An in-situ patterned peroxide-releasing antibacterial fiber membrane and a preparation method thereof

Abstract

The application discloses an antibacterial fiber membrane releasing peroxide in situ and a preparation method thereof, and relates to the technical field of fiber membrane preparation.The fiber membrane comprises a fiber membrane core layer and a fiber membrane shell layer coated outside the fiber membrane core layer, and metal peroxide nanoparticles are dispersed in the fiber membrane shell layer; the fiber membrane core layer is a non-water-soluble high polymer, and the fiber membrane shell layer is a water-soluble high polymer and a non-water-soluble high polymer.The application provides a large number of adhesion sites by combining the fiber structure and instant patterning at a wound site, promotes cell adhesion and guides cell migration, and metal peroxide nanoparticles released along with the patterning process react with moisture in the internal environment to release hydrogen peroxide, oxygen and metal ions, thereby achieving the effects of sterilization and promoting blood vessel regeneration simultaneously.The application solves the problem that the existing skin repair materials cannot achieve sterilization and cell growth promotion.

Description

Technical Field

[0001] The present invention relates to the technical field of fiber membrane preparation, and in particular to an antibacterial fiber membrane capable of in-situ patterned peroxide release and a preparation method thereof. Background Art

[0002] As the first barrier to protect the human body, the skin separates the internal environment of the human body from the external environment, maintains the stability of the internal environment, and blocks the invasion of microorganisms in the external environment. However, the skin is easily affected by daily physical activities of the human body, causing mechanical trauma, burns, frostbite, etc. The defective parts are susceptible to bacterial infection due to the lack of barrier function, resulting in various complex and serious physiological changes, which in turn pose a threat to human life. For some serious deep wounds, pressure sores, lower limb venous ulcer wounds, and chronic wounds of diabetic patients, the skin cannot completely heal on its own and must be treated with medical means. However, traditional skin repair materials cannot create a physical and chemical environment that is conducive to cell growth and wound healing, and it is difficult to prevent further invasion of external microorganisms. With the increase in obesity, diabetes and aging populations, the incidence of difficult-to-heal wounds has increased, which has put forward new requirements for new wound dressings that shorten healing time and fight bacterial infections. Summary of the Invention

[0003] In order to solve the above technical problems, the purpose of the present invention is to provide an antibacterial fiber membrane that releases peroxide in an in situ patterned manner and a preparation method thereof, so as to solve the problem that existing skin repair materials cannot achieve sterilization and promote cell growth.

[0004] The technical solution of the present invention to solve the above technical problems is as follows: an antibacterial fiber membrane for in-situ patterned peroxide release is provided, comprising a fiber membrane core layer and a fiber membrane shell layer coated on the outside of the fiber membrane core layer, wherein metal peroxide nanoparticles are dispersed in the fiber membrane shell layer; the fiber membrane core layer is a water-insoluble polymer, and the fiber membrane shell layer is a water-soluble polymer and a water-insoluble polymer.

[0005] On the basis of the above technical solution, the present invention can also be improved as follows:

[0006] Furthermore, the water-insoluble high molecular polymer is polycaprolactone, polylactic acid, polylactic acid-glycolic acid, polytetrafluoroethylene or polyvinylidene fluoride.

[0007] Furthermore, the water-soluble high molecular polymer is polyvinyl pyrrolidone, polyvinyl butyral, polyethylene oxide, polyvinyl alcohol, chitosan, collagen, gelatin or a gelatin derivative.

[0008] Furthermore, the metal peroxide is at least one of calcium peroxide, copper peroxide, magnesium peroxide, zinc peroxide, barium peroxide, cerium peroxide and silver peroxide.

[0009] Furthermore, the metal peroxide nanoparticles are prepared by the following method: mixing a metal salt and polyvinyl pyrrolidone, dissolving the mixture in deionized water, adding a sodium hydroxide solution and stirring, then adding hydrogen peroxide and stirring for 25-35 minutes, washing, and freeze-drying to prepare the metal peroxide nanoparticles.

[0010] Furthermore, the mass volume ratio of the metal salt, polyvinyl pyrrolidone, deionized water, sodium hydroxide solution and hydrogen peroxide is 0.05-0.1 g: 2 g: 45-55 mL: 4-6 mL: 1-3 mL.

[0011] Furthermore, the mass volume ratio of the metal salt, polyvinyl pyrrolidone, deionized water, sodium hydroxide solution and hydrogen peroxide is 0.08 g:2 g:50 mL:5 mL:2 mL.

[0012] Furthermore, the concentration of the sodium hydroxide solution is 0.08-0.12 mol / L.

[0013] Furthermore, the concentration of the sodium hydroxide solution is 0.1 mol / L.

[0014] Furthermore, the core layer of the fiber membrane is a water-insoluble high molecular polymer and a loading substance, and the loading substance is curcumin, IL-4 or VEGF.

[0015] The present invention also provides a method for preparing the above-mentioned antibacterial fiber membrane capable of in-situ patterned peroxide release, comprising the following steps:

[0016] (1) dissolving a water-insoluble polymer in an organic solvent and stirring the mixture to obtain a fiber membrane core layer solution;

[0017] (2) dissolving a water-soluble polymer and a water-insoluble polymer in an organic solvent and stirring the mixture to obtain a fiber membrane shell emulsion;

[0018] (3) adding metal peroxide nanoparticles to the fiber membrane shell emulsion prepared in step (2), dispersing the nanoparticles uniformly, and stirring for 10-15 hours to obtain an emulsion-suspension composite liquid;

[0019] (4) The fiber membrane core layer solution obtained in step (1) and the emulsion-suspension composite solution obtained in step (3) are subjected to electrospinning and vacuum drying to obtain an antibacterial fiber membrane that releases peroxide in situ patterning.

[0020] Furthermore, in steps (1)-(2), the organic solvent is at least one of trifluoroethanol, hexafluoroisopropanol, dichloromethane, chloroform, trifluoroacetic acid and N,N-dimethylformamide.

[0021] Furthermore, in steps (1)-(2), the organic solvent is trifluoroethanol.

[0022] Furthermore, in steps (1)-(2), stirring is performed for 6-24 hours.

[0023] Furthermore, in steps (1)-(2), stirring is performed for 8-12 hours.

[0024] Furthermore, in step (1), the concentration of the water-insoluble high molecular polymer in the fiber membrane core layer solution is 8-20 wt%.

[0025] Furthermore, in step (1), the concentration of the water-insoluble high molecular polymer in the fiber membrane core layer solution is 9-12 wt %.

[0026] Furthermore, in step (2), the mass ratio of the water-soluble polymer to the water-insoluble polymer is 1:0.9-1.2.

[0027] Furthermore, in step (2), the mass ratio of the water-soluble polymer to the water-insoluble polymer is 1:1.

[0028] Furthermore, in step (2), the concentration of the fiber membrane shell emulsion is 6-15 wt%.

[0029] Furthermore, in step (2), the concentration of the fiber membrane shell emulsion is 8-12 wt%.

[0030] Furthermore, in step (3), the concentration of the metal peroxide nanoparticles in the fiber membrane shell emulsion prepared in step (2) is 3-10 mg / mL.

[0031] Furthermore, in step (3), the concentration of the metal peroxide nanoparticles in the fiber membrane shell emulsion prepared in step (2) is 5-8 mg / mL.

[0032] Furthermore, in step (4), the fiber membrane core layer solution obtained in step (1) is placed in the core layer extrusion device of the electrospinning equipment, and the emulsion-suspension composite solution obtained in step (3) is placed in the shell layer extrusion device of the electrospinning equipment for electrospinning.

[0033] Furthermore, the electrospinning process parameters are: voltage 6-20 kV, injection speed 0.5-1.5 mL / h, and receiving distance 10-20 cm.

[0034] Furthermore, the electrospinning process parameters are: voltage 6-10 kV, injection speed 0.6-1.2 mL / h, and receiving distance 12-18 cm.

[0035] Furthermore, the electrospinning process parameters were: voltage 8 kV, injection speed 1 mL / h, and receiving distance 15 cm.

[0036] Furthermore, in step (4), the arrangement of the electrospun fiber membrane is random arrangement, oriented arrangement, centripetal arrangement, grid arrangement or honeycomb arrangement.

[0037] The present invention also provides the use of the above-mentioned in-situ patterned peroxide-releasing antibacterial fiber membrane in the preparation of wound repair materials.

[0038] The present invention has the following beneficial effects:

[0039] 1. The present invention adopts an electrospinning process to combine two immiscible polymers on the fiber surface. When the antibacterial fiber membrane contacts the wound, the soluble polymer components of the shell dissolve, and a nano-groove structure is patterned in situ on the fiber surface. At the same time, the metal peroxide nanoparticles in the fiber shell are quickly released. The nano-groove structure increases the roughness of the fiber surface, which is conducive to cell adhesion and proliferation along the fiber direction. The released metal peroxide nanoparticles can react with water in the wound environment to produce hydrogen peroxide and hydroxyl free radicals, giving the fiber membrane a rapid bactericidal function. At the same time, the metal ions exert a regulatory effect on cell metabolism, thereby achieving accelerated wound healing and angiogenesis.

[0040] 2. The present invention provides a large number of adhesion sites by combining fiber structures and their immediate patterning at the wound site, thereby enhancing cell adhesion and guiding cell migration, thereby promoting tissue regeneration; at the same time, the metal peroxide nanoparticles released during the patterning process react with moisture in the internal environment to release hydrogen peroxide and oxygen, and further convert hydrogen peroxide into hydroxyl free radicals to achieve rapid and efficient sterilization. At the same time, the released metal ions can effectively induce cell metabolism, enhance the angiogenic effect, and effectively achieve wound surface vascular regeneration.

[0041] 3. The electrospinning fiber membrane preparation method provided by the present invention can simply combine two immiscible polymers on the fiber surface, and use the soluble polymer to quickly dissolve under the action of internal environmental moisture to form a grooved nanostructure on the surface of a single fiber, effectively solving the problems of high cost, low efficiency, and complex steps of existing fiber membrane structural modification methods.

[0042] 4. The present invention uses polymer-based fiber membrane as the base material, which plays an important role in the physical and chemical properties, degradation properties, and biocompatibility of the entire material system. The corresponding polymer base material can be selected according to the specific application scenario; in the coaxial electrospinning process, the double polymer droplets of the shell fluid are elongated by electrostatic action and solidified into a fiber shell at the same time. After the fiber shell comes into contact with moisture on the wound surface, the water-soluble polymer quickly dissolves and forms an in-situ patterned nanostructure, which helps to enhance cell adhesion and guide cell proliferation; and by introducing metal peroxide nanoparticles into the fiber shell fluid, they are released while being patterned and react with moisture in the wound environment to produce oxygen, hydrogen peroxide, hydroxyl free radicals, etc. for rapid sterilization. At the same time, its metal ions can further regulate cell metabolism and promote angiogenesis, solving the need for wound repair materials to be antibacterial, promote wound healing, and promote angiogenesis.

[0043] 5. The fiber membrane for in situ patterned peroxide release provided by the present invention has a typical extracellular matrix-like structure. The preparation method is stable and repeatable, simple to operate, and easy to scale up. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 The morphology of the fiber membranes prepared in Example 1 and Comparative Example 1;

[0045] Figure 2 This is a detection diagram of hydrogen peroxide produced by the fiber membrane prepared in Example 1;

[0046] Figure 3 This is a detection diagram of hydroxyl radicals generated by the fiber membrane prepared in Example 1;

[0047] Figure 4 This is a test chart of the antibacterial performance of the fiber membrane prepared in Example 1;

[0048] Figure 5 This is a test image of the fiber membrane wound repair prepared in Example 1. DETAILED DESCRIPTION

[0049] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples are only used to explain the present invention and are not intended to limit the scope of the invention. In the embodiments, if specific conditions are not specified, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments is not specified, they are all conventional products that can be purchased commercially.

[0050] Example 1:

[0051] An antibacterial fiber membrane capable of in-situ patterned peroxide release, the preparation method of which comprises the following steps:

[0052] (1) Dissolve 1.2 g of water-insoluble polymer polycaprolactone in 8.8 g of organic solvent trifluoroethanol and stir continuously for 20 h using a magnetic stirring device to prepare a fiber membrane core layer solution (concentration of 12 wt%);

[0053] (2) 0.5 g of water-soluble polymer polyvinyl pyrrolidone and 0.5 g of water-insoluble polymer polycaprolactone were dissolved in 9 g of organic solvent trifluoroethanol, and stirred continuously for 20 h using a magnetic stirring device to prepare a fiber membrane shell emulsion (concentration of 10 wt%);

[0054] (3) 80 mg of copper chloride dihydrate and 2 g of polyvinyl pyrrolidone were weighed and dissolved in 50 mL of deionized water. 5 mL of 0.1 mol / L sodium hydroxide solution was added and stirred for 5 min. Then, 2 mL of hydrogen peroxide was added and stirred for 30 min. The mixture was then centrifuged and washed with ethanol and water several times and freeze-dried to obtain copper peroxide nanoparticles.

[0055] (4) Add 35 mg of the copper peroxide nanoparticles prepared in step (3) to 5 mL of the fiber membrane shell emulsion prepared in step (2), ultrasonically disperse for 30 min, and stir for 12 h to prepare an emulsion-suspension composite solution (concentration of 7 mg / mL);

[0056] (5) The fiber membrane core layer solution obtained in step (1) is placed in the core layer extrusion device of the electrospinning equipment, and the emulsion-suspension composite liquid obtained in step (3) is placed in the shell layer extrusion device of the electrospinning equipment, that is, they are respectively loaded into 5mL syringes and placed in the two push injection devices of the electrospinning machine, respectively connected to the inner layer and outer layer of the coaxial needle, using a roller receiver, a spinning voltage of 8kV, a push injection speed of 1mL / h and 1.2mL / h respectively, and a receiving distance of 15cm to perform electrospinning, and then placed in a vacuum drying oven for one week to obtain an in situ patterned antibacterial fiber membrane that releases peroxide.

[0057] Example 2:

[0058] An antibacterial fiber membrane capable of in-situ patterned peroxide release, the preparation method of which comprises the following steps:

[0059] (1) 0.8 g of water-insoluble polymer polylactic acid was dissolved in 9.2 g of organic solvent hexafluoroisopropanol and stirred continuously for 6 h using a magnetic stirring device to prepare a fiber membrane core layer solution (concentration of 8 wt%);

[0060] (2) 0.5 g of water-soluble polymer gelatin and 0.5 g of water-insoluble polymer polylactic acid were dissolved in 15.67 g of organic solvent hexafluoroisopropanol, and stirred continuously for 6 h using a magnetic stirring device to prepare a fiber membrane shell emulsion (concentration of 6 wt%);

[0061] (3) 80 mg of copper chloride dihydrate and 2 g of polyvinyl pyrrolidone were weighed and dissolved in 50 mL of deionized water. 5 mL of 0.1 mol / L sodium hydroxide solution was added and stirred for 5 min. Then, 2 mL of hydrogen peroxide was added and stirred for 30 min. The mixture was then centrifuged and washed with ethanol and water several times and freeze-dried to obtain copper peroxide nanoparticles.

[0062] (4) adding 15 mg of the copper peroxide nanoparticles prepared in step (3) to 5 mL of the fiber membrane shell emulsion prepared in step (2), ultrasonically dispersing for 30 min, and stirring for 10 h to obtain an emulsion-suspension composite solution (concentration of 3 mg / mL);

[0063] (5) The fiber membrane core layer solution obtained in step (1) is placed in the core layer extrusion device of the electrospinning equipment, and the emulsion-suspension composite liquid obtained in step (3) is placed in the shell layer extrusion device of the electrospinning equipment, that is, they are respectively loaded into 5mL syringes and placed in the two push injection devices of the electrospinning machine, respectively connected to the inner layer and outer layer of the coaxial needle, using a roller receiver, a spinning voltage of 6kV, push injection speeds of 0.5mL / h and 0.5mL / h respectively, and a receiving distance of 10cm, for electrospinning, and then placed in a vacuum drying oven for drying for one week to obtain an in situ patterned antibacterial fiber membrane that releases peroxide.

[0064] Example 3:

[0065] An antibacterial fiber membrane capable of in-situ patterned peroxide release, the preparation method of which comprises the following steps:

[0066] (1) Dissolve 2 g of water-insoluble polymer polylactic acid-glycolic acid in 8 g of organic solvent trifluoroethanol and stir continuously for 24 h using a magnetic stirring device to prepare a fiber membrane core layer solution (concentration of 20 wt%);

[0067] (2) 1 g of water-soluble polymer polyethylene oxide and 1 g of water-insoluble polymer polylactic acid-glycolic acid were dissolved in 11.33 g of organic solvent dichloromethane, and stirred continuously for 24 h using a magnetic stirring device to prepare a fiber membrane shell emulsion (concentration of 15 wt%);

[0068] (3) 80 mg of copper chloride dihydrate and 2 g of polyvinyl pyrrolidone were weighed and dissolved in 50 mL of deionized water. 5 mL of 0.1 mol / L sodium hydroxide solution was added and stirred for 5 min. Then, 2 mL of hydrogen peroxide was added and stirred for 30 min. The mixture was then centrifuged and washed with ethanol and water several times and freeze-dried to obtain copper peroxide nanoparticles.

[0069] (4) Add 50 mg of the copper peroxide nanoparticles prepared in step (3) to 5 mL of the fiber membrane shell emulsion prepared in step (2), ultrasonically disperse for 30 min, and stir for 15 h to obtain an emulsion-suspension composite solution (concentration of 10 mg / mL);

[0070] (5) The fiber membrane core layer solution obtained in step (1) is placed in the core layer extrusion device of the electrospinning equipment, and the emulsion-suspension composite liquid obtained in step (3) is placed in the shell layer extrusion device of the electrospinning equipment, that is, they are respectively loaded into 5mL syringes and placed in the two push injection devices of the electrospinning machine, respectively connected to the inner layer and outer layer of the coaxial needle, using a roller receiver, a spinning voltage of 20kV, push injection speeds of 1.5mL / h and 1.5mL / h respectively, and a receiving distance of 20cm, for electrospinning, and then placed in a vacuum drying oven for drying for one week to obtain an in situ patterned antibacterial fiber membrane that releases peroxide.

[0071] Comparative Example 1:

[0072] A method for preparing a polycaprolactone fiber membrane, wherein the raw materials include polycaprolactone and trifluoroethanol from Aladdin Company, and the specific steps are as follows:

[0073] (1) 1.5 g of polycaprolactone was dispersed in 8.5 g of trifluoroethanol and stirred for 12 h using a magnetic stirring device to obtain a spinning solution A with a concentration of 15 wt%;

[0074] (2) The spinning solution A obtained in step (1) was loaded into a 5 mL syringe and placed in the injection device of the electrospinning machine to prepare a polycaprolactone fiber membrane by electrospinning. A roller receiver was used, the spinning voltage was 10 kV, the injection speed was 1 mL / h, and the receiving distance was 15 cm;

[0075] (3) The fiber membrane obtained in step (2) is placed in a vacuum drying oven and dried for one week to obtain a polycaprolactone fiber membrane.

[0076] Test example

[0077] 1. Morphology and patterning performance

[0078] The antibacterial fiber membrane prepared in Example 1 and the polycaprolactone fiber membrane prepared in Comparative Example 1 were subjected to morphology and pattern detection. The specific detection method was as follows: a fiber membrane with a diameter of 1 cm was placed in a 50 mL centrifuge tube; 40 mL of phosphate buffer was added to each, and the membrane was placed in a constant temperature shaking table and fully immersed at 37°C for 24 hours; the soaked fiber membrane was taken out, rinsed with deionized water 2-3 times, and then placed in a freeze dryer to fully dry for 24 hours; the dried sample was sprayed with gold, and the fiber morphology was observed by scanning electron microscopy. The results show that Figure 1 ( Figure 1 The left side is before soaking, and the right side is after soaking).

[0079] Depend on Figure 1 It can be seen that before immersion, the fibers of Example 1 and Comparative Example 1 maintained a smooth and flat fiber structure, while after immersion, a groove-like structure arranged along the fiber direction appeared on the surface of the single fiber of Example 1, indicating that the fiber membrane prepared by the present invention has patterning capabilities.

[0080] 2. Hydrogen Peroxide and Hydroxyl Radical Generation Capacity

[0081] The antibacterial fiber membrane prepared in Example 1 was cultured with titanium sulfate solution (1 mg / mL) and tetramethylbenzidine (40 μg / mL) in carbonate buffer, respectively. The hydrogen peroxide and hydroxyl radical generation abilities of the antibacterial fiber membrane were determined by the color change of the culture medium.

[0082] The specific steps are as follows: immerse a 1 cm diameter antibacterial fiber membrane disc in 2 mL of phosphate buffer. After incubation for different time periods, remove the fiber membrane and add 1 mL of the incubated phosphate buffer to 1 mL of titanium sulfate solution, shake it evenly, and use a photometer to detect the absorbance of the solution at 412 nm. Statistical values ​​of absorbance at different time periods are shown in the table. Figure 2 ;

[0083] The antibacterial fiber membrane disc with a diameter of 1 cm was immersed in 2 mL of carbonate buffer containing tetramethylbenzidine. After incubation for different time periods, the fiber membrane was taken out and the absorption spectrum of the buffer at wavelengths of 450-800 nm after the fiber membrane was immersed for different time periods was measured by UV-visible spectroscopy. The results are shown in Figure 2. Figure 3 .

[0084] Depend on Figure 2-3 It can be seen that the antibacterial fiber membrane prepared in the present invention can release peroxide in situ, thereby reacting with water to produce hydrogen peroxide and hydroxyl radicals.

[0085] 3. Antibacterial properties

[0086] The antibacterial fiber membrane prepared in Example 1 was tested for its antibacterial properties. The specific testing method was as follows: a circular fiber membrane with a diameter of 1 cm was placed in a 24-well cell culture plate; Staphylococcus aureus and Escherichia coli were inoculated onto the fiber membrane at a density of 106 CFU / mL, respectively, and cultured for 3 hours; the culture solution was discarded, and the fiber membrane was gently rinsed with physiological saline, and the fiber membrane was immersed in 40wt% paraformaldehyde and fixed for 30 minutes; gradient dehydration was performed with an ethanol-water mixed solution; the bacterial morphology on the fiber membrane was observed by scanning electron microscopy after gold spraying, and the results were shown in FIG. Figure 4 ( Figure 4 In the figure, Escherichia coli is on the left and Staphylococcus aureus is on the right).

[0087] Depend on Figure 4It can be seen that the bacterial structures of Escherichia coli and Staphylococcus aureus were destroyed, indicating that the production of hydrogen peroxide and hydroxyl radicals combined with metal ions gave the fiber membrane good antibacterial properties.

[0088] 4. Wound Repair

[0089] The antibacterial fiber membrane and gauze prepared in Example 1 and the fiber membrane prepared in Comparative Example 1 were respectively covered in the diabetic rat skin defect model, and the wound repair effect was observed on the 0th, 4th and 14th days. Figure 5 .

[0090] Depend on Figure 5 It can be seen that the wound covered by ordinary gauze repairs slowly and it is difficult to achieve wound healing within 14 days; the healing speed of comparative example 1 is improved compared with ordinary gauze, but skin defects still exist after 14 days; Example 1 shows an obvious effect of promoting wound healing within 14 days, and the wound is almost completely closed after 14 days, indicating that the in situ patterned peroxide-releasing fiber membrane prepared by the present invention has good ability to guide tissue regeneration and repair, and has broad application prospects in the field of wound repair.

[0091] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An antibacterial fiber membrane with in-situ patterned peroxide release, characterized in that: The fiber membrane comprises a fiber membrane core layer and a fiber membrane shell layer coated on the outside of the fiber membrane core layer, wherein metal peroxide nanoparticles are dispersed in the fiber membrane shell layer; the fiber membrane core layer is a water-insoluble polymer, and the fiber membrane shell layer is a water-soluble polymer and a water-insoluble polymer; The water-insoluble high molecular polymer is polycaprolactone, polylactic acid, polylactic acid-glycolic acid, polytetrafluoroethylene or polyvinylidene fluoride; The water-soluble high molecular polymer is polyvinyl pyrrolidone, polyvinyl butyral, polyethylene oxide, polyvinyl alcohol or chitosan; The metal peroxide is at least one of calcium peroxide, copper peroxide, magnesium peroxide, zinc peroxide, barium peroxide and cerium peroxide.

2. The method for preparing an antibacterial fiber membrane capable of in-situ patterned peroxide release according to claim 1, characterized in that: The following steps are involved: (1) Dissolving a water-insoluble polymer in an organic solvent and stirring evenly to obtain a fiber membrane core layer solution; (2) dissolving a water-soluble polymer and a water-insoluble polymer in an organic solvent and stirring them uniformly to prepare a fiber membrane shell emulsion; (3) adding metal peroxide nanoparticles to the fiber membrane shell emulsion obtained in step (2), dispersing the particles uniformly, and stirring for 10-15 hours to obtain an emulsion-suspension composite liquid; (4) The fiber membrane core layer solution prepared in step (1) and the emulsion-suspension composite solution prepared in step (3) are subjected to electrospinning and vacuum drying to obtain an antibacterial fiber membrane that releases peroxide in situ patterning.

3. The method for preparing an antibacterial fiber membrane capable of in-situ patterned peroxide release according to claim 2, wherein: In steps (1)-(2), the organic solvents are trifluoroethanol, hexafluoroisopropanol, dichloromethane, chloroform, trifluoroacetic acid and N,N -At least one of dimethylformamide.

4. The method for preparing an antibacterial fiber membrane capable of in-situ patterned peroxide release according to claim 2, wherein: In step (1), the concentration of the water-insoluble high molecular polymer in the fiber membrane core layer solution is 8-20 wt%.

5. The method for preparing an antibacterial fiber membrane capable of in-situ patterned peroxide release according to claim 2, wherein: In step (2), the mass ratio of the water-soluble polymer to the water-insoluble polymer is 1:0.9-1.

2.

6. The method for preparing an antibacterial fiber membrane capable of in-situ patterned peroxide release according to claim 2, wherein: In step (3), the concentration of the metal peroxide nanoparticles in the fiber membrane shell emulsion prepared in step (2) is 3-10 mg / mL.

7. Use of the in-situ patterned peroxide-releasing antibacterial fiber membrane according to claim 1 in the preparation of wound repair materials.

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