Anti-aging and whitening compositions, anti-aging and whitening liposomes, face creams and their preparation methods

Abstract

This invention relates to the field of cosmetic technology, and particularly to an anti-aging and whitening composition, an anti-aging and whitening liposome, a face cream, and a method for preparing the same. The anti-aging and whitening composition comprises the following components in parts by weight: 0.1 to 10 parts yeast / rice fermentation product filtrate, 0.1 to 5 parts golden microalgae, and 0.1 to 3 parts β-nicotinamide mononucleotide. The anti-aging and whitening composition of this invention has the advantages of addressing anti-aging issues from multiple dimensions, effectively solving penetration problems, and achieving brightening in a gentle and effective manner from multiple dimensions.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to an anti-aging and whitening composition, an anti-aging and whitening liposome, a face cream, and a method for preparing the same. Background Technology

[0002] Aging is influenced by numerous exogenous stimuli and endogenous cellular factors, resulting in a complex mechanism. Current anti-aging products are concentrated in the food, health supplements, and cosmetic medicine sectors, offering a relatively singular approach to anti-aging. For example, lifting the face and supplementing collagen only address the issue externally, leading to slow effects or difficulty in the active ingredients reaching the deep layers of the skin and cells, resulting in unsustainable results. Furthermore, the use of retinol and its derivatives to promote collagen synthesis is highly irritating and unstable. Summary of the Invention

[0003] Based on this, the present invention provides an anti-aging and whitening composition, an anti-aging and whitening liposome, a face cream, and a preparation method thereof.

[0004] The first aspect of this invention provides an anti-aging and whitening composition, the technical solution of which is as follows:

[0005] An anti-aging and whitening composition comprising the following components in parts by weight:

[0006] 0.1 to 10 parts yeast / rice fermentation product filtrate, 0.1 to 5 parts golden microalgae, and 0.1 to 3 parts β-nicotinamide mononucleotide.

[0007] In some embodiments, the anti-aging and whitening composition comprises the following components in parts by weight:

[0008] 0.1 to 5 parts yeast / rice fermentation product filtrate, 0.1 to 3 parts golden microalgae, and 0.1 to 3 parts β-nicotinamide mononucleotide.

[0009] In some embodiments, the method for preparing the yeast / rice fermentation product filtrate includes the following steps:

[0010] Cooked rice is inoculated with yeast solution and fermented to prepare the yeast / rice fermentation product filtrate.

[0011] The second aspect of this invention provides an anti-aging and skin-whitening liposome, the technical solution of which is as follows:

[0012] An anti-aging and whitening liposome includes a lipid encapsulation layer and an active ingredient located within the lipid encapsulation layer, the active ingredient including the anti-aging and whitening composition as described above.

[0013] In some embodiments, the anti-aging and whitening composition accounts for 60 wt% to 80% of the total mass of the anti-aging and whitening liposomes.

[0014] In some embodiments, the raw material for the lipid coating layer includes phospholipids.

[0015] A third aspect of this invention provides a face cream, the technical solution of which is as follows:

[0016] A face cream comprising the following components by weight percentage:

[0017] 0.3wt% to 18wt% of the anti-aging and whitening composition as described above, or 0.375wt% to 30wt% of the anti-aging and whitening liposomes as described above, 4.5wt% to 16wt% of skin care agent, 6wt% to 20wt% of thickener, 0.01wt% to 0.2wt% of chelating agent, 0.1wt% to 1wt% of skin feel modifier, 0.5wt% to 3wt% of solubilizer and 42wt% to 85wt% of solvent.

[0018] In some embodiments, the face cream comprises the following components by weight percentage: 0.3wt% to 18wt% of the anti-aging and whitening composition, 1wt% to 4wt% of shea butter, 0.5wt% to 3wt% of squalane, 1wt% to 4wt% of tocopheryl acetate, 2wt% to 5wt% of behenol, 1wt% to 4wt% of sodium acrylate / sodium acryloyldimethyl taurate copolymer, 1.5wt% to 5wt% of carbomer, 2wt% to 6wt% of carboxymethyl cellulose, 1wt% to 3wt% of kaolin, 0.5wt% to 2wt% of titanium dioxide, 0.01wt% to 0.2wt% of disodium EDTA, 0.1wt% to 1wt% of isononyl isononanoate, 0.5wt% to 3wt% of polyethylene glycol stearate, and 42wt% to 85wt% of solvent.

[0019] In some embodiments, the face cream comprises the following components by weight percentage:

[0020] 0.375wt%–30wt% of the anti-aging and whitening liposomes, 1wt%–4wt% of shea butter, 0.5wt%–3wt% of squalane, 1wt%–4wt% of tocopheryl acetate, 2wt%–5wt% of behenol, 1wt%–4wt% of sodium acrylate / sodium acryloyldimethyl taurate copolymer, 1.5wt%–5wt% of carbomer, 2wt%–6wt% of carboxymethyl cellulose, 1wt%–3wt% of kaolin, 0.5wt%–2wt% of titanium dioxide, 0.01wt%–0.2wt% of disodium ethylenediaminetetraacetate, 0.1wt%–1wt% of isononyl isononanoate, 0.5wt%–3wt% of polyethylene glycol stearate, and 42wt%–85wt% of solvent.

[0021] The fourth aspect of this invention provides a method for preparing the face cream as described above, the technical solution of which is as follows:

[0022] A method for preparing a face cream as described above includes the following steps:

[0023] The solvent, a portion of the thickener, and the chelating agent are mixed to prepare a first mixture;

[0024] A second mixture is prepared by mixing the first mixture, the remaining thickener, skin care agent, skin feel modifier, and anti-aging and whitening composition or anti-aging and whitening liposomes.

[0025] Mix the second mixture with the solubilizer and emulsify.

[0026] Compared with traditional solutions, the present invention has the following advantages:

[0027] 1. Solving anti-aging problems from multiple dimensions

[0028] This invention addresses the endogenous and exogenous mechanisms of aging by working on multiple dimensions, including hydration, moisture retention, enhancing cell vitality, and improving skin matrix elasticity, to achieve anti-aging effects.

[0029] One of the hallmarks of skin aging is moisture loss. Therefore, adequate hydration, while simultaneously enhancing the skin's water-retention capacity, and filling the spaces between epidermal collagen and within the epidermal and dermal structures, thereby strengthening the elasticity of the skin's matrix network, is a crucial direction for anti-aging. Firstly, yeast / rice ferment filtrate possesses excellent permeability and skin affinity. The penetration-enhancing effects of golden microalgae and yeast / rice ferment filtrate can assist other skincare ingredients in subsequent face creams to more effectively achieve their hydrating effects. More importantly, α-KG in yeast / rice ferment filtrate can increase the gene expression of markers such as fibronectin and sphingolipids (important components of cell membranes) in keratinocytes, thereby improving the skin's water-retention capacity and strengthening the skin barrier.

[0030] Simultaneously, at the cellular level, aging is accompanied by a decrease in overall cellular energy metabolism, a decline in epidermal and dermal cell vitality and matrix protein content, an increase in the activity of enzymes that decompose the matrix (represented by MMP-1, an important skin aging-related protease; increased content / activity accelerates collagen breakdown, leading to skin laxity and wrinkles), and an accumulation of cellular inflammation, DNA damage, and accelerated cell death. The synergistic effect of yeast / rice fermentation product filtrate and NMN can effectively enhance cellular energy and regulate the activity of the longevity factor sirtuin, achieving anti-aging. Golden microalgae and yeast / rice fermentation product filtrate can synergistically enhance the tissue and structure of the dermal extracellular matrix, promote keratinocyte regeneration, increase the content of fibrin and other components in the dermal extracellular matrix, and reduce related fibrin-degrading enzymes. Both golden microalgae and NMN can alleviate cellular inflammation, and NMN can repair DNA damage, thereby prolonging cell vitality. At the same time, all three key components can inhibit the production of oxygen free radicals, and golden microalgae can also increase the expression of antioxidant enzymes such as SOD and their genes, synergistically reducing aging caused by oxidative damage at its source.

[0031] 2. Effectively solves the problem of penetration.

[0032] The bio-gold in the golden microalgae helps the active ingredients penetrate and absorb; the yeast / rice ferment filtrate is also a good penetration aid, which can assist other soluble active ingredients in synergistic penetration. The combined effect of the two can enable NMN to penetrate into the skin cells efficiently to participate in activating cell energy and synergistically solve the transdermal problem.

[0033] 3. Multi-dimensional, gentle, and effective brightening

[0034] The three key ingredients used in this invention all possess excellent bioactivity, intervening at the source—melanocytes activated by reactive oxygen species, leading to melanin production. Simultaneously, they protect the skin barrier, promote epidermal cell regeneration, and provide ample hydration, achieving a brightening effect while ensuring gentleness and non-irritation. Detailed Implementation

[0035] The present invention will be further described in detail below with reference to specific embodiments. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the present invention.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

[0037] the term

[0038] Unless otherwise stated or in case of contradiction, the terms or phrases used herein shall have the following meanings:

[0039] In this invention, the selection range of "and / or", "or / and", and "and / or" includes any one of two or more related listed items, as well as any and all combinations of the related listed items. These arbitrary and all combinations include any two related listed items, any more related listed items, or a combination of all related listed items. It should be noted that when at least three items are connected using at least two conjunctions selected from "and / or", "or / and", and "and / or", it should be understood that the technical solution undoubtedly includes technical solutions connected by "logical AND", and also undoubtedly includes technical solutions connected by "logical OR". For example, "A and / or B" includes three parallel solutions: A, B, and A+B. For example, the technical solution of "A, and / or, B, and / or, C, and / or, D" includes any one of A, B, C, and D (that is, a technical solution that is connected by "logical OR"), as well as any and all combinations of A, B, C, and D, that is, combinations of any two or three of A, B, C, and D, and also combinations of all four of A, B, C, and D (that is, a technical solution that is connected by "logical AND").

[0040] In this invention, terms such as "multiple", "various", "multiple times", and "multi-dimensional" are used, unless otherwise specified, to refer to a quantity greater than or equal to 2. For example, "one or more" means one or more types.

[0041] In this invention, the terms "optionally," "optionally," and "optional" refer to options that are optional, meaning they are selected from either "with" or "without." If multiple "optional" options appear in a technical solution, unless otherwise specified and there are no contradictions or mutual constraints, each "optional" option is independent.

[0042] In this invention, the terms "first aspect," "second aspect," "third aspect," and "fourth aspect," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or quantity, nor should they be construed as implicitly indicating the importance or quantity of the indicated technical features. Moreover, "first," "second," "third," and "fourth," etc., serve only as a non-exhaustive enumeration and should be understood not to constitute a closed limitation on quantity.

[0043] In this invention, numerical intervals (i.e., numerical ranges) are involved. Unless otherwise specified, the selected numerical distributions within the aforementioned numerical intervals are considered continuous and include the two endpoints (i.e., the minimum and maximum values) of the numerical range, as well as every value between these two endpoints. Unless otherwise specified, when a numerical interval refers only to integers within that interval, it includes the two endpoint integers of the numerical range, as well as every integer between the two endpoints. In this document, this is equivalent to directly listing every integer. For example, if t is an integer selected from 1 to 10, it means that t is any integer selected from the group of integers consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10. Furthermore, when multiple ranges are provided to describe features or characteristics, these ranges can be merged. In other words, unless otherwise specified, the ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0044] Unless otherwise specified, the temperature parameters in this invention can be either constant temperature treatment or variations within a certain temperature range. It should be understood that the constant temperature treatment allows temperature fluctuations within the precision range controlled by the instrument. Fluctuations are permitted within ranges such as ±5℃, ±4℃, ±3℃, ±2℃, and ±1℃.

[0045] In this invention, % (w / w) and wt% both represent weight percentage, % (v / v) refers to volume percentage, and % (w / v) refers to mass-volume percentage.

[0046] The first aspect of the present invention provides an anti-aging and whitening composition, wherein in one embodiment, the anti-aging and whitening composition comprises the following components in parts by weight:

[0047] 0.1 to 10 parts yeast / rice fermentation product filtrate, 0.1 to 5 parts golden microalgae, and 0.1 to 3 parts β-nicotinamide mononucleotide.

[0048] In this embodiment, the yeast / rice fermentation product filtrate is obtained by fermenting rice as a fermentation substrate with yeast culture in a bioreactor. It contains abundant nutrients such as α-KG, adenosine, amino acids, polysaccharides, and minerals. Studies have shown that α-KG can promote the proliferation of epidermal keratinocytes and increase the expression of mRNA (FLG, SPT, and IVL) of skin water retention and barrier-related genes in keratinocytes. Adenosine provides cellular energy, inhibits the formation of oxygen free radicals, thereby promoting skin cell metabolism and the synthesis of collagen and elastin fibers, thus enhancing skin firmness and elasticity and reducing the appearance of fine lines and wrinkles. The addition of the yeast / rice fermentation product filtrate facilitates efficient and rapid hydration and moisturizing, allowing the active ingredients to penetrate deep into the skin for a long-lasting moisturizing effect. At the same time, it also has excellent skin affinity (the contact angle of yeast / rice fermentation product filtrate is 82°, while that of purified water is 102°; the smaller the contact angle, the better the skin affinity) and permeability (permeability experiments have shown that yeast / rice fermentation product filtrate increases the permeability of active ingredients by 26% compared to purified water), which is beneficial for improving the hydration of the epidermis and achieving a brightening effect.

[0049] Optionally, the method for preparing the yeast / rice fermentation product filtrate includes the following steps:

[0050] Cooked rice is inoculated with yeast solution and fermented to prepare the yeast / rice fermentation product filtrate.

[0051] In this embodiment, the yeast strain in the yeast solution is Saccharomyces veronae.

[0052] Optionally, the fermentation culture temperature is 35°C to 40°C. For example, the fermentation culture temperature is 37°C.

[0053] Optionally, the fermentation culture time is 24h to 72h. For example, the fermentation culture time is 48h.

[0054] Microalgae are algal organisms that can adapt to a variety of extreme environments. The gold microalgae production method employed in this invention utilizes specific cultivation conditions, including light and pH, in a closed bioreactor. Algal extracts are co-cultured with gold, transforming "mineral gold" into low-irritant, high-nutrient, and highly active bio-gold. The gold content in the gold microalgae is approximately 2 mg / L. -1 ~10mg·L -1 Within a certain range. Furthermore, the golden microalgae also contain various polysaccharides, oligosaccharides, chlorophyll, amino acids, proteins, minerals, vitamins, and other active ingredients. In particular, the bio-gold under the influence of a micro-electric field helps the absorption of effective ingredients in skincare products, further enhancing its brightening and anti-aging effects.

[0055] Golden microalgae participate in regulating gene expression in metabolic pathways related to skin pigmentation, thereby regulating enzyme activity and melanin formation, ultimately reducing melanin production and accumulation. Furthermore, golden microalgae possess strong antioxidant properties, neutralizing exogenous free radicals for exogenous antioxidant effects and promoting the expression of antioxidant enzyme genes such as SOD for endogenous antioxidant effects, resulting in anti-aging and melanin reduction. In addition, golden microalgae exhibit highly efficient regenerative capabilities. Studies have shown that it can promote the synthesis of dermal fibers such as collagen, inhibit the activity of the enzyme MMP-1 which degrades dermal fibers, and stimulate the expression of regeneration-related genes, thereby accelerating cell repair and regeneration, achieving skin structure regeneration and reconstruction, and realizing a firming and anti-aging effect.

[0056] β-Nicotinamide mononucleotide (NMN) is a bioactive nucleotide primarily found in the cell nucleus and mitochondria. As the body and cells age, the total amount of NAD+ in the body gradually decreases, leading to the accumulation of chronic inflammation and subsequently causing a series of problems such as reduced mitochondrial activity, telomere shortening, and other signs of aging. NMN can be transported into cells to generate NAD+, and NMN primarily exerts its effects through the NAD+ form. However, NAD+ has a large molecular weight and is unstable, making it difficult for the body to directly absorb and utilize. Therefore, direct NAD+ supplementation is not recommended, making NMN a better choice. NMN is easily absorbed and has excellent solid-state stability, which can increase the body's NAD+ levels, thereby providing cellular energy. Related research on anti-aging and cell-energizing effects has been published in top journals such as Nature and is currently widely used in the international health supplement industry.

[0057] Research results indicate that NMN and its derivative NAD+ can participate in various physiological processes, achieving anti-aging effects from multiple perspectives. It can participate in ATP energy supply and cellular electron transport, maintaining cellular energy. Increased ATP can promote talin1 gene expression, increase cell junction proteins, and strengthen cell connections, thus achieving a lifting and firming effect. It can participate in DNA repair, protecting keratinocytes and enhancing their antioxidant and anti-glycation capabilities. It can activate the longevity factor sirtuin (deacetyltransferase), which in turn activates glutathione through a cascade reaction to reduce reactive oxygen species and maintain telomere activity, thereby exerting anti-aging effects. Furthermore, it can downregulate the expression of pro-inflammatory factors, reducing inflammation and thus resisting skin aging damage caused by inflammation. It also regulates the biological clock to combat circadian rhythm disorders caused by aging (such as hormone secretion cycles and sleep rhythms).

[0058] The three main ingredients mentioned above can each have anti-aging effects as individual components. Golden microalgae and yeast / rice ferment filtrate also have whitening and brightening effects. In this embodiment, the three are combined, resulting in a good synergistic effect for whitening and anti-aging. First, the bio-gold in the golden microalgae helps the active ingredients penetrate and absorb. The yeast / rice ferment filtrate itself has good permeability and can assist other soluble active ingredients in synergistic penetration. The combined effect of both allows NMN to efficiently penetrate into skin cells to participate in processes such as activating cellular energy and regulating the activity of the longevity factor sirtuin. In particular, the α-KG and adenosine components in the yeast / rice ferment filtrate are also part of the tricarboxylic acid cycle, participating in cellular energy metabolism along with NAD+. The synergistic effect of both with NMN can more effectively activate cellular energy metabolism and regulate the activity of the longevity factor sirtuin, achieving anti-aging. In addition, the active ingredients in golden microalgae and α-KG in yeast / rice fermentation product filtrate have both been shown to reduce the expression of matrix metalloproteinase MMP-1, which can synergistically enhance the tissue and structure of the extracellular matrix of dermal cells and promote the regeneration of keratinocytes.

[0059] The anti-aging and whitening composition of this embodiment has the advantages of solving anti-aging problems from multiple dimensions, effectively solving penetration problems, and achieving brightening in a gentle and effective manner from multiple dimensions. Specifically:

[0060] 1. Solving anti-aging problems from multiple dimensions

[0061] This invention addresses the endogenous and exogenous mechanisms of aging by working on multiple dimensions, including hydration, moisture retention, enhancing cell vitality, and improving skin matrix elasticity, to achieve anti-aging effects.

[0062] One of the hallmarks of skin aging is moisture loss. Therefore, adequate hydration, while simultaneously enhancing the skin's water-retention capacity, and filling the spaces between epidermal collagen and within the epidermal and dermal structures, thereby strengthening the elasticity of the skin's matrix network, is a crucial direction for anti-aging. Firstly, yeast / rice ferment filtrate possesses excellent permeability and skin affinity. The penetration-enhancing effects of golden microalgae and yeast / rice ferment filtrate can assist other skincare ingredients in subsequent face creams to more effectively achieve their hydrating effects. More importantly, α-KG in yeast / rice ferment filtrate can increase the gene expression of markers such as fibronectin and sphingolipids (important components of cell membranes) in keratinocytes, thereby improving the skin's water-retention capacity and strengthening the skin barrier.

[0063] Simultaneously, at the cellular level, aging is accompanied by a decrease in overall cellular energy metabolism, a decline in epidermal and dermal cell vitality and matrix protein content, an increase in the activity of enzymes that decompose the matrix (represented by MMP-1, an important skin aging-related protease; increased content / activity accelerates collagen breakdown, leading to skin laxity and wrinkles), and an accumulation of cellular inflammation, DNA damage, and accelerated cell death. The synergistic effect of yeast / rice fermentation product filtrate and NMN can effectively enhance cellular energy and regulate the activity of the longevity factor sirtuin, achieving anti-aging. Golden microalgae and yeast / rice fermentation product filtrate can synergistically enhance the tissue and structure of the dermal extracellular matrix, promote keratinocyte regeneration, increase the content of fibrin and other components in the dermal extracellular matrix, and reduce related fibrin-degrading enzymes. Both golden microalgae and NMN can alleviate cellular inflammation, and NMN can repair DNA damage, thereby prolonging cell vitality. At the same time, all three key components can inhibit the production of oxygen free radicals, and golden microalgae can also increase the expression of antioxidant enzymes such as SOD and their genes, synergistically reducing aging caused by oxidative damage at its source.

[0064] 2. Effectively solves the problem of penetration.

[0065] The bio-gold in the golden microalgae helps the active ingredients penetrate and absorb, while the yeast / rice fermentation product filtrate is also a good penetration aid, which can assist other soluble active ingredients in synergistic penetration. The combined effect of the two can enable NMN to penetrate into the skin cells efficiently to participate in activating cell energy and synergistically solve the transdermal problem.

[0066] 3. Multi-dimensional, gentle, and effective brightening

[0067] Many skin-whitening products use unstable and easily decomposed ingredients, such as Vitamin C, or 377, which are irritating or even toxic and can easily cause adverse skin reactions. Furthermore, many products only target a specific stage of melanin development, failing to fundamentally block melanin production and development. For example, whitening methods like acid peels and exfoliation only work on the stratum corneum, with short-lasting effects and strong irritation. Effective whitening ingredients often have some degree of irritation; for instance, niacinamide requires tolerance building and is not suitable for a wide range of people. This invention utilizes three key ingredients, all with good bioactivity, intervening at the source—the activation of melanocytes by reactive oxygen species and the production of melanin. Simultaneously, it protects the skin barrier, promotes epidermal cell regeneration, and provides ample hydration, achieving a brightening effect while ensuring gentleness and non-irritation.

[0068] The weight parts of the yeast / rice fermentation product filtrate include, but are not limited to, 0.1 parts, 3 parts, 5 parts, and 10 parts.

[0069] The weight portions of golden microalgae include, but are not limited to, 0.1 parts, 1 part, 3 parts, and 5 parts.

[0070] The weight parts of β-nicotinamide mononucleotide include, but are not limited to, 0.1 parts, 1 part, 2 parts, and 3 parts.

[0071] Preferably, the anti-aging and whitening composition comprises the following components in parts by weight:

[0072] 0.1 to 5 parts yeast / rice fermentation product filtrate, 0.1 to 3 parts golden microalgae, and 0.1 to 3 parts β-nicotinamide mononucleotide.

[0073] More preferably, the anti-aging and whitening composition comprises the following components in parts by weight:

[0074] 0.1 to 5 parts yeast / rice fermentation product filtrate, 0.1 to 3 parts golden microalgae, and 0.1 to 3 parts β-nicotinamide mononucleotide.

[0075] The anti-aging and whitening composition of the present invention not only has anti-aging effects, but also achieves whitening effects at the same time. The active ingredients can reach deep into the skin and into the cells, have a long-lasting effect, and are non-irritating and stable.

[0076] A second aspect of the present invention provides an anti-aging and whitening liposome. In one embodiment, the anti-aging and whitening liposome includes a lipid encapsulation layer and an active ingredient located within the lipid encapsulation layer, the active ingredient including the aforementioned anti-aging and whitening composition.

[0077] This embodiment employs liposome encapsulation technology to encapsulate the aforementioned anti-aging and whitening composition within a lipid layer, exhibiting excellent biocompatibility. The fusion of the liposome membrane with the biological membrane allows the contained active ingredients to be released intracellularly to exert their effects. This further addresses the transdermal absorption issue, enabling whitening, brightening, and NMN to penetrate deep into the basal cells, achieving a fundamental anti-aging effect. Simultaneously, liposome encapsulation protects the unstable NMN component in the mixed solution, and also allows for sustained release, extending its effective duration, ensuring safety, and achieving long-lasting anti-aging effects.

[0078] Optionally, the anti-aging and whitening composition accounts for 60 wt% to 80% of the total mass of the anti-aging and whitening liposomes. For example, the anti-aging and whitening composition accounts for 70% of the total mass of the anti-aging and whitening liposomes.

[0079] Optionally, the particle size of the anti-aging and whitening liposomes is 0.1 μm to 1 μm.

[0080] Optionally, the raw material for the lipid coating layer includes phospholipids.

[0081] Alternatively, the phospholipid may be lecithin.

[0082] The application of NMN in anti-aging health products is gaining popularity, but its application in anti-aging cosmetics is relatively rare. Furthermore, NMN is unstable in mixed solutions, and commercially available products often use methods such as freeze-drying or encapsulation to preserve its stability in a solid state, which limits its application in emulsion products. This embodiment utilizes liposome encapsulation technology to prepare anti-aging and whitening liposomes, leveraging their affinity for cell membranes to improve permeability, while simultaneously solving the problem of instability when NMN is directly added to aqueous solutions or emulsions.

[0083] A third aspect of the present invention provides a face cream, in one embodiment of which the face cream comprises the following components by weight percentage:

[0084] 0.3wt% to 18wt% of the anti-aging and whitening composition as described above, or 0.375wt% to 30wt% of the anti-aging and whitening liposomes as described above, 4.5wt% to 16wt% of skin care agent, 6wt% to 20wt% of thickener, 0.01wt% to 0.2wt% of chelating agent, 0.1wt% to 1wt% of skin feel modifier, 0.5wt% to 3wt% of solubilizer and 42wt% to 85wt% of solvent.

[0085] The weight percentages of the anti-aging and whitening compositions include, but are not limited to, 0.3 wt%, 1 wt%, 5 wt%, 10 wt%, and 18 wt%.

[0086] The weight percentages of anti-aging and whitening liposomes include, but are not limited to, 0.375 wt%, 1 wt%, 5 wt%, 10 wt%, and 30 wt%.

[0087] The weight percentage of the skin care agent includes, but is not limited to, 4.5 wt%, 10 wt%, and 16 wt%.

[0088] The weight percentage of the thickener includes, but is not limited to, 6 wt%, 12 wt%, and 20 wt%.

[0089] The weight percentage of the chelating agent includes, but is not limited to, 0.01 wt%, 0.1 wt%, and 0.2 wt%.

[0090] The skin feel modifier is present in weight percentages including, but not limited to, 0.1 wt%, 0.5 wt%, and 1 wt%.

[0091] The weight percentage of the solubilizer includes, but is not limited to, 0.5 wt%, 1 wt%, and 3 wt%.

[0092] The weight percentage of the solvent includes, but is not limited to, 42 wt%, 50 wt%, 64 wt%, and 85 wt%.

[0093] Optionally, the skin care agent is selected from one or more of shea butter, squalane, tocopheryl acetate, and behenol.

[0094] Optionally, the thickener is selected from one or more of sodium acrylate / sodium acryloyldimethyl taurate copolymer, carbomer, carboxymethyl cellulose, kaolin, and titanium dioxide.

[0095] Optionally, the chelating agent is disodium ethylenediaminetetraacetate.

[0096] Optionally, the skin feel modifier is isononyl isononanoate.

[0097] Optionally, the solubilizer is polyethylene glycol stearate.

[0098] Optionally, the solvent is water.

[0099] Optionally, the face cream may also include one or more of preservatives and fragrances.

[0100] Further, optionally, the preservative is selected from one or more of phenoxyethanol and pentanediol.

[0101] Optionally, the face cream comprises the following components in weight percentage: 0.3wt% to 18wt% of the anti-aging and whitening composition, 1wt% to 4wt% of shea butter, 0.5wt% to 3wt% of squalane, 1wt% to 4wt% of tocopheryl acetate, 2wt% to 5wt% of behenol, 1wt% to 4wt% of sodium acrylate / sodium acryloyldimethyl taurate copolymer, 1.5wt% to 5wt% of carbomer, 2wt% to 6wt% of carboxymethyl cellulose, 1wt% to 3wt% of kaolin, 0.5wt% to 2wt% of titanium dioxide, 0.01wt% to 0.2wt% of disodium ethylenediaminetetraacetate, 0.1wt% to 1wt% of isononyl isononanoate, 0.5wt% to 3wt% of polyethylene glycol stearate, and 42wt% to 85wt% of solvent.

[0102] Optionally, the face cream comprises the following components by weight percentage:

[0103] 0.375wt%–30wt% of the anti-aging and whitening liposomes, 1wt%–4wt% of shea butter, 0.5wt%–3wt% of squalane, 1wt%–4wt% of tocopheryl acetate, 2wt%–5wt% of behenol, 1wt%–4wt% of sodium acrylate / sodium acryloyldimethyl taurate copolymer, 1.5wt%–5wt% of carbomer, 2wt%–6wt% of carboxymethyl cellulose, 1wt%–3wt% of kaolin, 0.5wt%–2wt% of titanium dioxide, 0.01wt%–0.2wt% of disodium ethylenediaminetetraacetate, 0.1wt%–1wt% of isononyl isononanoate, 0.5wt%–3wt% of polyethylene glycol stearate, and 42wt%–85wt% of solvent.

[0104] The face cream of this embodiment has the advantages of the above-mentioned anti-aging and whitening combination or the above-mentioned anti-aging and whitening liposomes. The anti-aging and whitening combination or the anti-aging and whitening liposomes can help the skin care agent to more effectively play a moisturizing role.

[0105] A fourth aspect of the present invention provides a method for preparing the face cream as described above. In one embodiment, the method for preparing the face cream includes the following steps:

[0106] The solvent, a portion of the thickener, and the chelating agent are mixed to prepare a first mixture;

[0107] A second mixture is prepared by mixing the first mixture, the remaining thickener, skin care agent, skin feel modifier, and anti-aging and whitening composition or anti-aging and whitening liposomes.

[0108] Mix the second mixture with the solubilizer and emulsify.

[0109] The following description is further illustrated with specific embodiments and comparative examples. Unless otherwise specified, the raw materials involved in the following specific embodiments and comparative examples are all commercially available. Unless otherwise specified, the instruments used are all commercially available. Unless otherwise specified, the processes involved are conventionally selected by those skilled in the art.

[0110] Golden microalgae, purchased from Vedan Biotechnology Co., Ltd.

[0111] Example 1

[0112] Referring to the mass percentages in Table 1, Example 1 provides an anti-aging and whitening liposome and its preparation method, as well as a face cream and its preparation method, with the following steps:

[0113] 1) Preparation of yeast / rice fermentation product filtrate

[0114] Yeast (Saccharomyces veronae) was inoculated into sterilized activation medium and cultured at 28°C for 48 hours for later use. Under aseptic conditions, the activated inoculum was transferred to sterilized expansion medium using an inoculation loop and cultured in a constant temperature shaker at 150 rpm and 28°C for 48 hours. The expanded inoculum was then inoculated into MRS medium at an inoculation rate of 3% and cultured at 28°C for another 48 hours to obtain the yeast culture.

[0115] Wash 20g of rice with water, soak for 24 hours, drain and steam in a pot until cooked, then put into a bottle, add deionized water and let stand and cool to room temperature, while loosening the rice grains; transfer 15% of the yeast liquid (by weight of the cooked rice grains) into the cooked rice grains, and incubate at 37℃ for 2 days, then let stand, filter and collect the clear liquid to obtain the yeast / rice fermentation product filtrate.

[0116] 2) Preparation of anti-aging and skin-whitening liposomes

[0117] Dissolve 1000 mg of lecithin in 10 mL of ethanol, stir until fully dissolved, then transfer to a rotary evaporator. Under a water bath at 65 °C, allow the ethanol to evaporate slowly, forming a uniform lecithin film on the container wall.

[0118] 20 mL of phosphate buffer solution was mixed with 1164 mg of yeast / rice fermentation product filtrate, 388 mg of golden microalgae, and 776 mg of β-nicotinamide mononucleotide (NMN) and stirred until homogeneous. The mixture was then transferred to a rotary evaporator container for hydration treatment of the lecithin membrane for 20 min. After the membrane was completely detached, it was sonicated for 4 min to obtain anti-aging and whitening liposomes with a particle size of 0.1 μm to 1 μm.

[0119] 3) Preparation of the first mixture

[0120] Phase A was completely wetted and dispersed in water to obtain the first mixture.

[0121] 4) Preparation of the second mixture

[0122] Add phases B, C, and E to the first mixture in step 3), heat to 60°C, and homogenize at 3000 r / min for 3-5 minutes to ensure complete dispersion, thus obtaining the second mixture.

[0123] 5) Prepare face cream

[0124] Add phase D to the second mixture in step 4), homogenize at 3000 r / min for 3 minutes to ensure complete emulsification, and heat to 60°C to obtain the face cream.

[0125] Table 1

[0126]

[0127] Examples 2 to 3

[0128] Referring to the mass percentages in Table 2 and the preparation method of Example 1, the face creams of Examples 2 and 3 were obtained. The amounts used in step 2) of Examples 2 and 3 are as follows:

[0129] Step 2 of Example 2

[0130] Dissolve 1000 mg of lecithin in 10 mL of ethanol, stir until fully dissolved, then transfer to a rotary evaporator. Under a water bath at 65 °C, allow the ethanol to evaporate slowly, forming a uniform lecithin film on the container wall.

[0131] 40 mL of phosphate buffer solution was mixed with 2328 mg of yeast / rice fermentation product filtrate, 776 mg of golden microalgae, and 1552 mg of β-nicotinamide mononucleotide (NMN) and stirred until homogeneous. The mixture was then transferred to a rotary evaporator container for hydration treatment of the lecithin membrane for 20 min. After the membrane was completely detached, it was sonicated for 4 min to obtain anti-aging and whitening liposomes with a particle size of 0.1 μm to 1 μm.

[0132] Step 2 of Example 3

[0133] Dissolve 1000 mg of lecithin in 10 mL of ethanol, stir until fully dissolved, then transfer to a rotary evaporator. Under a water bath at 65 °C, allow the ethanol to evaporate slowly, forming a uniform lecithin film on the container wall.

[0134] 6.7 mL of phosphate buffer solution was mixed with 388 mg of yeast / rice fermentation product filtrate, 155.2 mg of golden microalgae, and 232.8 mg of β-nicotinamide mononucleotide (NMN) and stirred until homogeneous. The mixture was then transferred to a rotary evaporator container for hydration treatment of the lecithin membrane for 20 min. After the membrane was completely detached, it was sonicated for 4 min to obtain anti-aging and whitening liposomes with a particle size of 0.1 μm to 1 μm.

[0135] Table 2

[0136]

[0137] Example 4 and Comparative Examples 1 to 6

[0138] Referring to the mass percentages in Table 3, Examples 4 and Comparative Examples 1 to 6 provide an anti-aging and whitening composition, a face cream, and a method for preparing the same, with the following steps:

[0139] 1) Refer to step 1 of Example 1.

[0140] 2) Preparation of the first mixture

[0141] Phase A was completely wetted and dispersed in water to obtain the first mixture.

[0142] 3) Preparation of the second mixture

[0143] Add phases B, C, and E to the first mixture in step 2), heat to 60°C, and homogenize at 3000 r / min for 3-5 minutes to ensure complete dispersion, thus obtaining the second mixture.

[0144] 4) Prepare face cream

[0145] Add phase D to the second mixture in step 3), homogenize at 3000 r / min for 3 minutes to ensure complete emulsification, and heat to 60°C to obtain the face cream.

[0146] Table 3

[0147]

[0148] test

[0149] Test Method: Under normal circumstances, adult subjects used the test sample twice daily, morning and evening, for 56 consecutive days. A total of 90 valid subjects completed the test (10 subjects per example / comparative example), healthy Chinese women with sensitive skin, aged 28 to 50 years, with a mean age of 38.55 ± 6.44, meeting the subject voluntary inclusion and exclusion criteria. Instrumental testing was conducted before product use (D0), 7 days after product use (D7), 28 days after product use (D28), and 56 days after product use (D56).

[0150] Project 1: NMN Content Detection

[0151] The face creams prepared in Examples 1 to 4 were placed in sealed containers at room temperature. After day 0 and 12 months, the remaining content of NMN in each sample was detected by high performance liquid chromatography (HPLC), and the percentage of remaining content was calculated. The results are recorded in Table 4, where the percentage of remaining content = remaining amount / initial amount × 100%.

[0152] Table 4

[0153]

[0154] As shown in Table 4, the remaining percentage of NMN in Example 4 after 12 months was only 72.5%, indicating a significant decrease in effective content. The NMN content in the other groups did not decrease significantly, indicating that the liposome encapsulation technology did indeed play a protective role for NMN. The differences between Example 4 and Examples 1-3, combined with the data results below, further prove that the anti-aging effect of the product is related to the stable presence of NMN.

[0155] Project Two: Whitening Related

[0156] Skin color was measured using a CHROMA METER CR-400 skin color meter after 0, 7, 28, and 56 days of product use, yielding L* and ITA (Intense Powder Acquisition) values ​​(°). The rate of change of L* and ITA values ​​(°) relative to day 0 was calculated for 7, 28, and 56 days. The rate of change is calculated as follows: Rate of change = (Measurement after product use - Measurement before product use) ÷ Measurement before product use × 100%. The rate of change of L* values ​​is shown in Table 5, and the rate of change of ITA values ​​(°) is shown in Table 6. The L* value represents the skin's brightness; a higher value indicates a whiter color. Similarly, a higher ITA value indicates brighter skin, and vice versa.

[0157] Table 5

[0158]

[0159] Table 6

[0160]

[0161] 2. Use a Glossymeter GL200 to measure skin gloss after 0 days, 7 days, 28 days, and 56 days of use, obtaining gloss measurement values. Calculate the rate of change in gloss compared to day 0 for 7 days, 28 days, and 56 days. The rate of change is calculated as follows: Rate of change = (Measurement value after product use - Measurement value before product use) ÷ Measurement value before product use × 100%. The rate of change in gloss is shown in Table 7. A higher gloss measurement value indicates an increase in skin gloss.

[0162] Table 7

[0163]

[0164] As shown in Tables 5-7, compared with before use, after 7, 28, and 56 days of use of Example 1, the skin color L* value, ITA° value, and skin radiance all increased significantly. The increase in skin color and radiance was even greater in Example 2 than in Example 1, while the increase in skin color and radiance was less in Example 3 than in Example 1. This indicates that within this range, the whitening effect of the composition increases with increasing concentration. It can be inferred that the whitening effect of the face cream products in these examples is dependent on the composition. The active ingredient in Example 4 was not encapsulated with liposomes, and its effect was weaker than that of Example 1 at the same initial concentration. It can be inferred that liposome encapsulation technology also helps the active ingredient to be absorbed by the skin, thus exerting a whitening and brightening effect.

[0165] The increase in skin color and radiance after using the face creams from Comparative Examples 1 to 6 was not as significant as that in Example 4. Example 4, containing yeast / rice ferment filtrate, golden microalgae, and nicotinamide mononucleotide (NMN), showed better results, while Comparative Examples 1 to 6 contained only two or one of these three components. Therefore, this demonstrates that yeast / rice ferment filtrate containing adenosine, golden microalgae, and nicotinamide mononucleotide (NMN) has a synergistic effect.

[0166] Project 3 Anti-wrinkle related

[0167] 1. Using Primos CR to acquire and analyze facial images of skin wrinkles, the area of ​​skin wrinkles (mm²) after 0 days, 7 days, 28 days, and 56 days of use was determined. 2(Nasolabial fold) analysis value and mean skin wrinkle depth (nasolabial fold) analysis value. Calculate the skin wrinkle area (mm²) at 7 days, 28 days, and 56 days relative to day 0. 2 The rate of change of the nasolabial fold (Nasolabial fold) analysis value and the rate of change of the average depth of skin wrinkles (nasolabial fold) analysis value. Wherein, the rate of change = (measurement after product use - measurement before product use) ÷ measurement before product use × 100%. Skin wrinkle area (mm²) 2 The rate of change of (nasolabial fold) analysis values ​​is shown in Table 8. Skin wrinkle area (mm²) 2 A decrease in the nasolabial fold (Nasolabial Fold) analysis value indicates an improvement in skin wrinkles. The rate of change in the average depth of skin wrinkles (nasolabial fold) analysis value is shown in Table 9. A decrease in the average depth of skin wrinkles (nasolabial fold) analysis value indicates an improvement in skin wrinkles.

[0168] Table 8 Table 9

[0169]

[0170] 2. Use a skin microscope VC20plus image acquisition and skin surface analysis were used to obtain skin wrinkle SEw analysis values ​​after 0 days, 7 days, 28 days, and 56 days of use. The rate of change of skin wrinkle SEw analysis values ​​relative to 0 days was calculated for 7 days, 28 days, and 56 days. The rate of change is calculated as follows: Rate of change = (Measurement value after product use - Measurement value before product use) ÷ Measurement value before product use × 100%. The rate of change of skin wrinkle SEw analysis values ​​is shown in Table 10. A decrease in skin wrinkle SEw analysis values ​​indicates improvement in skin wrinkles.

[0171] Table 10

[0172]

[0173] 3. Use a skin elasticity meter The dual MPA580 was used to measure skin elasticity after 0, 7, 28, and 56 days of use, obtaining the skin elasticity R7 value. The rate of change of the skin elasticity R7 value relative to 0 days was calculated for 7, 28, and 56 days. The rate of change is calculated as follows: Rate of change = (Measurement after product use - Measurement before product use) ÷ Measurement before product use × 100%. The rate of change of skin elasticity R7 value is shown in Table 11. A higher skin elasticity R7 value indicates improved skin elasticity.

[0174] Table 11

[0175]

[0176] As shown in Tables 8-11, compared with before use, after 7 and 28 days of using Example 1, the area of ​​skin wrinkles (mm2) (nasolabial folds) and the SEw analysis value of skin wrinkles both decreased significantly, while the R7 value of skin elasticity increased significantly. The average depth of skin wrinkles (nasolabial folds) did not increase significantly by 0.11% after 7 days of use, did not increase significantly by 0.14% after 28 days of use, and decreased significantly by 2.26% after 56 days of use. A significant difference was calculated as p < 0.05. This indicates that the face cream of Example 1 can effectively exert an anti-aging effect, and this effect can be sustained with prolonged use. The rate of change of each indicator for the face cream of Example 2 was generally greater than that of Example 1, while the rate of change of each indicator for the face cream of Example 3 was generally lower than that of Example 1. This indicates that within this range, the anti-aging effect of the composition increases with increasing concentration of the anti-aging and whitening composition. It can be inferred that the anti-aging effect of the face cream products in these examples is dependent on the composition. The rate of change of each indicator in Example 4, which uses the same formula as Example 1 but without liposome encapsulation, is generally lower than that in Example 1. It can be inferred that liposome encapsulation technology effectively protects NMN, improves its stability and permeability, and that the anti-aging effect of this product is dependent on the use of liposome encapsulation technology.

[0177] The changes in all indicators in each group after using the face creams from Comparative Examples 1 to 6 were less pronounced than in Example 4. The examples containing yeast / rice ferment filtrate, golden microalgae, and nicotinamide mononucleotide (NMN) showed better anti-aging effects, while Comparative Examples 1 to 6 contained only two or one of these three components. Therefore, this demonstrates that yeast / rice ferment filtrate containing adenosine, golden microalgae, and nicotinamide mononucleotide (NMN) have a synergistic effect.

[0178] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0179] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. An anti-aging and whitening composition, characterized in that, The components include the following parts by weight: 0.1 to 10 parts yeast / rice fermentation product filtrate, 0.1 to 5 parts golden microalgae, and 0.1 to 3 parts β-nicotinamide mononucleotide; The preparation method of the yeast / rice fermentation product filtrate includes the following steps: Cooked rice is inoculated with yeast culture and fermented to prepare the yeast / rice fermentation product filtrate. The yeast strain in the yeast solution is Saccharomyces veronae. The fermentation culture temperature is 35℃~40℃.

2. The anti-aging and whitening composition according to claim 1, characterized in that, The components include the following parts by weight: 0.1 to 5 parts yeast / rice fermentation product filtrate, 0.1 to 3 parts golden microalgae, and 0.1 to 3 parts β-nicotinamide mononucleotide.

3. The anti-aging and whitening composition according to claim 1, characterized in that, The fermentation culture time is 24h~72h.

4. An anti-aging and skin-whitening liposome, characterized in that, It includes a lipid encapsulation layer and an active ingredient located within the lipid encapsulation layer, wherein the raw material of the lipid encapsulation layer includes phospholipids, and the active ingredient includes the anti-aging and whitening composition according to any one of claims 1 to 3.

5. The anti-aging and whitening liposome according to claim 4, characterized in that, The anti-aging and whitening composition accounts for 60 wt% to 80% of the total mass of the anti-aging and whitening liposomes.

6. The anti-aging and whitening liposome according to claim 4, characterized in that, The particle size of the anti-aging and whitening liposomes is 0.1μm~1μm.

7. A face cream, characterized in that, Includes the following components by weight percentage: 0.3wt%~18wt% of the anti-aging and whitening composition according to any one of claims 1 to 3 or 0.375wt%~30wt% of the anti-aging and whitening liposomes according to any one of claims 4 to 6, 4.5wt%~16wt% of skin care agent, 6wt%~20wt% of thickener, 0.01wt%~0.2wt% of chelating agent, 0.1wt%~1wt% of skin feel modifier, 0.5wt%~3wt% of solubilizer and 42wt%~85wt% of solvent.

8. A face cream, characterized in that, The composition comprises the following components by weight percentage: 0.3wt%~18wt% of the anti-aging and whitening composition according to any one of claims 1 to 3, 1wt%~4wt% of shea butter, 0.5wt%~3wt% of squalane, 1wt%~4wt% of tocopheryl acetate, 2wt%~5wt% of behenol, 1wt%~4wt% of sodium acrylate / sodium acryloyl dimethyl taurate copolymer, 1.5wt%~5wt% of carbomer, 2wt%~6wt% of carboxymethyl cellulose, 1wt%~3wt% of kaolin, 0.5wt%~2wt% of titanium dioxide, 0.01wt%~0.2wt% of disodium ethylenediaminetetraacetate, 0.1wt%~1wt% of isononyl isononanoate, 0.5wt%~3wt% of polyethylene glycol stearate, and 42wt%~85wt% of solvent.

9. A face cream, characterized in that, Includes the following components by weight percentage: 0.375wt%~30wt% of the anti-aging and whitening liposomes according to any one of claims 4 to 6, 1wt%~4wt% of shea butter, 0.5wt%~3wt% of squalane, 1wt%~4wt% of tocopheryl acetate, 2wt%~5wt% of behenol, 1wt%~4wt% of sodium acrylate / sodium acryloyl dimethyl taurate copolymer, 1.5wt%~5wt% of carbomer, 2wt%~6wt% of carboxymethyl cellulose, 1wt%~3wt% of kaolin, 0.5wt%~2wt% of titanium dioxide, 0.01wt%~0.2wt% of disodium ethylenediaminetetraacetate, 0.1wt%~1wt% of isononyl isononanoate, 0.5wt%~3wt% of polyethylene glycol stearate and 42wt%~85wt% of solvent.

10. A method for preparing a face cream according to claim 7, characterized in that, Includes the following steps: The solvent, a portion of the thickener, and the chelating agent are mixed to prepare a first mixture; A second mixture is prepared by mixing the first mixture, the remaining thickener, skin care agent, skin feel modifier, and anti-aging and whitening composition or anti-aging and whitening liposomes. Mix the second mixture with the solubilizer and emulsify.

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