Molecular instant detection device and equipment

Abstract

The present invention discloses a molecular instant detection device, which belongs to the field of medical device technology and includes a housing assembly and a detection assembly. The housing assembly includes a shell and a base. The shell and the base are detachably fixedly connected and form a receiving cavity for accommodating the detection assembly therebetween. The shell is provided with a detection hole for installing a detection tube, and the detection hole is connected to the receiving cavity. The detection assembly includes an LED light source, a color recognition sensor, and a light guide column. The LED light source, the color recognition sensor, and the light guide column are all located in the receiving cavity. The LED light source is transmitted through the light guide column to illuminate the detection tube, and the light passing through the detection tube is transmitted through the light guide column to illuminate the color recognition sensor, thereby realizing color recognition detection. The present invention combines constant temperature direct amplification technology with color recognition technology, realizing the electronic determination of medical results. At the same time, the overall structure is compact, small and portable, and the operation is simple and easy to use. It can complete the amplification and accurate detection operations of trace nucleic acid samples.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a molecular instant detection device and equipment comprising the molecular instant detection device. Background Art

[0002] Molecular POCT (point-of-care testing) refers to a rapid nucleic acid-based diagnosis performed immediately at the sampling site, eliminating the time required for sample delivery and transportation. It does not require specific laboratory conditions or professional testing personnel, and uses portable analytical instruments and supporting reagents to quickly obtain test results.

[0003] With the rapid integration and development of multiple disciplines such as medical diagnosis and molecular biology, the huge demand for molecular POCT testing technology in many fields such as infectious diseases, genetic diseases, viral respiratory infections, and tumors has led to the rapid development of this technology. Miniaturized, automated, and high-speed testing methods are being created and implemented one by one. The number of complete testing items is increasing, the quality is constantly improving, and the equipment is constantly being updated and improved.

[0004] Color-recognition-based point-of-care testing is a new technology that has emerged in recent years. It is particularly suitable for immediate on-site testing scenarios, such as in small medical institutions. This method has low equipment requirements and rapid detection. Currently, there are two main approaches. One is to directly visually observe the results of the sample solution after constant temperature amplification. This method is simple, but manual observation increases the number of human steps and the results are not conducive to the electronicization of medical results. The other approach uses a color recognition sensor to perform color recognition and automatically determine the positive or negative value of the product. However, these devices currently typically install a light shield on the detection module to ensure close coordination with the detection module to ensure effective detection. However, since part of the detection module is exposed outside the light shield, it is easy to damage the detection module when picking up or moving the device, affecting subsequent test results and resulting in poor portability of such devices. Summary of the Invention

[0005] In order to overcome the shortcomings of the existing technology, the purpose of the present invention is to provide a molecular instant detection device. By combining constant temperature direct amplification technology with color recognition technology, it can complete the amplification and accurate detection operations of trace nucleic acid samples, while making it convenient to pick up and move the detection device, and the detection device is highly portable.

[0006] The purpose of the present invention is achieved by adopting the following technical solutions:

[0007] A molecular instant detection device includes a shell assembly and a detection assembly. The shell assembly includes an outer shell and a base. The outer shell and the base are detachably fixedly connected and a accommodating cavity for accommodating the detection assembly is formed therebetween. The outer shell is provided with a detection hole for installing a detection tube, and the detection hole is connected to the accommodating cavity. The detection assembly includes an LED light source, a color recognition sensor and a light guide column. The LED light source, the color recognition sensor and the light guide column are all located in the accommodating cavity. The LED light source is transmitted through the light guide column to illuminate the detection tube. The light passing through the detection tube is transmitted through the light guide column to illuminate the color recognition sensor, thereby realizing color recognition detection.

[0008] Furthermore, the shell assembly also includes a fixing buckle, which is installed on the base. A fixing buckle groove is provided on the bottom of the shell, and the fixing buckle groove is snap-fitted with the fixing buckle.

[0009] Furthermore, the housing assembly further includes a flip cover structure, which includes a cover body, and the cover body is installed above the shell to cover the detection hole.

[0010] Furthermore, the flip cover structure further includes a rotating shaft and a torsion spring. The cover body is rotatably connected to the shell via the rotating shaft. One end of the torsion spring is mounted on the rotating shaft, and the other end is fixed inside the shell.

[0011] Furthermore, the shell assembly has a gourd-shaped appearance.

[0012] Furthermore, the molecular instant detection device also includes a control component, which includes a control board and a female connector, and the female connector is installed on the control board. The detection component also includes a pin header, and the pin header is arranged under the circuit substrate, and the pin header is plugged into the female connector.

[0013] Furthermore, the detection component also includes a circuit substrate, which is located in the accommodating cavity, and the LED light source and the color recognition sensor are respectively located on both sides of the circuit substrate.

[0014] Furthermore, the detection component also includes a metal heat-conducting block, which is installed on the circuit substrate. The metal heat-conducting block is provided with a light guide column groove. One end of the light guide column is fixed on the circuit substrate, and the other end is embedded in the light guide column groove.

[0015] Furthermore, the circuit substrate is provided with a mounting port, the metal heat-conducting block includes an upper heat-conducting block and a lower heat-conducting block, the upper heat-conducting block is snap-fitted into the mounting port, the upper heat-conducting block is provided with a mounting groove and two light-guide column grooves, the mounting groove is located between the two light-guide column grooves, the extension direction of the detection hole is in the same straight line as the extension direction of the mounting groove, the lower heat-conducting block includes two mounting blocks, the two mounting blocks are located on both sides of the upper heat-conducting block and below the circuit substrate.

[0016] The present invention also provides a molecular instant detection device, including a detection tube and any one of the above-mentioned molecular instant detection devices. The detection tube is installed in the detection hole, and the LED light source is transmitted through the light guide column to illuminate the detection tube. The light passing through the detection tube is transmitted through the light guide column to illuminate the color recognition sensor, thereby realizing color recognition detection.

[0017] Compared with the existing technology, the instant molecular detection device of the present invention has the following beneficial effects:

[0018] (1) The molecular instant detection device of the present invention includes a shell component and a detection component. The shell component includes a housing and a base. The housing and the base are detachably fixedly connected and a receiving cavity for accommodating the detection component is formed therebetween. The housing is provided with a detection hole for installing a detection tube. The detection hole is connected to the receiving cavity. The detection component includes an LED light source, a color recognition sensor, and a light guide column. The LED light source, the color recognition sensor, and the light guide column are all located in the receiving cavity. The LED light source is transmitted through the light guide column to illuminate the detection tube. The light passing through the detection tube is transmitted through the light guide column to illuminate the color recognition sensor, thereby realizing color recognition detection. The present invention combines constant temperature direct expansion technology with color recognition technology, realizing the electronic determination of medical results. At the same time, the overall structure is compact, small and portable, and the operation is simple and easy to use. It can complete the amplification and accurate detection operations of trace nucleic acid samples.

[0019] (2) The present invention realizes separated molecular detection by detachably mounting the detection tube on the detection device, which enables the detection device to be reused multiple times. It is suitable for immediate detection at home, primary health institutions, etc., reducing the cost of equipment use and saving resources.

[0020] (3) The present invention is provided with a metal heat-conducting block and a light-guiding column, so that the LED light source is transmitted through the light-guiding column to illuminate the detection tube, and the light passing through the detection tube is transmitted through the light-guiding column to illuminate the color recognition sensor. The light source is projected horizontally onto the detection tube to avoid the reflection problem caused by vertical illumination of the light source, thereby improving the sensitivity and accuracy of the detection, and is also more conducive to the subsequent development of the entire device towards fully automated operation.

[0021] (4) The present invention adopts a dual-channel, two-detection mode design, which can realize control detection of the same sample at the same time, and can also realize simultaneous detection of two samples to increase the detection speed. This device has the advantages of both accurate and efficient detection.

[0022] (5) The detection results of the present invention are displayed by the color and luminous state of the indicator light, and the light spot emitted by the indicator light is converted into a strip of light using a light guide bar. The result observation is simpler and more intuitive, and the overall shape of the device is more beautiful.

[0023] (6) The present invention is designed to use a standard 1.5 mL EP tube for sample injection and detection. It is commonly used in the market and can be used directly without the need for special accessories and consumables. It has very superior adaptability and can also greatly reduce the cost of using the device.

[0024] (7) The present invention uses EP tubes to package samples for direct testing. The entire testing process does not require opening the cover and there is no leakage, which effectively avoids problems such as aerosol contamination that may occur during the molecular testing process. The samples after testing can be directly sealed and processed, and the entire testing process effectively avoids contamination problems.

[0025] (8) The detection tube of the present invention only needs to contain a trace amount of sample of about 25uL to complete the corresponding detection operation and obtain a relatively accurate detection result, which greatly reduces the required detection sample volume and the consumption of related reagents, saving resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 A perspective view of the instant molecular detection device of the present invention;

[0027] Figure 2 for Figure 1 An exploded view of a molecular point-of-care testing device;

[0028] Figure 3 for Figure 1 A three-dimensional cutaway view of a molecular point-of-care testing device;

[0029] Figure 4 Schematic diagram of the bottom structure of the housing of the molecular instant detection device of the present invention;

[0030] Figure 5 An exploded view of the detection component of the molecular instant detection device of the present invention;

[0031] Figure 6 for Figure 5 A schematic diagram of the partial structure of the back of the detection component of the molecular instant detection device;

[0032] Figure 7 for Figure 5Schematic diagram of the structure of the metal heat-conducting block of the molecular instant detection device;

[0033] Figure 8 for Figure 5 A schematic structural diagram of the first light guide column of the molecular instant detection device;

[0034] Figure 9 Schematic diagram of the structure of the control component of the molecular instant detection device of the present invention.

[0035] In the figure: 10, housing assembly; 11, housing; 111, accommodating cavity; 112, detection hole; 113, fixing buckle groove; 114, light guide groove; 115, threaded hole; 12, base; 13, fixing buckle; 14, support block; 15, flip cover structure; 151, cover; 1511, groove; 152, rotating shaft; 153, torsion spring; 154, magnet block; 20, detection assembly; 21, LED light source; 22, color recognition sensor; 23, light guide column; 231, first light guide column; 2311, input and output terminal; 2312, refraction block; 2313 , limiting column; 232, second light guide column; 24, circuit substrate; 241, mounting port; 242, limiting hole; 25, metal heat conductive block; 251, upper part of heat conductive block; 2511, mounting groove; 2512, light guide column groove; 2513, light-proof groove; 2514, temperature measuring groove; 252, lower part of heat conductive block; 2521, mounting block; 26, electric heating film; 27, pin header; 28, button; 29, temperature sensor; 30, control component; 31, control board; 32, female header; 33, indicator light; 34, light guide strip; 35, interface; 40, detection tube. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may be another intermediate component through which it is fixed. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may be another intermediate component at the same time. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be another intermediate component at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used in this specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0039] Figures 1-9 The molecular instant detection device and equipment of the present invention includes a housing component 10 , a detection component 20 , a control component 30 and a test tube 40 .

[0040] In this embodiment:

[0041] The housing assembly 10 includes a shell 11 , a base 12 , a fixing buckle 13 , a support block 14 , and a flip cover structure 15 .

[0042] like Figure 1 、 Figure 3-4 As shown, the housing 11 is provided with a receiving cavity 111, a detection hole 112, a fixing snap groove 113, a light guide groove 114, and a threaded hole 115. In this embodiment, the interior of the housing 11 is hollowed out and the surface is coated with a black light-absorbing layer to prevent light reflection from the housing 11 during the detection process, thereby ensuring the recognition accuracy, precision, and stability of the detection results. In addition, the control component 30 and the detection component 20 can be directly installed and fixed from the bottom of the housing 11, and then assembled with the base 12 and the flip cover structure 15 to form the external shell structure. The appearance of the housing 11 is an integrated "gourd-shaped" structure, which is compact and beautiful.

[0043] The detection hole 112 is connected to the accommodating cavity 111 and is used to install the detection tube 40. In this embodiment, the cross-section of the detection hole 112 is conical, which maintains a close fit with the inserted detection tube 40 to prevent the detection tube 40 from shaking during the detection process and affecting the detection results. In addition, after being inserted into the detection hole 112, the detection tube 40 slightly protrudes from the upper end surface of the housing 11 by a certain distance, preferably 2.5 mm, to facilitate the removal of the detection tube 40 from the detection hole 112 after the test is completed.

[0044] The fixing buckle slot 113 is located at the bottom of the housing 11 and is used for engaging with the fixing buckle 13 .

[0045] Light guide slot 114 is located on the outer edge of housing 11 and communicates with cavity 111. It is used to mount light guide strip 34 in detection assembly 20, allowing operators to observe the color and brightness of indicator light 33 from outside the detection device. In this embodiment, light guide slot 114 is annular.

[0046] The threaded hole 115 is set at the bottom of the shell 11. When the double-layer structure with a small upper layer and a large lower layer composed of the control component 30 and the detection component 20 is directly inserted into the accommodating cavity 111 from the bottom of the shell 11, it can be fixed by matching with the hole on the control component 30 through the threaded hole 115, and the entire detection structure is completely encapsulated inside the shell 11.

[0047] The base 12 is detachably fixedly connected to the housing 11, and a receiving cavity 111 for accommodating the detection component 20 and the control component 30 is formed therebetween. The base 12 is used to support the control component 30 and the detection component 20. A circular plane is left at the bottom of the base 12, and the diameter is preferably 20 mm to ensure that the entire device can remain level when placed for detection, to ensure that the sample in the detection tube 40 does not tilt, and to ensure the accuracy of the detection structure.

[0048] The fixing buckle 13 is provided on the base 12 , and the fixing buckle groove 113 is engaged with the fixing buckle 13 to ensure that the base 12 and the shell 11 are firmly connected, so that the housing assembly 10 of the detection device has a basically integrated appearance.

[0049] The support block 14 is disposed on the base 12, and the control assembly 30 is mounted on the support block 14. When the outer shell of the detection device is installed, the support block 14 is in close contact with the bottom of the control assembly 30, and the support ensures that the control assembly 30 fits tightly with the outer shell 11 without any shaking gaps. In this embodiment, the dual cooperation of the threaded hole 115 and the support block 14 ensures the assembly tightness of the entire detection device, providing a certain guarantee for the detection results. The base 12, fixing clip 13, and support block 14 are integrally formed, which is relatively easy to manufacture and has a low production cost.

[0050] The flip cover structure 15 includes a cover body 151 , a rotating shaft 152 , a torsion spring 153 , and a magnet block 154 .

[0051] The cover 151 is mounted on the housing 11 and has a groove 1511 formed therein for engaging with the protruding portion of the detection tube 40. This prevents collision between the flip cover 15 and the protruding portion of the detection tube 40 when the flip cover 15 is closed, and ensures that the flip cover 15 can be properly and tightly closed. In this embodiment, the depth of the groove 1511 is 3 mm.

[0052] The cover 151 is rotatably connected to the housing 11 via a rotating shaft 152. In this embodiment, the cover 151 can freely rotate 90 degrees around the rotating shaft 152.

[0053] One end of a torsion spring 153 is mounted on the rotating shaft 152, and the other end is fixed to the interior of the housing 11. The torsion spring 153 provides mechanical assistance for opening and closing the lid, enabling the lid 151 to automatically open when the lid is opened more than 45 degrees, and automatically close when the lid is closed less than 45 degrees. In this embodiment, there are two torsion springs 153, one at each end of the rotating shaft 152.

[0054] There are at least two magnet blocks 154, one magnet block 154 mounted on the cover 151 and one magnet block 154 mounted on the top of the housing 11. The two magnet blocks 154 are arranged opposite each other. When the flip cover structure 15 is in the closed state, the two magnet blocks 154 attract each other, keeping the flip cover structure 15 tightly closed during the inspection process, preventing light leakage and automatic opening of the cover. In this embodiment, the diameter of the magnet block 154 is 1 to 5 mm and the thickness is 0.5 to 1.5 mm. Preferably, the diameter of the magnet block 154 is 3 mm and the thickness is 1 mm.

[0055] like Figure 5 As shown, the detection component 20 includes an LED light source 21, a color recognition sensor 22, a light guide column 23, a circuit substrate 24, a metal heat conductive block 25, an electric heating film 26, a pin header 27, a button 28, and a temperature sensor 29.

[0056] The circuit substrate 24 is located in the accommodating cavity 111. The circuit substrate 24 is provided with an installation opening 241 for installing the metal heat conducting block and a limiting hole 242 for installing the light guide column 23. In this embodiment, the number of the installation openings 241 is two.

[0057] The LED light source 21 is disposed on the circuit substrate 24 and is in communication with the control assembly 30 to provide illumination and fill light. In this embodiment, the LED light source 21 is an LED chip lamp bead. Preferably, the LED light source 21 is a white LED lamp bead.

[0058] like Figure 7As shown, the metal heat-conducting block 25 includes an upper heat-conducting block portion 251 and a lower heat-conducting block portion 252. The upper heat-conducting block portion 251 is a T-shaped block structure. The upper heat-conducting block portion 251 is snap-fitted into the mounting opening 241. The upper heat-conducting block portion 251 is provided with a mounting groove 2511 and two light-guide column grooves 2512. The mounting groove 2511 is located between the two light-guide column grooves 2512. The extension direction of the detection hole 112 is in the same straight line as the extension direction of the mounting groove 2511. The detection tube 40 is snap-fitted into the mounting groove 2511 through the detection hole 112; the lower heat-conducting block portion 252 includes two mounting blocks 2521. The two mounting blocks 2521 are located on both sides of the upper heat-conducting block portion 251 and below the circuit substrate 24. Specifically, the mounting blocks 2521 are distributed perpendicularly to the T-shaped block. The length of the mounting block 2521 is greater than the width of the T-shaped block. The length of the mounting block 2521 can be determined according to the number of detection tubes 40. In this embodiment, the number of upper heat-conducting block portions 251 is two, and the metal heat-conducting block 25 is integrally formed. This means that the detection device employs a dual-channel design. The interior of the housing 11 is hollowed out to conform to the appearance of the control component 30 and the detection component 20, ensuring a tight fit and a sealed seal. This completely separates the two detection channels on the metal heat-conducting block 25, preventing light leakage and interference between the two channels during simultaneous detection, thereby ensuring the accuracy of dual-channel detection. The metal heat-conducting block 25 is anodized to a matte black finish to prevent light reflected from the metal surface from influencing the detection results. The metal heat-conducting block 25 is machined from an aluminum alloy.

[0059] In a preferred embodiment, the metal heat-conducting block 25 is further provided with a light-proof groove 2513, which is located between the mounting groove 2511 and the light-guiding column groove 2512, and is used to narrow the range of incident light and received light to the central area of the detection tube 40, thereby avoiding the influence of light leakage on both sides on the detection. This is because the detection tube 40 is arc-shaped, and the light from the first light-guiding column 231 directly shines on the detection tube 40. Light will leak directly from both sides of the detection tube 40 and directly enter the second light-guiding column 232 at the other end, and be received by the color recognition sensor 22, causing errors. Specifically, the diameter of the light-proof groove 2513 is 1 to 2.5 mm. Preferably, the diameter of the light-proof groove 2513 is 2 mm.

[0060] In another preferred embodiment, Figure 6 、 Figure 7 As shown, the metal heat-conducting block 25 is also provided with a temperature measuring groove 2514, and a temperature sensor 29 is provided on the circuit substrate 24. The temperature sensor 29 is located on the back of the circuit substrate 24, and the metal heat-conducting block 25 is fixed to the circuit substrate 24 by bolts. That is, when the lower part 252 of the heat-conducting block is tightly fitted with the circuit substrate 24, the temperature sensor 29 is completely embedded in the temperature measuring groove 2514, with a compact structure and ingenious layout.

[0061] The light guide 23 is mounted on the metal heat-conducting block 25 and is used to transmit light from the LED light source 21 to the detection tube 40. The light that passes through the detection tube 40 is then transmitted to the color recognition sensor 22 for subsequent color recognition detection. In this embodiment, the light guide 23 is a right-angle light guide.

[0062] The light guide 23 includes a first light guide 231 and a second light guide 232. The first light guide 231 is positioned above the LED light source 21 and converts the vertically upward light emitted by the LED light source 21 by 90 degrees into horizontal light. The second light guide 232 is positioned above the color recognition sensor 22 and converts the horizontal light transmitted through the detection tube 40 by 90 degrees into vertically downward light. Specifically, the first light guide 231 and the second light guide 232 are symmetrically arranged on opposite sides of the metal heat conductive block 25. Specifically, one end of the first light guide 231 is fixed to the circuit substrate 24, and the other end is embedded in one light guide groove 2512. The second light guide 232 has one end fixed to the circuit substrate 24, and the other end is embedded in another light guide groove 2512. In this embodiment, the light guide 23 is made of flame-retardant engineering plastic, and the diameter of the light guide surface is preferably 3.81 mm, which effectively prevents deformation of the light guide 23 due to the temperature of the metal heat conductive block 25.

[0063] like Figure 8 As shown, the first light guide column 231 includes an input and output end 2311, a refractive block 2312 and a limiting column 2313. The input and output end 2311 is engaged with the light guide column groove 2512, the side of the refractive block 2312 is fitted with the side of the metal heat conductive block 25, and the limiting column 2313 is plugged into the limiting hole 242 on the circuit substrate 24, so that the overall structure of the detection component 20 is compact and stable.

[0064] The electric heating film 26 is attached to the bottom of the metal heat conductive block 25, substantially completely covering the entire bottom of the metal heat conductive block 25. The electric heating film 26 heats the metal heat conductive block 25, achieving uniform heating of the detection tube 40 by the metal heat conductive block 25. In this embodiment, the electric heating film 26 preferably has a size of 15×10 mm. After actual testing, the power of the electric heating film 26 is preferably 1W. The heating temperature of the electric heating film 26 is actively adjusted by the control board 31 to maintain the metal heat conductive block 25 at the constant temperature required for constant-temperature nucleic acid amplification.

[0065] The color recognition sensor 22 is arranged on the circuit substrate 24 and is in communication connection with the control component 30. When the constant temperature amplification process is completed, the sample liquid in the detection tube 40 changes color. If it is positive, the sample liquid turns yellow-green; if it is negative, the sample liquid turns purple. The color recognition sensor 22 converts the recognized color light into a digital signal and transmits it to the control board 31 for comparison with the standard value to determine whether the sample in the detection tube 40 is positive or negative. The control board 31 indicates the recognition result by controlling the color of the indicator light 33, thereby avoiding direct contact and contamination of the detection sample. It has the advantages of high precision, high sensitivity and stability, easy operation, etc., and is easy to develop in the direction of automation.

[0066] In this embodiment, the number of LED light sources 21 , the number of color recognition sensors 22 , and the number of light guides 23 correspond to the number of detection tubes 40 .

[0067] The pin headers 27 are provided on and below the circuit substrate 24 and are used for plugging and mating with the female connectors 32 on the control assembly 30. In this embodiment, there are two pin headers 27.

[0068] A button 28 is provided on the circuit board 24 and is used to control the operation and testing of the detection device. After the device is powered on, long-press button 28 until the indicator light 33 changes from a short green light to a flashing blue state, indicating that the device has been turned on and entered the heating state. When preparing to perform a test, press button 28 to automatically test the sample. The detection device has a dual-channel design. When performing automatic identification testing, pressing the control button 28 on the control board 31 only once puts the device into single-test mode, i.e., testing one channel. Pressing the control button 28 twice in short succession puts the device into dual-test mode, i.e., testing both channels simultaneously. This can be used to test two samples simultaneously, or to test the same sample twice.

[0069] like Figure 9 As shown, the control assembly 30 includes a control board 31 , a female connector 32 , an indicator light 33 , a light guide bar 34 , and an interface 35 .

[0070] The control board 31 is arranged in the accommodating cavity 111 and is located below the circuit substrate 24. It is used to accurately adjust the temperature of the metal heat conductive block 25 to perform constant temperature amplification operation. As a simplified nucleic acid amplification method, the nucleic acid constant temperature direct amplification technology can directly use the original sample for amplification without the need for tedious sample processing steps, which can effectively improve the detection efficiency.

[0071] The female header 32 is installed on the control board 31, and the pin header 27 is plugged into the female header 32. This detachable double-layer structure with a small upper layer and a large lower layer facilitates the welding and arrangement of various electronic components, and also facilitates the assembly of various components, making the overall structure more compact and space utilization more efficient.

[0072] The indicator light 33 is provided on the control board 31 and is in communication with the control board 31. The indicator light 33 has multiple colors to display the status. The blue flashing light indicates that the device is in the heating state. The blue light is always on to indicate that the device is in the constant temperature waiting state. The red light is always on to indicate that the test result is positive. The green light is always on to indicate that the test result is negative. The green light is short to indicate that the device is turned on.

[0073] One end of the light guide bar 34 is connected to the indicator light 33, and the other end is located in the light guide groove 114. It is used to guide the color and brightness of the indicator light 33 to the outside of the housing 11. Specifically, the light guide bar 34 converts the light spot emitted by the indicator light 33 into a strip of light, making it easier for the operator to observe the color and brightness of the indicator light 33 from outside the detection device, and also improving the overall appearance of the device. In this embodiment, the cross-sectional diameter of the light guide bar 34 is preferably 3 mm.

[0074] The interface 35 is provided on the control board 31 and is used to supply power to the detection device. In this embodiment, the interface 35 is a Type-C interface.

[0075] like Figure 1-3 As shown, the present application also provides a molecular instant detection device, including the above-mentioned molecular instant detection device and a detection tube 40.

[0076] The detection tube 40 is installed in the mounting groove 2511 on the metal heat-conducting block 25 through the detection hole 112. The LED light source 21 is transmitted and irradiated onto the detection tube 40 through the light guide column 23. The light passing through the detection tube 40 is transmitted and irradiated onto the color recognition sensor 22 through the light guide column 23, thereby realizing color recognition detection. In addition, since the cross-sectional dimensions of the light guide column 23 are relatively small and the dimensions of the mounting groove 2511 reserved on the metal heat-conducting block 25 are also relatively small, the detection tube 40 only needs to be equipped with a trace sample of 20 to 30uL to complete the detection work, preferably 25uL, which greatly reduces the required detection sample volume and the consumption of related reagents, saving resources. In this embodiment, the detection tube 40 is a standard 1.5mL EP tube, which is commonly used in the market and can be used directly without the need for separately equipped with special related accessories and consumables. It has very superior adaptability and can also greatly reduce the cost of using the device.

[0077] The working process of this application is as follows:

[0078] Press and hold the button 28 until the indicator light 33 changes from short green light to flashing blue. At this time, the color and lighting state of the indicator light 33 are displayed through the light guide bar 34, indicating that the entire detection device has entered the heating state. When the indicator light 33 turns to a solid blue light state, it indicates that the metal heat conductive block 25 has reached and maintained a constant temperature.

[0079] Open the flip cover 15, insert the test tube 40 containing the sample to be tested and related reagents into the detection hole 112, close the flip cover 15, and wait for 20 to 40 minutes to ensure that the sample in the test tube 40 completes the isothermal amplification process;

[0080] After the above amplification process is completed, press the button 28, and the entire device will automatically perform identification and detection. Specifically, the control board 31 controls the LED light source 21 to work and emit light. The first light guide 231 turns the emitted light 90 degrees to transmit it to the detection tube 40. The second light guide 232 turns the light passing through the detection tube 40 90 degrees to transmit it to the color recognition sensor 22. The color recognition sensor 22 converts the recognized color digital signal and compares it with the standard value to determine whether the sample is positive. If the sample is positive, the indicator light 33 lights up red. If the sample is negative, the indicator light 33 lights up green.

[0081] After completing the above detection operation, open the flip cover structure 15, take out the detection tube 40 that has completed the detection for waste disposal, press and hold the button 28 again or directly unplug the power line at the interface 35, and the device will enter the shutdown state.

[0082] The present application combines the constant temperature direct expansion technology with the color recognition technology, realizing the electronic determination of medical results. At the same time, the overall structure is compact, small and portable, and the operation is simple and easy to use, and can complete the amplification and accurate detection of trace nucleic acid samples; through the detection tube 40 detachably mounted on the detection device, separate molecular detection is realized, and the detection device can be reused many times, which is suitable for instant detection sites such as home use and primary health institutions, reducing the cost of equipment use and saving resources; the present application is set up in the metal heat conductive block 25 and the light guide column 23, so that the LED light source 21 is transmitted to the detection tube 40 through the light guide column 23, and the light passing through the detection tube 40 is transmitted to the color through the light guide column 23. On the color recognition sensor 22, the light source is projected horizontally onto the detection tube 40 to avoid the reflection problem caused by vertical illumination of the light source, thereby improving the sensitivity and accuracy of the detection, and is also more conducive to the subsequent development of the entire device towards fully automated operation; the present application adopts a dual-channel, two-detection mode design scheme, which can realize simultaneous control detection of the same sample, and can also realize simultaneous detection of two samples to increase the detection speed. The present device has the advantages of both accurate and efficient detection; the detection results of the present application are displayed by the color and luminous state of the indicator light 33, and the light guide strip 34 is used to convert the light spot emitted by the indicator light 33 into a strip of light, so that the observation of the results is simpler and more intuitive, and the overall shape of the device is more beautiful.

[0083] The above embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patented invention. It should be noted that those skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention. These variations and improvements are equivalent modifications and improvements to the above embodiments based on the essential technology of the present invention and fall within the scope of protection of the present invention.

Claims

1. A molecular instant detection device, comprising a housing assembly, characterized in that: It also includes a detection component, the housing component includes a shell and a base, the shell and the base are detachably fixedly connected and a receiving cavity for accommodating the detection component is formed therebetween, the shell is provided with a detection hole for installing a detection tube, the detection hole is communicated with the receiving cavity, the detection component includes an LED light source, a color recognition sensor and a light guide column, the LED light source, the color recognition sensor and the light guide column are all located in the receiving cavity, the LED light source is transmitted through the light guide column to illuminate the detection tube, and the light transmitted through the detection tube is transmitted through the light guide column to illuminate the color recognition sensor, thereby realizing color recognition detection; The detection component further includes a circuit substrate, the circuit substrate is located in the accommodating cavity, and the LED light source and the color recognition sensor are respectively located on both sides of the circuit substrate; The detection assembly further includes a metal heat-conducting block, which is mounted on the circuit substrate and is provided with a light-guide column groove. One end of the light-guide column is fixed to the circuit substrate, and the other end is embedded in the light-guide column groove. The circuit substrate is provided with a mounting opening, the metal heat-conducting block includes an upper portion of the heat-conducting block and a lower portion of the heat-conducting block, the upper portion of the heat-conducting block is snap-fitted into the mounting opening, the upper portion of the heat-conducting block is provided with a mounting groove and two light guide column grooves, the mounting groove is located between the two light guide column grooves, the extension direction of the detection hole is in the same straight line as the extension direction of the mounting groove, the lower portion of the heat-conducting block includes two mounting blocks, the two mounting blocks are located on both sides of the upper portion of the heat-conducting block and below the circuit substrate; The light guide column includes a first light guide column and a second light guide column, and the first light guide column and the second light guide column are arranged on both sides of the metal heat conductive block; The first light guide column includes input and output ends, a refraction block and a limiting column. The input and output ends are embedded in the groove of the light guide column, the side of the refraction block is in contact with the side of the metal heat conductive block, and the limiting column is inserted into the limiting hole on the circuit substrate.

2. The molecular instant detection device according to claim 1, characterized in that: The shell assembly further comprises a fixing buckle, which is mounted on the base. A fixing buckle groove is provided at the bottom of the shell, and the fixing buckle groove is snap-fitted with the fixing buckle.

3. The molecular instant detection device according to claim 1, characterized in that: The housing assembly further comprises a flip cover structure, which comprises a cover body, and the cover body is installed above the shell to cover the detection hole.

4. The molecular instant detection device according to claim 3, characterized in that: The flip cover structure further includes a rotating shaft and a torsion spring. The cover body is rotatably connected to the shell via the rotating shaft. One end of the torsion spring is mounted on the rotating shaft, and the other end is fixed inside the shell.

5. The molecular instant detection device according to claim 1, characterized in that: The shell assembly has a gourd-shaped appearance.

6. The molecular instant detection device according to claim 1, characterized in that: The molecular instant detection device also includes a control component, which includes a control board and a female connector. The female connector is installed on the control board. The detection component also includes a pin header, which is arranged below the circuit substrate and is plugged into the female connector.

7. A molecular instant detection device, comprising a detection tube, characterized in that: It also includes the molecular instant detection device according to any one of claims 1 to 6, wherein the detection tube is installed in the detection hole, the LED light source is transmitted through the light guide column to illuminate the detection tube, and the light passing through the detection tube is transmitted through the light guide column to illuminate the color recognition sensor, thereby realizing color recognition detection.

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