Ecological control methods for root-knot nematode diseases

Abstract

This invention discloses an ecological control method for root-knot nematode disease, comprising the following steps: planting crops in soil mixed with earthworm castings, and periodically irrigating the crops with earthworm fermentation liquid diluted with water during crop growth. The earthworm fermentation liquid is prepared as follows: Step 1: Earthworms are taken, ground and crushed into a slurry, and placed in a fermentation reactor for fermentation. During fermentation, chitosan, humic acid, Bacillus coagulans, and agar powder are added. The weight ratio of chitosan, humic acid, Bacillus coagulans, and agar powder to earthworms is (0.3–0.5):(0.1–0.3):(0.1–0.3):(0.6–0.8):1. The fermentation temperature is 45–50℃, and the fermentation time is 7–15 days, thereby obtaining a fermentation mixture. Step 2: The fermentation mixture is initially filtered through gauze, and the filtrate is then finely filtered through a ceramic membrane tube assembly. The filtrate is collected to obtain the earthworm fermentation liquid. This invention utilizes the control effect of earthworms on soil root-knot nematodes to construct an ecological control method for root-knot nematode diseases, which has a wide range of applications.

Description

Technical Field

[0001] This invention relates to the field of pest and disease control technology. More specifically, this invention relates to an ecological control method for root-knot nematode disease. Background Technology

[0002] Root-knot nematodes are highly specialized omnivorous plant pathogenic nematodes with a wide host range, affecting more than 130 crops from 39 families. The nematodes are very small, difficult to see with the naked eye, and are mostly distributed in the 0-20 cm soil depth, especially in the 3-9 cm depth where their numbers are highest. They are dioecious; adult females are pear-shaped, while adult males are thread-like. They often exist as eggs or second-instar larvae left in the soil or manure with plant debris. They overwinter or, when conditions are suitable the following year, the second-instar larvae invade through tender roots and reproduce, causing damage. Using biological principles to control root-knot nematode diseases is currently a major research focus and development trend. For example, patent application CN109429933A discloses a method for controlling watermelon root-knot nematodes using a combination of powdered microorganisms and earthworms. Earthworms are inoculated into the soil to control root-knot nematodes during the watermelon growing stage. However, because earthworms have certain environmental requirements, requiring the soil to maintain specific temperature, humidity, and pH levels, the application of this control method is limited. Summary of the Invention

[0003] One objective of this invention is to provide an ecological control method for root-knot nematode disease, which utilizes earthworm castings and earthworm fermentation liquid for ecological control of root-knot nematodes and has a wide range of applications.

[0004] To achieve these objectives and other advantages according to the invention, according to one aspect of the invention, an ecological control method for root-knot nematode disease is provided, comprising the following steps: planting crops in soil mixed with earthworm castings, and periodically irrigating the crops with a diluted earthworm fermentation solution during crop growth, wherein the preparation process of the earthworm fermentation solution is as follows:

[0005] Step 1: Take earthworms, grind and crush them into a slurry, and place it in a fermentation reactor for fermentation. During the fermentation process, add chitosan, humic acid, Bacillus coagulans and agar powder. The weight ratio of chitosan, humic acid, Bacillus coagulans and agar powder to earthworms is (0.3-0.5):(0.1-0.3):(0.1-0.3):(0.6-0.8):1. The fermentation temperature is 45-50℃ and the fermentation time is 7-15 days, thereby obtaining a fermented mixture.

[0006] Step 2: Filter the fermentation mixture through gauze, then filter the filtrate through a ceramic membrane tube assembly, and collect the filtrate to obtain the earthworm fermentation liquid.

[0007] Preferably, the amount of earthworm castings added is 100-200 kg / mu.

[0008] Preferably, the irrigation volume of the earthworm fermentation liquid is 5-20 L / mu.

[0009] Preferably, the ceramic membrane tube group in step two is composed of two sets of ceramic membrane tubes connected in series, and each set of ceramic membrane tubes is filled with 50 to 80 ceramic membrane cores, the average pore size of the ceramic membrane cores being 100 to 500 nm.

[0010] Preferably, the method for preparing the ceramic membrane core is as follows:

[0011] Step a: Add N-methylpyrrolidone to the polyimide precursor polyamic acid, stir and mix evenly, add yttrium-stabilized zirconia ceramic powder with an average particle size of 200 nm, stir and mix evenly, add polyethersulfone again, stir and mix evenly, and degas to obtain casting solution, wherein the weight ratio of polyamic acid, N-methylpyrrolidone, yttrium-stabilized zirconia ceramic powder and polyethersulfone is (17-30):(40-60):(40-55):(17-30);

[0012] Step b: Using a mixture of N-methylpyrrolidone and water at a weight ratio of 19:1 as the core liquid, the casting liquid is extruded through a spinneret under a nitrogen pressure of 0.1 MPa and immersed in a water tank for 6 to 12 hours. The resulting wet film is then removed and dried.

[0013] Step c: Increase the temperature of the dried wet film to 500°C at a heating rate of 2°C / min and keep it at that temperature for 2 hours. Then increase the temperature to 800°C at a heating rate of 3°C / min and keep it at that temperature for 5 hours. Next, increase the temperature to 1500°C at a heating rate of 5°C / min and keep it at that temperature for 8 hours. Finally, gradually cool the film to room temperature at a cooling rate of 5°C / min to obtain the ceramic film core.

[0014] Preferably, the thickness of the ceramic membrane core is 100–200 μm.

[0015] The present invention has at least the following beneficial effects: The ecological control method for root-knot nematode disease described in this invention utilizes earthworm castings and earthworm fermentation liquid for ecological control of root-knot nematodes, and has a wide range of applications; the earthworm fermentation liquid is produced by co-fermentation of earthworms, chitosan, humic acid, Bacillus coagulans, and agar powder, which promotes the fermentation and decomposition of earthworms, effectively retains the effective components that inhibit root-knot nematodes, and improves the control effect of root-knot nematodes; the earthworm fermentation liquid is filtered using a self-made ceramic membrane tube assembly, which improves the purity of the earthworm fermentation liquid and reduces the amount used per unit area of ​​soil.

[0016] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Detailed Implementation

[0017] The present invention will now be described in further detail with reference to specific embodiments, so that those skilled in the art can implement it based on the description.

[0018] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0019] It should be noted that, unless otherwise specified, the experimental methods described in the following implementation plan are all conventional methods, and the reagents and materials described are all commercially available unless otherwise specified.

[0020] Example 1

[0021] An ecological control method for root-knot nematode disease includes the following steps: planting crops in soil mixed with earthworm castings, and periodically irrigating the crops with a diluted earthworm fermentation solution during crop growth. The amount of earthworm castings is 150 kg / mu, and the irrigation volume of the earthworm fermentation solution is 12 L / mu. The preparation process of the earthworm fermentation solution is as follows:

[0022] Step 1: Take earthworms, grind and crush them into a slurry, and place it in a fermentation reactor for fermentation. During the fermentation process, add chitosan, humic acid, Bacillus coagulans and agar powder. The weight ratio of chitosan, humic acid, Bacillus coagulans and agar powder to earthworms is 0.4:0.2:0.2:0.7:1. The fermentation temperature is 45℃ and the fermentation time is 10 days, thereby obtaining a fermented mixture.

[0023] Step 2: The fermentation mixture is initially filtered through gauze, and the filtrate is then finely filtered through a ceramic membrane tube assembly. The filtrate is collected to obtain the earthworm fermentation liquid. The ceramic membrane tube assembly consists of two sets of ceramic membrane tubes connected in series. Each set of ceramic membrane tubes is filled with 60 ceramic membrane cores. The average pore size of the ceramic membrane cores is 200 nm. The ceramic membrane tube assembly is a commercially available Sartorius hollow fiber ceramic membrane (TFF).

[0024] Example 2

[0025] An ecological control method for root-knot nematode disease, differing from Example 1 in that the ceramic membrane core is self-made, and its preparation method is as follows:

[0026] Step a: Add N-methylpyrrolidone to the polyimide precursor polyamic acid, stir and mix evenly, add yttrium-stabilized zirconia ceramic powder with an average particle size of 200 nm, stir and mix evenly, add polyethersulfone again, stir and mix evenly, and degas to obtain casting solution, wherein the weight ratio of polyamic acid, N-methylpyrrolidone, yttrium-stabilized zirconia ceramic powder and polyethersulfone is 25:50:50:20;

[0027] Step b: Using a mixture of N-methylpyrrolidone and water at a weight ratio of 19:1 as the core liquid, the casting liquid is extruded through a spinneret under a nitrogen pressure of 0.1 MPa and immersed in a water tank for 10 hours. The resulting wet film is then removed and dried.

[0028] Step c: Increase the temperature of the dried wet film to 500℃ at a heating rate of 2℃ / min and hold for 2 hours. Continue to increase the temperature to 800℃ at a heating rate of 3℃ / min and hold for 5 hours. Then increase the temperature to 1500℃ at a heating rate of 5℃ / min and hold for 8 hours. Finally, gradually cool the film to room temperature at a cooling rate of 5℃ / min to obtain a ceramic film core with a thickness of 150μm.

[0029] Example 3

[0030] An ecological control method for root-knot nematode disease includes the following steps: planting crops in soil mixed with earthworm castings, and periodically irrigating the crops with a diluted earthworm fermentation solution during crop growth. The earthworm castings are added at a rate of 100 kg / mu, and the earthworm fermentation solution is applied at a rate of 5 L / mu. The preparation process of the earthworm fermentation solution is as follows:

[0031] Step 1: Take earthworms, grind and crush them into a slurry, and place it in a fermentation reactor for fermentation. During the fermentation process, add chitosan, humic acid, Bacillus coagulans and agar powder. The weight ratio of chitosan, humic acid, Bacillus coagulans and agar powder to earthworms is 0.3:0.1:0.1:0.6:1. The fermentation temperature is 45℃ and the fermentation time is 7 days, thereby obtaining a fermentation mixture.

[0032] Step 2: The fermentation mixture is initially filtered through gauze, and the filtrate is then finely filtered through a ceramic membrane tube assembly. The filtrate is collected to obtain the earthworm fermentation liquid. The ceramic membrane tube assembly consists of two sets of ceramic membrane tubes connected in series. Each set of ceramic membrane tubes is filled with 50 ceramic membrane cores, and the average pore size of the ceramic membrane cores is 100 nm.

[0033] The method for preparing the ceramic membrane core is as follows:

[0034] Step a: Add N-methylpyrrolidone to the polyimide precursor polyamic acid, stir and mix evenly, add yttrium-stabilized zirconia ceramic powder with an average particle size of 200 nm, stir and mix evenly, add polyethersulfone again, stir and mix evenly, and degas to obtain casting solution, wherein the weight ratio of polyamic acid, N-methylpyrrolidone, yttrium-stabilized zirconia ceramic powder and polyethersulfone is 17:40:40:17;

[0035] Step b: Using a mixture of N-methylpyrrolidone and water at a weight ratio of 19:1 as the core liquid, the casting liquid is extruded through a spinneret under a nitrogen pressure of 0.1 MPa and immersed in a water tank for 6 hours. The resulting wet film is then removed and dried.

[0036] Step c: Increase the temperature of the dried wet film to 500℃ at a heating rate of 2℃ / min and hold for 2 hours. Continue to increase the temperature to 800℃ at a heating rate of 3℃ / min and hold for 5 hours. Then increase the temperature to 1500℃ at a heating rate of 5℃ / min and hold for 8 hours. Finally, gradually cool the film to room temperature at a cooling rate of 5℃ / min to obtain a ceramic film core with a thickness of 100μm.

[0037] Example 4

[0038] An ecological control method for root-knot nematode disease includes the following steps: planting crops in soil mixed with earthworm castings, and periodically irrigating the crops with a diluted earthworm fermentation solution during crop growth. The amount of earthworm castings is 200 kg / mu, and the irrigation volume of the earthworm fermentation solution is 20 L / mu. The preparation process of the earthworm fermentation solution is as follows:

[0039] Step 1: Take earthworms, grind and crush them into a slurry, and place it in a fermentation reactor for fermentation. During the fermentation process, add chitosan, humic acid, Bacillus coagulans and agar powder. The weight ratio of chitosan, humic acid, Bacillus coagulans and agar powder to earthworms is 0.5:0.3:0.3:0.8:1. The fermentation temperature is 50℃ and the fermentation time is 15 days, thereby obtaining a fermented mixture.

[0040] Step 2: The fermentation mixture is initially filtered through gauze, and the filtrate is then finely filtered through a ceramic membrane tube assembly. The filtrate is collected to obtain the earthworm fermentation liquid. The ceramic membrane tube assembly consists of two sets of ceramic membrane tubes connected in series. Each set of ceramic membrane tubes is filled with 80 ceramic membrane cores, and the average pore size of the ceramic membrane cores is 500 nm.

[0041] The method for preparing the ceramic membrane core is as follows:

[0042] Step a: Add N-methylpyrrolidone to the polyimide precursor polyamic acid, stir and mix evenly, add yttrium-stabilized zirconia ceramic powder with an average particle size of 200 nm, stir and mix evenly, add polyethersulfone again, stir and mix evenly, and degas to obtain casting solution, wherein the weight ratio of polyamic acid, N-methylpyrrolidone, yttrium-stabilized zirconia ceramic powder and polyethersulfone is 30:60:55:30;

[0043] Step b: Using a mixture of N-methylpyrrolidone and water at a weight ratio of 19:1 as the core liquid, the casting liquid is extruded through a spinneret under a nitrogen pressure of 0.1 MPa and immersed in a water tank for 12 hours. The resulting wet film is then removed and dried.

[0044] Step c: Increase the temperature of the dried wet film to 500℃ at a heating rate of 2℃ / min and hold for 2 hours. Continue to increase the temperature to 800℃ at a heating rate of 3℃ / min and hold for 5 hours. Then increase the temperature to 1500℃ at a heating rate of 5℃ / min and hold for 8 hours. Finally, gradually cool the film to room temperature at a cooling rate of 5℃ / min to obtain a ceramic film core with a thickness of 200μm.

[0045] Comparative Example 1

[0046] An ecological control method for root-knot nematode disease and its preparation method are disclosed. The difference between this method and Example 1 is that in the preparation process of the earthworm fermentation liquid, step one is as follows: earthworms are taken, ground and crushed into a slurry, and placed in a fermentation reactor for fermentation at a temperature of 45°C for 10 days, thereby obtaining a fermentation mixture.

[0047] Comparative Example 2

[0048] An ecological control method for root-knot nematode disease and its preparation method differ from Example 1 in that the first step in the preparation of the earthworm fermentation liquid is as follows: earthworms are taken, ground and crushed into a slurry, placed in a fermentation reactor for fermentation, chitosan is added during the fermentation process, the weight ratio of chitosan to earthworms is 0.4:1, the fermentation temperature is 45℃, and the fermentation time is 10 days, thereby obtaining a fermentation mixture.

[0049] Comparative Example 3

[0050] An ecological control method for root-knot nematode disease and its preparation method differ from Example 1 in that, in the preparation of the earthworm fermentation liquid, step one is as follows: earthworms are taken, ground and crushed into a slurry, and placed in a fermentation reactor for fermentation. During the fermentation process, humic acid, Bacillus coagulans, and agar powder are added. The weight ratio of humic acid, Bacillus coagulans, and agar powder to earthworms is 0.2:0.2:0.7:1. The fermentation temperature is 45℃, and the fermentation time is 10 days, thereby obtaining a fermentation mixture.

[0051] Comparative Example 4

[0052] An ecological control method for root-knot nematode disease and its preparation method differ from Example 1 in that the first step in the preparation of the earthworm fermentation liquid is as follows: earthworms are taken, ground and crushed into a slurry, placed in a fermentation reactor for fermentation, humic acid is added during the fermentation process, the weight ratio of humic acid to earthworms is 0.2:1, the fermentation temperature is 45℃, and the fermentation time is 10 days, thereby obtaining a fermentation mixture.

[0053] Comparative Example 5

[0054] An ecological control method for root-knot nematode disease and its preparation method are disclosed. The difference between this method and Example 1 is that in step two, commercially available nano-sized filter paper is used for filtration. This filter paper is made of cotton fibers.

[0055] Comparative Example 6

[0056] An ecological control method for root-knot nematode disease and its preparation method, differing from Example 2 in that the ceramic membrane core is prepared as follows:

[0057] Step a: Add N-methylpyrrolidone to the polyimide precursor polyamic acid, stir and mix evenly, add yttrium-stabilized zirconia ceramic powder with an average particle size of 200 nm, stir and mix evenly, and degas to obtain casting solution, wherein the weight ratio of polyamic acid, N-methylpyrrolidone and yttrium-stabilized zirconia ceramic powder is 25:50:50;

[0058] Step b: Using a mixture of N-methylpyrrolidone and water at a weight ratio of 19:1 as the core liquid, the casting liquid is extruded through a spinneret under a nitrogen pressure of 0.1 MPa and immersed in a water tank for 10 hours. The resulting wet film is then removed and dried.

[0059] Step c: Increase the temperature of the dried wet film to 500℃ at a heating rate of 2℃ / min and hold for 2 hours. Continue to increase the temperature to 800℃ at a heating rate of 3℃ / min and hold for 5 hours. Then increase the temperature to 1500℃ at a heating rate of 5℃ / min and hold for 8 hours. Finally, gradually cool the film to room temperature at a cooling rate of 5℃ / min to obtain a ceramic film core with a thickness of 150μm.

[0060] Comparative Example 7

[0061] An ecological control method for root-knot nematode disease and its preparation method, differing from Example 2 in that the ceramic membrane core is prepared as follows:

[0062] Step a: Add N-methylpyrrolidone to polyethersulfone, stir and mix evenly, add yttrium-stabilized zirconia ceramic powder with an average particle size of 200 nm, stir and mix evenly, and degas to obtain casting liquid, wherein the weight ratio of N-methylpyrrolidone, yttrium-stabilized zirconia ceramic powder and polyethersulfone is 50:50:20.

[0063] Step b: Using a mixture of N-methylpyrrolidone and water at a weight ratio of 19:1 as the core liquid, the casting liquid is extruded through a spinneret under a nitrogen pressure of 0.1 MPa and immersed in a water tank for 10 hours. The resulting wet film is then removed and dried.

[0064] Step c: Increase the temperature of the dried wet film to 500℃ at a heating rate of 2℃ / min and hold for 2 hours. Continue to increase the temperature to 800℃ at a heating rate of 3℃ / min and hold for 5 hours. Then increase the temperature to 1500℃ at a heating rate of 5℃ / min and hold for 8 hours. Finally, gradually cool the film to room temperature at a cooling rate of 5℃ / min to obtain a ceramic film core with a thickness of 150μm.

[0065] Test on the toxic effect of earthworm fermentation liquid on root-knot nematodes

[0066] Earthworm fermentation broths prepared in Examples 1-4 and Comparative Examples 1-7 were used to test their effect on second-instar larvae of root-knot nematodes: 10 active second-instar larvae and 150 μL of earthworm fermentation broth were added to each well of a 96-well microplate, with sterile water treatment as a control. The plates were placed in a constant temperature incubator at 26°C. The survival of nematodes in each well was observed using an optical microscope after 24, 36, and 48 hours, and the mortality rate was calculated. Mortality rate (%) = number of dead nematodes / number of tested nematodes × 100.

[0067] Earthworm fermentation broths prepared in Examples 1-4 and Comparative Examples 1-7 were used to test their effect on the hatching of root-knot nematode eggs: 40 μL of egg solution (about 100 eggs) and 200 μL of earthworm fermentation broth were added to each well of a 96-well microplate, with sterile water treatment as a control. The plates were placed in a constant temperature incubator at 26℃. After 3 days, the number of hatched nematodes and the number of unhatched eggs were observed under a microscope. The hatching rate was calculated as follows: Hatching rate (%) = Number of hatched nematodes / (Number of hatched nematodes + Number of unhatched eggs) × 100.

[0068] Earthworm fermentation broths prepared in Examples 1-4 and Comparative Examples 1-7 were used to test their effect on the hatching of root-knot nematode oocysts. Three sterilized oocysts of similar color and size were added to each well of a 96-well microplate, along with 200 μL of earthworm fermentation broth. Sterile water treatment was used as a control. The plates were incubated at 26°C for 3 days, and the number of hatched nematodes was counted. The relative inhibition rate was calculated as follows: Relative inhibition rate (%) = (Number of nematodes hatched from control oocysts - Number of nematodes hatched from treated oocysts) / Number of nematodes hatched from control oocysts × 100. The results are shown in Table 1.

[0069] Table 1

[0070]

[0071]

[0072] As shown in Table 1, the earthworm fermentation broth prepared in Examples 1-4 showed significantly better toxicity against root-knot nematodes than Comparative Examples 1-7, with Example 2 exhibiting the best toxicity. This is because the earthworm fermentation broth in Example 2 was prepared by co-fermenting earthworms with chitosan, humic acid, Bacillus coagulans, and agar powder, which promoted the fermentation and decomposition of earthworms, effectively preserving the active ingredients that inhibit root-knot nematodes and improving the control effect against them. In addition, the self-made ceramic membrane core used had the highest retention rate and the best purity of the active ingredients in the earthworm fermentation broth. In Comparative Examples 1-4, different additives were added during the earthworm fermentation process, resulting in poor fermentation effects, low content of active ingredients, and poor control of root-knot nematodes. In Comparative Example 5, ordinary commercially available filter paper was used for filtration, resulting in poor filtration effects, low purity of active ingredients in the earthworm fermentation liquid, and poor control of root-knot nematodes. In Comparative Examples 6 and 7, ceramic membranes were prepared using polyimide and polyethersulfone, respectively. The selectivity and separation effect of these membranes were not as good as the ceramic membranes prepared using a composite of polyimide and polyethersulfone in Examples 2-4, thus resulting in a worse control effect of root-knot nematodes compared to Examples 2-4.

[0073] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and embodiments shown and described herein.

Claims

1. An ecological control method for root-knot nematode disease, characterized in that, Includes the following steps: Crops are planted in soil mixed with earthworm castings, and during the crop growth process, they are regularly irrigated with earthworm fermentation liquid diluted with water. The preparation process of the earthworm fermentation liquid is as follows: Step 1: Take earthworms, grind and crush them into a slurry, and place it in a fermentation reactor for fermentation. During the fermentation process, add chitosan, humic acid, Bacillus coagulans and agar powder. The weight ratio of chitosan, humic acid, Bacillus coagulans and agar powder to earthworms is (0.3~0.5):(0.1~0.3):(0.1~0.3):(0.6~0.8):

1. The fermentation temperature is 45~50℃ and the fermentation time is 7~15 days, thereby obtaining a fermented mixture. Step 2: The fermentation mixture is initially filtered through gauze, and the filtrate is then finely filtered through a ceramic membrane tube assembly. The filtrate is collected to obtain the earthworm fermentation liquid. The ceramic membrane tube group in step two is composed of two sets of ceramic membrane tubes connected in series. Each set of ceramic membrane tubes is filled with 50 to 80 ceramic membrane cores. The average pore size of the ceramic membrane cores is 100 to 500 nm. The method for preparing the ceramic membrane core is as follows: Step a: Add N-methylpyrrolidone to the polyimide precursor polyamic acid, stir and mix evenly, add yttrium-stabilized zirconia ceramic powder with an average particle size of 200 nm, stir and mix evenly, add polyethersulfone again, stir and mix evenly, and degas to obtain casting solution, wherein the weight ratio of polyamic acid, N-methylpyrrolidone, yttrium-stabilized zirconia ceramic powder and polyethersulfone is (17~30):(40~60):(40~55):(17~30); Step b: Using a mixture of N-methylpyrrolidone and water at a weight ratio of 19:1 as the core liquid, the casting liquid is extruded through a spinneret under a nitrogen pressure of 0.1 MPa and immersed in a water tank for 6-12 hours. The resulting wet film is then removed and dried. Step c: Increase the temperature of the dried wet film to 500°C at a heating rate of 2°C / min and keep it at that temperature for 2 hours. Then increase the temperature to 800°C at a heating rate of 3°C / min and keep it at that temperature for 5 hours. Next, increase the temperature to 1500°C at a heating rate of 5°C / min and keep it at that temperature for 8 hours. Finally, gradually cool the film to room temperature at a cooling rate of 5°C / min to obtain the ceramic film core.

2. The ecological control method for root-knot nematode disease as described in claim 1, characterized in that, The amount of earthworm castings added is 100-200 kg / mu.

3. The ecological control method for root-knot nematode disease as described in claim 1, characterized in that, The irrigation volume of the earthworm fermentation liquid is 5~20 L / mu.

4. The ecological control method for root-knot nematode disease as described in claim 1, characterized in that, The thickness of the ceramic membrane core is 100~200μm.

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