A kind of hydrating and anti-oxidation essence water and preparation method thereof

Abstract

The invention relates to the technical field of methods for preparing essence water, and in particular to a hydrating and anti-oxidative essence water and a preparation method thereof. The invention uses trypsin and alkaline protease to compound and then enzymolyze goat milk and goat placenta, and after enzymolysis, oligopeptide molecules and active polypeptides with high contents can be obtained. The oligopeptide molecules can repair skin mucosa and can also remove free radicals to achieve an antioxidant effect. The active polypeptides can also play a role in slowing down skin aging on the basis of having an antioxidant effect. The polydopamine in the polydopamine-modified oxidized hyaluronic acid of the invention has good loading capacity, can load the oligopeptide molecules and active polypeptides in the essence water, and form a repair film on the skin surface, thereby assisting the oligopeptide molecules and active polypeptides to play an antioxidant role. The oxidized hyaluronic acid has good moisturizing effect, and the skin can better absorb the oligopeptide molecules and active polypeptides under the condition of good moisturizing effect.

Description

Technical Field

[0001] The invention relates to the technical field of essence water preparation methods, and in particular to a hydrating and anti-oxidative essence water and a preparation method thereof. Background Art

[0002] Skin is an important barrier for the human body to defend against external damage. However, changes and stimulations from the external climate environment can lead to abnormal secretion of the skin's sebaceous glands and sweat glands. As human skin ages, sebum secretion decreases, and the skin's ability to retain moisture decreases, which causes the skin to become dry, the stratum corneum of the skin's epidermis to thicken, and problems such as skin keratinization, cracking, and rough skin surface occur. In recent years, people have paid more and more attention to skin care. Skin care products are essential for daily beauty and skin care. They can fully replenish the moisture in the stratum corneum of the skin and can be used to maintain the skin and keep it healthy.

[0003] With the improvement of modern living standards, people have more and more demands for skin care products. However, in recent years, some safety issues have also emerged in the skin care industry, and people's attention to this aspect has gradually increased. Common skin care essence water contains more ethanol and other substances that are irritating to the skin, which can easily cause skin allergies during actual use. In addition, many skin care essence waters only use plant flavonoids or polysaccharides as the main antioxidant ingredients, and the skin care effect is relatively simple, and the antioxidant effect is not obvious enough. Therefore, how to prepare an essence water with safe ingredients and good antioxidant skin care effect has become a technical problem that needs to be solved urgently by technicians in this field. Summary of the invention

[0004] Technical issues solved

[0005] In view of the above-mentioned shortcomings of the prior art, the present invention provides a hydrating and antioxidant essence water and a preparation method thereof, aiming to make the prepared hydrating and antioxidant essence water have better hydrating, moisturizing and antioxidant effects.

[0006] Technical Solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] A hydrating and anti-oxidant essence water, the raw material components of the hydrating and anti-oxidant essence water include: an antioxidant peptide component, polydopamine-modified oxidized hyaluronic acid and a homemade wax gourd polysaccharide solution;

[0009] The antioxidant peptide component is prepared by enzymatic hydrolysis of goat milk and goat placenta as raw materials;

[0010] The polydopamine-modified oxidized hyaluronic acid is prepared by modifying homemade oxidized hyaluronic acid with polymerized dopamine;

[0011] The self-made wax gourd polysaccharide solution is prepared by taking wax gourd as a raw material and then subjecting it to ultrasonic extraction.

[0012] Furthermore, the preparation steps of the antioxidant peptide component are:

[0013] Step a, first freeze-drying goat milk to prepare goat milk freeze-dried powder for standby use, then using physiological saline to wash the sheep placenta, cutting it into pieces to remove fascia and blood vessels, pouring it into a homogenizer, adding physiological saline with a mass of 9 times that of the placenta, and crushing it into a homogenate, repeatedly freezing and thawing the homogenate 3 times, and then centrifuging it at a speed of 5000-6000r / min for 2-3min, collecting the precipitate and freeze-drying it to prepare the sheep placenta freeze-dried powder, wherein the freeze-drying temperature is -20°C;

[0014] Step b, mixing the goat milk freeze-dried powder and the goat placenta freeze-dried powder in step a in an equal weight ratio, pouring the mixture into a grinder, grinding it and passing it through a 50-60 mesh sieve, then mixing it with deionized water in a solid-liquid ratio of 1:20 g / mL, stirring it at a stirring speed of 300-400 r / min for 5-6 minutes, and the result is recorded as a mixed component;

[0015] Step c, heating the mixed components in step b to 85-90° C., keeping the temperature for 2-3 minutes, then cooling to 55-56° C., adjusting the pH value to 6.5, adding the complex enzyme component according to the enzyme addition amount of 1-2%, reacting at a constant temperature for 100-120 minutes, and then boiling water bathing for 15-20 minutes to inactivate the enzyme, cooling to room temperature, adjusting the pH value to 7 again, and centrifuging at a speed of 8000 r / min for 4-5 minutes, collecting the supernatant and freeze-drying it into powder, crushing it through a 100-120 mesh sieve, and obtaining the antioxidant peptide component, wherein the freeze-drying temperature is -30° C.

[0016] Furthermore, in step c, a hydrochloric acid solution with a concentration of 0.1 mol / L is used to adjust the pH value.

[0017] Furthermore, the composite enzyme component in step c is prepared by mixing trypsin and alkaline protease in an equal weight ratio.

[0018] Furthermore, the preparation method of the homemade oxidized hyaluronic acid is: weigh 4-5 parts by weight of hyaluronic acid and dissolve it in 400-500 parts by weight of deionized water, add 2-3 parts by weight of sodium periodate and stir for 2-3 hours at a stirring speed of 300-400r / min under light-proof conditions to react, then add 4-5 parts by weight of polyethylene glycol and pour it into a dialysis bag with a molecular weight cutoff of 3500 and dialyze it in pure water for 2-3 days, and the obtained homemade oxidized hyaluronic acid is obtained after freeze-drying, wherein the freeze-drying temperature is -20°C.

[0019] Furthermore, the preparation steps of the polydopamine-modified oxidized hyaluronic acid are as follows:

[0020] Step 1, first weigh 1-2 parts by weight of dopamine and dissolve it in 80-100 parts by weight of deionized water, stir it at a stirring speed of 500-600 r / min for 5-6 minutes to prepare a dopamine solution, then weigh 1-2 parts by weight of sodium periodate and dissolve it in 20-30 parts by weight of deionized water, stir it at a stirring speed of 200-300 r / min for 3-5 minutes to prepare a sodium periodate solution;

[0021] Step 2, after heating the dopamine solution in step 1 to 25° C. in a water bath, pour the sodium periodate solution in step 1 into the solution under stirring at 400-500 r / min for 8-9 hours, centrifuge at 12000 r / min for 10 minutes after the reaction is completed, remove the supernatant, add deionized water for washing and centrifuge again, repeat 4-5 times, and the resulting precipitate is recorded as polydopamine;

[0022] Step 3, weigh 8-10 parts by weight of the homemade oxidized hyaluronic acid and 1-2 parts by weight of the polydopamine in step 2 and dissolve them in 500-600 parts by weight of deionized water. After fully dissolved, adjust the pH value to 5, stir at a stirring speed of 300-400 r / min for 8-10 hours under light-proof conditions to react, place in a vacuum drying oven after the reaction is completed, grind and crush through a 200-mesh sieve, and the obtained product is polydopamine-modified oxidized hyaluronic acid.

[0023] Furthermore, in step 3, the pH value is adjusted using a hydrochloric acid solution with a concentration of 0.2 mol / L.

[0024] Furthermore, the preparation method of the homemade wax gourd polysaccharide solution is as follows: peel the wax gourd, clean it, cut it into pieces and freeze-dry it, grind it into powder and mix it with deionized water at a solid-liquid ratio of 1:20g / mL, extract it with ultrasound and centrifuge it at a speed of 5000-6000r / min for 5-8min, concentrate the supernatant to 1 / 10 of its original volume by rotary evaporation, pour it into anhydrous ethanol with a volume 10 times that of the original volume and precipitate it under stirring, centrifuge it at a speed of 5000r / min for 5-8min after precipitation, dissolve the precipitate in deionized water with a volume 8-10 times that of the original volume, and the result is the homemade wax gourd polysaccharide solution.

[0025] Furthermore, the stirring speed of the stirring condition is 500-600r / min.

[0026] A method for preparing a hydrating and antioxidant essence water, wherein the raw material components of the hydrating and antioxidant essence water further include: squalane, vitamin E, glycerin, potassium sorbate and deionized water;

[0027] The preparation method of the hydrating and antioxidant essence water is as follows:

[0028] S1, weighing 0.1-0.2 parts by weight of squalane, 0.1-0.2 parts by weight of vitamin E and 5-8 parts by weight of glycerol, and mixing them evenly to obtain the glycerol component;

[0029] S2, weigh 2-3 parts by weight of antioxidant peptide component and 1-2 parts by weight of polydopamine-modified oxidized hyaluronic acid, pour into 45-50 parts by weight of deionized water, stir at a stirring speed of 400-500r / min for 5-8min in a water bath at 45-50°C, then add 25-28 parts by weight of homemade wax gourd polysaccharide solution and 0.03-0.05 parts by weight of potassium sorbate and continue stirring for 4-5min, the result is recorded as the mixed solution component;

[0030] S3. Pour the glycerin component in S1 and the mixed liquid component in S2 into an emulsifying homogenizer for emulsification and homogenization. Use citric acid or triethanolamine to adjust the pH value to 6.0-6.5, and the resulting essence water is hydrating and antioxidant.

[0031] Beneficial Effects

[0032] The present invention provides a hydrating and anti-oxidative essence water and a preparation method thereof. Compared with the prior art, the present invention has the following beneficial effects:

[0033] The present invention uses trypsin and alkaline protease to compound and then enzymolyze goat milk and goat placenta, and after enzymolysis, oligopeptide molecules and active polypeptides with high content can be obtained, wherein the oligopeptide molecules can repair skin mucosa, and can also remove free radicals to achieve an antioxidant effect, and the active polypeptides can also play a role in slowing down skin aging on the basis of having an antioxidant effect; the present invention first polymerizes dopamine into polydopamine by oxidative polymerization, then oxidizes hyaluronic acid to obtain self-made oxidized hyaluronic acid, and finally uses polydopamine to modify the self-made oxidized hyaluronic acid to obtain polydopamine-modified oxidized hyaluronic acid, wherein the polydopamine has a good loading capacity, can load the oligopeptide molecules and active polypeptides in the essence water, and form a repair film on the skin surface, thereby assisting the oligopeptide molecules and active polypeptides to play an antioxidant effect, and the oxidized hyaluronic acid has a good moisturizing effect, and the skin can better absorb the oligopeptide molecules and active polypeptides under the condition of a good moisturizing effect, thereby enhancing the antioxidant performance of the essence water to a certain extent. The hydrating and antioxidant essence water prepared by the present invention is added with polysaccharide components extracted from wax gourd, which can have antioxidant and hydrating and moisturizing effects. In addition, the cost of wax gourd is relatively low, which can reduce the cost of the hydrating and antioxidant essence water in the present invention to a certain extent, making it have better market application value. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] The present invention will be further described below in conjunction with the embodiments.

[0036] Example 1

[0037] A hydrating and antioxidant essence water in this embodiment, the raw material components of the hydrating and antioxidant essence water include: antioxidant peptide components, polydopamine-modified oxidized hyaluronic acid and homemade wax gourd polysaccharide solution;

[0038] The antioxidant peptide component is made from goat milk and goat placenta through enzymatic hydrolysis;

[0039] Polydopamine-modified oxidized hyaluronic acid is prepared by modifying self-made oxidized hyaluronic acid with polymerized dopamine;

[0040] The homemade wax gourd polysaccharide solution was prepared from wax gourd as raw material after ultrasonic extraction.

[0041] The preparation steps of the antioxidant peptide component are as follows:

[0042] Step a, first freeze-drying goat milk to prepare goat milk freeze-dried powder for standby use, then using physiological saline to wash the sheep placenta, cutting it into pieces to remove fascia and blood vessels, pouring it into a homogenizer, adding physiological saline 9 times its mass, and crushing it into a homogenate, repeatedly freezing and thawing the homogenate 3 times, and then centrifuging it at a speed of 5000r / min for 2min, collecting the precipitate and freeze-drying it to prepare the sheep placenta freeze-dried powder, wherein the freeze-drying temperature is -20°C;

[0043] Step b, mixing the goat milk freeze-dried powder and the goat placenta freeze-dried powder in step a in an equal weight ratio, pouring the mixture into a grinder, grinding and passing through a 50-mesh sieve, then mixing with deionized water in a solid-liquid ratio of 1:20 g / mL, stirring at a stirring speed of 300 r / min for 5 minutes, and recording the result as a mixed component;

[0044] Step c, heating the mixed components in step b to 85° C., keeping the temperature for 2 minutes, then cooling to 55° C., adjusting the pH value to 6.5, adding the complex enzyme component according to 1% of the enzyme addition amount, reacting at a constant temperature for 100 minutes, and then boiling water bathing for 15 minutes to inactivate the enzyme, cooling to room temperature, adjusting the pH value to 7 again, and centrifuging at a speed of 8000 r / min for 4 minutes, collecting the supernatant and freeze-drying it into powder, crushing it through a 100-mesh sieve, and obtaining the antioxidant peptide component, wherein the freeze-drying temperature is -30° C.

[0045] In step c, the pH value is adjusted using a hydrochloric acid solution with a concentration of 0.1 mol / L.

[0046] The composite enzyme component in step c is prepared by mixing trypsin and alkaline protease in an equal weight ratio.

[0047] The preparation method of the homemade oxidized hyaluronic acid is as follows: 4 parts by weight of hyaluronic acid is weighed and dissolved in 400 parts by weight of deionized water, 2 parts by weight of sodium periodate is added, and the mixture is stirred at a stirring speed of 300 r / min for 2 hours under light-proof conditions for reaction, then 4 parts by weight of polyethylene glycol is added and poured into a dialysis bag with a molecular weight cutoff of 3500 and dialyzed in pure water for 2 days, and the homemade oxidized hyaluronic acid is obtained after freeze-drying, wherein the freeze-drying temperature is -20°C.

[0048] The preparation steps of polydopamine-modified oxidized hyaluronic acid are as follows:

[0049] Step 1, first weigh 1 part by weight of dopamine and dissolve it in 80 parts by weight of deionized water, stir it at a stirring speed of 500 r / min for 5 minutes to prepare a dopamine solution, then weigh 1 part by weight of sodium periodate and dissolve it in 20 parts by weight of deionized water, stir it at a stirring speed of 200 r / min for 3 minutes to prepare a sodium periodate solution;

[0050] Step 2, after heating the dopamine solution in step 1 to 25° C. in a water bath, pour the sodium periodate solution in step 1 into the solution under stirring at 400 r / min for 8 h, centrifuge at 12000 r / min for 10 min after the reaction is completed, remove the supernatant, add deionized water for washing and centrifuge again, repeat 4 times, and the resulting precipitate is recorded as polydopamine;

[0051] Step 3, weigh 8 parts by weight of the homemade oxidized hyaluronic acid and 1 part by weight of the polydopamine in step 2 and dissolve them in 500 parts by weight of deionized water. After fully dissolved, adjust the pH value to 5, stir at a stirring speed of 300 r / min for 8 hours under light-proof conditions to react, place in a vacuum drying oven after the reaction is completed, grind and crush through a 200-mesh sieve, and the obtained product is polydopamine-modified oxidized hyaluronic acid.

[0052] In step 3, the pH value is adjusted using a hydrochloric acid solution with a concentration of 0.2 mol / L.

[0053] The preparation method of the homemade wax gourd polysaccharide solution is as follows: peel the wax gourd, clean it, cut it into pieces and freeze-dry it, grind it into powder and mix it with deionized water at a solid-liquid ratio of 1:20g / mL, extract it with ultrasound and centrifuge it at a speed of 5000r / min for 5min, concentrate the supernatant to 1 / 10 of its original volume by rotary evaporation, pour it into anhydrous ethanol with a volume of 10 times that of the original volume and precipitate it under stirring, centrifuge it at a speed of 5000r / min for 5min after precipitation, dissolve the precipitate in deionized water with a volume of 8 times that of the original volume, and the result is the homemade wax gourd polysaccharide solution.

[0054] The stirring speed of the stirring condition was 500 r / min.

[0055] A method for preparing a hydrating and antioxidant essence water, wherein the raw material components of the hydrating and antioxidant essence water further include: squalane, vitamin E, glycerin, potassium sorbate and deionized water;

[0056] The preparation method of the hydrating and antioxidant essence water is as follows:

[0057] S1, weighing 0.1 parts by weight of squalane, 0.1 parts by weight of vitamin E and 5 parts by weight of glycerol, mixing them evenly and recording them as the glycerol component;

[0058] S2, weighing 2 parts by weight of antioxidant peptide component and 1 part by weight of polydopamine-modified oxidized hyaluronic acid, pouring into 45 parts by weight of deionized water, stirring at a stirring speed of 400 r / min for 5 minutes in a water bath at 45° C., then adding 25 parts by weight of homemade wax gourd polysaccharide solution and 0.03 parts by weight of potassium sorbate and continuing stirring for 4 minutes, the result is recorded as the mixed solution component;

[0059] S3. Pour the glycerin component in S1 and the mixed liquid component in S2 into an emulsifying homogenizer for emulsification and homogenization. Use citric acid or triethanolamine to adjust the pH value to 6.0, and the resulting essence water is hydrating and antioxidant.

[0060] Example 2

[0061] A hydrating and antioxidant essence water in this embodiment, the raw material components of the hydrating and antioxidant essence water include: antioxidant peptide components, polydopamine-modified oxidized hyaluronic acid and homemade wax gourd polysaccharide solution;

[0062] The antioxidant peptide component is made from goat milk and goat placenta through enzymatic hydrolysis;

[0063] Polydopamine-modified oxidized hyaluronic acid is prepared by modifying self-made oxidized hyaluronic acid with polymerized dopamine;

[0064] The homemade wax gourd polysaccharide solution was prepared from wax gourd as raw material after ultrasonic extraction.

[0065] The preparation steps of the antioxidant peptide component are as follows:

[0066] Step a, first freeze-drying goat milk to prepare goat milk freeze-dried powder for standby use, then using physiological saline to wash the sheep placenta, cutting it into pieces to remove fascia and blood vessels, pouring it into a homogenizer, adding physiological saline 9 times its mass, and crushing it into a homogenate, repeatedly freezing and thawing the homogenate 3 times, and then centrifuging it at a speed of 6000r / min for 3min, collecting the precipitate and freeze-drying it to prepare the sheep placenta freeze-dried powder, wherein the freeze-drying temperature is -20°C;

[0067] Step b, mixing the goat milk freeze-dried powder and the goat placenta freeze-dried powder in step a in an equal weight ratio, pouring the mixture into a grinder, grinding it and passing it through a 60-mesh sieve, then mixing it with deionized water in a solid-liquid ratio of 1:20 g / mL, stirring it at a stirring speed of 400 r / min for 6 minutes, and the result is recorded as a mixed component;

[0068] Step c, heating the mixed components in step b to 90° C., keeping warm for 3 minutes, then cooling to 56° C., adjusting the pH value to 6.5, adding the complex enzyme component according to 2% of the enzyme addition amount, reacting at a constant temperature for 120 minutes, and then boiling in a water bath for 20 minutes to inactivate the enzyme, cooling to room temperature, adjusting the pH value to 7 again, and centrifuging at a speed of 8000 r / min for 5 minutes, collecting the supernatant and freeze-drying it into powder, crushing it through a 120-mesh sieve, and obtaining the antioxidant peptide component, wherein the freeze-drying temperature is -30° C.

[0069] In step c, the pH value is adjusted using a hydrochloric acid solution with a concentration of 0.1 mol / L.

[0070] The composite enzyme component in step c is prepared by mixing trypsin and alkaline protease in an equal weight ratio.

[0071] The preparation method of the homemade oxidized hyaluronic acid is as follows: 5 parts by weight of hyaluronic acid is weighed and dissolved in 500 parts by weight of deionized water, 3 parts by weight of sodium periodate is added, and the mixture is stirred at a stirring speed of 400 r / min for 3 hours under light-proof conditions for reaction, then 5 parts by weight of polyethylene glycol is added and poured into a dialysis bag with a molecular weight cutoff of 3500 and dialyzed in pure water for 3 days, and the homemade oxidized hyaluronic acid is obtained after freeze-drying, wherein the freeze-drying temperature is -20°C.

[0072] The preparation steps of polydopamine-modified oxidized hyaluronic acid are as follows:

[0073] Step 1, first weigh 2 parts by weight of dopamine and dissolve it in 100 parts by weight of deionized water, stir it at a stirring speed of 600 r / min for 6 minutes to prepare a dopamine solution, then weigh 2 parts by weight of sodium periodate and dissolve it in 30 parts by weight of deionized water, stir it at a stirring speed of 300 r / min for 5 minutes to prepare a sodium periodate solution;

[0074] Step 2, after heating the dopamine solution in step 1 to 25°C in a water bath, pour the sodium periodate solution in step 1 into the solution under stirring at 500 r / min for 9 hours, centrifuge at 12000 r / min for 10 minutes after the reaction is completed, remove the supernatant, add deionized water for washing and centrifuge again, repeat 5 times, and the resulting precipitate is recorded as polydopamine;

[0075] Step 3, weigh 10 parts by weight of the homemade oxidized hyaluronic acid and 2 parts by weight of the polydopamine in step 2 and dissolve them in 600 parts by weight of deionized water. After fully dissolved, adjust the pH value to 5, stir at a stirring speed of 400 r / min for 10 hours under light-proof conditions to react, place in a vacuum drying oven after the reaction is completed, grind and crush through a 200-mesh sieve, and the obtained product is polydopamine-modified oxidized hyaluronic acid.

[0076] In step 3, the pH value is adjusted using a hydrochloric acid solution with a concentration of 0.2 mol / L.

[0077] The preparation method of the homemade wax gourd polysaccharide solution is as follows: peel the wax gourd, clean it, cut it into pieces and freeze-dry it, grind it into powder and mix it with deionized water at a solid-liquid ratio of 1:20g / mL, extract it with ultrasound and centrifuge it at a speed of 6000r / min for 8min, concentrate the supernatant to 1 / 10 of its original volume by rotary evaporation, pour in anhydrous ethanol with a volume 10 times that of the original volume and precipitate it under stirring, centrifuge it at a speed of 5000r / min for 8min after precipitation, dissolve the precipitate in deionized water with a volume 10 times that of the original volume, and the result is the homemade wax gourd polysaccharide solution.

[0078] The stirring speed of the stirring condition was 600 r / min.

[0079] A method for preparing a hydrating and antioxidant essence water, wherein the raw material components of the hydrating and antioxidant essence water further include: squalane, vitamin E, glycerin, potassium sorbate and deionized water;

[0080] The preparation method of the hydrating and antioxidant essence water is as follows:

[0081] S1, weighing 0.2 parts by weight of squalane, 0.2 parts by weight of vitamin E and 8 parts by weight of glycerol, mixing them evenly and recording them as the glycerol component;

[0082] S2, weighing 3 parts by weight of antioxidant peptide component and 2 parts by weight of polydopamine-modified oxidized hyaluronic acid, pouring into 50 parts by weight of deionized water, stirring at a stirring speed of 500 r / min for 8 minutes in a water bath at 50° C., then adding 28 parts by weight of homemade wax gourd polysaccharide solution and 0.05 parts by weight of potassium sorbate and continuing stirring for 5 minutes, the result is recorded as the mixed solution component;

[0083] S3. Pour the glycerin component in S1 and the mixed liquid component in S2 into an emulsifying homogenizer for emulsification and homogenization. Use citric acid or triethanolamine to adjust the pH value to 6.5, and the resulting essence water is hydrating and antioxidant.

[0084] Example 3

[0085] A hydrating and antioxidant essence water in this embodiment, the raw material components of the hydrating and antioxidant essence water include: antioxidant peptide components, polydopamine-modified oxidized hyaluronic acid and homemade wax gourd polysaccharide solution;

[0086] The antioxidant peptide component is made from goat milk and goat placenta through enzymatic hydrolysis;

[0087] Polydopamine-modified oxidized hyaluronic acid is prepared by modifying self-made oxidized hyaluronic acid with polymerized dopamine;

[0088] The homemade wax gourd polysaccharide solution was prepared from wax gourd as raw material after ultrasonic extraction.

[0089] The preparation steps of the antioxidant peptide component are as follows:

[0090] Step a, first freeze-drying goat milk to prepare goat milk freeze-dried powder for standby use, then using physiological saline to wash the sheep placenta, cutting it into pieces to remove fascia and blood vessels, pouring it into a homogenizer, adding physiological saline 9 times its mass, and crushing it into a homogenate, repeatedly freezing and thawing the homogenate 3 times, and then centrifuging it at a speed of 6000r / min for 3min, collecting the precipitate and freeze-drying it to prepare the sheep placenta freeze-dried powder, wherein the freeze-drying temperature is -20°C;

[0091] Step b, mixing the goat milk freeze-dried powder and the goat placenta freeze-dried powder in step a in an equal weight ratio, pouring the mixture into a grinder, grinding and passing through a 50-mesh sieve, then mixing with deionized water in a solid-liquid ratio of 1:20 g / mL, stirring at a stirring speed of 400 r / min for 6 minutes, and recording the result as a mixed component;

[0092] Step c, heating the mixed components in step b to 88° C., keeping warm for 2 minutes, then cooling to 56° C., adjusting the pH value to 6.5, adding the complex enzyme component according to 2% of the enzyme addition amount, reacting at a constant temperature for 110 minutes, and then boiling in a water bath for 18 minutes to inactivate the enzyme, cooling to room temperature, adjusting the pH value to 7 again, and centrifuging at a speed of 8000 r / min for 5 minutes, collecting the supernatant and freeze-drying it into powder, crushing it through a 100-mesh sieve, and obtaining the antioxidant peptide component, wherein the freeze-drying temperature is -30° C.

[0093] In step c, the pH value is adjusted using a hydrochloric acid solution with a concentration of 0.1 mol / L.

[0094] The composite enzyme component in step c is prepared by mixing trypsin and alkaline protease in an equal weight ratio.

[0095] The preparation method of the homemade oxidized hyaluronic acid is as follows: 5 parts by weight of hyaluronic acid is weighed and dissolved in 400 parts by weight of deionized water, 3 parts by weight of sodium periodate is added, and the mixture is stirred at a stirring speed of 300 r / min for 2 hours under light-proof conditions for reaction, then 5 parts by weight of polyethylene glycol is added and poured into a dialysis bag with a molecular weight cutoff of 3500 and dialyzed in pure water for 3 days, and the homemade oxidized hyaluronic acid is obtained after freeze-drying, wherein the freeze-drying temperature is -20°C.

[0096] The preparation steps of polydopamine-modified oxidized hyaluronic acid are as follows:

[0097] Step 1, first weigh 2 parts by weight of dopamine and dissolve it in 90 parts by weight of deionized water, stir it at a stirring speed of 500 r / min for 6 minutes to prepare a dopamine solution, then weigh 2 parts by weight of sodium periodate and dissolve it in 25 parts by weight of deionized water, stir it at a stirring speed of 200 r / min for 4 minutes to prepare a sodium periodate solution;

[0098] Step 2, after heating the dopamine solution in step 1 to 25° C. in a water bath, pour the sodium periodate solution in step 1 into the solution under stirring at 500 r / min for 8 h, centrifuge at 12000 r / min for 10 min after the reaction is completed, remove the supernatant, add deionized water for washing and centrifuge again, repeat 4 times, and the resulting precipitate is recorded as polydopamine;

[0099] Step 3, weigh 9 parts by weight of the homemade oxidized hyaluronic acid and 2 parts by weight of the polydopamine in step 2 and dissolve them in 500 parts by weight of deionized water. After fully dissolved, adjust the pH value to 5, stir at a stirring speed of 300 r / min for 9 hours under light-proof conditions to react, and after the reaction is completed, place it in a vacuum drying oven to dry, grind it and pass it through a 200-mesh sieve to obtain polydopamine-modified oxidized hyaluronic acid.

[0100] In step 3, the pH value is adjusted using a hydrochloric acid solution with a concentration of 0.2 mol / L.

[0101] The preparation method of the homemade wax gourd polysaccharide solution is as follows: peel the wax gourd, clean it, cut it into pieces and freeze-dry it, grind it into powder and mix it with deionized water at a solid-liquid ratio of 1:20g / mL, extract it with ultrasound and centrifuge it at a speed of 6000r / min for 7min, concentrate the supernatant to 1 / 10 of its original volume by rotary evaporation, pour it into anhydrous ethanol with a volume of 10 times that of the original volume and precipitate it under stirring, centrifuge it at a speed of 5000r / min for 6min after precipitation, dissolve the precipitate in deionized water with a volume of 9 times that of the original volume, and the result is the homemade wax gourd polysaccharide solution.

[0102] The stirring speed of the stirring condition was 600 r / min.

[0103] A method for preparing a hydrating and antioxidant essence water, wherein the raw material components of the hydrating and antioxidant essence water further include: squalane, vitamin E, glycerin, potassium sorbate and deionized water;

[0104] The preparation method of the hydrating and antioxidant essence water is as follows:

[0105] S1, weighing 0.1 parts by weight of squalane, 0.2 parts by weight of vitamin E and 6 parts by weight of glycerol, mixing them evenly and recording them as the glycerol component;

[0106] S2, weighing 2 parts by weight of antioxidant peptide component and 2 parts by weight of polydopamine-modified oxidized hyaluronic acid, pouring into 48 parts by weight of deionized water, stirring at a stirring speed of 400 r / min for 7 minutes in a water bath at 47° C., then adding 26 parts by weight of homemade wax gourd polysaccharide solution and 0.04 parts by weight of potassium sorbate and continuing stirring for 5 minutes, the result is recorded as the mixed solution component;

[0107] S3. Pour the glycerin component in S1 and the mixed liquid component in S2 into an emulsifying homogenizer for emulsification and homogenization. Use citric acid or triethanolamine to adjust the pH value to 6.5, and the resulting essence water is hydrating and antioxidant.

[0108] Comparative Example 1

[0109] The hydrating and antioxidant essence water and its preparation method in this comparative example are substantially the same as those in Example 1, with the main difference being that in this comparative example 1, the goat milk and goat placenta in the antioxidant peptide component raw materials in Example 1 are replaced with a single goat placenta.

[0110] Comparative Example 2

[0111] The hydrating and antioxidant essence water and its preparation method in this comparative example are substantially the same as those in Example 1, with the main difference being that in this comparative example 2, the polydopamine in step 3 of preparing the polydopamine-modified oxidized hyaluronic acid in Example 1 is replaced with unpolymerized dopamine.

[0112] Comparative Example 3

[0113] The hydrating and antioxidant essence water and its preparation method in this comparative example are roughly the same as those in Example 1, with the main difference being that in this comparative example 3, the homemade oxidized hyaluronic acid in step 3 of preparing the polydopamine-modified oxidized hyaluronic acid in Example 1 replaces the unoxidized hyaluronic acid.

[0114] Performance Testing

[0115] The hydrating and antioxidant essence waters prepared in Examples 1-3 were marked as Example 1, Example 2 and Example 3, respectively; the hydrating and antioxidant essence waters prepared in Comparative Examples 1-3 were marked as Comparative Example 1, Comparative Example 2 and Comparative Example 3, respectively; 20 volunteers were randomly selected, and 1 mL of the hydrating and antioxidant essence waters in Examples 1-3 and Comparative Examples 1-3 were applied to the face after cleansing every morning and evening; the facial skin of the volunteers was tested on the 1st day, the 10th day and the 30th day, respectively; the test items included melanin content, stratum corneum water content and skin elasticity, and the obtained data were recorded in Tables 1, 2 and 3, respectively:

[0116]

[0117] Table 1

[0118]

[0119] Table 2

[0120]

[0121] Table 3

[0122] It can be seen from the data in Table 1, Table 2 and Table 3 that the skin condition of the volunteers who applied the moisturizing and antioxidant essence water prepared in Examples 1-3 was significantly better, indicating that the antioxidant skin care effect of the antioxidant peptides extracted from sheep placenta and sheep milk is significantly better than the antioxidant skin care effect of the antioxidant peptides extracted from sheep placenta as a single component, and also indicating that modifying the oxidized hyaluronic acid after polymerization of dopamine can have a better moisturizing and moisturizing effect. In summary, the moisturizing and antioxidant essence water prepared by the present invention has better market application value.

[0123] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0124] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A hydrating and anti-oxidant essence water, characterized in that: The raw material components of the hydrating and anti-oxidation essence water include: antioxidant peptide components, polydopamine-modified oxidized hyaluronic acid and homemade wax gourd polysaccharide solution; The antioxidant peptide component is prepared by enzymatic hydrolysis of goat milk and goat placenta as raw materials; The polydopamine-modified oxidized hyaluronic acid is prepared by modifying homemade oxidized hyaluronic acid with polymerized dopamine; The self-made wax gourd polysaccharide solution is prepared by using wax gourd as a raw material and then ultrasonically extracting it; The preparation steps of the antioxidant peptide component are: Step a, first freeze-drying goat milk to prepare goat milk freeze-dried powder for standby use, then using physiological saline to clean the sheep placenta, cutting it into pieces to remove fascia and blood vessels, pouring it into a homogenizer, adding physiological saline 9 times its mass, and crushing it into a homogenate, repeatedly freezing and thawing the homogenate 3 times, and then centrifuging it at a speed of 5000-6000r / min for 2-3min, collecting the precipitate and freeze-drying it to prepare the sheep placenta freeze-dried powder; Step b, mixing the goat milk freeze-dried powder and the goat placenta freeze-dried powder in step a in an equal weight ratio, pouring the mixture into a grinder, grinding it and passing it through a 50-60 mesh sieve, then mixing it with deionized water in a solid-liquid ratio of 1:20 g / mL, stirring it at a stirring speed of 300-400 r / min for 5-6 minutes, and the result is recorded as a mixed component; Step c, heating the mixed components in step b to 85-90° C., keeping the temperature for 2-3 minutes, then cooling to 55-56° C., adjusting the pH value to 6.5, adding the complex enzyme component according to the enzyme addition amount of 1-2%, reacting at a constant temperature for 100-120 minutes, and then boiling water bathing for 15-20 minutes to kill the enzyme, cooling to room temperature, adjusting the pH value to 7 again, and centrifuging at a speed of 8000 r / min for 4-5 minutes, collecting the supernatant and freeze-drying it into powder, and crushing it through a 100-120 mesh sieve to obtain the antioxidant peptide component; The composite enzyme component in step c is prepared by mixing trypsin and alkaline protease in an equal weight ratio; The preparation method of the self-made oxidized hyaluronic acid is as follows: 4-5 parts by weight of hyaluronic acid is weighed and dissolved in 400-500 parts by weight of deionized water, 2-3 parts by weight of sodium periodate is added, and the mixture is stirred at a stirring speed of 300-400 r / min for 2-3 hours under light-proof conditions for reaction, then 4-5 parts by weight of polyethylene glycol is added, and the mixture is poured into a dialysis bag with a molecular weight cutoff of 3500 and dialyzed in pure water for 2-3 days, and the self-made oxidized hyaluronic acid is obtained after freeze-drying; The preparation steps of the polydopamine-modified oxidized hyaluronic acid are as follows: Step 1, first weigh 1-2 parts by weight of dopamine and dissolve it in 80-100 parts by weight of deionized water, stir it at a stirring speed of 500-600 r / min for 5-6 minutes to prepare a dopamine solution, then weigh 1-2 parts by weight of sodium periodate and dissolve it in 20-30 parts by weight of deionized water, stir it at a stirring speed of 200-300 r / min for 3-5 minutes to prepare a sodium periodate solution; Step 2, after heating the dopamine solution in step 1 to 25° C. in a water bath, pour the sodium periodate solution in step 1 into the solution under stirring at 400-500 r / min for 8-9 hours, centrifuge at 12000 r / min for 10 minutes after the reaction is completed, remove the supernatant, add deionized water for washing and centrifuge again, repeat 4-5 times, and the resulting precipitate is recorded as polydopamine; Step 3, weigh 8-10 parts by weight of the homemade oxidized hyaluronic acid and 1-2 parts by weight of the polydopamine in step 2 and dissolve them in 500-600 parts by weight of deionized water. After fully dissolved, adjust the pH value to 5, stir at a stirring speed of 300-400 r / min for 8-10 hours under light-proof conditions to react, place in a vacuum drying oven after the reaction is completed, grind and crush through a 200-mesh sieve, and the obtained product is polydopamine-modified oxidized hyaluronic acid.

2. The hydrating and antioxidant essence water according to claim 1, characterized in that: The pH value in step c is adjusted using a hydrochloric acid solution with a concentration of 0.1 mol / L.

3. The hydrating and antioxidant essence water according to claim 2, characterized in that: In step 3, the pH value is adjusted using a hydrochloric acid solution with a concentration of 0.2 mol / L.

4. The hydrating and antioxidant essence water according to claim 1, characterized in that: The preparation method of the homemade wax gourd polysaccharide solution is as follows: peel the wax gourd, clean it, cut it into pieces and freeze-dry it, grind it into powder and mix it with deionized water at a solid-liquid ratio of 1:20g / mL, extract it by ultrasonic wave and centrifuge it at a speed of 5000-6000r / min for 5-8min, concentrate the supernatant to 1 / 10 of its original volume by rotary evaporation, pour it into anhydrous ethanol with a volume 10 times that of the original volume and precipitate it under stirring, centrifuge it at a speed of 5000r / min for 5-8min after precipitation, dissolve the precipitate in deionized water with a volume 8-10 times that of the original volume, and obtain the homemade wax gourd polysaccharide solution.

5. The hydrating and antioxidant essence water according to claim 4, characterized in that: The stirring speed of the stirring condition is 500-600r / min.

6. A method for preparing a hydrating and antioxidant essence water according to any one of claims 1 to 5, characterized in that: The raw material components of the hydrating and antioxidant essence water also include: squalane, vitamin E, glycerin, potassium sorbate and deionized water; The preparation method of the hydrating and antioxidant essence water is as follows: S1, weighing 0.1-0.2 parts by weight of squalane, 0.1-0.2 parts by weight of vitamin E and 5-8 parts by weight of glycerol, and mixing them evenly to obtain the glycerol component; S2, weigh 2-3 parts by weight of antioxidant peptide component and 1-2 parts by weight of polydopamine-modified oxidized hyaluronic acid, pour into 45-50 parts by weight of deionized water, stir at a stirring speed of 400-500r / min for 5-8min in a water bath at 45-50°C, then add 25-28 parts by weight of homemade wax gourd polysaccharide solution and 0.03-0.05 parts by weight of potassium sorbate and continue stirring for 4-5min, the result is recorded as the mixed solution component; S3. Pour the glycerin component in S1 and the mixed liquid component in S2 into an emulsifying homogenizer for emulsification and homogenization. Use citric acid or triethanolamine to adjust the pH value to 6.0-6.5, and the resulting essence water is hydrating and antioxidant.

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