A thermostatic light-proof mixing device for polypeptide solid-phase synthesis

Abstract

The application discloses a constant-temperature light-proof mixing device for polypeptide solid-phase synthesis, which comprises a cabinet body, an oscillation tank, a control console, an air bath generator, an oscillation assembly and a light-proof assembly. The control console is fixedly installed above the back side of the top plate of the cabinet body. The oscillation tank is slidably arranged in the cabinet body through slide rails at both sides. A limiting plate is arranged on the back of the oscillation tank in the cabinet body. The two sides of the oscillation tank are connected with the inside of the cabinet body through two groups of electric push rods at both ends of the limiting plate. The oscillation assembly is arranged on the bottom of the oscillation tank. A window is formed in the front side of the control console on the top plate of the cabinet body. The inside of the window is sealingly connected through two groups of heat insulation glasses from top to bottom. The light-proof assembly is fixedly installed between the two groups of heat insulation glasses. A sampling assembly is arranged below the top plate of the cabinet body. The air bath generator is fixedly installed above the back of the cabinet body. The application has the advantages that the reaction power is realized through the oscillation assembly, the reaction efficiency is improved, and the sampling and mixing can be completed under the constant-temperature light-proof condition, so that the reaction condition is stable.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of reagent mixing and sample adding equipment, in particular to a constant-temperature light-proof mixing device for polypeptide solid-phase synthesis. BACKGROUND

[0002] Polypeptide drugs are a kind of drugs with good efficacy and safety in the field of new drug development. Due to wide indications, high safety and significant efficacy, polypeptide drugs have been widely used in the prevention, diagnosis and treatment of diseases such as tumors, hepatitis, diabetes and AIDS, and have broad development prospects.

[0003] The synthesis method of polypeptides is divided into solid-phase synthesis and liquid-phase synthesis. The solid-phase synthesis method is to use resin as an insoluble solid carrier. First, an amino acid with an amino group protected by a blocking group (X) is covalently connected to the solid carrier. Under the action of a deprotection reagent, the protection group of the amino group is removed, and the carboxyl group of the second amino acid with an amino group blocked is activated by an activating reagent. The activated carboxyl group reacts with the amino group of the first amino acid connected to the solid carrier to form a peptide bond, thereby forming a dipeptide. By repeating the above operations, a polypeptide chain can be obtained. Finally, the peptide chain connected according to the sequence requirements is cut off from the resin, and after refining and purification, the polypeptide with the required sequence can be obtained.

[0004] The advantages of solid-phase synthesis mainly lie in that the initial reactants and products are connected to the solid carrier, so all reactions can be carried out in one reaction container, which is convenient for automation operation. Excessive reactants can be added to obtain high yield of products, and the products can be easily separated. The solid-phase synthesis method of polypeptides has become the mainstream method for preparing polypeptide drugs. With the development of solid-phase synthesis technology, the development of polypeptide synthesis devices has also attracted much attention.

[0005] In the laboratory, polypeptide solid-phase synthesis usually needs to be carried out under stable temperature conditions, and an air blowing device is also needed. Nitrogen is usually used as the medium to make the amino acid and the peptide chain in the reactor fully contact through blowing, so as to improve the synthesis efficiency. However, such a device occupies a large area, and the three-way valve of the reactor needs to be manually opened and closed to control the air inlet size, which is prone to operation errors and causes resin overflow. In addition, it is troublesome to add samples in some light-proof reaction conditions. SUMMARY

[0006] The purpose of the present application is to provide a constant-temperature light-proof mixing device for polypeptide solid-phase synthesis. The reaction power is realized by the oscillation assembly to improve the reaction efficiency, and the sample adding and mixing can be completed under constant-temperature light-proof conditions, so as to stabilize the reaction conditions.

[0007] The above technical purpose of the present application is realized by the following technical scheme:

[0008] A constant temperature and light-proof mixing device for solid phase synthesis of polypeptides, characterized in that it includes a cabinet, an oscillation tank, a control console, an air bath generator, an oscillation component and a light-proof component, the control console is fixedly installed on the rear side above the cabinet top plate, the front side of the cabinet is provided with an opening, the two sides of the oscillation tank are slidably arranged inside the cabinet through slide rails, a limit plate is provided inside the cabinet at the back of the oscillation tank, gaps are provided between the two ends of the limit plate and the side walls inside the cabinet, the two sides of the oscillation tank are connected to the inside of the cabinet at the two ends of the limit plate through two groups of electric push rods, the two ends of the electric push rods are hinged to the oscillation tank and the inner wall of the cabinet, the oscillation tank An oscillation component is provided at the inner bottom, a placement plate is fixedly installed above the oscillation component, a plurality of fixing openings are provided on the placement plate, a reaction tube is inserted into each of the fixing openings, a window is provided on the front side of the console on the top plate of the cabinet, the upper and lower parts of the window are sealed and connected by two groups of heat-insulating glass, the light-shielding component is fixedly installed between the two groups of heat-insulating glass, a sampling component is provided below the top plate of the cabinet, the gas bath generator is fixedly installed above the back of the cabinet, the gas bath generator is communicated with the interior of the cabinet, and the console is electrically connected and controlled to the gas bath generator, sampling component, oscillation component and light-shielding component.

[0009] Preferably, the oscillation assembly includes four groups of tension springs, a rotating motor, a rotating seat, a rotating shaft and a connecting seat. The four groups of tension springs are vertically installed in a centrally symmetrical manner between the bottom of the placement plate and the oscillation groove. The top of the rotating shaft is fixed at the center position of the bottom of the placement plate through the mounting seat. The bottom of the rotating shaft is fixedly inserted into one side of the connecting seat. The other side of the connecting seat is rotatably connected to the top of the rotating seat and is fixed by an adjusting bolt for angle limiting. The bottom of the rotating seat is fixedly connected to the output end of the rotating motor, and the rotating motor is fixedly installed at the bottom of the oscillation groove.

[0010] Preferably, the top of the rotating motor is connected and fixed to the bottom of the oscillation tank via two groups of fixing columns, and the rotating shaft of the rotating motor is not in a straight line.

[0011] Preferably, a collecting tank is fixedly provided inside the placement plate below the reaction tube, the top of the collecting tank is connected to the bottom of the reaction tube through a hose, the hose is opened and closed by an electromagnetic pinch valve, the bottom of the collecting tank is arranged as an inclined surface, a collecting port is provided at the bottom of the collecting tank and the bottom of the inclined surface, the collecting port is connected to a collecting bottle through a conducting pipe passing through the oscillation tank and the limit plate, the collecting bottle is arranged between the limit plate and the back plate of the cabinet, the top of the collecting bottle is closed and an exhaust port is also provided above it, the exhaust port is connected to a circulating water vacuum pump by passing through the outside of the back plate of the cabinet.

[0012] Preferably, the limiting plate is further provided with a partition plate above the collection bottle, the partition plate is provided with two groups of movable slide rails, a placing rack is slidably arranged on the movable slide rails, at least four groups of reagent bottle fixing seats are arranged on one side of the placing rack, reagent bottles are clamped in the reagent bottle fixing seats, a cleaning tank is arranged on the other side of the placing rack, a cleaning pump is arranged above the cleaning tank on the inner side of the placing rack, a plurality of exhaust ports are formed around the cleaning pump, the water inlet of the cleaning pump is communicated with an external pure water pipe, the cleaning pump is provided with a cleaning head, the water outlet of the cleaning head is arranged above the cleaning tank, the cleaning tank is connected with a cleaning bottle through a hose penetrating through the placing rack and the partition plate, and the cleaning bottle is arranged on one side of the collection bottle.

[0013] Preferably, the light-proof assembly comprises a fixed rod, a movable rod, light-proof cloth, a ball screw and a driving motor, the ball screw is provided with two groups, the two groups of ball screws are fixedly arranged in parallel on the inside of the cabinet top plate through a mounting base, the fixed rod is arranged in the inside of the cabinet top plate in vertical opposition to the mounting base, the movable rod is fixedly connected with the nuts on the ball screws at both ends, the light-proof cloth is sleeved between the fixed rod and the movable rod at both ends, the screw rods of the two groups of ball screws are in transmission connection with the driving motor through bevel gears at one end, and the driving motor is arranged in the inside of the cabinet top plate in vertical opposition to the ball screw.

[0014] Preferably, the light-proof cloth is arranged in a continuous honeycomb-like multi-layer state in the stretched state, the light-proof cloth is provided with non-woven fabric as an outer layer and aluminum foil as an inner layer, and the light-proof cloth is covered with heat insulation glass around.

[0015] Preferably, the sampling assembly comprises an XY-axis electric sliding table, a pneumatic sliding table and a sampling needle mechanism, the XY-axis electric sliding table is fixedly installed below the cabinet top plate, the pneumatic sliding table is vertically installed on the sliding seat of the XY-axis electric sliding table, and the sampling needle mechanism moves vertically up and down through the pneumatic sliding table.

[0016] Preferably, the lengths of the hoses between the collection tank and the bottom of the reaction tube and between the bottom of the cleaning tank and the cleaning bottle satisfy the required lengths of maximum strokes.

[0017] Preferably, the fixed port on the top of the placing disc is provided with a limiting rubber ring around the reaction tube.

[0018] In summary, the present application has the following beneficial effects:

[0019] The light-proof assembly provided with the electric opening and closing function can realize real-time understanding of the reaction tube and sampling in the device when the light-proof cloth is retracted, and can realize light shielding and heat preservation when the light-proof cloth is opened, so that the stable reaction in the device is ensured.

[0020] The present application can realize the oscillation movement of the placing disc through the setting of the oscillation assembly, compared with the gas mixing, the resin overflow caused by excessive gas can be avoided, meanwhile, the angle between the connecting seat and the rotating shaft is adjusted through the adjusting bolt, so that the 2D plane oscillation and the 3D oscillation with inclined angle are realized, so that the internal reagent can be better mixed, and the reaction efficiency of the reagent can be effectively improved.

[0021] The present application can realize the sampling, sampling and cleaning three steps flexibly through the setting of the sampling assembly, so that the sampling and mixing of multiple reagents in the sealed internal environment can be realized, and the reaction is not affected by multiple sampling.

[0022] The present application can realize the internal temperature stability through the setting of the two-layer heat insulation glass, the continuous honeycomb-shaped multilayer light-proof cloth and the air bath generator, and the sampling and reagent replacement can be facilitated through the oscillation groove and the placing rack arranged in the sliding mode. BRIEF DESCRIPTION OF DRAWINGS

[0023] Fig. 1 It is the overall structure schematic diagram of the present application;

[0024] Fig. 2 It is the structure diagram of the oscillation assembly in the present application under the 2D plane oscillation;

[0025] Fig. 3 It is the structure diagram of the oscillation assembly in the present application under the 3D oscillation with inclined angle;

[0026] Fig. 4 It is the structure schematic diagram of the placing rack in the present application;

[0027] Fig. 5 It is the position schematic diagram of the cleaning bottle and the collection bottle in the present application;

[0028] Fig. 6 It is the structure schematic diagram of the light-proof cloth in the present application after stretching;

[0029] Fig. 7 It is the structure schematic diagram of the light-proof assembly in the present application. DETAILED DESCRIPTION

[0030] The specific embodiments of the present application will be further described below in combination with the drawings, and the embodiments do not constitute the limitation of the present application.

[0031] As Figs. 1 to 7The constant temperature light-proof mixing device for polypeptide solid phase synthesis shown comprises a cabinet body 1, an oscillation tank 2, a control console 3, an air bath generator 4, an oscillation assembly 5 and a light-proof assembly 6. The control console 3 is fixedly installed on the top of the rear side of the cabinet body 1. An opening is arranged on the front side of the cabinet body 1. The oscillation tank 2 is slidably arranged in the cabinet body 1 through slide rails on both sides. A limiting plate 7 is arranged on the back of the oscillation tank 2 in the cabinet body 1. Gaps are arranged between both ends of the limiting plate 7 and the inner side walls of the cabinet body 1. Both sides of the oscillation tank 2 are connected with the inside of the cabinet body 1 through two groups of electric push rods 8 at both ends of the limiting plate 7. The electric push rods 8 are hingedly connected with the oscillation tank 2 and the inner wall of the cabinet body 1 at both ends. The oscillation assembly 5 is arranged on the inner bottom of the oscillation tank 2. A placing disc 9 is fixedly installed above the oscillation assembly 5. A plurality of fixing holes are arranged on the placing disc 9. A reaction tube 10 is inserted into each fixing hole. Limiting rubber rings 42 are arranged around the reaction tube 10 above the fixing hole of the placing disc 9. A window is arranged on the top of the control console 3. The window is sealedly connected through two groups of heat insulation glasses 11. The light-proof assembly 6 is fixedly installed between the two groups of heat insulation glasses 11. A sampling assembly 12 is arranged below the top of the cabinet body 1. The air bath generator 4 is fixedly installed on the top of the back of the cabinet body 1. The air bath generator 4 is communicated with the inside of the cabinet body 1. The control console 3 is electrically connected with the air bath generator 4, the sampling assembly 12, the oscillation assembly 5 and the light-proof assembly 6 for control. The air bath generator 4 adopts the prior art device. The air of a certain temperature is introduced to realize the control of the temperature in the cabinet.

[0032] The oscillation assembly 5 comprises four groups of tension springs 13, a rotating motor 14, a rotating seat 15, a rotating shaft 16 and a connecting seat 17. The four groups of tension springs 13 are vertically installed between the bottom of the placing disc 9 and the oscillation tank 2 in a central symmetry. The rotating shaft 16 is fixedly arranged on the bottom of the placing disc 9 through a mounting seat 18 at a central position. The rotating shaft 16 is fixedly inserted into one side of the connecting seat 17 at the bottom. The other side of the connecting seat 17 is rotatably connected with the upper side of the rotating seat 15 and is angularly limited and fixed through an adjusting bolt 19. The bottom of the rotating seat 15 is fixedly connected with the output end of the rotating motor 14. The rotating motor 14 is fixedly installed on the inner bottom of the oscillation tank 2. The rotating motor 14 is fixedly connected with the inner bottom of the oscillation tank 2 through two groups of fixing columns 43. The rotating shaft 16 of the rotating motor 14 is not in a straight line with the rotating shaft 16. The angle between the connecting seat 15 and the rotating shaft 16 is adjusted through the adjusting bolt 19. Thus, the 2D plane oscillation and the 3D oscillation with an inclined angle are realized. The amplitude of the 3D oscillation with an inclined angle is adjusted through the angle adjustment. Thus, the internal reagent can be better mixed. Thus, the reaction efficiency of the reagent is effectively improved.

[0033] A collection tank 20 is fixedly arranged inside the placing disc 9 below the reaction tube 10. The upper portion of the collection tank 20 is communicated with the bottom of the reaction tube 10 through a hose. The hose is conducted and closed through an electromagnetic pinch valve 21. The bottom of the collection tank 20 is arranged in a slope. A collection opening is arranged at the bottom of the collection tank 20 and the bottom of the slope. The collection opening is connected with a collection bottle 23 through a conducting pipe 22 penetrating the oscillation tank 2 and the limiting plate 7. The collection bottle 23 is arranged between the limiting plate 7 and the back plate of the cabinet 1. The top of the collection bottle 23 is closed. An air outlet is further arranged above the collection bottle 23. The air outlet is connected with a circulating water vacuum pump 24 penetrating the outside of the back plate of the cabinet 1. The waste liquid after reaction can be sucked into the collection bottle by opening the electromagnetic pinch valve 21 and the circulating water vacuum pump 24. Manual operation is not needed. It is more convenient.

[0034] A partition plate 25 is further fixedly arranged above the collection bottle 23. Two groups of movable sliding rails are arranged above the partition plate 25. A placing rack 26 is slidingly arranged on the movable sliding rails. At least four groups of reagent bottle fixing seats 27 are arranged on one side of the placing rack 26. The reagent bottles are clamped and arranged in the reagent bottle fixing seats 27. A cleaning tank 28 is arranged on the other side of the placing rack 26. A cleaning pump 29 is arranged on the inner side of the placing rack 26 above the cleaning tank 28. A plurality of air outlets are arranged around the cleaning pump 29. The air outlets are used for internal air circulation and internal pressure adjustment. The water inlet of the cleaning pump 29 is communicated with an external pure water pipe. The cleaning pump 29 is installed with a cleaning head 30. The water outlet of the cleaning head 30 is arranged above the cleaning tank 28. A cleaning bottle 31 is connected with the cleaning tank 28 through a hose penetrating the placing rack 26 and the partition plate 25. The cleaning bottle 31 is arranged on one side of the collection bottle 23. The cleaning pump 29 can clean the sampling assembly 12. The waste liquid enters the cleaning bottle 31 through the cleaning tank 28.

[0035] The light-proof assembly 6 comprises a fixed rod 32, a movable rod 33, a light-proof cloth 34, two groups of ball screws 35 and a driving motor 36. The two groups of ball screws 35 are fixedly arranged in parallel on the top plate of the cabinet 1 through a mounting base 37. The fixed rod 32 is arranged in the top plate of the cabinet 1 in a vertical manner relative to the mounting base 37. The movable rod 33 is fixedly connected with the nuts on the ball screws 35 at both ends. The light-proof cloth 34 is sleeved between the fixed rod 32 and the movable rod 33 at both ends. The screw rods of the two groups of ball screws 35 are drivingly connected with the driving motor 36 through bevel gears 38 at one end. The driving motor 36 is arranged in the top plate of the cabinet 1 in a vertical manner relative to the ball screws 35. The light-proof cloth 34 is arranged in a continuous honeycomb-like multi-layer state in a stretched state. The outer layer of the light-proof cloth 34 is non-woven fabric. The inner layer is attached with aluminum foil. The light-proof cloth 34 is covered with the heat insulation glass 11 around. The light-proof cloth 34 can be used to realize real-time understanding of the reaction tube and sampling in the device when the light-proof cloth 34 is retracted. The light-proof cloth 34 can realize light shielding and heat preservation when opened. The stable reaction in the device is ensured.

[0036] The sampling assembly 12 comprises an XY-axis electric sliding table 39, a pneumatic sliding table 40 and a sampling needle mechanism 41. The XY-axis electric sliding table 39 is fixedly installed below the top plate of the cabinet 1. The pneumatic sliding table 40 is vertically installed on the sliding seat of the XY-axis electric sliding table 39. The sampling needle mechanism 41 vertically moves up and down through the pneumatic sliding table 40. The sampling needle mechanism 41 is a prior art and can realize sample suction and injection. At the same time, the sampling needle mechanism 41 realizes XYZ three-axis movement through the XY-axis electric sliding table 39 and the pneumatic sliding table 40, covering the reaction tube 10, the reagent bottle seat 27 and the cleaning tank 28, thereby flexibly realizing the sampling, sample injection and cleaning steps, so as to realize the sample injection mixing of multiple reagents in a sealed internal environment and avoid the influence of multiple sample injection on the reaction.

[0037] The lengths of the hoses between the collection tank 20 above and the bottom of the reaction tube 10 and between the bottom of the cleaning tank 28 and the cleaning bottle 31 meet the length required by the maximum stroke, so as to avoid the influence on the collection of waste liquid and cleaning liquid in the opening process.

[0038] The above is only a preferred embodiment of the present application and is not used to limit the present application. Those skilled in the art can make various modifications or equivalent replacements to the present application within the spirit and protection scope of the present application. The modification or equivalent replacement is also regarded as falling within the protection scope of the technical scheme of the present application.

Claims

1. A thermostatic light-protected mixing device for solid phase synthesis of polypeptides, characterized in that, The utility model provides a kind of gas bath generator and sampling device, including cabinet, oscillation tank, console, gas bath generator, oscillation subassembly and light-proof component, the console is fixedly installed in the rear side above cabinet top plate, the cabinet front side is provided with opening, the oscillation tank both sides are slidably arranged in the inside of cabinet by slide rail, the inside of cabinet is provided with a limit plate in the back of oscillation tank, the limit plate both ends are provided with gap with the inside lateral wall of cabinet, the oscillation tank both sides are connected with the inside of cabinet at the both ends of limit plate by two groups of electric push rod, the electric push rod both ends are hinged with oscillation tank, the inside bottom of oscillation tank is provided with oscillation subassembly, the oscillation subassembly is fixedly installed with placing tray above, the placing tray is provided with multiple fixed ports, and reaction tube is inserted in each fixed port, the cabinet top plate is provided with a window in the front side of console, the window is sealedly connected by two groups of heat insulation glass from top to bottom, the light-proof component is fixedly installed between two groups of heat insulation glass, the cabinet top plate is provided with a sampling component below, the gas bath generator is fixedly installed on the back of cabinet above, the gas bath generator is communicated with the inside of cabinet, the console is electrically connected with gas bath generator, sampling component, oscillation subassembly and light-proof component control; The oscillation subassembly includes four groups of tension springs, a rotating motor, a rotating seat, a rotating shaft and a connecting seat. The four groups of tension springs are vertically installed between the bottom of the placing tray and the oscillation tank in a central symmetrical manner. The rotating shaft is fixedly arranged at the center position of the bottom of the placing tray through the mounting seat. The bottom of the rotating shaft is fixedly inserted into one side of the connecting seat. The other side of the connecting seat is rotatably connected with the top of the rotating seat and is angularly limited and fixed by the adjusting bolt. The bottom of the rotating seat is fixedly connected with the output end of the rotating motor. The rotating motor is fixedly installed in the bottom of the oscillation tank. The light-proof component includes a fixed rod, a moving rod, a light-proof cloth, two groups of ball screws and a driving motor. The two groups of ball screws are fixedly arranged in the inside of the cabinet top plate in parallel through the mounting base. The fixed rod is vertically arranged in the inside of the cabinet top plate opposite to the mounting base. The two ends of the moving rod are fixedly connected with the nuts on the ball screws. The light-proof cloth is sleeved between the fixed rod and the moving rod. The screw rods of the two groups of ball screws are drivingly connected with the driving motor through the helical gears. The driving motor is vertically arranged in the inside of the cabinet top plate opposite to the ball screws.

2. The constant temperature, light-free mixing device for the solid phase synthesis of polypeptides of claim 1, wherein: The rotating motor is fixedly connected with the bottom of the oscillation tank through two groups of fixed columns. The rotating shaft of the rotating motor is not in a straight line with the rotating shaft.

3. The constant temperature, light-free mixing device for the solid phase synthesis of polypeptides of claim 1, wherein: A collecting tank is fixedly arranged in the inside of the placing tray below the reaction tube. The top of the collecting tank is communicated with the bottom of the reaction tube through a hose. The hose is conducted and closed by the electromagnetic pinch valve. The bottom of the collecting tank is provided with a slope. The bottom of the collecting tank and the bottom of the slope are provided with a collecting port. The collecting port is connected with a collecting bottle through a conducting pipe penetrating the oscillation tank and the limit plate. The collecting bottle is arranged between the limit plate and the back plate of the cabinet. The top of the collecting bottle is closed and is further provided with an air outlet. The air outlet is connected with a circulating water vacuum pump penetrating the outside of the back plate of the cabinet.

4. The constant temperature, light-free mixing device for the solid phase synthesis of polypeptides of claim 1, wherein: The limiting plate is also fixedly provided above the collecting bottle, a partition plate is arranged above the limiting plate, two groups of movable slide rails are arranged above the partition plate, a placing rack is slidably arranged on the movable slide rails, at least four groups of reagent bottle fixing seats are arranged on one side of the placing rack, reagent bottles are clamped and arranged in the reagent bottle fixing seats, a cleaning tank is arranged on the other side of the placing rack, a cleaning pump is arranged above the cleaning tank on the inner side of the placing rack, a plurality of exhaust ports are formed around the cleaning pump, a water inlet of the cleaning pump is communicated with an external pure water pipe, a cleaning head is installed on the cleaning pump, a water outlet of the cleaning head is arranged above the cleaning tank, a cleaning bottle is connected to the partition plate through a hose penetrating the placing rack from the bottom of the cleaning tank to one side of the collecting bottle.

5. The constant temperature, light-free mixing device for the solid phase synthesis of polypeptides of claim 1, wherein: The light-proof cloth is continuously and multi-layered in a honeycomb shape in a stretched state, the outer layer of the light-proof cloth is a non-woven fabric, the inner layer is attached to an aluminum foil, and the light-proof cloth is covered with heat insulation glass around.

6. The thermostatted light-tight mixing device for the solid phase synthesis of polypeptides according to claim 1, characterized in that The sampling assembly comprises an XY-axis electric sliding table, a pneumatic sliding table and a sampling needle mechanism, the XY-axis electric sliding table is fixedly installed below the top plate of the cabinet, the pneumatic sliding table is vertically installed on the sliding seat of the XY-axis electric sliding table, and the sampling needle mechanism vertically moves up and down through the pneumatic sliding table.

7. The constant temperature, light-free mixing device for the solid phase synthesis of polypeptides of claim 3, wherein: The lengths of the hoses between the collecting tank and the bottom of the reaction tube and between the bottom of the cleaning tank and the cleaning bottle satisfy the required length of the maximum stroke.

8. The constant temperature, light-free mixing device for the solid phase synthesis of polypeptides of claim 1, wherein: The limiting rubber rings are arranged around the reaction tube above the fixed port of the placing disc.

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