe (3). An air extraction branch pipe (5) is arranged on the upper part of the pipetting tube body (1) and communicates with the pipetting tube body (1), and the air extraction branch pipe (5) is located below the main one-way valve (7). The automatic pipetting device can solve the problems in the prior art, avoid the defect of liquid outflow caused by excessive vibration in the manual operation process, and has the advantages of high accuracy, simple operation, reliable use, stable work, high efficiency, easy cleaning and convenient maintenance.
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Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of automatic pipetting, and more particularly to an automatic pipetting device and a method for using the same. BACKGROUND

[0002] A pipette is a device used to accurately measure and transfer a specific volume of liquid. In the process of material chemical analysis test, it is often necessary to use a pipette to move the solution. The existing pipette must be used in cooperation with a bulb syringe. The solution is sucked into the pipette by squeezing the bulb syringe. The operation steps are as follows: the lower end of the pipette is placed below the liquid surface of the solution to be sucked, the bulb syringe is tightly attached to the upper end of the pipette, the air in the bulb syringe is discharged, the bulb syringe is slowly released, the solution to be sucked is entered into the pipette, the bulb syringe is removed when the pipette is filled with liquid, the upper end of the pipette is blocked with a finger, the pipette is moved to above the container to be dripped, the finger blocking the upper end of the pipette is slightly loosened, the liquid surface in the pipette will drop, and the pipetting operation is completed after the solution flows out.

[0003] The main defects of the prior art pipette are as follows: the precision of the pipette is low. It is difficult to control the degree of squeezing the bulb syringe. If the squeezing force is too large, the solution sucked into the pipette will overflow into the bulb syringe, causing contamination of the solution and damage to the bulb syringe. If the squeezing force is not enough, the solution cannot reach the scale line and needs to be re-operated. The bulb syringe and the pipette are usually stored separately, and the opening of the bulb syringe is exposed to the air, which is easy to be contaminated with dust, which will affect the experimental results when used again. Most pipettes are blocked at the upper end by a finger after washing, and the liquid level in the pipette is adjusted by pressing the upper end of the pipette with the finger, which makes it difficult to accurately reach the scale line. When sucking easily volatile and corrosive liquids such as concentrated hydrochloric acid, there is a safety problem in blocking the upper end of the pipette with a finger. During the manual operation of the pipette, the liquid in the pipette is easily spilled due to excessive movement of the operator, which increases the difficulty of pipetting operation, reduces work efficiency, and affects the accuracy of the test results.

[0004] There is a technology named "automatic quantitative pipette" with a publication number "2178887Y" in the prior art. The technology has a pipe sleeve 2 made of transparent material, a pipe sleeve head 6 fixed on the hollow cylindrical section of the pipe sleeve 2, a pipe body 1 slidably and tightly connected in the pipe sleeve 2, a pipe body head 7 fixed at the top end of the pipe body 1, a limit lever 4 in the shape of inverted T passing through the edge hole of the pipe sleeve head 6 and the edge hole of the pipe body head 7, nuts 9 and 8 fixed on the upper and lower holes of the pipe body head 7, and a spring 3 surrounding the outer periphery of the pipe sleeve 2 and the pipe body 1 between the pipe sleeve head 6 and the pipe body head 7. The technology does not involve the technical problems and solutions of the present application. SUMMARY

[0005] The technical problem to be solved by the present application is to provide an automatic pipetting device which can solve the problems of low precision, complex operation and large operation difficulty of the existing ear bulb, avoid the defect of liquid outflow caused by excessive vibration in the manual operation process, and has high precision, simple operation, stable work, high efficiency, easy cleaning and convenient maintenance.

[0006] To solve the above technical problems, the technical solution adopted by the present application is:

[0007] The automatic pipetting device is characterized in that: a main air pump and a main one-way valve are arranged at the top of a pipette body, the main air pump is located above the main one-way valve, the main one-way valve can only guide air out of the pipette body and cannot guide air into the pipette body; an air branch pipe is arranged at the upper part of the pipette body, the air branch pipe is in communication with the pipette body, and the air branch pipe is located below the main one-way valve; an electric control valve is arranged at the end of the air branch pipe and is used for controlling the opening and closing of the air branch pipe and the outside world; an air extraction branch pipe is arranged at the upper part of the pipette body, the air extraction branch pipe is in communication with the pipette body, and the air extraction branch pipe is located below the main one-way valve; an auxiliary air pump is arranged at the end of the air extraction branch pipe; an auxiliary one-way valve is arranged near the connection position of the air extraction branch pipe and the pipette body; the auxiliary one-way valve can only guide air out of the pipette body and cannot guide air into the pipette body; a conductive anode I, a conductive cathode I, a conductive anode II and a conductive cathode II are arranged on the pipette body; the conductive anode I and the conductive cathode I form a group; the conductive anode II and the conductive cathode II form a group; the conductive anode I and the conductive cathode I are located at the maximum range of the pipette body; the volume corresponding to the position of the conductive anode II and the conductive cathode II is smaller than the maximum range.

[0008] One end of the conductive anode wire I is arranged on the conductive anode I, and the other end of the conductive anode wire I is arranged on the control module; one end of the conductive cathode wire I is arranged on the conductive cathode I, and the other end of the conductive cathode wire I is arranged on the control module.

[0009] One end of the conductive anode wire II is arranged on the conductive anode II, and the other end of the conductive anode wire II is arranged on the control module; one end of the conductive cathode wire II is arranged on the conductive cathode II, and the other end of the conductive cathode wire II is arranged on the control module.

[0010] One end of the main air pump wire is arranged on the main air pump, and the other end of the main air pump wire is arranged on the control module; one end of the auxiliary air pump wire is arranged on the auxiliary air pump, and the other end of the auxiliary air pump wire is arranged on the control module.

[0011] One end of the electric control valve wire is arranged on the electric control valve, and the other end of the electric control valve wire is arranged on the control module, which is used for power supply and switching of the electric control valve; the regulator is a regulating device for controlling the whole automatic pipetting device; one end of the regulator wire is arranged on the regulator, and the other end of the regulator wire is arranged on the control module.

[0012] The base is the basic platform of the automatic pipetting device, a plurality of bottom feet are arranged at the bottom of the base, the bottom feet can be rotatably adjusted in height, and the base is suitable for uneven table top; a support rod is arranged on the base, and a control module is arranged at the upper end of the support rod.

[0013] The movable support rod is arranged on the support rod and is fixed by an adjusting bolt, and the movable support rod can move up and down on the support rod to adjust the height of the pipette body.

[0014] The chuck is arranged at the tail end of the movable support rod and is used for fixing the pipette body.

[0015] The application also relates to a use method of the automatic pipetting device, and the use steps of the use method of the automatic pipetting device are as follows:

[0016] S1. A beaker containing a solution is arranged, the adjusting bolt is loosened, the movable support rod is adjusted to a proper height, the lower end of the pipette body is arranged below the liquid surface of the solution to be sucked, the main air pump is controlled to work by using the controller, air in the pipette body is discharged, and the solution is sucked;

[0017] S2. When the solution reaches the positions of the conductive anode II and the conductive cathode II, the loop formed by the conductive anode II and the conductive cathode II is conducted, the main air pump is controlled to stop working by the control module, the auxiliary air pump with smaller power and higher accuracy is started to work, and the solution sucking speed is slowed down;

[0018] S3. When the solution reaches the positions of the conductive anode I and the conductive cathode I, the loop formed by the conductive anode I and the conductive cathode I is conducted, the auxiliary air pump is controlled to stop working by the control module, and the pipette body sucks sufficient solution;

[0019] S4. The adjusting bolt is loosened, the movable support rod is adjusted to a proper height, the lower end of the pipette body is arranged above the beaker containing the solution, and the beaker containing the solution is removed;

[0020] S5. A container for storing a specified volume of solution is arranged directly below the pipette body, the adjusting bolt is loosened, and the movable support rod is adjusted to a proper height, so that the lower end of the pipette body is arranged at a proper height in the container for storing the specified volume of solution.

[0021] After the step S5, the following steps are sequentially performed:

[0022] S6. The electric control valve is opened by using the controller, the pipette body is connected with the outside, and the solution in the pipette body 1 is dropped into the container for storing the specified volume of solution;

[0023] S7. The adjusting bolt is loosened, the movable support rod is adjusted to a proper height, the lower end of the pipette body 1 is arranged above the beaker containing the solution, and the container for storing the specified volume of solution is removed.

[0024] S8. Through the regulator, the pipette body is cleaned by sucking distilled water slightly exceeding the height of the conductive anode I and the conductive cathode I.

[0025] The working principle and beneficial effects of the technical solution of the present application are as follows:

[0026] When the automatic pipetting device performs automatic pipetting, the beaker containing the solution is placed, the adjusting bolt is loosened, the moving support rod is adjusted to an appropriate height, the lower end of the pipette body is placed below the liquid surface of the solution to be sucked, the main air pump is controlled to work by the regulator, the air in the pipette body is discharged, and the solution is sucked; when the solution reaches the positions of the conductive anode II and the conductive cathode II, the loop formed by the conductive anode II and the conductive cathode II is conducted, the control module controls the main air pump to stop working, and the auxiliary air pump with smaller power and higher precision is started to work, and the speed of sucking the solution is slowed down; when the solution reaches the positions of the conductive anode I and the conductive cathode I, the loop formed by the conductive anode I and the conductive cathode I is conducted, the control module controls the auxiliary air pump to stop working, and the pipette body sucks sufficient solution; the adjusting bolt is loosened, the moving support rod is adjusted to an appropriate height, the lower end of the pipette body is higher than the beaker containing the solution, and the beaker containing the solution is removed; the container for storing the specified volume of solution is placed directly below the pipette body; the adjusting bolt is loosened, the moving support rod is adjusted to an appropriate height, and the lower end of the pipette body is located at an appropriate height in the container for storing the specified volume of solution. The electric control valve is opened by the regulator, the pipette body and the outside are connected, the solution in the pipette body is dropped into the container for storing the specified volume of solution, the adjusting bolt is loosened, the moving support rod is adjusted to an appropriate height, the lower end of the pipette body is higher than the beaker containing the solution, and the container for storing the specified volume of solution is removed; the pipette body is cleaned by sucking distilled water slightly exceeding the height of the conductive anode I and the conductive cathode I through the regulator. The automatic pipetting device can solve the problems of low precision, complex operation, and large difficulty in washing the ear ball in the prior art, avoid the defect of liquid outflow caused by excessive vibration in the manual operation process, and has the advantages of high precision, simple operation, reliable use, stable work, high efficiency, easy cleaning, and convenient maintenance. BRIEF DESCRIPTION OF DRAWINGS

[0027] The content expressed by each figure in the present specification and the marks in the figures are briefly described as follows:

[0028] Figure 1 It is a structural schematic diagram of the automatic pipetting device;

[0029] Figure 2 It is a working process schematic diagram of the automatic pipetting device;

[0030] 1. pipette body; 2. main air pump; 3. air branch pipe; 4. electric control valve; 5. air branch pipe; 6 auxiliary air pump; 7. main one-way valve; 8. auxiliary one-way valve; 9. conductive anode I; 10. conductive cathode I; 11. conductive anode II; 12. conductive cathode II; 13. conductive anode wire I; 14. conductive cathode wire I; 15. conductive anode wire II; 16. conductive cathode wire II; 17. main air pump wire; 18. auxiliary air pump wire; 19. electric control valve wire; 20. control module; 21. regulator wire; 22. regulator; 23. clamp; 24. moving support rod; 25. adjusting bolt; 26. support rod; 27. base; 28. foot; 29. beaker. DETAILED DESCRIPTION

[0031] The specific embodiments of the present application and the shapes, structures, mutual positions and connection relationships between the parts, functions and working principles of the components involved will be further described in detail below with reference to the drawings and the description of the embodiments:

[0032] As shown in the drawings Figure 1 , the drawings Figure 2As shown, the present application is an automatic pipetting device, the main air pump 2 and the main one-way valve 7 are arranged at the top of the pipette body 1, the main air pump 2 is located above the main one-way valve 7, the main one-way valve 7 can only lead air out of the pipette body 1, and cannot lead air into the pipette body 1; the air branch pipe 3 is arranged on the upper part of the pipette body 1, the air branch pipe 3 is communicated with the pipette body 1, and the air branch pipe 3 is located below the main one-way valve 7; the electric control valve 4 is arranged at the end of the air branch pipe 3, and is used for controlling the opening and closing of the air branch pipe 3 and the outside; the air suction branch pipe 5 is arranged on the upper part of the pipette body 1, the air suction branch pipe 5 is communicated with the pipette body 1, and the air suction branch pipe 5 is located below the main one-way valve 7; the auxiliary air pump 6 is arranged at the end of the air suction branch pipe 5; the auxiliary one-way valve 8 is arranged near the connection between the air suction branch pipe 5 and the pipette body 1; the auxiliary one-way valve 8 can only lead air out of the pipette body 1, and cannot lead air into the pipette body 1; the conductive anode I 9, the conductive cathode I 10, the conductive anode II 11 and the conductive cathode II 12 are arranged on the pipette body 1; the conductive anode I 9 and the conductive cathode I 10 form a group; the conductive anode II 11 and the conductive cathode II 12 form a group; the conductive anode I 9 and the conductive cathode I 10 are located at the maximum range of the pipette body 1; the corresponding position volume of the conductive anode II 11 and the conductive cathode II 12 is slightly smaller than the maximum range. When automatic pipetting is performed, the beaker containing the solution is placed, the adjusting bolt 25 is loosened, the moving support rod 24 is adjusted to an appropriate height, the lower end of the pipette body 1 is placed below the liquid surface of the solution to be sucked, the main air pump 2 is controlled to work by the controller 22, the air in the pipette body 1 is discharged, and the solution is sucked; when the solution reaches the position of the conductive anode II 11 and the conductive cathode II 12, the loop formed by the conductive anode II 11 and the conductive cathode II 12 is conducted, the main air pump 2 is controlled to stop working by the control module 20, the auxiliary air pump 6 with smaller power and higher accuracy is started to work, and the solution suction speed is slowed down; when the solution reaches the position of the conductive anode I 9 and the conductive cathode I 10, the loop formed by the conductive anode I 9 and the conductive cathode I 10 is conducted, the auxiliary air pump 6 is controlled to stop working by the control module 20, and the pipette body 1 sucks sufficient solution; the adjusting bolt 25 is loosened, the moving support rod 24 is adjusted to an appropriate height, the lower end of the pipette body 1 is higher than the beaker containing the solution, and the beaker containing the solution is removed; the container for storing the solution with a specified volume is placed directly below the pipette body 1; the adjusting bolt 25 is loosened, the moving support rod 24 is adjusted to an appropriate height, and the lower end of the pipette body 1 is located at an appropriate height in the container for storing the solution with a specified volume. The electric control valve 4 is opened by the controller 22, the pipette body 1 and the outside are connected, the solution in the pipette body 1 is dropped into the container for storing the solution with a specified volume, the adjusting bolt 25 is loosened, the moving support rod 24 is adjusted to an appropriate height, the lower end of the pipette body 1 is higher than the beaker containing the solution, and the container for storing the solution with a specified volume is removed; the distilled water slightly exceeding the height of the position of the conductive anode I 9 and the conductive cathode I 10 is sucked to clean the pipette body 1 by the controller 22.The automatic pipetting device can solve the problems of low precision, complex operation and large operation difficulty of the ear ball in the prior art, avoid the defect of liquid outflow caused by excessive vibration in the manual operation process, and has the advantages of high precision, simple operation, reliable use, stable work, high efficiency, easy cleaning and convenient maintenance.

[0033] The conductive anode wire 113 is arranged at one end of the conductive anode 109, and the other end of the conductive anode wire 113 is arranged on the control module 20.

[0034] The conductive anode wire 115 is arranged at one end of the conductive anode 111, and the other end of the conductive anode wire 115 is arranged on the control module 20.

[0035] The main air pump wire 17 is arranged at one end of the main air pump 2, and the other end of the main air pump wire 17 is arranged on the control module 20.

[0036] The electric control valve wire 19 is arranged at one end of the electric control valve 4, and the other end of the electric control valve wire 19 is arranged on the control module 20, which is used for power supply and switching of the electric control valve 4.

[0037] The base 27 is the basic platform of the automatic pipetting device, and a plurality of bottom feet 28 are arranged at the bottom of the base 27.

[0038] The moving support rod 24 is arranged on the support rod 26 and is fixed by the adjusting bolt 25.

[0039] The clamp 23 is arranged at the end of the moving support rod 24 and is used for fixing the pipette body 1.

[0040] The application also relates to a use method of the automatic pipetting device.

[0041] S1. Set the beaker containing the solution, loosen the adjusting bolt 25, adjust the mobile support rod 24 to the appropriate height, so that the lower end of the pipette body 1 is below the liquid level of the solution to be taken, use the controller 22 to control the main air pump 2 to work, so that the air in the pipette body 1 is discharged, and the solution is sucked; S2. When the solution reaches the position of the conductive anode II 11 and the conductive cathode II 12, the loop formed by the conductive anode II 11 and the conductive cathode II 12 is conducted, the control module 20 controls the main air pump 2 to stop working, and the auxiliary air pump 6 with smaller power and higher precision is started to work, and the speed of sucking the solution is slowed down; S3. When the solution reaches the position of the conductive anode I 9 and the conductive cathode I 10, the loop formed by the conductive anode I 9 and the conductive cathode I 10 is conducted, the control module 20 controls the auxiliary air pump 6 to stop working, and the pipette body 1 sucks the solution; S4. Loosen the adjusting bolt 25, adjust the mobile support rod 24 to the appropriate height, so that the lower end of the pipette body 1 is higher than the beaker containing the solution, and the beaker containing the solution is removed; S5. Place the container for storing the specified volume of solution directly below the pipette body 1; loosen the adjusting bolt 25, adjust the mobile support rod 24 to the appropriate height, so that the lower end of the pipette body is located at the appropriate height in the container for storing the specified volume of solution.

[0042] After the step S5, the following steps are further carried out: S6. Use the controller 22 to open the electric control valve 4, connect the pipette body 1 and the outside, and let the solution in the pipette body 1 drip into the container for storing the specified volume of solution; S7. Loosen the adjusting bolt 25, adjust the mobile support rod 24 to the appropriate height, so that the lower end of the pipette body 1 is higher than the beaker containing the solution, and the container for storing the specified volume of solution is removed; S8. Through the controller 22, distilled water slightly higher than the height of the conductive anode I 9 and the conductive cathode I 10 is sucked to clean the pipette body 1.

[0043] The automatic pipetting device provided by the application comprises a pipette body 1, a main air pump 2, a ventilation branch pipe 3, an electric control valve 4, an air pump branch pipe 5, an auxiliary air pump 6, a main one-way valve 7, an auxiliary one-way valve 8, a conductive anode I 9, a conductive cathode I 10, a conductive anode II 11, a conductive cathode II 12, a conductive anode wire I 13, a conductive cathode wire I 14, a conductive anode wire II 15, a conductive cathode wire II 16, a main air pump wire 17, an auxiliary air pump wire 18, an electric control valve wire 19, a control module 20, a controller wire 21, a controller 22, a chuck 23, a mobile support rod 24, an adjusting bolt 25, a support rod 26, a base 27, a foot 28 and a beaker 29.

[0044] The application has been described above by way of example with reference to the drawings. Obviously, the specific implementation of the application is not limited to the above-mentioned manner, and various improvements or direct applications of the technical concept and solution of the application to other occasions are within the protection scope of the application.

Claims

1. An automatic pipetting device, characterized in that: The main air pump (2) and the main check valve (7) are located at the top of the pipette body (1). The main air pump (2) is located on the main check valve (7). The main check valve (7) can only discharge air to the outside of the pipette body (1) and cannot introduce air into the pipette body (1). The venting branch pipe (3) is located at the top of the pipette body (1). The venting branch pipe (3) is connected to the pipette body (1). The venting branch pipe (3) is located below the main check valve (7). The electrically controlled valve (4) is located at the end of the venting branch pipe (3) and is used to control the opening and closing of the venting branch pipe (3) to the outside. The suction branch pipe (5) is located at the top of the pipette body (1). The suction branch pipe (5) is connected to the pipette body (1). The suction branch pipe (5) is located below the main check valve (7). The auxiliary air pump (6) The auxiliary one-way valve (8) is located at the end of the suction branch pipe (5); the auxiliary one-way valve (8) is located near the connection between the suction branch pipe (5) and the pipette body (1); the auxiliary one-way valve (8) can only discharge air to the outside of the pipette body (1) and cannot introduce air into the pipette body (1); the conductive anode I (9), conductive cathode I (10), conductive anode II (11), and conductive cathode II (12) are located on the pipette body (1); the conductive anode I (9) and conductive cathode I (10) are a group; the conductive anode II (11) and conductive cathode II (12) are a group; the positions of the conductive anode I (9) and conductive cathode I (10) are at the maximum capacity of the pipette body (1); the volume of the positions corresponding to the conductive anode II (11) and conductive cathode II (12) is less than the maximum capacity.

2. The automatic pipetting device according to claim 1, characterized in that: One end of the conductive anode wire I (13) is set on the conductive anode I (9), and the other end of the conductive anode wire I (13) is set on the control module (20); one end of the conductive cathode wire I (14) is set on the conductive cathode I (10), and the other end of the conductive cathode wire I (14) is set on the control module (20).

3. The automatic pipetting device according to claim 1 or 2, characterized in that: One end of the conductive anode wire II (15) is set on the conductive anode II (11), and the other end of the conductive anode wire II (15) is set on the control module (20); one end of the conductive cathode wire II (16) is set on the conductive cathode II (12), and the other end of the conductive cathode wire II (16) is set on the control module (20).

4. The automatic pipetting device according to claim 1 or 2, characterized in that: One end of the main air pump wire (17) is set on the main air pump (2), and the other end of the main air pump wire (17) is set on the control module (20); one end of the auxiliary air pump wire (18) is set on the auxiliary air pump (6), and the other end of the auxiliary air pump wire (18) is set on the control module (20).

5. The automatic pipetting device according to claim 1 or 2, characterized in that: One end of the electric valve wire (19) is set on the electric valve (4), and the other end of the electric valve wire (19) is set on the control module (20) for power supply and switching of the electric valve (4); the controller (22) is a control device for controlling the entire automatic pipetting device; one end of the controller wire (21) is set on the controller (22), and the other end of the controller wire (21) is set on the control module (20).

6. The automatic pipetting device according to claim 1 or 2, characterized in that: The base (27) is the basic platform of the automatic pipetting device. Multiple feet (28) are provided at the bottom of the base (27). The support rod (26) is set on the base (27), and the control module (20) is set on the upper end of the support rod (26).

7. The automatic pipetting device according to claim 6, characterized in that: The movable support rod (24) is set on the support rod (26) and fixed by the adjusting bolt (25). The movable support rod (24) can move up and down on the support rod (26) to adjust the height of the pipette body (1).

8. The automatic pipetting device according to claim 7, characterized in that: The clamp (23) is located at the end of the movable support rod (24) and is used to fix the pipette body (1).

9. The method of using the automated pipetting device according to any one of claims 1 to 8, characterized in that: The steps for using the aforementioned automatic pipetting device are as follows: S1. Place the beaker containing the solution, loosen the adjusting bolt (25), adjust the moving support rod (24) to a suitable height, so that the lower end of the pipette body (1) is below the liquid surface of the solution to be absorbed, and use the controller (22) to control the main air pump (2) to expel the air in the pipette body (1) and draw in the solution; S2. When the solution reaches the positions of conductive anode II (11) and conductive cathode II (12), the circuit formed by conductive anode II (11) and conductive cathode II (12) is connected, and the control module (20) controls the main air pump (2) to stop working and starts the auxiliary air pump (6) to work, and the speed of sucking in the solution slows down. S3. When the solution reaches the positions of conductive anode I (9) and conductive cathode I (10), the circuit formed by conductive anode I (9) and conductive cathode I (10) is connected, the control module (20) controls the auxiliary air pump (6) to stop working, and the pipette body (1) draws in a sufficient amount of solution. S4. Loosen the adjusting bolt (25), adjust the moving support rod (24) to a suitable height so that the lower end of the pipette body (1) is higher than the beaker containing the solution, and remove the beaker containing the solution; S5. Place the container that needs to hold the specified volume of solution directly below the pipette body (1); loosen the adjusting bolt (25) and adjust the moving support rod (24) to a suitable height so that the lower end of the pipette body (1) is at a suitable height inside the container that holds the specified volume of solution.

10. The method of using the automatic pipetting device according to claim 9, characterized in that: After step S5, proceed with the following steps in sequence: S6. Use the regulator (22) to open the electric control valve (4) to connect the pipette body (1) with the outside world, so that the solution in the pipette body (1) is dripped into the container holding the specified volume of solution; S7. Loosen the adjusting bolt (25), adjust the moving support rod (24) to a suitable height so that the lower end of the pipette body (1) is higher than the beaker containing the solution, and remove it from the container containing the specified volume of solution; S8. Using the regulator (22), draw in distilled water slightly above the height of the conductive anode I (9) and conductive cathode I (10) to clean the pipette body (1).

Citation Information

Patent Citations

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