A method for improving seed germination rate and leaf growth of passion fruit
By soaking passion fruit seeds in a Ganoderma lucidum extract solution and spraying a mixture of Ganoderma lucidum extract and foliar fertilizer during the seedling stage, the problems of low germination rate and viral disease impact were solved, resulting in improved seed germination rate and healthy plant growth.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
- Filing Date
- 2023-11-23
- Publication Date
- 2026-06-26
AI Technical Summary
Passion fruit seeds have a hard outer shell, which leads to a low germination rate, especially for hybrid and mutant seeds. Furthermore, passion fruit virus diseases affect growth and fruit quality, and existing methods cannot effectively improve the germination rate or control the symptoms of viral diseases.
Soak passion fruit seeds in a Ganoderma lucidum solution for 20-28 hours before sowing. During the seedling stage, spray the plant leaves with a mixture of Ganoderma lucidum and foliar fertilizer. The ratio of Ganoderma lucidum to water is 1:1800-2200. Spray the mixture once every 10-15 days, for a total of ≥3 times.
It significantly improves the germination rate of passion fruit seeds, alleviates symptoms of viral diseases in plants, reduces pesticide use, promotes normal leaf growth, and enhances leaf vigor.
Smart Images
Figure CN117480896B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of planting technology, and in particular to a method for improving the germination rate of passion fruit seeds and the growth of leaves. Background Technology
[0002] Passion fruit seeds have a hard outer shell. Conventional methods of soaking the seeds in water and gibberellin result in low germination rates, especially for hybrid and mutant seeds, where the germination rate is even lower due to mutations.
[0003] Passion fruit virus disease not only severely affects the normal growth of passion fruit, but also leads to fruit deformities, reduced yield, poor quality, low economic benefits, and even total crop failure. It seriously impacts the production efficiency of passion fruit growers and enterprises, and is currently the most intractable problem for the sustainable development of the industry. At present, there are no drugs to cure the viral disease; the only recourse is to increase the application of water, fertilizer, and pesticides to promote plant growth and enhance plant resistance, thereby controlling or alleviating the symptoms of the viral disease and preventing harm to fruit quality and yield. Summary of the Invention
[0004] To address the aforementioned problems, this invention provides a method for improving the germination rate of passion fruit seeds and the vigor of leaf growth. The method provided by this invention not only improves the germination rate of passion fruit seeds but also significantly alleviates symptoms of viral diseases in the plant, reduces pesticide use, thereby reducing pesticide residues, and promotes normal leaf growth and vigor.
[0005] To achieve the above objectives, the present invention provides the following technical solution:
[0006] This invention provides a method for improving the germination rate of passion fruit seeds and the vigor of leaf growth, comprising the following steps:
[0007] Soak passion fruit seeds in a Ganoderma lucidum solution for 20–28 hours before sowing. During the seedling stage, spray a mixture of Ganoderma lucidum solution and foliar fertilizer onto the leaves of the passion fruit plants.
[0008] Preferably, the ganoderic acid solution comprises ganoderic acid and water; the volume ratio of ganoderic acid to water is 1:1800-2200.
[0009] Preferably, the sowing method includes: hill sowing; the hill sowing depth is 0.5-1cm; and the soil covering thickness for hill sowing is 0.3-0.5cm.
[0010] Preferably, the mixture is first sprayed when the passion fruit leaves show signs of passion fruit virus disease in the early or middle stage.
[0011] Preferably, when spraying the mixture at the initial stage of passion fruit virus disease on passion fruit leaves, the volume ratio of ganoderic acid to foliar fertilizer in the mixture is 1:1800-2200.
[0012] When applying foliar fertilizer during the mid-stage of passion fruit virus disease on passion fruit leaves, the volume ratio of Ganoderma lucidum extract to foliar fertilizer in the mixture should be 1:1400-1600.
[0013] Preferably, the mixture includes Ganoderma lucidum extract, water-soluble fertilizer containing medium-quantity elements, and water-soluble organic fertilizer.
[0014] Preferably, the method for preparing the mixture includes:
[0015] The organic water-soluble fertilizer is mixed with water to obtain an organic fertilizer solution; the volume ratio of the organic water-soluble fertilizer to water is 1:600-800.
[0016] The water-soluble fertilizer containing the medium-quantity elements and the organic fertilizer solution are mixed to obtain a foliar fertilizer; the volume ratio of the water-soluble fertilizer containing the medium-quantity elements to the organic fertilizer solution is 1:800-1000.
[0017] The ganoderic acid and the foliar fertilizer are mixed to obtain the mixture; the volume ratio of the ganoderic acid to the foliar fertilizer is 1:1400-2200.
[0018] Preferably, the spraying frequency is once every 10 to 15 days.
[0019] Preferably, the number of spraying applications is ≥3.
[0020] Beneficial effects:
[0021] This invention provides a method for improving the germination rate and leaf growth of passion fruit seeds, comprising the following steps: soaking passion fruit seeds in a ganoderic acid solution for 20-28 hours before sowing; and spraying a mixture of ganoderic acid and foliar fertilizer onto the leaves of the passion fruit plants during the seedling stage. This invention improves the germination rate of passion fruit seeds by soaking them in a ganoderic acid solution for an appropriate time; and significantly improves the symptoms of viral diseases by spraying the leaves of passion fruit plants with a mixture of ganoderic acid and foliar fertilizer, reducing pesticide use and pesticide residues, thus promoting normal leaf growth and improving leaf vigor. Attached Figure Description
[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.
[0023] Figure 1 The germination results of passionflower seeds in Example 1;
[0024] Figure 2 The germination results of passionflower seeds in Comparative Example 1;
[0025] Figure 3 Leaf growth before treatment with the mixed solution during the seedling stage;
[0026] Figure 4 The leaf growth after treatment with the mixed solution during the seedling stage;
[0027] Figure 5 Virus symptoms in plants before treatment with the mixed solution during the seedling stage;
[0028] Figure 6 Viral symptoms in plants treated with the mixed solution during the seedling stage;
[0029] Figure 7 To compare the leaf growth of seedlings treated with the mixed solution in Method 4;
[0030] Figure 8 The image shows the leaf growth after the seedlings were treated with the mixed solution in Example 1. Detailed Implementation
[0031] This invention provides a method for improving the germination rate of passion fruit seeds and the vigor of leaf growth, comprising the following steps:
[0032] Soak passion fruit seeds in a Ganoderma lucidum solution for 20–28 hours before sowing. During the seedling stage, spray a mixture of Ganoderma lucidum solution and foliar fertilizer onto the leaves of the passion fruit plants.
[0033] In this invention, the ganoderic acid solution preferably comprises ganoderic acid and water; the volume ratio of ganoderic acid to water is preferably 1:1800-2200, more preferably 1:2000; the soaking temperature is preferably 25-30℃, more preferably 30℃. The ganoderic acid used in this invention is preferably purchased from Langfang Weijin Agricultural Technology Co., Ltd., product name: EBE ganoderic acid. This invention improves the germination rate of passion fruit seeds by soaking them in the ganoderic acid solution for an appropriate time.
[0034] In this invention, the sowing method preferably includes: hill sowing; the hill sowing depth is preferably 0.5-1cm, more preferably 0.5cm; the hill sowing covering thickness is preferably 0.3-0.5cm, more preferably 0.5cm.
[0035] In this invention, the preferred time for the first application of the mixture is at the early or middle stage of the appearance of passion fruit virus disease on passion fruit leaves, more preferably at the early stage of the appearance of passion fruit virus disease on passion fruit leaves; when applying at the early stage of the appearance of passion fruit virus disease on passion fruit leaves, the volume ratio of ganoderic acid to foliar fertilizer in the mixture is preferably 1:1800-2200, more preferably 1:2000; when applying at the middle stage of the appearance of passion fruit virus disease on passion fruit leaves, the volume ratio of ganoderic acid to foliar fertilizer in the mixture is preferably 1:1400-1600, more preferably 1:1500; the mixture preferably includes ganoderic acid, water-soluble fertilizer containing medium-quantity elements, and water-soluble organic fertilizer. This invention does not have special requirements regarding the source of the water-soluble fertilizer containing medium-quantity elements and the water-soluble organic fertilizer; commercially available products well-known to those skilled in the art can be used. The medium-element water-soluble fertilizer used in this embodiment of the invention is preferably purchased from Shenzhen Dugao Biotechnology Co., Ltd., product name: Dugao Yougen Foliar Fertilizer Medium-element Water-soluble Fertilizer; the organic water-soluble fertilizer used in this embodiment of the invention is preferably purchased from Jiangmen Jieshi Plant Nutrition Co., Ltd., product name: Wujinlv Organic Water-soluble Fertilizer.
[0036] In this invention, the method for preparing the mixture preferably includes:
[0037] The organic water-soluble fertilizer is mixed with water to obtain an organic fertilizer solution;
[0038] The water-soluble fertilizer containing medium-quantity elements and the organic fertilizer solution are mixed to obtain a foliar fertilizer;
[0039] The Ganoderma lucidum extract and the foliar fertilizer are mixed to obtain the mixture.
[0040] In this invention, the volume ratio of the organic water-soluble fertilizer to water is preferably 1:600-800, more preferably 1:700; the volume ratio of the medium-element water-soluble fertilizer to the organic fertilizer solution is preferably 1:800-1000, more preferably 1:900; the volume ratio of the Ganoderma lucidum extract to the foliar fertilizer is preferably 1:1400-2200, more preferably 1:1500 or 1:2000.
[0041] In this invention, the spraying frequency is preferably once every 10 to 15 days, more preferably once every 10 days; the number of sprayings is preferably ≥3 times, more preferably ≥4 times.
[0042] This invention involves spraying Ganoderma lucidum extract and foliar fertilizer onto the leaves of passion fruit plants. Ganoderma lucidum extract is a microbial complex active polysaccharide substance that increases viscosity. By enhancing the plant's own photosynthetic efficiency, it promotes the absorption of external nutrients, which can significantly improve the symptoms of viral diseases in plants, reduce pesticide use, reduce pesticide residues, and allow passion fruit leaves to grow normally and improve leaf vigor.
[0043] To further illustrate the present invention, the following detailed description, in conjunction with the accompanying drawings and embodiments, describes a method for improving the germination rate and leaf growth of passion fruit seeds provided by the present invention, but these descriptions should not be construed as limiting the scope of protection of the present invention.
[0044] Example 1
[0045] A method for improving the germination rate and leaf growth of passion fruit seeds comprises the following steps:
[0046] 1) Soak sterilized potting soil (purchased from Sino-US joint venture Shandong Jiaston Fertilizer Co., Ltd.) in water for 1 day, fill it into the nutrient holes, occupying 4 / 5 of the holes, and poke small holes (0.5-1.0cm deep) with chopsticks. Soak passion fruit seeds in 2000 times diluted Ganoderma lucidum extract (BEB) at 30℃ for 24 hours, and then sow them in the holes in the potting soil. Cover with potting soil, with a thickness not exceeding 0.5cm, and water the potting soil thoroughly again. Shade and keep the potting soil moist every day.
[0047] 2) During the seedling stage, at the initial stage of passion fruit virus disease on passion fruit leaves, spray a mixture of Ganoderma lucidum extract and foliar fertilizer; the spraying frequency is once every 10 days; the number of sprayings is ≥3 times.
[0048] The preparation method of the mixture is as follows:
[0049] Organic water-soluble fertilizer and water are mixed to obtain an organic fertilizer solution; the preferred volume ratio of organic water-soluble fertilizer to water is 1:700.
[0050] A foliar fertilizer is obtained by mixing a water-soluble fertilizer containing medium-quantity elements and the organic fertilizer solution; the volume ratio of the water-soluble fertilizer containing medium-quantity elements to the organic fertilizer solution is 1:900.
[0051] Ganoderma lucidum extract and the foliar fertilizer are mixed to obtain the mixture; the volume ratio of the Ganoderma lucidum extract to the foliar fertilizer is 1:1500.
[0052] Comparative Example 1
[0053] A method similar to Example 1, except that step 1) is processed as follows:
[0054] Sterilized potting soil (purchased from Sino-US joint venture Shandong Jiaston Fertilizer Co., Ltd.) occupies 2 / 3 of the nutrient hole. Water it thoroughly beforehand. Soak passion fruit seeds in 2000 times diluted Ganoderma lucidum extract (BEB) at 25℃ for 24 hours, then sow them on the surface of the potting soil. Cover with potting soil to a thickness of 0.5-1.0cm, water it thoroughly again, and provide shade and keep the potting soil moist every day.
[0055] Test Example 1
[0056] Seeds from a hybrid of yellow-fruited passionflower and purple-fruited passionflower were treated according to step 1) of the methods in Example 1 and Comparative Example 1, respectively. Seed germination rates were observed and measured, and the results are shown below. Figure 1 and Figure 2 The seed germination rate of the method in Example 1 was 70%, while the seed germination rate of the method in Comparative Example 1 was 10%.
[0057] Depend on Figure 1 and Figure 2 It is evident that the method provided by this invention can significantly improve the germination rate of passion fruit seeds.
[0058] Test Example 2
[0059] Seedling stage observation Example 1 method before spraying the mixed solution ( Figure 3 ) and leaf growth 15 days after two applications ( Figure 4 ), the results are shown Figure 3 and Figure 4 .
[0060] Depend on Figure 3 and Figure 4 It can be seen that before the mixed solution was used to treat the seedlings, the leaves turned yellow, the chlorophyll content was low, and the growth was slow. After the mixed solution was used to treat the seedlings, the leaves turned green, the chlorophyll content was high, and the growth was healthy.
[0061] Test Example 3
[0062] Seedling stage observation Example 1 method before spraying the mixed solution ( Figure 5 The effect of spraying three times on the virus symptoms of plants 30 days later. Figure 6 ), the results are shown Figure 5 and Figure 6 .
[0063] Depend on Figure 5 and Figure 6 It can be seen that before the treatment with the mixed solution during the seedling stage, the plant showed obvious symptoms of viral disease, and the leaves were uneven. After the treatment with the mixed solution, the symptoms of viral disease in the plant were significantly improved, and the leaves grew normally.
[0064] The severity of foliar symptoms of viral diseases before and after spraying was evaluated. The severity of foliar symptoms of viral diseases was quantitatively evaluated as follows: 1 = no symptoms; 2 = mild mosaic, no leaf deformation; 3 = severe mosaic, no leaf deformation; 4 = severe mosaic, leaf blisters and deformation.
[0065] The results are as follows: Before the treatment with the mixed solution during the seedling stage, the severity of viral disease was 3-4, and after treatment, the severity of viral disease was 1-2.
[0066] Example 2
[0067] A method similar to Example 1, except that the soaking temperature is 25°C.
[0068] Comparative Example 2
[0069] A method similar to Example 2, except that the seeds are soaked in a 300 mg / L gibberellin (GA3) solution.
[0070] Comparative Example 3
[0071] A method similar to Example 2, except that the seeds are soaked in water.
[0072] Test Example 4
[0073] Different varieties of passion fruit seeds were treated according to the soaking methods of Example 2 and Comparative Examples 2-3, with different soaking times set. Each treatment was repeated 3 times, and the seed germination rate was observed and measured. The results are shown in Tables 1-4.
[0074] Table 1. Seed germination rate (%) under different treatments at Tainong Farms.
[0075]
[0076] Table 2. Germination rate (%) of Huangxiang No. 1 seeds under different treatments
[0077]
[0078] Table 3. Germination rates of different seeds under different treatments (200 seeds per treatment)
[0079]
[0080] Table 4 Seed germination rate under different treatments
[0081] Different treatments Interspecific hybridization 3 (Maternal parent: Baby's Breath, Male parent: Honey Gold) Soak in BEB (1:2000) at 15-20℃ for 24 hours. 5％～10％ Soak in 30℃ BEB (1:2000) for 24 hours 70％
[0082] As shown in Tables 1-4, the method provided by the present invention can improve the germination rate of passion fruit seeds.
[0083] Comparative Example 4
[0084] A method similar to Example 1, except that, during the seedling stage, at the initial stage of passion fruit virus disease on passion fruit leaves, a mixture of Ganoderma lucidum extract and NPK compound fertilizer is sprayed; the NPK compound fertilizer is purchased from Shenzhen Dugao Biotechnology Co., Ltd., and its trade name is water-soluble fertilizer with macro-elements, with a NPK content ratio of 20:20:20.
[0085] The preparation method of the mixture is as follows:
[0086] A compound fertilizer containing nitrogen, phosphorus, and potassium is mixed with water to obtain a compound fertilizer solution; the preferred mass-to-volume ratio of the compound fertilizer to water is 1 kg: 900 L.
[0087] Ganoderma lucidum extract and the compound fertilizer solution are mixed to obtain the mixture; the volume ratio of Ganoderma lucidum extract to the compound fertilizer solution is 1:1500.
[0088] Test Example 5
[0089] The leaf growth in Example 1 and Comparative Example 4 was observed 15 days after two applications of the method during the seedling stage. The results are shown in the figure. Figure 7 and Figure 8 .
[0090] Depend on Figure 7 and Figure 8 It can be seen that the plants treated by the method in Comparative Example 4 showed yellowing leaves and poor plant growth, while the yellowing leaves were significantly reduced in the method of this invention, the leaves gradually turned green, and the plant growth points were healthy.
[0091] Comparative Example 5
[0092] A method similar to Example 1, except that the mixture does not contain ganoderic acid.
[0093] The time for the leaves treated with Comparative Example 5 to return to healthy was extended to 2 months, and the leaves only turned green without any improvement in the symptoms of viral disease.
[0094] In summary, the method provided by this invention can not only improve the germination rate of passion fruit seeds, but also significantly improve the symptoms of viral diseases in plants, reduce pesticide use, reduce pesticide residues, and promote normal growth of passion fruit leaves, thereby improving leaf vigor.
[0095] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.
Claims
1. A method for improving the germination rate and leaf growth of passion fruit seeds, characterized in that, Includes the following steps: 1) Soak the sterilized potting soil in water for 1 day, then fill it into the nutrient holes, making up 4 / 5 of the holes. Use chopsticks to poke small holes 0.5-1.0 cm deep. Soak passion fruit seeds in a solution of 2000 times diluted Ganoderma lucidum extract at 25-30℃ for 24 hours, then sow them in the holes in the potting soil. Cover the seeds with potting soil, making sure the thickness is no more than 0.5 cm. Water the potting soil thoroughly again. Shade the soil and keep it moist every day. 2) During the seedling stage, at the initial stage of passion fruit virus disease on passion fruit leaves, spray a mixture of Ganoderma lucidum extract and foliar fertilizer; the spraying frequency is once every 10 days; the number of sprayings is ≥3 times. The preparation method of the mixture is as follows: Organic water-soluble fertilizer and water are mixed to obtain an organic fertilizer solution; the volume ratio of the organic water-soluble fertilizer to water is 1:
700. A foliar fertilizer is obtained by mixing a water-soluble fertilizer containing medium-quantity elements and the organic fertilizer solution; the volume ratio of the water-soluble fertilizer containing medium-quantity elements to the organic fertilizer solution is 1:
900. Ganoderma lucidum extract and the foliar fertilizer are mixed to obtain the mixture; the volume ratio of the Ganoderma lucidum extract to the foliar fertilizer is 1:1500.