A portable electric conversion device and electric conversion method based on an inductance boosting circuit

Abstract

The application discloses a portable electric conversion device and electric conversion method based on an inductance boosting circuit, which comprises an inductance boosting regulating circuit, an input end of which is connected with an output end of a storage battery, energy is stored through inductance, and after being regulated through a controller signal received by a feedback circuit through a field effect tube, output is generated; a filter circuit, which is connected with an output end of the inductance boosting regulating circuit; a polarity switching circuit, which is connected with an output end of the filter circuit and is output to an electric conversion output end; a pulse control circuit, which is parallel to the polarity switching circuit and is connected to the electric conversion output end; and a controller, which returns an output pulse signal collected by a sampling circuit to the controller, receives a user operation instruction, and outputs a regulating signal to the feedback circuit; and also sends a control signal to the polarity switching circuit and the pulse control circuit. The application improves portability, can guarantee effective regulation of output voltage, guarantee stability of output voltage, and improve electric conversion efficiency.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of electrotransformation equipment, and particularly relates to a portable electrotransformation equipment based on an inductance boosting circuit and an electrotransformation method. BACKGROUND

[0002] Transfection is a method of non-viral gene delivery into recipient cells, and the main purpose is to change the host genome to express or block the expression of proteins related to genes. By using this technology, the protein expression function of the target cell can be temporarily or permanently modified to give it unprecedented new functions.

[0003] Electrotransfection (electrotransformation), also known as cell electroporation, is an important method for introducing exogenous macromolecular substances such as DNA, RNA, siRNA, proteins, etc. and some small molecules into the cell membrane for expression in a manner similar to electrophoresis by using a strong instantaneous electric field or instantaneous current to temporarily increase the permeability of the cell membrane. By using electrotransfection, transfection of almost all eukaryotic cells can be completed, and it is suitable for research on transient or stable expression of proteins.

[0004] The electrotransformation instrument is usually a desktop device, which is powered by alternating current, and the output end is divided into positive and negative poles, each of which is a 4mm diameter banana jack socket. In actual application, the positive and negative electrodes are respectively connected and extended to the surface of the animal tissue organ containing the cell suspension or removed. When the electric pulse signal generated by the output end is transmitted to the electrode, due to the strong electric field or strong current, the cell membrane of the cells around the electrode will have strong permeability in a short time, thereby allowing exogenous macromolecules such as plasmids, RNA, proteins, drugs, etc. in the liquid environment around the cell membrane to enter the cell interior.

[0005] The prior art desktop electroporator adopts Cockcroft-Walton high-voltage multiplier, and is connected with alternating current power supply as a power source, occupies a large space, and needs a very high voltage, up to several hundred to several thousand volts, to complete the high-efficiency electroporation. In addition, since the capacitors in the Cockcroft-Walton high-voltage multiplier are in series discharge, the output high-order voltage multiplier has poor load capacity, and a small output power may cause a large drop in output voltage; and after each discharge, the number of capacitors that need to be charged increases with the number of voltage multiplier stages, resulting in that the output electric quantity cannot be supplemented in time. Therefore, when the impedance of the cell is small, or the distance between the electrodes used is very small, or the required electric pulse interval time is small, the actual output voltage cannot reach the set voltage, resulting in low electroporation efficiency. Moreover, it cannot directly act on the living body, and cannot be worn and implanted for in-situ electroporation. The prior art desktop electroporator device is relatively bulky, and the operation requires high professional degree, so that the activities of the subjects need to be limited during animal experiments or clinical experiments, and professional experimenters are needed to operate, increasing the difficulty and popularity of experiments. In particular, when RNA, siRNA and other fragile small molecules are used, or when cells such as nerve cells and stem cells are used, a super-clean environment needs to be provided, and a super-clean table is used. However, the currently marketed desktop electroporator is too large in size to be installed in the super-clean table equipped in a general laboratory.

[0006] It has been proved by current researches that in-situ electroporation is a better gene therapy method, which directly delivers DNA and plasmid DNA to the body tissue by electric assistance to achieve the purpose of gene therapy. A large number of gene delivery experiments have proved the feasibility of using electric pulses as a nucleic acid delivery system. Since the traditional electroporator is only suitable for in-vitro electroporation of cells and tissues, it is too large in size, needs to be connected with a plug, and cannot output multiple pulses at the same time, so that researchers need to make electrodes to meet different electric pulse requirements and detection requirements when using it for in-vivo electroporation. Moreover, the traditional electroporator is very unstable when the output voltage is small, and there is a risk in in-situ transfection. Therefore, there is no suitable instrument for in-situ electroporation at present, and researchers have been suffering from the difficulty of directly performing fast and effective in-situ electroporation. SUMMARY

[0007] To solve the above problems, the present application provides a portable electroporation device and method based on an inductive boost circuit, which improves portability, ensures effective adjustment of output voltage, ensures stability of output voltage, improves electroporation efficiency, and can directly perform in-situ electroporation such as wearing and implantation.

[0008] In order to achieve the above object, the technical scheme adopted by the present application is: a portable electro-transformation device based on an inductive boost circuit, comprising a battery and an electro-transformation regulator arranged in a shell, and an electro-transformation electrode connected to an output end of the electro-transformation regulator.

[0009] The electro-transformation regulator comprises:

[0010] An inductive boost regulating circuit, an input end of which is connected to an output end of the battery, stores energy through an inductor and outputs after being regulated by a controller signal received from a feedback circuit through a field effect tube;

[0011] A filter circuit, which is connected to an output end of the inductive boost regulating circuit;

[0012] A polarity switching circuit, which is connected to an output end of the filter circuit and outputs to an electro-transformation output end;

[0013] A pulse control circuit, which is parallel to the polarity switching circuit and connected to the electro-transformation output end;

[0014] And a controller, which returns an output pulse signal collected by a sampling circuit to the controller, receives a user operation instruction, outputs a regulating signal to the feedback circuit, and sends a control signal to the polarity switching circuit and the pulse control circuit.

[0015] Further, the inductive boost regulating circuit comprises an input capacitor, an inductor, a field effect tube, a diode and an output capacitor, the input end of the inductive boost regulating circuit is provided with the input capacitor, the inductor, the field effect tube and the diode are connected in Y shape, the inductor is connected to the input end of the inductive boost regulating circuit, the diode is connected to the output end of the inductive boost regulating circuit, and the output capacitor is arranged at the output end of the inductive boost regulating circuit.

[0016] Further, the feedback circuit regulates the field effect tube through a PWM signal generating circuit.

[0017] Further, a driving amplifier is arranged after the PWM signal generating circuit, and the PWM wave is generated by the PWM signal generating circuit and the driving amplifier, which is a periodic wave to control the field effect tube to be periodically turned on and off.

[0018] Further, the controller sends the control signal to the polarity switching circuit and the pulse control circuit through the driving amplifier.

[0019] Further, a man-machine interaction interface device is arranged on the controller to obtain the user operation instruction and show it to the user.

[0020] On the other hand, the electro-transformation method of the portable electro-transformation device based on the inductive boost circuit comprises the following steps:

[0021] The controller uses a feedback circuit to control the inductor-driven boost regulation circuit to adjust the battery output power to the required voltage level pulse.

[0022] The output pulse after being regulated by the inductor boost regulator circuit is filtered by a filter circuit.

[0023] The filtered output pulse switches the polarity of the output voltage through a polarity switching circuit, thereby adjusting the polarity of the output pulse.

[0024] The pulse parameters are adjusted by a pulse control circuit.

[0025] The final output pulse is a pulse sequence consisting of multiple DC pulses, which is applied to the surface of cells, tissues, or organs to complete electroporation.

[0026] Meanwhile, the sampling circuit collects the output voltage pulse signal in real time and feeds it back to the human-machine interface to display the actual output waveform in real time. If the actual output voltage waveform does not meet the set requirements, the controller adjusts the output voltage in real time by switching the feedback circuit and the filtering circuit to meet the requirements of high-efficiency electro-conversion.

[0027] Furthermore, the controller uses a feedback circuit to control the inductor-driven boost regulation circuit to adjust the battery output power to the required voltage level, including the following steps:

[0028] When the field-effect transistor of the inductor boost regulation circuit is turned on, the battery charges the inductor, and the current through the inductor increases linearly at a set rate. As the inductor current increases, energy is stored in the inductor.

[0029] Disconnect the MOSFET in the inductor boost regulator circuit, and the current flowing through the inductor charges the output capacitor. During this period, the inductor discharges, its current gradually decreases, and the output voltage gradually increases until the end of the cycle. At this time, the output voltage is Vout1.

[0030] When the MOSFET of the boost regulator circuit is turned on again, the battery charges the inductor again; and due to the unidirectional conduction characteristic of the diode, the charge accumulated in the output capacitor will not be lost, so the output voltage remains at Vout1.

[0031] Disconnect the MOSFET of the boost regulator circuit again, and the current flowing through the inductor will charge the output capacitor again. The output voltage will continue to rise from Vout1 to the output voltage value set by the controller.

[0032] Further, the feedback circuit regulates the field effect transistor through the PWM signal generation circuit; a drive amplifier is arranged after the PWM signal generation circuit, and the PWM wave generated by the PWM signal generation circuit and the drive amplifier is a periodic wave, so as to control the field effect transistor to be periodically turned on and turned off, and the output voltage continues to rise to the output voltage value set by the controller.

[0033] The beneficial effects of the technical scheme are as follows:

[0034] The output pulse regulated by the inductive boost regulating circuit is filtered through the filtering circuit; the output pulse after filtering is switched by the polarity switching circuit to switch the positive and negative poles of the output voltage, so as to regulate the polarity of the output pulse; the pulse parameter is regulated by the pulse control circuit; finally, the output pulse is a pulse sequence composed of multiple direct current pulses, which is applied to the surface of cells, tissues or organs for completing the electroporation; at the same time, the sampling circuit collects the output voltage pulse signal in real time and feeds back to the human-computer interaction interface to display the actual output waveform in real time; if the actual output voltage waveform does not meet the set requirement, the controller adjusts the output voltage in real time by switching the feedback circuit and the filtering circuit, so as to meet the demand of high-efficiency electroporation; thus, the effective regulation of the output voltage can be ensured, the stability of the output voltage can be ensured, and the electroporation efficiency can be improved, and the device can be directly worn and implanted.

[0035] The inductive boost regulating circuit is used for high-speed energy transmission, so that charging can be performed in a very short period, thereby generating a pulse with ultra-short time resolution. The pulse width resolution generated by the present application can reach 100 ns, so that the vitality of cells can be better maintained during the electroporation process.

[0036] The charging battery power supply scheme is adopted to greatly reduce the size and weight of the electroporation instrument, and the miniaturization and portability of the electroporation instrument are realized through the boost conversion circuit, the capacitor energy storage, the pulse instantaneous discharge and other technologies. The power management, the boost circuit and the pulse instantaneous discharge circuit are integrated on the chip through the high-voltage chip design, and the portable or implantable electroporation device is realized by using the battery power supply. This will greatly expand the application of the electrogene therapy technology and the electroporation drug delivery technology in the clinic, make up for the deficiency of the existing gene therapy technology, and greatly improve the accessibility of the gene therapy and increase the indications of the gene therapy.

[0037] The inductor device has a smaller size, so that a more miniaturized high-voltage pulse output scheme can be realized, and is suitable for an environment with a higher integration requirement and an output voltage pulse amplitude of 100 V or less. BRIEF DESCRIPTION OF DRAWINGS

[0038] Figure 1 It is a structure schematic diagram of a portable electroporation device based on an inductive boost circuit.

[0039] Figure 2 This is a schematic flowchart of a portable EDM method based on an inductor boost circuit according to an embodiment of the present invention.

[0040] Figure 3 This is a schematic diagram of the adjustment of the inductor boost regulator circuit in an embodiment of the present invention;

[0041] Figure 4 This is a schematic diagram of the transfection efficiency in an embodiment of the present invention.

[0042] In this diagram, 11 is the input capacitor, 12 is the inductor, 13 is the field-effect transistor, 14 is the diode, and 15 is the output capacitor. Detailed Implementation

[0043] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described below with reference to the accompanying drawings.

[0044] In this embodiment, see Figure 1 As shown, the present invention proposes a portable power transfer device based on an inductor boost circuit, including a battery and a power transfer regulator disposed in the housing, wherein the output terminal of the power transfer regulator is connected to a power transfer electrode;

[0045] The electric regulator includes:

[0046] The inductor boost regulator circuit has its input terminal connected to the output terminal of the battery. It stores energy through inductor 12 and receives controller signals through field-effect transistor 13 before outputting the regulator.

[0047] The filter circuit is connected to the output terminal of the inductor boost regulator circuit;

[0048] The polarity switching circuit is connected to the output of the filter circuit and outputs to the output of the electric motor.

[0049] The pulse control circuit, in parallel with the polarity switching circuit, is connected to the output terminal of the electric motor.

[0050] The sampling circuit collects the output pulse signal and returns it to the controller. The controller receives the user's operation command and outputs the adjustment signal to the feedback circuit. It also sends control signals to the polarity switching circuit and the pulse control circuit.

[0051] Based on the same inventive concept, such as Figure 2 As shown, the present invention also provides a method for electro-electric transfer based on the above-mentioned portable electro-electric transfer device based on an inductor boost circuit, comprising the following steps:

[0052] The controller uses a feedback circuit to control the inductor-driven boost regulation circuit to adjust the battery output power to the required voltage level pulse.

[0053] The output pulse of the inductive boost regulating circuit is filtered by a filter circuit;

[0054] The filtered output pulse is switched by a polarity switching circuit to switch the positive and negative poles of the output voltage, thereby regulating the polarity of the output pulse;

[0055] The pulse parameters, including the number of pulses, interval time, and high-level duty cycle, are regulated by a pulse control circuit;

[0056] The final output pulse is a pulse sequence composed of multiple direct current pulses, which is applied to the surface of cells, tissues, or organs for completing electroporation;

[0057] At the same time, the sampling circuit collects the output voltage pulse signal in real time and feeds it back to the human-computer interaction interface to display the actual output waveform in real time. If the actual output voltage waveform does not meet the set requirements, the controller adjusts the output voltage in real time by switching the feedback circuit and the filter circuit to meet the demand of efficient electroporation.

[0058] As an optimization scheme of the above embodiment, the inductive boost regulating circuit comprises an input capacitor 11, an inductor 12, a field effect transistor 13, a diode 14, and an output capacitor 15. The input end of the inductive boost regulating circuit is provided with the input capacitor 11. The inductor 12, the field effect transistor 13, and the diode 14 form a Y-shaped connection. The inductor 12 is connected to the input end of the inductive boost regulating circuit. The diode 14 is connected to the output end of the inductive boost regulating circuit. The output capacitor 15 is arranged at the output end of the inductive boost regulating circuit.

[0059] The controller controls the inductive boost regulating circuit to regulate the output power of the battery to the required voltage level for electroporation through the feedback circuit, including the steps of:

[0060] The field effect transistor 13 of the inductive boost regulating circuit is turned on. The battery charges the inductor 12, and the current flowing through the inductor 12 increases linearly at a set rate. As the current of the inductor 12 increases, energy is stored in the inductor 12;

[0061] The field effect transistor 13 of the inductive boost regulating circuit is turned off. The current flowing through the inductor 12 charges the output capacitor 15. During this period, the inductor 12 discharges, and its current gradually decreases, while the output voltage gradually increases until the end of the period, at which time the output voltage is Vout1;

[0062] The field effect transistor 13 of the inductive boost regulating circuit is turned on again. The battery charges the inductor 12 again. Due to the unidirectional conduction characteristic of the diode 14, the charge accumulated in the output capacitor 15 does not flow away, so that the output voltage remains Vout1;

[0063] The field effect transistor 13 of the boost regulating circuit is disconnected again, the current flowing through the inductor 12 charges the output capacitor 15 again, and the output voltage continues to rise to the output voltage value set by the controller on the basis of Vout1.

[0064] According to the above principle, the output voltage will continue to rise theoretically until the output capacitor 15 is broken down. However, in practice, the reverse isolation resistance of the diode 14 is not infinite, and the final output voltage VOUT of the circuit is output to a finite load impedance (including the finite impedance of the filter and polarity switching circuit, and the finite impedance of other external loads), and the capacitor will discharge through the diode 14 and the load impedance, so the output voltage cannot be infinitely high. If the output voltage needs to be accurately controlled, a feedback circuit needs to be introduced. In the present application, the feedback circuit can be controlled by the output port of the controller; and since the controller can be controlled by the program through the human-computer interaction interface, the operator can conveniently control the output voltage of the electrotransformation device through the human-computer interaction interface.

[0065] The continuous charging and discharging process of the output capacitor 15 causes the output voltage to fluctuate continuously, so the present application also introduces a filter module to enable the output voltage to be more stably controlled. The polarity switching circuit can switch the positive and negative poles of the output voltage, so that the polarity of the output pulse of the electrotransformation device can be controlled according to the setting of the operator. In addition, the number, interval time, and high-level duty cycle of the pulse can be adjusted through the pulse control circuit. The drive amplifier is used to amplify the signal generated by the controller, so as to drive the polarity switching circuit and the pulse control circuit.

[0066] The final output voltage VOUT is a pulse sequence composed of multiple highly personalized and parameter-adjustable direct current pulses, which can be applied to the surface of cells, tissues, or organs for completing electrotransformation. The sampling circuit collects the output voltage pulse signal in real time, and converts it into a digital signal by an analog-to-digital converter (which can be integrated inside the controller or can be a separate device), and feeds it back to the human-computer interaction interface to display the actual output waveform in real time. If the actual output voltage waveform does not meet the set requirements, the controller adjusts the output voltage in real time by switching the feedback circuit and the filter circuit to meet the demand of efficient electrotransformation.

[0067] Preferably, the feedback circuit regulates the field effect transistor 13 through a PWM signal generating circuit; a drive amplifier is arranged after the PWM signal generating circuit, and the PWM signal generating circuit and the drive amplifier cooperate to generate a periodic wave to control the field effect transistor 13 to be turned on and off periodically, so that the output voltage continues to rise to the output voltage value set by the controller.

[0068] As an optimization of the above-mentioned embodiment, the controller sends control signals to the polarity switching circuit and the pulse control circuit through the driving amplifier.

[0069] As an optimization of the above-mentioned embodiment, a human-computer interaction interface device is arranged on the controller, which is used to obtain user operation instructions and show the user.

[0070] Due to the real-time performance of the inductive boost regulating circuit, the output voltage can be adjusted in real time by the controller, so as to adjust the output pulse waveform, which can be any waveform, such as square wave, sine wave, triangular wave, exponential decay wave, dynamic decay wave, etc., as well as a combination of multiple different waveforms and amplitudes. According to the membrane potential of different cells, symmetric or asymmetric perforation pulses can be used to effectively improve the permeability of the cell membrane. Figure 3 (a)-(e) show, Figure 3 The abscissa of (a)-(e) is time, and the ordinate is amplitude. The function realized by the present application is to generate a sequence of pulses with different amplitudes and change laws for the application of cell electroporation transfection. The electric conversion device involved in the present application usually outputs two groups of pulses: perforation pulses and electric conversion pulses. The pulse sequence with high amplitude but short duration is called perforation pulse, which can generate a strong electric field on the surface of the cell membrane, thereby enhancing the permeability of the cell membrane; the pulse sequence with low amplitude but long duration is called electric conversion pulse, which uses dielectrophoresis force to send exogenous macromolecules such as plasmid, RNA, protein, and drug in the liquid environment around the cell membrane into the cell. The duration of the perforation pulse should not be too long, otherwise it will cause irreversible damage to the cell membrane; the membrane potential of different cells is different, so the amplitude, time, and symmetry of the optimal perforation pulse and electric conversion pulse for different cells are different.

[0071] Unlike the Cockcroft-Walton high-voltage multiplier, the present application does not perform voltage boosting through a series voltage multiplying circuit, but performs high-speed energy transfer through an inductive boost regulating circuit, so that charging can be performed in a very short period of time, thereby generating pulses with ultra-short time resolution.

[0072] In the conventional electric conversion device using Cockcroft-Walton high-voltage multiplier, the resolution of pulse width is usually above 10us, and high voltage can easily cause irreversible damage to the cell membrane. Due to the real-time performance of the inductive boost regulating circuit, the resolution of the pulse width generated by the present application can reach the level of 100ns, so that the viability of the cells can be better maintained during the electric conversion process.

[0073] The size of the inductor 12 device is smaller, so that a more miniaturized high-voltage pulse output scheme can be realized, which is suitable for an environment with a higher requirement for integration, with the output voltage pulse amplitude being within 100V.

[0074] The application greatly reduces the volume of the electrotransformation instrument through high integration, and uses battery power supply, greatly improves the flexibility, and is very suitable for in situ electrotransformation experiment. In the research of the effect of in situ electroporation in different tissues, different tissues need different voltage pulses, and the voltage is different, and when in situ electrotransfection is carried out, in order not to cause too much damage to the tissue, the voltage used is mostly low voltage long pulse. The application accurately controls the output voltage, output pulse under the condition of ensuring low voltage output, and can be flexibly adjusted according to different tissues to meet different experimental needs.

[0075] The effect of the device is proved by specific experiments. In the experiment of delivering SiRNA into SD rat epithelial cells, the rat epithelial cells used are relatively fragile, high in voltage sensitivity and poor in high voltage tolerance. SiRNA delivery requires a stable pulse of about 100V to achieve the effect of electroporation. Ordinary square wave pulse and exponential pulse will repeatedly produce transient high voltage in a short period, which is extremely harmful to cells and can easily lead to irreversible electroporation. The pulse generated by the device can gradually increase and decrease the current intensity on the surface of the cells, effectively balancing the survival rate of the cells and the electroporation effect. For example Figure 4 In the siRNA delivery experiment, Cy5 red dye is used to confirm the transfection efficiency. When using the commercially available Cockcroft-Walton electrotransformation instrument for delivery, using 150V square wave pulse for experiment can greatly reduce the survival rate of cells and seriously affect the transfection efficiency, such as Figure 4 The left part of Fig. 2; while using the application can maintain good transfection effect, such as Figure 4 The right part of Fig. 2. Using sensing software to count the number of successfully transfected cells in a 500um*500um random field (randomly select 5 fields per well and take the average), the number of successfully transfected cells (average = 90) by the electrotransformation instrument used in the application is significantly higher than that (average = 14) by the Cockcroft-Walton electrotransformation instrument.

[0076] The basic principles and main features of the application and the advantages of the application are shown and described above. Those skilled in the art should understand that the application is not limited by the above examples, and the above examples and descriptions in the specification are only to illustrate the principles of the application, and various changes and improvements can be made without departing from the spirit and scope of the application, and these changes and improvements all fall within the scope of the claimed application. The scope of protection of the application is defined by the appended claims and their equivalents.

Claims

1. A portable electric conversion device based on an inductive boost circuit, characterized by comprising: The portable electroporation device based on the inductive boost circuit comprises: an inductive boost regulating circuit, an input end of which is connected to an output end of a battery, and the inductive boost regulating circuit stores energy through an inductor and outputs after being regulated by a feedback circuit receiving a controller signal through a field effect transistor; a filter circuit, which is connected to an output end of the inductive boost regulating circuit; a polarity switching circuit, which is connected to an output end of the filter circuit and outputs to an electroporation output end; a pulse control circuit, which is parallel to the polarity switching circuit and is connected to the electroporation output end; and a controller, which returns an output pulse signal collected by a sampling circuit to the controller, receives a user operation instruction, outputs a regulating signal to the feedback circuit, and sends a control signal to the polarity switching circuit and the pulse control circuit. The positive and negative poles of the output voltage are switched through the polarity switching circuit, so as to regulate the pulse polarity; the pulse parameters are regulated through the pulse control circuit; and finally, the output pulse is a pulse sequence composed of multiple direct current pulses, which is applied to the surface of cells, tissues or organs to complete electroporation. If the actual output voltage waveform does not meet the set requirements, the controller adjusts the output voltage in real time by switching the feedback circuit and the filter circuit. The inductive boost regulating circuit comprises an input capacitor, an inductor, a field effect transistor, a diode and an output capacitor, the input end of the inductive boost regulating circuit is provided with the input capacitor, the inductor, the field effect transistor and the diode are connected in a Y shape, the inductor is connected to the input end of the inductive boost regulating circuit, the diode is connected to the output end of the inductive boost regulating circuit, and the output capacitor is arranged at the output end of the inductive boost regulating circuit. The feedback circuit regulates the field effect transistor through a PWM signal generating circuit. A driving amplifier is arranged after the PWM signal generating circuit, and the PWM signal generating circuit and the driving amplifier cooperatively generate a PWM wave which is a periodic wave to control the field effect transistor to be periodically turned on and off. The controller sends a control signal to the polarity switching circuit and the pulse control circuit through the driving amplifier. A human-computer interaction interface device is arranged on the controller to obtain a user operation instruction and show the user. The portable electroporation device based on the inductive boost circuit comprises: an inductive boost regulating circuit, an input end of which is connected to an output end of a battery, and the inductive boost regulating circuit stores energy through an inductor and outputs after being regulated by a feedback circuit receiving a controller signal through a field effect transistor; a filter circuit, which is connected to an output end of the inductive boost regulating circuit; a polarity switching circuit, which is connected to an output end of the filter circuit and outputs to an electroporation output end; a pulse control circuit, which is parallel to the polarity switching circuit and is connected to the electroporation output end; and a controller, which returns an output pulse signal collected by a sampling circuit to the controller, receives a user operation instruction, outputs a regulating signal to the feedback circuit, and sends a control signal to the polarity switching circuit and the pulse control circuit.

2. A portable electrically powered device based on an inductive boost circuit according to claim 1, characterized in that, The electroporation method based on the portable electroporation device based on the inductive boost circuit comprises the following steps:

3. A portable electrically powered device based on an inductive boost circuit according to claim 1 or 2, characterized in that The controller controls the inductive boost regulating circuit to regulate the output power of the battery to a voltage level pulse required by electroporation through the feedback circuit.

4. A portable electrically powered device based on an inductive boost circuit according to claim 3, characterized in that, ​ 5. A portable electric conversion device based on an inductance boosting circuit according to claim 1, wherein ​ 6. A portable electric conversion device based on an inductance boosting circuit according to claim 1, wherein ​ 7. An electrical rotation method of a portable electrical rotation device based on an inductance boosting circuit, characterized by, ​ ​ ​ The output pulse of the inductive boost regulating circuit is filtered by a filter circuit; The filtered output pulse is switched by a polarity switching circuit to switch the positive and negative poles of the output voltage, thereby adjusting the polarity of the output pulse; The pulse parameters are adjusted by a pulse control circuit; The final output pulse is a pulse sequence composed of multiple direct current pulses, which is applied to the surface of cells, tissues, or organs for completing electroporation; If the actual output voltage waveform does not meet the set requirements, the controller adjusts the output voltage in real time by switching the feedback circuit and the filter circuit; At the same time, the sampling circuit collects the output voltage pulse signal in real time and feeds it back to the human-computer interaction interface to display the actual output waveform in real time; if the actual output voltage waveform does not meet the set requirements, the controller adjusts the output voltage in real time by switching the feedback circuit and the filter circuit to meet the demand of efficient electroporation.

8. The method of claim 7, wherein the portable electric conversion device is a portable electric conversion device based on an inductance boosting circuit. The inductive boost regulating circuit comprises an input capacitor, an inductor, a field effect transistor, a diode, and an output capacitor, the input end of the inductive boost regulating circuit is provided with the input capacitor, the inductor, the field effect transistor, and the diode are connected in a Y shape, the inductor is connected to the input end of the inductive boost regulating circuit, the diode is connected to the output end of the inductive boost regulating circuit, and the output capacitor is arranged at the output end of the inductive boost regulating circuit; The controller controls the inductive boost regulating circuit to adjust the output power of the battery to the voltage level required for electroporation through the feedback circuit, including the steps of: The field effect transistor of the inductive boost regulating circuit is turned on, the battery charges the inductor, and the current through the inductor increases linearly at a set rate, and as the inductor current increases, energy is stored in the inductor; The field effect transistor of the inductive boost regulating circuit is turned off, and the current flowing through the inductor charges the output capacitor; during this period, the inductor discharges, and its current gradually decreases, while the output voltage gradually increases until the end of the period, at which time the output voltage is Vout1; The field effect transistor of the inductive boost regulating circuit is turned on again, and the battery charges the inductor again; due to the unidirectional conduction characteristic of the diode, the charge accumulated in the output capacitor does not flow away, so that the output voltage remains Vout1; The field effect transistor of the inductive boost regulating circuit is turned off again, and the current flowing through the inductor charges the output capacitor again, and the output voltage continues to rise to the output voltage value set by the controller based on Vout1.

9. The method of claim 8, wherein the portable electric conversion device is a portable electric conversion device based on an inductance boosting circuit. The feedback circuit controls the field effect transistor through a PWM signal generating circuit; a driving amplifier is arranged after the PWM signal generating circuit, and the PWM signal generating circuit and the driving amplifier cooperate to generate a periodic PWM wave to control the field effect transistor to be turned on and off periodically, and the output voltage continues to rise to the output voltage value set by the controller.

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