A kit for detecting AChR blocking antibodies, a detection method and application

By using compounds such as acetylcholine bromide or carbacholine in combination with the CBA method to replace α-BGT for detecting AChR blocking antibodies, the problems of toxicity and radiolabeling are solved, and a safe, low-cost and efficient detection method is achieved.

CN116773803BActive Publication Date: 2026-06-16SHAANXI MYBIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHAANXI MYBIOTECH CO LTD
Filing Date
2022-10-19
Publication Date
2026-06-16

AI Technical Summary

⚠Technical Problem

In existing technologies, methods for detecting AChR blocking antibodies use α-Bora tamarind venom (α-BGT), which is toxic, difficult to obtain, and requires radioactive labeling, resulting in time-consuming experimental processes and significant harm to the environment and personnel.

⚗Method used

By using compounds such as brominated acetylcholine or carbacholine to replace α-BGT and combining them with the CBA method for detection, and by competitively binding to the AChR binding site, a non-radioactive fluorescent labeling method is used to shorten the detection time and reduce costs.

🎯Benefits of technology

It enables safe, low-cost, and rapid detection of AChR blocking antibodies, avoiding the hazards of radioactive materials and environmental impact, and improving detection efficiency.

✦ Generated by Eureka AI based on patent content.

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Abstract

The application provides a kit for detecting AChR blocking antibodies, a detection method and application, and belongs to the technical field of medical detection. The application provides a compound for detecting AChR blocking antibodies instead of alpha-BGT. Compared with alpha-BGT, the compound is non-toxic, cheap, easy to obtain, and can still detect AChR blocking antibodies after high-temperature treatment. The application also provides a method for detecting AChR blocking antibodies by using the compound. The compound does not need to be labeled with radioactive iodine or other substances, thereby reducing the cost, avoiding the influence of radioactive substances on the environment and the harm to experimenters, and being green and environmentally friendly. The compound can also be incubated with a serum sample at the same time, thereby shortening the detection time and improving the detection efficiency.
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