A multi-shell thermophilic composite microbial agent, a preparation method and application thereof

Abstract

The application discloses a multi-shell thermophilic composite microbial agent, which is composed of a deodorization microbial group inner core, a nitrogen preservation microbial layer, a composite microbial layer for promoting decay and heat production, and a high-efficiency enzyme layer from inside to outside. The application also discloses a preparation method of the multi-shell thermophilic composite microbial agent and application of the multi-shell thermophilic composite microbial agent in wet garbage treatment. The multi-shell thermophilic composite microbial agent can keep nitrogen in the process of wet garbage super-high-temperature aerobic fermentation, thereby improving fertilizer efficiency; nitrogen loss in the form of NH3 and N2O is reduced, NH3 is reduced by more than 60% in the process of fermentation compared with the process of ordinary super-high-temperature aerobic fermentation, and odor generation is effectively reduced; the multi-shell thermophilic composite microbial agent promotes decay and heat production and is suitable for the degradation characteristics of wet garbage; therefore, the application can prepare organic slow-release fertilizer in a low-cost, quick and simple method, coal gangue is used to inhibit denitrification, soil fertility is effectively improved, and good ecological and economic benefits are achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of wet garbage aerobic fermentation, and particularly relates to a multi-shell thermophilic composite microbial agent and a preparation method and application thereof. BACKGROUND

[0002] With the rapid development of social economy and the deepening of garbage classification work in China, the quantity of wet garbage of urban residents has increased rapidly, and the wet garbage has high water content and high organic matter, which is easy to breed bacteria and cause corruption and odor. The traditional garbage physical treatment process is difficult to properly solve the above problems and further resource utilization. The biological aerobic fermentation treatment has obvious advantages in treating wet garbage due to its simple operation, low cost and high resource utilization efficiency.

[0003] The super-high-temperature aerobic fermentation is an improved technology of traditional aerobic fermentation, which can effectively improve the fermentation temperature and efficiently kill pathogens to realize the full composting and stabilization of organic matter. However, the technology will emit a large amount of harmful odor containing ammonia gas during operation, which pollutes the environment and reduces the quality of compost. Therefore, controlling the nitrogen loss in super-high-temperature aerobic fermentation is the key to improving the quality of compost. At present, some fermentation microbial agents and deodorization and nitrogen preservation microbial agents have been developed for traditional aerobic fermentation, but there is a lack of thermophilic composite microbial agents with super-high-temperature resistance (≥80℃), high nitrogen preservation efficiency and coupling preparation of organic slow-release fertilizer for application in super-high aerobic fermentation process. The odor and fertilizer efficiency problems seriously limit the popularization and application of biological fermentation treatment. SUMMARY

[0004] The present application aims to solve the problems of a large amount of odor produced by the thermophilic composite microbial agent in the super-high-temperature aerobic fermentation process and low fertilizer efficiency in the prior art, and provides a multi-shell thermophilic composite microbial agent, a preparation method and application thereof.

[0005] To solve the above technical problems, the technical solution adopted by the present application is as follows: a multi-shell thermophilic composite microbial agent, which is composed of a deodorization microbial group core, a nitrogen preservation microbial layer, a composting and heat production composite microbial layer and a high-efficiency enzyme layer from inside to outside.

[0006] The present application also provides a preparation method of the above multi-shell thermophilic composite microbial agent, which comprises the following steps:

[0007] S1, constructing a deodorization microbial group core: configuring a culture solution, adding 0.1-1 mg / L of an organic flocculant, Lactococcus lactis and Saccharomyces cerevisiae to the culture solution, and culturing at a temperature of 40-60℃ for 24-48 h to preliminarily form a deodorization microbial group core;

[0008] S2, constructing a nitrogen preservation microbial layer: continuing to add 5-30 mg·L -1 of FeCl3 solution and 0.5-2 mg·L -1 of (NH4)2MO4O13 The solution contains Bacillus polymyxa, Pseudomonas, and Bacillus licheniformis, and is cultured at a temperature of 50–65℃ for 12–36 hours to form a nitrogen-retaining bacterial layer.

[0009] S3. Construct a decomposition-promoting and heat-generating complex bacterial layer: Continue to add folic acid, thermophilic neobacterium, thermophilic lipophilic gespores, common thermophilic actinomycetes, cottony thermophilic mold, and firm brittle thermophilic ascomycetes to the culture medium. The culture temperature is 60-75℃ and the culture time is 24-36h to form a decomposition-promoting and heat-generating complex bacterial layer.

[0010] S4. Constructing a high-efficiency enzyme layer: Continue to add brewer's malt to the culture medium at a ratio of 10-20 g / L, and culture at a temperature of 60-75℃. Slightly aerate and shake for 6-12 hours to form a high-efficiency enzyme layer.

[0011] S5. Obtaining the bacterial agent: Add diatomaceous earth to the culture medium at a ratio of 2-4 g / L, stir evenly, and dry or freeze-dry to obtain the bacterial agent.

[0012] Preferably, the mass ratio of the thermophilic neobacterium, thermophilic lipophilic genomic bacillus, common thermophilic actinomycetes, cottony thermophilic mold, firm thermophilic ascomycetes, polymyxa, pseudomonads, licheniformis, lactococcus lactis, and saccharomyces cerevisiae is (1-1.5):(0.5-1.5):(1-2):(2-3):(1-4):(2-3):(1-3):(1.5-2):(2-4):(0.5-2).

[0013] Preferably, in step S1, the culture solution is formulated with 8-10 g / L of tryptone. -1 Sodium chloride 2-6 g·L -1 2-4 g / L of glucose -1 Dipotassium hydrogen phosphate 1.5–2 g·L -1 2-4 g / L of yeast extract -1 The pH value is 6.5 to 7.8.

[0014] Preferably, the organic flocculant is one or more of carboxymethyl cellulose, sodium polyacrylate, and polyamine polymers.

[0015] More preferably, the amount of folic acid added is 0.02-0.08% (m / v) of the bacterial culture medium.

[0016] This invention also provides the application of the above-mentioned multi-shell thermophilic compound microbial agent in wet waste treatment. The multi-shell thermophilic compound microbial agent is added to the wet waste pile and synergistically fermented with coal gangue at ultra-high temperature aerobic fermentation. After fermentation at a temperature exceeding 80°C for at least 7 days, and after aging for 10 to 20 days, it can be used as an organic slow-release fertilizer.

[0017] Preferably, the coal gangue is a silica-alumina type coal gangue, and the amount added is 1-5% of the mass of the wet waste pile.

[0018] Preferably, after the ultra-high temperature aerobic fermentation is started, the addition of microbial agents, adjustment of aeration rate and stirring frequency include: adding the multi-shell thermophilic composite microbial agent when the pile temperature is 30-45℃, with an addition amount of 0.1-0.5% of the pile mass; supplying oxygen through air pump, with an initial aeration rate of 0.4-0.5 L / (min·kg), and adjusting the aeration rate to 50-60% of the initial rate when the temperature is ≥75℃; not stirring when the pile temperature is below 60℃; and stirring at a frequency of 15-20 r / d when the temperature is ≥60℃.

[0019] The beneficial effects of this invention are as follows:

[0020] (I) The multi-shell thermophilic compound microbial agent in this invention is composed of four major microbial groups: thermogenic bacteria, putrefactive bacteria, nitrogen-retaining bacteria and deodorizing bacteria. Antagonism experiments have shown that the contained microbial species have no obvious antagonistic effect and can accumulate and grow. Among them, the thermogenic bacteria are metabolically active and can generate heat quickly; thermophilic neobacterium has strong salt and heat resistance; thermophilic lipophilic gespores can secrete enzymes such as thermophilic amylase and thermophilic protease; and common thermophilic actinomycetes can secrete esterases. In the putrefactive bacteria, the cottony thermophilic mold and the brittle thermophilic ascomycetes can produce xylanase, cellulase, amylase, and protease, which are used for the targeted putrefaction of lignocellulose and to accelerate the fermentation process. In the nitrogen-retaining bacteria, the polymyxa, pseudomonas, and licheniformis can supplement the nitrogen cycle of the indigenous bacteria in the ammoniation, nitrification, and denitrification processes, effectively reducing nitrogen loss. The deodorizing bacteria use common and easily cultured lactococci and brewer's yeast, which can rapidly proliferate, to effectively remove odors during the fermentation process and regulate the pH of the compost pile.

[0021] (II) The multi-shell thermophilic compound microbial agent of this invention constructs a multi-shell structure, consisting of an inner core of deodorizing bacteria, a nitrogen-retaining bacterial layer, a composting and heat-generating compound microbial layer, and a high-efficiency enzyme layer from the inside out. This effectively protects the composting enzyme system and the nitrogen-retaining enzyme system, and can enrich the microbial community. Therefore, the multi-shell thermophilic compound microbial agent of this invention can retain nitrogen during the ultra-high temperature aerobic fermentation of wet waste, thereby improving fertilizer efficiency; at the same time, it reduces the loss of nitrogen in the form of NH3, N2O, etc., and the NH3 is reduced by more than 60% compared with the ordinary ultra-high temperature aerobic fermentation process, effectively reducing odor generation; and it promotes composting and heat generation, adapting to the degradation characteristics of wet waste.

[0022] (III) This invention utilizes the porous structure of microbial flocs and diatomaceous earth to greatly improve the microbial adsorption rate, efficiently prepare thermophilic compound microbial agents, and promote full contact between microorganisms and materials during fermentation to quickly form dominant microbial communities.

[0023] (iv) This invention prepares organic slow-release fertilizer in a low-cost, fast and simple way, and uses coal gangue to inhibit denitrification, effectively improving soil fertility and having good ecological and economic benefits.

[0024] All strains used in this invention are publicly reported strains that can be obtained commercially by the public, specifically:

[0025] Lactococcus lactis with accession number CGMCC1.12794 was deposited on January 17, 2014, at the China General Microbiological Culture Collection Center (CGMCC), located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. It can be ordered from CGMCC and is deposited until 2044.

[0026] Saccharomyces cerevisiae with accession number CICC 30240, deposited on September 15, 1976, is deposited at the China Industrial Microbial Culture Collection Center (CICC), located at Building 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing. It can be ordered from CICC.

[0027] The accession number is CGMCC1.15984, and the deposit date is November 30, 2016. The depositary institution is the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. It can be ordered from the China General Microbiological Culture Collection Center (CGMCC), and the deposit period is until 2046.

[0028] The Pseudomonas sp. with accession number CICC 20581 was deposited on March 26, 2006, by the China Industrial Microbial Culture Collection Center (CICC), located at Building 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing. It can be ordered from CICC.

[0029] Bacillus licheniformis with accession number CICC 10291, deposited on January 1, 2004, by the China Industrial Microbial Culture Collection Center (CICC), located at Building 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing, can be ordered from CICC.

[0030] The thermophilic *Novibacillus thermophilus* with accession number CGMCC1.12771 was deposited on January 8, 2014, at the China General Microbiological Culture Collection Center (CGMCC), located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. It can be ordered from CGMCC and is deposited until 2044.

[0031] Geobacillus stearothermophilus, with accession number CGMCC1.3404, was deposited on June 1, 2003, at the China General Microbiological Culture Collection Center (CGMCC), located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. It can be ordered from CGMCC and is deposited until 2033.

[0032] Thermoactinomyces vulgaris, with accession number CICC 10672, was deposited on April 27, 2013, by the China Industrial Microbial Culture Collection Center (CICC), located at Building 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing. It can be ordered from CICC.

[0033] Thermomyces lanuginosus, with accession number CICC 2691, was deposited on January 29, 2015, by the China Industrial Microbial Culture Collection Center (CICC), located at Building 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing. It can be ordered from CICC.

[0034] The thermophilic ascomycete *Thermoascus crustaceus*, with accession number CICC 41718, was deposited on December 4, 2018, by the China Industrial Microbial Culture Collection Center (CICC), located at Building 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing. It can be ordered from CICC. Detailed Implementation

[0035] The present invention will be further described below with reference to specific embodiments.

[0036] In the following embodiments, the community wet waste used as the material to be processed has a moisture content of about 80%, and the auxiliary material is green waste, including weeds and leaves. The two are mixed to adjust the moisture content of the material to about 55%, and the mixed pile is broken into clumps with a diameter of less than 10cm and stirred evenly.

[0037] The bacterial culture medium formula is: 10 g / L tryptone. -1 3 g·L sodium chloride -1 2g / L glucose -1 Dipotassium hydrogen phosphate 1.5 g·L -1 3g·L yeast extract -1 The pH value is 6.8.

[0038] The coal gangue used is silicoaluminate type; the organic flocculant is sodium polyacrylate; the folic acid is a solid powder with a purity ≥97%; the diatomaceous earth has a density ≤0.40 and a specific surface area of ​​30–55 g / cm³. 2 The chemical composition is 65-68% SiO2, 15-17% Al2O3, 4-6% Fe2O3, 0.4% CaO, 0.5% MgO, 0.6% (TiO2+MnO2), and 2.0-2.2% (Na2O+K2O).

[0039] Example 1

[0040] 1. Preparation of thermophilic compound bacterial agent:

[0041] (1) Constructing the core of the deodorizing bacteria: Prepare a culture solution, add 0.1 mg / L sodium polyacrylate, Lactococcus lactis and Saccharomyces cerevisiae to the culture solution, culture at 40℃ for 24 h, and you will see flocs in the solution, which are the core of the deodorizing bacteria.

[0042] (2) Constructing a nitrogen-retaining bacterial layer: Continue to add 10 mg·L⁻¹ to the culture medium. -1 FeCl3 solution, 1 mg·L -1 (NH4)2MO4O 13 The solution contained *Paenibacillus polymyxa*, *Pseudomonas sp.*, and *Bacillus licheniformis*, and was cultured at 50°C for 12 hours to enhance nitrogenase activity and form a nitrogen-retaining bacterial layer.

[0043] (3) Construction of a decomposition-promoting and thermogenic composite bacterial layer: Continue to add 0.02% (m / v) of folic acid, *Novibacillus thermophilus*, *Geobacillus stearothermophilus*, *Thermoactinomyces vulgaris*, *Thermomyces lanuginosus*, and *Thermoascus crustaceus* to the culture medium. The culture temperature is 60℃, and the culture time is 24 hours to form a decomposition-promoting and thermogenic composite bacterial layer. The bacterial strain ratio is: *Novibacillus thermophilus* : *Geobacillus stearothermophilus* : *Thermoactinomyces vulgaris* : *Thermomyces lanuginosus* : *Thermoascus crustaceus* : *Paenibacillus* polymyxa: Pseudomonas sp.: Bacillus licheniformis: Lactococcus lactis: Saccharomyces cerevisiae = 1:0.5:1:2:1:2:1:1.5:2:0.5.

[0044] (4) Constructing a high-efficiency enzyme layer: Add beer lees to the culture medium at a ratio of 10 g / L, culture at 60°C, aerate slightly and shake for 6 h to stimulate the putrefactive bacteria to secrete large amounts of xylanase and cellulase, forming a high-efficiency enzyme layer.

[0045] (5) Obtaining bacterial agent: Add diatomaceous earth to the culture medium at a ratio of 2.5 g / L, stir evenly and dry to obtain bacterial agent.

[0046] 2. Start aerobic fermentation: Put 1t of the initially prepared material to be treated into the reactor for aerobic fermentation, add 10kg of coal gangue, control the initial aeration rate to 0.4L / (min·kg), and monitor the temperature of the pile.

[0047] 3. Initiate ultra-high temperature aerobic fermentation: When the pile temperature rises to 35℃, add 0.2% of the pile mass of thermophilic compound microbial agent and quickly mix it with the material, then do not stir; when the pile temperature rises to 60℃, the stirring frequency is 15 r / d; when the pile temperature rises to 75℃, adjust the aeration rate to 0.2 L / (min·kg). Add 500 kg of the material to be treated daily, and after the first 20 days, all the fermented material should be back-mixed.

[0048] 4. Preparation of organic slow-release fertilizer: Starting from day 21, take out 50kg of fermented material every day and let it stand for 15 days to age.

[0049] 5. The NH3 emissions after the ultra-high temperature aerobic fermentation temperature of wet waste was greater than 60℃, as well as the total nitrogen, humus content, pH, and seed germination index in the organic slow-release fertilizer were measured. The results are shown in Table 1.

[0050] Example 2

[0051] The preparation method in this embodiment is the same as in Example 1, except that the thermophilic compound bacterial agent is prepared as follows:

[0052] 1. Adjust the bacterial culture ratio to: Novibacillus thermophilus: Geobacillus stearothermophilus: Thermoactinomyces vulgaris: Thermomyces lanuginosus: Thermoascus crustaceus: Paenibacillus polymyxa: Pseudomonas sp.: Bacillus licheniformis: Lactococcus lactis: Saccharomyces cerevisiae = 1.5:1.5:2:3:4:3:3:2:4:2.

[0053] 2. The amount of folic acid added when constructing the putrefactive and thermogenic bacterial layer was 0.05% (m / v).

[0054] The NH3 emissions, total nitrogen, humus content, pH, and seed germination index of the organic slow-release fertilizer were measured after the ultra-high temperature aerobic fermentation temperature of wet waste was greater than 60℃. The results are shown in Table 1.

[0055] Example 3

[0056] The preparation method in this embodiment is the same as in Example 1, except that the thermophilic compound bacterial agent is prepared as follows:

[0057] 1. Adjust the bacterial strain ratio to: Novibacillus thermophilus: Geobacillus stearothermophilus: Thermoactinomyces vulgaris: Thermomyces lanuginosus: Thermoascus crustaceus: Paenibacillus polymyxa: Pseudomonas sp.: Bacillus licheniformis: Lactococcus lactis: Saccharomyces cerevisiae = 1.2:1:1.5:2.5:2.5:2.5:1.8:1.7:3:1.2.

[0058] 2. The amount of folic acid added when constructing the putrefactive and thermogenic bacterial layer was 0.04% (m / v).

[0059] The NH3 emissions, total nitrogen, humus content, pH, and seed germination index of the organic slow-release fertilizer were measured after the ultra-high temperature aerobic fermentation temperature of wet waste was greater than 60℃. The results are shown in Table 1.

[0060] Table 1. Relevant operating parameters, NH3 emissions, and organic slow-release fertilizer indicators for Examples 1-3

[0061]

[0062]

[0063] Comparative Example 1

[0064] The preparation method of this comparative example is the same as that of Example 1, except that steps (1) and (2) of preparing the thermophilic compound bacterial agent are adjusted as follows:

[0065] (1) Construction of nitrogen-retaining bacterial core: Prepare culture solution, add 0.1 mg / L sodium polyacrylate and 10 mg·L⁻¹ sodium acrylate to the culture solution. -1 FeCl3 solution, 1 mg·L -1 (NH4)2MO4O 13 The solution contained *Paenibacillus polymyxa*, *Pseudomonas* sp., and *Bacillus licheniformis*, cultured at 50℃ for 12 hours to enhance nitrogenase activity and form a nitrogen-retaining microbial core.

[0066] (2) Constructing the deodorizing bacteria layer: Continue to culture Lactococcus lactis and Saccharomyces cerevisiae in the culture medium at a temperature of 40℃ for 24 hours. Flocs will be produced in the solution, which is the deodorizing bacteria layer.

[0067] The NH3 emissions, total nitrogen, humus content, pH, and seed germination index in organic slow-release fertilizer were measured after the ultra-high temperature aerobic fermentation temperature of wet waste was greater than 60℃. The results are shown in Table 2.

[0068] Comparative Example 2

[0069] The preparation method of this comparative example is the same as that of Example 1. The difference is that step (2) "constructing nitrogen-retaining bacterial layer" is omitted when preparing thermophilic compound bacterial agent. After forming the deodorizing bacterial core, the decomposition-promoting and heat-generating compound bacterial layer is directly constructed.

[0070] The NH3 emissions, total nitrogen, humus content, pH, and seed germination index in organic slow-release fertilizer were measured after the ultra-high temperature aerobic fermentation temperature of wet waste was greater than 60℃. The results are shown in Table 2.

[0071] Comparative Example 3

[0072] The preparation method of this comparative example is the same as that of Example 1. The difference is that when preparing the thermophilic compound bacterial agent, step (3) "constructing the compost-promoting and heat-generating compound bacterial layer" is omitted, and after constructing the nitrogen-retaining bacterial layer, the high-efficiency enzyme layer is directly attempted to be constructed.

[0073] Since the fermentation temperature did not reach above 60℃, the NH3 emission, total nitrogen, humus content, pH, and seed germination index in the organic slow-release fertilizer were measured after the ultra-high temperature aerobic fermentation temperature of wet waste exceeded 55℃. The results are shown in Table 2.

[0074] Comparative Example 4

[0075] The preparation method of this comparative example is the same as that of Example 1. The difference is that no organic flocculant is added in step (1) when preparing the thermophilic compound bacterial agent. As a result, all the bacterial groups are in a dispersed state and cannot form a multi-shell structure.

[0076] The NH3 emissions, total nitrogen, humus content, pH, and seed germination index in organic slow-release fertilizer were measured after the ultra-high temperature aerobic fermentation temperature of wet waste was greater than 60℃. The results are shown in Table 2.

[0077] Comparative Example 5

[0078] The preparation method for this comparative example is the same as that for Example 1, except that 10 kg of coal gangue is not added when fermentation is started. The NH3 emissions, total nitrogen, humus content, pH, and seed germination index in the organic slow-release fertilizer were measured after the ultra-high temperature aerobic fermentation temperature of wet waste exceeded 60℃. The results are shown in Table 2.

[0079] Table 2 shows the NH3 emissions and related indicators of organic slow-release fertilizers in Comparative Examples 1–5.

[0080]

[0081] In Comparative Example 1, the nitrogen-retaining bacteria form the core, and the nitrogen in the pile is preemptively utilized and decomposed by the deodorizing bacteria. Therefore, the effects of nitrogenase, ammonifying enzyme, etc., are not fully utilized, resulting in poor nitrogen retention. In Comparative Example 2, the agent lacks nitrogen-retaining bacteria, and a large amount of nitrogen in the pile is converted into ammonia and dissipates, resulting in poor nitrogen retention. In Comparative Example 3, the agent lacks a heat-generating and decay-promoting bacterial layer, resulting in slow temperature rise in the pile, difficulty in degrading lignocellulose, and hindered humification, resulting in low humus content and strong odor. In Comparative Example 4, the absence of a shell structure and low bacterial enrichment makes it difficult to quickly form a dominant bacterial group, leading to slow onset and insignificant nitrogen retention. In Comparative Example 5, the lack of co-fermentation of coal gangue and materials weakens the inhibitory effect on denitrification, causing some nitrogen loss.

[0082] The results in Tables 1 and 2 show that the multi-shell thermophilic compound microbial agent prepared in this invention has significant nitrogen retention, deodorization, and decomposition-promoting effects. It has significant advantages in reducing odor, increasing fermentation temperature, and promoting humification. Disrupting the shell sequence or using conventional dispersed culture will reduce its effects. In addition, the method of preparing organic slow-release fertilizer using the microbial agent provided in this invention can fully retain fertilizer efficacy and improve seed germination rate, thereby meeting the requirements of the "Organic Fertilizer" standard NY / T 525-2021.

[0083] This specification is intended to be illustrative rather than restrictive. Based on this invention, those skilled in the art can make substitutions and modifications to some of the technical features without creative effort, and all such modifications are within the scope of protection of this invention.

Claims

1. A multi-shell thermophilic compound bacterial agent, characterized in that, The multi-shell thermophilic compound bacterial agent consists of, from the inside out, a deodorizing bacterial core, a nitrogen-retaining bacterial layer, a putrefactive and thermogenic compound bacterial layer, and a highly efficient enzyme layer; the preparation method of the multi-shell thermophilic compound bacterial agent includes the following steps: S1. Constructing the core of the deodorizing bacteria community: Prepare a culture solution, add 0.1~1 mg / L of organic flocculant, lactococcus lactis and brewer's yeast to the culture solution, culture at 40~60℃ for 24~48h, and initially form the core of the deodorizing bacteria community; S2. Constructing a nitrogen-retaining bacterial layer: Continue adding 5~30 mg·L⁻¹ to the culture medium. -1 FeCl3 solution, 0.5~2 mg·L -1 (NH4)2MO4O 13 The solution contains Bacillus polymyxa, Pseudomonas, and Bacillus licheniformis. The culture temperature is 50-65℃ and the culture time is 12-36h to form a nitrogen-retaining bacterial layer. S3. Construct a decomposition-promoting and heat-generating complex bacterial layer: Continue to add folic acid, thermophilic neobacterium, thermophilic lipophilic gesperm, common thermophilic actinomycetes, cottony thermophilic mold, and firm brittle thermophilic ascomycetes to the culture medium. The culture temperature is 60~75℃ and the culture time is 24~36h to form a decomposition-promoting and heat-generating complex bacterial layer. S4. Constructing a high-efficiency enzyme layer: Continue to add brewer's malt to the culture medium at a ratio of 10~20g / L, and culture at a temperature of 60~75℃. Slightly aerate and shake for 6~12h to form a high-efficiency enzyme layer. S5. Obtaining bacterial agent: Add diatomaceous earth to the culture medium at a ratio of 2~4g / L, stir evenly, and dry or freeze dry to obtain bacterial agent; The mass ratio of the following bacteria is (1-1.5):(0.5-1.5):(1-2):(2-3):(1-4):(2-3):(1-3):(1.5-2):(2-4):(0.5-2).

2. The multi-shell thermophilic compound bacterial agent according to claim 1, characterized in that, In step S1, the culture solution is prepared using the following formula: 8-10 g / L tryptone. -1 Sodium chloride 2~6 g·L -1 2-4 g / L of glucose -1 Dipotassium hydrogen phosphate 1.5~2 g·L -1 Yeast extract 2-4 g / L -1 The pH value is 6.5~7.

8.

3. The multi-shell thermophilic compound bacterial agent according to claim 2, characterized in that, The organic flocculant is one or more of carboxymethyl cellulose, sodium polyacrylate, and polyamine polymers.

4. The multi-shell thermophilic compound bacterial agent according to claim 2, characterized in that, The folic acid addition amount is 0.02~0.08% (m / v) of the bacterial culture medium.

5. The application of the multi-shell thermophilic compound microbial agent as described in any one of claims 1 to 4 in the treatment of wet waste, characterized in that, Adding multi-shell thermophilic compound microbial agent to wet waste piles and co-fermenting it with coal gangue at ultra-high temperature aerobic fermentation, fermenting at a temperature exceeding 80℃ for at least 7 days, and aging for 10-20 days, allows it to be used as organic slow-release fertilizer.

6. The application according to claim 5, characterized in that, The coal gangue is a silica-alumina type, and the amount added is 1-5% of the mass of the wet waste pile.

7. The application according to claim 5, characterized in that, After the ultra-high temperature aerobic fermentation is started, the following steps are taken: adding microbial agents, adjusting the aeration rate and stirring frequency, including: adding the multi-shell thermophilic composite microbial agent when the pile temperature is 30~45℃, with an addition amount of 0.1~0.5% of the pile mass; supplying oxygen through air pumps, with an initial aeration rate of 0.4~0.5L / (min·kg), and adjusting the aeration rate to 50~60% of the initial rate when the temperature is ≥75℃; not stirring when the pile temperature is below 60℃; and stirring at a frequency of 15~20 r / d when the temperature is ≥60℃.

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