A micro analyzer based on self-compensating near-infrared SPR effect

Abstract

The present invention discloses a micro analyzer based on self-compensation near-infrared SPR effect, and relates to the field of SPR sensing technology. The micro analyzer includes a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module; the laser emission module emits C-band laser; the light intensity self-compensation module divides the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect; the multi-channel detection module simultaneously detects a variety of samples to be tested; the SPR excitation module generates multi-channel reflected light with SPR effect; the multi-channel photoelectric detection module detects the light intensity signal of s-polarized light and multi-channel reflected light; the signal processing module determines the refractive index and concentration of the sample to be tested according to the light intensity signal; the result display module displays the refractive index and concentration. The present invention can reduce the cost and volume of the instrument while achieving high sensitivity, high precision and rapid detection.

Description

Technical Field

[0001] The invention relates to the field of SPR sensing technology, in particular to a micro analyzer based on self-compensation near-infrared SPR effect. Background Art

[0002] SPR (Surface Plasmon Resonance) technology is an important bioanalysis technology that can detect the interaction between biomolecules in real time. SPR technology is based on the adsorption of biomolecules on metal surfaces. It uses the change in reflected light intensity caused by surface plasmon waves generated by laser irradiation to detect the interaction between biomolecules. It is widely used in drug screening, biosensing and biomedical research.

[0003] Currently, high-performance SPR analyzers on the market are generally large in size and high in cost, which limits the popularization of SPR analyzers. Summary of the invention

[0004] The object of the present invention is to provide a micro analyzer based on the self-compensating near-infrared SPR effect, so as to reduce the cost and volume of the instrument while achieving high sensitivity, high precision and rapid detection.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] A micro analyzer based on self-compensation near-infrared SPR effect, comprising: a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module;

[0007] The laser emission module is used to emit C-band laser, and the C-band laser is incident on the light intensity self-compensation module;

[0008] The light intensity self-compensation module is used to divide the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect, and the p-polarized light is incident on the SPR excitation module, and the s-polarized light is incident on the multi-path photoelectric detection module;

[0009] The multi-channel detection module includes multiple sample test channels and non-specific reference channels for simultaneously detecting multiple samples to be tested;

[0010] The SPR excitation module uses p-polarized light to excite the SPR effect of the sample to be tested and generates multi-channel reflected light with SPR effect, and the multi-channel reflected light is incident on the multi-channel photoelectric detection module;

[0011] The multi-channel photoelectric detection module is used to detect the light intensity signals of the s-polarized light and the multi-channel reflected light and send them to the signal processing module;

[0012] The signal processing module is used to determine the refractive index and concentration of the sample to be tested according to the light intensity signal and send them to the result display module;

[0013] The result display module is used to display the refractive index and concentration.

[0014] Optionally, the laser emission module includes: a fiber laser transmitter, a transmission fiber, a C-band laser diode, an incident light fiber collimator, and a collimator bracket;

[0015] The fiber laser transmitter is connected to the C-band laser diode via the transmission optical fiber; the fiber laser transmitter is used to generate laser light and is incident on the C-band laser diode via the transmission optical fiber, and the C-band laser diode generates C-band laser light;

[0016] The incident light fiber collimator is mounted on the collimator bracket; the collimator bracket is used to fix the incident light fiber collimator on the optical path of the C-band laser incident on the light intensity self-compensation module, and the incident light fiber collimator is used to collimate the C-band laser and expand the beam diameter.

[0017] Optionally, the light intensity self-compensation module includes: a polarization beam splitter prism and a polarization beam splitter prism bracket;

[0018] The polarization beam splitter prism is installed in the polarization beam splitter prism bracket; the polarization beam splitter prism bracket is connected with the collimator bracket through a thread;

[0019] The polarization beam splitter prism splits the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect.

[0020] Optionally, the SPR excitation module comprises: a semi-cylindrical prism bracket, a semi-cylindrical prism and a sensor chip; the sensor chip comprises a sensor film and a glass sheet;

[0021] The semi-cylindrical prism is installed in the semi-cylindrical prism bracket; the semi-cylindrical prism bracket is cooperatively connected with the polarization beam splitter prism bracket;

[0022] The glass sheet is located on the plane of the semi-cylindrical prism, and the semi-cylindrical prism and the glass sheet are coupled via a refractive index matching liquid; and the sensing film is arranged on the glass sheet.

[0023] Optionally, the p-polarized light is incident on the sensor chip at a fixed angle, and the fixed angle is 62.77-62.8°.

[0024] Optionally, the multi-channel detection module comprises: a cover plate, a hose and a hose plug arranged on the housing;

[0025] The cover plate is located on the top of the housing, and a plurality of partitions are arranged on the cover plate; the hose plug is provided with threads, and the hose is fixed to each partition of the cover plate through the hose plug;

[0026] The sensor chip is located directly below the cover plate, and each partition of the cover plate corresponds to multiple channels on the sensor chip, including multiple sample test channels and non-specific reference channels; different specific detection molecules are introduced into each sample test channel through a corresponding hose to modify the sensor chip; the non-specific reference channel corresponds to a sensor membrane that is not modified with specific detection molecules.

[0027] Optionally, the multi-channel photoelectric detection module includes: a light intensity reference channel detector, multiple sample test channel detectors and a non-specific reference channel detector;

[0028] The light intensity reference channel detector is installed in the polarization beam splitter prism bracket and is located on the outgoing light path of the s-polarized light, and is used to detect the light intensity signal of the s-polarized light;

[0029] The multiple sample test channel detectors and the non-specific reference channel detector are installed on the semi-cylindrical prism bracket and are located on the outgoing light path of the multi-channel reflected light.

[0030] Optionally, the signal processing module includes: a multi-channel signal converter, a single-chip microcomputer expansion board and a single-chip microcomputer connected in sequence;

[0031] The multi-channel signal converter is connected to the multi-channel photoelectric detection module and is used to convert the multi-channel light intensity signals into multi-channel electrical signals; the multi-channel electrical signals are transmitted to the single-chip microcomputer via the single-chip microcomputer expansion board;

[0032] The single chip microcomputer is used to calculate the refractive index and concentration of various samples to be tested according to multi-channel electrical signals.

[0033] Optionally, the result display module includes: a display screen;

[0034] The display screen is located on the top of the housing and is connected to the single chip microcomputer, and is used for displaying the refractive index and concentration of a variety of samples to be tested.

[0035] Optionally, the micro analyzer further comprises: a power supply device, a charging port and a power switch;

[0036] The power supply device is located inside the shell; the charging port and the power switch are located on one side of the shell; the power supply device is connected to an external power source through the charging port for charging; the power supply device is respectively connected to the laser emission module, the multi-channel photoelectric detection module, the signal processing module and the result display module through the power switch for power supply.

[0037] According to the specific embodiments provided by the present invention, the present invention discloses the following technical effects:

[0038] The invention provides a micro analyzer based on self-compensation near-infrared SPR effect, comprising a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module. Among them, the laser emission module is used to emit C-band laser and incident it to the light intensity self-compensation module; the light intensity self-compensation module is used to divide the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect, and the p-polarized light is incident on the SPR excitation module, and the s-polarized light is incident on the multi-channel photoelectric detection module; the multi-channel detection module includes multiple sample test channels and non-specific reference channels, which are used to simultaneously detect multiple samples to be tested; the SPR excitation module uses p-polarized light to excite the SPR effect of the sample to be tested and generates multi-channel reflected light with the SPR effect, and the multi-channel reflected light is incident on the multi-channel photoelectric detection module; the multi-channel photoelectric detection module is used to detect the light intensity signals of the s-polarized light and the multi-channel reflected light and send them to the signal processing module; the signal processing module is used to determine the refractive index and concentration of the sample to be tested according to the light intensity signal and send them to the result display module; the result display module is used to display the refractive index and concentration. The present invention provides a micro analyzer based on self-compensation near-infrared SPR effect, which realizes the detection of the concentration of a sample to be tested based on the near-infrared SPR effect. Compared with the existing detection device using a spectrometer, the device volume is reduced on the basis of ensuring the sensing performance; in addition, the present invention provides multiple test channels, which can detect multiple objects to be tested at the same time; and the self-compensation module and reference channel provided in the present invention greatly improve the sensitivity and stability of the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0040] Figure 1 A schematic diagram of the overall structure of the micro analyzer provided by the present invention;

[0041] Figure 2 Schematic diagrams of the front and back of the sensing detection area of ​​the micro analyzer provided by the present invention and an enlarged schematic diagram of the sensing membrane area;

[0042] Figure 3 A schematic diagram of the external structure of the micro analyzer provided by the present invention;

[0043] Figure 4 A schematic diagram of the shell dimensions of the micro analyzer provided by the present invention. DETAILED DESCRIPTION

[0044] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] Since the high-performance SPR analyzers currently on the market are generally large in size and high in cost, the popularity of SPR analyzers is limited to a certain extent. Therefore, it is of great significance to research and develop a SPR analyzer that takes into account miniaturization and high sensitivity, and can effectively reduce the cost and volume of the instrument while achieving high sensitivity, high precision and rapid detection. The object of the present invention is to provide a micro analyzer based on self-compensating near-infrared SPR effect, so as to reduce the cost and volume of the instrument while achieving high sensitivity, high precision and rapid detection. The micro analyzer of the present invention can be used for rapid and accurate detection of biomolecules, with the advantages of low cost, miniaturization, high precision, and ease of use.

[0046] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0047] Figure 1 The overall structural diagram of a micro analyzer based on self-compensation near-infrared SPR effect provided by the present invention is shown in FIG. Figure 2 The front and back schematic diagrams of the sensing detection area of ​​the micro analyzer provided by the present invention and the enlarged schematic diagram of the sensing membrane area are as follows: Figure 1 and Figure 2 As shown, the present invention provides a micro analyzer based on self-compensation near-infrared SPR effect, including: a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module.

[0048] The laser emission module is used to emit C-band laser, and to make the C-band laser incident on the light intensity self-compensation module.

[0049] Specifically, the laser emission module includes: a fiber laser emitter 1 , a transmission fiber 22 , a C-band laser diode 2 , an incident light fiber collimator 3 and a collimator bracket 19 .

[0050] like Figure 1 and Figure 2 As shown, the fiber laser transmitter 1 is connected to the C-band laser diode 2 via the transmission fiber 22. The fiber laser transmitter 1 supplies power to the C-band laser diode 2 to generate laser light and adjust the laser intensity. The generated light is incident on the C-band laser diode 2 via the transmission fiber 22, and the C-band laser diode 2 generates C-band laser light.

[0051] The incident light fiber collimator 3 is installed on the collimator bracket 19; the incident light fiber collimator 3 is connected to the collimator bracket 19 through threads, and the collimator bracket 19 is used to fix the incident light fiber collimator 3 on the optical path from the C-band laser to the light intensity self-compensation module, and is used to collimate the C-band laser and expand the beam diameter so that the C-band laser can cover the entire sensor chip.

[0052] The light intensity self-compensation module is used to divide the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect, and to input the p-polarized light into the SPR excitation module and the s-polarized light into the multi-path photoelectric detection module.

[0053] Specifically, the light intensity self-compensation module comprises: a polarization beam splitter prism 4 and a polarization beam splitter prism bracket 18. The polarization beam splitter prism 4 is installed in the polarization beam splitter prism bracket 18; the polarization beam splitter prism bracket 18 is connected with the collimator bracket 19 through threads.

[0054] The polarization beam splitter 4 divides the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect. Among them, the s-polarized light provides a reference signal for the detection signal to correct the error caused by light source fluctuations, etc. By referring to the light intensity, fluctuation and other signals of the s-polarized light, the p-polarized light that excites the SPR effect is processed accordingly, which can reduce the error of the molecular detection signal caused by light source noise, chip preparation process and non-specific adsorption.

[0055] Specifically, the multi-channel detection module includes multiple sample test channels and non-specific reference channels, which are used to simultaneously detect multiple samples to be tested. The sensing channels of the present invention are divided into two types: one is a multi-channel sample test channel, and the other is a non-specific reference channel. In order to simultaneously detect multiple samples to be tested, a multi-channel sample test channel needs to be arranged. Before use, different specific detection molecules are introduced into each sample test channel to modify the sensor chip; and the non-specific reference channel corresponds to a sensor chip with a metal sensor membrane that is not modified with a specific detection molecule membrane. Except for the specific detection molecule membrane, the material, gold film thickness and other parameters of the sensor chips of the multi-channel sample test channel and the non-specific reference channel are the same.

[0056] See also Figure 1 and Figure 3 The multi-channel detection module includes: a cover plate 17, a hose 24 and a hose plug 25 arranged on the housing 13. The cover plate 17 is located on the top of the housing 13, and a plurality of partitions ( Figure 3 3 partitions are shown in FIG. 1 ). The partitions on the cover plate 17 divide the sensing membrane 7 into a plurality of sample test channels and a non-specific reference channel for simultaneously detecting a plurality of samples to be tested and providing a reference signal for the detection signal to correct the result.

[0057] The hose plug 25 is provided with threads, and the hose 24 is fixed to each partition of the cover plate 17 through the hose plug 25. The sensor chip is located directly below the cover plate 17, and each partition of the cover plate 17 corresponds to multiple channels on the sensor chip, including multiple sample test channels and non-specific reference channels; each sample test channel is passed through a hose 24 at a corresponding position to introduce different specific detection molecules to modify the sensor chip; the non-specific reference channel corresponds to a sensor membrane that is not modified with specific detection molecules.

[0058] Specifically, the SPR excitation module utilizes p-polarized light to excite the SPR effect of the sample to be tested and generates multi-channel reflected light with the SPR effect, and the multi-channel reflected light is incident on the multi-channel photoelectric detection module.

[0059] See also Figure 1 and Figure 2 The SPR excitation module includes a semi-cylindrical prism support 23, a semi-cylindrical prism 5 and a sensor chip. The sensor chip includes a sensor film 7 and a glass sheet 6. In a specific embodiment, the semi-cylindrical prism 5 is preferably a K9 semi-cylindrical prism, and the glass sheet 6 is a K9 glass sheet.

[0060] The semi-cylindrical prism 5 is installed in the semi-cylindrical prism bracket 23; the semi-cylindrical prism bracket 23 is connected with the polarization splitter prism bracket 18. The glass sheet 6 is located on the plane of the semi-cylindrical prism 5, and the semi-cylindrical prism 5 and the glass sheet 6 are coupled by a refractive index matching liquid; the sensing film 7 is arranged on the glass sheet 6. The glass sheet 6 is detachable, and the material and thickness of the sensing film 7 are determined by physical and chemical methods according to the type of the sample to be tested, and the sensing film 7 is modified on the glass sheet 6. Among them, for different detection substances, the corresponding biomolecular film system is modified, and different samples to be tested are detected by replacing the corresponding sensing film 7 when the top cover plate 17 of the housing 13 is opened. For example, a gold film with a thickness of 50nm is a sensing film 7 commonly used in the detection device.

[0061] The p-polarized light that can excite the SPR effect is incident on the semi-cylindrical prism 5 and the sensor chip in turn. The p-polarized light covers the sensor chips of multiple channels and excites the near-infrared SPR effect, so that the SPR evanescent field is radiated to the sample to be tested in the near field of the sensor chip. Subsequently, the multi-channel reflected light carrying the SPR absorption characteristics and the refractive index information of the sample to be tested passes through the sensor chip and the semi-cylindrical prism 5 in turn and is detected by multiple InGaAs photodetectors.

[0062] As an embodiment, p-polarized light is incident on the sensor chip at a fixed angle, wherein the fixed angle is 62.77°-62.8°. The incident angle of p-polarized light determines the size of the resonance wavelength. Setting the incident angle to 62.77°-62.8° allows the sensitivity to be optimal when the resonance wavelength is slightly larger than the wavelength of the fiber laser transmitter.

[0063] Specifically, the multi-channel photoelectric detection module is used to detect the light intensity signals of the s-polarized light and the multi-channel reflected light and send them to the signal processing module.

[0064] The multi-channel photoelectric detection module of the present invention can detect multi-channel light intensity signals, including the light intensity signal of the light intensity self-compensation module and the multi-channel light intensity signal of the SPR excitation module. The multi-channel photoelectric detection module includes multiple InGaAs photodetectors, each of which includes a light intensity reference channel detector 801, multiple sample test channel detectors, and a non-specific reference channel detector 804. Among them, the light intensity reference channel detector 801 is installed in the polarization beam splitter prism bracket 18 and is located on the outgoing light path of s-polarized light, and is used to detect the light intensity signal of s-polarized light. Multiple sample test channel detectors and non-specific reference channel detector 804 are installed on the semi-cylindrical prism bracket 23 and are located on the outgoing light path of multi-channel reflected light.

[0065] As a specific embodiment, the partition on the cover plate 17 divides the sensor membrane 7 into two sample test channels (sample A test channel and sample B test channel) and a non-specific reference channel, a total of three channels. The present invention first modifies the non-specific sensor membrane on the glass sheet 6: the class A specific detection molecule is passed into the sample A test channel, the class B specific detection molecule membrane is passed into the sample B test channel, and the non-specific reference channel corresponds to the sensor membrane without the specific detection molecule membrane. If a variety of samples A and B to be tested are dissolved in the same sample to be tested, the samples to be tested can be passed into all channels at the same time. The sample A test channel detector 802, the sample B test channel detector 803 and the non-specific reference channel detector 804 are correspondingly arranged at the sample A test channel, the sample B test channel and the non-specific reference channel, which are used to detect the light intensity signal of the reflected light of each channel and compare it. The light intensity signal obtained by the non-specific reference channel detector 804 is used as a control signal, and the concentrations of the sample A to be tested and the sample B to be tested can be obtained.

[0066] Specifically, the signal processing module is used to determine the refractive index and concentration of the sample to be tested according to the light intensity signal and send them to the result display module.

[0067] See also Figure 1 and Figure 2 , the signal processing module includes a multi-channel signal converter 9, a single-chip microcomputer expansion board 20 and a single-chip microcomputer 10 connected in sequence. Among them, the multi-channel signal converter 9 is connected to the multi-channel photoelectric detection module, that is, the multi-channel signal converter 9 is connected to multiple indium gallium arsenide photodetectors through wires, and is used to convert multiple light intensity signals into multiple electrical signals. The multiple electrical signals are transmitted to the single-chip microcomputer 10 via the single-chip microcomputer expansion board 20, that is, the multi-channel signal converter 9 is connected to the single-chip microcomputer 10 and the single-chip microcomputer expansion board 20 through wires. Among them, the multi-channel signal converter 9 converts the multiple light intensity signals into multiple current signals, the single-chip microcomputer expansion board 20 converts the current signals into voltage signals, and transfers the voltage signals to the single-chip microcomputer 10; the single-chip microcomputer 10 is connected to the single-chip microcomputer expansion board 20 through pins, and the single-chip microcomputer expansion board 20 is connected to the multi-channel signal converter 9 through wires, and receives the light intensity information collected by multiple indium gallium arsenide photodetectors in real time; and the single-chip microcomputer 10 is used to determine the corresponding light intensity information according to the multi-channel voltage signals, and calculate the refractive index and concentration of multiple samples to be tested through the light intensity information.

[0068] Specifically, the result display module is used to display the refractive index and concentration.

[0069] Figure 3 The schematic diagram of the external structure of the micro analyzer provided by the present invention is as follows: Figure 3As shown, the result display module includes a display screen 11, wherein the display screen 11 is a serial port liquid crystal screen. The display screen 11 is located on the top of the housing 13 and is connected to the single chip computer 10, and is used to display the refractive index and concentration of a plurality of samples to be tested.

[0070] like Figure 3 As shown, the outer surface of the shell 13 of a micro analyzer based on self-compensating near-infrared SPR effect provided by the present invention is provided with heat dissipation holes 14, a power switch 15, a charging port 16, a USB port 21, a cover plate 17 and a display screen 11, wherein the USB port 21, the cover plate 17 and the display screen 11 are located at the top of the shell 13, the heat dissipation holes 14 are located on the front side of the shell 13, and the charging port 16 and the power switch 15 are located on one side of the shell 13.

[0071] The micro analyzer is internally provided with a fiber laser transmitter 1, a power supply device 12, a multi-channel signal converter 9, and a single-chip expansion board 20, which are respectively connected and fixed to the housing 13 by screws. The power supply device 12 is connected to an external power source through a charging port 16 for charging; and the power supply device 12 is respectively connected to the laser emission module, the multi-channel photoelectric detection module, the signal processing module, and the result display module through a power switch 15 for power supply. The single-chip computer 10 transmits the calculated refractive index and concentration data of the sample to be tested to the display screen 11, and can save the data to the user's storage device through the USB port 21.

[0072] As an embodiment, the present invention uses a connecting bracket to fix various optical components, uses a transmission optical fiber 22 to connect the optical path, and is integrated into the internal structure of the instrument.

[0073] Figure 4 The schematic diagram of the shell size of the micro analyzer provided by the present invention is as follows: Figure 4 As shown, the housing 13 of the micro analyzer is a=12 cm long, b=7 cm wide, and c=8 cm high. Compared with the existing high-precision detection instruments, the present invention effectively reduces the instrument volume and achieves both high precision and miniaturization.

[0074] The micro analyzer based on the self-compensation near-infrared SPR effect provided by the present invention is used in the following specific steps when testing a sample to be tested:

[0075] 1) Turn on the power switch 15 to start the micro analyzer.

[0076] 2) Open the cover plate 17 on the top of the housing 13 , drop a drop of refractive index matching liquid on the semi-cylindrical prism 5 , and place the glass sheet 6 coated with a non-specific sensing film on the semi-cylindrical prism 5 .

[0077] 3) Close the cover plate 17 on the top of the housing 13 , and pass the specific detection substance corresponding to the sample to be tested into the sensor chip through the hose 24 on the cover plate 17 .

[0078] 4) Drop the sample to be tested onto the sensor chip, or pass the sample to be tested onto the sensor chip through the hose 24.

[0079] 5) Click the confirmation button on the display screen 11, and the display screen 11 displays the refractive index and concentration of the sample to be tested.

[0080] 6) Insert the USB disk into the USB port 21 to store data.

[0081] 7) After use, turn off the power switch 15 and take out the sensor chip for next use.

[0082] In summary, compared with existing detection instruments on the market, the micro analyzer provided by the present invention has the following advantages:

[0083] (1) Compared with the detection instruments with higher detection accuracy in the prior art, the micro-analyzer of the present invention uses a near-infrared light source. Since the laser wavelength used is in the C band to stimulate the near-infrared SPR effect, the detection sensitivity is improved, the detection accuracy is greatly enhanced, and online real-time detection is realized.

[0084] (2) The micro analyzer of the present invention adds a polarization beam splitter prism 4, which greatly reduces the half-peak width of the incident light and improves the quality factor of the sensor.

[0085] (3) The micro analyzer of the present invention adopts an InGaAs photodetector instead of a spectrometer, adopts a single-chip microcomputer 10 instead of a computer, adopts a small display screen 11 instead of a traditional display screen, and adopts a charging module instead of an online power supply, thereby reducing the volume of optical devices, achieving portability and miniaturization of the SPR sensor device, and greatly reducing the detection cost.

[0086] (4) Compared with the intensity modulated SPR device in the prior art, the micro analyzer of the present invention adopts a self-compensation module and a multi-channel detection module to achieve self-compensation of light source stability, thereby improving the sensitivity of detection.

[0087] (5) The micro analyzer of the present invention uses a multi-channel detection module to simultaneously detect different samples to be tested, and the light intensity signal of the reference channel can be used as a control signal to correct signal errors, saving time costs and greatly enhancing the stability and sensitivity of the intensity modulated SPR device.

[0088] (6) The micro-analyzer of the present invention adopts a semi-cylindrical prism 5, which reduces the volume of the instrument and the cost required for correcting the incident light angle.

[0089] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0090] The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A micro analyzer based on self-compensating near-infrared SPR effect, characterized in that: include: Laser emission module, light intensity self-compensation module, multi-channel detection module, SPR excitation module, multi-channel photoelectric detection module, signal processing module and result display module; The laser emission module is used to emit C-band laser, and the C-band laser is incident on the light intensity self-compensation module; The light intensity self-compensation module is used to divide the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect, and the p-polarized light is incident on the SPR excitation module, and the s-polarized light is incident on the multi-path photoelectric detection module; The multi-channel detection module includes multiple sample test channels and non-specific reference channels for simultaneously detecting multiple samples to be tested; The SPR excitation module uses p-polarized light to excite the SPR effect of the sample to be tested and generates multi-channel reflected light with SPR effect, and the multi-channel reflected light is incident on the multi-channel photoelectric detection module; The multi-channel photoelectric detection module is used to detect the light intensity signals of the s-polarized light and the multi-channel reflected light and send them to the signal processing module; The signal processing module is used to determine the refractive index and concentration of the sample to be tested according to the light intensity signal and send them to the result display module; The result display module is used to display the refractive index and concentration.

2. The micro analyzer according to claim 1, characterized in that: The laser emission module comprises: an incident light fiber collimator and a collimator bracket; The incident light fiber collimator is mounted on the collimator bracket; the collimator bracket is used to fix the incident light fiber collimator on the optical path of the C-band laser incident on the light intensity self-compensation module, and the incident light fiber collimator is used to collimate the C-band laser and expand the beam diameter.

3. The micro analyzer according to claim 2, characterized in that: The light intensity self-compensation module comprises: a polarization beam splitter prism and a polarization beam splitter prism bracket; The polarization beam splitter prism is installed in the polarization beam splitter prism bracket; the polarization beam splitter prism bracket is connected with the collimator bracket through a thread; The polarization beam splitter prism splits the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect.

4. The micro analyzer according to claim 3, characterized in that: The SPR excitation module comprises: a semi-cylindrical prism bracket, a semi-cylindrical prism and a sensor chip; the sensor chip comprises a sensor film and a glass sheet; The semi-cylindrical prism is installed in the semi-cylindrical prism bracket; the semi-cylindrical prism bracket is cooperatively connected with the polarization beam splitter prism bracket; The glass sheet is located on the plane of the semi-cylindrical prism, and the semi-cylindrical prism and the glass sheet are coupled via a refractive index matching liquid; and the sensing film is arranged on the glass sheet.

5. The micro analyzer according to claim 4, characterized in that: The p-polarized light is incident on the sensor chip at a fixed angle, and the fixed angle is 62.77°-62.8°.

6. The micro analyzer according to claim 4, characterized in that: The multi-channel detection module comprises: a cover plate, a hose and a hose plug arranged on the housing; The cover plate is located on the top of the housing, and a plurality of partitions are arranged on the cover plate; the hose plug is provided with threads, and the hose is fixed to each partition of the cover plate through the hose plug; The sensor chip is located directly below the cover plate, and each partition of the cover plate corresponds to multiple channels on the sensor chip, including multiple sample test channels and non-specific reference channels; different specific detection molecules are introduced into each sample test channel through a corresponding hose to modify the sensor chip; the non-specific reference channel corresponds to a sensor membrane that is not modified with specific detection molecules.

7. The micro analyzer according to claim 6, characterized in that: The multi-channel photoelectric detection module includes: a light intensity reference channel detector, multiple sample test channel detectors and a non-specific reference channel detector; The light intensity reference channel detector is installed in the polarization beam splitter prism bracket and is located on the outgoing light path of the s-polarized light, and is used to detect the light intensity signal of the s-polarized light; The multiple sample test channel detectors and the non-specific reference channel detector are installed on the semi-cylindrical prism bracket and are located on the outgoing light path of the multi-channel reflected light.

8. The micro analyzer according to claim 7, characterized in that: The signal processing module includes: a multi-channel signal converter, a single-chip microcomputer expansion board and a single-chip microcomputer connected in sequence; The multi-channel signal converter is connected to the multi-channel photoelectric detection module and is used to convert the multi-channel light intensity signals into multi-channel electrical signals; the multi-channel electrical signals are transmitted to the single-chip microcomputer via the single-chip microcomputer expansion board; The single chip microcomputer is used to calculate the refractive index and concentration of various samples to be tested according to multi-channel electrical signals.

9. The micro analyzer according to claim 8, characterized in that: The result display module comprises: a display screen; The display screen is located on the top of the housing and is connected to the single chip microcomputer, and is used for displaying the refractive index and concentration of a variety of samples to be tested.

10. The micro analyzer according to claim 9, characterized in that: Also includes: Power supply unit, charging port and power switch; The power supply device is located inside the housing; The charging port and the power switch are located on one side of the housing; The power supply device is connected to an external power source through the charging port for charging; the power supply device is respectively connected to the laser emission module, the multi-channel photoelectric detection module, the signal processing module and the result display module through the power switch for power supply.

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