Traditional Chinese medicine composition for treating diabetes and preparation method thereof

Abstract

The invention discloses a Chinese medicine composition for treating diabetes, belonging to the field of Chinese medicine, and comprising, by weight, 501-1746 parts of eucommia leaf extract; 885-2700 parts of mulberry leaf extract; 528-2600 parts of total glycosides of cistanche; 398-1880 parts of total flavonoids of kudzu root; and 620-2200 parts of extract of fructus ligustri lucidi. The eucommia leaf extract is an extract with phenolic acid and glycoside components greater than 50%; the mulberry leaf extract is an extract with flavonoid and alkaloid components greater than 50%; the total glycosides of cistanche refer to extracts extracted from cistanche medicinal materials and containing glycoside components with a weight percentage of more than 50%; and the total flavonoids of kudzu root refer to extracts extracted from kudzu medicinal materials and containing total flavonoid components with a weight percentage of more than 50%. The Ligustrum lucidum fruit extract is extracted from Ligustrum lucidum fruit, and the content of glycoside components and triterpene acid components is greater than 50% by weight.

Description

Technical Field

[0001] The present invention relates to the field of traditional Chinese medicine compositions. Background Art

[0002] Diabetes is a syndrome of metabolic disorders of sugar, protein, fat, water and electrolytes caused by various pathogenic factors such as genetic factors, immune dysfunction, microbial infection and its toxins, free radical toxins, mental factors, etc., which act on the body to cause pancreatic islet dysfunction and insulin resistance.

[0003] In recent years, some important Chinese medicines have been found to have good effects in treating diabetes, but the effect of a single Chinese medicine on the treatment of diabetes is still not ideal, and it is very important to prepare a Chinese medicine combination with a suitable formula.

[0004] Eucommia leaf extract is rich in phenolic acid components and lignan glycoside components, both of which have the activity of inhibiting lipase and glucosidase, and can improve human immunity and enhance physical fitness. The total alkaloids in mulberry leaf extract have a good effect of inhibiting glucose absorption, and the total flavonoids can improve vascular microcirculation and anti-oxidation. The total glycosides of Cistanche deserticola have a good effect of anti-oxidation, liver protection, and improvement of renal function. The total flavonoids of Pueraria root can protect blood vessels, improve microcirculation, and improve the body's immunity. In the extract of Ligustrum lucidum, triterpene acids can reduce insulin resistance, improve insulin sensitivity, and total glycosides improve blood circulation and improve immunity. The combination of five active extracts of traditional Chinese medicine can effectively control blood sugar concentration, reduce insulin resistance, protect liver and kidney function, improve human physical fitness, and have the effect of treating both the symptoms and the root causes. The extraction process of each of the above extracts has a crucial impact on its performance, and finding a suitable extraction process and a Chinese medicine composition has become a difficulty in preparing Chinese medicine for diabetes. Summary of the invention

[0005] The purpose of this application is to solve the problems existing in the prior art and disclose a Chinese medicine composition for treating diabetes, which is made of the following raw materials in parts by weight:

[0006] Eucommia ulmoides leaf extract: 501-1746

[0007] Mulberry leaf extract: 885-2700

[0008] Total glycosides of Cistanche deserticola: 528-2600

[0009] Total flavonoids of Pueraria root: 398-1880

[0010] Ligustrum lucidum extract: 620-2200

[0011] The eucommia leaf extract is prepared by extracting the eucommia leaves with water or aqueous ethanol, and the content of phenolic acid and glycoside components is greater than 50% by weight.

[0012] The mulberry leaf extract is prepared by refining mulberry leaves through water extraction. The active ingredients of the mulberry leaf extract are composed of total alkaloids and total flavonoids, and the content of the total active ingredients is greater than 50% by weight.

[0013] The total glycosides of Cistanche deserticola are extracted and refined from Cistanche deserticola with water or aqueous ethanol, and the content of total glycoside components is greater than 50% by weight.

[0014] The total flavonoids of Pueraria root are extracted and refined by water or aqueous ethanol, and the flavonoid content is greater than 50% by weight.

[0015] The extract of Ligustrum lucidum is extracted and refined by water or aqueous ethanol, and the content of glycoside components and triterpene acid components thereof is greater than 50% by weight.

[0016] In a specific embodiment, the Eucommia ulmoides leaf extract is prepared according to the following method:

[0017] 1) Take the Eucommia leaf powder, add 10-15 times the weight of 0%-70% ethanol solution, heat and reflux to extract 1-3 times, each time for 0.5-2 hours, and combine the extracts.

[0018] 2) The extract is concentrated under reduced pressure until it has no alcohol taste, and passed through a macroporous resin. First, it is eluted with deionized water until the effluent is transparent, and then eluted with a 20%-70% ethanol solution for 4-6 times the column volume, and the 20%-70% ethanol eluate is collected.

[0019] 3) Concentrate the 20%-70% ethanol eluate until it has no alcohol smell, pass it through an MCI GEL reverse phase resin column, elute with 0%-10% ethanol for 3-4 column volumes, discard, elute with 30%-60% ethanol for 5-6 column volumes, and perform HPLC detection. Collect the eluate rich in polyphenols and glycosides, concentrate and dry, and obtain a refined extract with a phenolic acid and total glycoside content of 50%-90%.

[0020] 4) A method for preparing an extract of Eucommia ulmoides leaves, characterized in that: the macroporous adsorption resin is AB-8 type or D101 type macroporous adsorption resin, and the MCI GEL reverse phase filler is 50-75u or 30-50u.

[0021] In a specific embodiment, the mulberry leaf extract is prepared according to the following method:

[0022] 1) Take mulberry leaf medicinal material, add 10-15 times deionized water according to weight, heat and reflux to extract 1-3 times, each time for 0.5-2 hours, and combine the extracts.

[0023] 2) Filter the extract through a macroporous resin, first elute with deionized water until the effluent is transparent, then elute with 20%-70% ethanol solution for 4-6 times the column volume, and collect the effluent and 20%-70% ethanol eluate.

[0024] 3) Pass the effluent through the cation exchange resin, first elute with deionized water until the effluent is transparent, then elute with pH 9-11 ammonia solution for 4-6 times the column volume, and collect the ammonia eluate.

[0025] 4) The ammonia eluate and the 20%-70% ethanol eluate are combined, concentrated and dried to obtain a mulberry leaf extract having a flavonoid and alkaloid content of 50%-80%.

[0026] In a specific embodiment, the total glycosides of Cistanche deserticola are prepared according to the following method:

[0027] 1) Take the Cistanche deserticola powder, add 10-15 times the weight of 30%-90% ethanol solution, heat and reflux to extract 1-3 times, each time for 0.5-2 hours, and combine the extracts.

[0028] 2) Reduce the pressure and concentrate the extract until it has no alcohol taste. Pass it through a macroporous resin and elute it with water or an ethanol solution with a concentration not exceeding 10% until the effluent is transparent. Then elute it with a 20%-60% ethanol solution for 4-6 times the column volume and collect the 20%-60% ethanol eluate.

[0029] 3) The 20%-60% ethanol eluate was concentrated until there was no alcohol smell, passed through an MCI GEL reverse phase resin column, eluted with 5%-20% ethanol for 3-4 column volumes, discarded, and eluted with 40%-70% ethanol for 5-6 column volumes, and tested by HPLC. The eluate rich in echinacoside, cistancheside, verbascoside and isovalascoside was collected, concentrated and dried to obtain 50%-90% total glycosides of Cistanche.

[0030] 4) A method for preparing Cistanche deserticola, characterized in that: the macroporous adsorption resin is AB-8 type or D101 type macroporous adsorption resin, and the MCI GEL reverse phase filler is 50-75u or 30-50u.

[0031] In a specific embodiment, the pueraria total flavonoids are prepared according to the following method:

[0032] 1) Take Pueraria root, add 10-15 times the weight of 30%-90% ethanol solution, heat and reflux to extract 1-3 times, each time for 0.5-2 hours, and combine the extracts.

[0033] 2) The extract is concentrated under reduced pressure until it has no alcohol taste, and then passed through a macroporous resin. It is first eluted with water or an ethanol solution with a concentration not exceeding 10% until the effluent is transparent, and then eluted with an ethanol solution with a concentration of 30%-70% for 4-6 times the column volume. HPLC detection is performed, and the eluate rich in flavonoids such as puerarin, 3-hydroxypuerarin, 3-methoxypuerarin, and daidzein is collected, concentrated and dried to obtain 50%-90% total flavonoids from Pueraria lobata.

[0034] 4) A method for preparing total flavonoids from kudzu root, characterized in that: the macroporous adsorption resin is AB-8 type or D101 type macroporous adsorption resin.

[0035] In a specific embodiment, the Ligustrum lucidum extract is prepared according to the following method:

[0036] 1) Take Ligustrum lucidum fruit, add 10-15 times of 60%-90% ethanol solution by weight, heat and reflux to extract 1-3 times, each time for 0.5-2 hours, and combine the extracts;

[0037] 2) Pass the extract through the macroporous resin, elute with an ethanol solution of the same concentration until the effluent is colorless, then concentrate to an ethanol concentration of less than 40%-50%, leave overnight, filter, and collect a light yellow filter cake;

[0038] 3) The filtrate is further concentrated until there is no alcohol smell, and then passed through a macroporous resin. It is first eluted with water or an ethanol solution with a concentration not exceeding 10% until the effluent is transparent, and then eluted with an ethanol solution with a concentration of 30%-70% for 4-6 times the column volume. After HPLC detection, the eluate rich in glycoside components such as salidroside and salidroside is collected, concentrated and dried, and evenly mixed with the above-mentioned light yellow filter cake to obtain a refined extract of glycosides and triterpene acids with a content of 50%-90%.

[0039] 4) A method for preparing Ligustrum lucidum fruit, characterized in that: the macroporous adsorption resin is AB-8 type or D101 type macroporous adsorption resin.

[0040] The present application provides a composition of effective ingredients of five medicinal materials, which has strong protein tyrosine phosphatase PTP1B inhibitory activity and can be used to prepare drugs for preventing and treating diseases caused by insulin resistance such as diabetes.

[0041] The total inhibitory activity IC of the composition with the best ratio was 1.1777 W / cm3 after in vitro PTP1B enzyme inhibition test. 501.8ug / mL. In addition, animal experiments have found that the present invention has a significant effect of lowering blood sugar and reducing water intake and urine output in diabetic mouse models induced by alloxan, and that after three months of administration, the blood sugar levels of more than 90% of mice can be kept basically stable within one month by maintaining a low-dose administration. It can be seen that the Chinese medicine composition provided by the present invention has a strong PTP1B inhibitory activity, and is feasible for the development and preparation of drugs and health products for the prevention and treatment of diseases caused by insulin resistance such as diabetes.

[0042] The results of the pharmacodynamic and toxicological studies show that the composition of the present invention has a significant hypoglycemic effect, and its pharmacodynamic activity is significantly better than that of the single herbal extracts in the composition, and it is safe and has no obvious toxic and side effects.

[0043] The composition can effectively control blood sugar levels, improve liver and kidney functions, increase insulin sensitivity, reduce insulin resistance, and enhance body immunity. According to the mechanism of action and activity intensity of the composition, it can be used as a preventive and therapeutic drug for type II diabetes.

[0044] The invention has a simple preparation process and is easy to mass produce. The effective component content of the obtained refined extract is as high as 50% or more. The technology is advanced and the quality is controllable. The invention adopts the latest materials combined with optimized process conditions to greatly improve the quality of the product, and the operation is simple and easy to scale up. DETAILED DESCRIPTION

[0045] The present invention is described in detail below.

[0046] 1. Extraction

[0047] 1. Extraction of Eucommia ulmoides leaf extract

[0048] 1) Take the Eucommia leaf powder, add 10 times the weight of 50% ethanol solution, heat and reflux to extract 3 times, each time for 1 hour, and combine the extracts.

[0049] 2) The extract was concentrated under reduced pressure until it had no alcohol smell, and passed through D101 macroporous resin. It was first eluted with deionized water until the effluent was transparent, and then eluted with 60% ethanol solution for 6 times the column volume, and the 60% ethanol eluate was collected.

[0050] 3) The 60% ethanol eluate was concentrated until there was no alcohol smell, passed through a 50-70 micron MCI GEL reverse phase resin column, eluted with 10% ethanol for 4 times the column volume, discarded, and eluted with 50% ethanol for 5-6 times the column volume. After HPLC detection, the eluate rich in polyphenols and glycosides was collected, concentrated and dried to obtain a refined extract with a phenolic acid and total glycoside content of 68%.

[0051] 2. Extraction of mulberry leaf extract

[0052] 1) Take mulberry leaf medicinal material, add 12 times deionized water according to its weight, heat and reflux to extract twice, each time for 1.5 hours, and combine the extracts.

[0053] 2) Filter the extract and pass it through AB-8 macroporous resin. First, elute with deionized water until the effluent is transparent. Then, elute with 55% ethanol solution for 6 times the column volume. Collect the effluent and 55% ethanol eluate.

[0054] 3) Pass the effluent through the cation exchange resin, first elute with deionized water until the effluent is transparent, then elute with PH10 ammonia solution for 5 times the column volume, and collect the ammonia eluate.

[0055] 4) The ammonia eluate and the 55% ethanol eluate were combined, concentrated and dried to obtain a mulberry leaf extract with a flavonoid and alkaloid content of 65%.

[0056] 2. Extraction of total glycosides from Cistanche deserticola

[0057] The total glycosides of Cistanche deserticola are prepared according to the following method:

[0058] 1) Take the powder of Cistanche deserticola, add 10 times the weight of 50% ethanol solution, heat and reflux to extract 3 times, each time for 1 hour, and combine the extracts.

[0059] 2) The extract was concentrated under reduced pressure until it had no alcohol smell, and passed through D101 macroporous resin. First, it was eluted with water until the effluent was transparent, and then eluted with 50% ethanol solution for 5 times the column volume, and the 50% ethanol eluate was collected.

[0060] 3) The 50% ethanol eluate was concentrated until there was no alcohol smell, passed through an MCI GEL reverse phase resin column, eluted with 10% ethanol for 3-4 column volumes, discarded, and eluted with 40% ethanol for 6 column volumes. After HPLC detection, the eluate rich in echinacoside, cistancheside, verbascoside and isovalascoside was collected, concentrated and dried to obtain a total glycoside of Cistanche deserticola with a content of 71%.

[0061] 4. Pueraria root total flavonoids extraction

[0062] The pueraria total flavonoids are prepared according to the following method:

[0063] 1) Take Pueraria root, add 10 times the weight of 50% ethanol solution, heat and reflux to extract twice, each time for 1.5 hours, and combine the extracts.

[0064] 2) The extract was concentrated under reduced pressure until it was free of alcohol, and passed through AB-8 macroporous resin. First, it was eluted with water until the effluent was transparent, and then eluted with 50% ethanol solution for 6 times the column volume.

[0065] 3) The 50% ethanol eluate was concentrated until there was no alcohol smell, passed through an MCI GEL reverse phase resin column, eluted with 10% ethanol for 3-4 column volumes, discarded, and eluted with 40% ethanol for 6 column volumes. After HPLC detection, the eluate rich in flavonoids such as puerarin, 3-hydroxypuerarin, 3-methoxypuerarin, and daidzein was collected, concentrated and dried to obtain 68% total flavonoids of Pueraria lobata.

[0066] 5. Extraction of Ligustrum lucidum extract

[0067] The Ligustrum lucidum extract is prepared according to the following method:

[0068] 1) Take Ligustrum lucidum fruit, add 12 times the weight of 90% ethanol solution, heat and reflux to extract twice, each time for 1.5 hours, and combine the extracts.

[0069] 2) The extract was passed through D101 macroporous resin and eluted with ethanol solution of the same concentration until the effluent was colorless, then concentrated to an ethanol concentration of less than 50%, left overnight, filtered, and the light yellow filter cake was collected.

[0070] 3) The filtrate is further concentrated until there is no alcohol smell, and then passed through a macroporous resin, first eluted with a 10% ethanol solution until the effluent is transparent, and then eluted with a 50% ethanol solution for 6 times the column volume. After HPLC detection, the eluate rich in glycoside components such as terunculoside and salidroside is collected, concentrated and dried, and evenly mixed with the above-mentioned light yellow filter cake to obtain a glycoside and triterpene acid extract with a total content of 62%.

[0071] The various extracts prepared and the extracts prepared were subjected to the following tests.

[0072] 2. PTP1B Inhibitory Activity Test

[0073] (I) Test principle: The catalytic domain of human protein tyrosine phosphatase 1B (hPTPlB) is expressed in the E. coli system using molecular biological methods. The purified hPTPlB recombinant protein can hydrolyze the phosphoester bond of the substrate p-nitrophenyl phosphate (PNPP), and the obtained product has strong light absorption at 410nm. Therefore, the change of light absorption at 410nm can be directly detected to observe the change of enzyme activity and the inhibition of enzyme activity by the compound. The standard activity test system is as follows: 10mM tris hydrochloride, pH7.6, 10mM p-nitrophenyl phosphate, 2% dimethyl sulfoxide (DMSO), 100nM hPTPlB.

[0074] (ii) Observation indicators: Dynamically measure the light absorption at a wavelength of 410 nlm for 3 minutes. The slope of the first-order reaction of the kinetic curve is used as an indicator of enzyme activity.

[0075] (III) Sample test: All tested samples of Eucommia leaf extract, mulberry leaf extract, Cistanche deserticola total glycosides, Pueraria root total flavonoids and Ligustrum lucidum extract and specific ratio composition were prepared into 1mg / ml solution with DMSO, 2uL of sample was taken and placed in a microwell, and then 2uL of substrate PNPP, 93uL of buffer, 5uL of PTP1B enzyme were added, and the sample was placed in a microplate reader after shaking to read the data, and the inhibition rate was obtained after data processing. When the inhibition activity was higher than 50%, a series of concentrations such as 10ug / mL, 5ug / mL, 1ug / mL, 0.5ug / mL, 0.1ug / mL, etc. were prepared to test the inhibition rate, and the IC50 value was obtained after data processing. The positive control was sodium orthovanadate (4ug / mL DMSO solution).

[0076] Test results: The inhibitory activity IC50 of Eucommia ulmoides leaf extract was 6.1ug / mL, the inhibitory activity IC50 of mulberry leaf extract was 2.9ug / mL, the inhibitory activity IC50 of Cistanche deserticola total glycosides was 4.6ug / mL, the inhibitory activity IC50 of Pueraria lobata total flavonoids was 3.8ug / mL, and the inhibitory activity IC50 of Ligustrum lucidum fruit extract was 2.6ug / mL.

[0077] The total inhibitory activity IC50 of the mixed composition of the five ingredients is 1.8ug / mL, wherein the ratio of Eucommia ulmoides leaf extract, mulberry leaf extract, Cistanche deserticola total glycosides, Pueraria lobata total flavonoids, and Ligustrum lucidum fruit extract in the composition is 0.5:1:0.5:1:1.

[0078] 3. Pharmacological Activity Experiment

[0079] 1. Effects of the extract and combination on glucose tolerance in alloxan-induced diabetic model mice:

[0080] 80 male alloxan-induced diabetic model mice, weighing 23-26 g, with normal saline as blank control; 60 mg / kg sodium orthovanadate aqueous solution as positive control; test samples were Eucommia leaf extract, mulberry leaf extract, Cistanche total glycosides, Pueraria total flavonoids, Ligustrum lucidum extract and a combination of the five in equal mass ratio, prepared according to the method of this embodiment, the model mice were randomly divided into 8 groups, 10 mice in each group, and physiological saline, sodium orthovanadate aqueous solution (60 mg / kg) and 1% sodium carboxymethyl cellulose suspension of the test sample (200 mg / kg) were respectively administered once a day for 4 consecutive weeks. After the last administration, the mice were fasted for 12 hours, glucose solution (2 g / kg) was administered, and blood was collected 30 minutes later to measure blood sugar.

[0081] The test results are shown in the table below.

[0082]

[0083] As can be seen from the table above, for the diabetic mouse model induced by alloxan, the five Chinese herbal extracts all have a certain hypoglycemic effect, and the mulberry leaf extract combined with the Ligustrum lucidum extract group is more significant, reducing blood sugar by 32% and 31% respectively, and the combination of the five has a more obvious synergistic effect, reducing blood sugar by 43%. Compared with the sodium orthovanadate control group, some of the single group groups have lower efficacy, some have equivalent efficacy, and the efficacy of the combination group is much better than the sodium orthovanadate control group.

[0084] 2 Effects of five kinds of Chinese herbal extracts on glucose tolerance in alloxan-induced diabetic model mice:

[0085] 130 male alloxan-induced diabetic model mice, weighing 23-26g, were used as blank control with normal saline; 60mg / kg sodium orthovanadate aqueous solution was used as positive control; five kinds of extracts were set at two doses, high and low, respectively, and two of the extracts were changed, while the other three extracts remained unchanged. First, based on 1:1:1:1:1 as the benchmark, the test sample combinations were 0.5:0.5:1:1:1, 0.5:1:0.5:1:1, 0.5:1:1:0.5:1:1, 0.5:1:1:0.5:1, 0.5:1:1:0.5:1, 0.5:1:1:1:0.5, 1:0.5:0.5:1:1, 1:0.5:1:0.5:1, 1:0.5:1:1:0.5, 1:1:0.5:0.5:1, 1:1:0.5:1:0.5, 1:1:1:0.5:0.5, a total of 10 groups, screened and optimized the best ratio. According to the method of Example 1, the model mice were randomly divided into 13 groups, 10 in each group, and administered with physiological saline, sodium orthovanadate aqueous solution (60 mg / kg) and 1% sodium carboxymethyl cellulose suspension (200 mg / kg) of the test sample, once a day for 4 consecutive weeks. After the last administration, they fasted for 12 hours, administered with glucose solution (2 g / kg), and blood was taken 30 minutes later to measure blood sugar.

[0086] The test results are shown in the following table:

[0087]

[0088]

[0089] As can be seen from the above table, for the alloxan-induced diabetic mouse model, the compositions of different proportions all have a more obvious hypoglycemic effect. The different proportion groups are better than the sodium orthovanadate control group, and the efficacy of the 0.5:1:0.5:1:1 composition group is much better than the sodium orthovanadate control group, which is the best proportion composition, reducing blood sugar by 46%.

Claims

1. A Chinese medicine composition for treating diabetes, characterized in that: According to weight parts, it is composed of the following components: Eucommia ulmoides leaf extract: 501-1746; Mulberry leaf extract: 885-2700; Total glycosides of Cistanche deserticola: 528-2600; Total flavonoids of Pueraria root: 398-1880; Ligustrum lucidum extract: 620-2200; The extraction method of the Eucommia leaf extract is as follows: 1) taking Eucommia leaf medicinal material powder, adding 10 times of 50% ethanol solution by weight, heating and refluxing extraction 3 times, each time for 1 hour, and combining the extracts; 2) reducing pressure and concentrating the extract until there is no alcohol taste, passing through D101 macroporous resin, first eluting with deionized water until the effluent is transparent, then eluting with 60% ethanol solution for 6 times column volume, and collecting 60% ethanol eluate; 3) concentrating the 60% ethanol eluate until there is no alcohol taste, passing through a 50-70 micron MCI GEL reverse phase resin column, eluting with 10% ethanol for 4 times column volume, discarding, eluting with 50% ethanol for 5-6 times column volume, HPLC detection, collecting the eluate rich in polyphenols and glycosides, concentrating and drying, and obtaining the Eucommia leaf extract; The extraction method of the mulberry leaf extract is as follows: 1) taking mulberry leaf medicinal material, adding 12 times of deionized water according to weight, heating and refluxing extraction twice, each time for 1.5 hours, and combining the extracts; 2) filtering the extract, passing through AB-8 macroporous resin, first eluting with deionized water until the effluent is transparent, then eluting with 55% ethanol solution for 6 times of column volume, collecting the effluent and 55% ethanol eluate; 3) passing the effluent through a cation exchange resin, first eluting with deionized water until the effluent is transparent, then eluting with PH10 ammonia solution for 5 times of column volume, and collecting the ammonia eluate; 4) combining the ammonia eluate and the 55% ethanol eluate, concentrating and drying to obtain the mulberry leaf extract; The extraction method of total glycosides of Cistanche deserticola comprises the following steps: 1) taking Cistanche deserticola medicinal material powder, adding 10 times of 50% ethanol solution according to weight, heating and refluxing extraction for 3 times, each time for 1 hour, and combining the extracts; 2) reducing pressure and concentrating the extract until there is no alcohol taste, passing through D101 macroporous resin, first eluting with water until the effluent is transparent, then eluting with 50% ethanol solution for 5 times of column volume, and collecting 50% ethanol eluate; 3) concentrating the 50% ethanol eluate until there is no alcohol taste, passing through MCI GEL reverse phase resin column, eluting with 10% ethanol for 3-4 times of column volume, discarding, eluting with 40% ethanol for 6 times of column volume, and performing HPLC detection, collecting the eluate rich in echinacoside, cistanche deserticola, verbascoside and isovalascoside, concentrating and drying, and obtaining total glycosides of Cistanche deserticola; The extraction method of total flavonoids of kudzu root is as follows: 1) taking kudzu root medicinal material, adding 10 times of 50% ethanol solution according to weight, heating and refluxing extraction twice, each time for 1.5 hours, and combining the extracts; 2) reducing pressure and concentrating the extract until there is no alcohol taste, passing through AB-8 macroporous resin, first eluting with water until the effluent is transparent, and then eluting with 6 times column volume of 50% ethanol solution; 3) concentrating the 50% ethanol eluate until there is no alcohol taste, passing through MCI GEL reverse phase resin column, eluting with 10% ethanol for 3-4 times column volume, discarding, eluting with 40% ethanol for 6 times column volume, HPLC detection, collecting the eluate rich in puerarin, 3-hydroxypuerarin, 3-methoxypuerarin and daidzein, concentrating and drying, and obtaining total flavonoids of kudzu root; The extraction method of the Ligustrum lucidum extract is as follows: 1) taking Ligustrum lucidum medicinal material, adding 12 times of 90% ethanol solution by weight, heating and refluxing extraction twice, each time for 1.5 hours, and combining the extracts; 2) passing the extract through D101 macroporous resin, eluting with ethanol solution of the same concentration until the effluent is colorless, then concentrating to an ethanol concentration of less than 50%, leaving overnight, filtering, and collecting a light yellow filter cake; 3) continuing to concentrate the filtrate until there is no alcohol taste, passing through a macroporous resin, first eluting with 10% ethanol solution until the effluent is transparent, then eluting with 50% ethanol solution for 6 times of column volume, HPLC detection, collecting the eluate rich in terunguside and salidroside, concentrating and drying, and uniformly mixing with the above-mentioned light yellow filter cake to obtain the Ligustrum lucidum extract.

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