A composition encapsulating biological peptides and its preparation method

Abstract

The present invention relates to the field of cosmetics technology, particularly to IPC A61K8, and more specifically to a composition encapsulating a biopeptide and its preparation method. The composition comprises 30-90 parts lactose, 25-45 parts cellulose, 0.1-2 parts biopeptide liquid, 0.1-1 part blue copper peptide, 0.1-1 part vitamin C, and 1-10 parts pure water. The present invention utilizes biopeptide encapsulation technology to enhance the activity and purity of the biopeptide, optimize its storage temperature, and facilitate transportation and use.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to IPC A61K8, and more specifically to a composition encapsulating a biopeptide and a preparation method thereof. Background Art

[0002] Biopeptides are essential players in the body's diverse and complex physiological activities. All cells can synthesize peptides, and their functions are regulated by them. Compared to ordinary proteins, bioactive peptides contain more hydrophobic residues and possess diverse physiological functions, such as skin conditioning, regulating skin's ecological balance, promoting digestion and absorption, enhancing skin elasticity, and replenishing moisture.

[0003] The existing patent CN202210206256.2 discloses a skin microecological balance composition, its preparation method and application. The skin microbial balance composition includes substances such as probiotics, prebiotics, and cell-rejuvenating biological peptides. The effectiveness and long-term activity of the biological peptides are still under discussion.

[0004] As a biological product, biopeptides need to be refrigerated at low temperatures to maintain their activity. Traditional methods require them to be refrigerated and are extremely inconvenient to transport and carry. When using biopeptides, they need to be thawed and activated, which is a cumbersome operation. After thawing, the effective activity of the biopeptides is greatly reduced, and it is even more difficult to ensure the activity of the biopeptides in the products when added to them. The finished products prepared with biopeptides need to be stored at low temperatures for freshness preservation, otherwise they will become inactivated and the storage time will be short. Summary of the Invention

[0005] In order to solve the problems in the prior art, the first aspect of the present invention provides a composition encapsulating a biopeptide, wherein the raw materials for preparing the composition include lactose, cellulose, biopeptide liquid, blue copper peptide, vitamin C, and pure water.

[0006] The composition encapsulating the biological peptide comprises, by weight, 30 to 90 parts of lactose, 25 to 45 parts of cellulose, 0.1 to 2 parts of biological peptide liquid, 0.1 to 1 part of blue copper peptide, 0.1 to 1 part of vitamin C, and 1 to 10 parts of pure water; more preferably, it comprises 55 parts of lactose, 35 parts of cellulose, 1.8 parts of biological peptide liquid, 0.5 part of blue copper peptide, 1 part of vitamin C, and 6.7 parts of pure water.

[0007] Preferably, the method for preparing the bio-peptide liquid is as follows: removing impurities and moisture from non-genetically modified beans and crushing them for 20 to 40 minutes, inoculating Bacillus, stirring and mixing them thoroughly, sealing them, sun-drying them for 6 to 8 days, storing them at low temperature for 90 to 180 days to obtain fermented products, and thawing and filtering them to obtain the bio-peptide liquid.

[0008] Preferably, the non-genetically modified beans are one of non-genetically modified soybeans, non-genetically modified black beans, and non-genetically modified mung beans; more preferably, they are non-genetically modified soybeans.

[0009] Preferably, the non-genetically modified soybeans are purchased from the soybean producing area in Northeast China.

[0010] Preferably, the mass of the non-genetically modified beans is 1 kg, and the amount of added Bacillus is 1 gram.

[0011] Preferably, the effective substance content in the Bacillus is 3 to 8×10 11 CFU / g; further preferably, 5×10 11 CFU / g.

[0012] Preferably, the Bacillus includes one or more of Bacillus cereus, Bacillus licheniformis, and Bacillus coagulans; more preferably, it is Bacillus coagulans.

[0013] Preferably, the Bacillus coagulans is purchased from Xi'an Wusehua Biotechnology Co., Ltd., model number WSH-382.

[0014] In the present invention, Bacillus coagulans is selected to ferment with non-genetically modified soybeans, which can comprehensively decompose the non-genetically modified soybean protein and improve the decomposition efficiency and decomposition rate of the non-genetically modified soybeans. At the same time, Bacillus coagulans uses non-genetically modified soybeans as raw materials, which can inhibit the growth of harmful bacteria and produce some secondary products such as vitamins and amino acids during the reproduction and decomposition process, which are beneficial to improving the moisture retention of the biological peptides and at the same time improving the purity of the biological peptides.

[0015] Preferably, the lactose is 50 to 60 parts by weight.

[0016] Preferably, the lactose is 80-200 mesh; more preferably, it is 200 mesh.

[0017] Preferably, the lactose is purchased from Leprino Food, model number 35-303.

[0018] Preferably, the cellulose is in an amount of 30 to 40 parts by weight.

[0019] Preferably, the cellulose includes one or more of hydroxyethyl cellulose, ethyl cellulose, and hydroxyethyl methyl cellulose; more preferably, it is hydroxyethyl cellulose.

[0020] Preferably, the hydroxyethyl cellulose is purchased from Ashland (China) Investment Co., Ltd., and the model number is Natrosol 250 "HHR" PC and CS.

[0021] In the present invention, by adding 50 to 60 parts of lactose and 30 to 40 parts of cellulose, the stability of the biopeptide composition can be improved, forming a coating with moderate hardness and softness. When there is too much lactose and too little fiber, the resulting coating is hard, and the wall breaking and dissolution during use are slow, affecting the use effect. In addition, during the preparation process, the hard coating will cause a large amount of the composition to break during the forging process, making it unable to encapsulate the biopeptide, thereby reducing the stability of the biopeptide in the composition.

[0022] Preferably, the blue copper peptide is purchased from Guangzhou Zuoyue Fine Chemical Co., Ltd.

[0023] In the present invention, a certain amount of blue copper peptide is added to stimulate the formation of glucosamine while improving the customer's sense of use. The inventor speculates that the copper bond of a certain amount of blue copper peptide can bind to the amino acid GHK-CU (a complex composed of three amino acids and a copper ion), which can stimulate the production of glucosamine, increase skin thickness, and tighten the skin. On the other hand, in the process of using the composition, the wall of the package can be broken, thereby causing the active substance to be slowly released. After the slow release, the blue copper peptide is naturally blue, forming a short blue color on the skin surface, and gently massaged for absorption. Changing from blue to colorless can give customers a sense of satisfaction with skin absorption, thereby generating self-restraint and forming a good habit of daily use.

[0024] Preferably, the vitamin C is purchased from Suzhou Fulide Biotechnology Co., Ltd., model number 0022.

[0025] Preferably, the low temperature is -15 to -40°C; more preferably, it is -25°C.

[0026] Preferably, the specific steps of thawing and filtering to obtain the biological peptide liquid are: (1) thawing the fermentation product stored at low temperature to 18-25° C.; (2) filtering by membrane filtration to obtain small molecular peptide chains with a molecular weight of 500-1000.

[0027] A second aspect of the present invention provides a method for preparing an encapsulated biopeptide composition, comprising the following steps:

[0028] Water, lactose and cellulose are dissolved and mixed at high temperature, then cooled, ground and pulverized by a wall breaker, and transferred to a physical forging machine. Vitamin C, biological peptide liquid and blue copper peptide are continuously added to the physical forging machine, and the equipment is started. The beads are vibrated and polished into fine beads of uniform size, which are sieved to remove impurities and suctioned to remove powder and dust to prepare a composition.

[0029] Preferably, the high temperature is 75-85°C; more preferably, it is 80°C.

[0030] Preferably, the mesh size of the sieve is 30 to 80 meshes; more preferably, it is 60 to 80 meshes.

[0031] Preferably, the pressure of the physical forging press is 0.5-0.8 MPa.

[0032] In the present invention, by adding 30 to 90 parts of lactose, 25 to 45 parts of cellulose, 0.1 to 1 part of blue copper peptide, 0.1 to 1 part of vitamin C, and 1 to 10 parts of pure water, 0.1 to 2 parts of biological peptide liquid can be encapsulated therein, which can maximize the purity of the active product, have good storage stability, are not easily affected by external temperature, and are convenient to use. The inventor unexpectedly discovered that a small molecule biological peptide with a molecular weight of 500 to 1000 obtained by fermenting Bacillus coagulans and non-transgenic soybeans and then filtering through a membrane and mixing with other substances by physical forging to obtain a composition encapsulated with biological peptides. Water, lactose, and cellulose are the outer coatings. The surface of the forged particles is uneven and smooth, and the equipment needs to be started and vibrated to form fine beads of uniform size. The fine beads polished uniformly have good storage stability. Under a microscope, the small molecule polypeptides and blue copper peptides inside are connected, which are not easily affected by external temperature, ensuring the active purity of the product and convenient to use. During use, by gently kneading and touching, the wall of the package is broken and the active ingredients are slowly released. Small molecule biological peptides are generally composed of 2 to 10 amino acids, with a molecular weight of less than 1000, and have extremely strong activity and penetration ability, and are easily absorbed directly by the skin. The molecular weight of biological peptides is small enough and the permeability is very strong. They can quickly penetrate the skin and be quickly absorbed by the skin, reaching the dermis.

[0033] Beneficial effects

[0034] 1. In the present invention, Bacillus coagulans is selected to ferment with non-genetically modified soybeans, which can improve the decomposition efficiency and decomposition rate of non-genetically modified soybeans. At the same time, Bacillus coagulans uses non-genetically modified soybeans as raw materials, which is beneficial to improving the moisture retention of bio-peptides and at the same time improving the purity of bio-peptides.

[0035] 2. In the present invention, a certain amount of blue copper peptide is added to stimulate the formation of glucosamine while improving the user experience.

[0036] 3. In the present invention, by using the encapsulation technology of biological peptides, 30 to 90 parts of lactose, 25 to 45 parts of cellulose, 0.1 to 1 part of blue copper peptide, 0.1 to 1 part of vitamin C, and 1 to 10 parts of pure water are added, and 0.1 to 2 parts of biological peptide liquid can be encapsulated therein, which can ensure the active purity of the product to the greatest extent, has good storage stability, is not easily affected by external temperature, and is convenient and easy to use. DETAILED DESCRIPTION

[0037] Example 1

[0038] In a first aspect, this embodiment provides a composition encapsulating a biopeptide, wherein the raw materials for preparing the composition are, by weight, 55 parts of lactose, 35 parts of cellulose, 1.8 parts of biopeptide liquid, 0.5 parts of blue copper peptide, 1 part of vitamin C, and 6.7 parts of pure water.

[0039] The preparation method of the bio-peptide liquid comprises the following steps: removing impurities and dehumidifying non-genetically modified beans and crushing them for 30 minutes, inoculating Bacillus, fully stirring and mixing, sealing, sun-drying for 7 days, storing at low temperature for 150 days to obtain fermented products, and thawing and filtering to obtain the bio-peptide liquid.

[0040] The specific steps of thawing and filtering to obtain the biological peptide liquid are: (1) thawing the fermented product stored at low temperature to 25° C.; (2) filtering by membrane filtration to obtain small molecular peptide chains with a molecular weight of 500 to 1000.

[0041] The non-genetically modified beans are non-genetically modified soybeans.

[0042] The non-genetically modified soybeans were purchased from the soybean producing area in Northeast China.

[0043] The mass of the non-genetically modified beans is 1 kg, and the amount of added Bacillus coagulans is 1 gram.

[0044] The effective substance content in the Bacillus is 5×10 11 CFU / g.

[0045] The Bacillus coagulans was purchased from Xi'an Wusehua Biotechnology Co., Ltd., model number WSH-382.

[0046] The lactose is 200 mesh.

[0047] The lactose was purchased from Leprino Food, model number 35-303.

[0048] The cellulose is hydroxyethyl cellulose.

[0049] The hydroxyethyl cellulose was purchased from Ashland (China) Investment Co., Ltd., and the model number was Natrosol 250 "HHR" PC and CS.

[0050] The blue copper peptide was purchased from Guangzhou Zuoyue Fine Chemical Co., Ltd.

[0051] The vitamin C was purchased from Suzhou Fulide Biotechnology Co., Ltd., model number 0022.

[0052] The low temperature is -25°C.

[0053] A second aspect of this embodiment provides a method for preparing an encapsulated biopeptide composition, comprising the following steps:

[0054] Water, lactose and cellulose are dissolved and mixed at 80° C., cooled, ground and pulverized with a wall breaker, and transferred to a physical forging machine. Vitamin C, biological peptide liquid and blue copper peptide are continuously added to the 0.6 MPa physical forging machine, and the equipment is started. The beads are vibrated and polished into fine beads of uniform size, sieved with an 80-mesh filter to remove impurities, and powder and dust are removed by suction to prepare a composition.

[0055] Example 2

[0056] The specific implementation of Example 2 is the same as that of Example 1, except that the raw materials, by weight, are 50 parts of lactose, 40 parts of cellulose, 1.8 parts of biological peptide liquid, 0.1 parts of blue copper peptide, 1 part of vitamin C, and 2 parts of pure water.

[0057] Example 3

[0058] The specific implementation of Example 3 is the same as that of Example 1, except that the raw materials, by weight, are 60 parts of lactose, 30 parts of cellulose, 1.8 parts of biological peptide liquid, 2 parts of blue copper peptide, 1 part of vitamin C, and 10 parts of pure water.

[0059] Comparative Example 1

[0060] The specific implementation of Comparative Example 1 is the same as that of Example 1, except that the raw materials, by weight, are 70 parts of lactose, 20 parts of cellulose, 1.8 parts of biological peptide liquid, 0.1 parts of blue copper peptide, 1 part of vitamin C, and 2 parts of pure water.

[0061] Comparative Example 2

[0062] The specific implementation of Comparative Example 2 is the same as that of Example 1, except that the raw materials, by weight, are 80 parts of lactose, 10 parts of cellulose, 1.8 parts of biological peptide liquid, 0.1 parts of blue copper peptide, 1 part of vitamin C, and 10 parts of pure water.

[0063] Comparative Example 3

[0064] The specific implementation of Comparative Example 3 is the same as that of Example 1, except that, by weight, it contains 55 parts of lactose, 35 parts of cellulose, 1.8 parts of biological peptide liquid, 1 part of vitamin C, and 6.7 parts of pure water.

[0065] Comparative Example 4

[0066] The specific implementation of Comparative Example 4 is the same as that of Example 1, except that the preparation method of the encapsulated biopeptide composition comprises the following steps:

[0067] Water, lactose and cellulose are dissolved and mixed at 60° C., cooled, ground and pulverized with a wall breaker, and transferred to a physical forging machine. Vitamin C, biological peptide liquid and blue copper peptide are continuously added to the physical forging machine. The equipment is started, and the beads are vibrated and polished into fine beads of uniform size. The beads are sieved with an 80-mesh filter to remove impurities, and powder and dust are removed by suction to prepare a composition.

[0068] Comparative Example 5

[0069] The specific implementation of Comparative Example 5 is the same as that of Example 1, except that the preparation method of the encapsulated biopeptide composition comprises the following steps:

[0070] Water, lactose and cellulose are dissolved and mixed at 80° C., cooled and ground with a wall-breaking machine, vitamin C, biological peptide liquid and blue copper peptide are added to the wall-breaking machine, and mixed for 30 minutes to form fine beads of uniform size, which are sieved with an 80-mesh filter to remove impurities, and powder and dust are removed by suction to prepare a composition.

[0071] Performance Testing

[0072] 1. Stability test:

[0073] The composition prepared in Example 1 was placed at 4°C, 25°C, 35°C, 45°C, and 50°C, and its appearance / color, pH, peptide content, Bacillus content, and vitamin C content were observed and measured for 12 weeks. The measurement results are recorded in Table 1. The composition was then cycled from -10°C to 40°C, then back down to -10°C after 24 hours, once, twice, and three times. The degree of change in the composition's properties is recorded in Table 1. The degree of change is as follows: 0 = no change; 1 = minor change; 2 = significant change; 3 = severe change. The data in Table 1 demonstrate that the composition prepared in Example 1 performed normally and had good stability.

[0074] The appearance of the compositions was observed under a microscope, and the stability, peptide content, Bacillus content, and vitamin C content of Examples 1 to 3 and Comparative Examples 1 and 2 were measured and recorded in Table 2.

[0075] Determination Method: Visually observe the appearance and color of the composition; measure the pH of the composition using a pH meter; determine the peptide and vitamin C content in the composition using gas chromatography, and calculate the percentage of peptide and vitamin C content; determine the Bacillus content per gram of the composition using a plate colony count method, with the data rounded off. The softness of the wrapping was tested using a softness tester.

[0076] 2. Moisturizing test:

[0077] Twenty-two female volunteers aged between 23 and 55 and two male volunteers were selected. The composition prepared in Example 1 was applied to the left half of the volunteers' faces at room temperature of 25°C and humidity of 45%. The moisture content of the left and right stratum corneum was measured before application and 4, 8, and 24 hours after application. The results are reported in Table 3. Table 3 shows that the composition in Example 1 exhibits strong moisturizing properties.

[0078] Table 1

[0079]

[0080]

[0081]

[0082] Table 2

[0083]

[0084] Table 3

[0085]

Claims

1. A composition encapsulating a biological peptide, characterized in that: The raw materials for its preparation, by weight, are 55 parts of lactose, 35 parts of cellulose, 1.8 parts of biological peptide liquid, 0.5 parts of blue copper peptide, 1 part of vitamin C, and 6.7 parts of pure water; The preparation method of the biopeptide liquid comprises the following steps: removing impurities and dehumidifying non-genetically modified beans and crushing them for 30 minutes, inoculating Bacillus, fully stirring and mixing, sealing, sun-drying for 7 days, storing at low temperature for 150 days to obtain a fermented product, thawing and filtering to obtain the biopeptide liquid; The specific steps of thawing and filtering to obtain the biological peptide liquid are: (1) thawing the fermented product stored at low temperature to 25° C.; (2) filtering by membrane filtration to obtain a small molecule peptide chain with a molecular weight of 500 to 1000; The non-genetically modified beans are non-genetically modified soybeans; The bacillus is Bacillus coagulans; The mass of the non-genetically modified beans is 1 kg, and the amount of Bacillus coagulans added is 1 gram; The effective substance content in the Bacillus is 5×10 11 CFU / g; The lactose is 200 mesh; The cellulose is hydroxyethyl cellulose; The cryogenic temperature is -25°C; The preparation method of the encapsulated bio-peptide composition comprises the following steps: dissolving and mixing water, lactose, and cellulose at 80° C., cooling the mixture, grinding and pulverizing the mixture with a wall-breaking machine, transferring the mixture into a physical forging press, continuously adding vitamin C, bio-peptide liquid, and blue copper peptide into the 0.6 MPa physical forging press, starting the equipment, vibrating and grinding the mixture into fine beads of uniform size, sieving the mixture with an 80-mesh filter to remove impurities, and removing powder and dust by suction to prepare the composition.

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