A high-quality and high-yield cultivation method of cordyceps sinensis

Abstract

The application discloses a high-quality and high-yield cultivation method of wasp pupa cordyceps, and belongs to the field of artificial cultivation technology of rare medicinal and edible fungi. The method selects frozen wasps at each day age of mature larva stage to metamorphic stage as main raw materials of culture medium, and the culture medium is comprehensive in nutrition and high in quality. The cordyceps cultivated by the method contains high medicinal and nutritional components such as cordycepin, amino acid and cordyceps polysaccharide, and the content of cordycepin is particularly outstanding. The application has high safety and is not affected by environmental factors. The application adopts a pipette injection method, and the culture medium is prepared through normal pressure sterilization or high pressure sterilization. The number of wasp pupae inoculated per hour is 20 times that of the prior art, and the bacterial contamination rate can reach 100%. The application solves the problems of labor-intensive, time-consuming and low bacterial contamination rate of the current manual injection method, shortens the time for the insect body to be fully covered with bacteria by more than one week, and increases the yield by more than 3 times. The application is convenient for large-scale production, and has novelty, creativity and wide application value.

Description

TECHNICAL FIELD

[0001] The application relates to a high-quality and high-yield Vespa pupa Cordyceps cultivation method and belongs to the field of artificial cultivation of rare medicinal and edible fungi. BACKGROUND

[0002] Ophiocordyceps sinensis is a kind of precious wild Chinese medicinal material in China, and has the effects of tonifying deficiency, benefiting essence, protecting the lung, benefiting the kidney and tonifying and invigorating the body. Due to the scarcity of wild Cordyceps resources and high price, it is difficult to benefit the public. In order to protect and develop its economic value and develop modern agriculture, in the 1980s, people developed and innovated Cordyceps militaris, and the main nutrients and medicinal components of the Cordyceps militaris are equivalent to those of wild Cordyceps. In 2009, Dan Yang Jiuxiancao Biotechnology Co., Ltd. scientific research personnel Lu Jiumei et al. used bee pupae as a Cordyceps culture medium, and through continuous practice, successfully cultivated bee pupae Cordyceps, and the authorization announcement number is (CN104054504 B).

[0003] The application broadens the Cordyceps cultivation field, provides an innovative Cordyceps cultivation method, and improves the content of functional components of Cordyceps. Since the bee pupae are small in size and tender in body, the application uses fresh mature bee pupae as the host, and in the cultivation process, it is easy to be infected with miscellaneous bacteria, leading to metamorphosis. In addition, the application adopts a manual injection method, and the culture medium is prepared through ozone sterilization, which is labor-consuming and time-consuming, has a low safety bacterial infection rate, and is not conducive to large-scale production. SUMMARY

[0004] The application provides a high-quality and high-yield Vespa pupa Cordyceps cultivation method to solve the problems in the prior art. Frozen Vespa mature larva stage to metamorphosis stage of each day old pupae (hereinafter referred to as Vespa pupae) are selected as the main raw material of the culture medium. Compared with bee pupae, the Vespa pupae are larger in size and more comprehensive in nutrition. The Vespa pupae not only contain high protein, low fat, various vitamins, amino acids and trace elements, but also contain multi-titanium, enzymes, soft phospholipids and other substances with high biological activity. The Vespa pupae can be used as green food and nutritional health products. The Vespa pupae not only play a regulating role in human metabolism and balance, but also promote the synthesis of cyclic adenosine monophosphate, protein helix structure and amino acid sequence normalization, so as to help the recovery of cell structure damaged by diseases such as tumors.

[0005] The Cordyceps cultivated by the application has higher contents of cordycepin, amino acids, Cordyceps polysaccharide, adenosine, SOD and other medicinal and nutritional components than wild Ophiocordyceps sinensis and bee pupae Cordyceps, especially the content of cordycepin.

[0006] In addition, the application has high safety and is not affected by environment and other factors; the application uses a pipette injection method, and the medium is prepared through normal pressure sterilization or high pressure sterilization, the number of wasp pupae inoculated per hour is 20 times that of the prior art, the bacterial infection rate can reach 100%, the time for the insect body to be full of bacteria is shortened by more than one week, the yield of wasp pupae Cordyceps is more than 3 times that of the prior art, and the application is convenient for large-scale production. The application has novelty, creativity and wide application value.

[0007] The application provides a high-quality and high-yield cultivation method of wasp pupae Cordyceps, and comprises the following steps:

[0008] (1) selecting wasp pupae as the pupae Cordyceps medium to be cultivated;

[0009] (2) preferably selecting a recently preserved pupae Cordyceps test tube original strain;

[0010] (3) preparation of a liquid pupae Cordyceps strain:

[0011] a formula of the liquid pupae Cordyceps strain: 15g of honey, 24g of pollen, 5g of proteose peptone, 0.7g of magnesium sulfate, 1.5g of potassium dihydrogen phosphate and 1000ml of water;

[0012] b preparation method: the above formula is placed in 1000ml of pure water, mixed and stirred, egg white is added and dispersed, and then filled into a glass measuring cup; 0.137MPa and 125℃ are adopted for sterilization for 40-45min, and then standing for 10-15h;

[0013] c the solution after standing is filtered and purified with 16 layers of sterile gauze, and then filled and sterilized for 40-45min;

[0014] d the preferred pupae Cordyceps test tube original strain is lighted and color changed for 36-48h, and then connected to the above c, and then standing for 36-48h in the dark at 17-23℃; and then cultured in the dark on a shaking bed at 17-23℃ and 155r / min for 5-7 days, and then taken out and placed in a refrigerator at 4℃ for standby;

[0015] (4) preparation of the medium: the wasp pupae are used as raw materials, and are divided and filled into culture bottles, 30-45g per bottle; high-temperature-resistant polypropylene film is used for sealing, normal pressure sterilization is adopted for 270-390min or high pressure sterilization is adopted for 40-45min, and then the culture bottles are cooled and placed in a sterile room for standby;

[0016] (5) inoculation and culture: the standby liquid strain is sprayed into the culture bottles by using a pipette, 2-4ml per bottle, and the liquid strain is sprayed 2-3 times per culture bottle, and then the culture bottles are sealed and placed in a culture room with a temperature of 16-22℃ and a humidity of 50-65% for dark culture;

[0017] (6) light culture:

[0018] The control conditions of the culture room are: 18-23℃, 1000-1300lx, humidity 70%-90%, continuous light culture for 36-48h;

[0019] b After the mycelium of the culture medium turns yellow, the temperature difference is increased for 36-72h;

[0020] (7) Grass stimulation: first, 4-6 holes are punched on the sealed plastic film as air holes using a medical disposable 5# syringe needle, the control conditions of the culture room during the grass stimulation stage are: light time 14-18h, culture temperature 20-25℃, humidity 70%-85%; dark light 6-10h at night, culture temperature 8-15℃, light and dark temperature difference 8-10℃, light is white light: 1000-1300lx, 2-4 days;

[0021] (8) When the Cordyceps militaris fruiting body grows to 0.5-1cm high, water is supplemented in time and in appropriate amount, the light intensity is adjusted to 300-400lx, the culture temperature is 18-24℃, the humidity is 70%-85%, and the light is seen for 10-16h per day;

[0022] (9) Harvesting: when the Cordyceps militaris fruiting body grows to 5.5-8cm, the whole Cordyceps militaris can be harvested, after harvesting, it is aired for 1-2 days, then it is dried at a temperature of 45-65℃, until the whole Cordyceps militaris contains water less than 8%, and then it is stored in a refrigerator for freezing.

[0023] Preferably, in step (1), the wasp pupae are selected as: frozen wasp pupae of each age from mature larva stage to metamorphosis stage;

[0024] Preferably, in step (2), the Cordyceps militaris test tube original strain stored recently is selected, and is exposed to light within 48h before inoculation, and the one with better color change is selected as standby;

[0025] Preferably, in step (3)a, 15g of pure natural mature honey, 12g of Chinese milk vetch pollen and 12g of tea pollen are selected; 5g of proteose peptone, 0.7g of magnesium sulfate, 1.5g of potassium dihydrogen phosphate and 1000ml of water are selected;

[0026] Preferably, in step (3)b, the formula in step (3)a is put into 1000ml of pure water, mixed and stirred, 3 egg whites are added and fully stirred, filled into a 2000ml glass measuring cup, sterilized at 0.137MPa and 125℃ for 40-45min, and then left for 10-15h;

[0027] Preferably, in step (3)c, the solution after standing is filtered and purified with sixteen sterile gauze, and then respectively filled into five 500ml triangular bottles, and continues to be sterilized at 0.137MPa and 125℃ for 40-45min, and when cooled to 95-85℃, it is quickly taken out and sent to the inoculation room;

[0028] Preferably, in step (3)d, the preferred Cordyceps militaris test tube original strain is lighted for 36-48h, the outer wall of the test tube is wiped with 75% alcohol for disinfection, and then 3-5 pieces of 0.2*0.3mm mycelium pieces are picked from the test tube with a transplant shovel and inoculated into step (3)c in a sterile room on a super-clean workbench, and then cultured at 17-23℃ for 36-48h in the dark, and then cultured at 17-23℃ and 155r / min in the dark for 5-7 days, and then taken out and placed in a 4℃ refrigerator for standby when the liquid culture medium is covered with mycelium;

[0029] Preferably, in step (4), hornet pupae are used as raw materials, and are divided and placed in culture bottles with a height of 10cm and a diameter of 7cm, 30-45g per bottle. The culture bottles are sealed with high-temperature-resistant polypropylene film, and are sterilized at normal pressure for 240-390min or at high pressure for 40-45min, and then cooled and placed in a sterile room for standby;

[0030] Preferably, in step (5), the standby liquid strain is injected into the culture bottle using a pipette gun, 2-4mL per bottle, and the liquid strain is sprayed 2-3 times in each culture bottle, and then placed in a culture room with a temperature of 16-22℃ and a humidity of 50-65% for dark culture for 9-13 days after sealing;

[0031] Preferably, in step (6)a, the control conditions of the culture room are 18-23℃, white light illumination 1000-1300lx, and humidity 70%-90%, and the illumination is continued for 36-48h until the yellow color is seen;

[0032] Preferably, in step (6)b, after the mycelium of the culture medium turns yellow, the temperature difference is increased for 36-72h, and then the culture is continued after sealing;

[0033] Preferably, in step (7), 4-6 holes are first punched on the sealed plastic film as air holes with a medical disposable 5# syringe needle, the control conditions of the culture room during the grass stimulation stage are illumination time 14-16h, culture temperature 20-25℃, and humidity 70%-85%; dark light 6-10h at night, culture temperature 8-15℃, and the temperature difference between light and dark is 8-10℃, and the air is ventilated appropriately every day to ensure fresh air, and the light is white light illumination: 1000-1300lx, 2-4 days;

[0034] Preferably, in step (8), when the Cordyceps militaris fruiting body is grown to a height of 0.5-1 cm, water is supplemented in time and in an appropriate amount, the light intensity is adjusted to 300-400 lx, the culture temperature is 18-24℃, the humidity is 70%-85%, the light exposure is 10-16 h per day, and the culture time is 35-50 days.

[0035] Preferably, in step (9), the dried Osmia bumblebee Cordyceps militaris is cooled, sorted, packaged, and stored, the sorting is performed according to shape, size, and quality, and the storage conditions are: storage in the dark and at a temperature below -5℃.

[0036] Advantages

[0037] Compared with the prior art, the present application has the following advantages due to the adoption of the above technical solutions:

[0038] 1. The present application uses frozen Osmia bumblebee pupae as the main raw material for the culture medium, and the culture medium is prepared by normal pressure sterilization or high pressure sterilization, so the safety performance is high and the culture medium is not affected by environmental factors.

[0039] 2. The present application uses a pipette jet method, and the number of Osmia bumblebee pupae inoculated per hour is 20 times that of the prior art, the bacterial infection rate can reach 100%, the time for the insect body to be fully covered with bacteria is shortened by more than one week, and the yield of Cordyceps militaris from Osmia bumblebee pupae is more than 3 times that of the prior art, which is conducive to large-scale production.

[0040] 3. Dan Yang Jiuxiancao Biotechnology Co., Ltd. has conducted a comprehensive analysis of the nutritional components of Cordyceps militaris fruiting bodies. The results show that the main nutritional components of Cordyceps militaris fruiting bodies obtained by cultivating different pupae as culture media are very different, and the main nutritional components of Cordyceps militaris obtained by using Osmia bumblebee pupae as the culture medium are higher than the contents of wild Cordyceps militaris and bee pupae Cordyceps militaris, especially the content of cordycepin, which is 1.5 times that of bee pupae Cordyceps militaris and 50-100 times that of wild Cordyceps militaris, and a breakthrough has been made in the field of Cordyceps militaris.

[0041] 4. Osmia bumblebees are insectivorous insects that can naturally suppress the rampant of agricultural and forestry pests and reduce environmental pollution caused by pesticides, and have good economic and ecological benefits. In Yunnan, Guizhou, Sichuan, and Hunan, Osmia bumblebee larvae and pupae are the most common edible insects. With the continuous development of science and technology, Osmia bumblebee pupae have achieved large-scale production, bringing certain economic benefits to local farmers. However, it is regrettable that because Osmia bumblebee pupae have a high protein content, many people can easily have an allergic reaction after eating them, and Osmia bumblebee pupae deteriorate quickly and are not easy to store, so local farmers can only cultivate a small amount of Osmia bumblebee pupae to meet the needs of a small number of consumers.

[0042] The present application can convert the hornet pupa with super protein nutrition and easy to cause allergy into a high-quality, old and young suitable medicinal edible fungus Cordyceps militaris. The present application can not only contribute to human health, but also create economic, ecological and social benefits for the local agriculture, forestry, animal husbandry and sideline industries. DETAILED DESCRIPTION

[0043] The present application will be further described in detail below in combination with specific examples. It should be understood that these examples are used to illustrate the basic principles, main features and advantages of the present application, and the present application is not limited in scope by the following examples. The implementation conditions used in the examples can be further adjusted according to specific requirements, and the implementation conditions not mentioned are usually the conditions in the conventional experiments.

[0044] Example 1

[0045] A high-quality and high-yield hornet pupa Cordyceps militaris cultivation method is provided, which comprises the following steps:

[0046] (1) Selecting 17-day-old frozen hornet pupae as the culture medium of the Cordyceps militaris to be cultivated;

[0047] (2) Preferably, the Cordyceps militaris test tube original seed preserved recently;

[0048] (3) Preparation of liquid Cordyceps militaris seed:

[0049] a. Formula of liquid Cordyceps militaris seed: honey 15g, pollen 24g, protein peptone 5g, magnesium sulfate 0.7g, potassium dihydrogen phosphate 1.5g, water 1000ml;

[0050] b. Preparation method: Put the above formula into 1000ml pure water, mix and adjust, then add egg white to disperse, fill into a glass measuring cup, sterilize at 0.137MPa and 125℃ for 40-45min, and then stand for 10-15 hours;

[0051] c. After standing, the solution is filtered and purified with sixteen layers of sterile gauze, then filled and sterilized for 40-45min;

[0052] d. The preferred Cordyceps militaris test tube original seed is lightened for 36-48h, then inoculated into the above c, and cultured at 17-23℃ in the dark for 36-48h, and cultured in the dark at 17-23℃ and 155r / min for 5-7 days. When the liquid culture medium is covered with mycelium, it is taken out and placed in a 4℃ refrigerator for standby;

[0053] (4) Preparation of culture medium: 17-day-old hornet pupae are used as raw materials and are divided into culture bottles, 30-45g per bottle. High-temperature resistant polypropylene film is used for sealing, and normal pressure sterilization is used for 270-390min or high pressure sterilization for 40-45min. After cooling, it is placed in a sterile room for standby;

[0054] (5) Inoculation and culture: the standby liquid strain is sprayed into the culture bottle by using a pipette, 2-4 mL of the liquid strain is inoculated into each bottle, and the liquid strain is sprayed 2-3 times in each culture bottle. After being sealed, the culture bottles are placed in a culture room with a temperature of 16-22°C and a humidity of 50-65% for dark culture;

[0055] (6) Light culture:

[0056] a The control conditions of the culture room are as follows: 18-23°C, 1000-1300 lx, and a humidity of 70%-90%, and the light culture lasts for 36-48 h;

[0057] b After the mycelium of the culture medium turns yellow, the temperature difference is increased to stimulate for 36-72 h;

[0058] (7) Grass stimulation: 4-6 holes are punched on the sealed plastic film as air holes by using a medical disposable 5# syringe needle. The control conditions of the culture room during the grass stimulation stage are as follows: light time of 14-18 h, culture temperature of 20-25°C, and humidity of 70%-85%; dark light of 6-10 h at night, culture temperature of 8-15°C, light and dark temperature difference of 8-10°C, and white light illumination of 1000-1300 lx, 2-4 days;

[0059] (8) When the Cordyceps militaris fruiting body grows to a height of 0.5-1 cm, water is supplemented in time and in an appropriate amount, the light intensity is adjusted to 300-400 lx, the culture temperature is adjusted to 18-24°C, and the humidity is adjusted to 70%-85%, and the light is on for 10-16 h per day;

[0060] (9) Harvesting: when the Cordyceps militaris fruiting body grows to a height of 5.5-8 cm, the whole Cordyceps militaris can be harvested. After harvesting, the Cordyceps militaris is aired for 1-2 days, and then dried at a temperature of 45-65°C until the whole Cordyceps militaris has a water content of less than 8%, and then stored in a refrigerator for freezing.

[0061] Example 2

[0062] A high-quality and high-yield cultivation method of Cordyceps militaris is provided, which comprises the following steps:

[0063] (1) A 22-day-old frozen Cordyceps militaris wasp is selected as the culture medium of the Cordyceps militaris to be cultivated;

[0064] (2) The Cordyceps militaris test tube original strain stored in recent period is preferably selected;

[0065] (3) Preparation of liquid Cordyceps militaris strain:

[0066] a The formula of the liquid Cordyceps militaris strain is as follows: honey 15 g, pollen 24 g, protein peptone 5 g, magnesium sulfate 0.7 g, potassium dihydrogen phosphate 1.5 g, and water 1000 ml;

[0067] b Preparation method: Put the above formula into 1000 ml of pure water, mix and homogenize, then add egg white and disperse, fill into a glass measuring cup, sterilize at 0.137 MPa and 125°C for 40-45 min, and then stand for 10-15 hours;

[0068] c The solution after standing is filtered and purified with 16 layers of sterile gauze, then filled and sterilized for 40-45 min;

[0069] d The preferred Cordyceps militaris test tube original strain is lighted for 36-48 hours to change color, then inoculated into the above c, and cultured at 17-23°C in the dark for 36-48 hours, and cultured in the dark on a shaking bed at a temperature of 17-23°C and a speed of 155 r / min for 5-7 days, and then taken out and placed in a refrigerator at 4°C for standby use when the liquid culture medium is covered with mycelium;

[0070] (4) Preparation of culture medium: 22-month-old wasp pupae are used as raw materials and are divided into culture bottles, 30-45 g per bottle. High-temperature resistant polypropylene film is used for sealing, and normal pressure sterilization is used for 270-390 min or high pressure sterilization for 40-45 min, and then cooled and placed in a sterile room for standby use;

[0071] (5) Inoculation and culture: the standby liquid strain is sprayed into the culture bottle using a pipette gun, 2-4 mL per bottle, and the liquid strain is sprayed 2-3 times per culture bottle, and then placed in a culture room with a temperature of 16-22°C and a humidity of 50-65% for dark culture after sealing;

[0072] (6) Light culture:

[0073] a The control conditions of the culture room are 18-23°C, 1000-1300 lx, and a humidity of 70%-90%, and the light culture lasts for 36-48 hours;

[0074] b After the mycelium of the culture medium turns yellow, the temperature difference is increased for 36-72 hours;

[0075] (7) Grass stimulation: first, 4-6 holes are made on the sealed plastic film as air holes using a medical disposable 5# syringe needle, and the control conditions of the culture room during the grass stimulation stage are 14-18 hours of light time, a culture temperature of 20-25°C, and a humidity of 70%-85%; 6-10 hours of dark light at night, a culture temperature of 8-15°C, a light and dark temperature difference of 8-10°C, and white light with an intensity of 1000-1300 lx for 2-4 days;

[0076] (8) When the Cordyceps militaris fruiting body grows to a height of 0.5-1 cm, water is added in time and in an appropriate amount, the light intensity is adjusted to 300-400 lx, the culture temperature is adjusted to 18-24°C, the humidity is adjusted to 70%-85%, and the light time is adjusted to 10-16 hours per day;

[0077] (9) Harvesting: when the Cordyceps militaris fruiting bodies grow to 5.5-8 cm, the whole Cordyceps militaris can be harvested, and after being dried for 1-2 days, the whole Cordyceps militaris is dried at a temperature of 45-65 °C until the water content of the whole Cordyceps militaris is less than 8%, and then stored in a refrigerator for freezing.

[0078] Comparative Example 1:

[0079] The important difference from Examples 1-2 is that fresh mature bee pupae are used as hosts, and the culture medium is prepared by ozone sterilization using a manual injection method.

[0080]

[0081] As shown in the above table, the number of inoculations per hour is 20 times that of the prior art, the Cordyceps militaris infection rate can reach 100%, the time for the insect body to be full of fungus is shortened by more than one week, and the Cordyceps militaris yield is more than 3 times that of the prior art, which is conducive to large-scale production. The Cordyceps militaris cultivated by the present application contains cordycepin, polysaccharides, amino acids, etc., which are higher than the contents of wild Cordyceps militaris and bee pupae Cordyceps militaris, especially the content of cordycepin, which is 1.5 times that of bee pupae Cordyceps militaris and 50-100 times that of wild Cordyceps militaris.

[0082] Finally, it should be pointed out that the above description is only a preferred embodiment of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent replacements to some technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A method for cultivating high-quality and high-yield Cordyceps militaris, characterized in that, Comprise the following steps: (1) select hornet pupae as the to be cultivated Cordyceps militaris culture medium; select hornet pupae for: frozen hornet mature larval stage to the metamorphic stage of each age pupae; (2) select the recently preserved Cordyceps militaris test tube original species; (3) preparation of liquid Cordyceps militaris strain: a formula of liquid Cordyceps militaris strain: honey 15g, pollen 24g, protein peptone 5g, magnesium sulfate 0.7g, potassium dihydrogen phosphate 1.5g, water 1000ml; b preparation method: put the above formula into 1000ml pure water, mix and adjust, then add egg white to scatter, fill into glass measuring cup, sterilize at 0.137MPa, 125℃ for 40-45min, then stand for 10-15h; c the solution after standing, filter and purify with sixteen sterile gauze, then continue to sterilize for 40-45min after filling; d the Cordyceps militaris test tube original species is lighted for 36-48h, then is connected to the above c, and is cultured at 17-23℃ for 36-48h in the dark, and is cultured in the dark on a shaking table at 155r / min and 17-23℃ for 5-7d, and is taken out and placed in a refrigerator at 4℃ after the liquid culture medium is covered with mycelium; (4) preparation of culture medium: use hornet pupae as raw material, and divide and pack in culture bottles, 30-45g per bottle; use high-temperature resistant polypropylene film to seal, and sterilize at atmospheric pressure for 270-390min or at high pressure for 40-45min, and then cool and place in a sterile room for standby; (5) inoculation and culture: use a pipette gun to spray the standby liquid strain into the culture bottle, 2-4mL per bottle, spray 2-3 times of liquid strain in each culture bottle, seal, and place in a culture room at 16-22℃ and 50-65% humidity to culture in the dark; (6) light culture: a control conditions of the culture room: 18-23℃, 1000-1300lx, and 70%-90% humidity, for 36-48h light culture; b after the mycelium of the culture medium turns yellow, stimulate for 36-72h by increasing the temperature difference; (7) grass stimulation: first, use a medical disposable 5# syringe needle to pierce 4-6 holes on the sealed plastic film as air holes, and control conditions of the culture room during the grass stimulation stage: light time 14-18h, culture temperature 20-25℃, and humidity 70%-85%; dark light 6-10h at night, culture temperature 8-15℃, light and dark temperature difference 8-10℃, and light 1000-1300lx, 2-4d; (8) when the Cordyceps militaris fruiting body grows to 0.5-1cm high, timely and appropriately supplement water, adjust light intensity to 300-400lx, culture temperature to 18-24℃, and humidity to 70%-85%, and expose to light for 10-16h per day; (9) harvesting: when the Cordyceps militaris fruiting body grows to 5.5-8cm, harvest the whole Cordyceps militaris, air for 1-2d after harvesting, then dry at 45-65℃, until the whole Cordyceps militaris contains less than 8% water, and then store in a refrigerator and freeze.

2. The cultivation method of the hornet cocooned C. formosana according to claim 1, characterized in that: In step (3)a, 15g of pure natural mature honey, 12g of Chinese milk vetch pollen, 12g of tea pollen, 5g of proteose peptone, 0.7g of magnesium sulfate, 1.5g of potassium dihydrogen phosphate and 1000ml of water are selected; in step (3)b, the formula in step (3)a is put into 1000ml of pure water, mixed and stirred, 3 egg whites are added and stirred uniformly, filled into 2000ml glass measuring cups, sterilized at 0.137MPa and 125℃ for 40-45min, and then left to stand for 10-15h; in step (3)c, the solution after standing is filtered and purified with 16 layers of sterile gauze, and then filled into 5 500ml triangular bottles, continues to be sterilized at 0.137MPa and 125℃ for 40-45min, and then quickly taken out when cooled to 95-85℃ and sent to the inoculation room; in step (3)d, the Cordyceps militaris test tube original strain is lighted for 36-48h, the outer wall of the test tube is disinfected with 75% alcohol, and then 3-5 pieces of 0.2x0.3mm mycelium pieces are picked up from the test tube with a transplanting shovel and inoculated into step (3)c in a sterile room super-clean workbench, left to stand and cultivate in the dark at 17-23℃ for 36-48h, and then cultivated in the dark on a shaking bed at a temperature of 17-23℃ and a speed of 155r / min for 5-7d, and then taken out and placed in a 4℃ refrigerator for standby use after the liquid culture medium is covered with mycelium.

3. The cultivation method of the hornet cocooned C. formosana according to claim 1, characterized in that: In step (4), hornet pupae are used as raw materials, and are divided and filled into culture bottles with a height of 10cm and a diameter of 7cm, 30-45g per bottle; the bottles are sealed with high-temperature resistant polypropylene film, and sterilized at normal pressure for 240-390min or at high pressure for 40-45min, and then cooled and placed in a sterile room for standby use.

4. The cultivation method of the hornet cocooned C. formosana according to claim 1, characterized in that: In step (5), the standby liquid strain is injected into the culture bottles using a transfer gun, 2-4mL per bottle, and the liquid strain is sprayed 2-3 times in each culture bottle, and then placed in a culture room with a temperature of 16-22℃ and a humidity of 50-65% for dark cultivation for 9-13d.

5. The cultivation method of the hornet cocooned C. formosana according to claim 1, characterized in that: In step (6)a, the control conditions of the culture room are as follows: 18-23℃, white light illumination of 1000-1300lx, and a humidity of 70%-90%, and the illumination is continued for 36-48h until the color is yellow; in step (6)b, after the mycelium of the culture medium turns yellow, the temperature difference is increased to stimulate for 36-72h, and then the culture is continued after sealing.

6. The cultivation method of the hornet cocooned C. formosana according to claim 1, characterized in that: In step (7), 4-6 holes are first punched on the sealed plastic film as air holes using a medical disposable 5# syringe needle, the control conditions of the culture room during the grass stimulation stage are as follows: illumination time of 14-16h, culture temperature of 20-25℃, and humidity of 70%-85%; dark light for 6-10h at night, culture temperature of 8-15℃, and a temperature difference of 8-10℃ between light and dark, and the air is ventilated appropriately every day to ensure fresh air, and the light is white light illumination of 1000-1300lx for 2-4d.

7. The cultivation method of the hornet cocooned C. formosana according to claim 1, characterized in that: In step (8), when the Cordyceps militaris fruiting bodies grow to 0.5-1 cm high, water is supplemented in time and in proper amount, the light intensity is adjusted to 300-400 lx, the culture temperature is 18-24℃, the humidity is 70%-85%, the light is on for 10-16 hours per day, and the culture time is 35-50 days.

8. The cultivation method of the hornet cocooned C. formosana according to claim 1, characterized in that: In step (9), the dried Cordyceps militaris is cooled, picked, packaged and stored. During picking, it is packaged according to shape, size and quality. The storage condition is: light-proof storage at a temperature below -5℃.

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