Application of a Chinese medicine composition in preparing anti-influenza virus medicine

Abstract

The present invention relates to the field of traditional Chinese medicine, and specifically provides an application of a traditional Chinese medicine composition in the preparation of an anti-influenza virus drug. The traditional Chinese medicine composition is obtained by repeatedly optimizing the composition and content of the drugs in the compound through clinical research and basic research. In the prescription, Artemisia annua and Scutellaria baicalensis are combined as the main drugs; Gypsum, Folium Isatidis, Flos Lonicerae, and Indigo Naturalis are collectively used as the ministerial drugs; Poria, Tangerine Peel, Pinellia, Rhizoma Anemarrhenae, Periostracum Cicadae, and Mentha are collectively used as the adjuvant drugs; and Licorice is the guiding drug of the prescription. The above-mentioned drugs have the effects of clearing the lungs and relieving heat, harmonizing the stomach and removing dampness, have strong antipyretic and fever-reducing, laxative and expectorant effects, and play a good inhibitory effect on influenza virus infection.

Description

Technical Field

[0001] The present invention relates to the field of traditional Chinese medicine, and in particular to application of a traditional Chinese medicine composition in preparing medicines against influenza viruses. Background Art

[0002] Influenza virus is also called influenza virus for short, and it is divided into influenza A, B, C and D viruses. Influenza virus can cause infection and disease in many animals such as humans, poultry, pigs, etc. The typical clinical symptoms of these diseases are fever, body aches, fatigue, and respiratory symptoms such as sneezing, runny nose, sore throat, and shortness of breath. Human influenza is mainly caused by influenza A virus and influenza B virus, both of which are contagious. Among them, influenza A (H1N1) is a highly contagious acute respiratory disease with a high incidence rate and rapid transmission, which can be transmitted through direct or indirect contact.

[0003] At present, common drugs for treating influenza virus in clinic include oseltamivir, zanamivir and other neuraminidase inhibitors, which are favored by people because of their good antiviral effect, but these drugs may cause adverse reactions such as insomnia, nausea, vomiting, dizziness, bronchitis, etc. Traditional Chinese medicine has the characteristics of small side effects and holistic treatment. Therefore, it is still necessary to develop new Chinese medicine with good therapeutic effect and small toxic side effects. Summary of the invention

[0004] Therefore, the object of the present invention is to provide a use of a Chinese medicine composition in the preparation of anti-influenza virus drugs.

[0005] The Chinese medicine composition includes the following raw materials in parts by weight, 100-300 parts of Artemisia annua, 100-300 parts of Scutellaria baicalensis, 100-450 parts of Poria cocos, 100-270 parts of Tangerine peel, 100-270 parts of Rhizoma Anemarrhenae, 580-820 parts of Gypsum, 200-450 parts of Honeysuckle, 200-450 parts of Folium Isatidis, 100-300 parts of Pinellia ternata, 100-270 parts of Periostracum Cicadae, 100-270 parts of Mint, 100-270 parts of Licorice and 20-180 parts of Indigo Naturalis.

[0006] Furthermore, in parts by weight, the Chinese medicine composition includes the following raw materials: 200-300 parts of Artemisia annua, 200-300 parts of Scutellaria baicalensis, 250-450 parts of Poria cocos, 150-270 parts of dried tangerine peel, 150-270 parts of Rhizoma Anemarrhenae, 700-820 parts of Gypsum, 300-450 parts of Honeysuckle, 300-450 parts of Folium Isatidis, 200-300 parts of Pinellia ternata, 150-270 parts of Periostracum Cicadae, 150-270 parts of Mint, 150-270 parts of Licorice and 50-150 parts of Indigo Naturalis.

[0007] Furthermore, in parts by weight, the Chinese medicine composition includes the following raw materials: 100-150 parts of Artemisia annua, 100-150 parts of Scutellaria baicalensis, 150-200 parts of Poria cocos, 100-150 parts of Tangerine peel, 100-150 parts of Rhizoma Anemarrhenae, 580-700 parts of Gypsum, 200-300 parts of Honeysuckle, 200-300 parts of Folium Isatidis, 150-200 parts of Pinellia ternata, 100-150 parts of Periostracum Cicadae, 100-150 parts of Mint, 100-150 parts of Licorice and 40-50 parts of Indigo Naturalis.

[0008] Further, the Chinese medicine composition includes the following raw materials in parts by weight: 250 parts of Artemisia annua, 250 parts of Scutellaria baicalensis, 375 parts of Poria cocos, 150 parts of Tangerine peel, 150 parts of Rhizoma Anemarrhenae, 750 parts of Gypsum, 375 parts of Honeysuckle, 375 parts of Folium Isatidis, 250 parts of Pinellia ternata, 150 parts of Periostracum Cicadae, 150 parts of Menthus fragrans, 150 parts of Licorice and 50 parts of Indigo Naturalis; or,

[0009] The Chinese medicine composition comprises the following raw materials: 100 parts of Artemisia annua, 100 parts of Scutellaria baicalensis, 150 parts of Poria cocos, 100 parts of Tangerine peel, 100 parts of Rhizoma Anemarrhenae, 580 parts of Gypsum, 200 parts of Honeysuckle, 200 parts of Folium Isatidis, 150 parts of Pinellia ternata, 100 parts of Periostracum Cicadae, 100 parts of Mint, 100 parts of Licorice and 20 parts of Indigo Naturalis; or,

[0010] The Chinese medicine composition comprises the following raw materials: 200 parts of Artemisia annua, 250 parts of Scutellaria baicalensis, 170 parts of Poria cocos, 170 parts of Tangerine peel, 170 parts of Rhizoma Anemarrhenae, 650 parts of Gypsum, 350 parts of Honeysuckle, 250 parts of Folium Isatidis, 200 parts of Pinellia ternata, 170 parts of Periostracum Cicadae, 170 parts of Mentha, 170 parts of Licorice and 120 parts of Indigo Naturalis; or,

[0011] The Chinese medicine composition includes the following raw materials: 300 parts of Artemisia annua, 300 parts of Scutellaria baicalensis, 450 parts of Poria cocos, 270 parts of dried tangerine peel, 270 parts of Rhizoma Anemarrhenae, 820 parts of Gypsum, 450 parts of Honeysuckle, 450 parts of Folium Isatidis, 300 parts of Pinellia ternata, 270 parts of Periostracum Cicadae, 270 parts of Menthus fragrans, 170 parts of Licorice and 180 parts of Indigo Naturalis.

[0012] Furthermore, the raw materials of the traditional Chinese medicine composition do not contain Jiao San Xian.

[0013] Furthermore, the preparation method of the traditional Chinese medicine composition includes, the preparation method of the traditional Chinese medicine composition includes, mixing Artemisia annua, Scutellaria baicalensis, Poria cocos, tangerine peel, bamboo shavings, gypsum, honeysuckle, isatis indigotica, Pinellia ternata, Periostracum cicada, mint and licorice, extracting according to a conventional extraction method or extracting them separately according to a conventional extraction method and then mixing them, and mixing them into Indigo naturalis to obtain the composition; or, mixing Artemisia annua, Scutellaria baicalensis, Poria cocos, tangerine peel, bamboo shavings, gypsum, honeysuckle, isatis indigotica, Pinellia ternata, Periostracum cicada, mint, licorice and Indigo naturalis, extracting according to a conventional extraction method or extracting them separately according to a conventional extraction method and then mixing them to obtain the composition.

[0014] Furthermore, the conventional extraction method includes one or more of decoction extraction, maceration extraction, percolation extraction, reflux extraction, ultrasonic extraction and steam distillation extraction; the extraction solvent is selected from water or an alcohol solution with a volume percentage of 5-98%; the number of extractions is at least 1; the extraction time is at least 10 minutes; the ratio of the mass of the extraction solvent to the weight of the raw material is ≥2.

[0015] In certain preferred embodiments, the extraction solvent is selected from water or an alcohol solution with a volume percentage of 5-98%; the alcohol solution is an aqueous solution containing alcohol, and the alcohol can be ethanol or methanol.

[0016] In a specific embodiment, the extraction times are at least 1 time, preferably 1-5 times, and more preferably 2 times.

[0017] In a specific embodiment, the extraction time is at least 10 min, preferably 60-120 min, and more preferably 120 min.

[0018] In a specific embodiment, the ratio of the mass of the extraction solvent to the weight of the raw material drug is ≥2; preferably 6-15, and more preferably 10.

[0019] In certain preferred embodiments, conventional purification methods may be used for purification, such as but not limited to column chromatography, membrane filtration and the like.

[0020] The Chinese medicine composition is added with or without a pharmaceutically acceptable carrier and prepared into a pharmaceutical preparation according to a conventional preparation method.

[0021] In certain preferred embodiments, the pharmaceutical preparation is selected from a liquid preparation, a solid preparation or a semisolid preparation.

[0022] In a specific embodiment, the pharmaceutical preparation is a gel, cream, tablet, capsule, powder, mixture, pill, granule, syrup or decoction.

[0023] In a specific embodiment, the pharmaceutically acceptable excipient is selected from at least one of a pharmaceutically acceptable solvent, a solubilizer, a cosolvent, an emulsifier, a colorant, a binder, a disintegrant, a filler, a lubricant, a wetting agent, a glidant, a flavoring agent, a preservative, a suspending agent, a coating material, an anti-adhesive, a penetration enhancer, a pH adjuster and a thickener.

[0024] Furthermore, the auxiliary material includes a filler. The mass ratio of the dry drug powder obtained by mixing with Indigo Naturalis after drying to the filler is 8:2-7:3. The moisture content of the material after drying is 5%-6.5%.

[0025] Furthermore, the ratio of the mass of the finished drug preparation to the mass of the raw drug used is 1000:2000-5000, preferably 1000:3425, wherein the mass of the raw drug is the sum of the masses of all the raw drugs.

[0026] The present invention also provides a method for preparing the pharmaceutical preparation, comprising the following steps:

[0027] The preparation method of the pharmaceutical preparation comprises the following steps:

[0028] Decoction step: taking gypsum and adding extraction solvent to decoct, collecting the decoction liquid and the residue to obtain A; taking Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Honeysuckle, Folium Isatidis, Pinellia ternata, Periostracum Cicadae, Mentha, and Licorice to mix, adding A, adding extraction solvent to soak, decoct and extract, separate the solid and liquid, collect the liquid, and obtain the liquid;

[0029] The preparation steps of the pharmaceutical preparation are: concentrating and / or drying the medicinal solution, mixing it with Indigo Naturalis, adding or not adding auxiliary materials, and preparing the pharmaceutical preparation according to conventional processes; or,

[0030] Decoction step: taking gypsum and adding extraction solvent to decoct, collecting the decoction liquid and the residue to obtain A; taking Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Honeysuckle, Folium Isatidis, Pinellia ternata, Periostracum Cicadae, Mentha, Licorice, Indigo naturalis, mixing, adding A, adding extraction solvent to soak, decoct and extract, separate solid and liquid, collect liquid, and obtain liquid;

[0031] The preparation steps of the pharmaceutical preparation are: concentrating and / or drying the drug solution, adding or not adding auxiliary materials, and preparing the pharmaceutical preparation according to conventional processes.

[0032] In certain preferred embodiments, the drying step further comprises a pulverizing step.

[0033] In certain preferred embodiments, the solid-liquid separation is selected from filtration or centrifugation.

[0034] In a more preferred embodiment, the decoction step comprises: taking gypsum, adding 3-6 times the weight of gypsum water or 5-98% alcohol solution and decocting for 30-90 minutes, collecting the decoction and the residue to obtain A; taking Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Honeysuckle, Folium Isatidis, Pinellia ternata, Periostracum Cicadae, Mentha, and Licorice, mixing, adding A, adding water to 5-12 times the weight of the medicinal materials in water or 5-98% alcohol solution, soaking for 30-120 minutes, decocting and extracting 1-4 times, the material-liquid mass ratio of each extraction is 1:6-15, the extraction time is 60-120 minutes, solid-liquid separation, collecting the liquid, and obtaining a medicinal solution.

[0035] Among them, Artemisia annua and Scutellaria baicalensis are used as the main medicines in the prescription. Artemisia annua is bitter, cold and aromatic, and can clear away the evil heat in Shaoyang. "New Edition of Materia Medica" says that it "clears away heat and fire without consuming qi and blood". Scutellaria baicalensis is bitter and cold, is good at clearing heat from the gallbladder, and can dry out dampness. The combination of the two medicines can clear away damp heat in Shaoyang and expel evil.

[0036] The prescription uses gypsum, isatis indigotica, honeysuckle and indigo as assistant medicines. Gypsum is pungent and cold in nature, can reduce heat and clear the fire of the lungs and stomach; isatis indigotica is bitter and cold in nature, can clear away heat and detoxify, cool blood and eliminate freckles; honeysuckle is sweet and cold in nature, is especially good at clearing heat and detoxifying, and dispersing wind-heat; indigo is salty and cold in nature, can clear away heat and detoxify, purge fire and calm the nerves.

[0037] In addition, Poria cocos, tangerine peel, Pinellia, bamboo shavings, cicada molt and mint are used as adjuvants. Among them, Poria cocos and tangerine peel strengthen the spleen and regulate qi, Pinellia and bamboo shavings resolve phlegm and stop vomiting, and cicada molt and mint disperse wind-heat.

[0038] Finally, licorice is used as a guiding drug to clear away heat, detoxify and harmonize various medicines.

[0039] The present invention also provides an antiviral serum, which is serum collected after an animal or a human orally takes any of the above-mentioned traditional Chinese medicine compositions or pharmaceutical preparations.

[0040] Furthermore, serum is collected at least 0.5 hours after oral administration; and / or, the animal is a rat, a mouse or a guinea pig; and / or, the oral dose of the Chinese medicine composition or pharmaceutical preparation is 10-50 g / kg; and / or, the collection site is the femoral artery.

[0041] Furthermore, before oral administration, the Chinese medicine composition or pharmaceutical preparation is added with an aqueous solution to prepare a liquid with a concentration of 0.1-1.0 g / mL.

[0042] Furthermore, the aqueous solution is water, glucose aqueous solution or physiological saline water.

[0043] The present invention also provides the use of the antiviral serum in preparing drugs for resisting influenza viruses.

[0044] Furthermore, the influenza virus is influenza A virus; the influenza virus is influenza A virus; preferably, the influenza A virus includes one or more of H1N1 and H3N2 viruses; more preferably, the H1N1 virus includes H1N1 wild type or H1N1 influenza virus oseltamivir-resistant strain.

[0045] The technical solution of the present invention has the following advantages:

[0046] 1. The Chinese medicine composition provided by the present invention is obtained after repeatedly optimizing the composition and content of the drugs in the compound through clinical research and basic research. In the prescription, Artemisia annua and Scutellaria baicalensis are combined as the main drugs; Gypsum, Folium Isatidis, Flos Lonicerae, and Indigo Naturalis are collectively used as the ministerial drugs; Poria, Tangerine Peel, Pinellia, Rhizoma Anemarrhenae, Periostracum Cicadae, and Mentha are collectively used as the adjuvant drugs; and Licorice is the guiding drug of the prescription. The above-mentioned drugs together play the effects of clearing the lungs and relieving heat, harmonizing the stomach and removing dampness, have strong antipyretic and fever-reducing, laxative and expectorant effects, and play a good inhibitory effect on influenza virus infection.

[0047] Studies have found that serum collected after oral administration of a Chinese medicine composition or pharmaceutical preparation has a good inhibitory effect on influenza virus infection. DETAILED DESCRIPTION

[0048] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.

[0049] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.

[0050] All medicinal materials have passed inspection and are in compliance with the standards of the Pharmacopoeia of the People's Republic of China. The times refer to the times of mass.

[0051] Example 1 Particles

[0052] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 250g of Artemisia annua, 250g of Scutellaria baicalensis, 375g of Poria cocos, 150g of dried tangerine peel, 150g of Rhizoma Anemarrhenae, 750g of Gypsum, 375g of Flos Lonicerae, 375g of Folium Isatidis, 250g of Pinellia ternata, 150g of Periostracum Cicadae, 150g of Menthus fragrans, 150g of Licorice and 50g of Indigo Naturalis.

[0053] This embodiment also provides a granule of the above-mentioned Chinese medicine composition, and its preparation method is as follows:

[0054] Take gypsum and add 6 times the amount of water, decoct for 30 minutes, collect the decoction and residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Honeysuckle, Folium Isatidis, Pinellia ternata, Periostracum Cicadae, Mentha, and Licorice, mix them, add A, add water to 11.5 times the weight of the prescribed medicinal materials (except Indigo), soak for 2 hours, decoct for 1.5 hours, filter the decoction, and collect the filtrate; add 10 times the amount of water to the residue, decoct for 1.5 hours, filter, combine the filtrate, and concentrate under reduced pressure to an extract with a relative density of 1.05-1.12 (60°C), spray-dry into fine powder, add the prescribed amount of Indigo and 5g stevioside, 40g citric acid, and an appropriate amount of dextrin, mix evenly, and dry granulate to make 1000g granules.

[0055] Example 2 Syrup

[0056] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 100g of Artemisia annua, 100g of Scutellaria baicalensis, 150g of Poria cocos, 100g of dried tangerine peel, 100g of Rhizoma Anemarrhenae, 580g of Gypsum, 200g of Flos Lonicerae, 200g of Folium Isatidis, 150g of Rhizoma Pinelliae, 100g of Periostracum Cicadae, 100g of Mint, 100g of Licorice and 20g of Indigo Naturalis.

[0057] This embodiment also provides a syrup of the above-mentioned Chinese medicine composition, and its preparation method is as follows:

[0058] Take gypsum and add 6 times the amount of water, decoct for 30 minutes, collect the decoction and residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Honeysuckle, Folium Isatidis, Pinellia ternata, Periostracum Cicadae, Mentha, Licorice, Indigo naturalis, mix, add A, add water to 11.5 times the weight of the prescribed medicinal materials, soak for 2 hours, decoct for 1.5 hours, filter the decoction, and collect the filtrate; add 10 times the amount of water to the residue, decoct for 1.5 hours, filter, combine the filtrate, and concentrate under reduced pressure to an extract with a relative density of 1.05-1.12 (60°C), take 2000g of sucrose, add water to boil, filter after dissolving, concentrate to make syrup, mix with the above concentrate, boil, cool, add preservatives and flavors, and dilute with appropriate amount of cold water to obtain syrup.

[0059] Example 3 Tablets

[0060] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 300g of Artemisia annua, 300g of Scutellaria baicalensis, 450g of Poria cocos, 270g of dried tangerine peel, 270g of Rhizoma Anemarrhenae, 820g of Gypsum, 450g of Flos Lonicerae, 450g of Folium Isatidis, 300g of Rhizoma Pinelliae, 270g of Periostracum Cicadae, 270g of Mentha, 270g of Licorice and 60g of Indigo Naturalis.

[0061] This embodiment also provides a tablet of the above-mentioned Chinese medicine composition, and its preparation method is as follows:

[0062] Take gypsum and add 6 times the amount of water, decoct for 30 minutes, collect the decoction and the residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Flos Lonicerae, Folium Isatidis, Rhizoma Pinelliae, Periostracum Cicadae, Mentha, and Licorice, mix them, add A, add water to 11.5 times the weight of the prescribed medicinal materials (except Indigo), soak for 2 hours, decoct for 1.5 hours, filter the decoction, and collect the filtrate; add 10 times the amount of water to the residue, decoct for 1.5 hours, filter, combine the filtrate, and concentrate under reduced pressure to an extract with a relative density of 1.05-1.12 (60°C), spray-dry into fine powder, add the prescribed amount of Indigo and an appropriate amount of dextrin, mix evenly, dry granulate, then add 0.5% magnesium stearate and 5% talc powder accounting for the mass of the particles, mix evenly, tablet, and coat to obtain.

[0063] Example 4 Micropellets

[0064] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 200g of Artemisia annua, 200g of Scutellaria baicalensis, 250g of Poria cocos, 170g of dried tangerine peel, 170g of Rhizoma Anemarrhenae, 650g of Gypsum, 350g of Flos Lonicerae, 250g of Folium Isatidis, 200g of Pinellia ternata, 170g of Periostracum Cicadae, 170g of Menthus fragrans, 170g of Licorice and 40g of Indigo Naturalis.

[0065] This embodiment also provides pellets of the above-mentioned Chinese medicine composition, and the preparation method thereof is as follows:

[0066] Take gypsum and add 6 times the amount of water, decoct for 30 minutes, collect the decoction and residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Flos Lonicerae, Folium Isatidis, Rhizoma Pinelliae, Periostracum Cicadae, Mentha, and Licorice, mix them, add A, add water to 11.5 times the weight of the prescribed medicinal materials (except Indigo), soak for 2 hours, decoct for 1.5 hours, filter the decoction, and collect the filtrate; add 10 times the amount of water to the residue, decoct for 1.5 hours, filter, combine the filtrate, and concentrate under reduced pressure to an extract with a relative density of 1.05-1.12 (60°C), spray-dry into fine powder, add the prescribed amount of Indigo and an appropriate amount of starch, mix evenly, use ethanol as a wetting agent, make micropills, and sieve; dry below 60°C to obtain micropills.

[0067] Example 5 Powder

[0068] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 12kg of Artemisia annua, 12kg of Scutellaria baicalensis, 18kg of Poria cocos, 7.2kg of Tangerine peel, 7.2kg of Rhizoma Anemarrhenae, 36kg of Gypsum, 18kg of Honeysuckle, 18kg of Folium Isatidis, 12kg of Pinellia ternata, 7.2kg of Periostracum Cicadae, 7.2kg of Menthus fragrans, 7.2kg of Licorice and 2.4kg of Indigo naturalis.

[0069] This embodiment also provides a powder of the above-mentioned Chinese medicine composition, and its preparation method is as follows:

[0070] Take gypsum and add 6 times the amount of water, boil for 30 minutes, collect the decoction and residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Flos Lonicerae, Folium Isatidis, Rhizoma Pinelliae, Periostracum Cicadae, Mentha, and Licorice, mix them, add A, add water to 11.5 times the weight of the prescribed medicinal materials (except Indigo), soak for 2 hours, boil for 1.5 hours, filter the decoction, and collect the filtrate; add 10 times the amount of water to the residue, boil for 1.5 hours, filter, combine the filtrate, and concentrate under reduced pressure to an extract with a relative density of 1.05-1.12 (60°C), spray dry into fine powder, and mix evenly with Indigo to obtain a powder.

[0071] Example 6 Powder

[0072] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 250g of Artemisia annua, 250g of Scutellaria baicalensis, 375g of Poria cocos, 150g of dried tangerine peel, 150g of Rhizoma Anemarrhenae, 750g of Gypsum, 375g of Flos Lonicerae, 375g of Folium Isatidis, 250g of Pinellia ternata, 150g of Periostracum Cicadae, 150g of Menthus fragrans, 150g of Licorice and 50g of Indigo Naturalis.

[0073] This embodiment also provides a powder of the above-mentioned Chinese medicine composition, and its preparation method is as follows:

[0074] Take gypsum and add 6 times the amount of 30% ethanol, decoct for 30 minutes, collect the decoction and residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Flos Lonicerae, Folium Isatidis, Rhizoma Pinelliae, Periostracum Cicadae, Mentha, and Licorice, mix them, add A, add 30% ethanol to 11.5 times the weight of the prescribed medicinal materials (except Indigo), soak for 2 hours, decoct for 1.5 hours, filter the decoction, and collect the filtrate; add 10 times the amount of 30% ethanol to the residue, decoct for 1.5 hours, filter, combine the filtrate, and concentrate under reduced pressure to an extract with a relative density of 1.05-1.12 (60°C), spray dry into fine powder, and mix evenly with Indigo to obtain a powder.

[0075] Example 7 Powder

[0076] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 250g of Artemisia annua, 250g of Scutellaria baicalensis, 375g of Poria cocos, 150g of dried tangerine peel, 150g of Rhizoma Anemarrhenae, 750g of Gypsum, 375g of Flos Lonicerae, 375g of Folium Isatidis, 250g of Pinellia ternata, 150g of Periostracum Cicadae, 150g of Menthus fragrans, 150g of Licorice and 50g of Indigo Naturalis.

[0077] This embodiment also provides a powder of the above-mentioned Chinese medicine composition, and its preparation method is as follows:

[0078] Take gypsum and add 6 times the amount of 50% ethanol, decoct for 30 minutes, collect the decoction and residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Flos Lonicerae, Folium Isatidis, Rhizoma Pinelliae, Periostracum Cicadae, Mentha, and Licorice, mix them, add A, add 50% ethanol to 11.5 times the weight of the prescribed medicinal materials (except Indigo), soak for 2 hours, decoct for 1.5 hours, filter the decoction, and collect the filtrate; add 10 times the amount of 50% ethanol to the residue, decoct for 1.5 hours, filter, combine the filtrate, and concentrate under reduced pressure to an extract with a relative density of 1.05-1.12 (60°C), spray dry into fine powder, and mix evenly with Indigo to obtain a powder.

[0079] Example 8 Powder

[0080] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 250g of Artemisia annua, 250g of Scutellaria baicalensis, 375g of Poria cocos, 150g of dried tangerine peel, 150g of Rhizoma Anemarrhenae, 750g of Gypsum, 375g of Flos Lonicerae, 375g of Folium Isatidis, 250g of Pinellia ternata, 150g of Periostracum Cicadae, 150g of Menthus fragrans, 150g of Licorice and 50g of Indigo Naturalis.

[0081] This embodiment also provides a powder of the above-mentioned Chinese medicine composition, and its preparation method is as follows:

[0082] Take gypsum and add 6 times the amount of 75% ethanol, decoct for 30 minutes, collect the decoction and residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Flos Lonicerae, Folium Isatidis, Rhizoma Pinelliae, Periostracum Cicadae, Mentha, and Licorice, mix them, add A, add 75% ethanol to 11.5 times the weight of the prescribed medicinal materials (except Indigo), soak for 2 hours, decoct for 1.5 hours, filter the decoction, and collect the filtrate; add 10 times the amount of 75% ethanol to the residue, decoct for 1.5 hours, filter, combine the filtrate, and concentrate under reduced pressure to an extract with a relative density of 1.05-1.12 (60°C), spray dry into fine powder, and mix evenly with Indigo to obtain a powder.

[0083] Example 9 Capsules

[0084] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 100g of Artemisia annua, 120g of Scutellaria baicalensis, 125g of Poria cocos, 100g of dried tangerine peel, 110g of Rhizoma Anemarrhenae, 650g of Gypsum, 275g of Honeysuckle, 225g of Folium Isatidis, 280g of Pinellia ternata, 250g of Periostracum Cicadae, 110g of Menthus fragrans, 120g of Licorice and 30g of Indigo Naturalis.

[0085] This embodiment also provides a powder of the above-mentioned Chinese medicine composition, and its preparation method is as follows:

[0086] Take gypsum and add 3 times the amount of 50% ethanol, decoct for 30 minutes, collect the decoction and residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Flos Lonicerae, Folium Isatidis, Rhizoma Pinelliae, Periostracum Cicadae, Mentha, and Glycyrrhizae, mix them, add A, add 50% ethanol to 10 times the weight of the prescribed medicinal materials (except Indigo), soak for 1.5 hours, decoct for 1 hour, filter the decoction, and collect the filtrate; add 10 times the amount of 50% ethanol to the residue, decoct for 1 hour, filter, combine the filtrate, and concentrate under reduced pressure to an extract with a relative density of 1.05-1.12 (60°C), spray-dry into fine powder, mix evenly with Indigo, granulate, and pack into capsules.

[0087] Embodiment 10 mixture

[0088] This embodiment provides a traditional Chinese medicine composition, comprising the following raw materials: 280g of Artemisia annua, 300g of Scutellaria baicalensis, 425g of Poria cocos, 260g of dried tangerine peel, 250g of Rhizoma Anemarrhenae, 800g of Gypsum, 425g of Flos Lonicerae, 425g of Folium Isatidis, 120g of Rhizoma Pinelliae, 110g of Periostracum Cicadae, 250g of Menthus, 270g of Licorice and 170g of Indigo Naturalis.

[0089] This embodiment also provides a powder of the above-mentioned Chinese medicine composition, and its preparation method is as follows:

[0090] Take gypsum and add 3 times the amount of water, decoct for 30 minutes, collect the decoction and residue to obtain A; take Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Flos Lonicerae, Folium Isatidis, Rhizoma Pinelliae, Periostracum Cicadae, Mentha, Glycyrrhizae, and Indigo Naturalis, mix them, add A, add water to 10 times the weight of the prescribed medicinal materials, soak for 2 hours, decoct for 1.5 hours, filter the decoction, and collect the filtrate; add 10 times the amount of water to the residue, decoct for 1 hour, filter, combine the filtrate, concentrate under reduced pressure to an appropriate amount, add an appropriate amount of ethanol for precipitation, take the supernatant, recover ethanol under reduced pressure, add water, stir, and let stand; take the supernatant, filter, concentrate the filtrate to 4000 ml, add an appropriate amount of preservative, stir well, and obtain.

[0091] Experimental Example 1 Study on the Anti-H1N1 Influenza Virus Effect of the Particles of the Present Invention in Vitro

[0092] 1. Experimental Materials

[0093] Oseltamivir: MedChemExpress, batch number: HY-17016-16759

[0094] The granules of the present invention are prepared according to the prescription and preparation method of Example 1 of the present invention.

[0095] Influenza A (H1N1) virus - wild type (H1N1-wild type), batch number: 20211127.

[0096] 2. Preparation of drug solution

[0097] Preparation of positive control drug: weigh an appropriate amount of oseltamivir and 2 Prepare 20 mM stock solution with 0.22 μm filter membrane for sterilization and store at 4 °C. Prepare the stock solution to the required concentration before use.

[0098] Preparation of drug-containing serum and blank serum in rats: Take an appropriate amount of the particles of the present invention, grind them evenly, add a small amount of ultrapure water to continue grinding, and finally adjust the volume to a test drug solution with a final concentration of 0.5g / mL with ultrapure water. Ten normal adult SPF-grade male SD rats were randomly divided into two groups according to body weight: a blank group (normal saline) and a particle (QLQ) group of the present invention, with 5 rats in each group. The rats in the particle group of the present invention were given the test drug solution by gavage at a dose of 25g / kg, and the blank control group was given an equal volume of normal saline (50ml / kg). Blood was collected from the femoral artery of the rats 1h after gavage, and the blood was allowed to stand at 5°C for 3h, centrifuged at 12000rpm for 10min, and the upper layer of light yellow transparent serum was taken, inactivated in a 56°C water bath for 30min, and sterilized by filtering with a disposable filter to prepare rat drug-containing serum and blank serum, respectively, and stored in a -80°C low-temperature refrigerator for standby use.

[0099] 3. Cytotoxicity assay

[0100] (1) Experimental methods

[0101] After 48 h of culture, the MDCK cells were basically grown into a monolayer. The cell culture medium was discarded, trypsin was added for digestion, and the cells were transferred to a 96-well sterile cell culture plate. 100 μL (0.2×10 5 cell / mL). Place in a cell culture incubator and culture for 24 hours to allow the cells to grow into a monolayer for later use. Dilute the rat drug-containing serum used in the experiment with cell maintenance medium (serum-free DMEM) to a volume ratio of 10% (volume ratio, that is, the volume of serum accounts for 10% of the total volume of serum and cell maintenance medium). Then dilute it with cell maintenance medium to form nine concentration gradients. Then add different concentrations of drug-containing serum to the cell culture wells that have discarded the cell culture medium, 100 μL per well, and repeat 6 wells for each concentration. At the same time, set up a cell control well (add cells but not drug-containing serum), 37°C, 5% CO 2 After culturing for 48 hours under the same conditions, add 10 μL of CCK-8 detection solution to each well and continue incubating for 1 hour. Select 450 nm wavelength, measure the light absorbance of each well on an enzyme-linked immunosorbent assay, record the results, and calculate the cell survival rate to find the maximum non-toxic concentration range of the drug to the cells.

[0102] (2) Experimental results

[0103] The results of the cell toxicity test showed that rat serum had no significant inhibitory effect on cell growth when the serum concentration was below 10%. Therefore, the experimental concentration of drug-containing serum was set at an initial concentration of 2% and then diluted.

[0104] 4. Antiviral Experiment

[0105] (1) Experimental methods

[0106] When the canine kidney cell line (MDCK) was basically full of monolayer after 48 h of culture, the cell culture medium was discarded, trypsin was added for digestion, and the cells were transferred to a 48-well sterile cell culture plate. 200 μL (0.2×10 5 Cells were placed in a cell culture incubator for 24 h to allow the cells to grow into a monolayer. The cells were randomly divided into a blank control group, a positive drug control group, a drug-containing serum group, a virus control group, and a blank serum control group.

[0107] The rat drug-containing serum group and the rat blank serum group were diluted with cell maintenance medium (2% DMEM culture medium containing serum) into six concentration gradients required for the experiment (the volume percentage of serum to the total volume of serum and cell maintenance medium was 2%, 1%, 0.50%, 0.25%, 0.125% and 0.0625%, respectively), and added to the culture plates with the supernatant discarded from the drug-containing serum group and the blank serum control group, 180 μL per well, and each concentration was repeated for 4 wells. The plates were placed in a cell culture incubator at 37°C and 5% CO. 2 Pretreatment for 12 hours. After the pretreatment, 100 μL (100 TCID50) of H1N1-wild-type strain diluted in cell maintenance solution was added to the cell culture wells of the drug-containing serum group, blank serum control group, virus control group and positive drug control group without the culture medium. After incubation for 1 hour, the virus solution was discarded, and the cells were washed twice with PBS. Then, rat drug-containing serum and rat blank serum were added to the corresponding culture wells of the rat drug-containing serum group and rat blank serum group at the previously diluted concentrations, 250 μL per well, and each concentration was repeated for 4 wells.

[0108] The positive drug control group was added with cell maintenance solution containing 200uM oseltamivir, 250μL per well, repeated for 4 wells;

[0109] The blank control group and the virus control group were only added with cell maintenance solution, 250 μL per well, and repeated for 4 wells. Each group was placed in a cell culture incubator at 37°C and 5% CO 2 Culture for 48h.

[0110] After 48 hours, the total cellular RNA was extracted using a viral DNA / RNA extraction kit, the viral RNA was reverse transcribed into cDNA, and then the viral content in the sample was detected by fluorescent quantitative PCR reaction, and the inhibition rate was calculated by the PCR semi-quantitative method.

[0111] The inhibition rate was calculated according to the following formula;

[0112] Inhibition rate (%) = [1-(relative virus content in rat drug-containing serum / relative virus content in rat blank serum control group)] × 100.

[0113] (2) Experimental results

[0114] Table 1 Results of drug-containing serum against H1N1-wild-type virus

[0115]

[0116] The results showed that 0.25%-2% of the drug-containing serum had a good inhibitory effect on influenza A virus H1N1-wild type.

[0117] Experimental Example 2 Study on the Anti-H3N2 Influenza Virus Effect of the Particles of the Present Invention in Vitro

[0118] 1. Experimental Materials

[0119] Oseltamivir: MedChemExpress. Batch number: HY-17016-16759

[0120] The granules of the present invention are prepared according to the prescription and preparation method of Example 1 of the present invention.

[0121] Influenza A(H3N2) virus (H3N2), from China Center for Type Culture Collection, number: GDV124, batch number: 20210126JP

[0122] 2. Preparation of drug solution

[0123] Same as Experimental Example 1

[0124] 3. Antiviral experiment

[0125] (1) Experimental methods

[0126] When the canine kidney cell line (MDCK) was basically full of monolayer after 48 h of culture, the cell culture medium was discarded, trypsin was added for digestion, and the cells were transferred to a 48-well sterile cell culture plate. 200 μL (0.2×10 5 Cells were placed in a cell culture incubator for 24 h to allow the cells to grow into a monolayer. The cells were randomly divided into a blank control group, a positive drug control group, a drug-containing serum group, a virus control group, and a blank serum control group.

[0127] The drug-containing serum and blank serum of rats were diluted with cell maintenance medium (DMEM culture medium containing 2% serum) to the six concentration gradients required for the experiment (the volume percentage of serum to the total volume of serum and cell maintenance medium was 2%, 1%, 0.50%, 0.25%, 0.125% and 0.0625%, respectively), and added to the culture plates of the drug-containing serum group and the blank serum control group, 180 μL per well, and each concentration was repeated for 4 wells. The plates were placed in a cell culture incubator at 37°C and 5% CO. 2 Pretreatment for 12 hours. After the pretreatment, 100 μL (100 TCID50) of H3N2 strain diluted in cell maintenance solution was added to the cell culture wells of the drug-containing serum group, blank serum control group, virus control group and positive drug control group without the culture medium. After incubation for 1 hour, the virus solution was discarded, and the cells were washed twice with PBS. Then, rat drug-containing serum and rat blank serum were added to the corresponding culture wells of the rat drug-containing serum group and rat blank serum group at the previously diluted concentrations, 250 μL per well, and each concentration was repeated for 4 wells.

[0128] The positive drug control group was added with cell maintenance solution containing 200uM oseltamivir, 250μL per well, repeated for 4 wells;

[0129] The blank control group and the virus control group were only added with cell maintenance solution, 250 μL per well, and repeated for 4 wells. Each group was placed in a cell culture incubator at 37°C and 5% CO 2 Culture for 48h.

[0130] After 48 hours, the total cellular RNA was extracted using a viral DNA / RNA extraction kit, the viral RNA was reverse transcribed into cDNA, and then the viral content in the sample was detected by fluorescent quantitative PCR reaction, and the inhibition rate was calculated by the PCR semi-quantitative method.

[0131] The inhibition rate was calculated according to the following formula;

[0132] Inhibition rate (%) = [1-(relative virus content in rat drug-containing serum / relative virus content in rat blank serum control group)] × 100.

[0133] (2) Experimental results

[0134] Table 2 Results of drug-containing serum against H3N2 virus

[0135]

[0136] The results showed that 0.25%-2% of the drug-containing serum had a good inhibitory effect on influenza A virus H3N2.

[0137] Experimental Example 3 Study on the effect of the particles of the present invention on H1N1 influenza virus oseltamivir-resistant strains in vitro

[0138] 1. Experimental Materials

[0139] Ribavirin: MedChemExpress. Batch number: HY-B0434-11970

[0140] The granules of the present invention are prepared according to the prescription and preparation method of Example 1 of the present invention.

[0141] H1N1 influenza virus oseltamivir-resistant strain (H1N1-antiOST, A / Puerto Rico / 8 / 1934), batch number: 20200402.

[0142] 2. Preparation of drug solution

[0143] Preparation of positive control drug: weigh an appropriate amount of ribavirin and 2 O was prepared into 1 mg / mL stock solution, filtered through a 0.22 μm filter membrane for sterilization, and stored at 4°C. It was prepared into the required concentration for the test before use.

[0144] Preparation of drug-containing serum and blank serum of rats: the same as in Experimental Example 1.

[0145] 3. Antiviral experiment

[0146] (1) Experimental methods

[0147] When the canine kidney cell line (MDCK) was basically full of monolayer after 48 h of culture, the cell culture medium was discarded, trypsin was added for digestion, and the cells were transferred to a 48-well sterile cell culture plate. 200 μL (0.2×10 5 cell / mL), and cultured in a cell culture incubator for 4 hours to allow the cells to grow into a monolayer. They were randomly divided into a blank control group, a positive drug control group, a drug-containing serum group, a virus control group, and a blank serum control group.

[0148] The drug-containing serum and blank serum of rats were diluted with cell maintenance medium (DMEM culture medium containing 2% serum) to the six concentration gradients required for the experiment (the volume percentage of serum to the total volume of serum and cell maintenance medium was 2%, 1%, 0.50%, 0.25%, 0.125% and 0.0625%, respectively), and added to the culture plates of the drug-containing serum group and the blank serum control group, 180 μL per well, and each concentration was repeated for 4 wells. The plates were placed in a cell culture incubator at 37°C and 5% CO. 2Pretreatment for 12 hours. After the pretreatment, the virus 100TCID50 / 100μL H1N1 influenza virus oseltamivir-resistant strain diluted in the cell maintenance solution was added to the cell culture wells with the medium discarded in the drug-containing serum group, blank serum control group, virus control group and positive drug control group. After incubation for 1 hour, the virus solution was discarded, and the cells were washed twice with PBS. Then, rat drug-containing serum and rat blank serum were added to the corresponding culture wells of the rat drug-containing serum group and rat blank serum group at the previously diluted concentrations, 250μL per well, and each concentration was repeated for 4 wells.

[0149] The positive drug control group was added with cell maintenance solution containing 25ug / ml ribavirin, 250μL per well, and repeated for 4 wells.

[0150] The blank control group and the virus control group were only added with cell maintenance solution, 250 μL per well, and repeated for 4 wells. Each group was placed in a cell culture incubator at 37°C and 5% CO 2 Culture for 48h.

[0151] After 48 hours, the total cellular RNA was extracted using a viral DNA / RNA extraction kit, the viral RNA was reverse transcribed into cDNA, and then the viral content in the sample was detected by fluorescent quantitative PCR reaction, and the inhibition rate was calculated by the PCR semi-quantitative method.

[0152] The inhibition rate was calculated according to the following formula;

[0153] Inhibition rate (%) = [1-(relative virus content in rat drug-containing serum / relative virus content in rat blank serum control group)] × 100.

[0154] (2) Experimental results

[0155] Table 3 Results of antiviral treatment of H1N1 influenza virus oseltamivir-resistant strains with drug-containing serum

[0156]

[0157] The results showed that 0.25%-2% of the drug-containing serum had a good inhibitory effect on oseltamivir-resistant strains of influenza A virus H1N1 influenza virus.

[0158] In summary, the Chinese medicine composition of the present invention has a good inhibitory effect on influenza A virus (H1N1-wild type, H3N2 and H1N1 influenza virus oseltamivir-resistant strains).

[0159] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. Use of a Chinese medicine composition in the preparation of an anti-influenza virus drug, It is characterized in that The Chinese medicine composition is composed of the following raw materials in parts by weight: 100-300 parts of Artemisia annua, 100-300 parts of Scutellaria baicalensis, 100-450 parts of Poria cocos, 100-270 parts of Tangerine peel, 100-270 parts of Rhizoma Anemarrhenae, 580-820 parts of Gypsum, 200-450 parts of Honeysuckle, 200-450 parts of Folium Isatidis, 100-300 parts of Pinellia ternata, 100-270 parts of Periostracum Cicadae, 100-270 parts of Mint, 100-270 parts of Licorice and 20-180 parts of Indigo Naturalis.

2. The use according to claim 1, It is characterized in that The Chinese medicine composition is composed of the following raw materials in parts by weight: 200-300 parts of Artemisia annua, 200-300 parts of Scutellaria baicalensis, 250-450 parts of Poria cocos, 150-270 parts of dried tangerine peel, 150-270 parts of Rhizoma Anemarrhenae, 700-820 parts of Gypsum, 300-450 parts of Honeysuckle, 300-450 parts of Folium Isatidis, 200-300 parts of Pinellia ternata, 150-270 parts of Periostracum Cicadae, 150-270 parts of Mint, 150-270 parts of Licorice and 50-150 parts of Indigo Naturalis.

3. The use according to claim 1, It is characterized in that The Chinese medicine composition is composed of the following raw materials in parts by weight: 100-150 parts of Artemisia annua, 100-150 parts of Scutellaria baicalensis, 150-200 parts of Poria cocos, 100-150 parts of Tangerine peel, 100-150 parts of Rhizoma Anemarrhenae, 580-700 parts of Gypsum, 200-300 parts of Honeysuckle, 200-300 parts of Folium Isatidis, 150-200 parts of Pinellia ternata, 100-150 parts of Periostracum Cicadae, 100-150 parts of Mint, 100-150 parts of Licorice and 40-50 parts of Indigo Naturalis.

4. The use according to claim 1, It is characterized in that The Chinese medicine composition is composed of the following raw materials in parts by weight: 250 parts of Artemisia annua, 250 parts of Scutellaria baicalensis, 375 parts of Poria cocos, 150 parts of Tangerine peel, 150 parts of Rhizoma Anemarrhenae, 750 parts of Gypsum, 375 parts of Honeysuckle, 375 parts of Folium Isatidis, 250 parts of Pinellia ternata, 150 parts of Periostracum Cicadae, 150 parts of Menthus fragrans, 150 parts of Licorice and 50 parts of Indigo Naturalis; or, The Chinese medicine composition is composed of the following raw materials: 100 parts of Artemisia annua, 100 parts of Scutellaria baicalensis, 150 parts of Poria cocos, 100 parts of Tangerine peel, 100 parts of Rhizoma Anemarrhenae, 580 parts of Gypsum, 200 parts of Honeysuckle, 200 parts of Folium Isatidis, 150 parts of Pinellia ternata, 100 parts of Periostracum Cicadae, 100 parts of Mint, 100 parts of Licorice and 20 parts of Indigo Naturalis; or, The Chinese medicine composition is composed of the following raw materials: 200 parts of Artemisia annua, 250 parts of Scutellaria baicalensis, 170 parts of Poria cocos, 170 parts of Tangerine peel, 170 parts of Rhizoma Anemarrhenae, 650 parts of Gypsum, 350 parts of Honeysuckle, 250 parts of Folium Isatidis, 200 parts of Pinellia ternata, 170 parts of Periostracum Cicadae, 170 parts of Mentha, 170 parts of Licorice and 120 parts of Indigo Naturalis; or, The Chinese medicine composition is composed of the following raw materials: 300 parts of Artemisia annua, 300 parts of Scutellaria baicalensis, 450 parts of Poria cocos, 270 parts of dried tangerine peel, 270 parts of Rhizoma Anemarrhenae, 820 parts of Gypsum, 450 parts of Honeysuckle, 450 parts of Folium Isatidis, 300 parts of Pinellia ternata, 270 parts of Periostracum Cicadae, 270 parts of Menthus fragrans, 170 parts of Licorice and 180 parts of Indigo Naturalis.

5. The use according to any one of claims 1 to 4, It is characterized in that The preparation method of the traditional Chinese medicine composition comprises the following steps: mixing Artemisia annua, Scutellaria baicalensis, Poria cocos, dried tangerine peel, Rhizoma Anemarrhenae, Gypsum, Honeysuckle, Folium Isatidis, Pinellia ternata, Periostracum Cicadae, Mentha arvense and Licorice, extracting the mixture according to a conventional extraction method or extracting the mixture separately according to a conventional extraction method and then mixing the mixture, and then mixing the mixture into Indigo Naturalis to obtain the traditional Chinese medicine composition; or mixing Artemisia annua, Scutellaria baicalensis, Poria cocos, dried tangerine peel, Rhizoma Anemarrhenae, Gypsum, Honeysuckle, Folium Isatidis, Pinellia ternata, Periostracum Cicadae, Mentha arvense ...lycyrrhiza uralensis and Indigo Naturalis, extracting the mixture according to a conventional extraction method or extracting the mixture separately according to a conventional extraction method and then mixing the mixture to obtain the traditional Chinese medicine composition.

6. The use according to claim 5, It is characterized in that The conventional extraction method includes one or more of decoction extraction, maceration extraction, percolation extraction, reflux extraction, ultrasonic extraction and steam distillation extraction; the extraction solvent is selected from water or an alcohol solution with a volume percentage of 5-98%; the number of extractions is at least 1; the extraction time is at least 10 minutes; the ratio of the mass of the extraction solvent to the weight of the raw material is ≥2.

7. The use according to any one of claims 1 to 4, It is characterized in that The Chinese medicine composition is added with or without a pharmaceutically acceptable carrier and prepared into a pharmaceutical preparation according to a conventional preparation method.

8. The use according to claim 7, It is characterized in that The pharmaceutical preparation is in the form of micropill, tablet, capsule, powder, mixture, granule, syrup, gel or decoction.

9. The use according to claim 7, It is characterized in that The pharmaceutically acceptable carrier is selected from at least one of pharmaceutically acceptable solvents, solubilizers, cosolvents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, glidants, flavoring agents, preservatives, suspending agents, coating materials, anti-adhesives, penetration enhancers, pH regulators and thickeners.

10. The use according to claim 7, It is characterized in that The preparation method of the pharmaceutical preparation comprises the following steps: Decoction step: taking gypsum and adding extraction solvent to decoct, collecting the decoction liquid and the residue to obtain A; taking Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Honeysuckle, Folium Isatidis, Pinellia ternata, Periostracum Cicadae, Mentha, and Licorice to mix, adding A, adding extraction solvent to soak, decoct and extract, separate the solid and liquid, collect the liquid, and obtain the liquid; The preparation steps of the pharmaceutical preparation are: concentrating and / or drying the medicinal solution, mixing it with Indigo Naturalis, adding or not adding auxiliary materials, and preparing the pharmaceutical preparation according to conventional processes; or, Decoction step: taking gypsum and adding extraction solvent to decoct, collecting the decoction liquid and the residue to obtain A; taking Artemisia annua, Scutellaria baicalensis, Poria cocos, Tangerine peel, Rhizoma Anemarrhenae, Honeysuckle, Folium Isatidis, Pinellia ternata, Periostracum Cicadae, Mentha, Licorice, Indigo naturalis, mixing, adding A, adding extraction solvent to soak, decoct and extract, separate solid and liquid, collect liquid, and obtain liquid; The preparation steps of the pharmaceutical preparation are: concentrating and / or drying the drug solution, adding or not adding auxiliary materials, and preparing the pharmaceutical preparation according to conventional processes.

11. The use according to any one of claims 1 to 4, It is characterized in that The influenza virus is influenza A virus.

12. The use according to claim 11, It is characterized in that The influenza A virus includes one or more of H1N1 and H3N2 viruses.

13. The use according to claim 12, It is characterized in that The H1N1 virus includes an H1N1 wild type or an H1N1 influenza virus oseltamivir-resistant strain.

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