A hyaluronic acid functional dressing for promoting wound healing and a method for preparing the same

Abstract

The application provides a hyaluronic acid functional dressing for promoting wound healing and a preparation method thereof, the hyaluronic acid functional dressing comprising a dressing component and a non-woven fabric, the dressing component comprising the following raw materials in parts by weight: 30-45 parts of polysaccharide macromolecular polymerized sodium hyaluronate, 22-28 parts of yeast-beta-glucan, 14-18 parts of glucomannan, 16-25 parts of gallic acid, 26-32 parts of palmitoyl peptide, 8-13 parts of 1,2-pentanediol and 10-15 parts of soybean protein isolate. In the hyaluronic acid functional dressing, the mutual synergistic effect of various raw materials in the dressing component can effectively inhibit bacteria, promote skin cell growth, promote wound healing and repair the skin, and the like. The hyaluronic acid functional dressing can be used for promoting wound healing and skin repair and the like, has a wide application range and has a wide application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of dressings, in particular to a hyaluronic acid functional dressing for promoting wound healing and a preparation method thereof. BACKGROUND

[0002] Skin is an important protective barrier of the human body, which can not only prevent the invasion of exogenous substances, but also effectively prevent the loss of electrolytes, water and nutrients. However, due to endogenous and exogenous injuries, such as cuts, puncture wounds, and burns, wounds can be formed on the surface of the skin, thereby damaging the structure and normal function of the skin tissue, and causing problems such as exudate, bleeding, tissue necrosis or infection. Acne is also a common follicular sebaceous gland disease in dermatology, which is manifested as skin damage such as comedones, pustules or cysts, and has also affected the physical and mental health and quality of life of patients. After the skin is damaged, healing needs a process, in order to improve the healing time of skin damage and alleviate the pain of patients, a dressing is often needed to cover the wound to provide a good healing environment for the wound, thereby accelerating the healing time. At present, various dressings appear on the market, such as hemostatic dressings, bacteriostatic dressings, anti-inflammatory dressings, etc., and the emphases of various dressings are not the same for different skin problems. Hyaluronic acid is an anionic mucopolysaccharide, which is the main component of connective tissue such as human body intercellular matrix, joint synovial fluid and eye vitreous body, and plays important physiological functions such as water retention, lubrication, osmotic pressure regulation, maintenance of extracellular space and promotion of cell repair in the body. At present, although some hyaluronic acid functional dressings appear on the market, the component ratio of each component is unreasonable, and an effective wound healing environment cannot be formed, the wound healing effect is slow, and at present there is no hyaluronic acid dressing for promoting wound healing that can be used for acne, skin repair and other skin problems. SUMMARY

[0003] In view of this, the present application provides a hyaluronic acid functional dressing for promoting wound healing and a preparation method thereof.

[0004] The technical scheme of the present application is as follows:

[0005] A hyaluronic acid functional dressing for promoting wound healing, comprising a dressing component and a non-woven fabric, the dressing component comprising the following raw materials in parts by weight: 30-45 parts of polysaccharide macromolecular polyhyaluronic acid sodium, 22-28 parts of yeast-beta-glucan, 14-18 parts of glucomannan, 16-25 parts of gallic acid, 26-32 parts of palmitoyl peptide, 8-13 parts of 1,2-pentanediol, and 10-15 parts of soybean protein isolate.

[0006] Furthermore, the dressing components include the following raw materials in parts by weight: 35 parts of polysaccharide macromolecular polymerized sodium hyaluronate, 26 parts of yeast-β-glucan, 15 parts of glucomannan, 22 parts of gallic acid, 30 parts of palmitoyl peptide, 9 parts of 1,2-pentanediol, and 12 parts of soy protein isolate.

[0007] Furthermore, the preparation method of the polysaccharide macromolecular polymerized sodium hyaluronate is as follows: after stirring and mixing sodium hyaluronate and purified water, spirulina polysaccharide and polyvinyl pyrrolidone are added and stirred and mixed evenly, thereby obtaining the polysaccharide macromolecular polymerized sodium hyaluronate.

[0008] Furthermore, the mass ratio of the sodium hyaluronate, spirulina polysaccharide, polyvinyl pyrrolidone and purified water is 5-8:0.5-2.0:0.1-0.3:15-20; the stirring and mixing temperature is 30-40° C., and the stirring time is 15-30 min.

[0009] Furthermore, the palmitoyl peptide includes palmitoyl dipeptide-7, palmitoyl tripeptide-5 and palmitoyl pentapeptide-4; the mass ratio of palmitoyl dipeptide-7, palmitoyl tripeptide-5 and palmitoyl pentapeptide-4 is 1:5~7:3~4.

[0010] A method for preparing a hyaluronic acid functional dressing for promoting wound healing comprises the following steps:

[0011] Step (1): adding polysaccharide macromolecular polymerized sodium hyaluronate to purified water and stirring, and then adding yeast-β-glucan and palmitoyl peptide and stirring and mixing evenly to form phase A;

[0012] Step (2): Mix glucomannan and glycerol aqueous solution uniformly, then add soy protein isolate, gallic acid and 1,2-pentanediol and stir to mix uniformly, as phase B;

[0013] Step (3): Mix phase A and phase B evenly to obtain a dressing component, and then evenly apply the dressing component on the surface of the non-woven fabric to obtain the target hyaluronic acid functional dressing.

[0014] Furthermore, in step (1), the mass ratio of the polysaccharide macromolecular polymerized sodium hyaluronate to purified water is 1:2-4; and in step (2), the mass ratio of the glucomannan to the glycerol aqueous solution is 1:3-5.

[0015] Furthermore, in step (2), the mass concentration of the glycerol aqueous solution is 15-20%.

[0016] Furthermore, in step (3), the temperature of mixing phase A and phase B is 30-35°C.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. The hyaluronic acid functional dressing of the present invention, through the synergistic effect of the various raw materials in the dressing components, can achieve effective antibacterial effects, promote skin cell growth, thereby promoting wound healing, and repairing the skin. The interaction between the polysaccharide macromolecular polymer sodium hyaluronate, glucomannan, and soy protein isolate in the hyaluronic acid functional dressing can create a good repair environment for the wound surface. Under the synergistic effect of yeast-β-glucan, palmitoyl peptide, and 1,2-pentanediol, the cell repair function is enhanced and skin cell growth is promoted. After compounding, the gallic acid has excellent antibacterial and anti-inflammatory effects, promoting rapid wound healing.

[0019] 2. The present invention mixes spirulina polysaccharide with sodium hyaluronate to prepare cross-linked polysaccharide macromolecular polymer sodium hyaluronate, which can improve the stability and moisturizing properties of sodium hyaluronate, provide a good healing environment for the wound, thereby promoting the proliferation and differentiation of epidermal cells, promoting skin regeneration at the injured site, and accelerating healing efficiency.

[0020] 3. The hyaluronic acid functional dressing of the present invention can be used to treat acne, promote wound healing and skin repair, etc. It has a wide range of applications and broad application prospects. DETAILED DESCRIPTION

[0021] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0022] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0023] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0024] Example 1

[0025] The hyaluronic acid functional dressing of this embodiment for promoting wound healing includes a dressing component and a non-woven fabric. The dressing component includes the following raw materials in parts by weight: 30 parts of polysaccharide macromolecular polymerized sodium hyaluronate, 22 parts of yeast-β-glucan, 14 parts of glucomannan, 18 parts of gallic acid, 28 parts of palmitoyl peptide composed of palmitoyl dipeptide-7, palmitoyl tripeptide-5 and palmitoyl pentapeptide-4 in a mass ratio of 1:5:3, 12 parts of 1,2-pentanediol, and 14 parts of soy protein isolate;

[0026] The preparation method of the polysaccharide macromolecular polymerized sodium hyaluronate is as follows: sodium hyaluronate and purified water are stirred and mixed at 40℃ for 15 min, and then spirulina polysaccharide and polyvinylpyrrolidone are added and stirred and mixed at 35℃ for 20 min to be uniform, to obtain the polysaccharide macromolecular polymerized sodium hyaluronate; wherein the mass ratio of sodium hyaluronate, spirulina polysaccharide, polyvinylpyrrolidone and purified water is 5:1.5:0.1:16.

[0027] Example 2

[0028] The hyaluronic acid functional dressing for promoting wound healing in this example comprises a dressing component and a non-woven fabric, and the dressing component comprises the following raw materials in parts by weight: 35 parts of polysaccharide macromolecular polymerized sodium hyaluronate, 26 parts of yeast-β-glucan, 15 parts of glucomannan, 22 parts of gallic acid, 30 parts of palmitoyl peptide composed of palmitoyl dipeptide-7, palmitoyl tripeptide-5 and palmitoyl pentapeptide-4 in a mass ratio of 1:5:4, 9 parts of 1,2-pentanediol and 12 parts of soybean protein isolate.

[0029] Example 3

[0030] The hyaluronic acid functional dressing for promoting wound healing in this example comprises a dressing component and a non-woven fabric, and the dressing component comprises the following raw materials in parts by weight: 45 parts of polysaccharide macromolecular polymerized sodium hyaluronate, 28 parts of yeast-β-glucan, 14 parts of glucomannan, 25 parts of gallic acid, 26 parts of palmitoyl peptide composed of palmitoyl dipeptide-7, palmitoyl tripeptide-5 and palmitoyl pentapeptide-4 in a mass ratio of 1:7:4, 10 parts of 1,2-pentanediol and 15 parts of soybean protein isolate.

[0031] The preparation method of the hyaluronic acid functional dressing for promoting wound healing in examples 1-3 comprises the following steps:

[0032] Step (1): according to the mass ratio of 1:3, polysaccharide macromolecular polymerized sodium hyaluronate is added to purified water and stirred and mixed uniformly, and then yeast-β-glucan and palmitoyl peptide are added and stirred and mixed uniformly, as phase A;

[0033] Step (2): according to the mass ratio of 1:5, glucomannan is added to a 16% glycerol aqueous solution and mixed uniformly, and then soybean protein isolate, gallic acid and 1,2-pentanediol are added and stirred and mixed uniformly, as phase B;

[0034] Step (3): phase A and phase B are mixed uniformly at 30℃ to obtain the dressing component, and then the dressing component is evenly applied on the surface of the non-woven fabric to obtain the target hyaluronic acid functional dressing.

[0035] Comparative Example 1

[0036] Compared with Example 3, in the present comparative example, the polysaccharide macromolecular polymer sodium hyaluronate is replaced by sodium hyaluronate, and the rest is the same as Example 3.

[0037] Comparative Example 2

[0038] Compared with Example 3, the present comparative example does not contain the components of yeast-β-glucan and palmitoyl peptide, and the rest is the same as Example 3.

[0039] The hyaluronic acid functional dressing for promoting wound healing in the present example comprises a dressing component and a non-woven fabric, and the dressing component comprises the following raw materials in parts by weight: 45 parts of polysaccharide macromolecular polymer sodium hyaluronate, 14 parts of glucomannan, 25 parts of gallic acid, 10 parts of 1,2-pentanediol, and 15 parts of soybean protein isolate.

[0040] The preparation method of the hyaluronic acid functional dressing for promoting wound healing in Examples 1-3 comprises the following steps:

[0041] Step (1): The polysaccharide macromolecular polymer sodium hyaluronate is added to purified water in a mass ratio of 1:3 and stirred to mix uniformly, as phase A;

[0042] Step (2): The glucomannan is added to a glycerol aqueous solution with a mass concentration of 16% in a mass ratio of 1:5, and then the soybean protein isolate, gallic acid and 1,2-pentanediol are added and stirred to mix uniformly, as phase B;

[0043] Step (3): The phases A and B are mixed uniformly at 30°C to obtain the dressing component, and then the dressing component is evenly applied to the surface of the non-woven fabric to obtain the target hyaluronic acid functional dressing.

[0044] Comparative Example 3

[0045] Compared with Example 3, the preparation method of the present comparative example is different. The specific process is as follows:

[0046] The preparation method comprises the following steps: the polysaccharide macromolecular polymer sodium hyaluronate is added to purified water in a mass ratio of 1:8 and stirred to mix uniformly, and then 3 times the mass of the polysaccharide macromolecular polymer sodium hyaluronate is added to a glycerol aqueous solution with a mass concentration of 16%, yeast-β-glucan, palmitoyl peptide, glucomannan, soybean protein isolate, gallic acid and 1,2-pentanediol are mixed uniformly at 30°C to obtain the dressing component, and then the dressing component is evenly applied to the surface of the non-woven fabric to obtain the target hyaluronic acid functional dressing.

[0047] The target hyaluronic acid functional dressings prepared in Examples 1-3 and Comparative Examples 1-3 of the present application are used for antibacterial and wound repair tests.

[0048] (1) Bacteriostatic test

[0049] ①Strain sample: Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes and Monilia albican were selected as the strain sample.

[0050] ②Preparation of test bacterial solution: under sterile conditions, the sterilized culture medium was filled into test tubes and formed into slopes, then the strain sample was inoculated by streaking method, and then placed in an incubator, the bacteria were cultured at 37°C for 24h, and the fungi were cultured at 28°C for 3-5 days; under sterile conditions, 2 rings of the cultured strain sample were picked up with an inoculating loop, diluted with normal saline, and mixed uniformly to prepare a test bacterial solution with a concentration of 10 5 cfu / mL.

[0051] ③Preparation of test sample: the hyaluronic acid functional dressing prepared in Examples 1-3 and Comparative Examples 1-3 was prepared into a test sample with a concentration of 0.05g / mL by adding sterile water to the dressing components.

[0052] ③Test process: Examples 1-3 and Comparative Examples 1-3 were used as each group, and 5 plate cultures were set for each group; under sterile conditions, 100μL of the test bacterial solution was evenly coated on the plate culture, 6 sterile Oxford cups were placed equidistantly on each culture, then 0.2ml of the test sample was added to each Oxford cup, then the culture medium containing Staphylococcus aureus, Escherichia coli and Streptococcus pyogenes was cultured in an incubator at a temperature of 37°C for 24h, and the culture medium containing Monilia albican was cultured in an incubator at a temperature of 28°C for 3-5 days. At the same time, the culture medium added with 100μL of sterile water was used as a blank control group for the same culture test. After the culture was completed, the Oxford cups were removed, then the diameter L of each bacteriostatic ring was accurately measured with a vernier caliper, each culture was tested 3 times, and the average value was taken as the result, and the bacteriostatic effect was compared.

[0053] Bacteriostatic rate (%) = (L0-L1) / L0x100%

[0054] (Note: L0 is the bacteriostatic ring diameter of the blank control group, and L1 is the bacteriostatic ring diameter of Examples 1-3 and Comparative Examples 1-3, respectively)

[0055] (2) Wound healing test

[0056] Select 80 rabbits of similar age and weight, anesthetize the rabbits with anesthetic, then depilate the same position on the back of the rabbit to form a test area of 3x3cm with depilatory cream, then use a sterile scalpel to draw a "#" shape wound in the test area, finally, make the wound purulent, red and swollen to indicate the success of the modeling; randomly select 56 rabbits with successful modeling, and randomly divide them into 7 groups, 10 rabbits in each group, and apply the hyaluronic acid functional dressing prepared by Examples 1-3 and Comparative Examples 1-3 to the wound, respectively, and record the healing of the wound. Replace the dressing every day for 4 days, and then do not use the dressing for 4 days to allow the wound to heal naturally, and observe the conditions of the wound, such as suppuration, scabbing, scabbing, and redness. The number of effective cases can be determined when the wound appears scabbing, no bleeding, no pus, or scabbing falls off, and the number of effective cases is recorded.

[0057] Table 1 Hyaluronic acid functional dressing test situation:

[0058]

[0059] The above results show that the hyaluronic acid functional dressing prepared by Examples 1-3 of the present application has good antibacterial properties, and under the synergistic effect of each component such as polysaccharide macromolecular polymerized sodium hyaluronate, yeast-β-glucan, palmitoyl peptide and gallic acid, it can have better antibacterial effect, can inhibit bacteria such as Staphylococcus aureus, Streptococcus pyogenes and Candida albicans, has good antibacterial effect, can provide a good healing environment for acne, wounds and the like, reduce the invasion of bacteria, and thus improve the healing effect. The dressing prepared in Comparative Example 1 does not contain polysaccharide macromolecular polymerized sodium hyaluronate, resulting in poor stability of the dressing and a poor healing environment, thus making the antibacterial and wound healing promoting efficiency poor. In Comparative Example 2, yeast-β-glucan and palmitoyl peptide are not contained, resulting in slow wound repair and also reducing the antibacterial effect. The preparation method of Comparative Example 3 is different from that of Example 3, indicating that the multiple cross-linked sodium hyaluronate dressing can be obtained by the preparation method of the present application, the components of the dressing can be uniformly distributed, and good synergistic effect can be achieved.

[0060] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A hyaluronic acid functional dressing for promoting wound healing, comprising a dressing component and a non-woven fabric, characterized in that: The dressing component comprises the following raw materials in parts by weight: 30-45 parts of polysaccharide macromolecular polymerized sodium hyaluronate, 22-28 parts of yeast-β-glucan, 14-18 parts of glucomannan, 16-25 parts of gallic acid, 26-32 parts of palmitoyl peptide, 8-13 parts of 1,2-pentanediol, and 10-15 parts of soy protein isolate; The polysaccharide macromolecular polymerized sodium hyaluronate comprises sodium hyaluronate, spirulina polysaccharide, polyvinyl pyrrolidone and purified water in a mass ratio of 5-8:0.5-2.0:0.1-0.3:15-20; The hyaluronic acid functional dressing for promoting wound healing is prepared by the following steps: Step (1): adding polysaccharide macromolecular polymerized sodium hyaluronate to purified water and stirring, and then adding yeast-β-glucan and palmitoyl peptide and stirring and mixing evenly to form phase A; Step (2): Mix glucomannan and glycerol aqueous solution evenly, then add soy protein isolate, gallic acid and 1,2-pentanediol and stir to mix evenly, as phase B; Step (3): Mix phase A and phase B evenly to obtain a dressing component, and then evenly apply the dressing component on the surface of the non-woven fabric to obtain the target hyaluronic acid functional dressing.

2. The hyaluronic acid functional dressing for promoting wound healing according to claim 1, characterized in that: The dressing components include the following raw materials in parts by weight: 35 parts of polysaccharide macromolecular polymerized sodium hyaluronate, 26 parts of yeast-β-glucan, 15 parts of glucomannan, 22 parts of gallic acid, 30 parts of palmitoyl peptide, 9 parts of 1,2-pentanediol, and 12 parts of soy protein isolate.

3. The hyaluronic acid functional dressing for promoting wound healing according to any one of claims 1 or 2, characterized in that: The preparation method of the polysaccharide macromolecular polymerized sodium hyaluronate is as follows: sodium hyaluronate and purified water are stirred and mixed, and then spirulina polysaccharide and polyvinyl pyrrolidone are added and stirred and mixed evenly to obtain the polysaccharide macromolecular polymerized sodium hyaluronate.

4. The hyaluronic acid functional dressing for promoting wound healing according to claim 3, characterized in that: The stirring and mixing temperature is 30-40° C., and the stirring time is 15-30 min.

5. The hyaluronic acid functional dressing for promoting wound healing according to claim 1, characterized in that: The palmitoyl peptide includes palmitoyl dipeptide-7, palmitoyl tripeptide-5 and palmitoyl pentapeptide-4; the mass ratio of palmitoyl dipeptide-7, palmitoyl tripeptide-5 and palmitoyl pentapeptide-4 is 1:5~7:3~4.

6. The hyaluronic acid functional dressing for promoting wound healing according to claim 1, characterized in that: In step (1), the mass ratio of the polysaccharide macromolecular polymerized sodium hyaluronate to purified water is 1:2-4; in step (2), the mass ratio of the glucomannan to the glycerol aqueous solution is 1:3-5.

7. The hyaluronic acid functional dressing for promoting wound healing according to claim 1, characterized in that: In step (2), the mass concentration of the glycerol aqueous solution is 15-20%.

8. The hyaluronic acid functional dressing for promoting wound healing according to claim 1, characterized in that: In step (3), the temperature of mixing phase A and phase B is 30-35°C.

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