A high-efficiency mobile sample detection all-in-one machine and a control method thereof

Abstract

The application discloses a high-efficiency mobile sample detection all-in-one machine and a control method thereof, relates to the field of medical instruments, and provides the high-efficiency mobile sample detection all-in-one machine and the control method thereof, which divides the all-in-one machine into an upper region driven by an upper driving unit and a lower region driven by a lower driving unit, moves through cooperation of the upper driving unit and the lower driving unit, reduces waiting time in the detection process, and improves detection efficiency and detection accuracy; high-precision low-speed driving and low-precision high-speed driving are cooperated with each other, so that the moving process can have high speed and high precision.

Description

Technical Field

[0001] This invention relates to the field of medical devices, specifically to a highly efficient mobile integrated sample testing machine and its control method. Background Technology

[0002] In vitro diagnostic technology has developed into an important means of analyzing various samples of organisms, such as blood, feces, and swabs. In this detection technology, nucleic acids, as carriers of the organism's genetic information, require isolation, purification, amplification, and detection, whether for studying nucleic acid sequences and structures or exploring gene functions and expression. Therefore, the purification and quantitative or qualitative analysis of nucleic acids are essential.

[0003] In existing technologies, nucleic acid detection requires a number of processing steps, and the reagents, reaction solutions, and environments required for these steps often vary. Therefore, the optimal choice is to perform different reactions in different consumable wells. This requires moving pipetting modules, extraction modules, etc., to perform corresponding operations between different wells to automate the transfer, extraction, purification, and establishment of amplification systems for nucleic acids.

[0004] For example, nucleic acid extraction or other related nucleic acid reactions involve sample transfer, lysis, washing, and elution. A pipetting module is needed to transfer the sample solution from the sample tube to the extraction consumables. These extraction consumables are equipped with pipetting wells, reserved wells, lysis wells, magnetic bead preservation wells, washing wells, elution wells, magnetic rod sleeve wells, and elution buffer wells. The extraction module moves between these wells to purify and elute the sample. Then, the elution buffer is transferred to the amplification consumables via a pipetting module. These amplification consumables are equipped with premixing wells, sealing liquid wells, and multiple amplification wells. The pipetting module moves between these wells to establish the amplification system. Ideally, all these processes would be performed as a fully automated, integrated analysis with sample input and result output. Driven by this demand, designing integrated consumables and corresponding integrated equipment has become a design goal for an increasing number of manufacturers.

[0005] Chinese invention patents CN107727874B and CN106996984B protect a purely integrated consumable design that combines pipetting consumables, extraction consumables, and PCR amplification consumables into one, creating a consumable design adaptable to different needs of sample extraction and amplification. However, this design has a limited number of amplification wells, making it difficult to perform detection of multiple targets, and the consumables are difficult to seal after use, posing a risk of contamination. Chinese invention patent CN106148512B, entitled "Scanning Real-Time Microfluidic Thermal Cyclist and Method for Synchronous Thermal Cycling and Scanning Optical Detection," proposes a scheme using microfluidic consumables to perform extraction and amplification detection. While this design can operate in a closed state, reducing the risk of system contamination, its consumable manufacturing is relatively complex. Small-sized microchannels, especially those below 0.5 mm, require consideration of capillary forces, surface tension, and other influencing factors, significantly increasing the complexity of the channel design. In special cases, further processing may be necessary. Microfluidic valves need to be configured to constrain the fluid flow range; Chinese invention patent application CN114364811A, invention title: Sample preparation device and multi-well plate with PCR chip, discloses an integrated consumable design where the extraction consumable is designed as a traditional large-capacity structure, while the amplification consumable is configured to be connected to the elution wells through a microfluidic channel. This design requires reliable sealing between the amplification wells and the extraction / elution wells after the elution buffer transfer is completed; otherwise, thermal cycling amplification will cause liquid backflow and cross-contamination under temperature-driven conditions. However, for isothermal amplification schemes, this design can rely on the bending of the capillary length to ensure that there is no risk of backflow or cross-contamination between the amplification chamber and the extraction / elution wells; the scheme authorized by US invention patent US9857384B2 designs the reagent kit as a divisible type, which can be processed and produced according to different production processes during the manufacturing stage, and different reagent kits can be spliced ​​and combined for use with an integrated instrument during the actual use stage. Based on current technology, integrated detection equipment increasingly needs to perform more multi-target analyses, and adopting segmented large-size consumable designs can achieve the analysis goal of sample input and result output at a lower cost. However, integrated instruments with multiple detection and higher throughput mean that the travel range is too long. In this case, it is also necessary to balance sample processing speed and the accuracy of different processing steps.

[0006] Existing integrated sample testing devices typically use a single drive source to move the pipetting and extraction modules. This single drive source only provides one movement speed and one level of precision. If high-precision movement is desired, the movement speed will be reduced, resulting in low detection efficiency and long waiting times during nucleic acid testing, which is detrimental to the accuracy of the test. Conversely, increasing the movement speed will reduce the precision, easily leading to problems such as misalignment of wells, which will affect the test results. Summary of the Invention

[0007] The purpose of this invention is to address the aforementioned problems by providing an efficient mobile sample detection all-in-one machine and its control method. The all-in-one machine is divided into an upper region driven by an upper drive unit and a lower region driven by a lower drive unit. By coordinating the movement of the upper and lower drive units, the movement process can achieve both high speed and high precision, thereby improving detection efficiency and accuracy and adapting to high-throughput, multi-detection application scenarios.

[0008] The technical solution adopted in this invention is as follows:

[0009] A highly efficient mobile sample testing all-in-one machine includes an upper region and a lower region. The upper region includes an upper drive unit and a combination module. The upper drive unit can drive the combination module to move horizontally. The combination module includes a pipetting module and an extraction module. The lower region includes a lower drive unit and a support platform. The support platform includes a sample tube area, an extraction consumable area, and an amplification consumable area. The lower drive unit can drive the support platform to move horizontally. The time period during which the upper drive unit drives the combination module to move is a first time period, and the time period during which the lower drive unit drives the support platform to move is a second time period. There is an overlap between the first time period and the second time period. The upper drive unit and the lower drive unit cooperate with each other, enabling the pipetting module to move between the sample tube area and the extraction consumable area to perform sample transfer operations, the extraction module to move within the extraction consumable area to perform sample extraction and purification operations, the pipetting module to move between the extraction consumable area and the amplification consumable area to perform elution transfer operations, and the pipetting module to move within the amplification consumable area to perform solution dispensing operations.

[0010] Furthermore, the upper drive unit drives the assembly module with a first precision, and the assembly module moves horizontally at a first speed under the action of the upper drive unit. The lower drive unit drives the support platform to move horizontally with a second precision, and the support platform moves horizontally at a second speed under the action of the lower drive unit. The first precision is higher than the second precision and the first speed is lower than the second speed, or the first precision is lower than the second precision and the first speed is higher than the second speed.

[0011] Furthermore, the lower drive unit includes a first motor and a second motor. The first motor can drive the support platform to move within a first movement distance, and the second motor can drive the support platform to move within a second movement distance. The first motor and the second motor cooperate with each other to ensure that the movement distance of the support platform does not exceed the sum of the first movement distance and the second movement distance. The first motor and the second motor can cooperate with each other to drive the support platform to perform loading and configuration operations outside the housing. The first motor and the second motor work in series.

[0012] Furthermore, the combined module also includes an identification module, and the upper driving unit can cooperate with the lower driving unit so that the first time period of the corresponding identification module's movement and the second time period of the corresponding carrier platform's movement at least partially overlap; the consumable identification camera of the identification module can perform dynamic barcode scanning or static barcode scanning.

[0013] Furthermore, the pipetting module can transfer and drain liquid at a first draining speed and mix and drain liquid at a second draining speed, wherein the first draining speed is 1 / 4 to 1 / 2 of the second draining speed.

[0014] A control method for a high-efficiency mobile sample testing all-in-one machine, employing the aforementioned high-efficiency mobile sample testing all-in-one machine, includes the following steps:

[0015] Sample transfer steps: The upper drive unit drives the pipetting module to move in the first time period, and the lower drive unit drives the stage to move in the second time period. The first and second time periods overlap at least partially. The upper and lower drive units cooperate with each other to drive the pipetting module to move between the sample tube and the lysis well of the extraction consumable to perform the sample transfer operation.

[0016] Sample extraction and purification steps: The upper drive unit drives the extraction module to move in the first time period, and the lower drive unit drives the stage to move in the second time period. The first and second time periods overlap at least partially. The upper and lower drive units cooperate with each other to drive the extraction module to move between the wells of the extraction consumables to perform sample extraction and purification operations.

[0017] The amplification system setup steps are as follows: The upper drive unit drives the pipetting module to move during the first time period, and the lower drive unit drives the stage to move during the second time period. The first and second time periods overlap at least partially. The upper and lower drive units cooperate to drive the pipetting module to move between the elution wells of the extraction consumables and the premixing wells of the amplification consumables to perform the elution transfer operation. The pipetting module performs n mixing operations in the premixing wells, where n is a positive integer. The mixing operation involves the pipetting module drawing the elution from the elution wells and then discharging the elution into the premixing wells. After the elution is mixed with the lyophilized reagent, the upper and lower drive units cooperate to drive the pipetting module to move between the wells of the amplification consumables to perform the solution dispensing operation.

[0018] Furthermore, in the amplification system setup step, the pipetting module transfers the elution buffer from the elution well of the extraction consumable to the premixing well of the amplification consumable in m steps, where m is an integer greater than or equal to 2.

[0019] Furthermore, in the amplification system establishment step, the pipetting module transfers and discharges liquid at a first discharge rate during the pipetting process and mixes and discharges liquid at a second discharge rate during the mixing operation. The first discharge rate is 1 / 4 to 1 / 2 of the second discharge rate.

[0020] Furthermore, the process includes steps prior to the sample transfer step.

[0021] Loading configuration steps: The first motor and the second motor in the lower drive unit work in series to drive the carrier stage outside the housing, load sample tubes in the sample tube area, load extraction consumables in the extraction consumables area, and load amplification consumables in the amplification consumables area. After loading is completed, the first motor and the second motor work in series to drive the carrier stage back into the housing.

[0022] Furthermore, the process after loading the configuration step also includes...

[0023] The scanning and recognition process is as follows: the upper drive unit drives the recognition module to move during the first time period, and the lower drive unit drives the carrier platform to move during the second time period. The first and second time periods overlap at least partially. The recognition module scans and recognizes the extracted consumables and the amplified consumables respectively. When the upper and lower drive units drive simultaneously, the consumable recognition camera of the recognition module performs dynamic scanning. When the upper drive unit drives alone, the consumable recognition camera of the recognition module performs static scanning.

[0024] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0025] 1. The present invention reduces the waiting time during the detection process and improves the detection efficiency and accuracy by having the upper drive unit and the lower drive unit move in coordination.

[0026] 2. The present invention employs a combination of high-precision low-speed drive and low-precision high-speed drive, enabling the movement process to achieve both high speed and high precision.

[0027] 3. The lower drive unit of the present invention is a dual-motor mechanism, which can achieve longer and more stable movement within a limited space, thereby realizing the miniaturization of the integrated machine mechanism.

[0028] 4. The first motor and the second motor of the lower drive unit of the present invention work in series to avoid adverse factors such as resonance or noise superposition when the two motors drive the same support platform at the same time.

[0029] 5. This invention can perform barcode scanning and recognition efficiently and quickly.

[0030] 6. In this invention, the eluent is added in several stages during the transfer process to avoid adding too much eluent at once, which would prevent the lyophilized reagent from dissolving immediately and causing the eluent to overflow.

[0031] 7. The pipetting module of this invention uses a low discharge rate during the pipetting process to ensure that no air bubbles are generated during pipetting and separation. It uses a high discharge rate during the mixing operation to make the eluent and lyophilized reagent mix efficiently and more thoroughly. Attached Figure Description

[0032] Figure 1 This is a schematic diagram of the functional modules of the all-in-one machine of the present invention;

[0033] Figure 2 This is a schematic diagram of the internal structure of the all-in-one machine of the present invention;

[0034] Figure 3 This is a schematic diagram of the internal structure of the all-in-one machine in another state of the present invention;

[0035] Figure 4 This is a schematic diagram of the structure of the lower drive unit of the present invention;

[0036] Figure 5 This is a schematic diagram of the structure of the support platform and the lower drive unit of the present invention.

[0037] Figure 6 This is a schematic diagram of the driving principle of the lower driving unit of the present invention;

[0038] Figure 7 This is a schematic diagram of the overall appearance structure of the all-in-one machine of the present invention;

[0039] Figure 8 This is a schematic diagram illustrating the sample solution transfer principle of the present invention;

[0040] Figure 9 This is a schematic diagram illustrating the principle of the nucleic acid extraction and purification operation performed by the extraction consumables of this invention;

[0041] Figure 10 This is a schematic diagram illustrating the eluent transfer principle of the present invention;

[0042] Figure 11 This is a schematic diagram illustrating the principle of the amplification system establishment operation performed by the amplification consumables of this invention;

[0043] Figure 12 This is a schematic diagram of the sample tube opening and closing mechanism of the present invention;

[0044] Figure 13 This is a schematic diagram of the pipetting module of the present invention;

[0045] Figure 14 This is a schematic diagram of the extraction module of the present invention;

[0046] Figure 15 This is a schematic diagram of the second and fourth air ducts of the present invention;

[0047] Figure 16This is the control timing diagram of the all-in-one machine of the present invention;

[0048] Figure 17 This is a block diagram of the control module of the all-in-one machine of the present invention.

[0049] In the diagram, the markings are as follows: 20 - Sample tube cap switching mechanism, 30 - Extraction consumable cap switching mechanism, 40 - Identification module, 50 - Pipette module, 60 - Extraction module, 70 - Amplification consumable cap switching mechanism, 101 - First motor, 102 - First lead screw, 103 - First slider, 104 - First platform, 105 - First guide rail, 106 - Slide assembly, 201 - Second motor, 202 - Second lead screw, 203 - Second slider, 204 - Support platform, 205 - Sliding cavity, 207 - Second guide rail, 208 - Slide unit. 301-Housing, 302-Opening and closing part, 303-Automatic loading area, 304-Sample tube area, 305-Extraction consumable area, 306-Amplification consumable area, 307-Heat sink, 308-Bottom fan, 309-Exhaust channel, 310-Optical detection module, 315-Third motor, 316-Third lead screw, 801-First vent, 802-Second vent, 8021-Independent air duct, 8022-Top fan, 803-Third vent, 8031-Middle fan, 804-Fourth vent. Detailed Implementation

[0050] The present invention will now be described in detail with reference to the accompanying drawings.

[0051] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0052] Example 1

[0053] A highly efficient, mobile, all-in-one sample testing machine, such as... Figure 1-15As shown, the system includes an upper region and a lower region. The upper region includes an upper drive unit and a combined module. The upper drive unit can drive the combined module to move horizontally. The combined module includes a pipetting module 50 and an extraction module 60. The lower region includes a lower drive unit and a support platform 204. The support platform 204 includes a sample tube area 304, an extraction consumable area 305, and an amplification consumable area 306. The lower drive unit can drive the support platform 204 to move horizontally. The time period during which the upper drive unit drives the combined module to move is a first time period. The time period during which the lower drive unit drives the combined module to move is a first time period. The time period during which the support platform 204 moves is the second time period. There is an overlap between the first time period and the second time period. The upper drive unit and the lower drive unit cooperate with each other, so that the pipetting module 50 can move between the sample tube area 304 and the extraction consumable area 305 to perform sample liquid transfer operations, the extraction module 60 can move within the extraction consumable to perform sample extraction and purification operations, the pipetting module 50 can move between the extraction consumable area 305 and the amplification consumable area 306 to perform elution buffer transfer operations, and the pipetting module 50 can move within the amplification consumable area 306 to perform solution dispensing operations.

[0054] The pipetting module 50 enables the transfer of samples or solutions containing nucleic acid fragments. The module includes a pipette, a pipette drive motor for adjusting the pipette's position by moving it up and down, and a working motor for driving the pipetting push rod. The pipette adjusts the relative position of the pipetting push rod and the mating chamber to change the internal pressure of the consumable, thereby performing aspiration and dispensing operations. The pipette can be shared for both sample and nucleic acid fragment solutions by using different pipetting tips at different stages. In other embodiments, independent pipettes can be used to pipette samples or solutions containing nucleic acid fragments separately. For example, a design with eight pipetting tips can achieve parallel processing of more sample aspiration operations, see reference [reference needed]. Figure 13 The specific quantity is not limited here.

[0055] The extraction module 60 can achieve the functions of stirring and adsorbing nucleic acid fragments in a solution containing nucleic acid fragments and transferring the nucleic acid fragments. Specifically, it can be a magnetic stirring mounting part and a magnetic rod sleeve. The magnetic stirring mounting part and magnetic rod sleeve achieve the effect of nucleic acid extraction and stirring. Stirring can be achieved by up-and-down oscillation or rotation stirring, such as... Figure 14 As shown, this uses a composite motion scheme that combines the rotational motion of eight magnetic stirring mounting parts driven by an independent drive motor with the up-and-down oscillation motion driven by a motor. This is not a limitation.

[0056] The upper drive unit drives the assembly module with a first precision, and the assembly module moves horizontally at a first speed under the action of the upper drive unit. The lower drive unit drives the support platform 204 to move horizontally with a second precision, and the support platform 204 moves horizontally at a second speed under the action of the lower drive unit. The first precision is higher than the second precision and the first speed is lower than the second speed.

[0057] The lower drive unit includes a first motor 101 and a second motor 201. The first motor 101 can drive the support platform 204 to move within a first movement distance, and the second motor 201 can drive the support platform 204 to move within a second movement distance. The first motor 101 and the second motor 201 cooperate to ensure that the movement distance of the support platform 204 does not exceed the sum of the first movement distance and the second movement distance. An opening and closing part 302 is provided on the housing 301. When the opening and closing part 302 is open, the first motor 101 and the second motor 201 can cooperate to drive the support platform 204 outside the housing 301, so that the automated loading area 303 in the support platform 204 is completely exposed outside the coverage area of ​​the housing 301 for loading and configuration operations. The automated loading area 303 includes a sample tube area 304, an extraction consumable area 305, and an amplification consumable area 306. These areas are arranged in a parallel line along the direction of the inlet / outlet opening / closing section 302, allowing for the detection of more targets within a limited space and ensuring high instrument integration. The automated loading area 303 may contain several parallel loading units, illustrated here as eight. Each loading unit includes one sample tube, one extraction consumable, and one amplification consumable. The parallel loading units enable multi-target multiplex detection of up to eight samples. The sample tube is placed in the sample tube area 304 in a closed state, the extraction consumable is placed in the extraction consumable area 305 in a closed state, and the amplification consumable is placed in the amplification consumable area 306 in a closed state. This ensures that the loading process does not pose a risk of contamination to the operator, resulting in higher operational safety.

[0058] In this embodiment, the principle of the first motor and the second motor is as disclosed in application number 202211094300.1, which describes a dual-motor drive structure and its driven nucleic acid extraction and detection integrated machine. The support platform 204 is connected to the first slider 103, so that when the first slider 103 moves along the length direction of the first lead screw 102, the first slider 103 can drive the support platform 204 to move along the length direction of the first lead screw 102. The first slider 103 can be sleeved on the first lead screw 102. The first lead screw 102 is fixed on the first platform 104 through a bearing seat. The first motor 101 is connected to the first lead screw 102 through a coupling and / or an encoder. When the first motor 101 rotates, it drives the first lead screw 102 to rotate synchronously between the two bearing seats, thereby achieving precise movement of the first slider 103 on the first lead screw 102.

[0059] A first guide rail 105 may be provided on the first platform 104. A slide rail assembly 106 matches and connects the support platform 204 to the first guide rail 105. The slide rail assembly 106 directly or indirectly connects the support platform 204 to the first slider 103. For example, it can be fixedly connected by a sheet-like connector, so as to drive the support platform 204 to move freely along the length direction of the first guide rail 105.

[0060] The second slider 203 can move along the length direction of the second lead screw 202, such that the limit absolute movement distance of the second slider 203 is greater than the distance that the first slider 103 can move on the first lead screw 102 or the distance that the second slider 203 can move on the second lead screw 202, but does not exceed the sum of the two distances.

[0061] The second motor 201 can be located at the bottom of the support platform 204. The bottom of the support platform 204 is provided with a bearing seat for fixing the second lead screw 202. The second motor 201 drives the second lead screw 202 through a pulley, or the second motor 201 drives the second lead screw 202 through a gear, so that the second motor 201 drives the second lead screw 202 to rotate at the bottom of the support platform 204, thereby enabling the second slider 203 sleeved on the second lead screw 202 to move accurately.

[0062] The support platform 204 may be provided with a sliding cavity 205 for the second slider 203 to move, and the second slider 203 is fixed within the sliding cavity 205. A second guide rail 207 is provided at the bottom of the support platform 204, and a slide groove unit 208 matching the second guide rail 207 is provided on the slide groove assembly 106. The slide groove unit 208 cooperates with the second guide rail 207 to provide guidance for the movement of the support platform 204. When the second slider 203 moves along the length direction of the second lead screw 202, the second guide rail 207 and the slide groove unit 208 of the support platform 204 move relative to each other in the length direction of the second guide rail 207. The two relatively arranged second guide rails 207 and two slide groove units 208 enable the support platform 204 to move stably under the drive of the second motor 201.

[0063] The control method for the first motor 101 and the second motor 201 can be that the first motor 101 starts first, and then the second motor 201 starts. That is, the first slider 103 first drives the carrier platform 204 to move to the first limit distance, and then the second slider 203 drives the carrier platform 204 to move to the preset limit distance. The first motor 101 and the second motor 201 work in series to avoid adverse factors such as resonance or noise superposition when the two motors drive the same carrier platform at the same time.

[0064] The slide rail assembly 106 enables the relative motion driven by different drive units to be linked in series with the same reference, ensuring higher design reliability. Specifically, when the first motor 101 drives the first slider 103 to move through the first lead screw 102, the slide rail assembly 106 drives the entire support platform 204 to move along the length direction of the first guide rail 105 under the guidance of the first guide rail 105. When the second motor 201 drives the second slider 203 to move through the second lead screw 202, the movement state of the slide rail unit 208 is the same as that of the slide rail assembly 106. If the first motor 101 is not working, the slide rail unit 208 is stationary, while the second slider 203 drives the second guide rail 207 of the support platform to move. In this way, a reliable dual-motor series relay motion drive is achieved by using the same slide rail assembly 106 to drive two different drive mechanisms.

[0065] The first motor 101 on the first platform 104 drives the first slider 103 via the first lead screw 102, enabling free movement within a length range of, for example, D10. In actual implementation, due to positioning requirements, the actual range of motion is shorter than the theoretical entire length of the lead screw 102's threaded section. Position sensors, such as photoelectric switches, can be positioned at predetermined locations. The second motor 201 on the support platform 204 drives the second slider 203, enabling free movement within a length range of, for example, D20. Similarly, the actual range of motion of the second lead screw 202 is relatively short. Ultimately, the dual-motor drive mechanism, composed of the same slide rail assembly 106, allows the second slider 203 to reach an absolute limit movement distance of D30, exceeding the distance the first slider 103 can move on the first lead screw 102 or the distance the second slider 203 can move on the second lead screw 202, but not exceeding the sum of these distances. This ensures that the dual motors achieve a larger range of motion while maintaining stability and reliability.

[0066] The upper drive unit includes a third motor 315 and a third lead screw 316.

[0067] The pitch of the third lead screw 316 is smaller than that of the first lead screw 102 and / or the second lead screw 202 to ensure the accuracy requirements of operation.

[0068] The all-in-one machine also includes an extraction consumable switch cover mechanism 30 for opening and closing extraction consumables, an amplification consumable switch cover mechanism 70 for opening and closing amplification consumables, and a sample tube switch cover mechanism 20 for opening and closing sample tubes.

[0069] The extraction consumables and the extraction consumables switching cover mechanism 30 can adopt existing technical structural designs, such as the nucleic acid reaction consumables switching cover mechanism disclosed in application number CN111847343A. The extraction consumables switching cover mechanism 30 includes a first structural layer, a second structural layer, and a third structural layer. Several guide rods 1 are vertically arranged below the first structural layer. The upper end of the guide rods 1 is connected to the first structural layer, and the lower end of the guide rods 1 passes through the second and third structural layers. Several guide rods 2 are vertically arranged between the second and third structural layers. The upper end of the guide rods 2 passes through the second structural layer, and the lower end of the guide rods 2 is connected to the third structural layer. An elastic component is sleeved on the guide rods 2. The first structural layer is connected to the second structural layer through a lifting device, which is used to drive the second structural layer to move up and down along the guide rods 1. A consumables cover fixing mechanism is provided on the second structural layer to fix the consumables cover on the second structural layer. A consumables body fixing mechanism is provided on the third structural layer to restrict the movement of the consumables body.

[0070] The consumable retrieval system comprises two parts: a consumable body and a consumable cover. The consumable body includes a connecting plate and several tubes inserted into the connecting plate. Each tube has an opening, and all tube openings are on the same plane. The width of the connecting plate is greater than the diameter of the tube opening to form a first flange on the outer wall of the tube for fixing the tube when the cover is opened. The consumable cover includes several tube caps that are paired with the tubes to block the tube openings and are connected to the same side of the tube cap plate. The edge of the tube cap has a second flange that serves as a force point when the tube cap is pulled off the tube. When the tube cap is closed on the tube, a gap is formed between the second flange and the first flange, which facilitates the insertion of the consumable retrieval cover opening mechanism 30 through this gap, thereby realizing the cover opening operation.

[0071] The extraction consumables are 10-well tubing consumables, arranged from near to far from the sample tube as follows: first pipetting consumable well, reserved well, lysis well, magnetic bead preservation well, wash A well, wash B well, wash C well, elution well, magnetic rod sleeve well, and second pipetting consumable well. The washing solution in wash A well can be 600-700 μL, the washing solution in wash B well can be 650-750 μL, the washing solution in wash C well can be 750-850 μL, and the eluent in the elution well can be 150-250 μL, to perform more thorough washing and elution operations. When the target sample is bacteria, the reserved well contains proteinase K reagent. The pipetting module 50 can transfer the sample solution into the reserved well to dissolve and destroy the protein capsid. Then, the pipetting module 50 can transfer the solution from the reserved well to the lysis well, enabling the integrated instrument to handle both bacterial and viral samples, thus enhancing its compatibility. The lysis well, magnetic bead preservation well, and elution well are all equipped with heating units. Heating in the lysis well is compensated for by the adjacent magnetic bead preservation well, resulting in more complete lysis, improved nucleic acid fragment yield, and guaranteed detection accuracy. The heating unit in the magnetic bead preservation well does not affect the physical properties of the magnetic beads and does not adversely affect the overall reaction. In actual use, some wells of the tube extraction consumable can be combined or separated to form 9-well, 8-well, 11-well, and 12-well tube consumables. However, to ensure extraction efficiency, the number of wells should not be less than 8. In this design, the 10-well pipette consumable has two ends, one for a first pipette consumable and the other for a second pipette consumable, each adapted to different capacities. The capacity of the first pipette consumable (for transferring sample solution) can be configured to be more than twice that of the second pipette consumable (for transferring elution buffer). This ensures sufficient and efficient transfer of sample solution, while allowing for precise and minute-level transfer of elution buffer and PCR premix, thus adapting to various transfer needs. Figure 8As shown, the pipetting consumable near the sample tube is a sample liquid pipetting consumable with a first capacity, used in conjunction with the pipetting module 50 to transfer the sample liquid from the sample tube to the lysis well of the extraction consumable. During this process, the lower drive unit can drive the support stage 204 to move horizontally. Here, the first motor 101 of the lower drive unit can drive the support stage 204 to move horizontally via the first lead screw 102. The upper drive unit drives the combined module during the first time period, which overlaps with the second time period driven by the lower drive unit, thereby driving the pipetting module 50 to quickly and accurately perform the operation of transferring the sample liquid to the lysis well. After the operation is completed, the sample liquid is transferred... The liquid consumables are recycled into the first pipetting consumable well. During this process, the top fan 8022 can operate continuously. Combined with the overlapping time periods of the upper and lower drive units, this enables rapid switching of the extraction environment, reducing the risk of contamination. It also shortens the exposure time of the sample liquid pipetting consumables that have come into contact with the sample liquid, further reducing the risk of contamination. The end of the magnetic rod sleeve, furthest from the sample liquid pipetting consumable, is positioned in the 10-well connecting tube consumable, furthest from the first pipetting consumable well and adjacent to the second pipetting consumable well. The second pipetting consumable well contains a second-capacity elution buffer pipetting consumable, used to transfer the elution buffer to the amplification consumables. Figure 9 a illustrates how the extraction module 60 moves to the magnetic rod sleeve hole to connect with the magnetic rod sleeve, and then moves to the magnetic bead storage hole. Figure 9 The diagram (b) illustrates the process of a magnetic rod descending and extending into the magnetic rod sleeve, adsorbing magnetic beads from the magnetic bead storage wells onto the magnetic rod sleeve. The extraction module 60 then transfers the magnetic beads to the lysis wells, and vice versa. During this process, the upper and lower driving units can overlap in time, driving the combined module and the support stage 204 respectively, enabling efficient and low-contamination-risk adsorption of nucleic acid fragments. Figure 9 c illustrates the vibration and rotation of the magnetic rod sleeve at the pyrolysis site to mix the particles. Figure 9 d illustrates the adsorption and lysis of nucleic acid fragments by magnetic beads, and the extraction module transfers 60 of these fragments to the washing well A. Figure 9 e illustrates the washing and purification of nucleic acid fragments in the washing well A. Figure 9 f indicates that the extraction module 60 is moved to the washing port B. Figure 9 g indicates that nucleic acid fragments are washed and purified in the washing well B. Figure 9 h indicates that the extraction module 60 is transferred to the washing port C. Figure 9 i illustrates the washing and purification of nucleic acid fragments in the C-well. Figure 9 j indicates that the extraction module 60 is transferred to the elution well, which completes the process for certain viruses. Figure 9 After washing and purifying g, proceed directly. Figure 9 j is transferred to the elution well position without performing [executive] Figure 9 h、 Figure 9 i, Figure 9 k indicates that the release of nucleic acid fragments is completed within the elution well. Figure 9 The diagram illustrates the process of transferring magnetic beads to the magnetic bead storage well according to step S1, and placing the magnetic rod sleeve back into the magnetic rod sleeve well according to step S2. This completes the entire process of sample extraction and purification using the magnetic bead method in conjunction with the 10-well tubing consumable. Throughout the entire operation, the extraction and purification process using the magnetic rod sleeve and the sample transfer process using the sample liquid transfer consumable can proceed without overlapping displacement, reducing the risk of cross-contamination during the extraction process due to sample evaporation.

[0072] The principle of the amplification consumable is as disclosed in a multiplex PCR detection kit and method in application number 202211518935.X. The amplification consumable includes a premixed section storing lyophilized non-specific reagents that do not correspond to the target, and six wells physically spaced apart from the premixed section. The number of wells can also be other than six, but six is ​​a preferred embodiment. At least one well in the six wells contains primer and probe reagents corresponding to no less than M targets, where M is an integer not less than 2. The primer and probe reagents are stored in a second dry state. It also includes a liquid-sealed reagent storage section containing paraffin oil. Figure 10 The illustration shows the elution transfer operation performed by the second-capacity elution pipetting consumable in conjunction with the pipetting module 50. The pipetting module 50 is connected to the elution pipetting consumable, and then moves to the elution well to draw up the elution according to step S3. Then, according to step S4, the elution is transferred twice to the premixing section of the amplification consumable. Similarly, the upper drive unit and the lower drive unit can drive the combined module and the stage 204 with overlapping time periods, thus enabling rapid and low-risk elution transfer. The 10-well connecting tube consumable has two types of pipetting consumable wells separated at both ends, minimizing the risk of cross-contamination caused by sample evaporation. This also ensures that the integrated instrument can obtain accurate and repeatable test results with minimal variation. During the transfer of eluent, a first volume of eluent is transferred, dissolving some of the lyophilized nonspecific reagent and increasing the free volume in the premixing section. Then, a second volume of eluent is transferred. The eluent pipetting consumable performs several aspiration and dispensing operations within the premixing section, ensuring thorough mixing of the eluent and nonspecific reagent without contamination, thus obtaining the premixed solution to be dispensed. This eliminates the need for different pipetting consumables for premixing and dispensing the premixed solution. Figure 11 a, Figure 11 As shown in b, the thoroughly mixed premixed solution is sequentially transferred to each dispensing cup well by the eluent pipetting consumable. Here, the premixed solution is dispensed using a multi-pipette / multi-dispense method, ensuring that the amount of premixed solution in each well is as consistent as possible, guaranteeing accurate dispensing. After the premixed solution is dispensed, as shown... Figure 11As shown in Figure c, the pipetting module uses a single aspiration and multiple dispensing method to transfer paraffin oil, less than the amount of premixed solution, from the liquid-sealed reagent storage unit to each well. The optimal volume of paraffin oil in each well is between 1 / 2 and 4 / 5 of the transferred premixed solution. This ensures that the amount of paraffin oil transferred is not excessive and does not affect the amplification reaction rate in the well, avoiding false negatives due to insufficient amplification. Furthermore, it ensures sufficient coverage of the premixed solution by the paraffin oil, achieving better liquid sealing and minimizing evaporation of the premixed solution, thus ensuring that the detection sensitivity and accuracy are not affected. Figure 11 As shown in d, the amplification consumables are finally closed to prepare for subsequent operations.

[0073] The expanded consumable switch cover mechanism 70 can adopt existing technical structural designs, such as the consumable switch cover structure disclosed in application number CN111847344A, which includes a transition plate, a consumable cover fixing plate, and a consumable body fixing plate; the transition plate, the consumable cover fixing plate, and the consumable body fixing plate are arranged sequentially from top to bottom; the transition plate and the consumable body fixing plate are movably sleeved on several vertically arranged guide rods and can move up and down along the guide rods; each guide rod has a guide rod first upper limit block at its upper end, which is located above the transition plate; each guide rod has a guide rod lower limit block at its lower end, which is located below the consumable body fixing plate; the transition plate is connected to the consumable cover fixing plate through several guide rods; the bottom of the consumable cover fixing plate is provided with a consumable cover fixing mechanism; and several consumable body fixing grooves are opened on the consumable body fixing plate.

[0074] Of course, the amplification consumable switch cover mechanism 70 can incorporate a hot cover function, thereby ensuring a high top temperature under the condition that the top of the amplification consumable is pressed tightly during the amplification process, thereby reducing or even avoiding problems such as condensation caused by encountering cold wall surfaces during the thermal cycle.

[0075] The sample tube consists of a container body and a container cap.

[0076] The sample tube opening and closing mechanism 20 can adopt existing technology structures, such as the rotary opening and closing mechanism disclosed in application number 202211017823.6, which includes a capping assembly for driving the container cap to rotate relative to the container body and to open or close it from the container body; a lifting assembly connected to the capping assembly for driving the capping assembly to complete at least a vertical movement; the capping assembly includes a rotating head with an external thread structure, which can engage with the internal thread of the container cap of the sample tube being opened and closed, and rotate counterclockwise or clockwise to drive the container cap to rotate counterclockwise or clockwise, thereby realizing the opening or closing of the container cap.

[0077] The combined module also includes an identification module 40. The upper drive unit can cooperate with the lower drive unit so that the first time period of movement of the identification module 40 at least partially overlaps with the second time period of movement of the corresponding support platform 204. The consumable identification camera of the identification module 40 can perform dynamic or static barcode scanning identification. The extraction consumables and amplification consumables are provided with identification codes that can be scanned by the identification module 40, such as barcodes, QR codes, RFID, etc., for functions such as object information acquisition and experimental database establishment.

[0078] The pipetting module 50 can transfer and discharge liquid at a first discharge speed and mix and discharge liquid at a second discharge speed. The first discharge speed is 1 / 3 of the second discharge speed. The speed of liquid aspiration and discharge of the pipetting module 50 can be controlled by adjusting the feed rate of the pipetting pusher. When the pipetting module 50 discharges liquid at the second discharge speed, the feed rate of the pipetting pusher is 10 mm / s.

[0079] The overlapping time period exceeds 50% of the shorter of the two time periods, thus enabling faster and more accurate transfer of sample solution.

[0080] The support platform 204 also includes a sample analysis module. Both can be driven simultaneously by the lower drive unit to achieve the same displacement. The sample analysis module includes a temperature circulation module and an optical detection module 310. The temperature circulation module includes an active heat dissipation unit to reduce temperature. The active heat dissipation unit includes a heat sink 307 and a bottom fan assembly. The heat sink 307 is connected to the automated loading area 303. The bottom fan assembly includes a bottom fan 308 and an exhaust channel 309. The bottom fan 308 and the exhaust channel 309 are connected, and the heat sink 307 is matched with the exhaust channel 309. The bottom fan 308 can force heat dissipation of the heat sink 307, enabling the integrated machine to dissipate heat more quickly and ensuring the stability of the internal system.

[0081] The support platform 204 can approach or move away from the first vent 801 under the action of the lower drive unit. For example, during the sample extraction and purification operation, there is a certain gap between the support platform and the first vent 801. During the cyclic amplification operation, the exhaust channel 309 is connected to the first vent 801, thus forming a relatively sealed first air duct. In this way, the waste heat generated by the amplification thermal cycle during the cyclic amplification operation will be quickly discharged outside the device housing 301 with minimal interference to the internal components of the integrated machine, so as not to leak into the housing 301 during the discharge process and cause waste heat accumulation that affects the circuit and other working environments, thus ensuring the reliability of the entire system. At the same time, the sample extraction and purification and cyclic amplification can be operated at different positions through the drive of the lower drive unit, thereby maximizing the reliability of the composite function operation on the integrated support platform and minimizing or even avoiding pollution caused by aerosols.

[0082] A second vent 802 is also provided on the same side of the housing 301 as the first vent 801. It can be directly or indirectly connected to an independent air duct 8021. More preferably, a top fan 8022 connected to the independent air duct 8021 can be provided. In this way, during the time interval between at least part of the support platform and the first vent 801 (for example, during the sample extraction and purification operation time after the extraction consumables are opened), the second vent 802 and the connected independent air duct 8021 are in operation to exhaust air from the integrated machine. This ensures that no serious aerosol pollution will be generated even when the sample extraction and purification operation is completed with the lid open. During the cyclic amplification operation, the top fan 8022 is always in operation. The air through the two air ducts can minimize the risk of mixing and cross-contamination.

[0083] A third ventilation opening 803 is also provided on the same side as the first ventilation opening 801. A central fan 8031 ​​is provided at the third ventilation opening 803, which can provide functions such as heat dissipation, negative pressure and pollution reduction during other time periods. A second air duct is provided between the third ventilation opening 803 and the bottom fourth ventilation opening 804, through which the air flows from the third ventilation opening 803 to the fourth ventilation opening 804.

[0084] Other time periods can be used for sample extraction and purification operations. Since the entire operation is carried out with the extraction consumables open, the potential risk of contamination is relatively high. During this time, the second air duct, separate from the support platform 204, can minimize the risk of cross-contamination caused by the similarity in airflow direction across most of the duct area. During this time period, because the first vent 801 is separated from the support platform 204, the first vent 801 can also serve as an auxiliary vent. A smaller amount of air enters the detection equipment housing 301 through the first vent 801 under the negative pressure generated by the internal airflow, thereby increasing the overall air exchange rate within the equipment housing 301. Figure 15Area A can be the extraction consumable area 305 on the support platform 204, and area B can be the amplification consumable area 306. Under these airflow conditions, the extraction consumable area 305 is located downwind of the amplification consumable area 306. Although the extraction consumables need to be operated with the lid open during the extraction and purification period, the extraction consumable area 305 is downwind, so even if there is a risk of contamination, it will not affect the detection results of the upwind amplification consumable area 306. Therefore, the final results obtained within the entire detection device are more accurate and reliable. Under negative pressure, a fourth airflow can be formed between the first vent 801 and the fourth vent 804 during this period. The overall airflow direction within the fourth airflow is from the first vent 801 to the fourth vent 804. Alternatively, to achieve the same effect, the fan can be placed on the fourth vent 804 located at the bottom of the housing 301.

[0085] Other time periods can also be cyclical expansion periods. The lower drive unit drives the support platform 204 to connect with the first vent 801. At this time, the waste heat generated during the thermal circulation process under the action of the bottom fan 308 can be quickly discharged outside the housing 301 through the relatively closed first air duct formed by the contact. The air carrying waste heat in this air duct can minimize the impact on the internal environment of the detection equipment, which is of great significance for the reliable operation of the circuit control devices. At the same time, by establishing a third air duct with the air flow direction from the fourth vent 804 to the third vent 803, the waste heat generated by circuit components can be quickly discharged, and the risk of internal contamination can be reduced. Meanwhile, the first air duct and the third air duct have basically the same outflow direction, which can reduce the risk of cross-contamination caused by turbulence and turbulence due to large differences in flow direction. Of course, the fourth vent 804 can also be located at the top of the housing 301, and the effect is similar to that at the bottom, which will not be repeated here. Of course, HEPA filters or other filter components can be installed in all or some of the above-mentioned vents to filter the incoming or outgoing air, thereby ensuring the safe and reliable operation of the instrument. The fans in different vents can be designed according to parameters such as flow rate, and the specific number is not limited.

[0086] The optical detection module 310 can sequentially detect the fluorescence signals of multiple fluorescence channels at multiple cup positions. The optical detection module 310 is located at the lower part of the exhaust channel 309 and is optically connected to the bottom of the amplification consumable area 306. Because the optical detection module 310 is positioned at the lower part of the exhaust channel 309, the hot air discharged from the exhaust channel 309 will not have a thermal impact on the optical detection module 310, ensuring the reliability of the system. This also allows the entire optical detection module 310 to be combined with the moving automated loading area 303 to achieve a relatively stationary position for both.

[0087] Example 2

[0088] A control method for a highly efficient mobile sample detection all-in-one machine, such as... Figure 1-17 As shown, the efficient mobile sample detection all-in-one machine described in Example 1 includes the following steps: machine operation steps: power on, top fan 8022 starts running.

[0089] Loading configuration steps: Open the opening and closing part 302. The first motor 101 and the second motor 201 in the lower drive unit work in series to drive the automated loading area 303 outside the housing 301. Use a barcode scanner to scan the sample tube to obtain the corresponding information of the sample tube. Load the sample tube in the sample tube area 304, load the extraction consumables in the extraction consumables area 305, and load the amplification consumables in the amplification consumables area 306. After loading is completed, the first motor 101 and the second motor 201 work in series to drive the automated loading area 303 back into the housing 301, and close the opening and closing part 302.

[0090] The scanning and recognition steps are as follows: the upper drive unit drives the recognition module 40 to move during the first time period, and the lower drive unit drives the carrier platform 204 to move during the second time period. The first time period and the second time period overlap at least partially. The recognition module 40 performs scanning and recognition on the extracted consumables and the amplified consumables respectively. When the upper drive unit and the lower drive unit drive simultaneously, the consumable recognition camera of the recognition module 40 performs dynamic scanning. When the upper drive unit drives alone, the consumable recognition camera of the recognition module 40 performs static scanning.

[0091] Sample tube opening procedure: The lifting component operates, causing the sample tube opening and closing mechanism 20 to descend to the position corresponding to the target sample tube. The capping component rotates counterclockwise, and the rotating head screws into the container cap. The capping component continues to rotate counterclockwise, causing the container cap to rotate counterclockwise, thus opening the container cap. The capping component then rises.

[0092] Steps for opening the cover to extract consumables: Open the cover opening mechanism 30 to extract consumables.

[0093] Sample transfer steps: The upper drive unit drives the pipetting module 50 to move during the first time period, and the lower drive unit drives the stage 204 to move during the second time period. The first and second time periods overlap at least partially. The upper and lower drive units cooperate to drive the pipetting module 50 to move between the sample tube and the lysis well of the extraction consumable to perform the sample transfer operation. Sample tube capping steps: The lifting component operates, causing the sample tube capping mechanism 20 to descend to the position corresponding to the target sample tube. The capping component rotates clockwise, causing the container cap to rotate clockwise, tightening the container cap onto the container body. The capping component continues to rotate clockwise, disengaging the rotating head from the container cap, and the capping component rises.

[0094] Sample extraction and purification steps: The upper drive unit drives the extraction module 60 to move during the first time period, and the lower drive unit drives the stage 204 to move during the second time period. The first and second time periods overlap at least partially. The upper and lower drive units cooperate with each other to drive the extraction module 60 to move between the wells of the extraction consumables to perform the sample extraction and purification operation.

[0095] Steps for opening the amplification consumables: Open the amplification consumables using the opening and closing mechanism 70.

[0096] The amplification system setup steps are as follows: The upper drive unit drives the pipetting module 50 to move during the first time period, and the lower drive unit drives the stage 204 to move during the second time period. The first and second time periods at least partially overlap. The upper and lower drive units cooperate to drive the pipetting module 50 to move between the elution wells of the extraction consumables and the premixing wells of the amplification consumables to perform elution buffer transfer operations. The pipetting module 50 performs n mixing operations in the premixing wells, where n is a positive integer. The mixing operation involves the pipetting module 50 aspirating the elution buffer from the elution wells and then discharging it into the premixing wells. After the elution buffer is mixed with the lyophilized reagent, the upper and lower drive units cooperate to drive the pipetting module 50 to move between the wells of the amplification consumables to perform solution dispensing operations. The liquid module 50 uses a multi-absorption, multi-displacement method to sequentially transfer the thoroughly mixed premixed solution into the well positions of the aliquot cups. This precise multi-absorption, multi-displacement scheme ensures a more consistent amount of premixed solution in each well position. After the premixed solution is distributed, a smaller amount of paraffin oil than the amount of premixed solution transferred is drawn from the liquid-sealed reagent storage section using a single aspiration, multi-displacement method and transferred to each well position. The optimal volume of paraffin oil in each well position is between 1 / 2 and 4 / 5 of the transferred premixed solution. This ensures that the amount of paraffin oil transferred is not excessive, so as not to affect the amplification reaction rate in the well position and avoid false negatives due to insufficient amplification. On the other hand, it also ensures that the paraffin oil fully covers the premixed solution, achieving better liquid sealing and minimizing the evaporation of the reaction solution, thus ensuring that the detection sensitivity and accuracy are not affected.

[0097] Steps for removing consumables and closing the cover: The consumables removal cover opening and closing mechanism 30 closes the consumables removal process.

[0098] Amplification consumables closing procedure: The amplification consumables opening and closing mechanism 70 closes the amplification consumables.

[0099] Cyclic amplification steps: The intermediate fan runs, the temperature cycling module is activated, and PCR thermal cycling amplification is performed.

[0100] Analysis steps: Output sample fragment analysis results based on fluorescence analysis.

[0101] In the amplification system setup step, the pipetting module 50 transfers the eluent from the elution well of the extraction consumable to the premixing well of the amplification consumable in two stages. The first stage involves the pipetting module 50 transferring a first volume of eluent into the premixing well to dissolve the lyophilized reagent and ensure sufficient space within the well. The second stage involves the pipetting module 50 transferring a second volume of eluent into the premixing well, where the first volume of eluent is 0.2-0.9 times the volume of the second volume. This two-stage transfer of the eluent prevents overflow due to the lyophilized reagent not dissolving immediately.

[0102] In the amplification system setup step, the pipetting module 50 transfers and discharges liquid at a first discharge rate during pipetting and mixes and discharges liquid at a second discharge rate during mixing. The first discharge rate is 1 / 4 to 1 / 2 of the second discharge rate. This achieves more thorough mixing without generating microbubbles that could affect the test results, and also prevents the problem of excessively fast discharge during pipetting leading to severe wall adhesion and potentially false negative test results.

[0103] During the cyclic amplification step, the lower drive unit drives the support platform 204 to move towards the first vent 801 until the exhaust channel 309 connects with the first vent 801, forming a relatively sealed first air duct. The heat sink 307 and the bottom fan 308 are activated to cool the amplification consumable area. At the same time, the middle fan 8031 ​​is activated to establish a third air duct with the airflow direction from the fourth vent 804 to the third vent 803.

[0104] In the analytical steps, the principle of fluorescence analysis is as disclosed in the multi-fluorescence channel detection system for real-time quantitative PCR disclosed in application number CN201610152466.2. This system includes a fluorescence detection unit, an optical fiber disk, and a rotating disk. The fluorescence detection unit includes a light source, an excitation filter, a dichroic mirror, an optical fiber coupling lens, an optical fiber, a detection filter, and a photoelectric sensor. The dichroic mirror integrates the existing excitation and detection units into a single unit. Light emitted from the light source is filtered sequentially by the excitation filter, coupled by the optical fiber coupling lens, and finally incident into the test tube through the optical fiber to excite the fluorescent substance in the sample, generating fluorescence. A portion of the fluorescence returns sequentially from the optical fiber to the optical fiber coupling lens for collimation, and is filtered out by the detection filter to obtain pure fluorescence. Finally, the fluorescence is incident on the photoelectric sensor for photoelectric conversion. Multiple optical fibers are inserted into the optical fiber disk, and multiple fluorescence detection units are distributed on the rotating disk. The rotating disk rotates once around the center of the optical fiber disk, allowing for the sequential detection of fluorescence signals from multiple fluorescent channels in multiple test tube wells.

[0105] Figure 17The diagram illustrates a control module for the all-in-one machine of this invention. To achieve low-cost and high-efficiency control of the all-in-one machine, the drive module is divided into three sub-drive modules: sub-drive module one, sub-drive module two, and sub-drive module three, as shown in the diagram. Each sub-drive module can communicate with the core board via a CAN bus. Each sub-drive module can communicate with the core board using any publicly available query-response mechanism to obtain control commands converted by the core board's conversion board. These control commands can be transmitted in the form of functions or tables, etc., without limitation. The core board can be connected to a switch and an industrial control computer via a LAN connection. The industrial control computer can be connected to a PC via a LAN connection for remote control and can also be connected to a touch screen to receive and edit touch screen information. Of course, multiple industrial control computers can be connected in parallel via wired or wireless means, or connected via USB for version upgrades, etc. Each sub-drive module can control the operation of different driven units according to the control commands transmitted by the core board. Optimally, the upper drive unit of the drive assembly module and the lower drive unit of the drive platform are configured in two different sub-drive modules. This allows the two sub-drive modules to execute the control commands transmitted by the core board in parallel, and to simultaneously output the drive commands of the upper and lower drive units during overlapping time periods. Furthermore, there is no complex control coordination requiring a clock circuit. In this configuration, the two sub-drive modules only need to independently arrange the drive timing according to their respective internal clock circuits based on the control commands, making the control more efficient. This invention also includes multiple displacement sensors, which can perform calibration of different mechanisms and provide accurate references for precise displacement control. They can also collaboratively determine whether the integrated machine correctly executes the control commands issued by the core board and provide timely feedback on the final control state.

[0106] Table 1 shows the results of eight reproducible multiplex assays performed using the integrated instrument of this invention. The results in Table 1 demonstrate that the integrated instrument of this invention, by employing a combination of upper and lower drive units, reduces consumable exposure time and achieves efficient and stable multiplex assay results. Furthermore, the unique arrangement of consumable well positions reduces the possibility of cross-paths in sample transfer, extraction, and elution buffer transfer, further contributing to efficient and stable assay results. The results obtained from eight assays using this integrated instrument for different targets showed minimal deviation. Low STD and low CV values ​​indicate a reasonable design layout for the integrated instrument, reducing the impact of contamination on experimental results and demonstrating high reproducibility.

[0107] Table 1. Results of Repeatability Verification of Multiple Detection Samples Performed by the All-in-One Machine of the Present Invention

[0108]

[0109] This article uses specific embodiments to illustrate the principles and implementation methods of the present invention. The descriptions of the embodiments above are only for the purpose of helping to understand the method and core ideas of the present invention. It should be noted that those skilled in the art can make several improvements and modifications to the present invention without departing from the principles of the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

[0110] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of this invention is usually placed during use. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0111] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

Claims

1. A high-efficiency mobile sample detection all-in-one machine, characterized in that, The device comprises an upper region and a lower region; the upper region comprises an upper driving unit and a combined module, the upper driving unit can drive the combined module to move horizontally, and the combined module comprises a pipetting module and an extraction module; the lower region comprises a lower driving unit and a bearing table, the bearing table comprises a sample tube area, an extraction consumable area and an amplification consumable area, and the lower driving unit can drive the bearing table to move horizontally; the time period for the upper driving unit to drive the combined module to move is a first time period, the time period for the lower driving unit to drive the bearing table to move is a second time period, there is an overlapping time period between the first time period and the second time period, the upper driving unit and the lower driving unit cooperate with each other, so that the pipetting module can move between the sample tube area and the extraction consumable area to perform a sample liquid transfer operation, the extraction module can move in the extraction consumable to perform a sample extraction and purification operation, the pipetting module can move between the extraction consumable area and the amplification consumable area to perform an eluent transfer operation, and the pipetting module can move in the amplification consumable area to perform a solution dispensing operation; the upper driving unit drives the combined module at a first precision, the combined module moves horizontally at a first speed under the action of the upper driving unit, the lower driving unit drives the bearing table to move horizontally at a second precision, the bearing table moves horizontally at a second speed under the action of the lower driving unit, the first precision is higher than the second precision and the first speed is lower than the second speed, or the first precision is lower than the second precision and the first speed is higher than the second speed.

2. The all-in-one machine for efficient mobile sample testing of claim 1, wherein, The lower driving unit comprises a first motor and a second motor, the first motor can drive the bearing table to move within a first movement distance, the second motor can drive the bearing table to move within a second movement distance, and the first motor and the second motor cooperate with each other to make the movement distance of the bearing table not exceed the sum of the first movement distance and the second movement distance; the first motor and the second motor can cooperate with each other to drive the bearing table to perform a loading configuration operation outside the housing; and the first motor and the second motor work in series.

3. The all-in-one machine for efficient mobile sample testing of claim 1, wherein, The combined module further comprises an identification module, the upper driving unit can cooperate with the lower driving unit to make the first time period corresponding to the movement of the identification module at least partially overlap with the second time period corresponding to the movement of the bearing table; and the consumable identification camera of the identification module can perform dynamic code scanning identification or static code scanning identification.

4. The all-in-one machine for efficient mobile sample testing of claim 1, wherein, The pipetting module performs transfer liquid discharge at a first liquid discharge speed and performs mixing liquid discharge at a second liquid discharge speed, and the first liquid discharge speed is 1 / 4-1 / 2 of the second liquid discharge speed.

5. A control method of the high-efficiency mobile sample detection all-in-one machine according to any one of claims 1 to 4, characterized in that, The device comprises the following steps, a sample liquid transfer step: the upper driving unit drives the pipetting module to move within a first time period, the lower driving unit drives the bearing table to move within a second time period, the first time period and the second time period at least partially overlap, and the upper driving unit and the lower driving unit cooperate with each other to drive the pipetting module to move between the sample tube and the lysis hole of the extraction consumable to perform a sample liquid transfer operation; The sample extraction and purification step includes: the upper driving unit drives the extraction module to move in a first time period, the lower driving unit drives the carrying table to move in a second time period, the first time period and the second time period at least partially overlap, and the upper driving unit and the lower driving unit cooperate to drive the extraction module to move between the hole positions of the extraction consumables to perform the sample extraction and purification operation. The amplification system establishment step includes: the upper driving unit drives the pipetting module to move in a first time period, the lower driving unit drives the carrying table to move in a second time period, the first time period and the second time period at least partially overlap, and the upper driving unit and the lower driving unit cooperate to drive the pipetting module to move between the elution hole positions of the extraction consumables and the premix hole positions of the amplification consumables to perform the eluent transfer operation, the pipetting module performs n times of mixing operation in the premix hole positions, n is a positive integer, the mixing operation is that the pipetting module sucks the eluent in the elution hole position and then discharges the eluent into the premix hole position, after the eluent is mixed with the freeze-dried reagent, the upper driving unit and the lower driving unit cooperate to drive the pipetting module to move between the hole positions of the amplification consumables to perform the solution dispensing operation.

6. The control method of the all-in-one machine for efficient mobile sample testing according to claim 5, characterized in that, In the amplification system establishment step, the pipetting module transfers the eluent from the elution hole positions of the extraction consumables to the premix hole positions of the amplification consumables for m times, m is an integer greater than or equal to 2.

7. The control method of the all-in-one machine for efficient mobile sample testing according to claim 5, characterized in that, In the amplification system establishment step, the pipetting module transfers the eluent at a first discharge speed in the pipetting process and mixes the eluent at a second discharge speed in the mixing operation, the first discharge speed is 1 / 4-1 / 2 of the second discharge speed.

8. The control method of the all-in-one machine for efficient mobile sample testing according to claim 5, wherein, Before the sample liquid transfer step, the method further includes The loading configuration step includes: the first motor and the second motor in the lower driving unit work in series to drive the carrying table outside the shell, load the sample tubes in the sample tube area, load the extraction consumables in the extraction consumables area, and load the amplification consumables in the amplification consumables area, after the loading is completed, the first motor and the second motor work in series to drive the carrying table to return to the shell.

9. The control method of the all-in-one machine for efficient mobile sample testing according to claim 8, characterized in that, After the loading configuration step, the method further includes The code scanning and identifying step includes: the upper driving unit drives the identification module to move in a first time period, the lower driving unit drives the carrying table to move in a second time period, the first time period and the second time period at least partially overlap, and the identification module respectively scans and identifies the extraction consumables and the amplification consumables, wherein, when the upper driving unit and the lower driving unit drive at the same time, the consumable identification camera of the identification module performs dynamic code scanning; when the upper driving unit drives alone, the consumable identification camera of the identification module performs static code scanning.

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