A bactericide composition for preventing and controlling bacterial wilt of Momordica grosvenori

Abstract

The present invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and controlling bacterial wilt of Momordica grosvenori. A bactericide composition for preventing and controlling bacterial wilt of Momordica grosvenori, wherein the pesticide composition is prepared by compounding aureomycin with phenazine methosulfate, allyl isothiazole or zinc thiazole. After the two active ingredients are compounded, the bactericide composition of the present invention has a synergistic effect on the target pathogen of bacterial wilt of Momordica grosvenori, and can improve the control effect thereof.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and controlling bacterial wilt of Siraitia grosvenorii. Background Art

[0002] Bacterial wilt of Siraitia grosvenorii is a bacterial disease caused by Ralstonia solanacearum, which mainly damages the base and stem of the plant. At the initial stage of the disease, the epidermis at the base of the plant is rough and easy to crack, the vascular bundle of the stem turns brown, and the diseased leaves show chlorosis. At the beginning of the disease, the top leaves of the plant wilt and droop, and then the whole plant's leaves wither. The disease develops rapidly, and the diseased plants die within a few days. Bacterial wilt of Siraitia grosvenorii can be controlled by spraying chemical agents. However, long-term use of a single chemical agent is likely to cause problems such as drug resistance, pesticide residues, and environmental pollution. At the same time, it will also reduce the control effect on the disease. The compound formulation can delay the generation and development of disease resistance and improve the control effect on the disease. Therefore, it is of great significance to screen compound formulations with synergistic effects on bacterial wilt of Siraitia grosvenorii.

[0003] Aureofusarin is a nucleoside antibiotic containing adenine and a special undecose sugar skeleton, which was isolated from the fermentation broth of Streptomyces aureofaciens SP-371 by the Shanghai Institute of Pesticide Research. It has good control effects on bacterial diseases such as citrus canker and bacterial blight of rice. The structural formula of aureofusarin is as follows:

[0004]

[0005] The inventors screened compound formulations of aureofusarin and phenazine methosulfate, allyl isothiazole, or thiazole zinc through a large number of indoor experiments. The relevant compound formulations have synergistic effects on the target within a certain range. There is no relevant report in this regard yet.

[0006] The information disclosed in this background art section is only intended to enhance the overall understanding of the present invention and should not be regarded as an admission or any form of implication that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0007] The purpose of the present invention is to provide a bactericide composition for preventing and controlling bacterial wilt of Siraitia grosvenorii to solve the problems existing in the long-term use of a single chemical agent.

[0008] To achieve the above purpose, the present invention provides the following technical solutions:

[0009] A bactericide composition for preventing and controlling bacterial wilt of Siraitia grosvenorii, wherein the pesticide composition is compounded from aureofusarin and phenazine methosulfate, allyl isothiazole, or thiazole zinc.

[0010] More specifically, the mass ratio of aureomycin and phenazine-1-carboxylic acid is 1-20:20-1.

[0011] More specifically, the mass ratio of aureomycin and propamocarb hydrochloride is 1-8:30-1.

[0012] More specifically, the mass ratio of aureomycin and zinc thiazole is 1-5:9-1.

[0013] More specifically, the mass ratio of aureomycin and zinc thiazole is 5:1.

[0014] The present invention also provides the application of the pesticide composition in preventing and treating bacterial wilt of Momordica grosvenori.

[0015] More specifically, the bacterial wilt of Momordica grosvenori is caused by the infection of Ralstonia solanacearum.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) After the two active ingredients of the fungicide composition of the present invention are compounded, it has a synergistic effect on the target pathogen of bacterial wilt of Momordica grosvenori, and can improve the control effect on it.

[0018] (2) The action mechanisms of the two active ingredients of the fungicide composition of the present invention are different, which can delay the generation and development of pathogen drug resistance.

[0019] (3) The fungicide composition of the present invention can reduce the application dosage of pesticides, reduce the control cost of diseases, reduce pesticide residues, and reduce environmental pollution. Detailed implementation manners

[0020] The technical solutions of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the scope of protection of the present invention.

[0021] Example : Indoor bioactivity test after compounding with aureomycin

[0022] 1. Tested pathogens: Ralstonia solanacearum, obtained by separating and purifying samples of bacterial wilt of Momordica grosvenori collected from Momordica grosvenori plantations in Guangxi in the laboratory.

[0023] The tested pathogens were cultured in NA medium in a bacterial incubator at 30°C for 48h. Single colonies were picked and inoculated into 30 mL of NB medium. At 30°C and 120 r / min, the culture was shaken until the tested pathogens reached the logarithmic growth phase, and then the concentration of the bacterial suspension was adjusted to 1×107 cfu / mL, for standby.

[0024] 2. Culture Medium

[0025] NA Medium: 6 g of polypeptone, 1 g of yeast extract, 3 g of beef extract, 15 g of sucrose, 17 g of agar, made up to 1 L with distilled water and adjusted to pH 7.0 with 10 mol / L NaOH.

[0026] NB Medium: 6 g of polypeptone, 1 g of yeast extract, 3 g of beef extract, 15 g of sucrose, made up to 1 L with distilled water and adjusted to pH 7.0 with 10 mol / L NaOH.

[0027] 3. Test Agents

[0028] 94% aureomycin technical, 95% phenazine methosulfate technical, 95% probenazole technical, 95% thiazole zinc technical. All of the above agents are commercially available.

[0029] Dissolve the test agents with dimethyl sulfoxide and dilute them with 0.1% (mass fraction) Tween-80 aqueous solution to prepare single-agent stock solutions. Set multiple groups of ratios, and set 5 series of mass concentration gradients for each single agent and each group of mixed agents. Each mass concentration of the liquid medicine is 50 mL, for standby.

[0030] 4. Test Method (Refer to "NT / T 1156.16 - 2008 Guidelines for Pesticide Bioassay in the Laboratory - Part 16: Turbidity Method for Testing the Inhibitory Effect on Bacterial Growth of Fungicides")

[0031] Under aseptic conditions, quantitatively pipette the liquid medicine from low concentration to high concentration into 30 mL of NB medium in sequence, and set the treatment with sterile water as the blank control. Each treatment is set with 4 replicates. Then, inoculate 100 μL of the test pathogen suspension into the NB medium after each treatment, and place it in an incubator at 30 °C with shaking at 120 r / min.

[0032] Measure the turbidity of each treatment before starting the culture. When the test pathogen in the blank control treatment reaches the logarithmic growth phase, measure the turbidity of each treatment again, and calculate the growth inhibition rate of the test pathogen.

[0033]

[0034] 4. Data Analysis: Perform linear regression analysis with the logarithm of the agent concentration as x and the probit value of the corresponding growth inhibition rate as y, calculate the EC 50 , and calculate the co-toxicity coefficient (CTC value) of the mixed agent according to Sun Yunpei method.

[0035] The synergistic effect of the mixed use of agents was evaluated according to the co-toxicity coefficient (CTC), that is, CTC ≤ 80 was antagonistic effect, 80 < CTC < 120 was additive effect, and CTC ≥ 120 was synergistic effect. The experimental results are shown in Table 1-3.

[0036] Table 1 Indoor bioactivity determination of the compound of aureomycin and phenazine methosulfate against Ralstonia solanacearum

[0037] Names and Ratios of Medicaments <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Aureofungin 6.4135 100.0000 -- -- Shenqinmycin 20.4526 31.3579 -- -- Aureofungin 1:Shenqinmycin 20 14.7677 43.4292 34.6265 125.4218 Aureofungin 1:Shenqinmycin 15 12.6082 50.8677 35.6480 142.6944 Aureofungin 1:Shenqinmycin 10 10.0170 64.0262 37.5981 170.2911 Aureofungin 1:Shenqinmycin 5 7.4664 85.8982 42.7982 200.7049 Aureofungin 1:Shenqinmycin 1 5.9961 106.9612 65.6789 162.8546 Aureofungin 5:Shenqinmycin 1 3.8876 164.9732 88.5596 186.2849 Aureofungin 10:Shenqinmycin 1 4.2025 152.6115 93.7598 162.7686 Aureofungin 15:Shenqinmycin 1 5.2281 122.6736 95.7099 128.1724 Aureofungin 20:Shenqinmycin 1 5.4374 117.9516 96.7313 121.9373

[0038] As can be seen from Table 1, in the mass ratio range of 1-20:20-1 of aureomycin and phenazine methosulfate, the co-toxicity coefficients were all greater than 120, that is, the bioactivity of the compound of aureomycin and phenazine methosulfate against Ralstonia solanacearum showed a synergistic effect.

[0039] Table 2 Indoor bioactivity determination of the compound of aureomycin and propylisothiazolinone against Ralstonia solanacearum

[0040] Names and Ratios of Medicaments <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Aureofungin 6.4135 100.0000 -- -- Probenazole 11.0854 57.8554 -- -- Aureofungin 1:Probenazole 30 6.0272 106.4093 59.2149 179.7002 Aureofungin 1:Probenazole 20 8.3137 77.1438 59.8623 128.8687 Aureofungin 1:Probenazole 10 4.9281 130.1414 61.6867 210.9716 Aureofungin 1:Probenazole 5 7.1248 90.0166 64.8795 138.7443 Aureofungin 1:Probenazole 1 6.6322 96.7025 78.9277 122.5203 Aureofungin 5:Probenazole 1 4.3695 146.7788 92.9759 157.8676 Aureofungin 8:Probenazole 1 5.5248 116.0857 95.3173 121.7887

[0041] As can be seen from Table 2, in the mass ratio range of 1-8:30-1 of aureomycin and propylisothiazolinone, the co-toxicity coefficients were all greater than 120, that is, the bioactivity of the compound of aureomycin and propylisothiazolinone against Ralstonia solanacearum showed a synergistic effect.

[0042] Table 3 Indoor bioactivity determination of the compound of aureomycin and phenazine methosulfate against Ralstonia solanacearum

[0043] Names and Ratios of Medicaments <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Aureofungin 6.4135 100.0000 -- -- Shenqinmycin 2.7347 234.5230 -- -- Aureofungin 1:Shenqinmycin 9 1.0547 608.0876 221.0707 275.0648 Aureofungin 1:Shenqinmycin 5 0.8023 799.3893 212.1025 376.8882 Aureofungin 1:Shenqinmycin 3 2.1448 299.0256 200.8922 148.8487 Aureofungin 1:Shenqinmycin 1 3.1124 206.0628 167.2615 123.1980 Aureofungin 3:Shenqinmycin 1 1.8781 341.4887 133.6307 255.5465 Aureofungin 5:Shenqinmycin 1 1.2156 527.5995 122.4205 430.9732

[0044] As can be seen from Table 3, in the mass ratio range of 1-5:9-1 of aureomycin and phenazine methosulfate, the co-toxicity coefficients were all greater than 120, that is, the bioactivity of the compound of aureomycin and phenazine methosulfate against Ralstonia solanacearum showed a synergistic effect. Especially when the mass ratio was 5:1, the co-toxicity coefficient reached 430.9732, and the synergistic effect was the most significant.

[0045] In summary, it can be seen that when aureomycin of the present invention is compounded with phenazine methosulfate, propylisothiazolinone or thiazole zinc in a certain mass ratio range, their co-toxicity coefficients are all higher than 120, showing a synergistic effect, which can improve the control effect on bacterial wilt of Siraitia grosvenorii and is not easy to produce drug resistance.

[0046] The foregoing description of specific exemplary embodiments of the invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that, according to the above teachings, many modifications and variations are possible. The purpose of selecting and describing the exemplary embodiments is to explain the particular principles of the invention and its practical application so that those skilled in the art can implement and utilize the various different exemplary embodiments of the invention, as well as various different selections and modifications. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. Application of a bactericide composition in preventing and controlling bacterial wilt of Momordica grosvenori, characterized in that, The fungicide composition consists of aureofusarin and phenazine-1-carboxylic acid; The mass ratio of aureofusarin to phenazine-1-carboxylic acid is 1-20:20-1.

2. The application according to claim 1, wherein The bacterial wilt of Momordica grosvenori is caused by the infection of Ralstonia solanacearum.

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