Microbial preparation for conditioning water environment of aquaculture and preparation process thereof

Abstract

The present application relates to the field of aquaculture technology, and discloses a microbial preparation for conditioning water environment of aquaculture and a preparation process thereof. The present application prepares a microbial preparation with a capsule structure, which comprises the following raw materials: gelatin microspheres coated with mixed bacterial powder, sodium thiosulfate, ferric sulfate and nano titanium dioxide. When the microbial preparation is put into water, the gelatin is hydrolyzed, and the released sodium thiosulfate and ferric sulfate quickly decompose nitrite in the water through reduction. After a period of time, the gelatin shell of the gelatin microspheres is hydrolyzed, and the mixed bacterial powder is released. At this time, the sodium thiosulfate and ferric sulfate in the water have fully reacted, the content of nitrite in the water is low, and the water quality is good, thus providing a good environment for the initial growth of microorganisms and avoiding the problem that microorganisms will die in large quantities when directly put into water with a high content of nitrite. The nano titanium dioxide has a photocatalytic effect, and appropriate addition can help purify the water.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of aquaculture technology, and discloses a microbial preparation for conditioning the water environment of aquaculture and a preparation process thereof. BACKGROUND

[0002] In aquaculture, excessive nitrite often occurs. In the water body of aquaculture, the feed residues and excreta of cultured organisms that are not consumed often cannot be discharged in time, resulting in excessive nitrite content in the water body. High nitrogen content can lead to water quality deterioration, causing oxygen deficiency, poisoning and mass death of cultured organisms, and bringing huge economic losses to the breeders.

[0003] In the prior art, methods for treating nitrite include physical adsorption, chemical oxidation, chemical reduction and microbial degradation, but all have certain drawbacks: physical adsorption requires a large amount of adsorption material, chemical oxidation can harm the health of cultured organisms in the water body, chemical reduction has a quick effect but a short maintenance time and is prone to rebound, and the microorganisms used in microbial degradation often die in large quantities due to environmental inadaptability after entering the water body, etc.

[0004] Therefore, it is of great significance to prepare a microbial preparation for conditioning the water environment of aquaculture, which does not harm the health of cultured organisms, has a simple use method, a quick effect and a long-lasting effect. SUMMARY

[0005] The present application aims to provide a microbial preparation for conditioning the water environment of aquaculture and a preparation process thereof, to solve the problems presented in the background.

[0006] To solve the above technical problems, the present application provides the following technical solutions:

[0007] A preparation process of a microbial preparation for conditioning the water environment of aquaculture, comprising the following steps:

[0008] S1: Take gelatin and add it to water, stir and dissolve, add mixed bacterial powder, stir, ultrasonic dispersion, wash, filter, and obtain gelatin microspheres coated with bacterial powder;

[0009] S2: Fill sodium thiosulfate, ferric sulfate, nano titanium dioxide and gelatin microspheres into a hollow capsule prepared from gelatin, and obtain the microbial preparation.

[0010] More preferably, the microbial preparation comprises the following raw materials in terms of mass percentage: 18-25% sodium thiosulfate, 23-28% ferric sulfate, 3-6% nano titanium dioxide, and 45-55% gelatin microspheres.

[0011] More preferably, the mixed bacteria powder comprises the following raw materials in percentage by mass: 26-35% photosynthetic bacteria powder, 15-25% yeast powder, 25-35% nitrifying bacteria powder, 18-25% bacillus subtilis powder.

[0012] More preferably, the gelatin is water-soluble gelatin.

[0013] More preferably, the preparation of the photosynthetic bacteria powder comprises the following steps: inoculating activated photosynthetic bacteria seed liquid into solid culture medium, with light intensity of 3000-4000Lx, culturing for 45-50h, centrifuging, and drying to obtain the photosynthetic bacteria powder.

[0014] More preferably, the preparation of the yeast powder comprises the following steps: inoculating activated yeast seed liquid into solid culture medium, culturing for 30-40h under pressure of 0.02-0.05MP, centrifuging, and drying to obtain the yeast powder.

[0015] More preferably, the preparation of the nitrifying bacteria powder comprises the following steps: inoculating activated nitrifying bacteria seed liquid into solid culture medium, with fermentation aeration rate of 120-150%, culturing for 30-40h under pressure of 0.02-0.05MP, centrifuging, and drying to obtain the nitrifying bacteria powder.

[0016] More preferably, the preparation of the bacillus subtilis powder comprises the following steps: inoculating activated bacillus subtilis seed liquid into solid culture medium, with fermentation aeration rate of 120-150%, culturing for 30-40h under pressure of 0.02-0.05MP, centrifuging, and drying to obtain the bacillus subtilis powder.

[0017] More preferably, the preparation of the photosynthetic bacteria powder, the yeast powder, the nitrifying bacteria powder, and the bacillus subtilis powder comprises the following steps: inoculating photosynthetic bacteria, yeast, nitrifying bacteria, and bacillus subtilis into solid culture medium at 24-27℃ for 22-26h to obtain activated photosynthetic bacteria seed liquid, activated yeast seed liquid, activated nitrifying bacteria seed liquid, and activated bacillus subtilis seed liquid, respectively.

[0018] More preferably, the pH of the solid culture medium is 7-7.5; the solid culture medium comprises the following raw materials in concentration: 2.5-3.5g / L beef extract, 8-12g / L peptone, 3-6g / L sodium chloride, 12-17g / L agar, and the rest is water.

[0019] Compared with the prior art, the present application has the following beneficial effects:

[0020] (1) The microbial preparation prepared by the present application has a capsule structure, the capsule is made of water-soluble gelatin, after being put into water, the capsule shell is hydrolyzed to release sodium thiosulfate, ferric sulfate and nano titanium dioxide, the sodium thiosulfate and the ferric sulfate are reducing agents, which can quickly decompose nitrite in the water, so that the nitrite is reduced into gas and volatilized, at the same time, the sodium thiosulfate and the ferric sulfate can be used as a preventive agent for fish chronic nitrate poisoning, because of excessive nitrite, nitrite in the water reacts with hemoglobin in the organism to form cyanide methemoglobin, the sodium thiosulfate and the ferric sulfate can convert the cyanide ion into low-toxicity thiocyanate, which is then excreted by the organism, reducing the occurrence of poisoning events; the removal rate of nitrite in the water by the sodium thiosulfate and the ferric sulfate through the reduction method is greater than that by the microbial degradation method, so the microbial preparation can be used for emergency treatment of water bodies with excessively high nitrite content, the nitrite content in the water treated by the sodium thiosulfate and the ferric sulfate reduction method is reduced, the water quality is improved, and a good environment is provided for the initial growth of microorganisms in the subsequent process, avoiding the large amount of death of microorganisms due to environmental adaptation after entering the water body with excessively high nitrite content.

[0021] (2) The addition of nano titanium dioxide has a photocatalytic effect, and appropriate addition can help purify the water body.

[0022] (3) After the sodium thiosulfate and the ferric sulfate are released into the water body for a period of time, the gelatin shell of the gelatin microspheres wrapped with the mixed bacterial powder is also gradually hydrolyzed to release the mixed bacterial powder, at this time, the sodium thiosulfate and the ferric sulfate in the water body have fully played a reducing role, the nitrite content in the water body is low, and the water quality is good, providing a good environment for the initial growth of microorganisms, so that the microorganisms can grow and reproduce in large quantities, achieving the effect of long-term purification of nitrite in the water body, and the presence of the microorganisms solves the problems of short maintenance time and easy rebound of the sodium thiosulfate and the ferric sulfate in treating nitrite.

[0023] (4) The microbial preparation prepared by the present application only needs to be put into the water body, and the process of releasing sodium thiosulfate, ferric sulfate, nano titanium dioxide and then releasing mixed bacterial powder can be completed in turn through the gradual hydrolysis of gelatin, without the need for manual secondary placement, and the use is convenient; at the same time, due to the capsule structure of the microbial preparation, the microbial preparation is not easily washed away by the water flow after being put into the water body, and the problem of poor sedimentation performance of the microorganisms is also solved. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be described below in a clear and complete manner. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0025] The following raw materials are included in the following examples: beef extract (Beijing Aoboxing Biotechnology Co., Ltd.), proteose peptone (Beijing Aoboxing Biotechnology Co., Ltd.), sodium chloride (CAS: 7647-14-5), agar, sodium thiosulfate (CAS: 7772-98-7), ferric sulfate (CAS: 10028-22-5), gelatin (Shanghai Yuanye), empty capsules (model: Hongxing 00#, Shanghai Hongxing Capsule Co., Ltd.), nano titanium dioxide (~5 nm, Wuhan Haisan Technology Co., Ltd.), photosynthetic bacteria (GY-D100, Shanghai Guangyu Biotechnology Co., Ltd.), yeast (CICC-1028, Wuhan Ruicheng Biotechnology Co., Ltd.), nitrifying bacteria (GANDEW-NI, Gande brand), Bacillus subtilis (GY-D102, Shanghai Guangyu Biotechnology Co., Ltd.), flocculant (model: 3600DF, Ruili Water Purification);

[0026] The following percentages are mass percentages;

[0027] The following parts are mass parts;

[0028] Example 1: S1: Photosynthetic bacteria, yeast, nitrifying bacteria, and Bacillus subtilis were inoculated into solid culture medium at 25°C for activation, respectively, to obtain activated photosynthetic bacteria seed liquid, activated yeast seed liquid, activated nitrifying bacteria seed liquid, and activated Bacillus subtilis seed liquid. The pH of the solid culture medium was 7.4, and the activation time was 24 h. The solid culture medium included the following ingredients by concentration: beef extract 3 g / L, proteose peptone 10 g / L, sodium chloride 5 g / L, agar 15 g / L, and the rest was water;

[0029] S2: The activated photosynthetic bacteria seed liquid was inoculated into the solid culture medium, and the light intensity was maintained at 3500 Lx for 48 h of culture, centrifugation, and drying to obtain photosynthetic bacteria powder. The activated yeast seed liquid was inoculated into the solid culture medium and cultured under a pressure of 0.04 MP for 35 h, centrifuged, and dried to obtain yeast powder. The activated nitrifying bacteria seed liquid was inoculated into the solid culture medium, and the fermentation aeration rate was 130%, and the culture was carried out under a pressure of 0.045 MP for 36 h, centrifugation, and drying to obtain nitrifying bacteria powder. The activated Bacillus subtilis seed liquid was inoculated into the solid culture medium, and the fermentation aeration rate was 130%, and the culture was carried out under a pressure of 0.04 MP for 36 h, centrifugation, and drying to obtain Bacillus subtilis powder. The culture temperature was 28°C;

[0030] S3: 30% photosynthetic bacteria powder, 20% yeast powder, 30% nitrifying bacteria powder, and 20% Bacillus subtilis powder were mixed to obtain a mixed bacteria powder;

[0031] S4: Take 2 parts of gelatin into 100 parts of water, stir and dissolve at 70℃, pour into 60 parts of mixed bacteria powder, keep at 60℃, stir at 1290r / min for 0.5h, ultrasonic dispersion for 30min, wash, filter, and get gelatin microspheres coated with mixed bacteria powder;

[0032] S5: Fill 20% sodium thiosulfate, 25% ferric sulfate, 5% nano titanium dioxide, and 50% gelatin microspheres into gelatin hollow capsules to obtain a microbial preparation.

[0033] Example 2: S1: Photosynthetic bacteria, yeast, nitrifying bacteria, and Bacillus subtilis were inoculated into solid culture medium at 25℃ for activation, respectively, to obtain activated photosynthetic bacteria seed liquid, activated yeast seed liquid, activated nitrifying bacteria seed liquid, and activated Bacillus subtilis seed liquid; the pH of the solid culture medium was 7.4, and the activation time was 24h; the solid culture medium included the following components by concentration: beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L, and agar 15g / L, and the rest was water;

[0034] S2: The activated photosynthetic bacteria seed liquid was inoculated into the solid culture medium, and the light intensity was kept at 3500Lx for 48h of culture, centrifugation, and drying to obtain photosynthetic bacteria powder; the activated yeast seed liquid was inoculated into the solid culture medium, and cultured under 0.04MP pressure for 35h, centrifuged, and dried to obtain yeast powder; the activated nitrifying bacteria seed liquid was inoculated into the solid culture medium, and cultured under 0.045MP pressure for 36h with a fermentation aeration rate of 130%, centrifuged, and dried to obtain nitrifying bacteria powder; the activated Bacillus subtilis seed liquid was inoculated into the solid culture medium, and cultured under 0.04MP pressure for 36h with a fermentation aeration rate of 130%, centrifuged, and dried to obtain Bacillus subtilis powder; the culture temperature was 28℃;

[0035] S3: 26% photosynthetic bacteria powder, 15% yeast powder, 34% nitrifying bacteria powder, and 25% Bacillus subtilis powder were mixed to obtain mixed bacteria powder;

[0036] S4: Take 2 parts of gelatin into 100 parts of water, stir and dissolve at 70℃, pour into 60 parts of mixed bacteria powder, keep at 60℃, stir at 1290r / min for 0.5h, ultrasonic dispersion for 30min, wash, filter, and get gelatin microspheres coated with mixed bacteria powder;

[0037] S5: Fill 18% sodium thiosulfate, 23% ferric sulfate, 6% nano titanium dioxide, and 53% gelatin microspheres into gelatin hollow capsules to obtain a microbial preparation.

[0038] Example 3: S1: Photosynthetic bacteria, yeast, nitrifying bacteria, Bacillus subtilis were inoculated in solid culture medium at 25℃ for activation, respectively, to obtain activated photosynthetic bacteria seed liquid, activated yeast seed liquid, activated nitrifying bacteria seed liquid, and activated Bacillus subtilis seed liquid; the pH of the solid culture medium was 7.4, and the activation time was 24 h; the solid culture medium included the following components by concentration: beef extract 3 g / L, peptone 10 g / L, sodium chloride 5 g / L, agar 15 g / L, and the rest was water;

[0039] S2: The activated photosynthetic bacteria seed liquid was inoculated in the solid culture medium, the light intensity was maintained at 3500 Lx, and the culture was maintained for 48 h, followed by centrifugation and drying to obtain photosynthetic bacteria powder; the activated yeast seed liquid was inoculated in the solid culture medium, and the culture was maintained for 35 h under a pressure of 0.04 MP, followed by centrifugation and drying to obtain yeast powder; the activated nitrifying bacteria seed liquid was inoculated in the solid culture medium, the fermentation aeration rate was 130%, and the culture was maintained for 36 h under a pressure of 0.045 MP, followed by centrifugation and drying to obtain nitrifying bacteria powder; the activated Bacillus subtilis seed liquid was inoculated in the solid culture medium, the fermentation aeration rate was 130%, and the culture was maintained for 36 h under a pressure of 0.04 MP, followed by centrifugation and drying to obtain Bacillus subtilis powder; the culture temperature was 28℃;

[0040] S3: 35% photosynthetic bacteria powder, 22% yeast powder, 25% nitrifying bacteria powder, and 18% Bacillus subtilis powder were mixed to obtain mixed bacteria powder;

[0041] S4: Two parts of gelatin were added to 100 parts of water, stirred and dissolved at 70℃, poured into 60 parts of mixed bacteria powder, maintained at 60℃, stirred at a speed of 1290 r / min for 0.5 h, ultrasonically dispersed for 30 min, washed, filtered, and obtained gelatin microspheres coated with mixed bacteria powder;

[0042] S5: 25% sodium thiosulfate, 27% ferric sulfate, 3% nano titanium dioxide, and 45% gelatin microspheres were filled into gelatin hollow capsules to obtain a microbial preparation.

[0043] Comparative Example 1 (without using gelatin to coat the mixed bacteria powder, and the remaining steps were consistent with Example 1): S1: Photosynthetic bacteria, yeast, nitrifying bacteria, Bacillus subtilis were inoculated in solid culture medium at 25℃ for activation, respectively, to obtain activated photosynthetic bacteria seed liquid, activated yeast seed liquid, activated nitrifying bacteria seed liquid, and activated Bacillus subtilis seed liquid; the pH of the solid culture medium was 7.4, and the activation time was 24 h; the solid culture medium included the following components by concentration: beef extract 3 g / L, peptone 10 g / L, sodium chloride 5 g / L, agar 15 g / L, and the rest was water;

[0044] S2: inoculate the activated photosynthetic bacteria seed liquid into solid culture medium, keep the light intensity at 3500Lx, culture for 48h, centrifugal, dry, get photosynthetic bacteria powder; inoculate the activated yeast seed liquid into solid culture medium, culture for 35h under 0.04MP pressure, centrifugal, dry, get yeast powder; inoculate the activated nitrifying bacteria seed liquid into solid culture medium, fermentation aeration rate 130%, culture for 36h under 0.045MP pressure, centrifugal, dry, get nitrifying bacteria powder; inoculate the activated bacillus subtilis seed liquid into solid culture medium, fermentation aeration rate 130%, culture for 36h under 0.04MP pressure, centrifugal, dry, get bacillus subtilis powder; the culture temperature is 28℃;

[0045] S3: mix 30% photosynthetic bacteria powder, 20% yeast powder, 30% nitrifying bacteria powder, 20% bacillus subtilis powder, get mixed bacteria powder;

[0046] S4: fill 20% sodium thiosulfate, 25% ferric sulfate, 5% nano titanium dioxide, 50% mixed bacteria powder into gelatin hollow capsules, get microbial preparation.

[0047] Comparative example 2 (replace sodium thiosulfate, ferric sulfate, nano titanium dioxide with flocculating agent 3600DF purchased on the market, the rest of the method steps are consistent with example 1): S1: inoculate photosynthetic bacteria, yeast, nitrifying bacteria, bacillus subtilis into solid culture medium at 25℃ for activation, respectively get activated photosynthetic bacteria seed liquid, activated yeast seed liquid, activated nitrifying bacteria seed liquid, activated bacillus subtilis seed liquid; the pH of the solid culture medium is 7.4, the activation time is 24h; the solid culture medium includes the following components, by concentration: beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L, agar 15g / L, the rest is water;

[0048] S2: inoculate the activated photosynthetic bacteria seed liquid into solid culture medium, keep the light intensity at 3500Lx, culture for 48h, centrifugal, dry, get photosynthetic bacteria powder; inoculate the activated yeast seed liquid into solid culture medium, culture for 35h under 0.04MP pressure, centrifugal, dry, get yeast powder; inoculate the activated nitrifying bacteria seed liquid into solid culture medium, fermentation aeration rate 130%, culture for 36h under 0.045MP pressure, centrifugal, dry, get nitrifying bacteria powder; inoculate the activated bacillus subtilis seed liquid into solid culture medium, fermentation aeration rate 130%, culture for 36h under 0.04MP pressure, centrifugal, dry, get bacillus subtilis powder; the culture temperature is 28℃;

[0049] S3: mix 30% photosynthetic bacteria powder, 20% yeast powder, 30% nitrifying bacteria powder, 20% bacillus subtilis powder, get mixed bacteria powder;

[0050] S4: Take 2 parts of gelatin and add to 100 parts of water, stir and dissolve at 70°C, pour into 60 parts of mixed bacteria powder, keep at 60°C, stir at 1290 r / min for 0.5 h, ultrasonic dispersion for 30 min, wash, filter, and obtain gelatin microspheres coated with mixed bacteria powder;

[0051] S5: Fill 50% flocculating agent 3600DF and 50% gelatin microspheres into gelatin hollow capsules to obtain a microbial preparation.

[0052] Comparative Example 3 (changing the amount of sodium thiosulfate, ferric sulfate, nano titanium dioxide, and gelatin microspheres, and the remaining steps are consistent with Example 1): S1: Photosynthetic bacteria, yeast, nitrifying bacteria, and Bacillus subtilis were inoculated into solid culture medium at 25°C for activation, and activated photosynthetic bacteria seed liquid, activated yeast seed liquid, activated nitrifying bacteria seed liquid, and activated Bacillus subtilis seed liquid were obtained, respectively. The pH of the solid culture medium was 7.4, and the activation time was 24 h. The solid culture medium included the following components by concentration: beef extract 3 g / L, peptone 10 g / L, sodium chloride 5 g / L, agar 15 g / L, and the rest was water;

[0053] S2: The activated photosynthetic bacteria seed liquid was inoculated into the solid culture medium, and the light intensity was kept at 3500 Lx for 48 h of culture, centrifugation, and drying to obtain photosynthetic bacteria powder. The activated yeast seed liquid was inoculated into the solid culture medium and cultured under a pressure of 0.04 MP for 35 h, centrifuged, and dried to obtain yeast powder. The activated nitrifying bacteria seed liquid was inoculated into the solid culture medium, and the fermentation aeration rate was 130%, and the culture was carried out under a pressure of 0.045 MP for 36 h, centrifuged, and dried to obtain nitrifying bacteria powder. The activated Bacillus subtilis seed liquid was inoculated into the solid culture medium, and the fermentation aeration rate was 130%, and the culture was carried out under a pressure of 0.04 MP for 36 h, centrifuged, and dried to obtain Bacillus subtilis powder. The culture temperature was 28°C.

[0054] S3: Mix 30% photosynthetic bacteria powder, 20% yeast powder, 30% nitrifying bacteria powder, and 20% Bacillus subtilis powder to obtain mixed bacteria powder.

[0055] S4: Take 2 parts of gelatin and add to 100 parts of water, stir and dissolve at 70°C, pour into 60 parts of mixed bacteria powder, keep at 60°C, stir at 1290 r / min for 0.5 h, ultrasonic dispersion for 30 min, wash, filter, and obtain gelatin microspheres coated with mixed bacteria powder.

[0056] S5: Fill 35% sodium thiosulfate, 35% ferric sulfate, 10% nano titanium dioxide, and 20% gelatin microspheres into gelatin hollow capsules to obtain a microbial preparation.

[0057] Comparative Example 4 (changing the preparation process of gelatin microspheres, other steps are consistent with Example 1): S1: photosynthetic bacteria, yeast, nitrifying bacteria, Bacillus subtilis were inoculated in solid culture medium at 25℃ for activation, and activated photosynthetic bacteria seed liquid, activated yeast seed liquid, activated nitrifying bacteria seed liquid, and activated Bacillus subtilis seed liquid were obtained, respectively; the pH of the solid culture medium was 7.4, and the activation time was 24 h; the solid culture medium included the following components by concentration: beef extract 3 g / L, peptone 10 g / L, sodium chloride 5 g / L, agar 15 g / L, and the rest was water;

[0058] S2: the activated photosynthetic bacteria seed liquid was inoculated in the solid culture medium, the light intensity was kept at 3500 Lx, and the culture was carried out for 48 h, then centrifuged and dried to obtain photosynthetic bacteria powder; the activated yeast seed liquid was inoculated in the solid culture medium, and the culture was carried out for 35 h under a pressure of 0.04 MP, then centrifuged and dried to obtain yeast powder; the activated nitrifying bacteria seed liquid was inoculated in the solid culture medium, the fermentation aeration rate was 130%, and the culture was carried out for 36 h under a pressure of 0.045 MP, then centrifuged and dried to obtain nitrifying bacteria powder; the activated Bacillus subtilis seed liquid was inoculated in the solid culture medium, the fermentation aeration rate was 130%, and the culture was carried out for 36 h under a pressure of 0.04 MP, then centrifuged and dried to obtain Bacillus subtilis powder; the culture temperature was 28℃;

[0059] S3: 30% photosynthetic bacteria powder, 20% yeast powder, 30% nitrifying bacteria powder, and 20% Bacillus subtilis powder were mixed to obtain mixed bacteria powder;

[0060] S4: 2 parts of gelatin were added to 100 parts of water, stirred and dissolved at 70℃, then poured into 60 parts of mixed bacteria powder, kept at 60℃, stirred at 1290 r / min for 30 min, ultrasonic dispersed for 30 min, washed, filtered, kept at 60℃, stirred at 1290 r / min for 30 min, ultrasonic dispersed for 30 min, washed, filtered, and gelatin microspheres coated with mixed bacteria powder were obtained;

[0061] S5: 20% sodium thiosulfate, 25% ferric sulfate, 5% nano titanium dioxide, and 50% gelatin microspheres were filled into gelatin hollow capsules to obtain microbial preparations.

[0062] Experiment: the water from a certain carp farm was taken as the experimental water source, wherein the ammonia nitrogen was 1.35 mg / L; the microbial preparations prepared in Examples 1-3 and Comparative Examples 1-4 were added into the experimental water source, and the dosage was 1.5 g / L; the ammonia nitrogen content in the experimental water source after 2 days, 5 days, and 15 days of adding was measured, and the specific data are shown in the table below;

[0063] 2 days later / (mg / L) 5 days later / (mg / L) 15 days later / (mg / L) Example 1 0.48 0.53 0.27 Example 2 0.51 0.55 0.28 Example 3 0.49 0.54 0.27 Comparative Example 1 0.49 0.55 0.89 Comparative Example 2 0.74 0.91 0.62 Comparative Example 3 0.45 0.55 0.81 Comparative Example 4 0.51 0.59 0.58

[0064] Conclusion: from the above table, example 1 is the best scheme; comparative example 1 does not use gelatin to coat the mixed bacteria powder, which is equivalent to mixing the mixed bacteria powder with the reducing agent and then simultaneously putting it into the water body, the survival rate of microorganisms in the mixed bacteria powder is low, resulting in high ammonia nitrogen content in the water after 15 days, and the ammonia nitrogen removal effect is not long-lasting; comparative example 2 replaces sodium thiosulfate, ferric sulfate and nano titanium dioxide with a flocculant, and the effect is not as good as the microbial preparation prepared by the present application; comparative example 3 adjusts the addition amount of sodium thiosulfate, ferric sulfate, nano titanium dioxide and gelatin microspheres, because the addition amount of gelatin microspheres is reduced, there are fewer microorganisms in the water body, resulting in a relatively high ammonia nitrogen content after 15 days, which is not conducive to the survival of cultured organisms; in comparative example 4, the gelatin shell of the gelatin microspheres is thick and hydrolyzes slowly, resulting in a slow effect of ammonia nitrogen removal.

[0065] In summary, the scheme provided by the present application can prepare a microbial preparation for conditioning the water environment of aquatic culture, which has fast effect, long-lasting effect and is easy to use.

[0066] It should be noted that in this paper, relationship terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between the entities or operations. Moreover, the terms "include", "contain" or any other variant thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment.

[0067] Finally, it should be noted that: the above only describes the preferred embodiments of the present application, and is not used to limit the present application, although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A preparation process for a microbial preparation for conditioning the aquatic environment in aquaculture, characterized in that, Includes the following steps: S1: Add gelatin to water, stir to dissolve, add mixed bacterial powder, stir, ultrasonically disperse, wash, and filter to obtain gelatin microspheres coated with mixed bacterial powder; S2: Sodium thiosulfate, ferric sulfate, nano-titanium dioxide, and gelatin microspheres are filled into hollow capsules prepared from gelatin to obtain a microbial preparation; The microbial preparation comprises the following raw materials, by mass percentage: 18-25% sodium thiosulfate, 23-28% ferric sulfate, 3-6% nano titanium dioxide, and 45-55% gelatin microspheres. The mixed bacterial powder comprises the following raw materials, by mass percentage: 26-35% photosynthetic bacteria powder, 15-25% yeast powder, 25-35% nitrifying bacteria powder, and 18-25% Bacillus subtilis powder.

2. The preparation process of a microbial preparation for conditioning aquatic environments according to claim 1, characterized in that: The gelatin is water-soluble gelatin.

3. The preparation process of a microbial preparation for conditioning aquatic environments according to claim 1, characterized in that: The preparation of the photosynthetic bacteria powder includes the following steps: inoculating activated photosynthetic bacteria seed liquid into a solid culture medium, culturing it under a light intensity of 3000~4000Lx for 45~50h, centrifuging, drying, and obtaining photosynthetic bacteria powder.

4. The preparation process of a microbial preparation for conditioning aquatic environments according to claim 1, characterized in that: The preparation of the yeast powder includes the following steps: inoculating activated yeast seed liquid into a solid culture medium, culturing at a pressure of 0.02~0.05 MPa for 30~40 h, centrifuging, drying, and obtaining yeast powder.

5. The preparation process of a microbial preparation for conditioning aquatic environments according to claim 1, characterized in that: The preparation of the nitrifying bacteria powder includes the following steps: inoculating activated nitrifying bacteria seed liquid into a solid culture medium, fermenting with an aeration rate of 120-150%, culturing at a pressure of 0.02-0.05 MPa for 30-40 h, centrifuging, drying, and obtaining nitrifying bacteria powder.

6. The preparation process of a microbial preparation for conditioning aquatic environments according to claim 1, characterized in that: The preparation of the Bacillus subtilis powder includes the following steps: inoculating activated Bacillus subtilis seed liquid into a solid culture medium, fermenting with an aeration rate of 120-150%, culturing at a pressure of 0.02-0.05 MPa for 30-40 h, centrifuging, and drying to obtain Bacillus subtilis powder.

7. The preparation process of a microbial preparation for conditioning aquatic environments according to claim 3, characterized in that: The pH of the solid culture medium is 7-7.5; the solid culture medium comprises the following raw materials, in terms of concentration: beef extract 2.5-3.5 g / L, peptone 8-12 g / L, sodium chloride 3-6 g / L, agar 12-17 g / L, and the remainder is water.

8. The microbial preparation obtained by the preparation process of a microbial preparation for conditioning aquatic environments according to any one of claims 1 to 7.