A silicon-based chip-based built-in reagent detection card box

Abstract

The application belongs to the technical field of nucleic acid detection, and specifically discloses a built-in reagent detection card box based on a silicon-based chip, which comprises a reaction chip assembly and a sample tube assembly that can be assembled together; the reaction chip assembly comprises a silicon-based chip, a chip seat, a sealing block and a sample injection needle; the sample tube assembly comprises a sample storage tube and a liquid pressing piece; the silicon-based chip, the chip seat, the sample injection needle and the sample storage tube are sequentially connected. The built-in reagent detection card box disclosed by the application pre-buries freeze-dried reagents in a sample injection groove of the chip seat and pre-buries sample storage liquid in the sample storage tube; after sampling on site, a user can independently mix the sample with the sample storage liquid and the freeze-dried reagents, extract the sample and prepare the reagents, and then detect in a matched instrument, thereby reducing the dependence on a laboratory and a professional technician and obtaining a detection result on site.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of nucleic acid detection, and particularly relates to a built-in reagent detection card box based on a silicon-based chip. BACKGROUND

[0002] Nucleic acid detection is one of main detection means in medicine and diagnostics. When detecting, a sample is first collected and stored in a sampling tube, and then is sent to a laboratory to be extracted, purified and detected by a professional technician using a large instrument. The whole detection process includes the steps of lysis, extraction, amplification and detection. Specifically, the sample is added to a sample preservative, and then is extracted by an extraction instrument (or manually extracted) to obtain nucleic acid in the sample and remove interfering substances in the sample. Then, the sample nucleic acid liquid is added to a detection reagent to be fully mixed, and then amplification and detection are started. The whole process takes a long time, has high requirements for sample transportation, high transportation cost, high transportation risk, high requirements for detection sites, needs to be performed in a special PCR laboratory, and has high dependence on professional technicians.

[0003] A commonly used antigen detection (colloidal gold test strip method) adds the collected sample to a sample preservative, drops the preservative on a test strip after the sample is fully released, and detects. The operation is simple. However, this method can only be used for antigen-antibody detection, cannot be used for nucleic acid detection, and has low detection sensitivity.

[0004] Therefore, a portable nucleic acid detection device that reduces dependence on laboratories and professional technicians and can be detected on site in real time is needed. SUMMARY

[0005] The application aims to provide a built-in reagent detection card box based on a silicon-based chip to solve the above technical problems.

[0006] The technical scheme provided by the application is as follows: a built-in reagent detection card box based on a silicon-based chip, which comprises a reaction chip assembly and a sample tube assembly that can be assembled together; the reaction chip assembly comprises a silicon-based chip, a chip seat, a sealing block and a sample injection needle; the sample tube assembly comprises a sample storage tube and a liquid pressing member; the silicon-based chip, the chip seat, the sample injection needle and the sample storage tube are connected in sequence; a plurality of openings are formed in the silicon-based chip, the number of the openings is even, and adjacent two openings form a group, one of which is a sample injection port and the other is an exhaust port; the sample injection needle is provided with an exhaust hole; the sealing block is arranged in the exhaust hole; the chip seat is provided with an insertion slot, a sample injection slot and a sample outlet slot; the insertion slot is used for connecting the silicon-based chip and the chip seat, the sample injection slot is pre-installed with a freeze-dried reagent, the sample injection slot is in communication with the sample injection port of the silicon-based chip and the needle hole of the sample injection needle, and the sample outlet slot is in communication with the exhaust port of the silicon-based chip and the exhaust hole; the sample storage tube is pre-installed with a sample storage liquid, the sample storage tube is provided with a first sample injection hole, the first sample injection hole is used for inserting the sample injection needle, and the liquid pressing member is used for pushing the liquid in the sample storage tube into the silicon-based chip; wherein, after the sample is collected in the sample storage tube, the sample injection needle is inserted into the sample storage tube to connect the sample injection needle and the sample storage tube.

[0007] Preferably, a sealing strip is arranged between the silicon-based chip and the chip seat, the sealing strip is provided with a connecting hole, and each connecting hole is provided with a sealing edge extending outward and matched with the opening of the silicon-based chip.

[0008] Preferably, the size of the sealing edge is 0-0.1 mm larger than that of the sealing groove.

[0009] Preferably, a needle seat sealing pad is arranged between the chip seat and the sample injection needle, and the sample injection slot and the sample outlet slot are provided with a sealing groove; the needle seat sealing pad is provided with a sealing edge extending outward and matched with the sealing groove.

[0010] Preferably, the sample storage tube is further provided with a second sample injection hole for the sample to enter, the number of the second sample injection hole is one or more, and the plurality of second sample injection holes are in communication or not in communication.

[0011] Preferably, the application further comprises a tube cover capable of closing the second sample injection hole.

[0012] Preferably, the liquid pressing member comprises a liquid pressing plug, and the liquid pressing plug is in interference sealing tight fit connection with the inner wall of the sample storage tube.

[0013] Preferably, the liquid pressing member comprises a push valve, the tube cover is provided with a through hole, one end of the push valve passes through the through hole and is buckled with the liquid pressing plug, the push valve is provided with a handle and a limiting protrusion capable of passing through the through hole, the handle is located outside the tube cover, and the size of the handle and the limiting protrusion is greater than that of the through hole.

[0014] Preferably, the sample injection needle is sleeved with a rubber tube.

[0015] Preferably, the sealing block is a self-sealing block.

[0016] The application has the advantages that:

[0017] 1. The built-in reagent detection card provided by the application is based on a silicon chip, and a part of the card is a reaction chip assembly, which comprises a silicon chip and a chip seat with pre-embedded freeze-dried reagents, and the freeze-dried reagents are pre-embedded in a sample inlet groove of the chip seat; another part of the card is a sample tube assembly, which comprises a sample storage tube with pre-embedded sample storage liquid, and the sample storage liquid is used for extracting nucleic acids in a sample and diluting interfering substances in the sample. The chip seat, a sample inlet needle, the sample storage tube and a tube cover are sequentially buckled and connected, so that the built-in reagent detection card can be quickly and easily assembled. After the collected sample is fully released in the sample storage tube, the sample inlet needle with a rubber sleeve is inserted into the sample storage tube, so that the chip seat and the sample inlet needle are connected to form a closed space, and the built-in reagent detection card is loaded into an instrument for sample detection. In the whole process, after the sample is collected on site by using the built-in reagent detection card, the sample can be mixed with the sample storage liquid and the freeze-dried reagents by the user, the sample is extracted and the reagent is prepared, and then the sample is detected in a matching instrument, so that the dependence on a laboratory and a professional technician is reduced, and the detection result can be obtained on site.

[0018] 2. The built-in reagent detection card provided by the application is based on a silicon chip, and a part of the card is a reaction chip assembly, which comprises a silicon chip and a chip seat with pre-embedded freeze-dried reagents, and the freeze-dried reagents are pre-embedded in a sample inlet groove of the chip seat; another part of the card is a sample tube assembly, which comprises a sample storage tube with pre-embedded sample storage liquid, and the sample storage liquid is used for extracting nucleic acids in a sample and diluting interfering substances in the sample. The chip seat, a sample inlet needle, the sample storage tube and a tube cover are sequentially buckled and connected, so that the built-in reagent detection card can be quickly and easily assembled. After the collected sample is fully released in the sample storage tube, the sample inlet needle with a rubber sleeve is inserted into the sample storage tube, so that the chip seat and the sample inlet needle are connected to form a closed space, and the built-in reagent detection card is loaded into an instrument for sample detection. In the whole process, after the sample is collected on site by using the built-in reagent detection card, the sample can be mixed with the sample storage liquid and the freeze-dried reagents by the user, the sample is extracted and the reagent is prepared, and then the sample is detected in a matching instrument, so that the dependence on a laboratory and a professional technician is reduced, and the detection result can be obtained on site.

[0019] 3. In the built-in reagent detection card provided by the application, a sealing strip is arranged between the silicon chip and the chip seat, a needle seat sealing pad is arranged between the chip seat and the sample inlet needle, sealing grooves are arranged on the sample inlet groove and the sample outlet groove, and a sealing edge extending outward and matched with the sealing grooves is arranged on the needle seat sealing pad. The arrangement of the sealing strip, the needle seat sealing pad, the sealing grooves and the sealing edge effectively ensures the stable sealing of the overall structure, so that the small open sample inlet groove is more easily sealed.

[0020] 4. In the built-in reagent detection card provided by the application, a sealing block is arranged in the exhaust hole. When the sealing block is self-sealing, it can be quickly self-sealed after encountering liquid, so as to achieve the effect of sealing the liquid as a whole.

[0021] 5. In the built-in reagent detection card provided by the application, all operations after sample addition are in a closed state, liquid transfer is not required after opening the cover, pollution can be avoided, on-site detection is more suitable, and the dependence on a laboratory environment is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 Exploded view of the silicon-based chip-based built-in reagent detection cartridge of Example 1;

[0023] Figure 2 Longitudinal sectional view of the reaction chip assembly in Example 1;

[0024] Figure 3 Perspective view of the sealing strip in Example 1;

[0025] Figure 4 Perspective view of the silicon-based chip in Example 1;

[0026] Figure 5 Perspective view of the needle seat sealing pad in Example 1;

[0027] Figure 6 Longitudinal sectional view of the sample tube assembly in Example 1;

[0028] Figure 7 Longitudinal sectional view of the reaction chip assembly and the sample tube assembly connected in Example 1;

[0029] Figure 8 Longitudinal sectional view of the sample tube assembly in Example 2;

[0030] Figure 9 Longitudinal sectional view of the sample tube assembly in Example 3;

[0031] Figure 10 Longitudinal sectional view of the sample tube assembly in Example 4;

[0032] Figure 11 Longitudinal sectional view of the sample tube assembly in Example 5.

[0033] Reference signs: silicon-based chip 1, sealing strip 2, chip seat 3, insertion slot 301, sample inlet slot 302, sample outlet slot 303, lyophilized reagent 4, needle seat sealing pad 5, sealing block 6, sample inlet needle 7, air vent hole 701, rubber tube 8, reaction tube sealing film 9, sample storage tube 10, first sample inlet hole 101, second sample inlet hole 102, clamping block 103, push valve 11, limiting protrusion 1101, inner tube sealing film 12, outer tube sealing film 13, liquid pressing plug 14, tube cover 15, sealing rib 16, reaction chip assembly 17, sample tube assembly 18, partition plate 19, screw rod 20, liquid injection nut 21, screw cap 22, pressing plate 23, pressing rod 24, pressing sheet 25. DETAILED DESCRIPTION

[0034] The following is further described in detail through specific embodiments:

[0035] Example 1

[0036] As Figures 1-7As shown, this embodiment provides a built-in reagent detection cartridge based on a silicon-based chip, including a reaction chip assembly 17 and a sample tube assembly 18.

[0037] like Figures 1 to 5 As shown, the reaction chip assembly 17 includes a silicon-based chip 1, a sealing strip 2, a chip holder 3, a needle holder sealing gasket 5, a sealing block 6, an injection needle 7, a glue cartridge 8, and a reaction tube sealing film 9. The silicon-based chip 1 is made of silicon and transparent heat-resistant materials, such as glass, polycarbonate (PC), or polypropylene (PP). Figure 1 , Figure 6 and Figure 7 As shown, the sample tube assembly 18 includes a sample storage tube 10, a liquid-pressing component, an inner sealing film 12, an outer sealing film 13, and a cap 15. The liquid-pressing component is used to push the liquid in the sample storage tube 10 into the silicon-based chip 1. The liquid-pressing component includes a push valve 11 and a liquid-pressing plug 14.

[0038] The reaction chip assembly 17 and the sample tube assembly 18 are assembled together via the injection needle 7 and the sample preservation tube 10. After assembly, the silicon-based chip 1, chip holder 3, injection needle 7, and sample preservation tube 10 are connected in sequence. Specifically, the chip holder 3 and the injection needle 7 are connected as one unit by a snap-fit. In this embodiment, the chip holder 3 has a slot on its side, and the injection needle 7 has a snap-fit ​​that matches the slot. After the sample to be collected is fully released in the sample preservation tube 10, the injection needle 7 inserts into the sample preservation tube 10, connecting the injection needle 7 and the sample preservation tube 10, thereby assembling the reaction chip assembly 17 and the sample tube assembly 18 together, and then loading them into the instrument for sample detection.

[0039] (1) Regarding the reaction chip assembly 17

[0040] When the reaction chip assembly 17 and the sample tube assembly 18 are not connected (when the injection needle 7 is not inserted into the sample storage tube 10), the injection needle 7 is covered with a reaction tube sealing film 9.

[0041] The silicon-based chip 1 has a reaction chamber inside.

[0042] A rubber sleeve 8 is attached to the outer sleeve of the injection needle 7. The injection needle 7 has a vent 701, and a sealing block 6 is installed inside the vent 701 to achieve sealing after the reaction chamber of the silicon-based chip 1 is filled. The sealing block 6 is a smooth-surfaced, tightly fitted, slidable rubber plug or a self-sealing knot. In this embodiment, the sealing block 6 is a self-sealing knot. When not in contact with liquid, the self-sealing knot acts as a vent; upon contact with liquid, it quickly self-seales, thus achieving a sealing effect. The vent 701 and the self-sealing knot help control the liquid volume inside the chip 1 while ensuring the reaction system is sealed and does not cause contamination.

[0043] In other alternative embodiments, the sealing block 6 is a rubber plug that is sealed and slidably connected within the vent 701.

[0044] The needle head of the sample injection needle 7 is provided with a rubber sleeve 8, which plays a sealing role when the sample injection needle 7 is connected to the sample storage tube 10.

[0045] The chip seat 3 is provided with an insertion slot 301 for inserting the silicon-based chip 1. The silicon-based chip 1 is fixedly connected to the chip seat 3 through the insertion slot 301. In this embodiment, the silicon-based chip 1 is tightly fitted with the chip seat 3 and is fixedly connected to the chip seat 3 as a whole through glue (epoxy resin or ultraviolet glue, etc.). In order to ensure the sealing, the insertion slot 301 is provided with a sealing strip 2, and the material of the sealing strip 2 is a material with elasticity and good biological compatibility such as silica gel or rubber.

[0046] The silicon-based chip 1 is provided with a plurality of openings, the number of the openings is even, and adjacent two openings form a group, one of which is a sample inlet and the other of which is an exhaust port.

[0047] The sealing strip 2 is provided with a connecting hole corresponding to the opening, and each connecting hole is provided with a sealing rib 16 extending outward, thereby achieving better sealing effect with the silicon-based chip 1.

[0048] The chip seat 3 is provided with a sample injection groove 302 and a sample outlet groove 303. After the silicon-based chip 1 is inserted into the insertion slot 301, the sample injection groove 302, the sample inlet of the silicon-based chip 1, the reaction chamber of the silicon-based chip 1, the exhaust port of the silicon-based chip 1 and the sample outlet groove 303 are sequentially communicated.

[0049] In order to ensure the sealing, a needle seat sealing gasket 5 is arranged between the chip seat 3 and the sample injection needle 7. The needle seat sealing gasket 5 is provided with a communication hole, and the needle seat sealing gasket 5 is provided with a sealing rib 16 extending outward and matching the sample injection groove 302 and the sample outlet groove 303.

[0050] The sample injection groove 302 is pre-installed with a lyophilized reagent 4 (the lyophilized reagent 4 can be in the form of particles), and the sample injection groove 302 is communicated with the sample inlet of the silicon-based chip 1 through the connecting hole of the sealing strip 2, and the sample injection groove 302 is communicated with the needle hole of the sample injection needle 7 through the communication hole of the needle seat sealing gasket 5. One end of the sample outlet groove 303 is communicated with the exhaust port of the silicon-based chip 1 through the connecting hole of the sealing strip 2, and the other end of the sample outlet groove 303 is communicated with the exhaust hole 701 of the sample injection needle 7 through the communication hole of the needle seat sealing gasket 5.

[0051] The sample injection groove 302 and the sample outlet groove 303 are provided with a sealing groove, and the needle seat sealing gasket 5 is provided with a sealing rib 16 matching the sealing groove, so as to achieve better sealing effect. The size of the sealing rib 16 is 0-0.1 mm larger than that of the sealing groove, the sealing rib 16 is made of soft silicone rubber, and the sealing groove is made of hard material such as plastic.

[0052] (2) About the sample tube assembly 18

[0053] The sample storage tube 10 is pre-filled with a sample storage solution for extracting nucleic acids from the sample and diluting interfering substances in the sample. Figure 6 In the embodiment, the sample storage tube 10 is provided with a first sample inlet hole 101 for insertion of the sample needle 7 and a second sample inlet hole 102 for sample entry. When the reaction chip assembly 17 and the sample tube assembly 18 are not connected, the first sample inlet hole 101 is attached with the tube inner sealing film 12 and the second sample inlet hole 102 is attached with the tube outer sealing film 13.

[0054] The tube cover 15 is used to seal the second sample inlet hole 102. In the embodiment, the tube cover 15 is integrally connected with the sample storage tube 10. By rotating the tube cover 15, the second sample inlet hole 102 of the sample storage tube 10 can be sealed or opened.

[0055] The sample needle 7, the sample storage tube 10 and the tube cover 15 are connected in sequence by buckling and are connected with the chip seat 3 and the sample needle 7 in the same or similar manner.

[0056] When the sample needle 7 and the sample storage tube 10 are assembled, the needle head of the sample needle 7 is inserted into the sample storage tube 10 through the first sample inlet hole 101. Before the sample needle 7 pierces the tube inner sealing film 12, the rubber cylinder 8 will first enter the first sample inlet hole 101 and be tightly fitted with the first sample inlet hole 101, and finally be clamped at the top end of the first sample inlet hole 101 to achieve sealing.

[0057] The liquid pressing plug 14 is tightly sealed and connected with the inner wall of the sample storage tube 10 in interference fit to prevent liquid leakage. The tube cover 15 is provided with a through hole with a positioning slot in the center. One end of the push valve 11 is inserted into the through hole and buckled with the liquid pressing plug 14. The push valve 11 is provided with a handle outside the tube cover 15. The handle is larger than the through hole. When the tube cover 15 is closed, the tube cover 15 is fixed with the sample storage tube 10 by buckling. The push valve 11 is also provided with a limiting protrusion 1101 which can pass through the through hole. The limiting protrusion 1101 is larger than the through hole. The limiting protrusion 1101 is used to limit the push valve 11. Only when the push valve 11 is subjected to a pushing force, the push valve 11 can move into the sample storage tube 10, thereby controlling the pushing of the sealing plug 14 to mix the sample with the sample storage solution. The limiting protrusion 1101 is larger than the through hole. After the push valve 11 is pushed into the sample storage tube 10, the push valve 11 cannot be restored to the initial position. In addition, by controlling the distance that the push valve 11 and the sealing plug 14 move into the sample storage tube 10, the volume of the sample injected into the silicon-based chip 1 can be controlled. Initially, the limiting protrusion 1101 is outside the tube cover 15. By pushing the handle downward, the limiting protrusion 1101 will pass through the through hole of the tube cover 15 and enter the inside of the tube cover 15. At the same time, the handle of the push valve 11 is blocked by the tube cover 15 and cannot be pressed further, thereby achieving the function of controlling the volume of the sample.

[0058] Along the center line direction of the sample storage tube 10, one or more second sample injection holes 102 are opened on the sample storage tube 10. Figure 6 ].

[0059] When the number of the second sample injection holes 102 is one, or the second sample injection holes 102 are connected, only one sample is detected.

[0060] When the second sample injection holes 102 are not connected, the number of the second sample injection holes 102 is consistent with the number of the reaction chambers on the silicon-based chip 1, the number of the sample injection grooves 302 and the sample outlet grooves 303 opened on the chip seat 3 matches the number of the second sample injection holes 102, and the simultaneous detection of multiple samples can be realized. The sample injection needle 7 and the liquid pressing plug 14 are arranged on each second sample injection hole 102. The freeze-dried reagent 4 in each sample injection groove 302 can be the same for detecting the same target or can be used for detecting different targets.

[0061] In this embodiment, the sample storage tube 10 is preferably provided with one second sample injection hole 102.

[0062] During detection, the user first takes out the sample tube assembly 18, tears the tube outer sealing film 13, adds the nasal swab or throat swab sample into the sample storage tube 10 through the second sample injection hole 102, rotates the tube cap 15, and buckles it on the sample storage tube 10 to mix the sample and the sample storage liquid to obtain a mixed liquid. The reaction chip assembly 17 is taken out, the reaction tube sealing film 9 at the needle opening of the sample injection needle 7 is torn, the needle opening of the sample injection needle 7 is inserted into the first sample injection hole 101 of the sample storage tube 10 to pierce the inner sealing film 12, the reaction chip assembly 17 and the sample tube assembly 18 are integrated through the buckling of the sample injection needle 7 and the sample storage tube 10. The handle of the push valve 11 is pressed to push the liquid pressing plug 14 into the sample storage tube 10, so that the mixed liquid enters the sample injection groove 302 through the sample injection needle 7, is first mixed with the freeze-dried reagent 4 pre-embedded in the sample injection groove 302, and then flows into the reaction chamber of the silicon-based chip 1 through another opening of the silicon-based chip 1. The excess gas in the silicon-based chip 1 is discharged through another opening of the silicon-based chip 1 and the exhaust hole 701 of the sample injection needle 7. The liquid continues to be added until the silicon-based chip 1 is filled, and the liquid flows to the sample outlet groove 303 and meets the sealing block 6 in the exhaust hole 701 of the sample injection needle 7. The sealing block 6 is self-sealing, which can be breathable under dry conditions, but sealed and unable to pass gas after meeting water, so as to realize the sealing of the silicon-based chip 1 and the chip seat 3. At this time, the limiting protrusion 1101 of the push valve 11 has been pressed into the tube cap 15, and the whole sample adding process is completed. Finally, the whole built-in reagent detection card box is placed in the matching instrument for detection, and the detection result is obtained on site.

[0063] Embodiment 2

[0064] The main difference between this embodiment and embodiment 1 is that Figure 8As shown, the tube cap 15 is fastened to the second inlet hole 102 of the sample storage tube 10 (the tube cap 15 has an installation groove that matches the end of the sample storage tube 10).

[0065] The pressure component is an arc-shaped, non-elastic pressure plate 25 located in the middle of the tube cap 15. In this embodiment, the tube cap 15 and the side of the pressure plate 25 are integrally connected. A snap-fit ​​block 103 is integrally connected to the inner wall of the sample storage tube 10, and a snap-fit ​​groove is formed in the snap-fit ​​block 103. The end of the pressure plate 25 is inserted into the snap-fit ​​groove. The snap-fit ​​block 103 and the snap-fit ​​groove help maintain the shape of the pressure plate 25 and position it.

[0066] Pressing the pressure plate 25 increases the air pressure inside the sample preservation tube 10, driving the sample and sample mixture to flow towards the silicon-based chip 1.

[0067] In Example 1, the liquid-pressing plug 14 is pressed into the second sample inlet 102 by flipping the tube cap 15. Compared with Example 1, in this example, the tube cap 15 is directly fastened to the second sample inlet 102 of the sample storage tube 10, which is more labor-saving and simpler. In addition, compared with Example 1, the liquid-pressing component in this example is a pressing plate 25 integrally connected to the tube cap 15, which drives the sample and sample mixture to flow to the silicon-based chip by air pressure, making the operation even simpler.

[0068] Example 3

[0069] The main difference between this embodiment and Embodiment 1 is that: Figure 9 As shown, the limiting protrusion 1101 is located at the end of the handle of the push valve 11, making the machining process simpler.

[0070] Example 4

[0071] The main difference between this embodiment and Embodiment 1 is that: Figure 10 As shown, the tube cap 15 has a liquid-pressing function. The tube cap 15 is threadedly connected to the sample storage tube 10. By rotating the tube cap 15, the liquid-pressing plug 14 can be pushed into the sample storage tube 10, allowing the mixture to pass through the injection groove 302 of the injection needle 7.

[0072] The built-in reagent test cartridge structure provided in this embodiment is simpler; in embodiment 1, when the tube cap 15 is flipped to close the second inlet hole 102 of the sample storage tube 10, the user can easily push the push valve 11, thereby discarding the entire built-in reagent test cartridge. In this embodiment, the thread can limit both the initial and final positions of the tube cap 15, effectively preventing accidental touch by the user.

[0073] Example 5

[0074] The main difference between this embodiment and Embodiment 1 is that: Figure 11As shown, six first sample injection holes 101 are formed in the sample storage tube 10. The tube cover 15 is rotatably connected to the end of the sample storage tube 10.

[0075] A partition plate 19 is fixedly connected in the sample storage tube 10. The partition plate 19 is uniformly provided with a plurality of second sample injection holes 102 in communication. The partition plate 19 is used to disperse pressure, separate several liquid pressing plugs 14, and prevent the volume of one liquid pressing plug from being too large to affect the effect of liquid pressing and sealing.

[0076] The liquid pressing member includes a screw rod 20, a liquid injection nut 21, a screw cap 22, a pressing plate 23, and a pressing rod 24. The screw rod 20 is connected to the tube cover 15 along the center line direction of the tube cover 15. The screw rod 20 is threadedly connected with the liquid injection nut 21, and the liquid injection nut 21 is located outside the tube cover 15. The liquid injection nut 21 is fixedly connected with the screw cap 22. In this embodiment, the liquid injection nut 21 and the screw cap 22 are fixed by glue. The tube cover 15 and the screw rod 20 can also be integrally connected or separately processed and then fixedly connected.

[0077] The pressing plate 23 is sleeved on the rod body of the screw rod 20 and is located outside the tube cover 15. One side of the pressing plate 23 is connected with the pressing rod 24 whose free end extends into the sample storage tube 10, and the other side of the pressing plate 23 abuts against the liquid injection nut 21. The free end of the pressing rod 24 is connected with the liquid pressing plug 14. In this embodiment, the liquid pressing plug 14 is sleeved on the free end of the pressing rod 24, and the liquid pressing plug 14 is in interference sealing tight fit connection with the sample storage tube 10 and the partition plate 19 in the second sample injection hole 102.

[0078] During detection, the user first takes out the sample tube assembly 18, tears the outer sealing film 13, adds blood or serum sample into the sample storage tube 10, rotates the tube cover 15, and buckles it on the sample storage tube 10 to mix the sample and the sample storage liquid to obtain a mixed liquid. Then, the tube cover 15 is rotated to cover the sample storage tube 10. The inner sealing film 12 is torn, the reaction chip assembly 17 and the sample tube assembly 18 are connected, the liquid injection nut 21 is rotated, the liquid injection nut 21 drives the pressing plate 23 and the pressing rod 24 to move into the sample storage tube 10, thereby driving the liquid pressing plug 14 to push the mixed liquid into the silicon-based chip 1. The fine adjustment thread structure in this embodiment can better control the liquid injection rhythm and effectively prevent the entire built-in reagent detection card box from being discarded due to accidental user touch.

[0079] The preferred embodiments of the present application are described in detail above. It should be understood that those of ordinary skill in the art can make modifications and changes to the embodiments without creative effort based on the concept of the present application. Therefore, any technical solutions obtained by logical analysis, reasoning or limited experiments based on the prior art according to the concept of the present application shall be within the protection scope defined by the claims.

Claims

1. A silicon-based chip-based built-in reagent detection cartridge, characterized by, The application relates to a sample collection and reaction device, which comprises a reaction chip assembly (17) and a sample tube assembly (18) assembled together; the reaction chip assembly (17) comprises a silicon-based chip (1), a chip seat (3), a sealing block (6) and a sample injection needle (7); the sample tube assembly (18) comprises a sample storage tube (10) and a liquid pressing member; the silicon-based chip (1), the chip seat (3), the sample injection needle (7) and the sample storage tube (10) are sequentially connected. A plurality of holes are formed on the silicon-based chip (1), the number of the holes is even, two adjacent holes form a group, one hole is a sample injection port, and the other hole is an exhaust port; an exhaust hole (701) is formed on the sample injection needle (7); the sealing block (6) is arranged in the exhaust hole (701). A slot (301), a sample injection groove (302) and a sample outlet groove (303) are formed on the chip seat (3); the slot (301) is used for connecting the silicon-based chip (1) and the chip seat (3), a freeze-dried reagent (4) is pre-installed in the sample injection groove (302), the sample injection groove (302) is communicated with the sample injection port of the silicon-based chip (1) and the needle hole of the sample injection needle (7), and the sample outlet groove (303) is communicated with the exhaust port of the silicon-based chip (1) and the exhaust hole (701). The sample storage tube (10) is pre-installed with a sample storage liquid, a first sample injection hole (101) is formed on the sample storage tube (10), the sample injection needle (7) is inserted into the first sample injection hole (101), and the liquid in the sample storage tube (10) is pushed into the silicon-based chip (1) by the liquid pressing member. After a sample is collected in the sample storage tube (10), the sample injection needle (7) is inserted into the sample storage tube (10) to connect the sample injection needle (7) and the sample storage tube (10); a rubber tube (8) is sleeved on the sample injection needle (7), and the sealing block (6) is self-sealing.

2. The silicon-based chip-based built-in reagent detection cartridge according to claim 1, characterized in that, A sealing strip (2) is arranged between the silicon-based chip (1) and the chip seat (3), the sealing strip (2) is provided with connecting holes, and a sealing rib (16) extending outward and matched with the holes of the silicon-based chip (1) is arranged on each connecting hole.

3. The silicon-based chip-based built-in reagent detection card cartridge according to claim 2, characterized in that, The size of the sealing rib (16) is larger than that of the sealing groove by 0.1 mm at most.

4. The silicon-based chip-based built-in reagent detection cartridge according to claim 1 or 2, characterized by, A needle seat sealing gasket (5) is arranged between the chip seat (3) and the sample injection needle (7), and sealing grooves are arranged on the sample injection groove (302) and the sample outlet groove (303); the sealing rib (16) extending outward and matched with the sealing grooves is arranged on the needle seat sealing gasket (5).

5. The silicon-based chip-based built-in reagent detection card kit according to claim 1, characterized in that, A second sample injection hole (102) for sample entering is further formed on the sample storage tube (10), the number of the second sample injection hole (102) is one or more, and the plurality of second sample injection holes (102) are communicated or not communicated.

6. The silicon-based chip-based built-in reagent detection card kit according to claim 5, characterized in that, The application further comprises a tube cover (15) capable of sealing the second sample injection hole (102).

7. The silicon-based chip-based built-in reagent detection card kit according to claim 1 or 6, characterized in that, The liquid pressing member comprises a liquid pressing plug (14), and the liquid pressing plug (14) is connected in tight fit with the inner wall of the sample storage tube (10) through interference sealing.

8. The silicon-based chip-based built-in reagent detection card kit according to claim 7, characterized in that, The liquid pressing member comprises a pushing valve (11), a through hole is formed on the tube cover (15), one end of the pushing valve (11) passes through the through hole and is buckled with the liquid pressing plug (14), a handle and a limiting protrusion (1101) capable of passing through the through hole are arranged on the pushing valve (11), the handle is arranged outside the tube cover (15), and the size of the handle and the limiting protrusion (1101) is larger than that of the through hole.

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