A Highly Effective Hydrating Toner and Its Preparation Method

Abstract

This invention discloses a highly effective moisturizing toner and its preparation method, relating to the field of cosmetic technology. The highly effective moisturizing toner prepared by this invention includes a highly effective moisturizing factor and a compound preservative. The highly effective moisturizing factor is obtained by mixing hesperidin and collagen, placing them in the extracellular fluid after Ganoderma lucidum fermentation, and then performing micellar solubilization, thereby enhancing the antioxidant properties of the toner. A compound preservative is formed by combining polyaminopropyl biguanide, cetylpyridinium chloride, and capryloyl hydroxamic acid, which not only synergistically inhibits bacteria and provides preservation but also possesses mildness, low irritation, and strong antibacterial effect.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a highly effective moisturizing toner and its preparation method. Background Technology

[0002] With the improvement of living standards, people are paying more and more attention to skin care. However, various skin problems caused by internal and external factors are still occurring. First of all, the skin is composed of three layers: the epidermis, the dermis, and the subcutaneous fat. The dermis contains collagen, elastin, and other fibers that form the framework supporting the skin. These elements make the skin appear smooth and youthful. However, these elements are also damaged by UVA, UVB, ozone, or other oxidizing factors. Moreover, modern people face high pressure, work overtime, stay up late, spend long hours in front of computers, smoke, lack sleep, stay in air-conditioned rooms for extended periods, drink alcohol, and overuse their eyes. These have become the norm. Coupled with air pollution, a large number of cellular and molecular toxins caused by internal and external factors accumulate in each layer of the skin every day. This can lead to skin cell suffocation and affect the skin's own ability to clear toxins, resulting in problems such as dull skin tone, lack of radiance, and fatigue, which increases the burden on the skin and accelerates skin aging.

[0003] Therefore, cosmetics targeting various skin problems have emerged. Toner, also known as astringent or lotion, is used to cleanse the skin again to restore its pH balance, condition the stratum corneum, improve skin absorption, and prepare the skin for the use of skincare products. It can quickly replenish moisture. Therefore, this invention has developed a highly effective moisturizing toner that can inhibit bacteria and inflammation, and has antioxidant and anti-aging properties. Summary of the Invention

[0004] The purpose of this invention is to provide a highly effective moisturizing toner and its preparation method, so as to solve the problems mentioned in the background art.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a highly effective moisturizing toner, wherein the highly effective moisturizing toner comprises purified water, a moisturizer, a highly effective moisturizing factor, and a compound preservative.

[0006] Preferably, the highly effective moisturizing factor is prepared by mixing hesperidin and collagen, placing them in the extracellular fluid after Ganoderma lucidum fermentation, and then performing micellar solubilization.

[0007] Preferably, the composite preservative includes polyaminopropyl biguanide, cetylpyridinium chloride, and octanoyl hydroxamic acid.

[0008] Preferably, the moisturizer includes glycerin, 1,2-hexanediol, and hyaluronic acid.

[0009] Preferably, the preparation method of the highly effective moisturizing toner includes the following specific steps:

[0010] (1) Sterilize a 3-4% Ganoderma lucidum powder solution at 121℃ for 30-40 min, inoculate it into the basal culture medium, sterilize it again, and take a 0.5 cm sample from the culture plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7-10 days to obtain a primary seed culture solution. The primary seed culture solution was then inoculated at 10% into the basal medium, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7-10 days to obtain a secondary seed culture. This secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate... 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7-10 days to obtain the fermentation broth, which was then filtered through 60-mesh and 200-mesh filter cloths to obtain the Ganoderma lucidum fermentation extracellular fluid.

[0011] (2) Mix the mixture with the Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:50~1:100, heat to 60~80℃, keep warm for 10~30 min, add 10~30 times the mass of the mixture of hyaluronic acid solution with a mass fraction of 0.5~0.8%, stir evenly at 600~1000 rpm, and pass through a 100~200 mesh sieve to obtain a high-efficiency hydrating factor;

[0012] (3) Place pure water in an emulsification pot, heat it to 60~80℃, add high-efficiency moisturizing factor and moisturizer, stir at 20~50rpm for 5~10min, add compound preservative, and continue stirring for 30~50min to obtain high-efficiency moisturizing toner.

[0013] Preferably, in step (1) above: the basal culture medium includes 30 g / L anhydrous glucose, 3 g / L FM801 active dry yeast powder, 2 g / L potassium dihydrogen phosphate, and 2 g / L magnesium sulfate heptahydrate, and the sterilization conditions are sterilization at 121℃ for 30 min.

[0014] Preferably, in step (2) above, the preparation method of the mixture is as follows: hesperidin and anhydrous ethanol are mixed at a mass ratio of 1:8 to 1:16, ultrasonically dispersed at 50 to 80 kHz for 8 to 10 minutes, then Tween 20 at 2 to 4 times the mass of hesperidin and collagen at 0.4 to 0.6 times the mass of hesperidin are added, ultrasonically dispersed for 5 to 8 minutes, and passed through a 100 to 200 mesh sieve to obtain the mixture.

[0015] Preferably, in step (3) above, the mass ratio of polyaminopropyl biguanide, cetyl chloride and octanoyl hydroxamic acid in the composite preservative is 15:2:8~30:6:9.

[0016] Preferably, in step (3) above, the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the moisturizer is 2:2:0.3~5:4:0.5.

[0017] Preferably, in step (3) above, the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 70:7:3:0.5~80:10:5:1.

[0018] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0019] The highly effective moisturizing toner prepared by this invention includes highly effective moisturizing factors and compound preservatives;

[0020] The highly effective moisturizing factor is prepared by mixing hesperidin and collagen, then placing them in the extracellular fluid of fermented Ganoderma lucidum for micellar solubilization. Hesperidin is a flavonoid compound with antioxidant and anti-inflammatory activities, but it has poor water solubility. By attaching collagen to the surface of hesperidin, the solubility of hesperidin is enhanced. Then, it is placed in the extracellular fluid of fermented Ganoderma lucidum with antioxidant properties for solubilization, which further improves water solubility and enhances the antioxidant properties of the toner.

[0021] Polyaminopropyl biguanide contains positively charged active guanidine groups, which can be adsorbed by negatively charged bacteria and viruses, inhibiting their division and forming a polymer film that blocks the respiratory channels of microorganisms, causing them to suffocate and die rapidly. However, its inhibitory effect on mold is relatively poor. Cetylpyridinium chloride can disrupt cell integrity, causing intracellular contents to leak out and destroying enzymes bound to the cell membrane, thereby disrupting metabolism. Capryloyl hydroxamic acid can be used as a chelating agent in cosmetics. It is a non-allergenic ingredient, non-carcinogenic and non-teratogenic, biodegradable, and can efficiently chelate iron ions, thus limiting the growth of mold in an iron-deficient environment and achieving antibacterial and preservative effects. Combining polyaminopropyl biguanide, cetylpyridinium chloride, and capryloyl hydroxamic acid forms a composite preservative that not only synergistically inhibits bacteria and preserves but also has mildness, low irritation, and strong antibacterial effect. Detailed Implementation

[0022] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0023] To more clearly illustrate the method provided by the present invention, the following embodiments are provided for detailed explanation. The testing methods for various indicators of the highly effective moisturizing toners prepared in the embodiments and comparative examples are as follows:

[0024] Antioxidant properties: The high-efficiency moisturizing toners prepared in the examples and comparative examples were subjected to a cleansing rate test. The test method is as follows:

[0025] (1) Prepare DPPH solution;

[0026] (2) Add 10 mL of anhydrous ethanol to 10 mL of 0.2 mmol / L DPPH solution, stir well, let stand for 10 min, and measure the absorbance A. C Add 10 mL of the pumpkin extract firming and anti-wrinkle molecular mask prepared in Example 1 to 10 mL of 0.2 mmol / L DPPH solution, stir well and let stand for 10 min, and measure the absorbance as A1. Mix 10 mL of the pumpkin extract firming and anti-wrinkle molecular mask prepared in Example 1 with 10 mL of anhydrous ethanol and stir well, and measure the absorbance as A2.

[0027]

[0028] Antibacterial properties: After the highly effective moisturizing toners prepared in the examples and comparative examples were left to stand for one week, the upper liquid was taken for antibacterial properties testing. Example 1

[0029] (1) Sterilize a 3% Ganoderma lucidum powder solution at 121℃ for 30 min, inoculate it into the basal culture medium, sterilize it again, and take a 0.5 cm sample from the culture plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7 days to obtain a primary seed culture. The primary seed culture was then inoculated into the basal medium at a concentration of 10%, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7 days to obtain a secondary seed culture. The secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7 days to obtain the fermentation broth, which was then filtered through 60-mesh and 200-mesh filter cloths to obtain the Ganoderma lucidum fermentation extracellular fluid. The basic culture medium included 30 g / L anhydrous glucose, 3 g / L FM801 active dry yeast powder, 2 g / L potassium dihydrogen phosphate, and 2 g / L magnesium sulfate heptahydrate. The sterilization conditions were 121℃ for 30 min.

[0030] (2) Mix hesperidin and anhydrous ethanol at a mass ratio of 1:8, sonicate at 50~80kHz for 8min, then add Tween 20 at 2 times the mass of hesperidin and collagen at 0.4 times the mass of hesperidin, continue sonicating for 5min, and pass through a 100-mesh sieve to obtain a mixture; mix the mixture with Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:50, heat to 60℃, keep warm for 10min, then add hyaluronic acid solution with a mass fraction of 0.5% at 10 times the mass of the mixture, stir evenly at 600rpm, and pass through a 100-mesh sieve to obtain a high-efficiency moisturizing factor;

[0031] (3) Place pure water in an emulsification pot, heat it to 60°C, add high-efficiency moisturizing factor and humectant, stir at 20 rpm for 5 min, add compound preservative, and continue stirring for 30 min to obtain high-efficiency moisturizing toner; the mass ratio of polyaminopropyl biguanide, cetyl chloride and capryloyl hydroxamic acid in the compound preservative is 15:2:8; the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the humectant is 2:2:0.3; the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 70:7:3:0.5. Example 2

[0032] (1) Sterilize a 3.5% Ganoderma lucidum powder solution at 121℃ for 35 min, inoculate it into the basal culture medium, sterilize it again, and then take a 0.5 cm sample from the culture plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a primary seed culture. The primary seed culture was then inoculated into the basal medium at a concentration of 10%, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a secondary seed culture. The secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain the fermentation broth, which was then filtered through 60-mesh and 200-mesh filter cloths to obtain the Ganoderma lucidum fermentation extracellular fluid. The basic culture medium included 30 g / L anhydrous glucose, 3 g / L FM801 active dry yeast powder, 2 g / L potassium dihydrogen phosphate, and 2 g / L magnesium sulfate heptahydrate. The sterilization conditions were 121℃ for 30 min.

[0033] (2) Mix hesperidin and anhydrous ethanol at a mass ratio of 1:12, sonicate at 65 kHz for 9 min, then add 3 times the mass of hesperidin Tween 20 and 0.5 times the mass of hesperidin collagen, continue sonicating for 6 min, and pass through a 150 mesh sieve to obtain a mixture; mix the mixture with Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:75, heat to 70℃, keep warm for 20 min, then add 20 times the mass of the mixture of 0.65% hyaluronic acid solution, stir evenly at 80 rpm, and pass through a 150 mesh sieve to obtain a high-efficiency moisturizing factor;

[0034] (3) Place pure water in an emulsification pot, heat to 70°C, add high-efficiency moisturizing factor and humectant, stir at 35 rpm for 8 min, add compound preservative, and continue stirring for 40 min to obtain high-efficiency moisturizing toner; the mass ratio of polyaminopropyl biguanide, cetyl chloride and capryloyl hydroxamic acid in the compound preservative is 25:4:8.5; the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the humectant is 4:3:0.4; the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 75:8:4:0.8. Example 3

[0035] (1) Sterilize a 4% Ganoderma lucidum powder solution at 121℃ for 40 min, inoculate it into the basal culture medium, sterilize it again, and take a 0.5 cm sample from the culture plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 10 days to obtain a primary seed culture solution. The primary seed culture solution was then inoculated into the basal medium at a concentration of 10%, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 10 days to prepare a secondary seed culture. This secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate... 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 10 days to obtain the fermentation broth, which was then filtered through 60-mesh and 200-mesh filter cloths to obtain the Ganoderma lucidum fermentation extracellular fluid. The basic culture medium included 30 g / L anhydrous glucose, 3 g / L FM801 active dry yeast powder, 2 g / L potassium dihydrogen phosphate, and 2 g / L magnesium sulfate heptahydrate. The sterilization conditions were 121℃ for 30 min.

[0036] (2) Mix hesperidin and anhydrous ethanol at a mass ratio of 1:16, sonicate at 80 kHz for 10 min, then add 4 times the mass of hesperidin Tween 20 and 0.6 times the mass of hesperidin collagen, continue sonicating for 8 min, and pass through a 200-mesh sieve to obtain a mixture; mix the mixture with Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:100, heat to 80℃, keep warm for 30 min, then add 30 times the mass of the mixture of 0.8% hyaluronic acid solution, stir evenly at 1000 rpm, and pass through a 200-mesh sieve to obtain a high-efficiency moisturizing factor;

[0037] (3) Place pure water in an emulsification pot, heat it to 80°C, add high-efficiency moisturizing factor and humectant, stir at 50 rpm for 10 min, add compound preservative, and continue stirring for 50 min to obtain high-efficiency moisturizing toner; the mass ratio of polyaminopropyl biguanide, cetyl chloride and capryloyl hydroxamic acid in the compound preservative is 30:6:9; the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the humectant is 5:4:0.5; the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 80:10:5:1.

[0038] Comparative Example 1

[0039] (1) Sterilize a 3.5% Ganoderma lucidum powder solution at 121℃ for 35 min, inoculate it into the basal culture medium, sterilize it again, and then take a 0.5 cm sample from the culture plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a primary seed culture. The primary seed culture was then inoculated into the basal medium at a concentration of 10%, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a secondary seed culture. The secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain the fermentation broth, which was then filtered through 60-mesh and 200-mesh filter cloths to obtain the Ganoderma lucidum fermentation extracellular fluid. The basic culture medium included 30 g / L anhydrous glucose, 3 g / L FM801 active dry yeast powder, 2 g / L potassium dihydrogen phosphate, and 2 g / L magnesium sulfate heptahydrate. The sterilization conditions were 121℃ for 30 min.

[0040] (2) Mix hesperidin and Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:75, heat to 70℃, keep warm for 20 min, add 20 times the mass of the mixture of 0.65% hyaluronic acid solution, stir evenly at 80 rpm, and pass through a 150 mesh sieve to obtain a high-efficiency hydrating factor.

[0041] (3) Place pure water in an emulsification pot, heat to 70°C, add high-efficiency moisturizing factor and humectant, stir at 35 rpm for 8 min, add compound preservative, and continue stirring for 40 min to obtain high-efficiency moisturizing toner; the mass ratio of polyaminopropyl biguanide, cetyl chloride and capryloyl hydroxamic acid in the compound preservative is 25:4:8.5; the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the humectant is 4:3:0.4; the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 75:8:4:0.8.

[0042] Comparative Example 2

[0043] (1) Mix hesperidin and anhydrous ethanol at a mass ratio of 1:12, sonicate at 65 kHz for 9 min, then add 3 times the mass of hesperidin Tween 20 and 0.5 times the mass of hesperidin collagen, continue sonicating for 6 min, and pass through a 150 mesh sieve to obtain a high-efficiency moisturizing factor.

[0044] (2) Place pure water in an emulsification pot, heat to 70°C, add high-efficiency moisturizing factor and humectant, stir at 35 rpm for 8 min, add compound preservative, and continue stirring for 40 min to obtain high-efficiency moisturizing toner; the mass ratio of polyaminopropyl biguanide, cetyl chloride and capryloyl hydroxamic acid in the compound preservative is 25:4:8.5; the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the humectant is 4:3:0.4; the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 75:8:4:0.8.

[0045] Comparative Example 3

[0046] (1) Sterilize a 3.5% Ganoderma lucidum powder solution at 121℃ for 35 min, inoculate it into the basal culture medium, sterilize it again, and then take a 0.5 cm sample from the culture plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a primary seed culture. The primary seed culture was then inoculated into the basal medium at a concentration of 10%, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a secondary seed culture. The secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain the fermentation broth, which was then filtered through 60-mesh and 200-mesh filter cloths to obtain the Ganoderma lucidum fermentation extracellular fluid. The basic culture medium included 30 g / L anhydrous glucose, 3 g / L FM801 active dry yeast powder, 2 g / L potassium dihydrogen phosphate, and 2 g / L magnesium sulfate heptahydrate. The sterilization conditions were 121℃ for 30 min.

[0047] (2) Mix hesperidin and anhydrous ethanol at a mass ratio of 1:12, sonicate at 65 kHz for 9 min, then add 3 times the mass of hesperidin Tween 20 and 0.5 times the mass of hesperidin collagen, continue sonicating for 6 min, and pass through a 150 mesh sieve to obtain a mixture; mix the mixture with Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:75, heat to 70℃, keep warm for 20 min, then add 20 times the mass of the mixture of 0.65% hyaluronic acid solution, stir evenly at 80 rpm, and pass through a 150 mesh sieve to obtain a high-efficiency moisturizing factor;

[0048] (3) Place pure water in an emulsification pot, heat it to 70°C, add high-efficiency moisturizing factor and humectant, stir at 35 rpm for 8 min, add compound preservative, and continue stirring for 40 min to obtain high-efficiency moisturizing toner; the mass ratio of polyaminopropyl biguanide and cetylpyridinium chloride in the compound preservative is 25:4; the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the humectant is 4:3:0.4; the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 75:8:4:0.8.

[0049] Comparative Example 4

[0050] (1) Sterilize a 3.5% Ganoderma lucidum powder solution at 121℃ for 35 min, inoculate it into the basal culture medium, sterilize it again, and then take a 0.5 cm sample from the culture plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a primary seed culture. The primary seed culture was then inoculated into the basal medium at a concentration of 10%, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a secondary seed culture. The secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain the fermentation broth, which was then filtered through 60-mesh and 200-mesh filter cloths to obtain the Ganoderma lucidum fermentation extracellular fluid. The basic culture medium included 30 g / L anhydrous glucose, 3 g / L FM801 active dry yeast powder, 2 g / L potassium dihydrogen phosphate, and 2 g / L magnesium sulfate heptahydrate. The sterilization conditions were 121℃ for 30 min.

[0051] (2) Mix hesperidin and anhydrous ethanol at a mass ratio of 1:12, sonicate at 65 kHz for 9 min, then add 3 times the mass of hesperidin Tween 20 and 0.5 times the mass of hesperidin collagen, continue sonicating for 6 min, and pass through a 150 mesh sieve to obtain a mixture; mix the mixture with Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:75, heat to 70℃, keep warm for 20 min, then add 20 times the mass of the mixture of 0.65% hyaluronic acid solution, stir evenly at 80 rpm, and pass through a 150 mesh sieve to obtain a high-efficiency moisturizing factor;

[0052] (3) Place pure water in an emulsification pot, heat it to 70°C, add high-efficiency moisturizing factor and humectant, stir at 35 rpm for 8 min, add compound preservative, and continue stirring for 40 min to obtain high-efficiency moisturizing toner; the mass ratio of polyaminopropyl biguanide and capryloyl hydroxamic acid in the compound preservative is 25:8.5; the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the humectant is 4:3:0.4; the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 75:8:4:0.8.

[0053] Comparative Example 5

[0054] (1) Sterilize a 3.5% Ganoderma lucidum powder solution at 121℃ for 35 min, inoculate it into the basal culture medium, sterilize it again, and then take a 0.5 cm sample from the culture plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a primary seed culture. The primary seed culture was then inoculated into the basal medium at a concentration of 10%, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain a secondary seed culture. The secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 8 days to obtain the fermentation broth, which was then filtered through 60-mesh and 200-mesh filter cloths to obtain the Ganoderma lucidum fermentation extracellular fluid. The basic culture medium included 30 g / L anhydrous glucose, 3 g / L FM801 active dry yeast powder, 2 g / L potassium dihydrogen phosphate, and 2 g / L magnesium sulfate heptahydrate. The sterilization conditions were 121℃ for 30 min.

[0055] (2) Mix hesperidin and anhydrous ethanol at a mass ratio of 1:12, sonicate at 65 kHz for 9 min, then add 3 times the mass of hesperidin Tween 20 and 0.5 times the mass of hesperidin collagen, continue sonicating for 6 min, and pass through a 150 mesh sieve to obtain a mixture; mix the mixture with Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:75, heat to 70℃, keep warm for 20 min, then add 20 times the mass of the mixture of 0.65% hyaluronic acid solution, stir evenly at 80 rpm, and pass through a 150 mesh sieve to obtain a high-efficiency moisturizing factor;

[0056] (3) Place pure water in an emulsification pot, heat it to 70°C, add the high-efficiency moisturizing factor and humectant, stir at 35 rpm for 8 min, add the compound preservative, and continue stirring for 40 min to obtain the high-efficiency moisturizing toner; the mass ratio of cetylpyridinium chloride and capryloyl hydroxamic acid in the compound preservative is 4:8.5; the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the humectant is 4:3:0.4; the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 75:8:4:0.8.

[0057] Example of effect

[0058] Table 1 below presents the performance analysis results of the highly effective moisturizing toners from Examples 1 to 3 and Comparative Examples 1 to 5 of the present invention:

[0059] Table 1

[0060]

[0061] By comparing the experimental data of the examples and comparative examples in Table 1, it can be clearly found that the high-efficiency moisturizing toners prepared in Examples 1, 2 and 3 have better antioxidant and antibacterial properties.

[0062] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No markings in the claims should be construed as limiting the scope of the claims.

Claims

1. A highly effective moisturizing toner, characterized in that, The highly effective moisturizing toner includes purified water, moisturizer, highly effective moisturizing factor, and compound preservative; The highly effective moisturizing factor is prepared by mixing hesperidin and collagen and then placing them in the extracellular fluid after Ganoderma lucidum fermentation for micellar solubilization. The preparation method of the high-efficiency hydrating factor is as follows: (1) Sterilize a 3-4% Ganoderma lucidum powder solution at 121℃ for 30-40 min, inoculate it into the basic culture medium, sterilize it again, and take 0.5 cm from the inoculum plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7-10 days to obtain primary seed culture. The primary seed culture was then inoculated at 10% into the basal medium, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7-10 days to prepare a secondary seed culture. This secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate... 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7-10 days to obtain fermentation broth, which was then filtered through 60 mesh and 200 mesh filter cloths to obtain Ganoderma lucidum fermentation extracellular fluid; (2) Hesperidin and anhydrous ethanol were mixed at a mass ratio of 1:8 to 1:16 and ultrasonically dispersed at 50-80 kHz for 8-10 minutes. Then, 2-4 times the mass of hesperidin Tween 20 and 0.4-0.6 times the mass of hesperidin collagen were added, and ultrasonication was continued for 5 minutes. After stirring for 8 minutes and passing through a 100-200 mesh sieve, a mixture is obtained. The mixture is then mixed with the Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:50 to 1:

100. The mixture is heated to 60-80℃ and kept at that temperature for 10-30 minutes. Then, 10-30 times the mass of the mixture is added to a hyaluronic acid solution with a mass fraction of 0.5-0.8%. The mixture is stirred evenly at 600-1000 rpm and passed through a 100-200 mesh sieve to obtain a highly effective hydrating factor. The composite preservative includes polyurethane biguanide, cetylpyridinium chloride, and octanoyl hydroxamic acid; The moisturizers include glycerin, 1,2-hexanediol, and hyaluronic acid.

2. A method for preparing a highly effective moisturizing toner, characterized in that, The preparation method of the highly effective moisturizing toner includes the following specific steps: (1) Sterilize a 3-4% Ganoderma lucidum powder solution at 121℃ for 30-40 min, inoculate it into the basal culture medium, sterilize it again, and take a 0.5 cm sample from the culture plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7-10 days to obtain a primary seed culture solution. The primary seed culture solution was then inoculated at 10% into the basal medium, and 0.5 cm samples were taken from the bacterial plate. 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7-10 days to obtain a secondary seed culture. This secondary seed culture was then inoculated into the basal medium at a concentration of 10%. A 0.5 cm sample was taken from the bacterial plate... 2 The activated bacterial blocks were cultured at 150 rpm and 26℃ for 7-10 days to obtain the fermentation broth, which was then filtered through 60-mesh and 200-mesh filter cloths to obtain the Ganoderma lucidum fermentation extracellular fluid. (2) Mix hesperidin and anhydrous ethanol at a mass ratio of 1:8 to 1:16, and sonicate at 50 to 80 kHz for 8 to 10 minutes. Then add 2 to 4 times the mass of hesperidin, Tween 20 and 0.4 to 0.6 times the mass of hesperidin, continue sonicating for 5 to 8 minutes, and pass through a 100 to 200 mesh sieve to obtain a mixture. Mix the mixture with Ganoderma lucidum fermentation extracellular fluid at a mass ratio of 1:50 to 1:100, heat to 60 to 80°C, keep warm for 10 to 30 minutes, add 10 to 30 times the mass of the mixture, and stir evenly at 600 to 1000 rpm. Then pass through a 100 to 200 mesh sieve to obtain a high-efficiency moisturizing factor. (3) Place purified water in an emulsification pot, heat it to 60~80℃, add high-efficiency moisturizing factor and moisturizer, stir at 20~50rpm for 5~10min, add compound preservative, and continue stirring for 30~50min to obtain high-efficiency moisturizing toner.

3. The method for preparing a highly effective moisturizing toner according to claim 2, characterized in that, In step (1) above: the basic culture medium includes 30 g / L anhydrous glucose, 3 g / L FM801 active dry yeast powder, 2 g / L potassium dihydrogen phosphate, and 2 g / L magnesium sulfate heptahydrate. The sterilization conditions are 121℃ for 30 min.

4. The method for preparing a highly effective moisturizing toner according to claim 2, characterized in that, In step (3) above: the mass ratio of polyurethane propyl biguanide, cetyl chloride and octanoyl hydroxamic acid in the composite preservative is 15:2:8~30:6:

9.

5. The method for preparing a highly effective moisturizing toner according to claim 2, characterized in that, In step (3) above: the mass ratio of glycerin, 1,2-hexanediol and hyaluronic acid in the moisturizer is 2:2:0.3~5:4:0.

5.

6. The method for preparing a highly effective moisturizing toner according to claim 2, characterized in that, In step (3) above, the mass ratio of purified water, humectant, high-efficiency moisturizing factor and compound preservative is 70:7:3:0.5~80:10:5:1.

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