A multifunctional automatic analyzer and analysis method thereof

Abstract

The present application discloses a multifunctional automatic analyzer and its analysis method, comprising a reaction disk module, a sample disk module located on the left, and at least two reagent disk modules located on the right; a sample needle module is located between the sample disk module and the reaction disk module, and an R1 reagent needle module and an R2 reagent needle module are located between the reaction disk module and the R1 reagent disk module and the R2 reagent disk module, respectively; a front cleaning module and a rear cleaning module are located at the front and rear of the reaction disk module, respectively; an R1 stirring module is located between the R1 reagent needle module and the rear cleaning module, and an R2 stirring module is located between the R2 reagent needle module and the front cleaning module; and a transmission optical unit module and a scattering optical unit module are located along the circumferential direction of the reaction disk module. The above-mentioned multifunctional automatic analyzer has a reasonable overall layout design and can realize multiple project tests such as transmission and scattering.

Description

Technical Field

[0001] The present application relates to the field of biochemical detection technology, and in particular to a multifunctional automatic analyzer and an analysis method thereof. Background Art

[0002] Automatic analyzers are currently the most widely used type of analytical products. They can replace manual labor to complete the measurement of various indicators, greatly improving the level of automation in clinical laboratories, increasing the efficiency of medical testing, and improving the quality and reliability of test results.

[0003] Automatic analyzers are characterized by their ability to mimic manual operations, completing a series of analytical processes, including sample loading, reagent addition, mixing, photometry, and cleaning. These instruments typically include components such as a sample tray, reagent tray, reaction tray, loading mechanism, stirring mechanism, and cleaning mechanism. Each structure requires a fixed motion and layout to achieve the desired process. Currently, the overall layout design of commercially available automatic analyzers is irrational, resulting in complex motion structures and high failure rates. Furthermore, the optical detection method for absorbance measurement in reaction cups is limited, limiting the number of tests that can be performed. Summary of the Invention

[0004] The purpose of this application is to provide a multifunctional automatic analyzer with a reasonable overall layout design, which can realize multiple test items such as transmission, scattering, etc. Another purpose of this application is to provide an analysis method for the multifunctional automatic analyzer.

[0005] To achieve the above-mentioned object, the present application provides a multifunctional automatic analyzer, comprising a reaction tray module, a sample tray module located on the left side of the reaction tray module, and at least two reagent tray modules located on the right side of the reaction tray module, wherein the two reagent tray modules are an R1 reagent tray module and an R2 reagent tray module;

[0006] There is a sample needle module between the sample disk module and the reaction disk module, there is an R1 reagent needle module between the R1 reagent disk module and the reaction disk module, and there is an R2 reagent needle module between the R2 reagent disk module and the reaction disk module;

[0007] A front cleaning module is arranged at the front position of the reaction disk module, a rear cleaning module is arranged at the rear position of the reaction disk module, an R1 stirring module is arranged between the R1 reagent needle module and the rear cleaning module, and an R2 stirring module is arranged between the R2 reagent needle module and the front cleaning module;

[0008] A transmission optical unit module and a scattering optical unit module are arranged along the circumference of the reaction disk module.

[0009] In some embodiments, the multifunctional automatic analyzer further includes an ISE module, and the ISE module is disposed between the sample disk module and the reaction disk module.

[0010] In some embodiments, the multifunctional automatic analyzer further includes a glycated hemoglobin cleaning pool module, and the glycated hemoglobin cleaning pool module is arranged between the reaction disk module and the sample needle module.

[0011] In some embodiments, the reaction disk module includes a thermostatic bath filled with a heat preservation medium, the thermostatic bath is provided with a thermostatic bath liquid level sensor, a cuvette rack for loading cuvettes is installed in the thermostatic bath, and the cuvette rack is driven to rotate by a reaction disk drive motor.

[0012] In some embodiments, the sample tray module includes a sample tray cover and a sample tray barcoder located outside the sample tray cover, a quality control tray and a sample rack are installed in the sample tray cover, the sample rack is connected to the quality control tray, and the quality control tray is driven to rotate by a sample tray drive motor; the quality control tray is provided with a quality control tray refrigeration bin, a first Peltier and a first water-cooled radiator are installed at the bottom of the quality control tray refrigeration bin, and the quality control tray refrigeration bin is also provided with quality control tray bottom insulation cotton and quality control tray side wall insulation cotton that wrap the quality control tray refrigeration bin.

[0013] In some embodiments, the reagent tray module includes a reagent tray pot body and a reagent tray barcode reader located on the outside of the reagent tray pot body, a central bracket and a reagent tray rack are installed in the reagent tray pot body, the reagent tray rack is connected to the central bracket, and the central bracket is driven to rotate by a reagent tray drive motor; a second Peltier and a second water-cooled radiator are installed at the bottom of the reagent tray pot body, and the reagent tray pot body is also provided with thermal insulation cotton on the outside of the reagent bin and thermal insulation cotton on the bottom of the reagent bin that wraps the reagent tray rack.

[0014] The present application also provides an analysis method using a multifunctional automatic analyzer, comprising:

[0015] S05: The reaction cup is transported to the cleaning position by the rotation of the reaction disk module, and the cleaning module cleans the reaction cup;

[0016] S06: The reaction disk module rotates to transport the reaction cup to the sample dispensing position, and the sample needle module dispenses the sample into the reaction cup;

[0017] S07: After the sample needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the sample needle;

[0018] S09: The reaction disk module rotates to transport the reaction cup to the first reagent dispensing position, and the R1 reagent needle module dispenses reagent 1 into the reaction cup;

[0019] S10: After the R1 reagent needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle;

[0020] S11: The reaction disk module rotates to transport the reaction cup to the first stirring position, and the R1 stirring module mixes the sample and reagent 1 in the reaction cup;

[0021] S12: After the R1 stirring module completes the stirring work, it performs the stirring rod cleaning work;

[0022] S13: If the transmission optical test project is performed alone, after a certain period, the transmission optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0023] S14: If the scattered optical detection project is carried out alone, then after a certain period, the scattered optical unit module will detect the colorimetric cup multiple times, and the software will calculate the absorbance through the algorithm, and obtain the detection result after comparing it with the standard reaction curve. S15: If the transmission and scattered optical detection projects are carried out simultaneously, then after a certain period, the transmission optical unit module and the scattered optical unit module will detect the colorimetric cup multiple times at the same time, and the software will calculate the absorbance through the algorithm, and obtain the detection result after comparing it with the standard reaction curve.

[0024] In some embodiments, after S07, the analysis method further includes S08;

[0025] S08: Furthermore, if the test for the glycated hemoglobin project is to be performed, the sample needle module needs to be cleaned at the glycated hemoglobin cleaning pool module position after completing the injection work.

[0026] In some embodiments, after S15, the analysis method further includes S16 and S17;

[0027] S16: Furthermore, if the test item is an ISE module test item, after a certain period, the sample needle module injects the reaction solution in the reaction cup into the ISE module for testing to obtain the test result;

[0028] S17: After the sample needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the sample needle.

[0029] In some embodiments, the analysis method further comprises:

[0030] S18: Furthermore, if the test is for a dual-reagent project, after S09, after a certain period, the reaction disk module rotates to transport the reaction cup to the second reagent dispensing position, and the R2 reagent needle module dispenses reagent 2 into the reaction cup;

[0031] S19: After the R2 reagent needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle;

[0032] S20: The reaction disk module rotates to transport the reaction cup to the second stirring position, and the R2 stirring module mixes the sample, reagent 1 and reagent 2 in the reaction cup;

[0033] S21: After the R2 stirring module completes the stirring work, it performs the stirring rod cleaning work;

[0034] S22: If the transmission optical test project is performed alone, after a certain period, the transmission optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0035] S23: If the scattered optical test project is performed alone, after a certain period, the scattered optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0036] S24: If the transmission and scattered optical tests are performed simultaneously, after a certain period, the transmission optical unit module and the scattered optical unit module simultaneously perform multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0037] S25: Furthermore, if the project is tested by the ISE module, then after a certain period, the sample needle module directly adds the sample to the ISE module to obtain the test result;

[0038] S26: After the sample needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the sample needle.

[0039] In some embodiments, the analysis method further comprises:

[0040] S27: Furthermore, if the test is for a three-reagent project, then after S20, after a certain period, the reaction disk module rotates to transport the reaction cup to the first reagent dispensing position, and the R1 reagent needle module dispenses reagent 3 into the reaction cup;

[0041] S28: After the R1 reagent needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle;

[0042] S29: The reaction disk module rotates to transport the reaction cup to the first stirring position, and the R1 stirring module mixes the sample, reagent 1, reagent 2, and reagent 3 in the reaction cup;

[0043] S30: After the R1 stirring module completes the stirring work, it performs the stirring rod cleaning work;

[0044] S31: If the transmission optical test project is performed alone, after a certain period, the transmission optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0045] S32: If the scattered optical test project is performed alone, after a certain period, the scattered optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0046] S33: If the transmission and scattered optical tests are performed simultaneously, after a certain period, the transmission optical unit module and the scattered optical unit module simultaneously perform multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0047] S34: Further, if the project is tested by the ISE module, then after a certain period, the sample needle module directly adds the sample to the ISE module to obtain the test result;

[0048] S35: After the sample needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the sample needle.

[0049] In some embodiments, the analysis method further comprises:

[0050] S36: Furthermore, if the test is for a four-reagent project, then after S29, after a certain period, the reaction disk module rotates to transport the reaction cup to the second reagent dispensing position, and the R2 reagent needle module dispenses reagent 4 into the reaction cup;

[0051] S37: After the R2 reagent needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle;

[0052] S38: The reaction disk module rotates to transport the reaction cup to the second stirring position, and the R2 stirring module mixes the sample, reagent 1, reagent 2, reagent 3, and reagent 4 in the reaction cup;

[0053] S39: After the R2 stirring module completes the stirring work, it performs the stirring rod cleaning work;

[0054] S40: If the transmission optical test project is performed alone, after a certain period, the transmission optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0055] S41: If the scattered optical test project is performed alone, after a certain period, the scattered optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0056] S42: If the transmission and scattered optical tests are performed simultaneously, after a certain period, the transmission optical unit module and the scattered optical unit module simultaneously perform multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0057] S43: Further, if the project is tested by the ISE module, then after a certain period, the sample needle module directly adds the sample to the ISE module to obtain the test result;

[0058] S44: After the sample needle module completes the dispensing work, it performs the cleaning work of the inner and outer walls of the sample needle.

[0059] Compared with the above background technology, the multifunctional automatic analyzer provided by the present application includes a sample disk module, a reaction disk module, a sample needle module, a front cleaning module, a rear cleaning module, a reagent disk module, a reagent needle module, a stirring module, a transmission optical unit module, and a scattering optical unit module. The sample disk module is located on the left side of the reaction disk module, and the reagent disk module is located on the right side of the reaction disk module and there are at least two of them, the two reagent disk modules are the R1 reagent disk module and the R2 reagent disk module; the sample needle module is located between the sample disk module and the reaction disk module, the R1 reagent needle module is located between the R1 reagent disk module and the reaction disk module, the R2 reagent needle module is located between the R2 reagent disk module and the reaction disk module, the front cleaning module is located at the front position of the reaction disk module, the rear cleaning module is located at the rear position of the reaction disk module, the R1 stirring module is located between the R1 reagent needle module and the rear cleaning module, and the R2 stirring module is located between the R2 reagent needle module and the front cleaning module; the transmission optical unit module and the scattering optical unit module are arranged along the circumferential direction of the reaction disk module. The multifunctional automatic analyzer has a reasonable overall layout design and can realize multiple project tests such as transmission and scattering. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without any creative work.

[0061] Figure 1 A schematic diagram of the structure of a multifunctional automatic analyzer provided in an embodiment of the present application;

[0062] Figure 2 A schematic structural diagram of a reaction disk module provided in an embodiment of the present application;

[0063] Figure 3A schematic structural diagram of a sample tray module provided in an embodiment of the present application;

[0064] Figure 4 A schematic cross-sectional view of a sample tray module provided in an embodiment of the present application;

[0065] Figure 5 A schematic diagram of the structure of the reagent disc module provided in an embodiment of the present application;

[0066] Figure 6 A schematic cross-sectional view of a reagent disc module provided in an embodiment of the present application;

[0067] Figure 7 This is a schematic diagram of the structure of the glycated hemoglobin cleaning pool module provided in an embodiment of the present application.

[0068] in:

[0069] 01-sample tray module, 02-reaction tray module, 03-sample needle module, 04-front cleaning module, 05-post cleaning module, 06-R1 reagent tray module, 07-R2 reagent tray module, 08-R1 reagent needle module, 09-R2 reagent needle module, 10-R1 stirring module, 11-R2 stirring module, 12-transmission optical unit module, 13-ISE module, 14-scattering optical unit module, 15-glycosylated hemoglobin cleaning pool module, 16-constant temperature bath, 17-Drive bracket, 18-Reaction disk driven wheel, 19-Reaction disk driving wheel, 20-Reaction disk driving motor, 21-Constant temperature bath support, 22-Cuvette holder, 23-Cuvette, 24-Constant temperature bath liquid level sensor, 25-Cuvette mounting plate locking cover, 26-Cuvette mounting plate, 27-Sample tray cover, 28-Sample tray driven wheel, 29-Sample tray synchronous belt, 30-Sample tray driving motor, 31-Sample tray driving wheel, 32-Sample tray barcode reader bracket, 33-Sample Rack, 34-quality control tray, 35-sample tray barcode reader, 36-sample tray cover, 37-sample tray cover insulation cotton, 38-sample tray upper shaft, 39-quality control bin outer retaining ring, 40-sample tube, 41-tube clamp, 42-first Peltier, 43-first water cooling radiator, 44-quality control tray refrigeration bin, 45-quality control tray bottom insulation cotton, 46-quality control tray side wall insulation cotton, 47-reagent tray pot, 48-reagent bin plastic retaining ring, 49-reagent tray rack, 50-reagent bottle, 51 -Center bracket, 52-sample cover reed switch, 53-reagent tray driven wheel, 54-reagent tray synchronous belt, 55-reagent tray driving wheel, 56-reagent tray drive motor, 57-reagent tray barcode reader bracket, 58-reagent tray barcode reader, 59-reagent bin top insulation cotton, 60-sealing strip, 61-reagent bin outer insulation cotton, 62-reagent bin bottom insulation cotton, 63-second Peltier, 64-second water-cooled radiator, 65-glycated hemoglobin cleaning pool, 66-cleaning pool bracket. DETAILED DESCRIPTION

[0070] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0071] In order to enable those skilled in the art to better understand the present application, the present application is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0072] Please refer to Figures 1 to 7 ,in, Figure 1 This is a schematic diagram of the structure of the multifunctional automatic analyzer provided in the embodiment of the present application. Figure 2 This is a schematic diagram of the structure of the reaction disk module provided in the embodiment of the present application. Figure 3 This is a schematic diagram of the structure of the sample tray module provided in an embodiment of the present application. Figure 4 This is a cross-sectional schematic diagram of a sample tray module provided in an embodiment of the present application. Figure 5 This is a schematic diagram of the structure of the reagent disc module provided in the embodiment of the present application. Figure 6 This is a cross-sectional schematic diagram of a reagent disc module provided in an embodiment of the present application. Figure 7 This is a schematic diagram of the structure of the glycated hemoglobin cleaning pool module provided in an embodiment of the present application.

[0073] like Figure 1 As shown, in a first specific embodiment, the present application provides a multifunctional automatic analyzer, comprising a sample tray module 01, a reaction tray module 02, a sample needle module 03, a pre-cleaning module 04, a post-cleaning module 05, a reagent tray module, a reagent needle module, a stirring module, a transmission optical unit module 12, an ISE module 13, a scattering optical unit module 14, a glycated hemoglobin cleaning pool module 15, and a user operating system. The sample tray module 01 is used to hold a specimen container containing a specimen, the reagent tray module is used to hold a reagent container containing a reagent, the sample needle module 03 is used for sample dispensing, the reagent needle module is used for reagent tray dispensing, the stirring module is used for stirring and mixing, and the optical unit is used for optical detection.

[0074] The reaction tray module 02 is placed in the center of the mounting surface. The outer ring of the reaction tray is fixed to the surface. Eight cuvette holders are evenly spaced along the inner ring of the reaction tray. Each cuvette holder can hold 20 cuvettes. The drive mechanism of the reaction tray module 02 rotates the inner ring of the reaction tray, rotating and positioning the cuvettes. The reaction tray module 02 features a thermostat to maintain the reaction solution in the cuvettes at 37°C.

[0075] The sample tray module 01 is located to the left of the reaction tray module 02. There are 95 sample positions distributed along the inner, middle, and outer circumferences of the sample tray. They are used to hold sample tubes containing test samples, calibration solutions, or quality control solutions. The sample tray module 01 can be rotated and positioned under the drive of its drive mechanism.

[0076] There are at least two reagent disc modules, each of which is used to perform at least two different assay units for different types of analysis. Figure 1 For example, two identical reagent tray modules are configured and located on the right side of the reaction tray module 02. The two reagent tray modules are R1 reagent tray module 06 and R2 reagent tray module 07. The reagent tray module located at the front right side of the reaction tray module 02 is named R2 reagent tray module 07, and the reagent tray module located at the rear right side of the reaction tray module 02 is named R1 reagent tray module 06; 5 groups of reagent bottle holders are distributed on the inner circle of the R2 reagent tray module 07, and each group of reagent bottle holders can hold 9 kinds of reagents, for a total of 45 reagent bottles; the reagent tray module can drive the inner circle of the reagent tray to rotate in the driving mechanism to realize the rotation and positioning of the reagent bottles.

[0077] The sample needle module 03 is positioned between the sample tray module 01 and the reaction tray module 02. Its drive mechanism drives the sample needle 03 to draw a sample from any of the three sample tubes in the inner, middle, or outer circles of the sample tray and transfer it to the corresponding reaction cup in the reaction tray module 02. The sample needle module 03 is also equipped with a sample cleaning pool (glycosylated hemoglobin cleaning pool module 15) for cleaning the inner and outer walls of the sample needle.

[0078] The glycated hemoglobin cleaning pool module 15 includes a glycated hemoglobin cleaning pool 65 and a cleaning pool support 66 . The glycated hemoglobin cleaning pool 65 is disposed at the upper end of the cleaning pool support 66 . The interior of the glycated hemoglobin cleaning pool 65 forms a cavity for cleaning the sample needle.

[0079] An R2 reagent needle module 09 is positioned between the reaction disk module 02 and the R2 reagent disk module 07, and an R1 reagent needle module 08 is positioned between the reaction disk module 02 and the R1 reagent disk module 06. Each module's drive mechanism drives the reagent needle to draw reagent from the corresponding reagent bottle and add it to the corresponding reaction cup in the reaction disk module 02. Reagent needle cleaning pools (front cleaning module 04 and rear cleaning module 05) are positioned along the movement paths of the reagent needles of the R1 reagent needle module 08 and the R2 reagent needle module 09, respectively, for cleaning the inner and outer walls of the reagent needles.

[0080] A front cleaning module 04 is located in front of the reaction disk module 02, and a rear cleaning module 05 is located in the rear. Each cleaning module includes five cleaning probes distributed along the circumference of the reaction disk, enabling acid-base cleaning, deionized water cleaning, and wiping cleaning. A glycated hemoglobin cleaning pool module 15 is located between the reaction disk module 02 and the sample probe module 03. This pool includes a cleaning pool, a cleaning valve, and associated tubing.

[0081] The R1 stirring module 10 and the R2 stirring module 11 are distributed along the circumference of the reaction disk. The R1 stirring module 10 is located between the R1 reagent needle module 08 and the post-cleaning 05, and the R2 stirring module 11 is located between the R2 reagent needle module 09 and the pre-cleaning 04. The driving mechanisms of each module can drive the stirring rod to mix the sample and reagent solution in the reaction cup evenly.

[0082] The transmission optical unit module 12 is distributed along the circumference of the reaction disk module 02 and is placed between the post-cleaning module 05 and the sample needle module 03. It is responsible for measuring the absorbance of the reaction liquid in the reaction cup. The device includes a light source, an optical measurement channel, a grating spectrometer, and a photoelectric detection element.

[0083] The ISE module 13 is placed between the sample disk module 01 and the reaction disk module 02. Specifically, the injection port of the ISE module 13 is distributed on the sample needle running track of the sample disk module 01. It uses an electrochemical method, that is, an electrode method to measure electrolyte concentration.

[0084] The scattering optical unit module 14 is responsible for measuring the absorbance of the reaction solution in the reaction cup. The device includes a light source, a scattering aperture, a rear lens, and a photoelectric detection element.

[0085] In summary, this embodiment provides a multifunctional automatic analyzer, which relates to the technical field of medical automated detection equipment; the multifunctional automatic analyzer has a reasonable overall layout design and includes transmission, scattering, ISE and glycation functions and components; it can realize transmission, scattering, ISE and glycation project tests; it includes transmission and scattering function modules, which can realize simultaneous transmission and scattering tests and output test results; it includes an ISE function module, which can realize simultaneous biochemical and ISE project tests and support high-speed ISE detection; the glycation function is integrated into the high-speed analytical instrument to realize high-throughput glycation project detection.

[0086] Furthermore, the multifunctional automatic analyzer's various motion units have been optimized for low failure rates. Furthermore, the timing for adding samples, reagents, stirring, and cleaning has been optimized, resulting in a reasonable operating sequence, fast overall operation, and high detection efficiency.

[0087] Specifically, in some embodiments, Figure 2As shown, the reaction disk module 02 comprises a thermostatic bath 16, a drive bracket 17, a reaction disk driven pulley 18, a reaction disk driving pulley 19, a reaction disk drive motor 20, a thermostatic bath support 21, a cuvette holder 22, cuvettes 23, a thermostatic bath liquid level sensor 24, a cuvette mounting tray locking cover 25, and a cuvette mounting tray 26. The thermostatic bath 16 is filled with 37°C constant-temperature water, the liquid level of which is controlled by the thermostatic bath liquid level sensor 24. The reaction disk drive motor 20 rotates the reaction disk driving pulley 19, which in turn rotates the reaction disk driven pulley 18. The reaction disk driven pulley 18 then rotates the cuvette holder 22 and the cuvettes 23 mounted thereon within the thermostatic bath 16, stopping at designated locations as needed.

[0088] In some embodiments, as Figure 3 As shown, the sample tray module 03 is composed of a sample tray housing 27, a sample tray driven wheel 28, a sample tray synchronous belt 29, a sample tray driving motor 30, a sample tray driving wheel 31, a sample tray barcode reader bracket 32, a sample rack 33, a quality control tray 34, and a sample tray barcode reader 35. The sample tray barcode reader 35 is located outside the sample tray housing 27, the quality control tray 34 is installed in the sample tray housing 27, and the sample rack 33 is connected to the quality control tray 34. The sample tray driving motor 3 drives the sample tray driving wheel 31 to rotate, and the sample tray driving wheel 31 drives the sample tray driven wheel 28 to rotate through the sample tray synchronous belt 29. The sample tray driven wheel 28 then drives the quality control tray 34 and the sample rack 33 to rotate in the sample tray housing 27 and stop at a specified position as required. In addition, the sample information can be read by the sample tray barcode reader 35 installed on the outside of the sample tray module 03.

[0089] In some embodiments, as Figure 4 As shown, the sample tray module 03 also includes a sample tray cover 36, sample tray cover insulation 37, sample tray upper shaft 38, quality control compartment outer retaining ring 39, sample tubes 40, tube clamps 41, a first Peltier 42, a first water-cooled radiator 43, a quality control tray refrigeration compartment 44, quality control tray bottom insulation 45, and quality control tray sidewall insulation 46. The sample tray module 03 is divided into three circles: inner, middle, and outer. The quality control tray refrigeration compartment 44 provides cooling. The first Peltier 42 and first water-cooled radiator 43 are installed at the bottom of the quality control tray refrigeration compartment 44. There can be one or more first Peltier 42 and first water-cooled radiator 43. Furthermore, to maintain the temperature of the quality control tray refrigeration compartment 44 within the range of 8-15°C, the sample tray cover insulation 37, the quality control tray bottom insulation 45, and the quality control tray sidewall insulation 46 are installed.

[0090] In some embodiments, as Figure 5As shown, the reagent tray module 06 is composed of a reagent tray pot 47, a reagent compartment plastic retaining ring 48, a reagent tray rack 49, a reagent bottle 50, a center bracket 51, a sample cover reed switch 52, a reagent tray driven pulley 53, a reagent tray timing belt 54, a reagent tray driving pulley 55, a reagent tray drive motor 56, a reagent tray barcode reader bracket 57, and a reagent tray barcode reader 58. The sample cover reed switch 52 uses a reed switch, also known as a reed switch, which is an electrical switch operated by an applied magnetic field and has the characteristics of rapid response, small size, light weight, wear resistance, and long life. The reagent tray barcode reader 58 is located on the outside of the reagent tray pot body 47, the central bracket 51 is installed in the reagent tray pot body 47, the reagent tray rack 49 is connected to the central bracket 51, and the reagent tray drive motor 56 drives the reagent tray active wheel 55 to rotate. The reagent tray active wheel 55 drives the reagent tray driven wheel 53 to rotate through the reagent tray synchronous belt 54. The reagent tray driven wheel 53 then drives the central bracket 51 and the reagent tray rack 49 and the reagent bottle 50 placed thereon to rotate, and stops at a designated position as needed. In addition, the reagent information can be read by the reagent tray barcode reader 58 installed on the outside of the reagent tray module 06.

[0091] In some embodiments, as Figure 6 As shown, the reagent tray module 06 also includes a reagent compartment top insulation cotton 59, a sealing strip 60, a reagent compartment outside insulation cotton 61, a reagent compartment bottom insulation cotton 62, a reagent compartment upper cover insulation cotton (not shown), a second Peltier 63, and a second water-cooled radiator 64. The reagent tray module 06 has a refrigeration function, and the second Peltier 63 and the second water-cooled radiator 64 are installed at the bottom of the reagent tray pot 47. The second Peltier 63 and the second water-cooled radiator 64 can be one or more. At the same time, in order to make the reagent tray pot 47 internal temperature can be maintained within the range of 2-8 ° C, the reagent compartment top insulation cotton 59, the sealing strip 60, the reagent compartment outside insulation cotton 61 and the reagent compartment bottom insulation cotton 62 are installed.

[0092] In summary, the present application provides a multifunctional automatic analyzer with a reasonable overall structural design, optimized design of each motion unit structure, and low failure rate. At the same time, the timing of adding samples, adding reagents, stirring and cleaning has been optimized, the operating timing is reasonable, the overall operating speed is fast, and the detection efficiency is high. The multifunctional automatic analyzer is equipped with a scattering function module, which has the advantages of high sensitivity, high precision and fast detection. The multifunctional automatic analyzer integrates the glycation function into a high-speed analytical instrument to realize high-throughput glycation project detection, and is also equipped with a glycated hemoglobin cleaning pool module. For the detection of glycated hemoglobin projects, the depth of contamination of the sample needle is deep, resulting in insufficient cleaning of the sample needle, causing cross contamination and too much liquid hanging on the needle wall, resulting in effective control of the use of liquid throwing.

[0093] The present application also provides an analysis method for a multifunctional automatic analyzer, which uses the multifunctional automatic analyzer and designs a reasonable working sequence based on the optimization of the overall structure of the multifunctional automatic analyzer.

[0094] The analysis method specifically includes the following process:

[0095] Multiple cuvettes are placed on a reaction tray device that is used to carry the cuvettes and drive them to rotate or stop at a predetermined position. For a specific embodiment, the initial operation process is as follows:

[0096] S01: The instrument is turned on, the system initialization begins and resets;

[0097] S02: After the instrument starts working, water is poured into the thermostatic bath of the reaction disk module 02. When the water reaches a predetermined level, the flow stops and the halogen lamp is turned on.

[0098] S03: The water in the reaction disk module 02 starts to circulate, and the heating rod heats the water to a preset temperature;

[0099] S04: After the temperature stabilizes, the sample needle module 03, the reagent needle module, and the stirring module also perform cleaning work respectively.

[0100] Based on the above initial operation process, the analysis method includes the following steps:

[0101] S05: The reaction cup is transported to the cleaning position by the reaction disk module 02, and the cleaning module cleans the reaction cup;

[0102] S06: The reaction disk module 02 rotates to transport the reaction cup to the sample dispensing position, and the sample needle module 03 dispenses the sample into the reaction cup;

[0103] S07: After the sample needle module 03 completes the dispensing work, it performs the cleaning work on the inner and outer walls of the sample needle;

[0104] S09: The reaction disk module 02 rotates to transport the reaction cup to the first reagent dispensing position, and the R1 reagent needle module 08 dispenses reagent 1 into the reaction cup;

[0105] S10: After the R1 reagent needle module 08 completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle;

[0106] S11: The reaction disk module 02 rotates to transport the reaction cup to the first stirring position, and the R1 stirring module 10 mixes the sample and reagent 1 in the reaction cup;

[0107] S12: After the stirring module 10 completes the stirring operation, it performs a stirring rod cleaning operation;

[0108] S13: If the transmission optical test is performed alone, after a certain period, the transmission optical unit module 12 performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0109] S14: If the scattered optical test is performed alone, after a certain period, the scattered optical unit module 14 performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0110] S15: If the transmission and scattering optical detection projects are carried out simultaneously, after a certain period, the transmission optical unit module 12 and the scattering optical unit module 14 simultaneously detect the colorimetric cup multiple times, and the software calculates the absorbance through the algorithm, and obtains the detection result by comparing it with the standard reaction curve.

[0111] It should be noted that the above steps include not only separate transmission detection and separate scattering detection, but also simultaneous transmission and scattering detection. Therefore, this method includes three cases: separate transmission, separate scattering, and simultaneous transmission and scattering.

[0112] After step S07 , the process further includes step S08 : further, if the test is for glycated hemoglobin, the sample needle module 03 needs to perform a cleaning operation at the glycated hemoglobin cleaning pool module 15 after completing the injection operation.

[0113] In some embodiments, after step S15, the following steps are further included:

[0114] S16: Furthermore, if the test item is the one tested by the ISE module 13, then after a certain period, the sample needle module 03 injects the reaction solution in the reaction cup into the ISE module 13 for testing to obtain the test result;

[0115] S17: After the sample needle module 03 completes the dispensing operation, it performs the cleaning operation on the inner and outer walls of the sample needle.

[0116] In some embodiments, based on the above basic reagent item test, the method can also perform a dual reagent item test, further comprising the following steps:

[0117] S18: Furthermore, if it is a dual-reagent test, after S09, after a certain period, the reaction disk module 02 rotates to transport the reaction cup to the second reagent dispensing position, and the R2 reagent needle module 09 dispenses reagent 2 into the reaction cup;

[0118] S19: After the R2 reagent needle module 09 completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle;

[0119] S20: The reaction disk module 02 rotates to transport the reaction cup to the second stirring position, and the R2 stirring module 11 mixes the sample, reagent 1 and reagent 2 in the reaction cup;

[0120] S21: After the stirring module 11 completes the stirring operation, it performs a stirring rod cleaning operation;

[0121] S22: If the transmission optical test is performed alone, after a certain period, the transmission optical unit module 12 performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0122] S23: If the scattered optical test is performed alone, after a certain period, the scattered optical unit module 14 performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0123] S24: If the transmission and scattering optical tests are performed simultaneously, after a certain period, the transmission optical unit module 12 and the scattering optical unit module 14 simultaneously perform multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0124] S25: Further, if it is a test item of the ISE module 13, then after a certain period, the sample needle module 03 directly adds the sample to the ISE module 13 to obtain the test result;

[0125] S26: After the sample needle module 03 completes the dispensing operation, it performs the cleaning operation on the inner and outer walls of the sample needle.

[0126] In some embodiments, based on the above two-reagent test, the method can also perform a three-reagent test, further comprising the following steps:

[0127] S27: Furthermore, if the test is for three reagents, then after S20, after a certain period, the reaction disk module 02 rotates to transport the reaction cup to the first reagent dispensing position, and the R1 reagent needle module 08 dispenses reagent 3 into the reaction cup;

[0128] S28: After the R1 reagent needle module 08 completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle;

[0129] S29: The reaction disk module 02 rotates to transport the reaction cup to the first stirring position, and the R1 stirring module 10 mixes the sample, reagent 1, reagent 2, and reagent 3 in the reaction cup;

[0130] S30: After the stirring module 10 completes the stirring operation, it performs a stirring rod cleaning operation;

[0131] S31: If the transmission optical test is performed alone, after a certain period, the transmission optical unit module 12 performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result.

[0132] S32: If the scattered optical test is performed alone, the scattered optical unit module 14 performs multiple tests on the colorimetric cup after a certain period. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0133] S33: If the transmission and scattering optical tests are performed simultaneously, after a certain period, the transmission optical unit module 12 and the scattering optical unit module 14 simultaneously perform multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0134] S34: Further, if it is a test item of the ISE module 13, then after a certain period, the sample needle module 03 directly adds the sample to the ISE module 13 to obtain the test result;

[0135] S35: After the sample needle module 03 completes the dispensing operation, it performs the cleaning operation on the inner and outer walls of the sample needle.

[0136] In some embodiments, based on the above three-reagent test, the method can also perform a four-reagent test, further comprising the following steps:

[0137] S36: Furthermore, if the test is for a four-reagent project, then after S29, after a certain period, the reaction disk module 02 rotates to transport the reaction cup to the second reagent dispensing position, and the R2 reagent needle module 09 dispenses reagent 4 into the reaction cup;

[0138] S37: After the R2 reagent needle module 09 completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle;

[0139] S38: The reaction disk module 02 rotates to transport the reaction cup to the second stirring position, and the R2 stirring module 11 mixes the sample, reagent 1, reagent 2, reagent 3, and reagent 4 in the reaction cup;

[0140] S39: After the stirring module 11 completes the stirring operation, it performs a stirring rod cleaning operation;

[0141] S40: If the transmission optical test is performed alone, after a certain period, the transmission optical unit module 12 performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0142] S41: If the scattered optical test is performed alone, the scattered optical unit module 14 performs multiple tests on the colorimetric cup after a certain period. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0143] S42: If the transmission and scattered optical tests are performed simultaneously, after a certain period, the transmission optical unit module 12 and the scattered optical unit module 14 simultaneously perform multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result.

[0144] S43: Further, if it is a test item of the ISE module 13, then after a certain period, the sample needle module 03 directly adds the sample to the ISE module 13 to obtain the test result;

[0145] S44: After the sample needle module 03 completes the dispensing operation, it performs the cleaning operation on the inner and outer walls of the sample needle.

[0146] It should be noted that many of the components mentioned in this application are universal standard parts or components known to those skilled in the art, and their structures and principles can be known to those skilled in the art through technical manuals or conventional experimental methods.

[0147] It should be noted that, in this specification, relational terms such as first and second are merely used to distinguish one entity from other entities, but do not necessarily require or imply any actual relationship or order between these entities.

[0148] The multifunctional automatic analyzer and analysis method thereof provided by the present application are introduced in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method and core idea of ​​the present application. It should be pointed out that, for those skilled in the art, without departing from the principles of the present application, several improvements and modifications may be made to the present application, and these improvements and modifications also fall within the scope of protection of the claims of the present application.

Claims

1. A multifunctional automatic analyzer, characterized in that: It includes a reaction tray module, a sample tray module located on the left side of the reaction tray module, and at least two reagent tray modules located on the right side of the reaction tray module, wherein the two reagent tray modules are an R1 reagent tray module and an R2 reagent tray module; There is a sample needle module between the sample disk module and the reaction disk module, there is an R1 reagent needle module between the R1 reagent disk module and the reaction disk module, and there is an R2 reagent needle module between the R2 reagent disk module and the reaction disk module; A front cleaning module is arranged at the front position of the reaction disk module, a rear cleaning module is arranged at the rear position of the reaction disk module, an R1 stirring module is arranged between the R1 reagent needle module and the rear cleaning module, and an R2 stirring module is arranged between the R2 reagent needle module and the front cleaning module; A transmission optical unit module and a scattering optical unit module are arranged along the circumferential direction of the reaction disk module; The sample tray module includes a sample tray cover and a sample tray barcode reader located outside the sample tray cover, a quality control tray and a sample rack are installed in the sample tray cover, the sample rack is connected to the quality control tray, and the quality control tray is driven to rotate by a sample tray drive motor; the quality control tray is provided with a quality control tray refrigeration bin, a first Peltier and a first water-cooled radiator are installed at the bottom of the quality control tray refrigeration bin, and the quality control tray refrigeration bin is further provided with a quality control tray bottom insulation cotton and a quality control tray side wall insulation cotton wrapped around the quality control tray refrigeration bin; The reagent tray module includes a reagent tray pot body and a reagent tray barcode reader located on the outside of the reagent tray pot body. A central bracket and a reagent tray rack are installed in the reagent tray pot body. The reagent tray rack is connected to the central bracket, and the central bracket is driven to rotate by a reagent tray drive motor; a second Peltier and a second water-cooled radiator are installed at the bottom of the reagent tray pot body. The reagent tray pot body is also provided with thermal insulation cotton on the outside of the reagent bin and thermal insulation cotton on the bottom of the reagent bin that wraps the reagent tray rack.

2. The multifunctional automatic analyzer according to claim 1, characterized in that The system further comprises an ISE module, which is arranged between the sample disk module and the reaction disk module.

3. The multifunctional automatic analyzer according to claim 1, characterized in that It also includes a glycated hemoglobin cleaning pool module, which is arranged between the reaction disk module and the sample needle module.

4. The multifunctional automatic analyzer according to claim 1, characterized in that The reaction disk module includes a thermostatic bath filled with a heat preservation medium, the thermostatic bath is provided with a thermostatic bath liquid level sensor, a cuvette rack for loading cuvettes is installed in the thermostatic bath, and the cuvette rack is driven to rotate by a reaction disk drive motor.

5. An analysis method using the multifunctional automatic analyzer according to any one of claims 1 to 4, characterized in that: include: S05: The reaction cup is transported to the cleaning position by the rotation of the reaction disk module, and the cleaning module cleans the reaction cup; S06: The reaction disk module rotates to transport the reaction cup to the sample dispensing position, and the sample needle module dispenses the sample into the reaction cup; S07: After the sample needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the sample needle; S09: The reaction disk module rotates to transport the reaction cup to the first reagent dispensing position, and the R1 reagent needle module dispenses reagent 1 into the reaction cup; S10: After the R1 reagent needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle; S11: The reaction disk module rotates to transport the reaction cup to the first stirring position, and the R1 stirring module mixes the sample and reagent 1 in the reaction cup; S12: After the R1 stirring module completes the stirring work, it performs the stirring rod cleaning work; S13: If the transmission optical test project is performed alone, after a certain period, the transmission optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result. S14: If the scattered optical test project is performed alone, after a certain period, the scattered optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result. S15: If the transmission and scattering optical detection projects are carried out simultaneously, after a certain period, the transmission optical unit module and the scattering optical unit module will simultaneously detect the colorimetric cup multiple times, and the software will calculate the absorbance through the algorithm, and obtain the detection result after comparing it with the standard reaction curve.

6. The analysis method according to claim 5, characterized in that After S07, the method further includes S08; S08: If the test is for glycated hemoglobin, the sample needle module needs to be cleaned at the glycated hemoglobin cleaning pool module after completing the injection work.

7. The analysis method according to claim 5, characterized in that After S15, S16 and S17 are also included; S16: If the test item is an ISE module test item, after a certain period, the sample needle module injects the reaction solution in the reaction cup into the ISE module for testing to obtain the test result; S17: After the sample needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the sample needle.

8. The analysis method according to claim 5, characterized in that Also includes: S18: If it is a dual-reagent test, after S09, after a certain period, the reaction disk module rotates to transport the reaction cup to the second reagent dispensing position, and the R2 reagent needle module dispenses reagent 2 into the reaction cup; S19: After the R2 reagent needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle; S20: The reaction disk module rotates to transport the reaction cup to the second stirring position, and the R2 stirring module mixes the sample, reagent 1 and reagent 2 in the reaction cup; S21: After the R2 stirring module completes the stirring work, it performs the stirring rod cleaning work; S22: If the transmission optical test project is performed alone, after a certain period, the transmission optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result. S23: If the scattered optical test project is performed alone, after a certain period, the scattered optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result. S24: If the transmission and scattered optical tests are performed simultaneously, after a certain period, the transmission optical unit module and the scattered optical unit module simultaneously perform multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result. S25: If it is an ISE module test item, then after a certain period, the sample needle module directly adds the sample to the ISE module to obtain the test result; S26: After the sample needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the sample needle.

9. The analysis method according to claim 8, characterized in that Also includes: S27: If it is a three-reagent test, then after S20, after a certain period, the reaction disk module rotates to transport the reaction cup to the first reagent dispensing position, and the R1 reagent needle module dispenses reagent 3 into the reaction cup; S28: After the R1 reagent needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle; S29: The reaction disk module rotates to transport the reaction cup to the first stirring position, and the R1 stirring module mixes the sample, reagent 1, reagent 2, and reagent 3 in the reaction cup; S30: After the R1 stirring module completes the stirring work, it performs the stirring rod cleaning work; S31: If the transmission optical test project is performed alone, after a certain period, the transmission optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result. S32: If the scattered optical test project is performed alone, after a certain period, the scattered optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result. S33: If the transmission and scattered optical tests are performed simultaneously, after a certain period, the transmission optical unit module and the scattered optical unit module simultaneously perform multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result. S34: If the test item is an ISE module, then after a certain period, the sample needle module directly adds the sample to the ISE module to obtain the test result; S35: After the sample needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the sample needle.

10. The analysis method according to claim 9, characterized in that Also includes: S36: If it is a four-reagent test, then after S29, after a certain period, the reaction disk module rotates to transport the reaction cup to the second reagent dispensing position, and the R2 reagent needle module dispenses reagent 4 into the reaction cup; S37: After the R2 reagent needle module completes the dispensing work, it performs the cleaning work on the inner and outer walls of the reagent needle; S38: The reaction disk module rotates to transport the reaction cup to the second stirring position, and the R2 stirring module mixes the sample, reagent 1, reagent 2, reagent 3, and reagent 4 in the reaction cup; S39: After the R2 stirring module completes the stirring work, it performs the stirring rod cleaning work; S40: If the transmission optical test project is performed alone, after a certain period, the transmission optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result. S41: If the scattered optical test project is performed alone, after a certain period, the scattered optical unit module performs multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with the standard reaction curve to obtain the test result. S42: If the transmission and scattered optical tests are performed simultaneously, after a certain period, the transmission optical unit module and the scattered optical unit module simultaneously perform multiple tests on the colorimetric cup. The software calculates the absorbance through an algorithm and compares it with a standard reaction curve to obtain the test result. S43: If the test item is an ISE module, then after a certain period, the sample needle module directly adds the sample to the ISE module to obtain the test result; S44: After the sample needle module completes the dispensing work, it performs the cleaning work of the inner and outer walls of the sample needle.

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