Curcuma phaeocaulis rootstock extract emulsion, and preparation method and application thereof

Abstract

The present application relates to a kind of turmeric rhizome extract emulsion and its preparation method and application, the component of the turmeric rhizome extract emulsion includes 30-60% turmeric rhizome extract, 5-15% emulsifier, 1-10% alcohol solvent, 30-60% water by mass percentage, the component in the specific mass ratio of turmeric rhizome extract, emulsifier, alcohol solvent and water is compounded and prepared a new emulsion for preventing and treating plant powdery mildew with turmeric rhizome extract as active ingredient, it has the advantages of simple preparation process, convenient to use, good stability, powdery mildew prevention and treatment effect good.Compared with chemical synthesis pesticide, turmeric rhizome extract is derived from natural plant, low toxicity, and it is not easy to produce drug resistance.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticide preparation, and relates to a curcuma rhizome extract emulsion in water, a preparation method and application thereof, in particular to a curcuma rhizome extract emulsion in water, a preparation method and application thereof in the prevention and treatment of plant powdery mildew. BACKGROUND

[0002] As a serious disease of crops, once crops are infected with powdery mildew, the disease develops rapidly, recurs frequently, is difficult to eradicate, and even affects the next crop, causing serious consequences. At present, the chemical pesticide prevention and treatment of powdery mildew has problems such as drug resistance, safety, low fungicidal activity and large amount of drug use, which seriously restricts the production of crops and causes the reduction of crops. An effective biological preparation prevention and treatment scheme is urgently needed.

[0003] At present, the green and safe biological pesticide for preventing and treating fruit tree diseases mainly includes living microorganism pesticides, part of plant source pesticides and part of agricultural antibiotics. Compared with chemical pesticides, most of the biological pesticides have slow effect and poor prevention and treatment effect, and especially the living microorganism pesticides which are safe to the environment are mainly used for preventing the occurrence of diseases, and have relatively poor prevention and treatment effect after the occurrence of diseases. Therefore, a rapid, safe and efficient prevention and treatment method for the occurrence of plant powdery mildew is urgently needed.

[0004] Plant source pesticides are new pesticides developed by using plants themselves or effective active ingredients extracted therefrom to prevent and treat plant diseases, insect pests and weed pests. Among numerous plant resources in the world, many plant resources contain effective active substances for preventing and treating diseases and insect pests, including pyrethrum, neem tree and the like. Plant resources for developing plant source pesticides are very rich, and plant source pesticides have broad industrialization prospects.

[0005] In the process of researching and developing plant source pesticides, the plant effective substance extract obtained cannot be directly used, and some adjuvants need to be added to make it into a stable, safe and efficient pesticide preparation before use. At present, the dosage forms of pesticides mainly include the following types: suspension concentrate (SC), emulsifiable concentrate (EC), microemulsion (ME), emulsion in water (EW), wettable powder (WP), water dispersible granule (WDG, also known as dry suspension concentrate), aqueous agent (AS), granule (GR) and microcapsule suspension concentrate (CS). SUMMARY

[0006] In view of the deficiencies of the prior art, the present application aims to provide a curcuma rhizome extract emulsion in water, a preparation method and application thereof, in particular to provide a curcuma rhizome extract emulsion in water, a preparation method and application thereof in the prevention and treatment of plant powdery mildew.

[0007] To achieve the object of the present application, the following technical solutions are adopted:

[0008] In a first aspect, the present application provides a curcuma rhizome extract emulsion in water, the components of the curcuma rhizome extract emulsion in water include 30-60% curcuma rhizome extract, 5-15% emulsifier, 1-10% alcohol solvent, 30-60% water in terms of mass percentage.

[0009] The present application prepares a new emulsion in water for preventing and treating plant powdery mildew by compounding the components of curcuma rhizome extract, emulsifier, alcohol solvent and water in a certain mass ratio, which has the advantages of simple preparation process, convenient use, good stability, good effect of preventing and treating powdery mildew. Compared with chemical synthetic pesticides, curcuma rhizome extract is derived from natural plants and will not accumulate in the environment to destroy the environment, but will degrade in the environment relatively quickly; low toxicity; at the same time, compared with chemical synthetic pesticides with single component, the curcuma rhizome extract prepared by the present application has complex components, and the prevention and treatment of plant powdery mildew is the result of the mutual cooperation of multiple effective components, and other non-effective components also play an auxiliary role, so the target is not easy to produce drug resistance.

[0010] The mass percentage of the curcuma rhizome extract can be selected from 30%, 35%, 38%, 40%, 45%, 50%, 52%, 55%, 60% and the like.

[0011] The mass percentage of the emulsifier can be selected from 5%, 8%, 9%, 10%, 12%, 15% and the like.

[0012] The mass percentage of the alcohol solvent can be selected from 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10% and the like.

[0013] The mass percentage of the water can be selected from 30%, 35%, 38%, 40%, 45%, 50%, 52%, 55%, 60% and the like.

[0014] Other specific point values within the above numerical ranges can be selected, which will not be described here.

[0015] When the four components of curcuma rhizome extract, emulsifier, alcohol solvent and water are combined in the above more preferred mass ratio, the product has more excellent stability, and the curcuma rhizome extract can more stably and durably exert its effect of preventing and treating powdery mildew.

[0016] Preferably, the emulsifier includes a non-ionic emulsifier and / or an anionic emulsifier, preferably a combination of a non-ionic emulsifier and an anionic emulsifier.

[0017] Preferably, the non-ionic emulsifier includes a polyoxyethylene ether non-ionic emulsifier or a polyoxyethylene sulfonyl castor oil non-ionic emulsifier.

[0018] Preferably, the anionic emulsifier comprises a sulfonate anionic emulsifier or a benzene sulfonate anionic emulsifier.

[0019] The present application optimizes the formulation of the emulsifier, which is better matched with other components, thereby making the system more stable and ensuring the long-term and stable exertion of the curcuma rhizome extract. The following emulsifier formulation is a more preferred formulation.

[0020] Preferably, the non-ionic emulsifier comprises YUS-FS1 or YUS-CP120.

[0021] Preferably, the anionic emulsifier comprises YUS-A51G or YUS-A41B.

[0022] Preferably, the alcohol solvent comprises any one or a combination of at least two of ethylene glycol, polyethylene glycol, propylene glycol, glycerol or glycerin.

[0023] Preferably, the alcohol solvent is a combination of ethylene glycol and glycerol.

[0024] The present application also optimizes the formulation of the alcohol solvent, which further ensures the solubility and stability of the curcuma rhizome extract in the system.

[0025] Preferably, the curcuma rhizome extract is prepared by a preparation method comprising the following steps:

[0026] (1) drying and crushing curcuma rhizome raw materials, then extracting with an ethanol aqueous solution to obtain an extract;

[0027] (2) concentrating the extract and extracting with an organic solvent to obtain an aqueous phase product and an oil phase product;

[0028] (3) subjecting the oil phase product to molecular distillation to remove curcuma oleoresin to obtain the curcuma rhizome extract.

[0029] The curcuma rhizome extract prepared by the above specific preparation process is more operable as a raw material for water emulsion, and is more suitable for industrial scale-up production. The curcuma rhizome extract prepared by the above specific preparation process has stable properties and a long storage period. At the same time, the curcuma rhizome extract is very friendly to the environment, has low toxicity to land environment species such as birds, bees and silkworms in the application environment, and has more excellent effects of preventing and treating plant powdery mildew, especially preventing and treating powdery mildew of legume plants, rosaceous plants and grape plants.

[0030] Preferably, the concentration of the ethanol aqueous solution is 65-85%, for example, 65%, 68%, 70%, 72%, 75%, 78%, 80%, 82%, 85% and the like. Other specific point values within the numerical range can be selected, which will not be described here one by one.

[0031] The concentration of the aqueous ethanol solution is more preferably 65-85% because the curcuma rhizome extract prepared using the aqueous ethanol solution in this concentration range has more significant efficacy in preventing and treating plant powdery mildew compared to products of other concentrations.

[0032] Preferably, the solid-liquid ratio of the curcuma rhizome raw material to the aqueous ethanol solution is (30-70) g / L, for example 30 g / L, 35 g / L, 40 g / L, 45 g / L, 50 g / L, 55 g / L, 60 g / L, 65 g / L, 70 g / L, etc., and other specific point values in the above numerical range can be selected, which will not be repeated here.

[0033] Preferably, the extraction temperature is 15-30℃, for example 15℃, 20℃, 25℃, 30℃, etc., and the extraction time is 30-50h, for example 30h, 35h, 40h, 45h, 48h, 50h, etc., and other specific point values in the above numerical range can be selected, which will not be repeated here.

[0034] Preferably, the pressure condition of the molecular distillation is 0.1-5 Pa, for example 0.1 Pa, 0.5 Pa, 1 Pa, 1.5 Pa, 2 Pa, 2.5 Pa, 3 Pa, 3.5 Pa, 4 Pa, 5 Pa, etc., and the temperature of the molecular distillation is 110-130℃, for example 110℃, 115℃, 120℃, 125℃, 130℃, etc., and other specific point values in the above numerical range can be selected, which will not be repeated here.

[0035] The curcuma rhizome extract prepared under the above more preferred molecular distillation conditions has more significant efficacy in preventing and treating plant powdery mildew, especially efficacy in preventing and treating powdery mildew of leguminous plants, rosaceous plants, and vitaceous plants.

[0036] In a second aspect, the present application provides a preparation method of the curcuma rhizome extract emulsion according to the first aspect, which comprises:

[0037] The formula amount of the emulsifier and water are mixed for the first emulsification, and then mixed with the curcuma rhizome extract and the alcohol solvent for the second emulsification, and the curcuma rhizome extract emulsion is obtained.

[0038] Preferably, the emulsification is carried out under stirring, and the stirring speed is 3000-6000 rpm, for example 3000 rpm, 3500 rpm, 4000 rpm, 4500 rpm, 5000 rpm, 5500 rpm, 6000 rpm, etc., and other specific point values in the above numerical range can be selected, which will not be repeated here.

[0039] Preferably, the time for the first emulsification and the second emulsification is independently 5-15 minutes, for example, 5 minutes, 6 minutes, 7 minutes, 8 minutes, 10 minutes, 12 minutes, 14 minutes, 15 minutes, etc. Other specific values ​​within this numerical range can be selected and will not be described here one by one.

[0040] In a third aspect, the present invention provides use of the turmeric rhizome extract aqueous emulsion according to the first aspect in preventing and controlling powdery mildew of plants.

[0041] Preferably, the plant comprises a plant of the family Leguminosae, a plant of the family Rosaceae or a plant of the family Vitaceae.

[0042] In a fourth aspect, the present invention provides a method for controlling powdery mildew in leguminous plants, rosaceous plants, or grape plants, comprising: applying an effective dose of the turmeric rhizome extract aqueous emulsion according to the first aspect to the leguminous plants, rosaceous plants, or grape plants.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] The present invention prepares a novel aqueous emulsion for preventing and controlling powdery mildew of plants, comprising a turmeric rhizome extract, an emulsifier, an alcohol solvent, and water in specific mass ratios. The aqueous emulsion comprises a turmeric rhizome extract as the active ingredient. The aqueous emulsion has a simple preparation process, is easy to use, and has the advantages of good stability and good powdery mildew prevention and control efficacy. Compared to chemically synthesized pesticides, turmeric rhizome extract is derived from natural plants and will not accumulate and damage the environment, but will degrade relatively quickly in the environment; it is also low in toxicity. Furthermore, compared to chemically synthesized pesticides with a single ingredient, the turmeric rhizome extract prepared by the present invention has a complex composition. The effect of preventing and controlling powdery mildew of plants is the result of the combined effect of multiple active ingredients, and some other non-active ingredients also play a supporting role, so the target is less likely to develop drug resistance. DETAILED DESCRIPTION

[0045] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0046] The emulsifier YUS-FS1 involved in the following examples is a product purchased from Yujieshi Chemical (Shanghai) Co., Ltd.; the emulsifier YUS-CP120 is a product purchased from Yujieshi Chemical (Shanghai) Co., Ltd.; the emulsifier YUS-A51G is a product purchased from Yujieshi Chemical (Shanghai) Co., Ltd.; and the emulsifier YUS-A41B is a product purchased from Yujieshi Chemical (Shanghai) Co., Ltd.

[0047] Preparation Example 1

[0048] The preparation example provides a curcuma rhizome extract, and a preparation method thereof is as follows:

[0049] (1) After the dried curcuma rhizome raw material is crushed, 70% ethanol aqueous solution is used for extraction (the material-liquid ratio is 60 g / L), the extraction temperature is 25°C, and the extraction time is 48 h, so that an extraction liquid is obtained;

[0050] (2) After the extraction liquid is concentrated, n-hexane is used for extraction, so that a water phase product and an oil phase product are obtained;

[0051] (3) The oil phase product is subjected to molecular distillation under the pressure condition of 4 Pa and at the temperature of 115°C, so that curcuma oleoresin is removed, and the curcuma rhizome extract is obtained.

[0052] Preparation example 2

[0053] The preparation example provides a curcuma rhizome extract, and a preparation method thereof is different from that of the preparation example 1 only in that the 70% ethanol aqueous solution is replaced by an equal amount of 55% ethanol aqueous solution, and other conditions remain unchanged.

[0054] Preparation example 3

[0055] The preparation example provides a curcuma rhizome extract, and a preparation method thereof is different from that of the preparation example 1 only in that the 70% ethanol aqueous solution is replaced by an equal amount of 95% ethanol aqueous solution, and other conditions remain unchanged.

[0056] Preparation example 4

[0057] The preparation example provides a curcuma rhizome extract, and a preparation method thereof is different from that of the preparation example 1 only in that the pressure condition of molecular distillation is 1 Pa, and the temperature is 140°C, and other conditions remain unchanged.

[0058] Preparation example 5

[0059] The preparation example provides a curcuma rhizome extract, and a preparation method thereof is different from that of the preparation example 1 only in that the pressure condition of molecular distillation is 8 Pa, and the temperature is 100°C, and other conditions remain unchanged.

[0060] Example 1

[0061] The example provides a curcuma rhizome extract emulsion, and a formula composition thereof is as follows: the curcuma rhizome extract prepared in the preparation example 1 is 50%, YUS-FS1 is 6.5%, YUS-A51G is 2%, ethylene glycol is 5%, and water is 36.5%.

[0062] A preparation method is as follows: the formula amount of YUS-FS1, YUS-A51G and water are mixed to be firstly emulsified at 4000 rpm for 10 min, then the curcuma rhizome extract and the alcohol solvent are mixed to be secondly emulsified at 5000 rpm for 10 min, and the curcuma rhizome extract emulsion is obtained.

[0063] Example 2

[0064] This embodiment provides a turmeric rhizome extract aqueous emulsion, the formulation of which is as follows: 50% of the turmeric rhizome extract prepared in Preparation Example 1, 4.5% of YUS-CP120, 1% of YUS-A51G, 7% of propylene glycol, and 37.5% of water.

[0065] The preparation method is as follows: YUS-CP120, YUS-A51G and water in a formulated amount are mixed and emulsified at 5000 rpm for 5 minutes for the first time, and then mixed with turmeric rhizome extract and alcohol solvent and emulsified again at 6000 rpm for 5 minutes to obtain the product.

[0066] Example 3

[0067] This embodiment provides a turmeric rhizome extract aqueous emulsion, the formulation of which is as follows: 50% of the turmeric rhizome extract prepared in Preparation Example 1, 8.5% of YUS-FS1, 3% of YUS-A41B, 3% of ethylene glycol, and 35.5% of water.

[0068] The preparation method is as follows: YUS-FS1, YUS-A41B and water in a formulated amount are mixed and emulsified at 3000 rpm for 15 minutes for the first time, and then mixed with turmeric rhizome extract and alcohol solvent and emulsified again at 5000 rpm for 10 minutes to obtain the product.

[0069] Examples 4-7

[0070] This example provides four turmeric rhizome extract aqueous emulsions, the formula composition of which differs from that of Example 1 only in that the turmeric rhizome extract prepared in Preparation Example 1 is replaced with the turmeric rhizome extracts prepared in Preparation Examples 2-5 in equal amounts, and the other components and contents remain unchanged.

[0071] Example 8

[0072] This example provides an aqueous emulsion of turmeric rhizome extract, the formula composition of which differs from that of Example 1 only in that YUS-FS1 6.5% and YUS-A51G 2% are replaced with YUS-FS1 8.5%, while the other components and contents remain unchanged.

[0073] Example 9

[0074] This embodiment provides an aqueous emulsion of turmeric rhizome extract, the formula composition of which differs from that of Example 1 only in that YUS-FS1 6.5% and YUS-A51G 2% are replaced with YUS-A51G 8.5%, while other components and contents remain unchanged.

[0075] Example 10

[0076] The present example provides a curcuma rhizome extract emulsion in water, the difference of the formula composition of which from that of Example 1 is only that the 5% ethylene glycol is replaced by 5% glycerol, and other components and contents remain unchanged.

[0077] Example 11

[0078] The present example provides a curcuma rhizome extract emulsion in water, the difference of the formula composition of which from that of Example 1 is only that the 5% ethylene glycol is replaced by a combination of 3% ethylene glycol and 2% glycerol, and other components and contents remain unchanged.

[0079] Test Example 1

[0080] The curcuma rhizome extract emulsion in water prepared in Examples 1-3 is characterized in the following properties:

[0081] (1) Emulsion stability evaluation: The sample is diluted 200 times with standard hard water, and tested according to GB / T 1603-2001. If there is no floating oil on the top and no sediment and precipitate on the bottom of the measuring cylinder, the emulsion stability is determined to be qualified.

[0082] (2) Low-temperature stability evaluation: The product sometimes needs to be stored and transported under relatively cold conditions, which requires the product to maintain good appearance and not to separate at a lower temperature. According to the method 2.1 in GB / T 19137-2003, the product is stored at 2℃ for 7 days, and the volume of the separated product is ≤0.3 mL to be qualified.

[0083] (3) Hot storage stability evaluation: Hot storage stability is an index for testing the storage period of pesticides. Hot storage stability test is to rapidly age the product by heating to indicate whether the product has good room temperature storage stability. According to the method 2.1 in GB / T 19136-2003, the product is stored at 55℃ for 14 days, and according to the evaluation requirements of the pesticide testing institute and the measured data of the product storage test, the mass fraction of ar-turmerone and the mass concentration of ar-turmerone after hot storage should not be less than 95% of that measured before hot storage, and the emulsion stability still meets the standard requirements.

[0084] The results are shown in Table 1.

[0085] Table 1

[0086]

[0087]

[0088] As can be seen from the data results in Table 1, the curcuma rhizome extract emulsion in water involved in the present application has excellent emulsion stability, hot storage stability and low-temperature stability, which can make the curcuma rhizome extract more stably and durably exert its efficacy of preventing and treating powdery mildew.

[0089] Test Example 2

[0090] A water emulsion of the turmeric rhizome extract prepared in Examples 1-11 was subjected to a test for the prevention of pea powdery mildew:

[0091] (1) Cultivation of pea seedlings: Pea seeds (variety "Sweet Pea") were soaked in water at room temperature for 24 hours in a petri dish, and then the water was poured out. The bottom of the petri dish was padded with moistened absorbent cotton, and the pea seeds were evenly dispersed in the petri dish. Water was poured every day to keep the absorbent cotton moist. After about a week, when the pea seedlings had grown to a height of about 2-3 cm, they were transplanted into small pots. Water was poured every two days, and when the first true leaves of the pea seedlings had grown and the second true leaves had emerged, healthy seedlings with the same growth were selected for the test.

[0092] (2) Preparation of a spore suspension of the pea powdery mildew pathogen (Erysphe pist DC.): The pea seedlings were inoculated with a conidial spore suspension of the pathogen by spraying, so a spore suspension of the appropriate concentration was prepared as follows:

[0093] (2.1) Pure water was poured into a small spray bottle, and a fresh powdery mildew spot on a leaf of a diseased plant was selected. The spores were washed off the leaf with the spray bottle and into a clean beaker.

[0094] (2.2) The spore suspension obtained was mixed well, and a drop was placed on a glass slide. The spores were observed under a microscope for fullness of form and for the number of spores in one field of view at a magnification of 15*10. The spore suspension was required to have full spores to ensure viability, and the number of spores in one field of view at a magnification of 15*10 was required to be about 20-30, not too few or too many. In order to ensure the viability of the spores, the spore suspension was required to be used within 2 hours of preparation.

[0095] (3) Inoculation of the pea powdery mildew pathogen:

[0096] (3.1) The prepared spore suspension was evenly sprayed on the pea leaves.

[0097] (3.2) The pea seedlings inoculated with the fungus were moved into a box, and the remaining spore suspension was sprayed in the box to create a high-humidity environment inside the box. The lid was then closed, and the seedlings were incubated at a temperature of about 25°C for 24 hours.

[0098] (3.3) After 24 hours, the lid was opened and the pea seedlings were removed. After the water on the leaves had dried, the seedlings were treated with the test agent.

[0099] (4) Method of application:

[0100] (4.1) Each group of water emulsions was diluted 200-fold with deionized water to give 0.1 ml, which was poured into a small spray bottle and labeled for use.

[0101] (4.2) Adjuvant control group of drug preparation: according to the preparation formula, the content of turmeric extract is replaced by water to make the adjuvant solution with the same concentration as the adjuvant contained in the preparation, 0.1 ml is diluted 200 times with water and then loaded into a small spray bottle, and labeled for standby;

[0102] (4.3) Positive control group of drug preparation: fungicide 430 g / L tebuconazole suspension is diluted 2000 times with water and then loaded into a small spray bottle, and labeled for standby;

[0103] (4.4) Blank control group preparation: load deionized water into a small spray bottle, and label for standby;

[0104] (4.5) Spray: divide the pea seedlings inoculated for 24 hours into 4 repeats according to each drug treatment, and evenly spray the corresponding drug on the leaf surface, taking the drug solution covering the leaf surface as the standard;

[0105] (4.6) Culture: after spraying, the pea seedlings are placed in an environment with a temperature of about 25℃ and a humidity of about 70%, and watered every two days;

[0106] (4.7) Observation: observe the phytotoxicity after 24 hours and 5 days after spraying, discard the preparations with serious phytotoxicity according to the phytotoxicity, start to count the incidence of the remaining preparation formulas after six days, calculate the control effect, and continuously observe and record the change of the control effect for several days.

[0107] (5) Efficacy evaluation:

[0108] Each group of samples is evaluated for 10 strains, and all the leaves of each strain are investigated, and the grading standards are as follows:

[0109] 0 level: no lesion;

[0110] 1 level: lesion area accounts for less than 5% of the entire leaf area;

[0111] 3 level: lesion area accounts for less than 6-15% of the entire leaf area;

[0112] 5 level: lesion area accounts for less than 16-25% of the entire leaf area;

[0113] 7 level: lesion area accounts for less than 26-50% of the entire leaf area;

[0114] 9 level: lesion area accounts for more than 51% of the entire leaf area.

[0115]

[0116]

[0117] The control effect data is shown in Table 2:

[0118] Table 2

[0119]

[0120]

[0121] From the data in Table 2, it can be seen that the turmeric root extract emulsion involved in the application has excellent effect on preventing and treating pea powdery mildew, and the preparation process conditions of the turmeric root extract and the formula of the emulsion also affect the prevention and treatment effect.

[0122] Test Example 3

[0123] The grape powdery mildew field efficacy test was carried out on the turmeric root stem extract emulsion prepared in Examples 1, 4-7.

[0124] (1) Test target and crop variety

[0125] Grape variety: Red Tip; Target: grape powdery mildew;

[0126] (2) Test agent, Examples 1, 4-7 were diluted with water by 500 times for standby;

[0127] Positive control agent, 325 grams / liter kresoxim-methyl SC, Zhejiang Tianfeng Biological Science Co., Ltd., registration certificate number: PD20172823, batch number: 20210318; diluted by 1000 times for standby; at the same time, set up water control.

[0128] (3) Test site: Zengjiaying, Binchuan, Dali, Yunnan

[0129] Plot arrangement: the test was set up 7 treatments, each treatment was repeated 4 times, and each plot repeated area was 15 square meters;

[0130] (4) Application method: Zhengzhou Xinxiu Agricultural Machinery Co., Ltd., 20 type knapsack electric sprayer was selected for application, and the agent was configured according to the test design, and the plants of each treatment were uniformly sprayed until small water droplets were formed;

[0131] The application was carried out at the early stage of disease (i.e. when there were sporadic lesions on the lower leaves of the plants), and the application was carried out twice with an interval of 8 days, and the application time was recorded;

[0132] (5) Investigation method:

[0133] Each treatment investigated 5 points, 2 plants per point, and all the leaves of each plant were investigated.

[0134] Disease grading standard:

[0135] 0 level: no lesion;

[0136] Grade 1: the area of disease spot accounts for less than 5% of the whole leaf area;

[0137] Grade 3: the area of disease spot accounts for 6%-10% of the whole leaf area;

[0138] Grade 5: the area of disease spot accounts for 11%-20% of the whole leaf area;

[0139] Grade 7: the area of disease spot accounts for 21%-40% of the whole leaf area;

[0140] Grade 9: the area of disease spot accounts for more than 40% of the whole leaf area.

[0141] (6) Calculation method of drug efficacy:

[0142] Disease index = (∑(number of leaves of each grade x relative grade value) / total number of leaves surveyed x 9) x 100

[0143] Preventive effect (%) = (1-(disease index of treatment area / disease index of control group)) x 100

[0144] The results are shown in Table 3:

[0145] Table 3

[0146]

[0147]

[0148] From the data results in Table 3, it can be seen that the curcuma root extract aqueous emulsion involved in the present application has excellent effect on preventing and treating grape powdery mildew, and the preparation process conditions of the curcuma root extract affect the prevention and treatment effect.

[0149] Test Example 4

[0150] The curcuma root stem extract aqueous emulsion prepared in Example 1 was subjected to field efficacy test of rose powdery mildew:

[0151] (1) Test target and crop variety

[0152] Rose variety: Su Xing; target: rose powdery mildew;

[0153] (2) Test agent, the product of Example 1 was diluted 500 times with water for standby;

[0154] Positive control agent, 25% ethirimol ME, Xi'an Modern Science and Technology Industry Co., Ltd., registration certificate number: PD20190149, batch number: 20201023018; diluted 1000 times for standby; a water control group was also set.

[0155] (3) Test site: Yiliang Goujie Town, Kunming, Yunnan

[0156] Cell arrangement: 2 treatments are set, each treatment has 4 cell repeats, and each cell repeat area is 15 square meters;

[0157] (4) Application method: 20 type knapsack electric sprayer of Zhengzhou Xinxiu Agricultural Machinery Co., Ltd. is selected, and the pesticide is configured according to the test design, and the plants of each treatment are uniformly sprayed until small water droplets are formed;

[0158] The pesticide is applied at the early stage of the disease (that is, when there are sporadic lesions on the lower leaves of the plants), the pesticide is applied once, and the application time is recorded;

[0159] (5) Investigation method:

[0160] Each treatment investigates 5 points, 2 plants per point, and all the leaves of each plant are investigated.

[0161] Disease grading standards:

[0162] 0 level: no lesion;

[0163] 1 level: the lesion area accounts for less than 5% of the entire leaf area;

[0164] 3 level: the lesion area accounts for 6%-10% of the entire leaf area;

[0165] 5 level: the lesion area accounts for 11%-20% of the entire leaf area;

[0166] 7 level: the lesion area accounts for 21%-40% of the entire leaf area;

[0167] 9 level: the lesion area accounts for more than 40% of the entire leaf area.

[0168] (6) Pesticide efficacy calculation method:

[0169] Disease index = (∑(number of leaves of each level x relative grade value) / total number of investigated leaves x 9) x 100

[0170] Preventive effect (%) = (1-(treatment area disease index / control group disease index)) x 100

[0171] The results are shown in Table 4:

[0172] Table 4

[0173] Group Control Positive control / Example 1 62.89％ 7 days after 1st application efficacy 74.52％

[0174] As can be seen from the data results in Table 4, the turmeric root extract emulsion involved in the present application has excellent effect on preventing and treating rose powdery mildew.

[0175] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate the present invention's aqueous emulsion of turmeric rhizome extract, its preparation method, and its application. However, the present invention is not limited to the above-mentioned embodiments, and it does not mean that the present invention must rely on the above-mentioned embodiments in order to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions for various raw materials in the present invention's product, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention.

[0176] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0177] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. An application of a turmeric rhizome extract aqueous emulsion in preventing and treating powdery mildew of roses, characterized in that: The components of the turmeric rhizome extract aqueous emulsion include, by weight percentage, 30-60% of turmeric rhizome extract, 5-15% of emulsifier, 1-10% of alcohol solvent, and 30-60% of water; The emulsifier includes a combination of nonionic emulsifier YUS-FS1 and anionic emulsifier YUS-A51G; The turmeric rhizome extract is prepared by a preparation method comprising the following steps: (1) drying and crushing the turmeric rhizome raw material, and then extracting it with a 65-85% ethanol aqueous solution to obtain an extract; (2) concentrating the extract and extracting with an organic solvent to obtain an aqueous phase product and an oil phase product; (3) molecularly distilling the oil phase product to remove turmeric oleoresin to obtain the turmeric rhizome extract.

2. The use of the turmeric rhizome extract aqueous emulsion in preventing and treating rose powdery mildew according to claim 1, characterized in that: The alcohol solvent includes any one of ethylene glycol, polyethylene glycol, propylene glycol, glycerol or glycerol, or a combination of at least two of them.

3. The use of the turmeric rhizome extract aqueous emulsion in preventing and treating rose powdery mildew according to claim 2, characterized in that: The alcohol solvent is a combination of ethylene glycol and glycerol.

4. The use of the turmeric rhizome extract aqueous emulsion in preventing and treating rose powdery mildew according to claim 1, characterized in that: The material-liquid ratio of the turmeric rhizome raw material to the ethanol aqueous solution is (30-70) g / L.

5. The use of the turmeric rhizome extract aqueous emulsion in preventing and treating rose powdery mildew according to claim 1, characterized in that: The extraction temperature is 15-30° C., and the extraction time is 30-50 hours.

6. The use of the turmeric rhizome extract aqueous emulsion in preventing and treating rose powdery mildew according to claim 1, characterized in that: The pressure condition of the molecular distillation is 0.1-5 Pa, and the temperature of the molecular distillation is 110-130°C.

7. The use of the turmeric rhizome extract aqueous emulsion in preventing and treating rose powdery mildew according to claim 1, characterized in that: The preparation method of the turmeric rhizome extract aqueous emulsion comprises: The emulsifier and water in a prescribed amount are mixed for the first emulsification, and then mixed with the turmeric rhizome extract and an alcohol solvent for the second emulsification to obtain the product.

8. The use of the turmeric rhizome extract aqueous emulsion in preventing and treating rose powdery mildew according to claim 7, characterized in that: The emulsification is carried out under stirring conditions, and the stirring speed is 3000-6000 rpm.

9. The use of the turmeric rhizome extract aqueous emulsion in preventing and treating rose powdery mildew according to claim 7, characterized in that: The time for the first emulsification and the second emulsification is independently 5-15 minutes.

10. A method for preventing and treating powdery mildew of roses, characterized in that: The control method comprises: applying an effective dose of the turmeric rhizome extract aqueous emulsion according to any one of claims 1 to 9 to the rose.

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