A composition for improving nitrogen absorption in wheat plants

Abstract

The present invention belongs to the technical field of pesticides, and specifically relates to a composition for improving nitrogen absorption in wheat plants. A composition for improving nitrogen absorption in wheat plants, the active ingredient of which is composed of a binary compound of pyraclostrobin and cyclodipeptide or reserpine. The composition for improving nitrogen absorption in wheat plants of the present invention screens pyraclostrobin for compounding with other types of active ingredients. Among them, pyraclostrobin can improve the nitrogen absorption of plants, promote the rapid growth of crops, and increase crop yields, thereby achieving the purpose of high crop yields; and the entire compounding formula has a synergistic effect on fungal diseases such as wheat gray mold within a certain mass ratio range, can improve the control effect on wheat diseases, and further can reduce the application amount of pesticides, reduce the control cost and pesticide residues. In addition, it can delay the generation of disease resistance, reduce the resistance risk of using a single active ingredient, and is safe and efficient.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a composition for improving nitrogen absorption of wheat plants. Background Art

[0002] Pyraclostrobin is a strobilurin fungicide, which is a mitochondrial respiration inhibitor. It inhibits mitochondrial respiration by blocking the electron transfer between cytochrome b and c1, so that mitochondria cannot produce and provide the energy ATP required for normal cell metabolism, ultimately leading to cell death. When controlling diseases of cereal crops, due to its broad-spectrum fungicidal activity, pyraclostrobin has outstanding control effects on leaf and spike diseases of cereals, and the yield increase effect is remarkable. In addition, in addition to having a direct effect on pathogenic bacteria, pyraclostrobin can also induce physiological phenomena in many crops, especially cereals, such as improving the nitrogen absorption of plants, thereby promoting the rapid growth of crops and increasing crop yields, so as to achieve the purpose of high crop yields. As can be seen from the above, pyraclostrobin can not only control fungal diseases of crops, but also improve plant nitrogen absorption. Although pyraclostrobin has the above advantages, like many existing chemical agents, it has a problem: long-term single use easily makes pathogenic bacteria produce drug resistance, thereby reducing the control effect on pathogenic bacteria. Therefore, it is necessary to develop a compound or mixture of pyraclostrobin.

[0003] Cyclic dipeptides are a class of simple peptide compounds with a stable six-membered ring structure formed by cyclization of two amino acids through peptide bonds. Cyclo(L-proline-L-valine) dipeptide, CAS: 2854-40-2, molecular formula: C 10 H 16 N2O2, molecular weight: 196.25, and the structural formula is as follows:

[0004]

[0005] Application No. CN201810027375.5 discloses the application of cyclo(L-proline-L-valine) dipeptide in the preparation of fungicides, and specifically discloses that cyclo(L-proline-L-valine) dipeptide can inhibit the growth of pathogenic bacteria such as Botrytis cinerea, Ascochyta citrullina, Magnaporthe oryzae, Fusarium moniliforme, Fusarium oxysporum and Rhizoctonia solani, providing a new idea for the research and development of fungicides.

[0006] Reserpine, CAS: 50-55-5, molecular formula: C 33 H 40 N2O9, and the structural formula is as follows:

[0007]

[0008] CN201810979216.5 discloses the application of reserpine as a bactericidal synergist in the control of Botrytis cinerea in plants. At the same time, it also discloses that reserpine has a synergistic effect when compounded with various agents such as boscalid, azoxystrobin, carbendazim or difenoconazole.

[0009] At present, there is no relevant report on the compounding of pyraclostrobin with cyclodipeptide or reserpine.

[0010] The information disclosed in this background art section is only intended to increase the understanding of the overall background of the present invention, and should not be regarded as an admission or any form of implication that this information constitutes the prior art already known to those of ordinary skill in the art. Summary of the Invention

[0011] The purpose of the present invention is to provide a composition for improving nitrogen absorption in wheat plants, so as to solve the problem that long-term single use of pyraclostrobin easily causes pathogenic bacteria to develop drug resistance, thereby reducing the control effect on pathogenic bacteria.

[0012] To achieve the above purpose, the present invention provides the following technical solutions:

[0013] A composition for improving nitrogen absorption in wheat plants, the active ingredient of which is composed of a binary compound of pyraclostrobin and cyclodipeptide or reserpine.

[0014] Preferably, the mass ratio of pyraclostrobin to cyclodipeptide is 1-27:6-1.

[0015] Preferably, the mass ratio of pyraclostrobin to reserpine is 1-18:2-1.

[0016] The present invention also provides the application of the composition for improving nitrogen absorption in wheat plants in the control of wheat fungal diseases.

[0017] Preferably, the wheat fungal diseases include wheat gray mold, wheat scab and wheat powdery mildew.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) The composition for improving nitrogen absorption in wheat plants of the present invention screens pyraclostrobin for compounding with other types of active ingredients. Among them, pyraclostrobin can improve the nitrogen absorption of plants, promote the rapid growth of crops, and increase crop yields, so as to achieve the purpose of high crop yields; and the entire compounding formula has a synergistic effect on fungal diseases such as wheat gray mold within a certain mass ratio range, which can improve the control effect on wheat diseases, and further can reduce the application amount of pesticides, reduce the control cost and pesticide residues.

[0020] (2) The composition of the present invention for improving nitrogen absorption in wheat plants can delay the generation of disease drug resistance, reduce the drug resistance risk of using a single active ingredient, and is safe and efficient. Detailed implementation mode

[0021] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work belong to the scope protected by the present invention.

[0022] Example : Screening of control agents

[0023] 1. Test strains: Botrytis cinerea Pers ex Fr. on wheat plants collected from the leaves of diseased wheat plants in the wheat planting area of Zhengzhou, Henan, were isolated, purified and stored on PDA medium in the laboratory.

[0024] 2. Test agents

[0025] 97.5% pyraclostrobin (Hunan Haili Chemical Industry Co., Ltd.), 98% cyclo - dipeptide (Zhengzhou Huiju Chemical Co., Ltd.), 98% reserpine (Zhengzhou Huiju Chemical Co., Ltd.)

[0026] After dissolving the test agents, they were diluted with 0.1% Tween - 80 aqueous solution to prepare single - agent mother liquors. Multiple groups of ratios were set, and 5 mass concentration gradients were set for each single - agent mother liquor and the mixed agents of ratios according to the equal - ratio method.

[0027] 3. Test method (refer to "NY / T 1156.2 - 2006 Pesticide indoor bioassay test guidelines Fungicides Part 2: Inhibiting the mycelial growth of pathogenic fungi Petri dish method")

[0028] Under sterile conditions, the pre - melted PDA medium was added to a sterile conical flask. 5 mL of the liquid medicine was quantitatively pipetted in sequence from low concentration to high concentration and added to the above - mentioned conical flasks containing 45 mL of medium respectively. After shaking well, it was poured into a Petri dish with a diameter of 9 cm. 10 mL of the medicated medium was poured into each Petri dish to make corresponding concentration medicated plates. Each concentration had 3 replicates, and a treatment without the agent was set as a blank control.

[0029] Under sterile conditions, cut out fungal disks with a diameter of 5 mm from the edge of the colony of the cultivated pathogenic bacteria using a sterilized borer. Inoculate the fungal disks in the center of the drug-containing petri dish with an inoculator, with the mycelial surface facing upwards. Cover the petri dish lid and place it in an incubator at 25 °C for cultivation. When the diameter of the blank control colony grows to 2 / 3 of the diameter of the petri dish, measure the colony diameter with a caliper. Measure the diameter of each colony vertically once using the cross method, take the average value, and calculate the inhibition rate of mycelial growth for different treatments.

[0030]

[0031] Colony growth diameter = Colony diameter - Fungal disk diameter

[0032] 4. Data analysis: Use DPS software for data statistical analysis. Perform linear regression with the logarithm value of the fungicide concentration as x and the probit value of the corresponding mycelial growth inhibition rate as y to obtain the virulence regression equation and the virulence EC 50 value of the agent against the target pathogen, and calculate the co-toxicity coefficient (CTC) according to the Sun Yunpei method.

[0033]

[0034] In the above formula: ATI - Measured virulence index of the mixture; S - EC 50 , in mg / L; M - EC 50 , in mg / L.

[0035] TTI = TI A ×P A +TI B ×P B

[0036] In the above formula: TTI - Theoretical toxicological index of the mixture; TI A - Virulence index of agent A; P A - Percentage content of agent A in the mixture, in percentage (%); TI B - Virulence index of agent B; P B - Percentage content of agent B in the mixture, in percentage (%).

[0037]

[0038] In the above formula: CTC - Co-toxicity coefficient; ATI - Measured virulence index of the mixture; TTI - Theoretical virulence index of the mixture.

[0039] 5. Measurement results

[0040] The synergistic effect of the agents was evaluated based on the calculated co-toxicity coefficient (CTC). When CTC ≤ 80, it indicates an antagonistic effect; when 80 < CTC < 120, it indicates an additive effect; when CTC ≥ 120, it indicates a synergistic effect. The results are shown in Table 1-2.

[0041] Table 1 Indoor biological activity of the compound of pyraclostrobin and cyclodipeptide against Botrytis cinerea of wheat

[0042] Name and ratio of the medicament EC50 (mg / L) ATI TTI CTC Pyraclostrobin 1.3057 100.0000 -- -- Cyclic dipeptide 42.9874 3.0374 -- -- Pyraclostrobin 1: Cyclic dipeptide 6 2.6203 49.8302 16.8892 295.0416 Pyraclostrobin 1: Cyclic dipeptide 3 1.4106 92.5634 27.2781 339.3331 Pyraclostrobin 1: Cyclic dipeptide 1 2.0838 62.6596 51.5187 121.6249 Pyraclostrobin 3: Cyclic dipeptide 1 1.0132 128.8689 75.7594 170.1030 Pyraclostrobin 6: Cyclic dipeptide 1 1.1308 115.4669 86.1482 134.0329 Pyraclostrobin 18: Cyclic dipeptide 1 0.6975 187.1971 94.8967 197.2641 Pyraclostrobin 27: Cyclic dipeptide 1 0.3237 403.3673 96.5371 417.8368

[0043] Table 2 Indoor biological activity of the compound of pyraclostrobin and reserpine against Botrytis cinerea of wheat

[0044] Name and ratio of the medicament EC50 (mg / L) ATI TTI CTC Pyraclostrobin 1.3057 100.0000 -- -- Reserpine 12.0426 10.8423 -- -- Pyraclostrobin 1: Reserpine 2 1.5599 83.7041 40.5616 206.3631 Pyraclostrobin 1: Reserpine 1 0.8455 154.4293 55.4212 278.6468 Pyraclostrobin 3: Reserpine 1 1.3912 93.8542 77.7106 120.7741 Pyraclostrobin 6: Reserpine 1 0.4549 287.0301 87.2632 328.9246 Pyraclostrobin 18: Reserpine 1 0.7818 167.0120 95.3075 175.2349

[0045] As can be seen from Table 1, after the compounding of pyraclostrobin and cyclodipeptide, the co-toxicity coefficients against Botrytis cinerea of wheat were all greater than 120 in the mass ratio range of 1-27:6-1, showing a synergistic effect.

[0046] As can be seen from Table 2, after the compounding of pyraclostrobin and reserpine, the co-toxicity coefficients against Botrytis cinerea of wheat were all greater than 120 in the mass ratio range of 1-18:2-1, showing a synergistic effect.

[0047] In summary, the compounding of pyraclostrobin with cyclodipeptide or reserpine has a good synergistic effect on Botrytis cinerea of wheat, which can improve the control effect on Botrytis cinerea of wheat and reduce the resistance risk of single use of pyraclostrobin.

[0048] The foregoing description of the specific exemplary embodiments of the present invention is for the purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many changes and variations are possible in light of the above teaching. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical applications, so that those skilled in the art can implement and utilize the various different exemplary embodiments of the present invention, as well as various different selections and changes. The scope of the present invention is intended to be defined by the claims and their equivalents.

Claims

1. A composition for improving nitrogen absorption of wheat plants, characterized in that, Its active ingredient is composed of the compound of pyraclostrobin and cyclic dipeptide, wherein the mass ratio of pyraclostrobin to cyclic dipeptide is 1-27:6-1; the cyclic dipeptide is cyclo(L-proline-L-valine) dipeptide.

2. Use of the composition for improving nitrogen absorption of wheat plants according to claim 1 in the prevention and control of Botrytis cinerea of wheat.

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