A magnetic agarose microsphere production device

Abstract

The application discloses a kind of magnetic agarose microsphere production devices, including aqueous phase preparation component, atomization cooling component, circulating refrigeration component and control component, atomization cooling component is installed in the top of circulating refrigeration component, aqueous phase preparation component is installed in the top of atomization cooling component, control component is electrically connected with aqueous phase preparation component and circulating refrigeration component;Atomization cooling component includes nested inner atomization box and outer atomization box and flow cell, and the bottom of outer atomization box is connected with flow cell, the top of inner atomization box is connected with aqueous phase preparation component, aqueous phase preparation component is connected with external air pressure generating device, and inner atomization box is driven to slide relative to outer atomization box along the direction perpendicular to horizontal plane, and flow cell is connected with circulating refrigeration component.The magnetic agarose microsphere production device process flow is shorter, operation is more convenient, and the scale, semi-continuous production of magnetic agarose microsphere can be realized.

Description

Technical Field

[0001] This invention relates to the field of biomaterial preparation technology, and more specifically to a magnetic agarose microsphere production apparatus. Background Technology

[0002] Magnetic agarose microspheres are composite microspheres composed of magnetic particles (commonly Fe3O4) and agarose microspheres. Agarose is a natural linear polysaccharide; the gel it forms is highly porous and hydrophilic. It contains no charged groups, thus avoiding non-specific adsorption behavior, and its abundant hydroxyl groups on the molecular chain are easily activated for functionalization. Therefore, agarose microspheres have become the most widely used chromatography medium in bioseparation processes. Magnetic agarose microspheres combine the excellent properties of magnetic separation technology with those of agarose gels. By applying an external magnetic field, they can be rapidly separated from liquid samples, making them an important carrier tool in the fields of medicine and molecular biology. Their applications in enzyme and cell immobilization, and the separation of nucleic acids, proteins, and cells are increasingly widespread.

[0003] Existing methods for preparing magnetic agarose microspheres mainly include in-situ composite methods and droplet solidification methods. The in-situ composite method can prepare agarose-coated magnetic Fe3O4 nanospheres with an average particle size of 20 nm, but it is rarely used in the biomedical field. Microspheres prepared by the droplet solidification method have a dispersed structure, meaning that Fe3O4 particles are uniformly dispersed within agarose gel beads, and the microsphere particle size is typically tens to hundreds of micrometers. There are two main methods for preparing agarose droplets: emulsification and spraying. The corresponding microsphere preparation methods are "emulsification-cooling" and "spray-cooling," respectively.

[0004] Among them, the traditional "emulsification-cooling method" produces microspheres with a wide particle size distribution (usually multi-peaked) and poor batch-to-batch repeatability. Furthermore, due to limitations in emulsification principles and equipment costs, continuous production is difficult to achieve. Microfluidic droplet methods and membrane emulsification methods involve excessively small fluid fluxes, resulting in extremely slow preparation processes that are difficult to scale up for mass production. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide a magnetic agarose microsphere production device that achieves the production of magnetic agarose microspheres through an "atomization-cooling method" to shorten the process flow, simplify operation, and improve the production efficiency of magnetic agarose microspheres.

[0006] To achieve the above objectives, the present invention provides a magnetic agarose microsphere production apparatus, comprising an aqueous phase preparation component, an atomization and cooling component, a circulating refrigeration component, and a control component. The atomization and cooling component is installed on top of the circulating refrigeration component, and the aqueous phase preparation component is also installed on top of the atomization and cooling component. The control component is electrically connected to both the aqueous phase preparation component and the circulating refrigeration component. The atomization and cooling component includes a nested inner atomization box and an outer atomization box, as well as a flow pool. The bottom of the outer atomization box is connected to the flow pool. The aqueous phase preparation component is connected to the top of the inner atomization box and is connected to an external air pressure generating device. The inner atomization box is driven to slide relative to the outer atomization box in a direction perpendicular to the horizontal plane. The flow pool is connected to the circulating refrigeration component.

[0007] Preferably, the aqueous phase preparation assembly includes an insulated box, a feeding and stirring assembly, a liquid tank, and a nozzle. The feeding and stirring assembly is detachably installed on the top of the insulated box. The liquid tank and the nozzle are installed inside the insulated box. The feeding and stirring assembly is connected to the liquid tank. The nozzle is connected to the outlet of the liquid tank through a pipe. The nozzle tip passes through the bottom surface of the insulated box and the top surface of the inner atomizing box, extending into the interior of the inner atomizing box.

[0008] Preferably, the nozzle is a dual-fluid nozzle, the bottom of the nozzle is a nozzle head, and the side of the nozzle has three interfaces, two of which are connected to an external air pressure generating device through pipes, and one of the interfaces is connected to the outlet of the liquid tank through a pipe.

[0009] Preferably, the nozzle is mounted at the bottom of the insulation box via a brass heating block.

[0010] Preferably, the feeding and stirring assembly includes a stirring device, a feeding trough, a sealing cover, and a first drive rod. The stirring device and the feeding trough are installed together with the sealing cover. The first drive rod is connected to the sealing cover and is also connected to the control assembly. The first drive rod drives the sealing cover to move in a direction perpendicular to the horizontal plane. The sealing cover is connected to the liquid tank. When the sealing cover is connected to the liquid tank, the stirring device extends into the liquid tank, and the feeding trough communicates with the liquid tank.

[0011] Preferably, the liquid tank has a tank body, and electric heating elements are mounted around the side of the tank body.

[0012] Preferably, the stirring device includes a motor, a reducer, a support, a stirring shaft, and several stirring paddles. The support is installed in conjunction with the sealing cover, the reducer is installed in conjunction with the support, the motor is driven by the reducer, the stirring shaft is driven by the reducer, and several stirring paddles are installed on the stirring shaft, with the stirring paddles extending into the liquid tank.

[0013] Preferably, a second drive rod is connected to the side of the insulation box, and the second drive rod is connected to the control component. The second drive rod drives the insulation box to move in a direction perpendicular to the horizontal plane, and the inner atomizing box moves synchronously during the movement of the insulation box.

[0014] Preferably, the top of the flow pool is connected to the outer atomizing box, the flow pool has an inclined surface inside, and an outlet and an inlet are respectively opened on the side of the flow pool. The outlet and the inlet are respectively connected to the circulating cooling component through pipes. The inlet is located on the higher side of the inclined surface of the flow pool, and the outlet is located on the lower side of the inclined surface of the flow pool. A settling tank is provided at the lowest end of the inclined surface of the flow pool, and a discharge port is opened at the bottom of the settling tank. The bottom of the discharge port is connected to a receiving tank.

[0015] Preferably, the circulating refrigeration assembly includes a support frame, with the flow pool mounted on top of the support frame. The receiving tank, heat exchanger, and diaphragm pump are disposed inside the support frame. The inlet of the heat exchanger is connected to the outlet of the diaphragm pump via a pipe, the inlet of the diaphragm pump is connected to the water outlet via a pipe, and the outlet of the heat exchanger is connected to the water inlet via a pipe.

[0016] Compared with existing technologies, the magnetic agarose microsphere production device disclosed in this invention has the following advantages: the magnetic agarose microsphere production device integrates the processing technology of agarose from "heating and dissolving" to "nozzle atomization" and then to "cooling and solidifying", resulting in a shorter process flow and more convenient operation. It can realize the large-scale and semi-continuous production of magnetic agarose microspheres, effectively improving the production efficiency of magnetic agarose microspheres; the magnetic agarose microsphere production device is also easy and quick to clean. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] like Figure 1The diagram shown is a structural schematic of a magnetic agarose microsphere production device according to the present invention.

[0019] like Figure 2 The image shown is a partial cross-sectional view of a magnetic agarose microsphere production apparatus according to the present invention.

[0020] like Figure 3 The diagram shown is a schematic diagram of the nozzle structure of a magnetic agarose microsphere production device according to the present invention.

[0021] like Figure 4 The diagram shown is a structural schematic of the stirring device in a magnetic agarose microsphere production apparatus according to the present invention.

[0022] like Figure 5 The image shown is a top view of the aqueous phase preparation component of a magnetic agarose microsphere production apparatus according to the present invention.

[0023] like Figure 6 The diagram shown is a schematic representation of the material tank in a magnetic agarose microsphere production device according to the present invention.

[0024] like Figure 7 The diagram shown is a schematic representation of the structure of the insulation box in a magnetic agarose microsphere production device according to the present invention.

[0025] like Figure 8 The diagram shown is a schematic representation of the atomizing box in a magnetic agarose microsphere production device according to the present invention.

[0026] like Figure 9 The diagram shown is a schematic diagram of the flow cell structure of a magnetic agarose microsphere production device according to the present invention.

[0027] like Figure 10 The diagram shown is a schematic representation of the circulating refrigeration component of a magnetic agarose microsphere production device according to the present invention.

[0028] like Figure 11 The diagram shown is a schematic representation of the control components of a magnetic agarose microsphere production device according to the present invention. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] like Figure 1 and Figure 2As shown, the magnetic agarose microsphere production apparatus of the present invention includes an aqueous phase preparation component I, an atomization and cooling component II, a circulating refrigeration component III, and a control component IV. The atomization and cooling component II is installed on top of the circulating refrigeration component III, and the aqueous phase preparation component II is also installed on top of the atomization and cooling component II. The control component IV is installed in conjunction with the circulating refrigeration component III and is electrically connected to the aqueous phase preparation component I and the circulating refrigeration component III. The atomization and cooling component II includes a nested inner atomization box 5 and an outer atomization box 7, and a flow pool 6. The bottom of the outer atomization box 7 is connected to the flow pool 6. The aqueous phase preparation component II is connected to the top of the inner atomization box 5 and is connected to an external air pressure generating device. The inner atomization box 5 is driven to slide relative to the outer atomization box 7 in a direction perpendicular to the horizontal plane. The flow pool 6 is located on top of the circulating refrigeration component III and is connected to the circulating refrigeration component III.

[0031] In use, raw materials are added through the aqueous phase preparation component I, which stirs and heats the raw materials. Then, the organic phase is added to the flow cell 6, and the organic phase is mixed and cooled by the circulating cooling component III, thus completing the preparation of the aqueous phase and the receiving liquid. Afterward, the inner atomizing box 5 is driven to move relative to the outer atomizing box 7, changing the height of the aqueous phase preparation component II relative to the flow cell 6. The aqueous phase preparation component II is driven to spray by an external air pressure generating device, creating a conical atomization zone in the inner atomizing box 5. The droplets in the atomization zone fall into the flowing oil phase in the flow cell 6 due to gravity, and are rapidly cooled and solidified into magnetic agarose microspheres. The solidified microspheres flow into the receiving tank 13, and after static stratification, the microspheres are washed with ethanol solutions of gradient concentrations and pure water, respectively, to complete the preparation.

[0032] Specifically, the aqueous phase preparation component I includes an insulated box 4, a feeding and stirring component, a liquid tank 10, and a nozzle 15. The feeding and stirring component is detachably installed on the top of the insulated box 4. The liquid tank 10 and the nozzle 15 are installed inside the insulated box 4. The feeding and stirring component is connected to the liquid tank 10. The nozzle 15 is connected to the outlet of the liquid tank 10 through a pipe, and the nozzle head of the nozzle 15 passes through the bottom surface of the insulated box 4 and the top surface of the inner atomizing box 5, extending into the interior of the inner atomizing box 5. See also Figure 3 Nozzle 15 is a dual-fluid nozzle. The bottom of nozzle 15 is the spray head. The side of nozzle 15 has three ports (S, Z, M). Two ports (S and Z) are connected to an external air pressure generating device through pipes, and one port (M) is connected to the discharge port of liquid tank 10 through a pipe. The air pressures involved in ports S, Z, and M are denoted as P. S P Z and P M See also Figure 7Preferably, the nozzle 15 is installed at the bottom of the insulation box 4 via a brass heating block 30, which can fix and heat the nozzle 15.

[0033] See Figures 4 to 7 The feeding and mixing assembly includes a mixing device 1, a feeding trough 2, a sealing cover 3, and a first drive rod 11. The mixing device 1 and the feeding trough 2 are installed together with the sealing cover 3. The first drive rod 11 is connected to the sealing cover 3 and is also connected to the control assembly for transmission. The first drive rod 11 drives the sealing cover 3 to move in a direction perpendicular to the horizontal plane. The sealing cover 3 is connected to the liquid tank 10. When the sealing cover 3 is connected to the liquid tank 10, the mixing device 1 extends into the liquid tank 10, and the feeding trough 2 communicates with the liquid tank 2.

[0034] The sealing cap 3 has an air inlet 23 and four sealing cap slots 21. The air inlet 23 is connected to an external air pressure generating device through a pipe. The top of the liquid tank 10 has four liquid tank slots 28 that respectively mate with the four sealing cap slots 21. When the sealing cap 3 mates with the liquid tank 10, screws 22 are inserted into the corresponding sealing cap slots 21 and liquid tank slots 28 to achieve a tight connection and seal between the sealing cap 3 and the liquid tank 10.

[0035] The liquid tank 10 has a tank body 25, and electric heating elements 26 are installed around the side of the tank body 25. The electric heating elements 26 can heat the raw material inside the tank body 25. The bottom of the tank body 25 has a discharge port 27, which is connected to the interface (M) on the side of the nozzle 15 through a pipe.

[0036] Two air ducts 24 are provided on the top of the insulated box 4. The pipes connected to the two interfaces (S, Z) of the nozzle 15 pass through the air ducts 24 into the interior of the insulated box 4. Several heating tubes 29 are installed inside the insulated box 4, preferably three heating tubes 29. The heating tubes 29 can heat the air inside the insulated box 4, so as to maintain a high temperature inside the insulated box 4, ensure that the agarose solution is in a dissolved state, and prevent the agarose solution from solidifying and causing blockage of the pipes when flowing in the pipes.

[0037] The stirring device 1 includes a motor 16, a reducer 17, a support 18, a stirring shaft 19, and several stirring paddles 20. The support 18 is installed in conjunction with the sealing cover 3, the reducer 17 is installed in conjunction with the support 18, the motor 16 is driven by the reducer 17, and the stirring shaft 19 is driven by the reducer 17. Several stirring paddles 20 are installed on the stirring shaft 19 and extend into the liquid tank 10. The motor 16 drives the stirring shaft 19 and the stirring paddles 20 to rotate, realizing the stirring and dispersion function of the liquid phase. Preferably, three stirring paddles 20 are installed on the stirring shaft 19. The installation position of the stirring paddles 20 on the stirring shaft 19 is adjustable, so that the installation position of the stirring paddles 20 can be adjusted according to the liquid level in the liquid tank 10 to achieve efficient stirring.

[0038] The side of the heat preservation box 4 is connected to the second drive rod 9, which is connected to the control component. The second drive rod 9 drives the heat preservation box 4 to move in a direction perpendicular to the horizontal plane. During the movement of the heat preservation box 4, the inner atomizing box 5 moves synchronously, changing the height of the inner atomizing box 5 relative to the flow pool 6.

[0039] See Figure 8 The inner atomizing box 5 has a nozzle locking hole 31 at its top, through which the nozzle 15 passes. Both the inner atomizing box 5 and the outer atomizing box 7 have observation windows 32 on their sides for easy observation of the atomization process. Preferably, an LED light 33 is installed on the exterior of the outer atomizing box 7 to further reduce the difficulty for the operator in observing the atomization process.

[0040] See Figure 9 and Figure 10 The top of the flow cell 6 is connected to the outer atomizing box 7. The flow cell 6 has an inclined surface 34. An outlet 37 and an inlet 38 are respectively opened on the side of the flow cell. The outlet 37 and inlet 38 are connected to the circulating cooling component via pipes. The inlet 38 is located on the higher side of the inclined surface 34, and the outlet 37 is located on the lower side. A settling tank 35 is provided at the lowest end of the inclined surface 34. A discharge port 36 is opened at the bottom of the settling tank 35, and the bottom of the discharge port 36 is connected to the receiving tank 13. A valve is installed on the discharge port 36. Droplets in the atomizing zone fall into the flowing oil phase in the flow cell 6 due to gravity, rapidly cool and solidify into magnetic agarose microspheres, and the solidified microspheres flow into the receiving tank 13.

[0041] The circulating refrigeration assembly includes a support frame, with a flow tank 6 mounted on top of the frame. Inside the frame are a receiving tank 13, a heat exchanger 12, and a diaphragm pump 14. The inlet 12 of the heat exchanger is connected to the outlet of the diaphragm pump 14 via a pipe, the inlet of the diaphragm pump 14 is connected to the outlet 37 via a pipe, and the outlet of the heat exchanger 12 is connected to the inlet 38 via a pipe. The heat exchanger 12 and the diaphragm pump 14 achieve circulation and refrigeration of the organic phase in the flow tank 6.

[0042] See Figure 11 Control component IV includes a control housing, a drive assembly installed inside the housing, and a control panel installed on the side of the housing. The control panel is equipped with an emergency stop switch 39, a main switch 40, a speed control switch 41, a heating element temperature control switch 42, a brass heating block temperature control switch 43, a liquid tank temperature control switch 44, a second drive lever switch 45, a first drive lever switch 46, an air circuit S switch 47, and a P switch. S Pressure regulating valve 48, air circuit M switch 49, P M Pressure regulating valve 50, air circuit Z switch 51 and P Z Pressure regulating valve 52. The operator can control the production process of the magnetic agarose microsphere production device through the switches and regulating valves on the control panel.

[0043] The production process of the magnetic agarose microsphere production device is as follows:

[0044] First, the aqueous phase raw materials (60g agarose, 45g magnetic core, 2.19g sodium chloride, and 1.5L water) are mixed, ultrasonically treated, and then added to the liquid tank 10 through the feeding trough 2. The first drive rod switch 46 is turned on, so that the sealing cover 3 fits into the liquid tank 10. The screw 22 is inserted into the sealing cover slot 21 and the liquid tank slot 28, and the screw 22 is tightened to seal the sealing cover 3 and the liquid tank 10. Then, the main switch 40, the speed control switch 41, the heating element temperature control switch 42, the brass heating block temperature control switch 43, and the liquid tank temperature control switch 44 are turned on in sequence. The stirring speed is set to 280 rpm, the heating temperature of the heating element 29 is set to 80℃, the heating temperature of the brass heating block 30 is set to 80℃, and the heating temperature of the liquid tank is set to 95℃. After the temperature in the liquid tank 10 reaches the set temperature, the timer is started, and the aqueous phase is heated, stirred, and dissolved for 1 hour.

[0045] The organic phase (15.8 kg cyclohexane and 632 g Span 85) was added to the flow cell 6. The diaphragm pump 14 and the external refrigeration device were turned on. The refrigeration temperature of the refrigeration device was set to 6°C. The organic phase was mixed and refrigerated (after 1 hour of refrigeration, the temperature of the receiving phase was basically stable at 7-8°C).

[0046] Once the aqueous phase and receiving liquid are ready, turn on the second drive rod switch 45. Adjust the height of the nozzle 15 from the flow tank 6 by adjusting the movement of the second drive rod 9. Then turn on the external air pressure generating device switch, and then turn on the air path Z switch 51, air path M switch 49 and air path S switch 47 in sequence. Rotate P Z Pressure regulating valve 52, P M Pressure regulating valves 50 and P SPressure regulating valve 48 is used to set the air pressure required for spraying. Subsequently, the aqueous phase is atomized by nozzle 15, producing a conical atomization zone in the inner atomization box 5. With the help of LED light 33, the atomization can be observed through observation window 32. The droplets in the atomization zone fall into the flowing oil phase in the flow tank 6 due to gravity, and quickly cool and solidify into magnetic agarose microspheres. The solidified microspheres flow along the inclined surface 34 of the flow tank into the settling tank 35. After spraying is completed, the microspheres and oil phase are discharged together from the discharge port and flow into the receiving tank 13 below. After settling and stratification, the microspheres are washed with ethanol solution of gradient concentration and pure water respectively.

[0047] The cleaning process of the magnetic agarose microsphere production device is as follows:

[0048] After the organic phase (receiving liquid) in the flow cell 6 is discharged together with the prepared microspheres, close the valve of the discharge port 36. Then, sequentially close the gas path M switch 49, gas path S switch 47, and gas path Z switch 51, and close the speed control switch 41 to stop stirring. Next, add sufficient hot water to the feed tank 10 through the feeding tank 2, turn on the speed control switch 41, and start stirring to dissolve the residual aqueous agarose solution on the wall of the feed tank 10 and the stirring shaft 19 in the hot water. After the feed tank 10 is cleaned, sequentially turn on the gas path M switch 49 and gas path S switch 47, and adjust P... M Pressure regulating valves 50 and P S The pressure regulating valve 48 allows the cleaning fluid to be sprayed from the nozzle 15. Repeat the above operation several times until the liquid tank 10, the water phase flow pipeline, and the nozzle 15 are completely cleaned. Detergent can be added to the flow tank 6 for further cleaning, and the cleaning fluid is discharged through the discharge port 36.

[0049] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A magnetic agarose microsphere production apparatus, characterized in that, The device includes a water phase preparation component, an atomization cooling component, a circulating cooling component, and a control component. The atomization cooling component is mounted on top of the circulating cooling component, and the water phase preparation component is also mounted on top of the atomization cooling component. The control component is electrically connected to both the water phase preparation component and the circulating cooling component. The atomization cooling component includes a nested inner atomization box and an outer atomization box, as well as a flow pool. The bottom of the outer atomization box is connected to the flow pool. The water phase preparation component is connected to the top of the inner atomization box and to an external air pressure generating device. The inner atomization box is driven to slide relative to the outer atomization box in a direction perpendicular to the horizontal plane. The flow pool is connected to the circulating cooling component. The aqueous phase preparation assembly includes an insulated box, a feeding and stirring assembly, a liquid tank, and a nozzle. The feeding and stirring assembly is detachably installed on the top of the insulated box. The liquid tank and the nozzle are installed inside the insulated box. The feeding and stirring assembly is connected to the liquid tank. The nozzle is connected to the outlet of the liquid tank through a pipe. The nozzle head passes through the bottom surface of the insulated box and the top surface of the inner atomizing box, extending into the interior of the inner atomizing box. The top of the inner atomizing box has a nozzle locking hole, and the nozzle head passes through the nozzle locking hole to pass through the top of the inner atomizing box. The side of the insulation box is connected to a second drive rod, which is connected to the control component. The second drive rod drives the insulation box to move in a direction perpendicular to the horizontal plane, and the inner atomizing box moves synchronously during the movement of the insulation box.

2. The magnetic agarose microsphere production apparatus as described in claim 1, characterized in that, The nozzle is a dual-fluid nozzle, with the nozzle head at the bottom and three ports on the side. Two of the ports are connected to an external air pressure generating device via pipes, and one port is connected to the outlet of the liquid tank via a pipe.

3. The magnetic agarose microsphere production apparatus as described in claim 1, characterized in that, The nozzle is mounted at the bottom of the insulated box via a brass heating block.

4. The magnetic agarose microsphere production apparatus as described in claim 1, characterized in that, The feeding and stirring assembly includes a stirring device, a feeding trough, a sealing cover, and a first drive rod. The stirring device and the feeding trough are installed together with the sealing cover. The first drive rod is connected to the sealing cover and is also connected to the control assembly. The first drive rod drives the sealing cover to move in a direction perpendicular to the horizontal plane. The sealing cover is connected to the liquid tank. When the sealing cover is connected to the liquid tank, the stirring device extends into the liquid tank, and the feeding trough communicates with the liquid tank.

5. The magnetic agarose microsphere production apparatus as described in claim 1, characterized in that, The liquid tank has a tank body, and electric heating elements are mounted around the side of the tank body.

6. The magnetic agarose microsphere production apparatus as described in claim 4, characterized in that, The stirring device includes a motor, a reducer, a support, a stirring shaft, and several stirring paddles. The support is installed in conjunction with the sealing cover, the reducer is installed in conjunction with the support, the motor is driven by the reducer, the stirring shaft is driven by the reducer, and several stirring paddles are installed on the stirring shaft, with the stirring paddles extending into the liquid tank.

7. The magnetic agarose microsphere production apparatus as described in claim 1, characterized in that, The top of the flow pool is connected to the outer atomizing box. The flow pool has an inclined surface inside. An outlet and an inlet are respectively opened on the side of the flow pool. The outlet and the inlet are respectively connected to the circulating cooling component through pipes. The inlet is located on the higher side of the flow pool inclined surface, and the outlet is located on the lower side of the flow pool inclined surface. A settling tank is set at the lowest end of the flow pool inclined surface. A discharge port is opened at the bottom of the settling tank. The bottom of the discharge port is connected to a receiving tank.

8. The magnetic agarose microsphere production apparatus as described in claim 7, characterized in that, The circulating refrigeration assembly includes a support frame, with the flow pool mounted on top of the support frame. The receiving tank, heat exchanger, and diaphragm pump are disposed inside the support frame. The inlet of the heat exchanger is connected to the outlet of the diaphragm pump via a pipe, the inlet of the diaphragm pump is connected to the water outlet via a pipe, and the outlet of the heat exchanger is connected to the water inlet via a pipe.

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