Microfluidic switching valve, multi-sample injection analysis system, and multi-sample analysis method

Abstract

The present application provides a kind of microfluidic switching valve and multi-sample injection analysis system, microfluidic switching valve includes first valve body and second valve body, first valve body is provided with a first interface, a plurality of second interfaces and a third interface, second valve body is provided with first channel and second channel, multi-sample injection analysis system includes injection device, microfluidic switching valve, analysis equipment, cleaning fluid supply device;First interface is communicated with analysis equipment;First interface can be selectively communicated with any one of second interface or third interface, other second interfaces and / or third interfaces not communicated with the inlet of first interface are mutually communicated with each other;Injection device has multiple sample outlets, each sample outlet is communicated with a second interface correspondingly.This microfluidic switching valve of the present application has less interface, and can carry out pipeline cleaning after injection, reduce the cross contamination between samples.

Description

[0001] This application claims priority to the invention patent application filed on March 11, 2022, with application number 202220540283.9 and entitled "Microfluidic Switching Valve and Multi-Sample Injection Analysis System", the entire contents of which are incorporated herein by reference. Technical Field

[0002] This invention relates to the field of analytical instrument technology, specifically to a microfluidic switching valve, a multi-sample injection analysis system using the microfluidic switching valve, and a multi-sample injection analysis method for the multi-sample injection analysis system. Background Technology

[0003] like Figure 1 As shown, Chinese patent document (CN110988228A) discloses an automated multi-sample injection analysis device, including a sampling device and a liquid chromatography device. The sampling device includes a multi-channel microfluidic switching valve connected to the liquid chromatography device via an outlet pipe. If six microchannels need to be detected, this fluid switching valve requires a total of 13 pipe interfaces. Every two interfaces (g with 1', 3', 5', 7', 9', or 11') form a sample inflow channel, and every two interfaces form a temporary receiving channel for non-detection samples (1' with 2', 3' with 4', 5' with 6', 7' with 8', 9' with 10', 11' with 12'). There is a sample selection channel in the middle for selecting samples to enter the downstream liquid chromatography device. The excessive number of pipe interfaces increases the difficulty of valve plate manufacturing, and the piping used is also relatively complex, resulting in higher costs.

[0004] In addition, in previous multi-sample automated sample analysis devices, when each sample entered the downstream liquid chromatography unit in turn, it was impossible to clean the tubing between the multi-channel microfluidic switching valve and the liquid chromatography unit, which easily led to cross-contamination between samples. Summary of the Invention

[0005] To address the above problems, the first aspect of this invention provides a microfluidic switching valve with fewer pipe interfaces, lower manufacturing cost, and the ability to clean the pipes of other samples after sample injection, as well as a multi-sample injection analysis system.

[0006] To address the aforementioned technical problems, this invention provides a microfluidic switching valve for a multi-sample injection analysis system, comprising:

[0007] First valve body;

[0008] The first interface is located at the center of the first valve body;

[0009] Multiple second interfaces are arranged on the first valve body in a manner that surrounds the first interface;

[0010] A third interface is provided on the first valve body in the same form as the second interface;

[0011] A first channel that is connected to a first interface at one end and can be connected to any one of a plurality of second interfaces or a third interface at the other end, and is not connected to the other plurality of second interfaces or third interfaces.

[0012] The second channel is a combination of multiple second and / or third interfaces that are not connected to the first channel, and is connected to each other.

[0013] According to the technical solution of the present invention, since one end of the first channel is connected to the first interface, and the other end can be selectively connected to any one of the plurality of second interfaces or the third interface, and is not connected to the other plurality of second interfaces or the third interface, and the plurality of second interfaces are arranged around the first interface on the first valve body, when the first interface of the microfluidic switching valve is connected to one of the plurality of second interfaces through the first channel, the sample entering from the outside through the second interface flows through the first channel and flows out from the first interface, for example, to the downstream liquid chromatography device for detection. At this time, since the second channel connects all the other second interfaces (including the third interface), other samples entering the microfluidic switching valve from the outside through any other second interface can flow through the second channel to a designated location, for example, they can be discharged to the waste liquid collection device through the third interface. In this way, when the first interface of the microfluidic switching valve is connected to the second interfaces one by one through the first channel, the samples entering from the outside through the second interface can be detected one by one, and the detection of multiple samples can be completed.

[0014] Therefore, compared with the prior art, each second interface of the microfluidic switching valve of the present invention can form a sample inflow channel, thus achieving switching of multiple sample detection with a smaller number of interfaces. The microfluidic switching valve has a simple structure and low manufacturing cost.

[0015] In an optional technical solution of the present invention, a plurality of second interfaces are arranged in a ring shape and at equal intervals on the cross-section of the first valve body; and a third interface is arranged in the same ring as the second interfaces and at equal intervals from the second interfaces.

[0016] According to this optional technical solution, since multiple second interfaces, including the third interface, are arranged at equal intervals around the first interface on the cross-section of the first valve body, communication between the first channel and any of the second or third interfaces can be achieved simply by rotating and adjusting the relative positions of the first channel and the second interfaces with the first interface as the center. The microfluidic switching valve has a simple structure and is easy to operate.

[0017] In an optional technical solution of the present invention, the first interface is connected to the inlet of the analytical device, and multiple second interfaces are connected to the outlet of the sample injection device. According to this technical solution, each of the multiple second interfaces of the microfluidic switching valve is connected to the outlet of the sample injection device to receive multiple samples flowing in from the outlet of the sample injection device, while the sample flowing through the first interface flows into the inlet of the analytical device for detection, thereby realizing the individual detection of multiple samples.

[0018] In an optional technical solution of the present invention, the third interface is connected to a cleaning fluid supply device and / or a waste liquid collection device. According to this solution, when the third interface is connected to the waste liquid collection device, non-detection samples flowing into other second interfaces can be collected through the second channel and then flow into the waste liquid collection device via the third interface. When the third interface is connected to the cleaning fluid supply device, the cleaning fluid can flow into and through the first channel and the first interface, as well as the pipeline between the first interface and the analytical device, thereby cleaning the pipeline and avoiding cross-contamination between samples. With a simple structure, this improves detection accuracy and efficiency.

[0019] The present invention also provides a multi-sample injection analysis system, which includes the microfluidic switching valve of any of the above-mentioned technical solutions, and further includes an injection device, an analytical device, and a cleaning fluid supply device, wherein the first interface is connected to the inlet of the analytical device; the injection device has multiple sample outlets, each sample outlet being connected to a corresponding second interface; and the third interface is connected to the cleaning fluid supply device.

[0020] According to the technical solution of the present invention, when the first channel of the microfluidic switching valve is switched to be connected to the second interface corresponding to the currently detected sample, among multiple samples from the sample introduction device, the currently detected sample flows into the microfluidic switching valve through its corresponding second interface, and after passing through the first channel and the first interface, flows into the inlet of the analytical device for detection. Other samples not currently being detected flow into the microfluidic switching valve through other second interfaces not connected to the first interface, and after passing through interconnected second channels, exit the microfluidic switching valve through the third interface, thus recovering the samples not currently being detected.

[0021] Compared to existing technologies that add interfaces to form a sample outflow channel with two interfaces, where samples enter through the inflow channel and exit through the outflow channel, the microfluidic switching valve and the multi-sample injection analysis system using this invention significantly reduce the number of interfaces on the microfluidic switching valve, lowering the processing difficulty and manufacturing cost. Furthermore, by providing a third interface connected to the cleaning fluid supply device, after completing the current sample test and before preparing to test the next sample, switching the microfluidic switching valve to connect the first and third interfaces allows the cleaning fluid from the supply device to flow from the third interface through the first channel and the first interface, then into the pipeline between the first interface and the analysis equipment, cleaning this part of the pipeline and preventing cross-contamination between multiple samples that could affect detection accuracy. This invention simplifies the structure of the multi-sample injection microfluidic switching valve, reduces the number of interfaces at both ends of the flow channel, and lowers the processing difficulty and production cost of the microfluidic switching valve. Meanwhile, while enabling the injection and detection of multiple samples, the simple structure facilitates pipeline cleaning after sample testing, which in particular reduces cross-contamination of the pipeline between the first interface and the inlet of the analytical equipment when multiple samples are injected for testing, thereby improving detection efficiency and accuracy.

[0022] In an optional technical solution of the present invention, the multi-sample injection analysis system further includes a second switching valve, which is disposed on the pipeline between the outlet of the cleaning fluid supply device and the inlet of the third interface. According to this technical solution, the cleaning fluid from the cleaning fluid supply device can be delivered to the inlet of the analytical device via the second switching valve, the third interface, the first interface, and so on. This simple flow path structure enables cleaning of the pipeline between the analytical device and the microfluidic switching valve, reducing the manufacturing difficulty and production cost of the multi-sample injection analysis system.

[0023] In an optional technical solution of the present invention, a waste liquid collection device is also included, and the second switching valve further includes a valve body opening connected to the waste liquid collection device. The second switching valve can selectively switch the third interface to either the cleaning fluid supply device or the waste liquid collection device.

[0024] According to this technical solution, a second switching valve can be used to connect the third interface to the cleaning fluid supply device or the waste liquid collection device with a relatively simple flow path structure, thereby achieving both cleaning and waste liquid recovery. The structure is simple and the cost is low.

[0025] In an optional technical solution of the present invention, the analytical device is a liquid chromatography apparatus.

[0026] According to this technical solution, for multiple samples to be tested, a liquid chromatography device can be used to inject and test each sample one by one, and the samples can be cleaned after each test, which improves the cleanliness of liquid chromatography injection, reduces cross-contamination, and improves the testing accuracy of the liquid chromatography device.

[0027] In an optional technical solution of the present invention, the sample introduction device includes a microfluidic injection pump and a microfluidic chip. The microfluidic chip has multiple sample channels, the inlet of each sample channel is connected to the outlet of the microfluidic injection pump, and the outlet of each sample channel is connected to a second interface. According to this technical solution, because a microfluidic injection pump and a microfluidic chip are used, the microfluidic chip has the characteristics of controllable liquid flow, minimal consumption of sample and reagents, and significantly improved analysis speed. Therefore, the multi-sample introduction analysis system of the present invention can be applied to many high-precision detection and analysis fields such as biology, chemistry, and medicine.

[0028] In an optional embodiment of the present invention, the microfluidic chip is disposed within a microfluidic chip mounting box. According to this embodiment, the microfluidic chip being disposed within the mounting box ensures that the microfluidic chip can operate precisely, stably, and reliably.

[0029] A second aspect of the present invention provides a multi-sample injection analysis system having the above-mentioned microfluidic switching valve and a multi-sample injection analysis method for the multi-sample injection analysis system, which can simplify the pipeline and clean the sample after injection, and store the sample in advance to further reduce the multi-sample injection and detection time.

[0030] The second aspect of the present invention provides a multi-sample injection analysis system including the microfluidic switching valve of any of the above-described inventive solutions, and further including an injection device, multiple sample loops, an analytical device, a buffer supply device, and a buffer flow path switching device, wherein the injection device has multiple sample outlets, each sample outlet being connected to a second interface via a sample loop; the inlet of the analytical device is connected to a first interface; and the buffer flow path switching device is used to switch the buffer supply device to the inlet of different sample loops.

[0031] According to this technical solution, the flow path from the sample outlet of the injection device to the microfluidic switching valve is the injection flow path. Each injection flow path is equipped with a sample loop. When the injection device injects sample into the microfluidic switching valve, multiple sample loops can simultaneously accumulate sample. After the sample accumulation in multiple sample loops is complete, the first channel of the microfluidic switching valve is switched to the second interface corresponding to the currently detected sample. Simultaneously, the sample loop inlet is switched to connect with the buffer supply device. Buffer enters the sample loop through the inlet, pushing the accumulated sample in the sample loop into the analytical device for detection. The continuously flowing buffer also cleans the tubing from the sample loop to the analytical device after detection. After detection, the buffer supply device is switched to connect to the inlet of another sample loop that has accumulated sample, repeating the above detection process. Simultaneously, the injection device continues to accumulate sample in the sample loops that have completed detection.

[0032] Therefore, the multi-sample injection analysis system provided by the second aspect of the present invention, compared with the prior art and the multi-sample injection analysis system provided by the first aspect, can not only achieve switching of multi-sample detection with fewer interfaces and lower manufacturing cost, but also can simultaneously carry out the sample accumulation process through sample loops on multiple injection flow paths, reducing multi-sample detection time and sample waste.

[0033] In an optional technical solution of the present invention, the buffer flow path switching device includes a plurality of third switching valves, which are respectively disposed between the sample outlet and the inlet of the sample loop, for independently switching the inlet of each sample loop to the sample outlet or to the buffer supply device.

[0034] According to this technical solution, when a third switching valve is switched to connect the sample inlet and the sample outlet of the injection device, the injection device of this injection path injects sample into the sample loop. The sample accumulates in the sample loop until the sample volume in the sample loop reaches the detection volume. Then, the third switching valve is switched to connect the sample inlet and the buffer supply device. The buffer supply device injects buffer into the sample loop, pushing the sample accumulated in the sample loop into the analytical device for detection. Therefore, sample accumulation and sample cleaning operations can be achieved through a simple flow path switching.

[0035] In an optional technical solution of the present invention, the buffer supply device includes a fourth switching valve, the fourth switching valve having a fourth interface disposed at the center of the fourth switching valve; a plurality of fifth interfaces disposed around the fourth interface; a third channel having one end connected to the fourth interface and the other end optionally connected to any one of the plurality of fifth interfaces, and not connected to the other plurality of fifth interfaces; the plurality of fifth interfaces of the fourth switching valve being respectively connected to the plurality of third switching valves; and the fourth interface being connected to the buffer inlet.

[0036] According to this technical solution, the buffer solution flows into the buffer supply device through the fourth port of the fourth switching valve, and multiple fifth ports surround the fourth port. The third channel formed by the connection between the fourth and fifth ports is the buffer supply path. When the buffer supply device is connected to the sample loop, the fourth port connects to the corresponding fifth port of the sample loop, forming the buffer supply path. The buffer solution flows to the sample loop through the buffer supply path, pushing the samples in the sample loops on different paths into the analytical device. The control and switching of the flow of buffer solution into different sample loops can be achieved with a single valve body, simplifying the device structure.

[0037] In an optional technical solution of the present invention, the sample injection device further includes a microfluidic injection pump and a microfluidic chip. The microfluidic chip has multiple sample channels, and the inlet of each sample channel is connected to the outlet of the microfluidic injection pump.

[0038] According to this technical solution, because it uses a microfluidic injection pump and a microfluidic chip, the microfluidic chip has the characteristics of controllable liquid flow, minimal consumption of samples and reagents, and significantly improved analysis speed. Therefore, the multi-sample injection analysis system of this invention can be applied to many high-precision detection and analysis fields such as biology, chemistry, and medicine.

[0039] The second aspect of this invention provides a multi-sample injection analysis method that can be used in any of the multi-sample injection analysis systems described above. This multi-sample injection analysis method includes the following steps:

[0040] In the liquid storage step, the sample enters through multiple sample outlets and fills multiple sample loops;

[0041] In the sample introduction step, the buffer supply device is switched one by one to the inlet of different sample loops, and the buffer solution is used to push the sample in each sample loop into the analysis device.

[0042] The testing procedure involves using analytical equipment to test and analyze the sample.

[0043] According to this technical solution, firstly, by using sample loops on multiple injection paths to simultaneously perform the sample accumulation process, the detection time for multiple samples can be effectively reduced; secondly, by using buffer solution to push the sample in the sample loop into the analytical device, the tubing can be directly cleaned with buffer solution after sample injection, without the need for other switching operations or separate cleaning structures, thus simplifying the steps and apparatus for multiple sample detection.

[0044] In an optional technical solution of the present invention, in the sample injection step, after the sample loop is injected, a cleaning step is also performed, in which buffer solution is continuously introduced into the inlet of the sample loop to clean the injection and detection tubing.

[0045] According to this technical solution, continuous flow of buffer solution after sample injection can directly clean the tubing without the need for switching operations or additional cleaning solution devices, making it simple and quick.

[0046] In an optional technical solution of the present invention, if the plurality of sample rings includes at least one unfilled sample ring and at least one filled sample ring, the liquid storage step for the unfilled sample ring and the sample injection step for the filled sample ring are performed simultaneously.

[0047] According to this technical solution, the sample injection step for an incomplete sample loop and the liquid storage step for a completed sample loop are performed simultaneously, which can further save time for multi-sample detection. Attached Figure Description

[0048] Figure 1 This is a schematic diagram of the structure of a microfluidic switching valve in the prior art.

[0049] Figure 2 This is a schematic diagram of the structure of the multi-sample injection analysis system in the first embodiment of the present invention.

[0050] Figure 3 This is a schematic diagram of the microfluidic switching valve in the first embodiment of the present invention.

[0051] Figure 4 This is a schematic diagram of the sample detection flow path at interface 1 in the first embodiment of the present invention.

[0052] Figure 5 This is a schematic diagram of the sample detection flow path at interface 2 in the first embodiment of the present invention.

[0053] Figure 6 This is a schematic diagram of the pipeline cleaning flow path of the multi-sample injection analysis system in the first embodiment of the present invention.

[0054] Figure 7 This is a schematic diagram of the structure of the multi-sample injection analysis system provided in the second embodiment of the present invention.

[0055] Figure 8 This is a schematic diagram of another multi-sample injection analysis system provided in the second embodiment of the present invention.

[0056] Figure 9 This is a flowchart of the multi-sample injection analysis method provided in the second embodiment of the present invention.

[0057] Figure 10 This is a schematic diagram of the multi-sample injection analysis system provided in this embodiment of the invention detecting sample ring #1.

[0058] Figure 11 This is a schematic diagram of the multi-sample injection analysis system provided in this embodiment of the invention detecting sample ring #2.

[0059] Figure label:

[0060] Sample introduction device 1; microfluidic injection pump 11; microfluidic chip mounting box 12; microfluidic chip 121; sample flow channel 122; microfluidic switching valve 2; first valve body 21; first interface 211; second interface 212; third interface 213; first channel 214; second channel 215; second valve body 22; analytical device 3; cleaning solution supply device 4; second switching valve 5; waste liquid collection device 6; sample loop 7; buffer supply device 8; fourth switching valve 81; fourth interface 811; fifth interface 812; third channel 814; buffer injection pump 82; buffer flow path switching device 9; third switching valves 91, 92, 93, 94, 95; sample introduction flow path 100. Detailed Implementation

[0061] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0062] First Implementation Method

[0063] Some existing microfluidic systems used for cell analysis (such as the microfluidic system provided by CN110988228A) require the culture medium containing drugs to be slowly injected into the microfluidic chip using a microfluidic pump or nanofluidic pump. Each sample channel 122 of the microfluidic chip contains cells to be tested. To ensure cell survival, the culture medium needs to be supplied to the microfluidic chip almost continuously.

[0064] See Figure 2 and Figure 3 As shown, the first embodiment of the present invention provides a microfluidic switching valve applicable to a cell analysis microfluidic system and a multi-sample injection analysis system using the microfluidic switching valve, which can realize the automatic sample injection analysis function of the microfluidic system and ensure that the culture medium is supplied to the microfluidic chip with minimal interruption. The multi-sample injection analysis system provided in this embodiment includes an injection device 1, a microfluidic switching valve 2, and an analysis device 3. The microfluidic switching valve 2 includes a first valve body 21 and a first interface 211 provided on the first valve body 21. Figure 2 The S-interface shown), and multiple second interfaces 212 ( Figure 2 Interfaces 1, 2, 3, 4, and 5 (as shown) and a third interface 213 ( Figure 2 The W interface shown is connected to the output of the first interface 211, which is connected to the analysis device 3.

[0065] like Figure 3 As shown, the microfluidic switching valve 2 includes a first valve body 21 and a second valve body 22 that are arranged opposite to each other and rotatable relative to each other. A first interface 211, multiple second interfaces 212, and a third interface 213 are disposed on the first valve body 21. On the second valve body 22, which is arranged opposite to the first valve body 21, a first channel 214 extends radially from the center, and a second channel 215 is also disposed on the second valve body 22 in an open annular shape surrounding the first channel 214. The first channel 214 and the second channel 215 can, for example, be an open groove disposed on the second valve body 22. When the relative rotational position of the first valve body 21 and the second valve body 22 causes the two ends of the first channel 214 to coincide with the first interface 211 and the second interface 212 (e.g., interface 1), the first interface 211 and the second interface 212 are connected through the first channel 214.

[0066] The annular opening formed by the second flow channel 215 can correspondingly cover multiple second ports 212 and third openings 213 provided on the first valve body 21. However, the number of second ports 212 and third openings 213 that can be covered is at least one less than the total number of second ports 212 and third openings 213 provided on the first valve body 21. For example Figure 3 As shown, the first valve body 21 has six ports in total, including five second ports 212 and one third port 213, while the second valve body 22 has an open annular second channel 215 that can only cover five ports.

[0067] By rotating either the first valve body 21 or the second valve body 22, the first interface 211 can selectively connect to one of the second interfaces 212 via the first channel 214. Figure 2 The interface shown is connected to either interface 1 or interface 213, while other interfaces 212 not connected to interface 211 are as follows: Figure 2 The interfaces 2, 3, 4, 5 and / or the third interface 213 shown are interconnected through the second channel 215; the sample injection device 1 has multiple sample outlets, each of which is connected to the inlet of a second interface 212 of the microfluidic switching valve 2.

[0068] As a preferred embodiment of the present invention, the multi-sample injection analysis system of the present invention may further include a cleaning fluid supply device 4 and a second switching valve 5, wherein the cleaning fluid supply device 4 is connected to the third interface 213 through the second switching valve 5. Specifically, the second switching valve 5 has at least two valve body openings, which are respectively connected to the outlet of the cleaning fluid supply device 4 and the inlet of the third interface 213.

[0069] In a preferred embodiment of the present invention, the multi-sample injection analysis system of the present invention may further include a waste liquid collection device 6, the inlet of which is connected to a third interface 213 via a second switching valve 5. Simultaneously, the inlet of the waste liquid collection device 6 may also be connected in a bypass manner to the inlet of the analysis device 3. Alternatively, the inlet of the waste liquid collection device 6 may be directly connected to the third interface 213 without using the second switching valve 5.

[0070] In this embodiment of the invention, the outlet of the first interface 211 is connected to the inlet of the analysis device 3 via a pipeline. During sample injection, the sample to be tested, i.e., the current sample to be tested, is introduced from the second interface 212. Figure 2 As shown, the fluid flows into the microfluidic switching valve 2 through port 1, and after passing through the first channel 214, it exits from the first port 211 ( Figure 2 The contents of the S-interface flow out and enter the analysis device 3 through the pipeline for detection.

[0071] like Figure 2 As shown, because one end of the first channel 214 is connected to the first interface 211, and the other end is connected to interface 1 (second interface 212), and is connected to multiple other second interfaces 212 ( Figure 2 Interfaces 2, 3, 4, and 5 (as shown) and the third interface 213 are all in a disconnected state. At this time, because the second channel 215 connects the other multiple second interfaces 212 ( Figure 2 Interfaces 2, 3, 4, and 5 (as shown) and the third interface 213 are all connected. Therefore, when detecting the current sample flowing in through interface 1, it can be connected through any of the other second interfaces 212 ( Figure 2 Other samples that enter the microfluidic switching valve 2 from the outside (through the No. 2, No. 3, No. 4, or No. 5 interface shown) can be discharged to the waste liquid collection device 6 through the third interface 213 after being merged through the second channel 215.

[0072] like Figure 2 and Figure 3 As shown, multiple second interfaces 212 are arranged around the first interface 211 on the first valve body 21. Therefore, rotating the first valve body 21 or the second valve body 22 allows the first interface 211 of the microfluidic switching valve 2 to connect to any one of the second interfaces 212 through the first channel 214. Figure 2 When interfaces 2, 3, 4, or 5 are connected one by one, samples entering from the outside through the second interface 212 can be detected one by one, thus completing the detection of multiple samples.

[0073] Therefore, the microfluidic switching valve 2 provided by this invention has a simple structure. Sample inflow and outflow can be achieved simply by connecting the second interface 212 and the first interface 211. The sample in the injection channel of the second interface 212 directly enters the analysis device 3 for sample detection through the intermediate hub function of the first interface 211, improving sample injection efficiency and reducing the number of interfaces. Compared with the prior art, which requires a total of 13 pipe interfaces for detecting 6 microchannels, the microfluidic switching valve 2 of this invention only requires 7 pipe interfaces for detecting 5 samples, greatly simplifying the structure of the microfluidic switching valve, reducing processing difficulty, and lowering manufacturing costs.

[0074] Meanwhile, other second interfaces 212 and / or third interfaces 213 that are not connected to the first interface 211 collect non-currently detected samples through the interconnected second channels 215 and discharge them directly through the third interfaces 213, thus achieving the recovery of non-currently detected samples. The discharged non-currently detected samples can flow through the second switching valve 5 and then into the waste liquid collection device 6.

[0075] After the current sample is tested, when pipeline cleaning is required, the first valve body 21 or the second valve body 22 is rotated to connect the first port 211 and the third port 213 through the first channel 214. The cleaning fluid then flows from the cleaning fluid supply device 4, passes through the second switching valve 5, enters the third port 213, and flows out from the outlet of the first port 211. It then flows into the pipeline between the first port 211 and the inlet of the analytical device 3, thus cleaning the internal flow path of the microfluidic switching valve 2 and the pipeline between it and the inlet of the analytical device 3. The cleaning fluid, after cleaning, flows into the waste liquid collection device 6 through the bypass pipeline between the cleaning fluid and the inlet of the analytical device 3.

[0076] In this way, the cleaning solution can flow into the interior of the microfluidic switching valve 2 through the third interface 213, and flow through the first channel 214 and the first interface 211, as well as the pipeline between the first interface 211 and the analytical device 3, thereby cleaning the pipeline and avoiding cross-contamination between samples in the pipeline between the first interface 211 and the analytical device 3 when multiple channels are used for sample injection. With the simple structure of the microfluidic switching valve 2, the detection accuracy and detection efficiency are improved.

[0077] In a preferred embodiment of the present invention, the multi-sample injection analysis system has a sample detection flow path, which is sequentially connected to the injection device 1, the second interface 212, the first channel 214, the first interface 211, and the analysis device 3. For example... Figure 4 The diagram shown is a schematic diagram of the sample detection flow path at interface 1 in an embodiment of the present invention. Figure 5 The diagram shown is a schematic diagram of the sample detection flow path at interface 2 in an embodiment of the present invention.

[0078] Figure 4 and Figure 5 The detection and cleaning operations for sample detection flow paths at interfaces 1 and 2 are explained in detail below, in conjunction with the usage method of the multi-sample injection analysis system.

[0079] At the start of use, by rotating and adjusting the microfluidic switching valve 2, the first port 211 (port S in the diagram) and the second port 212 (port 1 in the diagram) of the microfluidic switching valve 2 are connected through the first channel 214. The other second ports 211 (ports 2-5 in the diagram) and the third port 213 (port W in the diagram) are connected to each other through the second channel 215. Then, switch the second switching valve 5 to connect it to the waste liquid collection device 6. At this time, the current sample to be tested from the sample injection device 1 enters port 1 and flows into the analysis device 3 for testing through the first port 211 (port S). Other samples not currently being tested that flow into ports 2-5 are discharged into the waste liquid collection device 6 through the second channel 215 and then through the third port 213 (port W).

[0080] After the sample flowing through interface 1 is detected, the first valve body 21 of the microfluidic switching valve 2 remains stationary, while the second valve body 22 rotates counterclockwise by 60° as shown in the figure. This connects the first interface 211 (S interface) of the microfluidic switching valve 2 with the third interface 213 (W interface). At this time, the second switching valve 5 is switched to connect with the cleaning fluid supply device 4. The cleaning fluid is then supplied through the second switching valve 5 via the cleaning fluid supply device 4, injected into the third interface (W interface in the figure) of the microfluidic switching valve 2, and then flows through the first channel 214 and through the first interface 211 (S interface in the figure) to the inlet of the analytical device 3. This cleans the tubing within the microfluidic switching valve 2, as well as the tubing between the first interface 211 and the inlet of the analytical device 3. The cleaning fluid, after tubing cleaning, flows from the bypass before the inlet of the analytical device 3 into the waste liquid collection device 6.

[0081] After cleaning, the first valve body 21 of the microfluidic switching valve 2 remains stationary, while the second valve body 22 rotates 120° clockwise as shown in the figure. At this time, as... Figure 5 As shown, the first interface 211 (S interface) is connected to interface 2 (shown in the diagram) of the second interface 212, while interfaces 1, 3, 4, and 5, and the third interface 213 (W interface) are connected through the second channel 215. At this time, the second switching valve 5 is switched to connect with the waste liquid collection device 6. In this state, the next sample to be tested from the sample introduction device 1, corresponding to the sample at interface 2, enters interface 2 and flows into the analysis device 3 for testing through the first interface 211 (S interface) of the microfluidic switching valve 2. Other samples flowing into interfaces 1, 3, 4, and 5 are discharged into the waste liquid collection device 6 through the third interface 213 (W interface).

[0082] After the sample from port 2 has been tested, the microfluidic switching valve 2 is rotated counterclockwise by 120° again, connecting the first port 211 (S port) and the third port 213 (W port) of the rotated microfluidic switching valve 2 to clean the pipeline. The samples corresponding to ports 3, 4, and 5 are then tested and cleaned using the same steps.

[0083] In this way, when detecting samples flowing into port 1, the microfluidic switching valve 2 only needs two ports—port 1 and the first port 211 (S port)—to receive the sample flowing out of the sample introduction device 1 and deliver it to the analytical device 3. Similarly, when detecting samples flowing into port 2, the microfluidic switching valve 2 only needs two ports—port 2 and the first port 211 (S port)—to receive the sample flowing out of the sample introduction device 1 and deliver it to the analytical device 3. This simplifies the structure of the microfluidic switching valve 2, simplifies the pipeline layout, and reduces the manufacturing difficulty and production cost of the microfluidic switching valve 2.

[0084] In a preferred embodiment of the present invention, such as Figure 6 As shown, the multi-sample injection analysis system has a cleaning flow path, which sequentially connects the cleaning fluid supply device 4, the second switching valve 5, the third interface 213 (W interface shown), the first channel 214, the first interface 211 (S interface shown), and the analysis device 3. The cleaning fluid is delivered to the inlet of the analysis device 3 through the second switching valve 5 via the third interface 213, the first channel 214, and the first interface 211, thereby cleaning the pipeline from the inlet of the analysis device 3 to the microfluidic switching valve 2. This cleaning flow path structure is simple in structure, has few flow channels, and simple in wiring layout, reducing the manufacturing difficulty and production cost of the microfluidic switching valve 2.

[0085] In a preferred embodiment of the present invention, the multi-sample injection analysis system further includes a waste liquid collection device 6, and the second switching valve 5 further includes a valve body opening connected to the waste liquid collection device 6. The second switching valve 5 can switch the third interface 213 to the cleaning fluid supply device 4 or the waste liquid collection device 6.

[0086] In this manner, when a sample is injected through one second port 212, the sample flowing into the other second ports 212 enters the waste liquid collection device 6 through the third port 213, thereby realizing waste liquid recovery and reducing environmental pollution. The second switching valve 5 has a simple structure and can be implemented using a three-way valve, which helps to reduce production costs and enables switching between waste liquid recovery and pipeline cleaning, making it highly practical.

[0087] In a preferred embodiment of the present invention, the analytical device 3 is a liquid chromatography apparatus. In some embodiments, the analytical device 3 may not be a liquid chromatography apparatus, but may be other analytical devices adapted to multi-sample injection and cleaning systems. The embodiments of the present invention can be used for sample injection and cleaning of liquid chromatography apparatuses, improving the cleanliness of liquid chromatography sample injection, reducing cross-contamination, and improving the testing accuracy of liquid chromatography apparatuses.

[0088] In a preferred embodiment of the present invention, the sample introduction device 1 includes a microfluidic injection pump 11 and a microfluidic chip mounting box 12. The microfluidic chip mounting box 12 houses a microfluidic chip 121, which has multiple sample channels 122. The inlet of each sample channel 122 is connected to the outlet of the microfluidic injection pump 11, and the outlet of each sample channel 122 is connected to a second interface 212. When the microfluidic injection pump 11 introduces samples, each introduction unit maintains a slow injection rate, thereby ensuring that the cells in each sample channel 122 of the microfluidic chip 121 can continue to survive and remain stably within the microfluidic chip 121. It should be noted that although this embodiment is described based on an automated sample introduction analysis process for cell analysis experiments, the microfluidic switching valve and multi-sample introduction analysis system provided by the present invention are not limited to this application and can also be applied in other scenarios where a microfluidic chip requiring constant microfluidic flow is needed.

[0089] In a preferred embodiment of the present invention, the cleaning fluid supply device 4 may include a cleaning fluid supply pump and a cleaning fluid storage device. The cleaning fluid is introduced by pumping, which is simple, easy to implement, and helps to save costs.

[0090] In the above embodiments of the present invention, an example of having one first interface 211 and five second interfaces 212 has been described. However, the number of second interfaces 212 is not limited to five. For example, 11 second interfaces 212 can be set. In this case, 11 samples to be tested can be tested one by one.

[0091] Second Implementation Method

[0092] The second embodiment of the present invention provides a more preferred multi-sample injection analysis system and a multi-sample injection analysis method for the multi-sample injection analysis system. Compared with the multi-sample injection analysis system in the first embodiment, the multi-sample injection analysis system and the multi-sample injection analysis method for the multi-sample injection analysis system in the second embodiment of the present invention can simplify the pipeline and clean the sample after injection, and can also accumulate the sample before injection, thereby reducing the time for multi-sample injection and detection.

[0093] Figure 7 This is a schematic diagram of the structure of the multi-sample injection analysis system provided in the second embodiment of the present invention, as shown below. Figure 7 As shown, the multi-sample injection analysis system includes a microfluidic switching valve 2, an injection device 1, multiple sample loops 7 (sample loops #1-#5), an analytical device 3, a buffer supply device 8, and a buffer flow path switching device 9. The microfluidic switching valve 2, the injection device 1, and the analytical device 3 have been described in detail in the first embodiment and will not be repeated here.

[0094] Compared to the multi-sample injection analysis system in the first embodiment, the multi-sample injection analysis system in this embodiment also has multiple sample loops 7 correspondingly set on multiple injection flow paths 100 between the injection device 1 and the microfluidic switching valve 2, and adds a buffer supply device 8 connected to the inlet of the multiple sample loops 7 and a buffer flow path switching device 9 for controlling the connection mode of the inlet of the multiple sample loops 7.

[0095] Each sample loop 7 is positioned on a corresponding sample inlet flow path 100. The inlet of the sample loop 7 is connected to the buffer flow path switching device 9, and the outlet of the sample loop 7 is connected to the second interface 212 (interface shown in Figures 1-5) of the microfluidic switching valve 2. The sample loop 7 can be any container with a quantitative liquid storage capacity, including but not limited to... Figure 7 The fixed-length annular tubing shown can also be, for example, a quantitative tube with a fixed volume. The buffer flow path switching device 9 can control the inlet of the sample loop 7 to be connected to the buffer supply device 8 or the injection device 1. Before detection, the inlets of multiple sample loops 7 can be switched to be connected to the sample outlet of the injection device 1. The injection device 1 simultaneously injects samples into multiple sample loops 7 until a sufficient amount of sample is accumulated in multiple sample loops 7 (generally, the sample loops 7 are filled). This completes the sample pre-accumulation process in multiple injection flow paths 100 simultaneously. Therefore, in subsequent multi-sample detection, the sample in the sample loop 7 can be directly sent to the analysis device 3 for detection, saving injection time.

[0096] The buffer supply device 8 has multiple buffer supply flow paths corresponding to multiple sample loops 7, used to supply buffer to the inlets of multiple sample loops 7. In some embodiments, the buffer supply device 8 may include multiple buffer inlets and multiple independent buffer supply flow paths corresponding to the multiple buffer inlets, thereby realizing the supply to multiple sample loops 7. In some preferred embodiments, the buffer supply device 8 may also be a switching valve with one buffer inlet and multiple buffer outlets, and the supply to multiple sample loops 7 is realized by switching the connection between the interfaces.

[0097] The buffer solution flow path switching device 9 can be one or more switching valves, used to control the switching of each flow path as a whole or individually. Preferably, the buffer solution flow path switching device 9 is a plurality of third switching valves (third switching valves 91, 92, 93, 94, and 95 in the figure), and the plurality of third switching valves are respectively arranged on each sample injection flow path 100. Therefore, the sample loop 7 in each sample injection flow path 100 can be individually controlled by the plurality of third switching valves to connect to the sample injection device 1 or the buffer solution supply device 8. Each third switching valve has three interfaces: a first interface (top of the figure) connected to the inlet of sample loop 7, a second interface (bottom right of the figure) connected to the sample outlet of injection device 1, and a third interface (bottom left of the figure) connected to buffer supply device 8. The first interface can be connected to either the second or the third interface. When the first interface is connected to the second interface, the inlet of sample loop 7 is switched to be connected to the sample outlet of injection device 1; when the first interface is connected to the third interface, the inlet of sample loop 7 is switched to be connected to buffer supply device 8.

[0098] For example, such as Figure 7 As shown, before detection, the first and second ports of all the third switching valves (91, 92, 93, 94, and 95 in the figure) are connected. At this time, the inlets of sample loops #1-#5 are switched to be connected to the sample outlet of the injection device 1. The injection device 1 continuously injects sample into sample loops #1-#5 until they are full, thus completing the sample pre-accumulation process. Then, when detecting sample loop #1, it is only necessary to switch the third switching valve 91 corresponding to the inlet of sample loop #1 to be connected to the first and third ports. At this time, the inlet of sample loop #1 is switched to be connected to the buffer outlet of the buffer supply device 8. The buffer supply device 8 continuously supplies buffer to the inlet of sample loop #1, and the sample in sample loop #1 is pushed into the analysis device 3 for analysis by the buffer. Therefore, the sample ring 7 enables simultaneous sample storage on multiple flow paths, shortening the multi-sample detection time and allowing continuous sample injection in the multi-sample injection analysis system, thus improving detection accuracy. Furthermore, the sample ring 7 is installed in multiple injection flow paths 100, allowing for simultaneous or independent sample storage. This also allows for sample accumulation in other flow paths while sample detection is being performed in one path. Additionally, sample accumulation and injection cleaning operations can be achieved through simple valve settings and flow path switching, significantly simplifying the device.

[0099] Preferably, such as Figure 8As shown, the buffer supply device 8 may include a fourth switching valve 81, which has a fourth interface 811 located at the center of the fourth switching valve 81; and a plurality of fifth interfaces 812 (i.e., Figure 8 The buffer supply device 8 has interfaces 1-5 arranged around the fourth interface 811 in the fourth switching valve 81; the third channel 814 has one end connected to the fourth interface 811 and the other end can be selectively connected to any one of the multiple fifth interfaces 812, and is not connected to the other multiple fifth interfaces 812; the multiple fifth interfaces 812 of the fourth switching valve 81 are respectively connected to the multiple third switching valves; and the fourth interface 811 is connected to the buffer inlet.

[0100] More preferably, the buffer supply device 8 further includes a buffer injection pump 82 disposed at the buffer inlet end, the outlet of the buffer injection pump 82 being connected to the fourth interface 811 for driving the buffer flowing in from the buffer inlet to flow in the system.

[0101] Specifically, the buffer solution is driven by the buffer injection pump 82 and flows into the buffer supply device 8 through the fourth port 811 of the fourth switching valve 81. The fifth port 812 surrounds the fourth port 811. The third channel 814 formed by the connection between the fourth port 811 and the fifth port 812 is the buffer supply path. When the buffer supply device 8 is connected to the sample loop 7, the fourth port 811 is connected to the fifth port 812 corresponding to that sample loop 7, forming the buffer supply path. The buffer solution flows to the sample loop 7 through the buffer supply path, pushing the samples in different sample loops 7 into the analysis device 3. The control and switching of the flow of buffer solution into different sample loops 7 can be realized with a single valve, simplifying the device structure.

[0102] The buffer solution needs to enter the system through the buffer solution inlet, and then flow sequentially through the fourth interface 811, the fifth interface 812, the sample loop, the second interface 212, the first interface 211, and the analytical device 3. The buffer solution needs to flow continuously during the sample injection and cleaning processes. In order to ensure the continuous flow of the buffer solution in the system, a large driving force needs to be applied to the buffer solution. Preferably, the buffer solution injection pump 82 can be a high-pressure injection pump to achieve buffer solution driving in a long flow path.

[0103] The multi-sample injection analysis system provided by the second embodiment of the present invention can not only achieve switching of multi-sample detection with a small number of interfaces and low manufacturing cost, but also can simultaneously carry out the sample accumulation process through sample rings 7 on multiple injection flow paths 100, further reducing multi-sample detection time and sample waste.

[0104] The second embodiment of the present invention also provides a multi-sample injection analysis method for use in the multi-sample injection analysis system as described in any of the above embodiments, such as... Figure 9As shown, this multi-sample injection analysis method includes the following steps:

[0105] In the liquid storage step S1, the sample enters through multiple sample outlets and fills multiple sample loops;

[0106] In sample introduction step S2, the buffer supply device is switched one by one to the inlet of different sample loops, and the buffer solution is used to push the sample in each sample loop into the analysis device.

[0107] In detection step S3, the analytical equipment performs detection and analysis on the sample.

[0108] Preferably, in the sample injection step, after the sample loop is injected, a cleaning step S4 is also performed, in which buffer solution is continuously introduced into the inlet of the sample loop to clean the injection and detection tubing.

[0109] Preferably, if the plurality of sample rings includes at least one unfilled sample ring and at least one filled sample ring, the liquid storage step for the unfilled sample ring and the sample injection step for the filled sample ring are performed simultaneously.

[0110] The following example illustrates the multi-sample injection analysis method using the multi-sample injection analysis system provided in this embodiment.

[0111] Before use, perform the liquid storage step S1, such as... Figure 8 As shown, the first interface of the third switching valve (the third switching valves 91, 92, 93, 94, and 95 shown in the figure) is connected to the second interface of the third switching valve. At this time, the inlets of sample rings #1-#5 are all switched to be connected to the sample outlet of the injection device 1. The injection device 1 continuously injects samples into sample rings #1-#5 until sample rings #1-#5 are filled, thus completing the liquid storage step S1 of sample rings #1-#5.

[0112] Next, proceed with injection step S2. Figure 10 The diagram shown illustrates the multi-sample injection analysis system provided in this embodiment detecting sample loop #1. Figure 10 As shown, the third switching valve 91 corresponding to the inlet of sample loop #1 is switched to connect the first interface of the third switching valve with the third interface of the third switching valve. Furthermore, the second interface 212 (interface 1) of the microfluidic switching valve 2 corresponding to the outlet of sample loop #1 is switched to connect with the first interface 211 (interface S). At this time, the inlet of sample loop #1 is switched to connect with the outlet of the buffer injection pump 82 of the buffer supply device 8, and the outlet of sample loop #1 is connected to the analysis device 3 through the first channel 214 of the microfluidic switching valve 2. The buffer injection pump 82 drives the buffer to flow into sample loop #1 and pushes the sample in sample loop #1 into the analysis device 3.

[0113] Then, detection step S3 is performed, and the analysis device 3 performs detection and analysis on the sample in sample ring #1.

[0114] After the detection step S3 is completed, the cleaning step S4 is performed. For a certain period of time after the detection is completed, the buffer injection pump 82 continuously introduces buffer solution into the inlet of the sample loop #1. The buffer solution cleans the sample injection and detection pipeline, and finally flows into the waste liquid collection device 6 through the detection pipeline.

[0115] After completing the detection of one sample ring 7, the injection step S2, detection step S3 and cleaning step S4 are performed again on different sample rings 7 (full sample rings). At the same time, the liquid storage step S1 is performed on the sample rings 7 that have completed the detection (not full sample rings). Figure 11 The diagram shown illustrates the multi-sample injection analysis system provided in this embodiment detecting sample loop #2. Figure 11 As shown, the liquid storage step S1 is performed on the flow path corresponding to sample ring #1. The third switching valve 91 corresponding to the inlet of sample ring #1 is switched to connect the first interface of the third switching valve and the second interface of the third switching valve. The injection device 1 is connected to the inlet of sample ring #1 to store liquid in sample ring #1. At the same time, the injection step S2 is performed on the flow path corresponding to sample ring #2. The third switching valve 92 corresponding to the inlet of sample ring #2 is switched to connect the first interface of the third switching valve and the third interface of the third switching valve. Furthermore, the first valve body 21 of the microfluidic switching valve 2 is kept stationary, and the second valve body 22 is rotated 60° clockwise, so that the second interface 212 of the microfluidic switching valve 2 corresponding to the outlet of sample ring #2 ( Figure 11 The No. 2 interface is switched to connect with the first interface 211 (S interface). At this time, the inlet of sample loop #2 is switched to connect with the outlet of the buffer injection pump 82 of the buffer supply device 8, and the outlet of sample loop #2 is connected to the analysis device 3 through the first channel 214 of the microfluidic switching valve 2. The sample in sample loop #2 is pushed into the analysis device 3 by the buffer solution. Then, the detection step S3 and the cleaning step S4 are executed to realize the detection of the sample in sample loop #2.

[0116] In this second embodiment, by using the above methods, the sample rings 7 on multiple sample flow paths 100 are used to simultaneously perform the sample injection step S1, and the sample injection step S1 for the incomplete sample rings 7 and the liquid storage step S2 for the completed sample rings are performed simultaneously, which can significantly save the time for multi-sample detection. Furthermore, by using the buffer solution to push the sample in the sample ring into the analytical device, the tubing can be directly cleaned with the buffer solution after sample injection without any other switching operations, simplifying the steps for multi-sample detection.

[0117] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A microfluidic switching valve for a multi-sample injection analysis system, comprising: First valve body; Second valve body; The first interface is located at the center of the first valve body; Multiple second interfaces are arranged around the first interface in the first valve body; A third interface, in the same form as the second interface, is provided in the first valve body; The first channel has one end connected to the first interface and the other end selectively connected to any one of the plurality of second interfaces or the third interface, and is not connected to the other plurality of second interfaces or the third interface. The first channel is disposed in the second valve body. Its characteristic is that it further includes a second channel, the second channel being disposed in the second valve body. The plurality of second interfaces and the third interface that are not connected to the first channel are connected to each other through the second channel; or, the plurality of second interfaces that are not connected to the first channel are connected to each other through the second channel.

2. The microfluidic switching valve for a multi-sample injection analysis system according to claim 1, characterized in that, The plurality of second interfaces are arranged in a ring-shaped and equally spaced manner on the cross-section of the first valve body; The third interface is arranged in the same ring as the second interface and at the same distance from the second interface.

3. The microfluidic switching valve for a multi-sample injection analysis system according to claim 1 or 2, characterized in that, The first interface is connected to the inlet of the analysis device, and the plurality of second interfaces are connected to the outlet of the sample injection device.

4. The microfluidic switching valve for a multi-sample injection analysis system according to claim 3, characterized in that, The third interface is connected to the cleaning fluid supply device and / or the waste fluid collection device.

5. A multi-sample injection analysis system, comprising the microfluidic switching valve according to any one of claims 1-4, characterized in that, It also includes a sample introduction device, analytical equipment, and a cleaning solution supply device, among which, The first interface is connected to the inlet of the analysis device; The sample introduction device has multiple sample outlets, and each sample outlet is connected to a corresponding second interface. The third interface is connected to the cleaning fluid supply device.

6. The multi-sample injection analysis system according to claim 5, characterized in that, It also includes a second switching valve, which is disposed on the pipeline between the outlet of the cleaning fluid supply device and the inlet of the third interface.

7. The multi-sample injection analysis system according to claim 6, characterized in that, It also includes a waste liquid collection device, and the second switching valve further includes a valve body opening that communicates with the waste liquid collection device. The second switching valve can switch the third interface to the cleaning fluid supply device or the waste liquid collection device.

8. The multi-sample injection analysis system according to any one of claims 5-7, characterized in that, The analytical equipment is a liquid chromatography apparatus.

9. The multi-sample injection analysis system according to claim 8, characterized in that, The sample introduction device includes a microfluidic injection pump and a microfluidic chip. The microfluidic chip has multiple sample channels. The inlet of each sample channel is connected to the outlet of the microfluidic injection pump, and the outlet of each sample channel is connected to a corresponding second interface.

10. The multi-sample injection analysis system according to claim 9, characterized in that, It also includes a microfluidic chip mounting box, in which the microfluidic chip is disposed.

11. A multi-sample injection analysis system, comprising the microfluidic switching valve according to any one of claims 1-4, characterized in that, It also includes a sample introduction device, multiple sample loops, analytical equipment, a buffer supply device, and a buffer flow path switching device, among which, The sample introduction device has multiple sample outlets, each of which is connected to a second interface via a sample ring; The inlet of the analysis device is connected to the first interface; The buffer solution flow path switching device is used to switch the buffer solution supply device to the inlet of different sample loops.

12. The multi-sample injection analysis system according to claim 11, characterized in that, The buffer flow path switching device includes multiple third switching valves, which are respectively disposed between the sample outlet and the inlet of the sample loop, for independently switching the inlet of each sample loop to the sample outlet or to the buffer supply device.

13. The multi-sample injection analysis system according to claim 12, characterized in that, The buffer supply device includes a fourth switching valve, the fourth switching valve having, The fourth interface is located at the center of the fourth switching valve; Multiple fifth interfaces are arranged around the fourth interface in the fourth switching valve; The third channel has one end connected to the fourth interface, and the other end can be selectively connected to any one of the plurality of fifth interfaces, but is not connected to the other plurality of fifth interfaces. The fourth switching valve has multiple fifth ports that are respectively connected to the multiple third switching valves, and the fourth port is connected to the buffer inlet.

14. The multi-sample injection analysis system according to claim 11, characterized in that, The sample injection device also includes a microfluidic injection pump and a microfluidic chip. The microfluidic chip has multiple sample channels, and the inlet of each sample channel is connected to the outlet of the microfluidic injection pump.

15. A multi-sample injection analysis method, used in the multi-sample injection analysis system as described in any one of claims 11-14, characterized in that, Includes the following steps: In the liquid storage step, the sample enters through the multiple sample outlets and fills the multiple sample loops; In the sample introduction step, the buffer supply device is switched one by one to the inlet of different sample loops, and the buffer solution is used to push the sample in each sample loop into the analysis device. The detection step involves the analytical equipment performing detection and analysis on the sample.

16. The multi-sample injection analysis method as described in claim 15, characterized in that, In the injection step, after the injection of each sample loop is completed, the following is also performed: During the cleaning step, buffer solution is continuously introduced into the inlet of the sample loop to clean the sample injection and detection tubing.

17. The multi-sample injection analysis method according to claim 15, characterized in that, If the plurality of sample rings includes at least one unfilled sample ring and at least one filled sample ring, the liquid storage step for the unfilled sample ring and the sample injection step for the filled sample ring are performed simultaneously.

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