A high-efficiency compound nematicide for pine wood nematode disease and preparation method thereof

Abstract

The present application relates to the technical field of nematicides for pine wood nematode disease, and specifically discloses a highly efficient compound nematicide for pine wood nematode disease and a preparation method thereof. The components of the compound nematicide include nematicides and adjuvants, the adjuvants include synergists, surfactants, solvents and rejuvenation agents, the nematicide is a mixture of emamectin benzoate and rudol, the synergist is piperonyl butoxide, the solvent includes DMF and chloride solution, and the rejuvenation agent includes amino oligosaccharide aqueous solution; in the compound nematicide, the mass fraction of rudol is 10-20%, the mass fraction of emamectin benzoate is 0.5-3.5%, and the mass fraction of the rejuvenation agent is 2-8%. The compound nematicide of the present application can also have a significant killing effect on nematodes that have developed emamectin benzoate resistance, and the selection of rejuvenation agents reduces the nematode's gnawing on pine trees, promotes the repair of internal damage to pine trees, and fully realizes the treatment of pine trees infected with pine wood nematode disease.

Description

Technical Field

[0001] The present application relates to the technical field of nematicides for pine wood nematode disease, and more specifically, to a highly effective compound nematicide for pine wood nematode disease and a preparation method thereof. Background Art

[0002] Pine nematodes are the pathogens that cause pine wilt disease, and are usually transmitted by Monochamus alternatus. When Monochamus alternatus feeds on pine twigs, pine nematodes take the opportunity to enter the pine tree through the feeding wounds, and can spread to various parts of the tree, including the trunk, roots, and leaves, in about 40 days. Pine wilt disease is highly contagious, and pine trees will become severely weakened and lose their economic value after being infected. Therefore, pine wilt disease is a major threat to forestry production.

[0003] In the related art, there is a nematicide for pine wood nematode disease, the components of which include a nematicide and an adjuvant, the nematicide is emamectin benzoate, the adjuvant includes a synergist, a defoamer, a surfactant and a solvent, the synergist is piperonyl butoxide and sodium chloride, and the solvent is prepared by mixing DMF and water in a weight ratio of 1:4. In the nematicide, the mass fraction of emamectin benzoate is 0.5-3.5%.

[0004] With respect to the above-mentioned related technologies, the inventors believe that although the nematicides in the related technologies have a certain killing effect on nematodes, their main purpose is to prevent the occurrence of nematode diseases. For pine trees that have already shown symptoms of nematode diseases, even if the nematodes are killed by applying the nematicides, it is difficult to make up for the damage caused by the nematodes gnawing on the pine trees. Moreover, the widespread use of emamectin benzoate has made nematodes in some areas resistant to emamectin benzoate. The emergence of resistant nematodes will make it more difficult for the nematicides in the related technologies to achieve an ideal treatment effect. Summary of the invention

[0005] Even if the nematicides in the related art kill nematodes, it is difficult to make up for the damage caused by the nematodes to the pine trees, and the emergence of drug-resistant nematodes will make it more difficult for the nematicides to achieve the ideal treatment effect, limiting the practical application of the nematicides. In order to improve this defect, the present application provides a high-efficiency compound nematicide for pine wood nematode disease and a preparation method thereof.

[0006] In the first aspect, the present application provides a highly effective compound nematicide for pine wood nematode disease, adopting the following technical scheme: a highly effective compound nematicide for pine wood nematode disease, the components of the compound nematicide including a nematicide and an adjuvant, the adjuvant including a synergist, a surfactant, a solvent and a rejuvenating agent, the nematicide being a mixture of emamectin benzoate and lufudan, the synergist being piperonyl butoxide, the solvent including DMF and a chloride solution, the rejuvenating agent including an amino oligosaccharide aqueous solution; in the compound nematicide, the mass fraction of lufudan is 10-20%, the mass fraction of emamectin benzoate is 0.5-3.5%, and the mass fraction of the rejuvenating agent is 2-8%.

[0007] By adopting the above technical scheme, the present application compounds emamectin benzoate with rudol, and on this basis, adds a rejuvenation agent to the formula system of the nematicide. Emamectin benzoate and rudol have a synergistic effect, and can also have a significant killing effect on nematodes that have developed emamectin benzoate tolerance. As a rejuvenation agent, amino oligosaccharides aqueous solution can promote the growth of pine trees, thereby repairing the damage caused by nematode gnawing. While promoting plant growth, amino oligosaccharides can also promote plants to secrete disease-resistant proteins such as chitinase, glucanase and PR protein, which can kill pathogenic fungi such as blue-staining bacteria that grow in pine trees. There is a symbiotic relationship between pathogenic fungi such as blue-staining bacteria and pine wood nematodes. The death of pathogenic fungi inhibits the metabolism of pine wood nematodes, which helps to reduce the gnawing of pine trees by nematodes. Therefore, after applying the compound nematicide of the present application to the forest area that has been invaded by nematodes and the nematodes are resistant to emamectin benzoate, it can exert a more ideal therapeutic effect than the nematicides in the related art.

[0008] Preferably, in the compound nematicide, the mass fraction of Lufuda is 12-18%, the mass fraction of emamectin benzoate is 1.0-3.0%, and the mass fraction of the rejuvenation agent is 4-6%.

[0009] By adopting the above technical scheme, the dosage of Lufuda, emamectin benzoate and rejuvenation agent was optimized, and the diseased pine trees were fully rescued under the premise of saving the dosage of raw materials.

[0010] Preferably, the solute in the chloride salt solution is potassium chloride or zinc chloride.

[0011] By adopting the above technical scheme, the insecticidal principle of emamectin benzoate is to promote the penetration of chloride ions into the nerve cells of nematodes, so that the nematodes die due to nerve cell disorder. After the chloride solution penetrates into the trunk, a large amount of chloride ions are provided for this insecticidal process, which is conducive to the insecticidal effect of emamectin benzoate. Although potassium chloride and zinc chloride can both provide chloride ions, zinc chloride can also promote the swelling of cellulose. The occurrence of swelling increases the spacing between the cellulose and hemicellulose molecular chains in the wood, which is conducive to the penetration of the compound nematicide.

[0012] Preferably, the chloride salt solution is a potassium chloride solution, and the components of the auxiliary agent also include a permeabilizing agent, and the permeabilizing agent is polyethylene glycol, and the polyethylene glycol is liquid at a standard atmospheric pressure of 20°C.

[0013] By adopting the above technical solution, the present application uses polyethylene glycol as a penetrant. Polyethylene glycol can dissolve lignin at room temperature, which is beneficial for the full penetration of the compound nematicide in the tree trunk.

[0014] Preferably, the mass fraction of the penetrant in the compound nematicide is 5-15%.

[0015] By adopting the above technical solution, the dosage of the penetrant when polyethylene glycol is used is optimized, which helps to fully improve the penetration of the compound nematicide in the tree trunk.

[0016] Preferably, the chloride salt solution is a zinc chloride solution with a solute mass fraction of 4-8%.

[0017] By adopting the above technical solution, although zinc chloride can improve the penetration effect of the compound nematicide by promoting swelling, zinc ion is a good ligand and is prone to complexation reaction. When the zinc ion concentration is too high, zinc ions are complexed with hydroxyl, carbonyl and other groups in the tree trunk to form a large amount of flocculent insoluble matter, which will block the penetration channel of the compound nematicide and affect the penetration effect of the compound nematicide. After optimization, when the chloride salt solution is a zinc chloride solution with a solute mass fraction of 4-8%, the penetration effect of the compound nematicide is relatively good.

[0018] Preferably, the chloride salt solution is a zinc chloride solution with a solute mass fraction of 10%, and the components of the auxiliary agent also include a penetrant, and the penetrant is lactic acid.

[0019] By adopting the above technical solution, in the compound nematicide, lactic acid can be complexed with zinc ions in advance to form complex chloride salt. The complex chloride salt can still promote the swelling of cellulose, and lactic acid has occupied the complexation site, so the complex chloride salt is hindered from complexing with hydroxyl, carbonyl and other groups in the trunk, and the degree of flocculation is small, which helps to improve the penetration effect of the compound nematicide.

[0020] Preferably, the molar ratio between the lactic acid and the zinc chloride in the chloride salt solution is 1:(2-4).

[0021] By adopting the above technical solution, the dosage of lactic acid as the penetrant is optimized, and the penetration effect of the compound nematicide is fully improved under the premise of saving raw materials.

[0022] Preferably, the amino oligosaccharide aqueous solution is prepared according to the following method:

[0023] (1) adding acid-soluble chitosan to the lignite extract, stirring and dissolving, and obtaining liquid A;

[0024] (2) heating solution A to 50-60°C, diluting it with water, and then keeping the temperature to react for 4-5 hours to obtain solution B;

[0025] (3) Separate liquid B at 4000 rpm for 4 minutes and take the supernatant, which is the amino oligosaccharide aqueous solution.

[0026] By adopting the above technical scheme, the present application uses acid-soluble chitosan as a raw material, and obtains an amino oligosaccharide aqueous solution that can be used as a rejuvenation agent through a reaction with a lignite extract.

[0027] In a second aspect, the present application provides a method for preparing a highly effective compound nematicide for pine wood nematode disease, using the following technical solution.

[0028] A method for preparing a highly effective compound nematicide for pine wood nematode disease comprises the following steps:

[0029] (1) mixing emamectin benzoate and rufuda uniformly to obtain a nematicide; and setting aside;

[0030] (2) The agent and any of the above-mentioned auxiliary agents are mixed and stirred evenly to obtain a highly effective compound nematicide for pine wood nematode disease.

[0031] By adopting the above technical scheme, the method of the present application first prepares the nematicide, and then compound the nematicide with an auxiliary agent to obtain a highly effective compound nematicide for pine wood nematode disease.

[0032] In summary, this application has the following beneficial effects:

[0033] 1. The compound nematicide of the present application can also play a significant killing effect on nematodes that have developed emamectin benzoate resistance through the compounding of emamectin benzoate and Lufuda. As a rejuvenation agent, the amino oligosaccharide aqueous solution can promote the growth of pine trees and repair the damage caused by nematode gnawing. In addition, the amino oligosaccharide aqueous solution also induces pine trees to produce an immune response, promotes the death of pathogenic fungi that coexist with pine wood nematodes, inhibits the metabolism of pine wood nematodes, and helps reduce the gnawing of pine trees by nematodes.

[0034] 2. In the present application, the chloride salt solution is preferably a zinc chloride solution with a solute mass fraction of 10%, and lactic acid is complexed with zinc ions in advance. After lactic acid occupies the complexing site of zinc ions, the nematicide has less flocculation during the penetration process, and can fully swell the cellulose in the pine wood xylem, which is conducive to the full penetration of the compound nematicide. DETAILED DESCRIPTION

[0035] The present application is further described in detail below in conjunction with embodiments, preparation examples and comparative examples. The raw materials involved in the present application can all be obtained commercially.

[0036] Preparation example of amino oligosaccharide aqueous solution

[0037] The following is an explanation using Preparation Example 1.

[0038] Preparation Example 1

[0039] In this preparation example, the amino oligosaccharide aqueous solution was prepared according to the following method:

[0040] (1) Add 50 g of acid-soluble chitosan to 7 kg of lignite extract and stir until the acid-soluble chitosan is completely dissolved to obtain liquid A;

[0041] (2) Liquid A was heated to 50°C, diluted with 1 kg of water, and then kept warm for 4 hours to obtain Liquid B;

[0042] (3) Separate liquid B at 4000 rpm for 4 minutes and take the supernatant, which is the amino oligosaccharide aqueous solution.

[0043] Example

[0044] Examples 1-5

[0045] The following description is given by taking Example 1 as an example.

[0046] Example 1

[0047] In this embodiment, the high-efficiency compound nematicide for pine wood nematode disease is compounded by a nematicide and an adjuvant, the nematicides are emamectin benzoate and rufuta, and the adjuvants are synergists, surfactants, solvents and rejuvenation agents. The mass fractions of the components in the compound nematicide are as follows: emamectin benzoate 0.5%, rufuta 10%, synergist 0.4%, surfactant 20%, rejuvenation agent 2%, defoamer 7%, and the rest is supplemented to 100% by solvent; the synergist is piperonyl butoxide, the surfactant is Tween 60, the rejuvenation agent is the amino oligosaccharide aqueous solution of Preparation Example 1, the defoamer is a silicone defoamer, and the solvent is a mixture of DMF and a chloride solution in a weight ratio of 1:4; the chloride solution is a potassium chloride solution, and in the chloride solution, the mass fraction of the solute potassium chloride is 3.3%.

[0048] In this embodiment, the highly effective compound nematicide for pine wood nematode disease is prepared according to the following steps:

[0049] (1) mixing emamectin benzoate and rufuda uniformly to obtain a nematicide; and setting aside;

[0050] (2) The auxiliary agents are mixed and stirred evenly to obtain a highly effective compound nematicide for pine wood nematode disease.

[0051] As shown in Table 1, the difference between Examples 1-5 is mainly that the raw material ratios of the compound nematicide are different.

[0052] Table 1 Ratio of raw materials of compound nematicide

[0053]

[0054]

[0055] Example 6

[0056] The difference between this embodiment and embodiment 3 is that the auxiliary agent further comprises a penetrant, and the penetrant is polyethylene glycol (PEG-200). In the compound nematicide of this embodiment, the dosage ratio of each component except the penetrant is the same as that of embodiment 3.

[0057] In the compound nematicide of this embodiment, the mass fraction of the penetrant is 3.0%.

[0058] As shown in Table 2, the difference between Examples 6-10 is that, in the compound nematicide, the mass fractions of the penetrant and the solvent are different, while the usage ratios of the components other than the penetrant are the same as that of Example 3.

[0059] Table 2 Mass fraction of penetrant and solvent

[0060] sample Example 6 Example 7 Example 8 Example 9 Example 10 Penetrant / % 3.0 5.0 10.0 15.0 18.0

[0061] Embodiment 11

[0062] The difference between this embodiment and embodiment 3 is that the chloride salt solution is a zinc chloride solution with a solute mass fraction of 3%.

[0063] As shown in Table 3, the difference between Examples 11-15 is that the solute mass fraction of the zinc chloride solution (hereinafter referred to as zinc chloride concentration) is different.

[0064] Table 3 Solute mass fraction in zinc chloride solution

[0065] sample Embodiment 11 Example 12 Embodiment 13 Embodiment 14 Embodiment 15 Zinc chloride concentration / % 3 4 6 8 10

[0066] Example 16

[0067] The difference between this embodiment and embodiment 15 is that the components of the auxiliary agent also include a penetrant, the penetrant is lactic acid, and the molar ratio between the lactic acid and the zinc chloride in the chloride salt solution is 1:1.5.

[0068] As shown in Table 4, the difference between Examples 16-20 is that the molar ratio between lactic acid and zinc chloride (hereinafter referred to as "LA:Zn") is different.

[0069] Table 4 Molar ratio between lactic acid and zinc chloride (LA:Zn)

[0070] sample Example 16 Embodiment 17 Embodiment 18 Embodiment 19 Embodiment 20 LA:Zn / % 1:1.5 1:2 1:3 1:4 1:4.5

[0071] Embodiment 21

[0072] The difference between this embodiment and embodiment 3 is that the rejuvenation agent is prepared by mixing the amino oligosaccharide aqueous solution of preparation example 1 and commercially available gibberellin in a weight ratio of 10:1.

[0073] Embodiment 22

[0074] The difference between this embodiment and embodiment 3 is that the rejuvenation agent is prepared by mixing the amino oligosaccharide aqueous solution of preparation example 1, commercially available gibberellin and commercially available high-efficiency chlorpyrifos in a weight ratio of 10:1:1.

[0075] Comparative Example

[0076] Comparative Example 1

[0077] A pine wood nematode disease nematicide, wherein the mass fractions of the components in the nematicide are as follows: 2.0% of emamectin benzoate, 2.1% of a synergist, 25.0% of a surfactant, 10.0% of a defoamer, and the remainder is supplemented to 100% by a solvent; the surfactant is Tween 60, the defoamer is a silicone defoamer, the solvent is prepared by mixing DMF and deionized water in a weight ratio of 1:4, and the synergist is prepared by mixing piperonyl butoxide (mass fraction in the nematicide is 1.0%) and potassium chloride (mass fraction in the nematicide is 1.1%).

[0078] Comparative Example 2

[0079] The difference between this comparative example and Example 3 is that Lufuda is replaced with the same weight of deionized water.

[0080] Comparative Example 3

[0081] The difference between this comparative example and Example 3 is that the rejuvenation agent is replaced with the same weight of deionized water.

[0082] Comparative Example 4

[0083] The difference between this comparative example and Example 3 is that emamectin benzoate is replaced with deionized water of the same weight.

[0084] Performance testing methods

[0085] In the following testing process, all operations involving spray application are carried out at 1500mL / hm 2 Apply the pesticide in the dosage.

[0086] 1. Field Nematode Control Test Test purpose: To evaluate the control effect of the nematicides of Examples 1-22 and Comparative Examples 1-4 on pine wood nematode disease.

[0087] Test agents: nematicides of Examples 1-22 and Comparative Examples 1-4.

[0088] Control agent: irrigation water.

[0089] Experimental setup: A mountain forest with Masson pine trees was selected and divided into three experimental areas of equal size, A, B, and C (each with an area of ​​10 mu). 26 healthy Masson pine trees with a tree age of 20 were selected as sample trees in each experimental area. Five healthy Masson pine trees with a tree age of 20 were selected as control trees under the same conditions outside the experimental area.

[0090] Pine wood nematodes were captured in a Masson pine forest area where emamectin benzoate was applied for three consecutive years in the past five years, and resistant nematodes were obtained after artificial breeding. 5,000 resistant nematodes were artificially inoculated on each sample pine tree and the control pine tree.

[0091] 30 days after inoculation, the trunks and crowns of the sample pine trees in areas A, B, and C were sprayed with pesticides (in the same area, one sample pine tree corresponds to one test agent), and the trunks and crowns of 5 control pine trees were sprayed with irrigation water.

[0092] After 30 days of application, the treatment effect was evaluated and calculated according to the following formula:

[0093]

[0094] Among them, IP is the disease susceptibility index of the test pine trees. IP is set at different levels according to the symptoms of pine disease, that is, the disease susceptibility index IP of pine trees is divided into 12 levels, with increasing degrees, level 0 means no disease, level 1 means a small amount (≦10%) of branches and leaves turn brown, level 2 means 10-20% of branches and leaves turn brown, level 3 means 20-30% of branches and leaves turn brown, level 4 means 30-40% of branches and leaves turn brown, level 5 means 40-50% of branches and leaves turn brown, level 6 means 50-60% of branches and leaves turn brown, level 7 means 60-70% of branches and leaves turn brown, level 8 means 70-80% of branches and leaves turn brown, level 9 means 80-90% of branches and leaves turn brown, level 10 means more than 90% of branches and leaves turn brown, and level 11 means the whole plant is dead.

[0095] After the IP statistics of the three areas A, B and C were completed, the average value of the corresponding treatment effects of the sample pine trees in the three areas that were treated with the same test agent was taken and recorded as the treatment rate. The calculation results of the treatment rate are shown in Table 5.

[0096] Table 5 Treatment rate

[0097] sample Treatment rate / % sample Treatment rate / % Example 1 78.9 Embodiment 14 85.4 Example 2 79.7 Embodiment 15 84.1 Example 3 80.4 Example 16 86.1 Example 4 80.9 Embodiment 17 86.7 Example 5 81.1 Embodiment 18 87.5 Example 6 82.7 Embodiment 19 88.2 Example 7 83.9 Embodiment 20 88.5 Example 8 85.6 Embodiment 21 82.5 Example 9 87.1 Embodiment 22 84.7 Example 10 87.5 Comparative Example 1 31.6 Embodiment 11 83.2 Comparative Example 2 47.5 Example 12 84.6 Comparative Example 3 65.6 Embodiment 13 85.2 Comparative Example 4 58.2

[0098] 2. Field Monochamus alternatus control experiment

[0099] The difference between this test and the aforementioned field nematode control test is that the test agent only includes the nematicides of Example 3 and Comparative Examples 1-4, and 100 pine alternatus larvae are inoculated into each sample pine tree at the same time as the inoculation of drug-resistant nematodes.

[0100] Thirty days after the spray treatment, all sample pine trees were felled and split, and the number of dead larvae of Monochamus alternatus was counted. The mortality rate of Monochamus alternatus was calculated as follows:

[0101]

[0102] D: number of living Monochamus alternatus found;

[0103] M: the number of Monochamus alternatus inoculated.

[0104] After obtaining the calculation results of the mortality rate corresponding to each test pine tree, the average of the mortality rates corresponding to the sample pine trees applied with the same test agent in the three areas was taken and recorded as the average mortality rate. The results are shown in Table 6.

[0105] Table 6 Average mortality rate

[0106] sample Average mortality rate / % Example 3 91.3 Comparative Example 1 61.7 Comparative Example 2 64.0 Comparative Example 3 71.3 Comparative Example 4 69.0

[0107] Combining Examples 1-5 and Comparative Example 1 and Table 5, it can be seen that the treatment rates measured in Examples 1-5 are much higher than those in Comparative Example 1, indicating that after applying the compound nematicide of the present application to the forest area that has been invaded by nematodes and the nematodes are resistant to emamectin benzoate, it can achieve a more ideal treatment effect than the nematicides in the related art.

[0108] Combining Example 3 with Comparative Examples 1-4 and Tables 5-6, it can be seen that when emamectin benzoate, Lufuda and rejuvenation agent are used in combination, the treatment effect achieved is relatively good. Compared with Comparative Examples 1-4, the mortality rate of pine beetles in Example 3 is also higher, indicating that the secretions of pine wood nematodes have a promoting effect on the development of its transmission medium, pine beetles. The metabolism of pine wood nematodes is blocked or dead, which reduces the nematode secretions inside the infected pine trees, resulting in the slowing down of the growth and development of pine beetle larvae, the decline of drug resistance, and the pine beetle larvae are more easily poisoned by emamectin benzoate.

[0109] Combining Example 3 and Examples 6-10 and Table 5, it can be seen that polyethylene glycol can promote the dissolution of lignin, which is conducive to the full penetration of the compound nematicide in the trunk. As the amount of polyethylene glycol increases, the treatment effect increases, but it does not show a linear change. After the mass fraction of polyethylene glycol exceeds 15%, the improvement in the treatment effect is relatively small. In order to fully save raw materials and control costs, and to achieve better performance, the mass fraction of polyethylene glycol is preferably 5-15%.

[0110] It can be seen from Example 3, Example 11-15 and Table 5 that zinc chloride can promote the swelling of cellulose and increase the distance between cellulose and hemicellulose, so the treatment effects of Example 11-15 are better than those of Example 3. In Example 11-15, the treatment effect first increases and then decreases, indicating that the amount of zinc chloride used in Example 11 is small, and the degree of cellulose swelling in the trunk is small. In Example 15, the amount of zinc chloride used is too large, and the zinc ions are complexed with hydroxyl, carbonyl and other groups in the trunk to form a large amount of flocculent insolubles, which blocks the penetration channel of the compound nematicide and affects the penetration effect of the compound nematicide. After optimization, when the chloride salt solution is a zinc chloride solution with a solute mass fraction of 4-8%, the penetration effect of the compound nematicide is relatively good.

[0111] It can be seen from Example 15, Examples 16-20 and Table 5 that after lactic acid is used as a penetrant, the lactic acid and zinc ions are complexed to form a complex chloride salt. The complex chloride salt can still promote the swelling of cellulose, and the lactic acid has occupied the complexation site, so the complex chloride salt is hindered from complexing with hydroxyl groups, carbonyl groups and other groups in the trunk, and the degree of flocculation is small, which helps to improve the penetration effect of the compound nematicide.

[0112] As the amount of lactic acid increases, the treatment effect increases, but it does not show a linear change. As the amount of lactic acid increases, the improvement in the treatment effect gradually decreases. In order to fully save raw materials and control costs, and to achieve better performance, the molar ratio between lactic acid and zinc chloride in the chloride salt solution is preferably 1: (2-4).

[0113] From Example 3, Examples 21-22 and Table 5, it can be seen that the combination of amino oligosaccharides aqueous solution and gibberellin, and the combination of amino oligosaccharides aqueous solution, gibberellin and high-efficiency chlorpyrifos can improve the effect of promoting the growth of pine trees, help the recovery of damaged parts of pine trees, and improve the treatment effect of pine trees infected with pine wood nematode disease.

[0114] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A highly effective compound nematicide for pine wood nematode disease, characterized in that: The components of the compound nematicide include a nematicide and an adjuvant, wherein the adjuvant includes a synergist, a surfactant, a solvent and a rejuvenating agent, the nematicide is a mixture of emamectin benzoate and lufudan, the synergist is piperonyl butoxide, the solvent includes DMF and a chloride solution, and the rejuvenating agent includes an amino oligosaccharide aqueous solution; in the compound nematicide, the mass fraction of lufudan is 10-20%, the mass fraction of emamectin benzoate is 0.5-3.5%, and the mass fraction of the rejuvenating agent is 2-8%; the solute in the chloride solution is potassium chloride or zinc chloride; when the solute of the chloride solution is zinc chloride, the mass fraction of the solute of the chloride solution is 4-8%.

2. The highly effective compound nematicide for pine wood nematode disease according to claim 1, characterized in that: In the compound nematicide, the mass fraction of Lufuda is 12-18%, the mass fraction of emamectin benzoate is 1.0-3.0%, and the mass fraction of the rejuvenation agent is 4-6%.

3. The highly effective compound nematicide for pine wood nematode disease according to claim 1, characterized in that: The chloride salt solution is a potassium chloride solution. The auxiliary agent also includes a penetrant, which is polyethylene glycol. The polyethylene glycol is in liquid state at a standard atmospheric pressure of 20°C.

4. The highly effective compound nematicide for pine wood nematode disease according to claim 3, characterized in that: The mass fraction of the penetrant in the compound nematicide is 5-15%.

5. The highly effective compound nematicide for pine wood nematode disease according to claim 1, characterized in that: The chloride salt solution is a zinc chloride solution with a solute mass fraction of 10%. The components of the auxiliary agent also include a penetrant, and the penetrant is lactic acid.

6. The highly effective compound nematicide for pine wood nematode disease according to claim 5, characterized in that: The molar ratio between the lactic acid and the zinc chloride in the chloride salt solution is 1:(2-4).

7. The highly effective compound nematicide for pine wood nematode disease according to claim 6, characterized in that: The amino oligosaccharide aqueous solution is prepared according to the following method: (1) adding acid-soluble chitosan to the lignite extract, stirring and dissolving, and obtaining liquid A; (2) heating solution A to 50-60°C, diluting it with water, and then keeping the temperature to react for 4-5 hours to obtain solution B; (3) Separate liquid B at 4000 rpm for 4 minutes and take the supernatant, which is the amino oligosaccharide aqueous solution.

8. A method for preparing a highly effective compound nematicide for pine wood nematode disease, characterized in that: The following steps are involved: (1) Mix emamectin benzoate and rufuda evenly to obtain a nematicide; set aside; (2) The agent and the auxiliary agent described in any one of claims 1 to 7 are mixed and stirred evenly to obtain a highly effective compound nematicide for pine wood nematode disease.

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