Method for regulating the incubation period of t. obscurus embryos based on effective accumulated temperature model

Abstract

The present application relates to the technical field of aquaculture, and in particular to a method and system for regulating the incubation period of dark-striped pufferfish embryos based on an effective accumulated temperature model, an electronic device, and a storage medium. The method comprises the following steps: obtaining a target incubation period based on the incubation environment temperature; obtaining the actual expected incubation temperature for each day based on the effective accumulated temperature model and the target incubation period; obtaining the current actual incubation water temperature; and adjusting the current actual incubation water temperature to approach the actual expected incubation temperature for the day using a fuzzy control system. The present application uses a fuzzy PID temperature regulation algorithm for fish embryo incubation based on an effective accumulated temperature model, thereby achieving intelligent regulation of the water temperature for factory-scale embryo incubation.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of aquaculture, and particularly relates to a dark-striped pufferfish embryo hatching cycle regulation method and system based on an effective accumulated temperature model, an electronic device and a storage medium. BACKGROUND

[0002] A suitable hatching temperature is crucial for the study of fish embryo development, and sufficient and high-quality embryo hatching is the beginning of the fish farming industry and the guarantee of the subsequent industrial chain. Dark-striped pufferfish (Takifugu obscurus) is a kind of high-nutrition and high-value aquatic product, belonging to the family Tetraodontidae of the order Tetraodontiformes, and mainly distributed in the coastal waters of China and the middle and lower reaches of the Yangtze River. It is a kind of brackish water migratory fish. The meat of pufferfish is tender, and the pufferfish toxin extracted from the body has the effects of analgesia, sedation and antispasticity, and has high medical value. It is a freshwater farming species with broad prospects for comprehensive utilization, and the hatching of its embryo is of great significance. In 2020, the total cultivation amount of the Chinese pufferfish farming industry reached 26757 tons, with high economic benefits and broad market demand.

[0003] Temperature is an important factor affecting the growth and development of fish, especially in the embryonic development stage. The feeding organs in the body have not yet developed and cannot complete normal feeding activities at this time, so the nutrients required for growth and development are provided by the yolk. Therefore, the embryo development is weakly dependent on feeding and strongly dependent on the environment. The water temperature of the breeding environment can affect the activity of biological enzymes in the embryo, and then change the physiological metabolism level by affecting the degree of enzyme reaction to adjust its survival condition and development speed. That is, under certain environmental conditions other than temperature, the water temperature of the hatching environment plays a decisive role in the hatching rate and hatching time of the embryo. In order to ensure that the fry production is on time and sufficient, a controllable and suitable hatching environment is provided for the embryo to adjust its development cycle, and it has important research significance to the embryo hatching rate and deformity rate through certain technical means.

[0004] Traditional breeders tend to use manual methods for breeding production operations, such as relying on traditional data collection methods and manually adjusting the water temperature according to personal experience. Such production mode cannot dynamically adjust the production environment according to the production demand. In order to solve the technical problem, a dark-striped pufferfish embryo hatching cycle regulation method based on an effective accumulated temperature model is proposed. SUMMARY

[0005] In order to solve the technical problems existing in the prior art, the present application provides a dark-striped pufferfish embryo hatching cycle regulation method, system, electronic device and storage medium based on an effective accumulated temperature model.

[0006] To achieve the above object, the embodiment of the present application provides the following technical scheme:

[0007] In a first aspect, in an embodiment provided by the present application, a method for regulating the incubation period of the T. obscurus embryo based on an effective accumulated temperature model is provided, which comprises the following steps:

[0008] Based on the incubation environment temperature, a target incubation period is obtained;

[0009] According to the effective accumulated temperature model and the target incubation period, an actual expected incubation temperature of each day is obtained;

[0010] The current actual incubation water temperature is obtained, and a fuzzy control system is used to adjust the current actual incubation water temperature to approach the actual expected incubation temperature of the day.

[0011] As a further scheme of the present application, the target incubation period is obtained by calculation through the following formula:

[0012] N=78.905 / (T-7.6033)

[0013] In the formula, N is the target incubation period, and T is the incubation environment temperature.

[0014] As a further scheme of the present application, the actual expected incubation temperature of the nth day is obtained by calculation through the following formula:

[0015]

[0016] In the formula, T d e is the actual expected incubation temperature of the nth day; is the actual average temperature of the ith day; is the biological zero; is the total effective accumulated temperature.

[0017] As a further scheme of the present application, the total effective accumulated temperature is obtained by calculation through the following formula:

[0018]

[0019] In the formula, T ieff is the effective accumulated temperature of the ith day in the current growth stage.

[0020] As a further scheme of the present application, the current actual incubation water temperature is obtained, and a fuzzy control system is used to adjust the current actual incubation water temperature to approach the actual expected incubation temperature, comprising:

[0021] The current actual incubation water temperature is obtained and the actual expected incubation temperature T d eError value e and error change rate ec between them;

[0022] The current actual incubation water temperature , error value e and change rate ec are quantified and input into a fuzzy controller to obtain a fuzzy quantity 、 and three outputs;

[0023] According to the fuzzy quantity 、 and , the 、 and of the PID controller are adjusted respectively;

[0024] The adjusted PID controller controls and adjusts the water temperature of the incubation pond.

[0025] In a second aspect, in another embodiment provided by the present application, a dark mottled pufferfish embryo incubation period regulation system based on an effective accumulated temperature model is provided, which comprises a target incubation period acquisition module, an actual expected incubation temperature acquisition module and a temperature adjustment module.

[0026] The target incubation period acquisition module is configured to acquire a target incubation period based on an incubation environment temperature.

[0027] The actual expected incubation temperature acquisition module is configured to acquire an actual expected incubation temperature of each day according to an effective accumulated temperature model and the target incubation period.

[0028] The temperature adjustment module is configured to acquire a current actual incubation water temperature and adjust the current actual incubation water temperature to be close to the actual expected incubation temperature by using a fuzzy control system.

[0029] In a third aspect, in another embodiment provided by the present application, an electronic device is provided, which comprises a memory and a processor, the memory stores a computer program, and the processor loads and executes the computer program to realize the steps of the dark mottled pufferfish embryo incubation period regulation method based on the effective accumulated temperature model.

[0030] In a fourth aspect, in another embodiment provided by the present application, a storage medium is provided, which stores a computer program, and the computer program is loaded and executed by a processor to realize the steps of the dark mottled pufferfish embryo incubation period regulation method based on the effective accumulated temperature model.

[0031] The technical solution provided by the present application has the following beneficial effects:

[0032] The application provides a dark striped pufferfish embryo hatching cycle regulation method and system based on an effective accumulated temperature model, an electronic device and a storage medium.

[0033] These aspects or other aspects of the present application will become apparent from the detailed description given below. It should be understood that the detailed description and specific examples, while indicating certain embodiments of the application, are given by way of illustration only, and are not by way of limitation. BRIEF DESCRIPTION OF DRAWINGS

[0034] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the drawings needed in the embodiment or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and those skilled in the art can also obtain other embodiments according to these drawings without creative labor.

[0035] Figure 1 The flow chart of the dark striped pufferfish embryo hatching cycle regulation method based on the effective accumulated temperature model of an embodiment of the present application.

[0036] Figure 2 The specific flow chart of the dark striped pufferfish embryo hatching cycle regulation method based on the effective accumulated temperature model of an embodiment of the present application.

[0037] Figure 3 The control flow chart of the step S30 of the dark striped pufferfish embryo hatching cycle regulation method based on the effective accumulated temperature model of an embodiment of the present application.

[0038] Figure 4 The structure block diagram of the dark striped pufferfish embryo hatching cycle regulation system based on the effective accumulated temperature model of an embodiment of the present application.

[0039] In the figure: target hatching cycle acquisition module-100, actual expected hatching temperature acquisition module-200, temperature adjustment module-300. DETAILED DESCRIPTION

[0040] With reference to the accompanying drawings, the technical solutions in the embodiments of the present application will be clearly and completely described below, obviously, the described embodiments are some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work belong to the protection scope of the present application.

[0041] The flowcharts shown in the drawings are only illustrative, not necessarily including all the contents and operations / steps, and not necessarily executed in the described order. For example, some operations / steps can also be decomposed, combined or partially merged, so that the actual execution order can be changed according to the actual situation.

[0042] It should be understood that the terms used in the present application description herein are only for the purpose of describing specific embodiments and are not intended to limit the present application. As used in the present application description and the appended claims, unless otherwise clear from the context, the singular forms "a", "an" and "the" are intended to include the plural forms.

[0043] Specifically, the embodiments of the present application are further described below with reference to the accompanying drawings.

[0044] Please refer to Figure 1 , Figure 1 is a flowchart of a method for regulating the incubation period of the dark marbled pufferfish embryo based on an effective accumulated temperature model, as shown in Figure 1 The method for regulating the incubation period of the dark marbled pufferfish embryo based on an effective accumulated temperature model includes steps S10 to S30.

[0045] Effective accumulated temperature is the total effective heat required by a living being at a certain growth stage. In aquaculture, effective accumulated temperature refers to the part of the daily temperature that exceeds the minimum temperature requirement (Zero Celsius) for normal growth of fish, which can promote the growth and development of fish. Effective accumulated temperature in aquaculture is very important for fish growth and reproduction, and it reflects the linear relationship between the development of the living being at the current growth stage and temperature. It is one of the important indicators reflecting the development of the living being, and understanding effective accumulated temperature can help aquaculture practitioners choose suitable fish species, master the best breeding time and method, and thus improve the breeding efficiency of fish.

[0046] wherein the effective accumulated temperature of each day can be obtained by the following formula:

[0047]

[0048] In the formula, is the effective accumulated temperature of the i-th day in the current growth stage, Let be the average temperature on day i. This is the lowest temperature threshold at which an organism begins to develop, also known as biological zero. Below this temperature, the embryo will stop developing.

[0049] The cumulative effective accumulated temperature for this growth stage can be obtained by summing the daily effective accumulated temperature. The formula is as follows:

[0050]

[0051] In the formula T ieff T represents the effective accumulated temperature on day i within the current growth stage; N represents the number of days required for the organism to complete the current growth stage; T represents the effective accumulated temperature on day i within the current growth stage. iavg T represents the average temperature on day i. zero This is biological zero degrees Celsius; below this temperature, embryos cease development. count This represents the total effective accumulated temperature for this growth stage, signifying the total effective accumulated temperature required to complete this stage of development.

[0052] Assuming the average daily temperature remains constant, further derivation yields T. ieff When it is a constant value, through T count and T ieff Mathematical calculations are performed to determine the required period N for completing this growth stage at that temperature. Reversing this logic, after determining a reasonable embryo incubation period, the above theory is used to calculate the desired incubation temperature to meet the target period. The incubation temperature is then adjusted to control the embryo incubation period.

[0053] Total effective accumulated temperature T count This reflects the total effective temperature required for the organism to complete a certain growth stage. This value can be calculated by accumulating the effective accumulated temperature each day. Based on experiments, the T value for the embryonic hatching stage of the dark-spotted pufferfish was obtained. count The biological zero temperature (T) of the dark-spotted pufferfish is 78.905 degrees Celsius. zero The value is 7.6033℃. Subsequently, the expected effective accumulated temperature value for each day can be calculated using the target incubation period and the total effective accumulated temperature, and the expected incubation environment water temperature can be calculated using the calculated expected effective accumulated temperature value.

[0054] S10. Based on the incubation environment temperature, obtain the target incubation period.

[0055] Specifically, as shown in Table 1, there were no significant differences in hatching rate and survival rate of embryos within the temperature range of 19–23℃, but there were significant differences in hatching cycle. The results indicate that embryonic development speed is positively correlated with hatching water temperature, while hatching cycle is negatively correlated with hatching temperature. Therefore, within a suitable temperature range, the hatching cycle can be controlled by adjusting the temperature of the hatching environment.

[0056] Table 1 Hatching of embryos at different incubation temperatures

[0057] Table 1 Hatching of embryos at different incubation temperatures

[0058]

[0059] The effective accumulated temperature model reflects the relationship between the hatching period N and the hatching environment temperature T. Because the parameters derived from the effective accumulated temperature model (the total amount of effective accumulated temperature and biological zero) have important biological significance, the model is easy to calculate, generally has good fitting with experimental data, and can be analogized to other fish embryo hatching work, and has stronger generalization. The specific effective accumulated temperature model is: N = 78.905 / (T-7.6033). The model reflects the relationship between the hatching period N and the hatching environment temperature T. Through the actual production situation of the production site and the search for related papers on the hatching of T. narmata embryos, the model is verified.

[0060] S20, according to the effective accumulated temperature model and the target hatching period, to obtain the actual expected hatching temperature every day;

[0061] In the embodiments of the present application, specifically, after a reasonable target hatching period N is determined, the accumulated temperature amount T required every day in the ideal case for the growth stage can be calculated according to the effective accumulated temperature model ieff , and the accumulated temperature amount T ieff is obtained by subtracting the average temperature and the biological zero, so the theoretical expected hatching temperature T ieff that meets the hatching period can be obtained by adding the accumulated temperature amount T e and the biological zero. The theoretical expected hatching temperature T e is used as the target value for temperature regulation and control operation of the water temperature in the buffer tank. However, due to factors such as winter climate and pipe water supply, the water temperature is reduced, resulting in a difference between the actual temperature and the guide temperature, which cannot be completely consistent with the theoretical temperature, so it is necessary to dynamically adjust the subsequent actual expected hatching temperature T d e according to the existing accumulated temperature condition to ensure that the embryos are hatched within the target time. The actual expected hatching temperature T d e of the nth day is obtained according to the accumulated temperature condition of the previous n-1 days.

[0062] It should be noted that T eis a theoretical value calculated according to the total amount of effective accumulated temperature and the target period N days. However, due to factors such as water delivery pipeline and air temperature, the actual temperature cannot reach the calculated T e . However, the temperature conditions of the previous n-1 days are established facts and cannot be changed, so according to the n-1 day conditions, the actual expected incubation temperature T d that can meet the production target of completing incubation within N days in the remaining days is recalculated. e .

[0063] The actual expected incubation temperature of the n-th day is obtained by the following formula:

[0064]

[0065] In the formula, T d e is the actual expected incubation temperature of the n-th day calculated according to the existing accumulated temperature; is the actual average temperature of the i-th day. The actual accumulated temperature of each day is obtained by subtracting the biological zero from the actual temperature of each day. The actual accumulated temperature of the previous n days is added to the total amount of effective accumulated temperature to calculate the accumulated temperature amount required in the next N-n+1 days, and then the biological zero is added to calculate the actual expected incubation temperature T d e .

[0066] In the formula, T d e should meet the following range requirements:

[0067]

[0068] In the formula, is the upper limit of the water temperature in this incubation period; is the lower limit of the water temperature in this incubation period. The two values of Tmax and Tmin are obtained by searching the literature related to the incubation of the dark striped pufferfish embryo. After the actual expected incubation temperature T d e is determined, the temperature is used as a target value to adjust the temperature of the water in the buffer tank.

[0069] S30, obtain the current actual incubation water temperature, and use the fuzzy control system to adjust the current actual incubation water temperature to approach the actual expected incubation temperature.

[0070] In the embodiments of the present application, the current actual incubation water temperature can be obtained by temperature sensor acquisition.

[0071] In an embodiment of the present invention, step S30, obtaining the current actual incubation water temperature and adjusting it towards the actual desired incubation temperature using a fuzzy control system, includes: the fuzzy control system includes a temperature sensor, an input module, a PID controller, and a fuzzy controller.

[0072] S301. Obtain the current actual incubation water temperature. Compared with the actual expected incubation temperature T d e The error value e and the rate of change of error ec;

[0073] S302, The current actual incubation water temperature The error value e and the rate of change ec are quantized and input to the PID controller. The fuzzy controller then infers the fuzzy quantity. , and Three outputs;

[0074] S303, Based on the fuzzy quantity , and For PID controllers respectively , and Adjustments are made to optimize the performance of the PID controller, thereby improving the accuracy of temperature regulation while reducing overshoot.

[0075] S304. The adjusted fuzzy controller controls and adjusts the water temperature in the incubation tank.

[0076] Specifically, it can be expressed by the following formula:

[0077]

[0078]

[0079]

[0080] In the formula , and These are the basic values ​​of the tuning parameters in a conventional PID controller; fuzzy quantity , and Parameters , and The correction value.

[0081] In this embodiment of the invention, the fuzzy universe of discourse for the two inputs e and ec is set to [-3, 3], and the three outputs correspond to the fuzzy quantities Δk.p , Δk i and Δk d The fuzzy domain of is set to [-3, 3], and the input and output both use trimf type membership functions. The fuzzy domain corresponds to seven fuzzy language variables {NB (negative big), NM (negative medium), NS (negative small), ZO (zero), PS (positive small), PM (positive medium), PB (positive big)}. The fuzzy control rules are shown in Table 2 below, the Mamdani method is used for fuzzy reasoning, and the centroid method is used for defuzzification. The simulation models of the two controllers are established as shown in Figure 3

[0082] Fuzzy control rule table

[0083] Table 2 Fuzzy control rules table

[0084]

[0085] The present application is mainly applied to winter environment, and the actual temperature is lower than the target temperature more often, so the temperature rising process is mainly considered, which is realized by adding 39℃ underground water, and the temperature is lowered by adding water with lower temperature. When the water temperature in the breeding pond is lower than the set temperature range, in order to make the water temperature rise to the set value range, the temperature adjusting water is put into the buffer tank by opening the electromagnetic valve, and the specific temperature adjusting process is as shown in Figure 3

[0086] The fuzzy control algorithm is based on fuzzy set theory, fuzzy variables and fuzzy logic reasoning, and combines systematic theory, practical application experience of experts and conventional PID.

[0087] The fish embryo hatching fuzzy PID temperature regulation algorithm based on effective accumulated temperature model for factory hatching of oriental dark stripe porpoise embryos realizes intelligent regulation and control of water temperature for factory hatching of embryos.

[0088] It should be understood that although the above steps are described in a certain order, these steps are not necessarily executed in the above order. Unless explicitly stated herein, the execution of these steps is not strictly limited in order, and these steps can be executed in other orders. Moreover, part of the steps of the present embodiment can include multiple steps or multiple stages, which are not necessarily executed at the same time, but can be executed at different times, and the execution order of these steps or stages is not necessarily sequential, but can be executed alternately or alternately with at least part of other steps or steps or stages in other steps. ​​

[0089] In one embodiment, referring to Figure 4 In an embodiment of the present application, a dark marbled puffer embryo hatching cycle control system based on an effective accumulated temperature model is also provided, which comprises a target hatching cycle acquisition module 100, an actual desired hatching temperature acquisition module 200 and a temperature adjustment module 300.

[0090] The target hatching cycle acquisition module 100 is configured to acquire a target hatching cycle based on the hatching environment temperature.

[0091] The actual desired hatching temperature acquisition module 200 is configured to acquire an actual desired hatching temperature of each day according to the effective accumulated temperature model and the target hatching cycle.

[0092] The temperature adjustment module 300 is configured to acquire a current actual hatching water temperature and adjust the current actual hatching water temperature to be close to the actual desired hatching temperature by using a fuzzy control system.

[0093] In one embodiment, an electronic device is also provided in an embodiment of the present application, which comprises a processor, a communication interface, a memory and a communication bus, wherein the processor, the communication interface and the memory complete mutual communication through the communication bus.

[0094] The memory is configured to store a computer program.

[0095] The processor is configured to execute the computer program stored on the memory to execute the dark marbled puffer embryo hatching cycle control method based on the effective accumulated temperature model, and the processor executes instructions to implement the steps in the above method embodiments.

[0096] The communication bus mentioned above can be a peripheral component interconnect (PCI) bus or an extended industry standard architecture (EISA) bus, etc. The communication bus can be divided into an address bus, a data bus, a control bus, etc. For the convenience of representation, only one thick line is shown in the figure, but it does not mean that there is only one bus or only one type of bus.

[0097] The communication interface is configured to communicate between the terminal and other devices.

[0098] The memory can include a random access memory (RAM) and can also include a non-volatile memory, such as at least one disk memory. Optionally, the memory can also be at least one storage device located away from the aforementioned processor.

[0099] The processor described above can be a general processor, including a central processing unit (CPU), a network processor (NP), etc.; can also be a digital signal processor (DSP), an application specific integrated circuit (ASIC), a field-programmable gate array (FPGA) or other programmable logic device, a discrete gate or transistor logic device, a discrete hardware component.

[0100] The electronic device includes a user device and a network device. The user device includes, but is not limited to, a computer, a smart phone, a PDA, etc.; the network device includes, but is not limited to, a single network server, a server group composed of multiple network servers, or a cloud composed of a large number of computers or network servers based on cloud computing. Cloud computing is a kind of distributed computing, which is composed of a super virtual computer formed by a group of loosely coupled computer clusters. The electronic device can be operated alone to realize the present application, or can be connected to a network and interact with other electronic devices in the network to realize the present application. The network in which the electronic device is located includes, but is not limited to, the Internet, a wide area network, a metropolitan area network, a local area network, a VPN network, etc.

[0101] It should also be understood that the term "and / or" used in the specification and the appended claims of the present application means any combination of one or more of the associated listed items and all possible combinations, and includes these combinations.

[0102] In one embodiment of the present application, a storage medium having a computer program stored thereon is also provided, and the computer program is executed by a processor to implement the steps in the above method embodiments.

[0103] Those of ordinary skill in the art can understand that all or part of the processes in the above embodiments can be completed by a computer program instructing related hardware, and the computer program can be stored in a non-volatile computer readable storage medium. When the computer program is executed, it can include the processes of the above embodiments. Any reference to memory, storage, database or other medium used in the embodiments provided by the present application can include at least one of non-volatile and volatile memory.

[0104] It should be understood that, as used herein, the singular form "a" is intended to include the plural form as well, unless the context clearly supports an exception. It should also be understood that, as used herein, "and / or" refers to any and all possible combinations of one or more of the associatedly listed items. The embodiment numbers disclosed above are for descriptive purposes only and do not represent the superiority or inferiority of the embodiments.

[0105] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of the invention (including the claims) is limited to these examples. Within the framework of the invention, technical features of the above embodiments or different embodiments can be combined, and many other variations of different aspects of the invention exist, which are not provided in the details for the sake of brevity. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the invention should be included within the protection scope of the invention.

Claims

1. A method for regulating the hatching period of the Japanese pufferfish (Takifugu obscurus) embryo based on the effective accumulated temperature model, characterized by, The method comprises: based on the incubation environment temperature, to obtain the target incubation period; according to the effective accumulated temperature model and the target incubation period, to obtain the actual expected incubation temperature of each day; obtain the current actual incubation water temperature, and adjust the current actual incubation water temperature to the actual expected incubation temperature of the day by using the fuzzy control system.

2. The method for regulating the embryo hatching cycle of *Pufferfish obscurus* based on an effective accumulated temperature model as described in claim 1, characterized in that... The target incubation period is obtained by the following formula: N = 78.905 / (T-7.6033) In the formula, N is the target incubation period; T is the incubation environment temperature.

3. The method for regulating the embryo hatching cycle of *Pufferfish obscurus* based on an effective accumulated temperature model as described in claim 2, characterized in that... The actual expected incubation temperature of the nth day is obtained by the following formula: wherein Tn is the actual expected hatch temperature for day n; T iavg T is the actual average temperature for day i; T zero Biozero; T count Total effective accumulated temperature.

4. The method for controlling the hatching period of the Pterois lunulata embryo based on the effective accumulated temperature model according to claim 3, wherein the accumulated temperature is calculated by the following formula: T = ∑(Tmax - Tn) where T is the accumulated temperature, Tmax is the maximum temperature of the day, and Tn is the average temperature of the day. The total amount of effective accumulated temperature is obtained by the following formula: In the formula, T ieff is the effective accumulated temperature on the i-th day in the current growth stage.

5. The method for regulating the embryo hatching cycle of *Pufferfish obscurus* based on an effective accumulated temperature model as described in claim 1, characterized in that... obtain the current actual incubation water temperature, and adjust the current actual incubation water temperature to the actual expected incubation temperature by using the fuzzy control system, comprising: obtaining a current actual incubation water temperature T now an error value e and a rate of change of the error ec between the actual desired incubation temperature T d e ​ The current actual incubation water temperature T now , the error value e and the change rate ec are quantified and input into the fuzzy controller to obtain the fuzzy quantity Δk through reasoning p , Δk i and Δk d three outputs; According to the blur amount Δk p , Δk i , and Δk d , the k p , k i , and k d of the PID controller are adjusted, respectively; The adjusted PID controller controls and adjusts the water temperature of the incubation pool.

6. The method for controlling the period of hatching of the Pterophlym pterosus Linnaeus embryo based on the effective accumulated temperature model according to claim 5, wherein, The fuzzy domain of error value e and change rate ec is set as [-3, 3], and the fuzzy quantity Δk p The fuzzy domain of error value e and change rate ec is set as [-3, 3], and the fuzzy quantity Δk i The fuzzy domain of error value e and change rate ec is set as [-3, 3], and the fuzzy quantity Δk d The fuzzy domain of error value e and change rate ec is set as [-3, 3], and the fuzzy quantity Δk 7. A dark pattern of pufferfish embryo hatching cycle regulation system based on effective accumulated temperature model, characterized in that, The system comprises a target incubation period acquisition module, an actual expected incubation temperature acquisition module and a temperature adjustment module; The target incubation period acquisition module is used to obtain the target incubation period based on the incubation environment temperature; The actual expected incubation temperature acquisition module is used to obtain the actual expected incubation temperature of each day according to the effective accumulated temperature model and the target incubation period; The temperature adjustment module is used to obtain the current actual incubation water temperature, and adjust the current actual incubation water temperature to the actual expected incubation temperature by using the fuzzy control system. 8.An electronic device comprising a memory and a processor, wherein the memory stores a computer program, and the processor loads and executes the computer program to implement the steps of the incubation period regulation method for T. obscurus embryos based on the effective accumulated temperature model according to any one of claims 1-6. 9.A storage medium storing a computer program, wherein the computer program is loaded and executed by a processor to implement the steps of the incubation period regulation method for T. obscurus embryos based on the effective accumulated temperature model according to any one of claims 1-6.

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