Disposable flat plate culture medium automatic opening, filling, closing, shaking integrated machine

Abstract

The application discloses a one-time flat plate culture medium automatic cover opening, filling, cover closing and oscillation integrated machine, which comprises a supporting frame, a workbench connected to the upper portion of the supporting frame, a baffle connected to the upper end of the workbench, a material box arranged between the baffles, a partition plate connected to one side of the material box, and a push plate arranged below the partition plate. The push plate is driven to move by a cylinder, a guide block, a push dish guide block connecting plate, a synchronous belt wheel, a synchronous belt, a hook dish connecting block, a hook dish shaft, a hook dish block and a hook dish block mounting plate are driven to move by the motor, the bottom of the culture dish is hooked, the culture dish is reciprocated, the culture dish is conveniently transmitted to the next process, the culture dish is automatically opened and closed by driving the push plate to move, the culture dish is filled by a filling needle, highly automatic operation is realized, and bacteria infection can be effectively avoided.

Description

Technical Field

[0001] This invention relates to the field of plate culture equipment technology, specifically to an integrated machine for automatic opening, filling, closing, and shaking of disposable plate culture media. Background Technology

[0002] Plate culture medium is the most commonly used form of solid culture medium for obtaining pure cultures of microorganisms. It is a solid surface of culture medium formed in a sterile petri dish after the solid medium has cooled and solidified. It is often simply called a plate culture medium, also known as a flat culture medium or plate culture medium. Plate culture medium is often used for single spore isolation, measuring mycelial growth rate, observing colony morphology, antagonism experiments, and measuring the number of miscellaneous bacteria in the inoculation space.

[0003] CN207826623U discloses a filling device for a plate culture medium filling line, comprising a conveyor belt with support legs at its lower end, support columns fixedly connected to the front and rear sides of the upper end of the conveyor belt, support rods fixedly connected to the upper ends of the support columns, a connecting rod fixedly connected to the middle of the right end of the support rods, a first cylinder fixedly connected to the left side of the lower end of the connecting rods, and a transmission rod connected to a support slide rod at the middle of the right end of the first cylinder. The support slide rod includes a cylindrical contact, contact balls, and a spring plate. The lower end of the support slide rod is embedded with the ball balls, and the upper part of the support slide rod is inserted into a slide groove via a slider. The right end of the inner cavity of the slide groove is embedded with a contact plate. This filling device for a plate culture medium filling line, through the spring plate and pressure plate structure, makes the connection between the cylindrical contact and the contact plate more stable; through the sealing plate and electromagnet structure, the equipment driver is simplified.

[0004] However, in actual production, existing disposable plate culture media still occasionally pose a risk of contamination due to the opening of the cap and exposure to laminar flow. There is no machine that can integrate opening, filling, and closing the cap in one step to meet customer needs. On the other hand, during the actual filling process, the high viscosity and poor flowability of the culture medium liquid require increasing the actual filling volume to achieve uniformity, which increases costs and makes it impossible to reduce the amount to be filled, thus increasing production costs and greatly reducing practicality. Summary of the Invention

[0005] The purpose of this invention is to provide an integrated machine for automatic opening, filling, closing, and shaking of disposable plate culture media. This addresses the issues raised in the background section regarding the risk of contamination that still exists in the actual production process of existing disposable plate culture media due to the opening and exposure to laminar flow. There is no machine that can integrate opening, filling, and closing to meet customer needs. Furthermore, during the actual filling process, the high viscosity and poor flowability of the culture medium necessitate increasing the actual filling volume for uniform filling, thus increasing costs and preventing the reduction of the required filling volume, thereby significantly reducing practicality.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an integrated machine for automatic opening, filling, closing, and shaking of disposable plate culture media, comprising a support frame, a worktable connected above the support frame, a baffle connected to the upper end of the worktable, a material hopper disposed between the baffles, a partition connected to one side of the material hopper, a push plate disposed below the partition, a door frame connected above the support frame, a high-efficiency blower connected above the door frame, a touch screen disposed on one side of the door frame, an upper pressure plate connected to one side of the worktable, a partition strip disposed at the lower end of the upper pressure plate, a peristaltic pump body disposed at the rear end of the touch screen, a peristaltic pump head mounted at the front end of the peristaltic pump body, the peristaltic pump head being connected to a filling needle via a silicone tube, the filling needle being mounted on a needle holder, a waste trough disposed below the filling needle, a vibrating plate disposed at the front end of the worktable, a vibration mechanism disposed below the vibrating plate, and a pushing mechanism disposed inside the worktable.

[0007] Preferably, the inside of the material box is provided with a material storage trough, and six material storage troughs are arranged at equal intervals.

[0008] Preferably, seven partitions are provided at equal intervals, and the distance between each partition is the same as the width of the storage tank.

[0009] Preferably, six peristaltic pump heads and six filling needles are arranged at equal intervals, and the peristaltic pump heads and the filling needles are interconnected.

[0010] Preferably, the pushing mechanism includes a first guide roller, a second guide roller, a synchronous pulley, a synchronous belt, a cylinder, a motor, a guide block, a pusher guide block connecting plate, a hook connecting block, a hook shaft, a hook block, and a hook block mounting plate. The first guide roller, the guide block, and the pusher guide block connecting plate are connected by a cylinder. The second guide roller is connected by the synchronous pulley, the synchronous belt, the motor, the hook connecting block, the hook shaft, the hook block, and the hook block mounting plate.

[0011] Preferably, the vibration mechanism includes an oscillating motor, an eccentric shaft, a connecting block, a connecting plate, a rocking plate, a synchronous pulley, and a synchronous belt. The eccentric shaft is provided at the upper end of the oscillating motor and is connected to the transmission end of the oscillating motor. The connecting block is provided at the upper end of the eccentric shaft and is connected to the eccentric shaft via a rotating shaft. The connecting plate is provided above the connecting block, and the rocking plate is fixedly connected to the upper end of the connecting plate. The synchronous pulley is provided at the lower end of the eccentric shaft and is connected to the synchronous belt.

[0012] Preferably, there are two of each of the first guide roller, the second guide roller, the guide block, the hook-and-dish connecting block, and the hook-and-dish shaft; six hook-and-dish blocks are evenly spaced; the cylinder is connected to the push-and-dish guide block connecting plate; the motor is connected to the synchronous pulley; and the synchronous belt is connected to the hook-and-dish connecting block.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] 1. This automatic open, fill, close, and shaker for disposable plate culture media uses a cylinder to drive the guide block and pusher plate connecting plate to move horizontally, which in turn drives the pusher plate to move, achieving reciprocating motion. This pusher plate opens the top of the culture dish, and the motor drives the synchronous pulley and synchronous belt to rotate, which in turn drives the hook plate connecting block, hook plate shaft, hook plate block, and hook plate mounting plate to move horizontally, hooking the bottom of the culture dish and achieving reciprocating motion. This facilitates the transfer of the culture dish to the next process. The automatic opening and closing of the culture dish is achieved by driving the pusher plate to move. At the same time, the culture dish is filled through the filling needle, achieving a high degree of automation and effectively avoiding bacterial infection.

[0015] 2. This disposable plate culture medium automatic opening, filling, closing, and shaking integrated machine, through the setting of the vibration mechanism, moves the culture dish to the upper end of the vibration plate. The vibration motor drives the step wheel and step belt to control the eccentric shaft and work. The eccentric shaft drives the shaking plate to shake, vibrating and evenly mixing the liquid inside the culture dish. This vibration makes the liquid inside the culture dish more evenly poured, greatly improving its practicality. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of an all-in-one machine according to an embodiment of the present invention. Figure 1 ;

[0017] Figure 2 This is a schematic diagram of the structure of an all-in-one machine according to an embodiment of the present invention. Figure 2 ;

[0018] Figure 3 This is a side view of an all-in-one machine according to an embodiment of the present invention;

[0019] Figure 4 for Figure 2 A schematic diagram of the push mechanism in the all-in-one machine;

[0020] Figure 5 for Figure 2 A schematic diagram of the vibration mechanism in the all-in-one machine;

[0021] Figure 6 for Figure 2 A magnified view of part A in the all-in-one machine.

[0022] In the diagram: 1. Support frame; 2. Workbench; 3. Baffle; 4. Material bin; 5. Partition; 6. Push plate; 7. Door frame; 8. Upper pressure plate; 9. Spacer; 10. Vibrating plate; 11. Filling needle; 12. Needle holder; 13. Peristaltic pump body; 14. Peristaltic pump head; 15. Touch screen; 16. High-efficiency blower; 17. Waste hopper; 18. Vibration mechanism; 19. Pushing mechanism; 20. Storage tank; 1801. Vibrating motor; 1802. Eccentric shaft; 1803 1804. Connecting block; 1805. Connecting plate; 1806. Shaking plate; 1807. Stepping belt pulley; 1808. Stepping belt; 1909. First guide roller; 1900. Second guide roller; 1900. Synchronous belt pulley; 1901. Synchronous belt; 1902. Cylinder; 1903. Motor; 1904. Guide block; 1905. Pushing plate guide block connecting plate; 1906. Hooking plate connecting block; 1917. Hooking plate shaft; 1918. Hooking plate; 1919. Hooking plate mounting plate. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Please see Figure 1-5This invention provides a technical solution: an automatic opener, filler, closeer, and vibrator for disposable plate culture media, comprising a support frame 1, a workbench 2 connected above the support frame 1, baffles 3 connected to the upper end of the workbench 2, a material hopper 4 disposed between the baffles 3, a partition 5 connected to one side of the material hopper 4, a pusher plate 6 disposed below the partition 5, a door frame 7 connected above the support frame 1, a high-efficiency blower 16 connected above the door frame 7, a touch screen 15 disposed on one side of the door frame 7, an upper pressure plate 8 connected to one side of the workbench 2, a partition strip 9 disposed at the lower end of the upper pressure plate 8, a peristaltic pump body 13 disposed at the rear end of the touch screen 15, a peristaltic pump head 14 mounted at the front end of the peristaltic pump body 13, the peristaltic pump head 14 being connected to a filling needle 11 via a silicone tube, the filling needle 11 being mounted on a needle holder 12, a waste trough 17 disposed below the filling needle 11, and a vibrating plate 10 disposed at the front end of the workbench 2. A vibration mechanism 18 is installed below the worktable 2, and a pushing mechanism 19 is installed inside the worktable 2. First, the high-efficiency fan 16 is turned on, and the culture dish is placed into the storage tank 20 inside the material box 4. Next, the pusher plate 6 pushes the culture dish forward to the connection between the upper pressure plate 8 and the lower partition 9. At this time, the bottom of the culture dish is automatically separated, the pusher plate 6 returns to the starting position, and then the motor 1906 moves the bottom of the dish forward to the filling position through the hook block 1911. The system controls the peristaltic pump head 14 and the filling needle 11 to automatically fill. After filling is completed, the pusher plate 6 pushes the dish again to push out the next row of culture dishes until the bottom of this row of dishes is automatically closed to the bottom of the upper row of dishes. At this time, the motor 1906 moves the bottom of the dish forward to the filling position through the hook block 1911, and the system automatically fills. At this time, the culture dish that has completed the filling and closing action is pushed to the vibration mechanism 18, and the vibration motor 1801 is automatically turned on to shake the culture dish. This cycle is repeated.

[0025] Furthermore, the inside of the material box 4 is provided with a material storage trough 20, and six material storage troughs 20 are equally spaced; the material storage troughs 20 facilitate the better stacking of culture dishes.

[0026] Furthermore, seven partitions 5 are evenly spaced, and the distance between each partition 5 is the same as the width of the storage tank 20; the partitions 5 facilitate better operation of the petri dish.

[0027] Furthermore, six peristaltic pump heads 14 and six filling needles 11 are equally spaced, and the peristaltic pump heads 14 and the filling needles 11 are interconnected; the generated filling waste can be directly discharged into the waste tank 17, and automatic filling can be achieved through PLC program control.

[0028] Furthermore, the driving mechanism 19 includes a first guide roller 1901, a second guide roller 1902, a synchronous pulley 1903, a synchronous belt 1904, a cylinder 1905, a motor 1906, a guide block 1907, a pusher guide block connecting plate 1908, a hook connecting block 1909, a hook shaft 1910, a hook block 1911, and a hook block mounting plate 1912. The first guide roller 1901 passes through the guide block 1907. The pusher guide block connecting plate 1908 is connected to the cylinder 1905 and the guide block 1907. The second guide roller 1902 is connected to the synchronous belt 1904, the motor 1906, and the hook connecting block via the synchronous pulley 1903. 1909, hooking shaft 1910, hooking block 1911, and hooking block mounting plate 1912 are connected; the operation of cylinder 1905 drives guide block 1907 and pusher guide block connecting plate 1908 to move horizontally, thereby driving push plate 6 to move, realizing the reciprocating motion of push plate 6, which pushes open the top cover of the culture dish. The motor 1906 drives synchronous pulley 1903 and synchronous belt 1904 to rotate, thereby driving hooking block 1909, hooking shaft 1910, hooking block 1911, and hooking block mounting plate 1912 to move horizontally, hooking the bottom of the culture dish, realizing the reciprocating motion of the culture dish, and facilitating the transfer of the culture dish to the next process.

[0029] Furthermore, the vibration mechanism 18 includes an oscillating motor 1801, an eccentric shaft 1802, a connecting block 1803, a connecting plate 1804, a rocking plate 1805, a stepping belt pulley 1806, and a stepping belt 1807. The eccentric shaft 1802 is located at the upper end of the oscillating motor 1801 and is connected to the transmission end of the oscillating motor 1801. The connecting block 1803 is located at the upper end of the eccentric shaft 1802 and is connected to the eccentric shaft 1802 via a rotating shaft. A connecting plate 1804 is provided above 1803, and a shaking plate 1805 is fixedly connected to the upper end of the connecting plate 1804. A stepping wheel 1806 is provided at the lower end of the eccentric shaft 1802, and the stepping wheel 1806 is connected to the stepping belt 1807. The eccentric shaft 1802 and the connecting block 1803 are controlled by the stepping wheel 1806 and the stepping belt 1807 driven by the oscillating motor 1801. The eccentric shaft 1802 drives the shaking plate 1805 to shake, thereby vibrating and mixing the liquid inside the petri dish.

[0030] Furthermore, there are two of each of the following: first guide roller 1901, second guide roller 1902, guide block 1907, hook-and-dish connecting block 1909, and hook-and-dish shaft 1910. There are six hook-and-dish blocks 1911 arranged at equal intervals. Cylinder 1905 is connected to push-and-dish guide block connecting plate 1908. Motor 1906 is connected to synchronous pulley 1903. Synchronous belt 1904 is connected to hook-and-dish connecting block 1909.

[0031] Furthermore, the first guide roller 1901 and the second guide roller 1902 are both connected inside the worktable 2; the first guide roller 1901 and the second guide roller 1902 limit the pushing mechanism 19, thereby enhancing the stability of the structure.

[0032] Working principle: First, turn on the high-efficiency fan 16 and place the culture dish into the storage tank 20 inside the material box 4. Next, the pusher plate 6 pushes the culture dish forward into the connection between the upper pressure plate 8 and the lower partition strip 9. At this time, the bottom of the culture dish automatically separates, and the pusher plate 6 returns to the starting position. Then, the motor 1906 moves the bottom of the dish forward to the filling position through the hook block 1911. The system controls the peristaltic pump head 14 and the filling needle 11 to automatically fill. After filling is completed, the pusher plate 6 pushes the dish again to push out the next row of culture dishes until the lid of this row of dishes automatically closes to the bottom of the upper row of dishes. At this time, the motor 1906 moves the bottom of the dish forward to the filling position through the hook block 1911, and the system automatically fills. At this time, the culture dishes that have completed the filling and closing action are pushed to the vibration mechanism 18. The vibration motor 1801 automatically turns on to shake the culture dishes, and this cycle repeats.

[0033] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. An automatic plate culture medium opening, filling, closing, and shaking integrated machine, including a support frame (1), characterized in that: A workbench (2) is connected above the support frame (1). A baffle (3) is connected to the upper end of the workbench (2). A material box (4) is arranged between the baffles (3). A partition (5) is connected to one side of the material box (4). A push plate (6) is arranged below the partition (5). A door frame (7) is connected above the support frame (1). A high-efficiency fan (16) is connected above the door frame (7). A touch screen (15) is arranged on one side of the door frame (7). An upper pressure plate (8) is connected to one side of the workbench (2). A lower end of the upper pressure plate (8) is provided with... The partition (9), the touch screen (15) is provided with a peristaltic pump body (13) at the rear end, the peristaltic pump body (13) is provided with a peristaltic pump head (14) at the front end, the peristaltic pump head (14) is connected to the filling needle (11) through a silicone tube, the filling needle (11) is installed on the needle holder (12), the filling needle (11) is provided with a waste trough (17) below the filling needle (11), the workbench (2) is provided with a vibration plate (10) at the front end, the vibration plate (10) is provided with a vibration mechanism (18) below the vibration plate (10), and the workbench (2) is provided with a pushing mechanism (19) inside. The pushing mechanism (19) includes a first guide roller (1901), a second guide roller (1902), a synchronous pulley (1903), a synchronous belt (1904), a cylinder (1905), a motor (1906), a guide block (1907), a pusher guide block connecting plate (1908), a hooker connecting block (1909), a hooker shaft (1910), a hooker block (1911), and a hooker block mounting plate (1912). The first guide roller (1901) passes through the guide block (1907). The pusher guide block connecting plate (1908) is connected to the cylinder (1905) and the guide block (1907). The second guide roller (1902) is connected to the synchronous belt (1904), the motor (1906), the hooker connecting block (1909), the hooker shaft (1910), the hooker block (1911), and the hooker block mounting plate (1912) through the synchronous pulley (1903). The vibration mechanism (18) includes an oscillating motor (1801), an eccentric shaft (1802), a connecting block (1803), a connecting plate (1804), a rocking plate (1805), a stepping wheel (1806), and a stepping belt (1807). The upper end of the oscillating motor (1801) is provided with an eccentric shaft (1802), which is connected to the transmission end of the oscillating motor (1801). A connecting block (1803) is provided at the upper end of the eccentric shaft (1802) via a rotating shaft. A connecting plate (1804) is provided above the connecting block (1803). A rocking plate (1805) is fixedly connected to the upper end of the connecting plate (1804). A stepping wheel (1806) is provided at the lower end of the eccentric shaft (1802). The stepping wheel (1806) is connected to the stepping belt (1807).

2. The automatic opener, filler, capper, and shaker for disposable plate culture media according to claim 1, characterized in that: The material box (4) has a storage trough (20) inside, and six storage troughs (20) are evenly spaced.

3. The automatic disposable plate culture medium opening, filling, closing, and shaking integrated machine according to claim 1, characterized in that: The partitions (5) are arranged at equal intervals, and the distance between each partition (5) is the same as the width of the storage tank (20).

4. The automatic disposable plate culture medium opening, filling, closing, and shaking integrated machine according to claim 1, characterized in that: The peristaltic pump head (14) and filling needle (11) are arranged in six equal intervals, and the peristaltic pump head (14) and filling needle (11) are connected to each other.

5. The automatic disposable plate culture medium opening, filling, closing, and shaking integrated machine according to claim 1, characterized in that: Two of each of the first guide roller (1901), the second guide roller (1902), the guide block (1907), the hook-and-dish connecting block (1909), and the hook-and-dish shaft (1910) are provided. Six hook-and-dish blocks (1911) are provided at equal intervals. The cylinder (1905) is connected to the push-and-dish guide block connecting plate (1908). The motor (1906) is connected to the synchronous pulley (1903). The synchronous belt (1904) is connected to the hook-and-dish connecting block (1909).

6. The automatic disposable plate culture medium opening, filling, closing, and shaking integrated machine according to claim 1, characterized in that: The first guide roller (1901) and the second guide roller (1902) are both connected inside the worktable (2).

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