Preparation method of worm manure organic fertilizer

Abstract

The application discloses a preparation method of insect manure organic fertilizer, which comprises the following materials: test wheat bran with a particle size of 150 um, fermentation feed and black soldier fly insect manure. The application takes black soldier fly combined with microorganism processing of pineapple processing waste as the object, studies the influence of feed proportion with pineapple processing waste as the main feeding raw material on the growth performance of black soldier fly, the nutritional quality of black soldier fly dry insects, the conversion effect of pineapple processing waste and the reduction rate, improves the utilization rate of pineapple processing waste, and obtains suitable breeding black soldier fly larvae feed proportion, so that the black soldier fly larvae obtain higher social value and ecological benefits.

Description

Technical Field

[0001] This invention relates to the field of insect excrement organic fertilizer, specifically a method for preparing insect excrement organic fertilizer. Background Technology

[0002] Black soldier flies are holometabolous insects, with a life cycle consisting of four stages: egg, larva, pupa, and adult. They are rich in nutrients; research reports indicate that the dry matter content of black soldier fly larvae can reach approximately 42%. After processing, black soldier flies can be used as feed to produce high-quality insect protein. The crude fat content in black soldier fly larvae is as high as 30% to 40%, and the larvae can accumulate oils that can be used as raw materials for biodiesel production. When stimulated by external factors, black soldier fly larvae can produce small molecule active peptides with antibacterial capabilities, known as antimicrobial peptides or natural antibiotics.

[0003] In recent years, a new method of utilizing kitchen waste has been developed for resource utilization. By using earthworms, flies, and horseflies to absorb kitchen waste, the resulting fly larvae, earthworms, and horseflies can be used as new animal feed, thus generating relatively high economic value. To date, most studies on black soldier fly farming have used kitchen waste and poultry manure as the main feed, while there are relatively few studies on feeding black soldier flies with pineapple processing waste as the main feed. Summary of the Invention

[0004] The purpose of this invention is to provide a method for preparing insect excrement organic fertilizer to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A method for preparing insect excrement organic fertilizer includes the following steps:

[0007] Step S1, Larval Culture: Take fresh, qualified black soldier fly eggs and incubate them in a rearing box (27×20.5×15cm). Add wheat bran (75% moisture content) to the box.

[0008] Step S2, set the breeding parameters: maintain an ambient temperature of 280℃-300℃ and a relative humidity of 70%-80%, and incubate for 5 days;

[0009] Step S3, observation and treatment: During the hatching period, observe the condition of the wheat bran every day, add an appropriate amount of wheat bran according to the consumption, spray water in small amounts several times, and gently stir until it is soft and moist. Use five-day-old larvae as test insects.

[0010] Step S4, Maintenance and Management: Regularly turn over and manage the culture bed or container to promote the decomposition of organic matter and the formation of insect excrement. At the same time, maintain suitable humidity and ventilation conditions to avoid odors and the breeding of pests.

[0011] Step S5, Harvesting and Processing: Harvest the insect excrement as needed, remove the insect bodies and undecomposed organic matter, and carry out subsequent processing, such as drying, screening and crushing, to obtain the finished insect excrement organic fertilizer.

[0012] Step S6, Experiment and Grouping: Set up 8 experimental groups, namely T1, T2, T3, T4, T5, T6, CK1 and CK2. Place the 8 experimental samples into 8 plastic rearing boxes with a size of 27×20.5×15cm. Each group has 3 replicates, and each replicate is 1.5g of 5-day-old larvae. Place 24 samples of 1.5g of 5-day-old black soldier fly larvae into the plastic boxes. The experimental period is 12 days, and the 5-day-old larvae are used as test insects.

[0013] Step S7, Results and Analysis: The effects of different feed ratios on the growth and development of black soldier flies, the nutritional quality of dried black soldier flies, and the conversion efficiency of pineapple processing by-products were analyzed.

[0014] Step S8, Discussion and Research: The effects of feed formulation with pineapple processing waste as the main feed ingredient on the growth performance of black soldier flies, the nutritional quality of dried black soldier fly larvae, the conversion effect of pineapple processing waste, and the reduction rate.

[0015] Furthermore, in step S6, the experimental larvae rearing method is as follows:

[0016] Use a sand sieve to screen out a number of larvae of uniform age and similar size. Then select a number of healthy, active, and uniform 5-day-old larvae, take 1.5g from each of the 24 replicates, and randomly distribute them into 24 breeding boxes of uniform size. Check daily and ensure feed supply.

[0017] On the first day of the experiment, 1 kg of feed was added to each rearing box. The changes in larval feeding were observed in the later stages. During the experiment, the same temperature, humidity and light conditions were maintained in each rearing box. After each larval record was made, an appropriate amount of feed was added.

[0018] Every two days, 20 clean black soldier fly larvae were randomly selected from each treatment. The clean larvae were weighed and recorded using an electronic analytical balance before being returned to their original positions. In each replicate experiment, when half of the larvae showed discoloration, feeding was stopped, and relevant data were recorded.

[0019] Furthermore, in step S6, the growth and development indicators of the black soldier fly include:

[0020] Survival rate = (Number of survivors / Initial stocking quantity) * 100%

[0021] Total output = Final dry weight of black soldier fly larvae - Initial dry weight of black soldier fly larvae;

[0022] Daily weight gain = Total yield / Number of feeding days

[0023] Furthermore, in step S6, the nutritional components of the dried black soldier fly larvae include:

[0024] Nitrogen-free extract of black soldier fly: GB / T 10647-2008;

[0025] Ash content: GB / T 6438-2007;

[0026] Total phosphorus: GB / T 6437-2018;

[0027] Crude protein: GB / T 6432-2018;

[0028] Crude fat: GB / T 6433-2006;

[0029] Crude fiber: GB / T T6434-2006;

[0030] Calcium: GB / T 6436-2018.

[0031] Furthermore, in step S6, the conversion efficiency indicators for pineapple processing waste are as follows:

[0032] Moisture content = [(Wet weight of pineapple processing waste - Dry weight of pineapple processing waste)] / Wet weight of pineapple processing waste × 100%;

[0033] Pineapple processing waste reduction rate = [Pineapple processing waste mass (dry weight) - Residue mass (dry weight)] / Pineapple processing waste mass (dry weight) × 100%;

[0034] Feed conversion ratio = (total dry weight of feed - total dry weight of insects and sand) × 10³ / (fresh weight of pre-pupae × 42%);

[0035] Pineapple processing waste conversion rate = Increased insect body mass (dry weight) / Converted pineapple processing waste mass (dry weight) × 100%.

[0036] Furthermore, in step S8, the use of microbial fermented feed and the addition of corn flour to the feed significantly improved the body weight, total yield, and average daily weight gain of black soldier flies to varying degrees. Among them, group T5, which was fed black soldier flies with a feed ratio of 60% pineapple residue, 30% wheat bran, 10% corn flour, and 0.1% fermentation agent, was better than groups T2 and CK2 in terms of total yield and average daily weight gain than groups that were naturally fermented and whose feed did not contain corn flour.

[0037] Furthermore, in step S8, there was no significant difference between group T1 (80% pineapple processing waste feed ratio), group T3 (70% pineapple processing waste feed ratio), and group T5 (60% pineapple processing waste feed ratio). However, there was a certain difference in the daily yield and average daily weight gain of black soldier flies. This indicates that a feed ratio of 60% to 70% pineapple processing waste is more suitable for the healthy growth and development of black soldier flies than a feed ratio of 80% pineapple processing waste.

[0038] Furthermore, in step S8, the nutritional effects of black soldier flies as feed are mainly as follows: as a protein source, they can replace fishmeal, promote feeding and growth, and enhance the antioxidant capacity and non-specific immunity of fish.

[0039] Furthermore, in step S8, pineapple processing waste can promote the growth and development of black soldier flies. The effectiveness of the conversion of pineapple processing waste is determined by the feeding efficiency of black soldier flies on the pineapple processing waste. The reduction rate of pineapple processing waste is related to the weight of pineapple processing waste, and pineapple processing waste itself contains certain nutrients.

[0040] Compared with the prior art, the beneficial effects of the present invention are:

[0041] This invention focuses on the combined treatment of pineapple processing waste using black soldier fly larvae and microorganisms. It investigates the effects of feed formulations using pineapple processing waste as the main feed ingredient on the growth performance, nutritional quality of dried black soldier fly larvae, conversion efficiency, and waste reduction rate of black soldier fly larvae. The aim is to improve the utilization rate of pineapple processing waste while obtaining a suitable feed formulation for raising black soldier fly larvae, thereby achieving higher social value and ecological benefits. Attached Figure Description

[0042] Figure 1 This is a schematic diagram of the preparation method of an insect excrement organic fertilizer according to the present invention;

[0043] Figure 2 This is a schematic diagram illustrating the effect of different feed ratios on the weight changes of black soldier fly larvae in the preparation method of an insect excrement organic fertilizer according to the present invention.

[0044] Figure 3 This is a schematic diagram illustrating the effect of different feed ratios on the survival rate of black soldier fly larvae in a method for preparing insect excrement organic fertilizer according to the present invention.

[0045] Figure 4 This is a schematic diagram illustrating the effect of different feed ratios on the feed-to-weight ratio during the rearing process of black soldier fly larvae in a method for preparing insect excrement organic fertilizer according to the present invention.

[0046] Figure 5This is a schematic diagram illustrating the effect of different feed ratios on the conversion rate of pineapple processing waste in the preparation method of an insect excrement organic fertilizer according to the present invention.

[0047] Figure 6 This diagram illustrates the effect of different feed ratios on the waste reduction rate in pineapple processing during the preparation of an insect excrement organic fertilizer according to the present invention. Detailed Implementation

[0048] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0049] Please see Figure 1 The present invention provides a technical solution:

[0050] A method for preparing insect excrement organic fertilizer includes the following steps:

[0051] Step S1, Larval Culture: Take fresh, qualified black soldier fly eggs and incubate them in a rearing box (27×20.5×15cm). Add wheat bran (75% moisture content) to the box.

[0052] Step S2, set the breeding parameters: maintain an ambient temperature of 280℃-300℃ and a relative humidity of 70%-80%, and incubate for 5 days;

[0053] Step S3, observation and treatment: During the hatching period, observe the condition of the wheat bran every day, add an appropriate amount of wheat bran according to the consumption, spray water in small amounts several times, and gently stir until it is soft and moist. Use five-day-old larvae as test insects.

[0054] Step S4, Maintenance and Management: Regularly turn over and manage the culture bed or container to promote the decomposition of organic matter and the formation of insect excrement. At the same time, maintain suitable humidity and ventilation conditions to avoid odors and the breeding of pests.

[0055] Step S5, Harvesting and Processing: Harvest the insect excrement as needed, remove the insect bodies and undecomposed organic matter, and carry out subsequent processing, such as drying, screening and crushing, to obtain the finished insect excrement organic fertilizer.

[0056] Step S6, Experiment and Grouping: Set up 8 experimental groups, namely T1, T2, T3, T4, T5, T6, CK1 and CK2. Place the 8 experimental samples into 8 plastic rearing boxes with a size of 27×20.5×15cm. Each group has 3 replicates, and each replicate is 1.5g of 5-day-old larvae. Place 24 samples of 1.5g of 5-day-old black soldier fly larvae into the plastic boxes. The experimental period is 12 days, and the 5-day-old larvae are used as test insects.

[0057] The composition of the fermented feeds in each group is shown in Table 1:

[0058] Table 1. Composition ratio of feeding materials for each group of black soldier fly larvae

[0059] Grouping Pineapple peel residue (80% moisture content) Wheat bran (moisture content approximately 10%) Corn flour (moisture content approximately 10%) Fermentation agent T1 80% 10% 10% 0.10% T2 80% 20% 0 0.10% T3 70% 20% 10% 0.10% T4 70% 30% 0 0.10% T5 60% 30% 10% 0.10% T6 50% 40% 10% 0.10% CK1 60% 30% 10% 0 CK2 70% 30% 0 0

[0060] Step S7, Results and Analysis: The effects of different feed ratios on the growth and development of black soldier flies, the nutritional quality of dried black soldier flies, and the conversion efficiency of pineapple processing by-products were analyzed.

[0061] Step S8, Discussion and Research: The effects of feed formulation with pineapple processing waste as the main feed ingredient on the growth performance of black soldier flies, the nutritional quality of dried black soldier fly larvae, the conversion effect of pineapple processing waste, and the reduction rate.

[0062] During the larval stage of black soldier fly larvae from 5 to 17 days old, the growth differences in fermented feed containing different proportions of pineapple processing waste and bran and corn flour showed certain regularity. When the content of pineapple processing waste was 60%-80%, the weight, total yield, and average daily weight gain of black soldier fly larvae were higher, and the nutritional composition of the black soldier fly larvae also met the standards.

[0063] During the conversion of black soldier flies using a mixed fermented feed of pineapple processing waste and wheat bran and corn flour at different proportions, group T1, fed with a mixed fermented feed of 80% pineapple processing waste, 10% wheat bran, 10% corn flour, and 0.1% fermentation agent, showed the best conversion rate, weight loss rate, and feed conversion ratio. The difference in conversion rate, weight loss rate, and feed conversion ratio between group T1 and group T3, fed with a mixed fermented feed of 70% pineapple processing waste, 20% wheat bran, 10% corn flour, and 0.1% fermentation agent, was not significant. Given that the price of pineapple processing waste is much lower than that of wheat bran, the feed cost of group T1 was lower than that of group T3. Therefore, group T1's feed formulation combined with microbial treatment of pineapple processing waste yielded the best economic and ecological benefits.

[0064] In this invention, the experimental larvae rearing method in step S6 is as follows:

[0065] Use a sand sieve to screen out a number of larvae of uniform age and similar size. Then select a number of healthy, active, and uniform 5-day-old larvae, take 1.5g from each of the 24 replicates, and randomly distribute them into 24 breeding boxes of uniform size. Check daily and ensure feed supply.

[0066] On the first day of the experiment, 1 kg of feed was added to each rearing box. The changes in larval feeding were observed in the later stages. During the experiment, the same temperature, humidity and light conditions were maintained in each rearing box. After each larval record was made, an appropriate amount of feed was added.

[0067] Every two days, 20 clean black soldier fly larvae were randomly selected from each treatment. The clean larvae were weighed and recorded using an electronic analytical balance before being returned to their original positions. In each replicate experiment, when half of the larvae showed discoloration, feeding was stopped, and relevant data were recorded.

[0068] In this invention, the growth and development indicators of black soldier fly larvae in step S6 include:

[0069] Survival rate = (Number of survivors / Initial stocking quantity) * 100%

[0070] Total output = Final dry weight of black soldier fly larvae - Initial dry weight of black soldier fly larvae;

[0071] Daily weight gain = Total yield / Number of feeding days

[0072] In this invention, the nutritional components of dried black soldier fly larvae in step S6 include:

[0073] Nitrogen-free extract of black soldier fly: GB / T 10647-2008;

[0074] Ash content: GB / T 6438-2007;

[0075] Total phosphorus: GB / T 6437-2018;

[0076] Crude protein: GB / T 6432-2018;

[0077] Crude fat: GB / T 6433-2006;

[0078] Crude fiber: GB / T T6434-2006;

[0079] Calcium: GB / T 6436-2018.

[0080] In this invention, the conversion efficiency index of pineapple processing waste in step S6 is as follows:

[0081] Moisture content = [(Wet weight of pineapple processing waste - Dry weight of pineapple processing waste)] / Wet weight of pineapple processing waste × 100%;

[0082] Pineapple processing waste reduction rate = [Pineapple processing waste mass (dry weight) - Residue mass (dry weight)] / Pineapple processing waste mass (dry weight) × 100%;

[0083] Feed conversion ratio = (total dry weight of feed - total dry weight of insects and sand) × 10³ / (fresh weight of pre-pupae × 42%);

[0084] Pineapple processing waste conversion rate = Increased insect body mass (dry weight) / Converted pineapple processing waste mass (dry weight) × 100%.

[0085] In this invention, in step S8, the use of microbial fermented feed and the addition of corn flour to the feed significantly improved the body weight, total yield, and average daily weight gain of black soldier flies to varying degrees. Among them, group T5, which was fed black soldier flies with a feed ratio of 60% pineapple residue, 30% wheat bran, 10% corn flour, and 0.1% fermentation agent, was better than groups T2 and CK2 in terms of total yield and average daily weight gain than groups that were naturally fermented and whose feed did not contain corn flour.

[0086] Table 2 Effects of different feed ratios on the growth and development of black soldier flies

[0087] Grouping body weight / g Total output / g Average daily weight gain (g / d) T1 0.23±0.01a 77.86±9.33ab 6.49±0.78ab T2 0.19±0.01b 59.25±4.49b 4.94±0.37b T3 0.23±0.01a 90.76±10.39a 7.56±0.87a T4 0.26±0.02a 58.95±16.62b 4.91±1.38b T5 0.22±0.02a 92.85±14.84a 7.74±1.24a T6 0.22±0.01a 69.89±3.99ab 5.82±0.33ab CK1 0.29±0.01a 89.62±7.39a 7.47±0.62a CK2 0.30±0.01a 55.77±1.83b 4.65±0.15b

[0088] During the transformation process, the trends in black soldier fly weight change and the survival rate of black soldier fly larvae, such as... Figure 2 , Figure 3 As shown, by Figure 2 , Figure 3It can be seen that the critical growth period for black soldier fly larvae is 4 to 6 days, and the survival rate of black soldier fly larvae is between 85% and 93%. The survival rate is highest in the CK1 group and lowest in the T4 group, with a difference of 7.33%. However, there is no significant difference in survival rate among the groups. The growth and development of black soldier flies after conversion is shown in Table 1. Table 2 shows that after conversion, the weight of black soldier flies in each group ranged from 0.19 g to 0.31 g. The T2 group had the lowest weight, decreasing by 0.1 g compared to the CK1 group and by 0.11 g compared to the CK2 group. The weight of black soldier flies in the T2 group was significantly different from that in the T1, T3, T4, T5, T6, CK1, and CK2 groups. There were no significant differences among the other groups. Regarding the total yield of black soldier flies, the total yield of each group... The yield ranged from 55 grams to 93 grams, with group T5 yielding the most and group CK2 yielding the least, a difference of 37.08 grams. Regarding the total yield of black soldier flies, the total yield of each group ranged from 55.77 grams to 92.85 grams. There were significant differences between groups CK1, T3, and T5 and groups CK2, T2, and T4, while the former and the latter did not differ significantly from groups T1 and T6. As for the average daily weight gain of black soldier flies, the average daily weight gain of each group ranged from 4.65 grams to 7.74 grams, with group T5 yielding the most and group CK2 yielding the least, a difference of 3.09 g / day. Similarly, there were no significant differences between groups CK1, T3, and T5 and groups T1 and T6 in terms of average daily weight gain, and similarly, there were no significant differences between groups CK2, T2, and T4 and groups T1 and T6. However, there were significant differences between groups CK1, T3, and T5 and groups CK2, T2, and T4.

[0089] In this invention, in step S8, there was no significant difference between group T1 (80% pineapple processing waste feed ratio), group T3 (70% pineapple processing waste feed ratio), and group T5 (60% pineapple processing waste feed ratio). However, there was a certain difference in the daily yield and average daily weight gain of black soldier flies. This indicates that a feed ratio of 60% to 70% pineapple processing waste is more suitable for the healthy growth and development of black soldier flies than a feed ratio of 80% pineapple processing waste.

[0090] Table 3. Effects of different feed ratios on the nutritional quality of dried black soldier fly larvae.

[0091] Testing items Nitrogen-free extract Ash Total phosphorus crude protein Crude fat crude fiber calcium Grouping % % % % g / kg g / kg % T1 3.9 4.9 0.66 45.18 348 74 0.62 T2 3.7 5.8 0.7 48.58 299 88 0.71 T3 3.7 4.5 0.61 46.56 304 113 0.41 T4 0 5 0.65 48.65 328 108 0.6 T5 0 4.5 0.66 47.26 351 101 0.44 T6 2.8 4.5 0.65 45.55 361 67 0.43 CK1 4.4 4.6 0.66 44.86 350 61 0.45 CK2 0 5.6 0.76 49.59 332 107 0.69

[0092] After the rearing period, the composition of dried black soldier fly larvae was shown in Table 3. Regarding nitrogen-free extract content, the content of nitrogen-free extract in each group ranged from 0% to 4.4%, with groups T4, T5, and CK2 all having a content of 0%, while group CK2 had the highest content at 4.4%. Regarding crude protein content, the content of crude protein in each group ranged from 44.86% to 49.59%, with group CK2 having the highest content and group CK1 having the lowest, a difference of 4.73 percentage points. Regarding crude fat content, the content of crude fat in each group ranged from 299 g / kg to 361 g / kg, with group T6 having the highest content and group T2 having the lowest, a difference of 62 percentage points. Regarding crude fiber content, the content of crude fiber in each group ranged from 61 g / kg to 113 g / kg, with group T3 having the highest content and group CK1 having the lowest, a difference of 5 percentage points. At 2g / kg, the ash content of black soldier fly larvae ranged from 4.5% to 5.8% across all groups. Group T2 had the highest ash content, with no difference from groups T3, T5, and T6. Groups T3, T5, and T6 had the lowest ash content. Groups CK2 and T2 had higher ash content compared to the other groups. The main component of ash is the oxide of mineral elements, indicating that the black soldier fly larvae in these two groups had a higher content of nutrients. Regarding the calcium content of black soldier fly larvae, the calcium content ranged from 0.41% to 0.71% across all groups. Group T2 had the highest calcium content, followed by group CK2, and group T3 had the lowest calcium content.

[0093] In this invention, the nutritional effects of black soldier fly as feed in step S8 are mainly as follows: as a protein source, it can replace fishmeal, promote feeding and growth, and enhance the antioxidant capacity and non-specific immunity of fish.

[0094] Depend on Figure 4 It can be seen that, regarding the effect of different feed ratios on the feed conversion ratio (FCR) during the rearing process of black soldier fly larvae, the FCR ranged from 9.26 to 24.09, with group T1 having the lowest FCR and group T6 having the highest, a difference of 14.82. Significant differences were found between groups T1, T2, T3, and CK1 and groups T4, T5, T6, and CK2; significant differences were also found between group T5 and groups T6 and CK2; no significant differences were found among groups T1, T2, T3, and CK1; and no significant differences were found among groups T4, T5, T6, and CK2. Figure 5 It can be seen that, regarding the effect of different feed ratios on the conversion rate of pineapple processing waste, the conversion rates of each group ranged from 15.57% to 47.55%, with group T1 having the highest conversion rate and group CK2 having the lowest, a difference of 31.98%. Significant differences were found between group T1 and groups T2, T4, T5, T6, CK1, and CK2, but no significant difference was found between group T1 and group T3. Significant differences were found between groups T2, T5, and CK1 and groups T6 and CK2. No significant differences were found among the three groups T2, T5, and CK1. No significant differences were found between groups T6 and CK2. Figure 6It can be seen that, regarding the effect of different feed ratios on the reduction rate of pineapple processing waste, the reduction rates of each group ranged from 55.67% to 68.00%, with the highest reduction rate in group T1 and the lowest in group CK2, with a difference of 12.33%. There were significant differences between groups T1, T3, T5, CK1, and CK2 and groups T2, T4, and T6, and significant differences between group CK2 and groups T1, T3, T5, CK1, T2, T4, and T6. There were no significant differences among groups T1, T3, T5, CK1, and CK2, and no significant differences among groups T2, T4, and T6.

[0095] In this invention, in step S8, pineapple processing waste can promote the growth and development of black soldier flies. The effectiveness of the conversion of pineapple processing waste is determined by the feeding efficiency of black soldier flies on the pineapple processing waste. The reduction rate of pineapple processing waste is related to the weight of pineapple processing waste, and pineapple processing waste itself contains certain nutrients.

[0096] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0097] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for preparing worm cast organic fertilizer, characterized by: The method comprises the following steps: Step S1, take fresh qualified black soldier fly eggs, place them in a feeding box with dimensions of 27x20.5x15cm, and add wheat bran with a moisture content of 75% and a particle size of 150um to the box; Step S2, maintain an environmental temperature of 28-30℃ and a relative humidity of 70-80%, and incubate for 5 days; Step S3, observe the condition of the wheat bran every day during incubation, and add an appropriate amount of feed according to the consumption, and use five-day-old larvae as test insects; Step S4, regularly turn and manage the culture bed to promote the decomposition of organic matter and the formation of worm manure, while maintaining appropriate humidity and ventilation conditions to avoid the breeding of odors and pests; Step S5, harvest worm manure as needed, remove worm bodies and undecomposed organic matter, and perform subsequent processing, drying, screening, and crushing to obtain finished worm manure organic fertilizer; Step S6, set up 8 experimental groups, and place 8 experimental samples in 8 plastic feeding boxes, respectively, with the plastic feeding boxes having dimensions of 27x20.5x15cm, 3 repeats in each group, and 1.5g of 5-day-old larvae in each repeat; In the step S6, the test insect feeding method comprises: Step S6-1, screen out several larvae of similar specifications using a sand screen, then select several 5-day-old larvae that are healthy, active, and uniform in size, take 1.5g of larvae for each of the 24 repeats, and randomly distribute them into 24 feeding boxes of uniform size, check and ensure the supply of feed every day; Step S6-2, on the first day of the formal test, add 1kg of feed to each feeding box, observe the changes in larval feeding in the later stage, maintain the same temperature, humidity, and light conditions in each feeding box during the test, and add an appropriate amount of feed after recording the larvae each time; Step S6-3, randomly select 20 clean black soldier fly larvae from each group every two days, weigh and record the clean larvae using an electronic analytical balance, and then put them back, stop adding feed when half of the test insects in each repeat turn color, and record the relevant data; Step S7, analyze the effects of different feed ratios on the growth and development of black soldier flies, the nutritional composition of black soldier fly dry insects, and the conversion effect of pineapple processing waste; Step S8, study the effects of feed ratios with pineapple processing waste as the main feeding raw material on the growth and development of black soldier flies, the nutritional composition of black soldier fly dry insects, and the conversion effect of pineapple processing waste.

2. The preparation method of the worm manure organic fertilizer according to claim 1, characterized in that: In the step S6-3, the relevant data includes: black soldier fly growth and development indicators, black soldier fly dry insect nutrient composition indicators, and pineapple processing waste conversion effect indicators, wherein the black soldier fly growth and development indicators include: Survival rate = survival amount / initial amount * 100%; Total yield = final dry weight of black soldier flies - initial dry weight of black soldier flies; Daily average weight gain = total yield / feeding days; The pineapple processing waste conversion effect indicators include: Moisture content = [(wet weight of pineapple processing waste - dry weight of pineapple processing waste)] / wet weight of pineapple processing waste * 100%; Pineapple processing waste reduction rate = (dry weight of pineapple processing waste - dry weight of residue) / dry weight of pineapple processing waste * 100%; The feed weight ratio = (total dry weight of feed - total dry weight of sand) × 103 / (fresh weight of pre-pupa fly × 42%); The conversion rate of pineapple processing waste = increased dry weight of insect body / dry weight of converted pineapple processing waste × 100%.

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