Low-irritation soothing skin repair compositions and methods for making the same

Abstract

The application discloses a low-irritation soothing skin repair composition and a preparation method thereof. The preparation method of the low-irritation soothing skin repair composition comprises the following steps: step (1), 5-15 parts of shaddac fruit and 2-15 parts of black elderberry are washed with water for 3-5 times, then hot air drying is carried out at 50-70 DEG C for 16-28h, crushing, and sieving through a 50-70 mesh sieve to obtain raw material powder; step (2), preparing a raw material extract; step (3), the raw material extract, 5-20 parts of collagen polypeptide, 2-9 parts of Prunus humilis oil, 1-5 parts of black elderberry fermentation, 5-15 parts of coffee silver skin extract, 2-10 parts of glycerol and 100 parts of deionized water are stirred and mixed to obtain the low-irritation soothing skin repair composition. The low-irritation soothing skin repair composition prepared by the application has good soothing, moisturizing and antioxidant effects.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a low-irritation soothing skin repair composition and its preparation method. Background Technology

[0002] Due to increasingly severe environmental pollution, the development of diverse cosmetics, and people's growing awareness of skin health, concerns about sensitive skin are gradually increasing. Sensitive skin (SS), also known as sensitive skin syndrome, is characterized by high reactivity and poor tolerance, making it prone to allergies. It is a skin condition in which the skin barrier function is impaired, mainly occurring on the face. Subjective symptoms include burning, redness, stinging, and itching, which may be accompanied by objective signs such as erythema, telangiectasia, and desquamation. In severe cases, allergic reactions such as swelling and rashes may occur.

[0003] Patent CN 114209633 B discloses an anti-inflammatory, soothing, and repairing composition, its preparation method, and its application. It is composed of cactus extract, oat bran extract, ophiopogon root extract, peony root extract, scutellaria root extract, corydalis root extract, elderberry flower extract, mallow flower extract, butylene glycol, pentylene glycol, and water. It has anti-inflammatory and soothing effects, but its effects are relatively singular, and its repairing and moisturizing effects are insufficient. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a low-irritation soothing skin repair composition and its preparation method that has good skin repair effect, soothes the skin, has no side effects, and has moisturizing and antioxidant effects.

[0005] Therefore, the technical solution provided in this application is as follows:

[0006] The first aspect of the present invention provides a low-irritation, soothing skin repair composition.

[0007] According to the present invention, the low-irritation soothing skin repair composition comprises the following components in parts by weight:

[0008] 5-15 parts grapefruit peel, 5-20 parts collagen peptides, 2-9 parts sea buckthorn fruit oil, 2-15 parts black elderberry fruit, 1-5 parts black elderberry ferment, 5-15 parts coffee bark extract, 2-10 parts glycerin, 100 parts deionized water.

[0009] Preferably, the collagen polypeptide is composed of palmitoyl tripeptide, palmitoyl pentapeptide, and acetyl hexapeptide-8.

[0010] More preferably, the mass ratio of palmitoyl tripeptide, palmitoyl pentapeptide, and acetyl hexapeptide-8 is 1-4:2-5:2-11.

[0011] Preferably, the method for preparing the black elderberry ferment includes the following steps:

[0012] Add 1.5-3 kg of crushed black elderberry fruit to 25-50 L of water and stir at 15-25℃ for 12-36 h. Filter the mixture and add 2.5-5 kg ​​of sucrose, 0.5-1 kg of SCOBY, and 2.5-5 L of kombucha starter to the filtrate. Ferment at 20-24℃ for 7-21 days. Filter the mixture through sterile gauze and evaporate it to dryness to obtain the fermented black elderberry product.

[0013] Preferably, the preparation method of coffee bark extract includes the following steps:

[0014] After grinding the coffee silver bark, the mixture is passed through a 40-60 mesh sieve and subjected to supercritical CO2 extraction for 1-2 hours at an extraction pressure of 250-300 bar, an extraction temperature of 40-60℃, and a liquid CO2 flow rate of 8-12 L / min to obtain a mixture. The mixture is then placed in a separator and separated at a pressure of 5-8 MPa and a temperature of 60-65℃ to obtain gaseous CO2 and the extract. The extract is dissolved in shea butter to obtain a 20-40 wt% coffee silver bark extract.

[0015] The second aspect of the present invention provides a method for preparing the low-irritation soothing skin repair composition described in the first aspect of the present invention.

[0016] According to the present invention, the method for preparing the low-irritation soothing skin repair composition includes the following steps:

[0017] Step (1) Wash 5-15 parts of grapefruit peel and 2-15 parts of black elderberry fruit with water 3-5 times, dry them with hot air at 50-70℃ for 16-28 hours, pulverize them, and pass them through a 50-70 mesh sieve to obtain raw material powder.

[0018] Step (2) Mix the raw material powder with deionized water at a material-to-liquid ratio of 1:30-1:50 (m / m), and extract with ultrasonic power of 200-300W at 50-70℃ for 40-60 minutes. Then, centrifuge at 7000-9000r / min for 5-15 minutes to obtain the raw material extract.

[0019] Step (3) Mix the raw material extract, 5-20 parts collagen peptides, 2-9 parts sea buckthorn fruit oil, 1-5 parts black elderberry ferment, 5-15 parts coffee bark extract, 2-10 parts glycerin, and 100 parts deionized water to obtain a low-irritation soothing skin repair composition.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The coffee bark extract of this invention is rich in bioactive components with antioxidant, antibacterial, antifungal and prebiotic properties, which help to form a keratinized lipid membrane to maintain the homeostasis of the permeability barrier. It also interacts with the components of sea buckthorn fruit oil, black elderberry extract and fermentation products, grapefruit peel extract and collagen peptides to provide good moisturizing, antioxidant and soothing repair effects. Moreover, most of them are plant extracts and the ingredients are safe and non-irritating.

[0022] 2. The coffee husks in this invention contain bioactive molecules such as phenols, flavonoids, phytosterols, and alkaloids. The lipolysis and hydrolysis of diacylglycerols and triacylglycerols therein produce fatty acids that participate in the synthesis of acylceramides, which help form a keratinized lipid membrane to maintain the permeability barrier. The alkaloid caffeine protects the skin from aging caused by oxidative stress by activating autophagy, counteracts the effects of ultraviolet radiation, slows down photoaging, and increases the microcirculation of blood in the skin. It also has skin compatibility and safety. Moreover, the coffee husks are the largest by-product of the roasting process, making it green and environmentally friendly.

[0023] 3. The sea buckthorn fruit oil in this invention contains a large amount of unsaturated fatty acids and fat-soluble vitamins, which can increase the physiological lipid content of the skin and accelerate the repair of damaged sebum film. Sea buckthorn fruit oil has the effects of clearing heat and reducing inflammation, detoxifying, and inhibiting bacteria. Black elderberry extract and fermentation products contain high levels of various phenolic compounds, which can effectively prevent cell damage caused by free radicals, stimulate the metabolism and proliferation of fibroblasts and keratinocytes, and have antioxidant and antibacterial effects. They work synergistically with sea buckthorn fruit oil to improve the stability of sea buckthorn fruit oil. The polyphenol components of the fermentation products are more diversified and have an inhibitory effect on elastase and collagenase, thus playing a role in delaying aging. Collagen peptides have good moisturizing and water-locking properties, and the natural zwitterionic structure also endows collagen peptides with good water solubility, emulsification, and buffering effects. They work synergistically with other raw materials to play the role of emulsifying colloids, stabilizing foam, and regulating pH value. Detailed Implementation

[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0025] Example 1

[0026] This embodiment discloses a method for preparing black elderberry ferment, including the following steps:

[0027] Add 2.1 kg of crushed black elderberry fruit to 32 L of water, stir at 20 °C for 24 h, filter, add 3.8 kg of sucrose, 0.75 kg of SCOBY and 3.8 L of kombucha starter to the filtrate, ferment at 22 °C for 14 days, filter through sterile gauze, evaporate to dryness to obtain black elderberry fermented product.

[0028] Example 2

[0029] This embodiment discloses a method for preparing coffee bark extract, including the following steps:

[0030] After grinding the coffee bark, the mixture was passed through a 50-mesh sieve and subjected to supercritical CO2 extraction for 1.5 hours at an extraction pressure of 275 bar, an extraction temperature of 50 °C, and a liquid CO2 flow rate of 10 L / min. The mixture was then placed in a separator and separated at a pressure of 7 MPa and a temperature of 62 °C to obtain gaseous CO2 and the extract. The extract was dissolved in shea butter to obtain a 30 wt% coffee bark extract.

[0031] Example 3

[0032] This embodiment discloses a method for preparing a low-irritation, soothing skin repair composition, comprising the following steps:

[0033] Step (1) Wash 1 kg of grapefruit peel and 0.8 kg of black elderberry fruit with water 4 times, dry them with hot air at 60℃ for 22 hours, pulverize them, and pass them through a 60-mesh sieve to obtain raw material powder;

[0034] Step (2) Mix the raw material powder with deionized water at a ratio of 1:40 (m / m) and extract with ultrasonic power at 250W at 60℃ for 50 min. Then, centrifuge at 8000r / min for 10 min to obtain the raw material extract.

[0035] Step (3) Mix the raw material extract, 1.4 kg collagen peptides, 0.5 kg sea buckthorn oil, 0.3 kg black elderberry ferment, 1 kg coffee bark extract, 0.6 kg glycerin and 10 kg deionized water to obtain a low-irritation soothing skin repair composition.

[0036] Example 4

[0037] This embodiment discloses a method for preparing a low-irritation, soothing skin repair composition, comprising the following steps:

[0038] Step (1) Wash 0.5kg of grapefruit peel and 1.5kg of black elderberry fruit with water 5 times, dry them with hot air at 50℃ for 28h, pulverize them, and pass them through a 50-mesh sieve to obtain raw material powder.

[0039] Step (2) Mix the raw material powder with deionized water at a ratio of 1:30 (m / m) and extract with ultrasonic at 70°C for 40 min at a power of 200W. Then centrifuge at 9000 r / min for 5 min to obtain the raw material extract.

[0040] Step (3) Mix the raw material extract, 2kg collagen peptides, 0.9kg sea buckthorn oil, 0.1kg black elderberry ferment, 1.5kg coffee bark extract, 0.2kg glycerin and 10kg deionized water to obtain a low-irritation soothing skin repair composition.

[0041] Example 5

[0042] This embodiment discloses a method for preparing a low-irritation, soothing skin repair composition, comprising the following steps:

[0043] Step (1) Wash 1.5kg of grapefruit peel and 0.2kg of black elderberry fruit with water three times, dry them with hot air at 70℃ for 16h, pulverize them, and pass them through a 70-mesh sieve to obtain raw material powder;

[0044] Step (2) Mix the raw material powder with deionized water at a ratio of 1:50 (m / m) and extract with ultrasonic at 50°C for 60 min using 200W power. Then, centrifuge at 7000r / min for 15 min to obtain the raw material extract.

[0045] Step (3) Mix the raw material extract, 0.5 kg collagen peptides, 0.2 kg sea buckthorn oil, 0.5 kg black elderberry ferment, 0.5 kg coffee bark extract, 1 kg glycerin, and 10 kg deionized water to obtain a low-irritation soothing skin repair composition.

[0046] Comparative Example 1

[0047] Compared with Example 3, Comparative Example 1 did not add grapefruit peel during the preparation of the low-irritation soothing skin repair composition, and all other conditions remained unchanged.

[0048] Comparative Example 2

[0049] Compared with Example 3, Comparative Example 2 did not add black elderberry fruit during the preparation of the low-irritation soothing skin repair composition, while all other conditions remained unchanged.

[0050] Comparative Example 3

[0051] Compared with Example 3, Comparative Example 3 did not add black elderberry ferment during the preparation of the low-irritation soothing skin repair composition, and all other conditions remained unchanged.

[0052] Comparative Example 4

[0053] Compared with Example 3, Comparative Example 4 did not add coffee bark extract during the preparation of the low-irritation soothing skin repair composition, and all other conditions remained unchanged.

[0054] Comparative Example 5

[0055] Compared with Example 3, Comparative Example 5 did not add sea buckthorn oil during the preparation of the low-irritation soothing skin repair composition, and all other conditions remained unchanged.

[0056] Comparative Example 6

[0057] Compared with Example 3, Comparative Example 6 did not add collagen peptides during the preparation of the low-irritation soothing skin repair composition, while all other conditions remained unchanged.

[0058] Effect test

[0059] I. Test for reduction in skin moisture loss

[0060] Eighteen subjects aged 20-40 with dry skin were selected. A 3×3cm mark was made on the inner side of the anterior wall of both hands of the subjects. 2 The test area can be marked with multiple areas on the same arm, spaced 1 cm apart. Test samples and blank controls are randomly distributed on both arms. A capacitance-based skin assay instrument is used to measure the test and control areas. Each area is measured 15 times in parallel. First, the blank paper for each test area is measured, then the sample is measured at 2.0 ± 0.1 mg / cm². 2 The product was evenly applied to the test area. Skin moisture content in the test area and the control area was measured at 1, 2, and 4 hours after application (measurements were performed at these times during validation). No products (cosmetics or topical medications) should be used on the test site for 2-3 days prior to the test. The test environment temperature was controlled at 25±1℃, and the relative humidity at 40±5%. Before the test, the subjects' arms were wiped with purified water at approximately 36℃, and the subjects were allowed to sit quietly in the test environment for 30 minutes before the test. Transepidermal water loss (TEWL) was measured at each time point according to the experimental design, and the reduction in skin moisture loss at each time point was calculated. The greater the reduction in skin moisture loss, the better the skin barrier repair effect. The average reduction in skin moisture loss was taken, and the results are shown in Table 1.

[0061] Calculation formula: Reduction in skin water loss = [(T0-T1) / T0]×100%, where T1 is the TEWL of the test group at each time point, and T0 is the TEWL of the blank control group at each time point.

[0062] Table 1

[0063]

[0064] As shown in Table 1, after being applied to the skin, Examples 3-5 all reduced the skin's moisture loss within a certain period of time. Among them, Example 3 showed the most significant reduction effect. At 30 minutes, the skin's moisture loss was reduced by 32.57%, and at 120 minutes, the reduction was 29.56%, indicating that Example 3 has a good effect on repairing the skin barrier and can effectively improve the dryness of the skin. In comparison with Comparative Examples 1-6, it can be clearly seen that if any one of the following is missing: grapefruit peel, black elderberry fruit, black elderberry ferment, coffee bark extract, sea buckthorn oil, or collagen peptides, the skin repair effect of the composition is greatly reduced.

[0065] II. Test on the effect of soothing sensitive stimuli

[0066] Eighteen volunteers with sensitive skin, aged 20-35 years, were selected, with an equal number of men and women. Volunteers were prohibited from using any soothing products, such as cosmetics, topical medications, or oral supplements, for 30 days prior to the experiment. Before the experiment, volunteers sat quietly for 30 minutes in a temperature- and humidity-controlled room at 22°C and 50% humidity. During the experiment, half of the face was tested using a randomized table; one side was used for Examples 3-5, and the other side for Comparative Examples 1-6. The nasolabial fold area was selected for the test. 50 μL of 10% lactic acid solution was dropped onto a single-layer filter paper with a diameter of 0.8 cm. The participants were asked about their stinging sensation at 3, 4, and 5 minutes, and scored using a 4-point scale (0 for no stinging, 1 for mild stinging, 2 for moderate stinging, and 3 for severe stinging; scores could be taken from adjacent stinging intervals based on the actual situation). The volunteers' scores were recorded as the lactic acid stimulation score (D0) for that side. After the test, volunteers were required to use the samples as instructed: one side of the face using Examples 3-5, and the other side using Comparative Examples 1-6. Cross-contamination was to be avoided during use, and the samples were to be used once daily as instructed. Follow-up visits were required on day 14 (D14) and day 28 (D28) of the trial. Lighting conditions should be consistent at each time point: curtains closed, adequate lighting. Subjects lay on a bed. The angle of the bed tilt should remain consistent at each time point. The bed's position must be marked on the ground to ensure consistency between D14 and D28. Testing of the same volunteer was performed by the same measurement personnel. Scores were recorded and rates of change were calculated.

[0067] The calculation method is as follows: Test result change rate = (DO - Dd) / DOX × 100%, where DO is the lactate stimulation score before sample use, and Dd is the lactate stimulation score measured at each follow-up time. The average value of the test result change rate is taken.

[0068] The test results are shown in Table 2.

[0069] Table 2

[0070]

[0071] Table 2 clearly shows the difference between the lactic acid irritation scores of Examples 3-5 and Comparative Examples 1-6. Since grapefruit peel, black elderberry fruit, black elderberry ferment, coffee bark extract, sea buckthorn oil, and collagen peptides are used in combination, the soothing effect is significantly reduced if any one of these substances is missing. Comparison of the D14 and D28 test results shows that long-term use of Examples 3-5 can enhance the skin's resistance to irritation. Through the comparison of Examples 3-5, it can be seen that Example 3 has the best soothing effect.

[0072] III. Barrier Repair and Swelling Reduction Effect Test

[0073] Ninety individuals aged 20-40 with minor skin lesions were selected and divided into 9 groups of 10 each. The subjects' symptoms included dryness, peeling, itching, and erythema, with no significant differences between groups. Subjects used the sample once daily for 28 consecutive days. Random assessments were conducted before and after 28 days of use. The indicators were graded as follows: 0 = worsening, 1 = slight aggravation, 2 = no significant change, 3 = slight improvement, 4 = significant improvement, 5 = no obvious symptoms. Test results are averages and rounded to the nearest whole number. The test results are shown in Table 3.

[0074] Table 3

[0075] Group dry Peeling Itching erythema Example 3 4 4 4 4 Example 4 4 4 3 4 Example 5 3 4 4 4 Comparative Example 1 3 3 3 2 Comparative Example 2 3 3 2 3 Comparative Example 3 2 3 2 3 Comparative Example 4 2 1 1 1 Comparative Example 5 2 2 2 2 Comparative Example 6 1 1 1 2

[0076] As shown in Table 3, the low-irritation soothing skin repair composition provided by this invention has varying degrees of soothing effect on the damaged skin in individuals with minor skin damage. Example 3 showed the best effect, significantly improving dryness, peeling, itching, and erythema. This improvement was also more pronounced compared to Comparative Examples 1-6, indicating that the absence of any one of the following ingredients in the composition—grapefruit peel, black elderberry fruit, black elderberry ferment, coffee bark extract, sea buckthorn oil, or collagen peptides—would significantly reduce the soothing effect of the composition.

[0077] IV. Antioxidant Performance Test

[0078] (1) Evaluation of superoxide anion free radical scavenging ability

[0079] Take 4.5 mL of 0.05 mol / L Tris-HCl buffer solution (pH = 8.2) and preheat it in a 25°C water bath for 30 min. Then add 1 mL of the compositions from Examples 3-5 and Comparative Examples 1-6 and 0.4 mL of 25 mol / L pyrogallol solution, mix well, and react in a 25°C water bath for 5 min. Stop the reaction by adding 1.0 mL of 8 mol / L HCl. Use the Tris-HCl buffer solution as a reference and measure the absorbance at 299 nm. Replace the sample with 1 mL of the sample solvent for the blank control. The superoxide anion radical scavenging rate (%) = [1 - (A2 / A1)] × 100%, where A1 is the absorbance value of the blank control; A2 is the absorbance value of the corresponding sample.

[0080] (2) Evaluation of scavenging ability against hydroxyl radicals

[0081] Add 3 mL of 2 mmol / L FeSO4 and 3 mL of 1 mmol / L H2O2 sequentially to a 25 mL cuvette, shake well, then add 3 mL of 6 mmol / L salicylic acid, shake well, heat in a 37°C water bath for 15 min, and measure the absorbance. Add the compositions of Examples 3-5 and Comparative Examples 1-6 respectively, shake well, continue heating in a water bath for 15 min, and measure the absorbance. The hydroxyl radical scavenging rate (%) is calculated as [A0 - Ax - (A0 - Ax0)] / A0 × 100%, where A0 is the absorbance value of the reaction system before adding the sample; Ax is the absorbance value of the system after the sample scavenges hydroxyl radicals; and Ax0 is the absorbance value of the system after the blank control scavenges hydroxyl radicals. The test results are shown in Table 4.

[0082] Table 4

[0083] Group Superoxide anion radical scavenging rate / % Hydroxyl radical scavenging rate / % Example 3 98.21 97.12 Example 4 96.96 96.37 Example 5 95.21 95.07 Comparative Example 1 85.64 84.69 Comparative Example 2 80.21 80.56 Comparative Example 3 79.86 78.54 Comparative Example 4 79.51 79.22 Comparative Example 5 84.67 83.21 Comparative Example 6 90.52 91.23

[0084] As shown in Table 4, the low-irritation soothing skin repair compositions provided in Examples 3-4 exhibit a superoxide anion free radical scavenging rate of over 95% and a hydroxyl free radical scavenging rate of over 95%. This fully demonstrates that the components of the low-irritation soothing skin repair compositions of the present invention work synergistically to effectively scavenge free radicals and restore the skin's antioxidant protective capacity, with significant improvements. Comparative Examples 1-6 show that the absence of any one of the following ingredients in the added compositions—grapefruit peel, black elderberry fruit, black elderberry ferment, coffee bark extract, sea buckthorn oil, or collagen peptides—significantly reduces the antioxidant effect of the compositions.

[0085] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

[0086] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to specific implementations. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A low-irritation, soothing skin repair composition, characterized in that, The raw materials of the repair composition are composed of the following components in parts by weight: 5-15 parts grapefruit peel, 5-20 parts collagen polypeptide, 2-9 parts prickly pear oil, 2-15 parts black elderberry fruit, 1-5 parts black elderberry ferment, 5-15 parts coffee bark extract, 2-10 parts glycerin, and 100 parts deionized water. The collagen polypeptide is composed of palmitoyl tripeptide, palmitoyl pentapeptide, and acetyl hexapeptide-8; The mass ratio of palmitoyl tripeptide, palmitoyl pentapeptide, and acetyl hexapeptide-8 is 1-4:2-5:2-11; The preparation process of the repair composition includes the following steps: Step (1) Wash 5-15 parts of grapefruit peel and 2-15 parts of black elderberry fruit with water 3-5 times, dry them with hot air at 50-70℃ for 16-28 hours, pulverize them, and pass them through a 50-70 mesh sieve to obtain raw material powder. Step (2) Mix the raw material powder with deionized water at a ratio of 1:30-1:50 and extract with ultrasonic power at 200-300W at 50-70℃ for 40-60 minutes. Then centrifuge at 7000-9000r / min for 5-15 minutes to obtain the raw material extract. Step (3) Mix the raw material extract, 5-20 parts of collagen peptides, 2-9 parts of sea buckthorn fruit oil, 1-5 parts of black elderberry ferment, 5-15 parts of coffee bark extract, 2-10 parts of glycerin, and 100 parts of deionized water to obtain a low-irritation soothing skin repair composition. The preparation method of the black elderberry ferment includes the following steps: Add 1.5-3 kg of crushed black elderberry fruit to 25-50 L of water and stir at 15-25℃ for 12-36 h. Filter the mixture and add 2.5-5 kg ​​of sucrose, 0.5-1 kg of SCOBY, and 2.5-5 L of kombucha starter to the filtrate. Ferment at 20-24℃ for 7-21 days. Filter the mixture through sterile gauze and evaporate it to dryness to obtain the black elderberry fermented product. The preparation method of coffee bark extract includes the following steps: After grinding the coffee silver bark, the mixture is passed through a 40-60 mesh sieve and subjected to supercritical CO2 extraction for 1-2 hours at an extraction pressure of 250-300 bar, an extraction temperature of 40-60℃, and a liquid CO2 flow rate of 8-12 L / min to obtain a mixture. The mixture is then placed in a separator and separated at a pressure of 5-8 MPa and a temperature of 60-65℃ to obtain gaseous CO2 and the extract. The extract is dissolved in shea butter to obtain a 20-40 wt% coffee silver bark extract.

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