Agricultural composition containing fenoxycarb and lufenuron

Abstract

The present invention belongs to the technical field of pesticide compounding and relates to an agricultural composition containing fenoxycarb and lufenuron. The present invention provides an agricultural composition containing fenoxycarb and lufenuron, wherein the active ingredients include fenoxycarb and lufenuron, and the weight ratio of fenoxycarb to lufenuron is 30:1 to 1:15. The present invention also provides a method for preparing a suspension concentrate from fenoxycarb and lufenuron, wherein the suspension concentrate comprises the following components in weight percentage: 20-60% active ingredient, 1-5% dispersant, 1-5% wetting agent, 0.1-1% thickener, 0.05-0.1% defoamer, 1-5% antifreeze, and water to 100%. Laboratory toxicity tests conducted by the present invention revealed that fenoxycarb and lufenuron exhibit excellent insecticidal efficacy within a given ratio range, exhibiting a synergistic effect.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding and relates to an agricultural composition containing fenoxycarb and lufenuron. Background Art

[0002] Fenoxycarb, chemically known as 2-(p-phenoxyphenoxy)ethylcarbamate, is a non-terpene carbamate compound with stomach and contact toxicity, as well as an insect growth regulator, boasting a broad spectrum of insecticides. However, its insecticidal effects are non-neurotropic, manifesting as strong juvenile hormone activity against a wide range of insects. This can kill eggs, inhibit adult metamorphosis, and larval molting, resulting in larval mortality in the late pupal or pupal stages. It is a highly specific insecticide and harmless to bees and beneficial organisms. Fenoxycarb is primarily used in warehouses to control stored-stock pests. It also has insect-bioregulatory effects, such as disrupting insect metamorphosis, spraying barns to prevent the reproduction of coleoptera and lepidoptera pests, and spraying indoor cracks to control cockroaches and fleas. It can be made into bait to control fire ants, termites and other ant colonies. It can be applied in water to inhibit mosquito larvae from developing into adult mosquitoes in cotton fields, orchards, vegetable gardens and ornamental plants. It can effectively control psyllids, scale insects, leaf rollers, etc., and is also effective against pests that have become resistant to currently commonly used pesticides.

[0003] Lufenuron is an insect chitin synthesis inhibitor, primarily used to control lepidopteran and coleopteran larvae, as well as citrus rust, on crops such as cotton, corn, vegetables, and fruit trees. It can also be used as a sanitary agent and to control parasites in animals such as cattle. The agent acts on insect larvae, hindering their molting process and causing their death. It is particularly effective against leaf-feeding caterpillars on fruit trees and other plants. Lufenuron has both stomach and contact effects and is highly ovicidal. It has good selectivity against predatory insects and is non-toxic to most adult beneficial insects. It is also low-toxic to bees, birds, and mammals, making it safe for users and crops and an environmentally friendly insecticide.

[0004] Insecticides containing a single active ingredient often suffer from varying degrees of deficiencies in agricultural pest control, such as a narrow insecticide spectrum, the tendency for pests to develop resistance after continuous use, and increased environmental pollution with frequent use. Combining active ingredients with different mechanisms of action can effectively overcome these shortcomings. Furthermore, the synergistic effect of combining two active ingredients can improve control efficacy, expand the insecticide spectrum, delay the development of pest resistance, and reduce environmental pollution, making it an important tool for integrated pest control. However, in most cases, the effects of insecticide combinations are additive, with combinations exhibiting synergistic effects being rare, especially those with highly pronounced synergistic effects and high co-toxicity coefficients. Currently, there are no reports on the combination of the two active ingredients, fenoxycarb and lufenuron. Summary of the Invention

[0005] The object of the present invention is to provide an agricultural composition containing fenoxycarb and lufenuron. The active ingredients of the agricultural composition are fenoxycarb and lufenuron compounded in a specific ratio. The two have different mechanisms of action. It has been verified that their compounding produces a good synergistic effect, expands the insecticidal spectrum, slows down the development of drug resistance, improves the insecticidal effect, and is beneficial to the sustainable development of the environment.

[0006] Based on the above purpose, the present invention provides an agricultural composition containing fenoxycarb and lufenuron, wherein the active ingredients include fenoxycarb and lufenuron; the ratio of fenoxycarb to lufenuron is 30:1 to 1:15 by mass.

[0007] It is readily apparent to those skilled in the art that multiple pesticides are often used in combination or in combination to improve the efficacy of agricultural compositions and prevent the development of drug resistance. However, due to the lack of clarity regarding the components and underlying mechanisms, it is difficult to predict whether the control effects of these combinations or combinations will be synergistic. The present inventors, through in-depth experimental research, have determined, based on comprehensive efficacy assessments demonstrated in field trials, that the insecticidal composition of the present invention exhibits excellent control effects against lepidopteran pests, surpassing the efficacy of a single agent.

[0008] Preferably, in the agricultural composition provided by the present invention, the ratio of fenoxycarb to lufenuron is 14:1 to 1:1 by mass.

[0009] More preferably, in the agricultural composition provided by the present invention, the ratio of fenoxycarb to lufenuron is 3:1 by mass.

[0010] Depending on the actual application needs, the composition of the present invention can be prepared into one of the following dosage forms: aqueous emulsion, microemulsion, suspension, wettable powder, water-dispersible granules, etc., using methods known to those skilled in the art. The functional composition also contains adjuvants required for the preparation of pesticide formulations. The adjuvants can be one or a mixture of solvents, emulsifiers, wetting agents, stabilizers, dispersants, thickeners, pH adjusters, defoamers, antifreeze agents, fillers, etc. The adjuvants described in the present invention can all be known substances, such as various adjuvants commonly used in pesticide formulations, and can vary depending on the specific situation.

[0011] The application method can be spraying, root irrigation, root soaking, seed soaking, pouring, swinging, broadcasting, furrow application, hole application, etc. Spraying is preferred for pest control, and the preferred formulation for pest control is a suspension concentrate.

[0012] Suspension concentrates are increasingly popular due to their advantages, including the absence of organic solvents, good environmental compatibility, low processing costs, low phytotoxicity, ease of dilution, application, and metering, and safe storage and transportation. The present invention also provides a method for preparing a suspension concentrate from fenoxycarb and lufenuron. The suspension concentrate comprises the following components by weight: 20-60% active ingredient, 1-5% dispersant, 1-5% wetting agent, 0.1-1% thickener, 0.05-0.1% defoamer, 1-5% antifreeze, and water to 100%.

[0013] Furthermore, in the agricultural composition provided by the present invention, the dispersant is a mixture of calcium dodecylbenzenesulfonate and nonylphenol polyoxyethylene ether in a weight ratio of 4:1, the wetting agent is a mixture of ammonium lauryl sulfate and ammonium myristyl polyether sulfate in a weight ratio of 2:1, the thickener is xanthan gum, the defoaming agent is silicone oil, and the antifreeze agent is ethylene glycol.

[0014] The composition of the present invention is mainly used for preventing and controlling insect pests in food crops and vegetables, and the insect pests are preferably Lepidoptera pests, such as cotton bollworm, diamondback moth, moth, and noctuid armyworm.

[0015] Compared with the prior art, the technical solution provided by the present invention has the following beneficial effects or advantages:

[0016] Compared to the prior art, the present invention's primary contribution lies in providing an agricultural composition containing fenoxycarb and lufenuron, which exhibits excellent control efficacy against plant pests. The inventors conducted field efficacy trials on this composition and found that within the specified ratio range, the composition is more effective against plant pests than either agent alone, demonstrating a synergistic effect. Another contribution of the present invention is the formulation of fenoxycarb and lufenuron into a suspension concentrate. The resulting suspension concentrate exhibits superior performance, including high suspension efficiency, good dispersibility, and low decomposition rate, enhancing insecticide control effectiveness. DETAILED DESCRIPTION

[0017] The technical solutions of the present invention are described below with reference to the following embodiments; however, the present invention is not limited to the following embodiments.

[0018] Example 1

[0019] This example provides an indoor combined toxicity test of an agricultural composition containing fenoxycarb and lufenuron against Plutella xylostella.

[0020] Test insects: Third-instar larvae of the diamondback moth with consistent size and physiological state were reared indoors for generations.

[0021] Test agents: fenoxycarb (purchased from Hubei Xinrunde Chemical Co., Ltd.) and lufenuron (purchased from Nanjing Bermuda Biotechnology Co., Ltd.).

[0022] Test method: The poisoned leaf method was adopted, referring to the "Agricultural Industry Standard of the People's Republic of China NY / T1154.2-2006". The specific method is as follows.

[0023] First, a leaf butterfly was punched using a 1 cm diameter hole punch, placed in a culture dish, and moisturized. Next, a capillary dropper was used to apply each treatment agent, starting at a low concentration, with 1 μL of solution applied to each leaf butterfly. After the solvent evaporated, the leaf butterfly was paired with another leaf butterfly coated with starch paste to create a poisoned leaf butterfly. The resulting leaf butterfly was then placed in a well of a 12-well tissue culture plate. Finally, a test insect was placed in each well of the tissue culture plate and placed in an intelligent insectary chamber maintained at a temperature of 26±1°C and a relative humidity of 70%. Two to four hours after inoculation, clean feed was added to the wells of the culture plate, and the insects continued to feed until monitored. Any insects that had not consumed a complete leaf butterfly were eliminated. Each treatment was replicated four times, with at least 12 poisoned leaf butterflies per replicate. A control treatment containing the corresponding organic solvent was also included, without the agent. Mortality was assessed 24 hours after treatment, and the total and dead insect counts were recorded.

[0024] SPSS statistical analysis software was used to perform statistical analysis and calculate the LC of each drug. 50 , and the co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method. The test results are shown in Table 1.

[0025] Measured toxicity index (ATI) = (standard agent LC 50 / Test reagent LC 50 )×100.

[0026] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture.

[0027] Co-toxicity coefficient (CTC) = [measured toxicity index (ATI) of the mixture / theoretical toxicity index (TTI) of the mixture] × 100.

[0028] According to the NY / T11547.7-2006 standard for the classification of combined effects of insecticides: a co-toxicity coefficient (CTC) ≥ 120 indicates a synergistic effect; a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect; and a CTC of 80 < 120 indicates an additive effect.

[0029] Table 1. Indoor combined toxicity assay

[0030]

[0031] As shown in Table 1 above, the fenoxycarb and lufenuron mass ratios of 40:1 and 1:30 exhibited an additive effect, while the fenoxycarb and lufenuron mass ratios of 30:1 to 1:15 exhibited a synergistic effect, with a co-toxicity coefficient (CTC) ≥ 120. At a fenoxycarb and lufenuron mass ratio of 3:1, the CTC reached 156.37.

[0032] Preparation Example 1

[0033] This formulation example provides a preparation method for a suspension concentrate of an agricultural composition containing fenoxycarb and lufenuron.

[0034] The suspension consists of the following components in percentage by weight: 20% of active ingredient, 1% of dispersant, 1% of wetting agent, 0.1% of thickener, 0.05% of defoaming agent, 1% of antifreeze agent, and water making up to 100%.

[0035] The active ingredient is a mixture of fenoxycarb and lufenuron in a weight ratio of 30:1, the dispersant is a mixture of calcium dodecylbenzenesulfonate and nonylphenol polyoxyethylene ether in a weight ratio of 4:1, the wetting agent is a mixture of ammonium lauryl sulfate and ammonium myristyl polyether sulfate in a weight ratio of 2:1, the thickener is xanthan gum, the defoaming agent is silicone oil, and the antifreeze is ethylene glycol.

[0036] The dispersant, wetting agent, defoamer and water in the above formula are subjected to high-speed shearing and mixed evenly, and then the active ingredients are added and sanded in a sand mill. The particle size of the material needs to be frequently tested during sand milling, and the material is sand milled to a particle size of 3-5 μm; the thickener is added to the antifreeze and mixed evenly, and then the mixture of the thickener and antifreeze is added to the liquid mixture after sand milling, and mixed evenly by high-speed shearing to obtain a suspension product.

[0037] Preparation Example 2

[0038] This formulation example provides a preparation method for a suspension concentrate of an agricultural composition containing fenoxycarb and lufenuron.

[0039] The suspension consists of the following components in percentage by weight: 30% of active ingredient, 2% of dispersant, 2% of wetting agent, 0.3% of thickener, 0.05% of defoaming agent, 1% of antifreeze agent, and water making up to 100%.

[0040] The active ingredient is a mixture of fenoxycarb and lufenuron in a weight ratio of 14:1, the dispersant is a mixture of calcium dodecylbenzenesulfonate and nonylphenol polyoxyethylene ether in a weight ratio of 4:1, the wetting agent is a mixture of ammonium lauryl sulfate and ammonium myristyl polyether sulfate in a weight ratio of 2:1, the thickener is xanthan gum, the defoaming agent is silicone oil, and the antifreeze is ethylene glycol.

[0041] Preparation Example 3

[0042] This formulation example provides a preparation method for a suspension concentrate of an agricultural composition containing fenoxycarb and lufenuron.

[0043] The suspension concentrate is composed of the following components in percentage by weight: 40% of active ingredient, 5% of dispersant, 5% of wetting agent, 1% of thickener, 0.1% of defoaming agent, 5% of antifreeze agent, and water making up to 100%.

[0044] The active ingredient is a mixture of fenoxycarb and lufenuron in a weight ratio of 3:1, the dispersant is a mixture of calcium dodecylbenzenesulfonate and nonylphenol polyoxyethylene ether in a weight ratio of 4:1, the wetting agent is a mixture of ammonium lauryl sulfate and ammonium myristyl polyether sulfate in a weight ratio of 2:1, the thickener is xanthan gum, the defoaming agent is silicone oil, and the antifreeze agent is ethylene glycol.

[0045] Preparation Example 4

[0046] This formulation example provides a preparation method for a suspension concentrate of an agricultural composition containing fenoxycarb and lufenuron.

[0047] The suspension concentrate is composed of the following components in percentage by weight: 50% of active ingredient, 5% of dispersant, 5% of wetting agent, 1% of thickener, 0.1% of defoaming agent, 5% of antifreeze agent, and water making up to 100%.

[0048] The active ingredient is a mixture of fenoxycarb and lufenuron in a weight ratio of 1:1, the dispersant is a mixture of calcium dodecylbenzenesulfonate and nonylphenol polyoxyethylene ether in a weight ratio of 4:1, the wetting agent is a mixture of ammonium lauryl sulfate and ammonium myristyl polyether sulfate in a weight ratio of 2:1, the thickener is xanthan gum, the defoaming agent is silicone oil, and the antifreeze agent is ethylene glycol.

[0049] Preparation Example 5

[0050] This formulation example provides a preparation method for a suspension concentrate of an agricultural composition containing fenoxycarb and lufenuron.

[0051] The suspension concentrate is composed of the following components in percentage by weight: 60% of active ingredient, 5% of dispersant, 5% of wetting agent, 1% of thickener, 0.1% of defoaming agent, 5% of antifreeze agent, and water making up to 100%.

[0052] The active ingredient is a mixture of fenoxycarb and lufenuron in a weight ratio of 1:15, the dispersant is a mixture of calcium dodecylbenzenesulfonate and nonylphenol polyoxyethylene ether in a weight ratio of 4:1, the wetting agent is a mixture of ammonium lauryl sulfate and ammonium myristyl polyether sulfate in a weight ratio of 2:1, the thickener is xanthan gum, the defoaming agent is silicone oil, and the antifreeze is ethylene glycol.

[0053] Preparation Example 6

[0054] This formulation example provides a preparation method for a suspension concentrate of an agricultural composition containing fenoxycarb and lufenuron.

[0055] This formulation example is intended to be compared with formulation example 3. The preparation method is the same as formulation example 3, except that the dispersant is calcium dodecylbenzenesulfonate, the wetting agent is ammonium lauryl sulfate, the thickener is xanthan gum, the defoaming agent is silicone oil, and the antifreeze agent is ethylene glycol.

[0056] The suspension concentrates prepared in Formulation Examples 1 to 6 were subjected to performance testing, including suspension rate, residue after pouring, residue after washing, and persistent foaming (foaming amount after 1 minute / mL) under different storage conditions. The suspension rate was tested according to the GB / T14825-2006 method for determining suspension rate of pesticides, the pouring property was tested according to the GB / T31737-2015 method for determining pouring property of pesticides, the persistent foaming property was tested according to the HG / T2467.5-2003 product standard for pesticide suspension concentrates, and the hot storage stability was tested according to the GB / T19136-2003 method for determining hot storage stability of coatings.

[0057] Table 1: Suspension Concentrate Performance Test

[0058]

[0059]

[0060] As shown in Table 1, the comparative Example 6 exhibits inferior suspension rate, residue after pouring, residue after washing, foaming properties, and sustained foaming properties compared to Examples 1-5, indicating that the selection of adjuvants significantly impacts the performance of the suspending agent. The present invention improves the performance of the suspending agent by selecting the adjuvants and their contents. The suspending agent of Example 6 exhibits slightly worse quality after hot storage, while the suspending agents of Examples 1-5, which utilize adjuvants in specific ratios, exhibit good quality both before and after hot storage, indicating that the selection of preferred adjuvants improves the performance of the suspending agent.

[0061] In addition, as can be seen from Formulation Examples 1 to 5, when the preferred adjuvant is selected, the performance of the resulting suspension concentrate is similar when the content of the active pharmaceutical ingredient varies within the range of 20% to 60%. This indicates that when the preferred adjuvant composition and content are used, the drug content has little effect on the performance of the dosage form. As can be seen from Formulation Example 6, when the conventional adjuvant is used, the performance of the resulting suspension concentrate is relatively poor, and replacing the adjuvant does not significantly improve the performance. This indicates that the composition and content of the preferred adjuvant significantly improve the performance of the suspension concentrate.

[0062] Example 2

[0063] This example provides an agricultural composition containing fenoxycarb and lufenuron for use in a control test of cotton bollworm.

[0064] The experimental site was Yancheng City, Jiangsu Province. The cotton variety was Simian No. 3. The experimental groups were shown in Table 2. Each treatment had three replicates and the area of ​​each treatment plot was 666.7 m 2 Each treatment was separated by a cotton film as a protective row, and each experimental plot was randomly arranged in blocks. The application time was when the second generation of cotton bollworms were at their peak, and the number of larvae was 667m. 2 According to the prescribed dosage, add 60kg of water and the dosage of active ingredient is unified to 3g / hm 2 , use Gongnong-16 type manual sprayer to spray evenly by hand.

[0065] The survey used a five-point sampling method, with samples taken at five diagonal points in each plot. Twenty cotton plants with cotton bollworm larvae were marked in the forward direction at each point, for a total of 1,200 plants marked per plot. The number of cotton bollworm larvae was counted before application, and the number of surviving bollworms was measured 3, 7, and 14 days after application. The damage and impact on other organisms were also investigated. The control efficacy of each replicate was calculated using the following formula.

[0066] Control effect = [1-(number of insects in the blank control group before application × number of insects in the treated group after application) / (number of insects in the blank control group after application × number of insects in the treated group before application)] × 100%

[0067] Table 2: Control effect of cotton bollworm

[0068]

[0069]

[0070] As shown in the test results in Table 2, the combination of fenoxycarb and lufenuron effectively controls cotton bollworms. The control efficacy against cotton bollworms was significantly superior to that of the two control agents 3, 7, and 14 days after application, demonstrating a synergistic effect. Formulation Examples 1-5, using the preferred adjuvant, exhibited significantly better control efficacy than Formulation Example 6. Observations throughout the field trials showed that cotton grew well within the tested dosage range, and no phytotoxicity was observed with any of the treatment agents, demonstrating their safety for cotton.

[0071] As described above, the present invention can be better implemented. The above embodiments only describe the preferred implementation methods of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various changes and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope determined by the present invention.

Claims

1. An agricultural composition containing fenoxycarb and lufenuron, characterized in that: The active ingredients include fenoxycarb and lufenuron; The mass ratio of fenoxycarb and lufenuron is 30:1 to 1:15; Prepare a suspension concentrate, which is composed of the following components in weight percentage: 20-60% of the active ingredient, 1-5% of a dispersant, 1-5% of a wetting agent, 0.1-1% of a thickener, 0.05-0.1% of a defoaming agent, 1-5% of an antifreeze agent, and water to make up to 100%; The dispersant is a mixture of calcium dodecylbenzenesulfonate and nonylphenol polyoxyethylene ether in a weight ratio of 4:1, the wetting agent is a mixture of ammonium lauryl sulfate and ammonium myristyl polyether sulfate in a weight ratio of 2:1, the thickener is xanthan gum, the defoaming agent is silicone oil, and the antifreeze is ethylene glycol.

2. The agricultural composition according to claim 1, characterized in that Calculated by mass, the ratio of fenoxycarb to lufenuron is 14:1 to 1:

1.

3. The agricultural composition according to claim 1, characterized in that In terms of mass ratio, the ratio of fenoxycarb and lufenuron is 3:

1.

4. Use of the agricultural composition according to any one of claims 1 to 3 in preventing and controlling insect pests on grain crops and vegetables.

5. The use according to claim 4, characterized in that The insect pests are lepidoptera pests.

6. The use according to claim 4, characterized in that The insect pests are cotton bollworms, diamondback moths, stem borers or noctuids.

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