Pure stem homogeneous tobacco leaf, compounded homogeneous tobacco leaf, preparation method and use thereof

Abstract

The application provides a pure stem homogeneous tobacco leaf, a compound homogeneous tobacco leaf, a preparation method and application thereof. The preparation method of the pure stem homogeneous tobacco leaf comprises the following steps: microbial fermentation, ultrasonic coupling enzyme extraction, stem extract preparation, stem extract quality improvement, homogeneous tobacco leaf base preparation, spice preparation and homogeneous tobacco leaf preparation. The application also discloses a compound homogeneous tobacco leaf prepared by using the pure stem homogeneous tobacco leaf. Through the processes of high-pressure steam sterilization, microbial fermentation, ultrasonic coupling enzyme extraction and quality improvement agent quality improvement, the application can effectively remove lignin, cellulose, pectin, protein and other macromolecules which are not conducive to sensory quality in the tobacco stem, make the taste comfortable and the smoke harmonious, increase the richness of tobacco aroma, make the aroma of the cigarette elegant, significantly improve the strength and satisfaction of the cigarette, replace part of the tobacco added to the cigarette, and fully utilize the tobacco stem to reduce the environmental pollution caused by the discharge.

Description

Technical Field

[0001] This invention relates to reconstituted tobacco technology, and more particularly to a pure stem homogenized tobacco leaf, a blended homogenized tobacco leaf, their preparation methods and uses. Background Technology

[0002] Homogenized tobacco sheets, also known as reconstituted tobacco sheets or tobacco leaf sheets, are mainly made from tobacco dust, fragments, stems, or low-grade tobacco leaves through deep processing. Homogenized tobacco sheets can be used as raw materials for tobacco products, replacing natural cigar wrappers, and are now widely used as cigarette raw materials. They can be blended with natural tobacco to produce cigarettes. Using low-grade tobacco raw materials to make sheets has advantages such as low cost, good filling properties, and reduced tar content in the smoke. It can improve the combustibility of cigarettes, reduce some harmful components in the smoke, lower tar and nicotine content, and improve the intrinsic quality of cigarette products.

[0003] Tobacco stems are a byproduct of the tobacco industry and also a valuable natural resource, accounting for approximately 25% to 30% of the weight of tobacco leaves. As a major tobacco producer, my country discards hundreds of thousands of tons of tobacco stems annually, causing environmental pollution and wasting natural resources.

[0004] Studies have shown that the components in tobacco stems are largely the same as those in tobacco leaves. However, tobacco stems contain lower levels of effective aroma components and higher levels of hemicellulose, lignin, pectin, and protein. Tobacco sheets made from tobacco stem extract have a strong off-flavor, weak aroma, poor aroma quality, strong spiciness, and an unpleasant aftertaste. Using them in tobacco sheet products severely impacts the sensory quality of the product and fails to meet the requirements of high-end products. Therefore, effectively degrading the biomolecules in tobacco stems is a significant challenge for the tobacco industry. Currently, domestic tobacco sheet manufacturers only use tobacco stem extract in small quantities in low-grade sheets, resulting in a large amount of stem extract being discharged into wastewater, causing significant resource waste and environmental pressure. If technical methods can be used to treat the stem extract to reduce its off-flavors and irritation, enhance its aroma, improve its aftertaste, further improve its sensory quality, expand its application range, increase its usage, and increase the utilization rate of tobacco stems, it will not only provide technical support for the formulation and application of tobacco stem extracts but also further improve the production yield of tobacco sheet products. This is of great significance for reducing raw material consumption, lowering production costs, and improving the economic benefits of enterprises.

[0005] From a socio-economic perspective, recycling large quantities of tobacco dust and short, broken stems into homogenized tobacco leaves is low-cost and saves a significant amount of natural tobacco leaves. This is beneficial for adjusting the rural industrial structure, conserving arable land, and alleviating the conflict between tobacco and grain production for land use. From an environmental protection perspective, it can effectively reduce the environmental harm caused by the disposal of tobacco dust, pollutants, and broken stems. Summary of the Invention

[0006] The purpose of this invention is to address the problem of varying degrees of sensory quality defects in tobacco stems, stem extract, or products, and the issue that their use in tobacco sheet products severely affects the sensory quality of the products. This invention proposes a method for preparing pure stem homogenized tobacco leaves. This method utilizes processes such as high-pressure steam sterilization, microbial fermentation, ultrasonic-coupled enzymatic extraction, and quality enhancement agents to effectively remove large molecules in tobacco stems that are detrimental to sensory quality, such as lignin, cellulose, pectin, and protein. This results in a more pleasant taste, harmonious smoke, increased aroma richness, and an elegant cigarette aroma. It also significantly improves the strength and satisfaction of cigarettes, and can replace some tobacco leaves in cigarette production. Furthermore, the full utilization of tobacco stems reduces environmental pollution caused by emissions.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is: a method for preparing homogeneous tobacco leaves with pure stems, comprising the following steps:

[0008] (1) Microbial fermentation: Microorganisms are sprayed onto sterilized tobacco stems for fermentation;

[0009] (2) Ultrasonic coupled enzyme extraction: The tobacco stems after microbial fermentation were extracted by ultrasonic coupled enzyme method. After extraction, solid-liquid separation was performed to obtain stem residue and tobacco stem extract.

[0010] (3) Preparation of stem extract: Stem extract was obtained by vacuum concentration of tobacco stem extract;

[0011] (4) Stem extract quality improvement: First, mix the first quality improver with the stem extract and cook at high temperature. After cooling, mix the second quality improver with the cooked stem extract and let it stand to clarify to obtain the improved stem extract.

[0012] (5) Preparation of homogeneous tobacco leaf substrate: The stem residue is dried, homogenized with water, and then pulped. The homogeneous tobacco leaf substrate is obtained by processing it through a paper forming machine. The purpose of drying the stem residue is to remove residual stem liquid, so as to facilitate rapid determination of the moisture content of the stem residue, accurately control the pulping, pulping and subsequent substrate processing, and improve the substrate qualification rate.

[0013] (6) Flavoring preparation: The flavoring is obtained by mixing tobacco extract, plant extract, refined stem extract and water.

[0014] (7) Preparation of homogeneous tobacco leaves: Pure stem homogeneous tobacco leaves are prepared by mixing spices with homogeneous tobacco leaf substrate.

[0015] Further, the tobacco stems mentioned in step (1) are composite tobacco stems, which include, but are not limited to, one or more of Zunyi tobacco stems, Guiyang tobacco stems, Bijie tobacco stems, Qianxinan tobacco stems, and Yunnan tobacco stems. Preferably, the composite tobacco stems include Zunyi tobacco stems, Guiyang tobacco stems, Bijie tobacco stems, Qianxinan tobacco stems, and Yunnan tobacco stems in a mass ratio of 10-20%: 5-10%: 15-20%: 25-30%: 20-25%.

[0016] Furthermore, the length of the tobacco stem is 2-4 cm.

[0017] Further, the tobacco stem raw material is pretreated before step (1): cutting the stems: the tobacco stems are cut into lengths of 0.5-1cm; sieving to remove impurities: the cut tobacco stems are sieved by vibration to remove broken stems and impurities; washing and sterilizing the stems: the cut tobacco stems are soaked in water to rinse and remove the broken pieces and are filtered dry, and then sterilized by high pressure steam to make the tobacco stems soft, which is convenient for subsequent fermentation.

[0018] Furthermore, the high-pressure steam sterilization is performed at a steam pressure of 100-105 kPa, a time of 15-20 min, and a temperature of 120-122 °C.

[0019] Furthermore, the microorganisms mentioned in step (1) include, but are not limited to, one or more combinations of Aspergillus niger, wood-rotting fungi (such as white rot fungi, soft rot fungi, or brown rot fungi), and Penicillium micropurpurum.

[0020] Further, the amount of microorganisms applied in step (1) is 0.1-4%. Preferably, when the microorganisms are Aspergillus niger, wood-rotting fungi, and Penicillium purpureus, the application amounts are 0.1-0.2%, 1-1.5%, and 1-2% of the tobacco stem mass, respectively. The simultaneous use of Aspergillus niger, wood-rotting fungi, and Penicillium purpureus can produce a unique aroma while decomposing cellulose, lignin, and pectin.

[0021] Furthermore, the fermentation temperature in step (1) is 32-37℃ and the time is 18-24h.

[0022] Furthermore, the enzyme described in step (2) is a biomimetic enzyme, including but not limited to one or more combinations of protein hydrolysis enzymes, lignin hydrolysis enzymes, pectin hydrolysis enzymes, and polysaccharide hydrolysis enzymes.

[0023] Further, the amount of biomimetic enzyme added in step (2) is 0.01-0.20%. When the preferred biomimetic enzymes are protein hydrolysing enzymes, lignin hydrolysing enzymes, pectin hydrolysing enzymes, and polysaccharide mimics, the added amounts are 0.01-0.03%, 0.08-0.10%, 0.05-0.10%, and 0.03-0.05% of the tobacco stem mass, respectively. The use of a composite biomimetic enzyme of protein hydrolysing enzymes, lignin hydrolysing enzymes, pectin hydrolysing enzymes, and polysaccharide mimics can better remove cell wall substances from tobacco stems, reduce the content of pectin, cellulose, lignin, and protein in tobacco stems, and improve the smoking quality of homogeneous tobacco leaves.

[0024] Further, in step (2), the extraction solvent is water, the material-to-liquid ratio is 1:9-10, the extraction temperature is 75-100℃, the ultrasonic frequency is 60-90Hz, and the extraction time is 30-40min.

[0025] Furthermore, the vacuum concentration temperature in step (3) is 50-70℃, and the vacuum degree is 0.05-0.07Mpa.

[0026] Furthermore, the solid content of the extract in step (3) is 35-45%. Unless otherwise specified, all percentages in this invention are by mass.

[0027] Further, in step (4), the first improving agent is one or more of glycerol, KOH and NaOH; the second improving agent is one or more of bentonite, kaolin, cationic resin (PPVP) and other materials.

[0028] Further, in step (4), the amount of the first quality enhancer is 35-40% of the mass of the stem extract. Preferably, when the first quality enhancer is glycerol, KOH, or NaOH, the application amounts are 20-30%, 5-10%, and 5-10% of the mass of the stem extract, respectively. The first quality enhancer is a combination of glycerol and alkali, which can further reduce the lignin content and improve the quality of flue gas.

[0029] Further, in step (4), the amount of the second quality enhancer is 30-40% of the mass of the stem extract. Preferably, when the second quality enhancer is bentonite, kaolin, and cationic resin (PPVP), the application amounts are 10-20%, 5-10%, and 5-10% of the mass of the stem extract, respectively. The second quality enhancer uses a combination of bentonite, kaolin, and cationic resin (PPVP) as adsorbents, which can better adsorb residual proteins and other macromolecular impurities in the stem extract, further improving off-flavors and aftertaste.

[0030] Furthermore, the high-temperature cooking time in step (4) is 0.5-1.0h.

[0031] Furthermore, the settling and clarification time in step (4) is 25-30 minutes.

[0032] Furthermore, the thickness of the homogeneous tobacco substrate in step (5) is 0.18-0.22 mm.

[0033] Further, the tobacco extract in step (6) is tobacco paste and / or tobacco puree; the plant extract is plant essential oil and / or plant powder.

[0034] Furthermore, in step (6), the proportions of tobacco extract, plant extract, refined stem extract, and water added are 6.5-8.5%, 1.5-2.5%, 85-90%, and 3-5%, respectively.

[0035] Further, the method for preparing homogenized tobacco leaves in step (7):

[0036] The spices are evenly applied to the homogeneous tobacco leaf base, dried, and shredded to obtain pure stem homogeneous tobacco leaves.

[0037] Alternatively, after slicing homogeneous tobacco leaf substrate into shreds, the flavoring is evenly sprayed onto the homogeneous tobacco leaf substrate shreds to obtain pure stem homogeneous tobacco leaves.

[0038] Furthermore, the amount of the flavoring applied is 10-12% of the mass of the homogeneous tobacco leaf substrate.

[0039] Furthermore, the shred width is 0.7-1.1 mm.

[0040] Another objective of this invention is to disclose a homogeneous tobacco leaf with pure stems, prepared using the method described above.

[0041] Another object of the present invention is to disclose the application of pure stem homogenized tobacco leaves in the field of tobacco leaf formulation.

[0042] Another object of the present invention discloses a compound homogeneous tobacco leaf, comprising pure stem homogeneous tobacco leaves and natural tobacco leaves in a mass ratio of 5:5 to 3:7.

[0043] Another objective of this invention is to disclose a method for preparing blended homogeneous tobacco leaves, comprising the following steps: blending pure stem homogeneous tobacco leaves with natural tobacco leaves to obtain blended homogeneous tobacco leaves.

[0044] Alternatively, pure stem homogeneous tobacco leaves can be pulverized to prepare tobacco powder, and cationic guar gum can be added and sprayed onto natural tobacco leaves to obtain compound homogeneous tobacco leaves.

[0045] The present invention relates to pure stem homogeneous tobacco leaves, compound homogeneous tobacco leaves, their preparation methods, and applications, which have the following advantages compared with the prior art:

[0046] (1) The pure stem homogeneous tobacco leaves prepared by this invention have high strength, significantly higher tensile strength than conventional homogeneous tobacco leaves, low dusting rate, strong plasticity during processing, and high processability.

[0047] (2) This invention uses high-pressure steam sterilization (sterilization and softening of tobacco stems), microbial fermentation (enhancing aroma, decomposing cellulose, lignin, and pectin), ultrasonic coupled enzyme extraction (reducing pectin, cellulose, lignin, and protein in tobacco stems), and quality improvement with quality enhancers (reducing lignin and protein, improving aftertaste and off-flavors) to effectively remove large molecules such as lignin, cellulose, pectin, and protein from tobacco stems that are detrimental to sensory quality, making the taste comfortable, the smoke harmonious, and increasing the richness of the tobacco aroma, making the aroma of cigarettes elegant, and significantly improving the strength and satisfaction of cigarettes. It can replace some tobacco leaves added to cigarettes, and the full utilization of tobacco stems can reduce environmental pollution caused by external discharge.

[0048] (3) The enzyme preparation of the present invention does not need to be inactivated after the reaction.

[0049] (4) This invention can enhance the strength of homogeneous tobacco leaves, improve production continuity, and is easy to control and operate.

[0050] (4) Make full use of tobacco stem raw materials and water extracts to reduce environmental pollution and save costs. Detailed Implementation

[0051] The present invention will be further described below with reference to the embodiments:

[0052] Example 1

[0053] This embodiment discloses a method for preparing homogeneous tobacco leaves with pure stems, the specific steps of which are as follows:

[0054] (1) Pretreatment of tobacco stem raw materials: Take 20% Zunyi tobacco stems, 10% Guiyang tobacco stems, 20% Bijie tobacco stems, 30% Qianxinan tobacco stems and 20% Yunnan tobacco stems with a length of 4cm, mix them evenly, cut them to a length of 1.0cm, remove broken stems and impurities by vibrating sieve after cutting, soak and rinse with water to remove debris and filter dry, then sterilize with high pressure steam for 20min under steam pressure of 100Kpa and temperature of 120℃, so as to make the tobacco stems soft and easy for subsequent fermentation.

[0055] (2) Microbial fermentation: 0.1% of Aspergillus niger, 1.0% of wood-rotting fungi and 2.0% of Penicillium micropurpurum by weight of tobacco stems were diluted with water and sprayed evenly on sterilized tobacco stems, and fermented at 35℃ for 24 hours.

[0056] (3) Ultrasonic coupling enzyme extraction: The tobacco stems after microbial fermentation were extracted at 75℃ and 75Hz for 30 min with 0.01% hydrolyzed protein mimic enzyme, 0.08% hydrolyzed lignin mimic enzyme, 0.05% hydrolyzed pectin mimic enzyme and 0.05% hydrolyzed polysaccharide mimic enzyme by weight of tobacco stems. After extraction, solid and liquid separation was performed to obtain stem residue and tobacco stem extract.

[0057] (4) Preparation of stem extract: The tobacco stem extract was concentrated under reduced pressure at a temperature of 65℃ and a vacuum degree of 0.07Mpa to obtain a stem extract with a solid content of 35%.

[0058] (5) Stem extract quality improvement: First, mix the first quality improvement agent (20% of the stem extract mass of glycerol, 10% of KOH, and 10% of NaOH) with the stem extract and cook at 100°C for 30 minutes. After cooling, mix the second quality improvement agent (10% of the stem extract mass of bentonite, 10% of kaolin, and 10% of cationic resin ppvp) with the cooked stem extract and let it stand for clarification for 25 minutes to obtain the improved stem extract.

[0059] (6) Preparation of homogeneous tobacco leaf substrate: The stem residue is dried, homogenized with water, and then ground into pulp. The pulp is then processed by a paper forming machine to obtain homogeneous tobacco leaf substrate.

[0060] (7) Flavoring preparation: Mix 8.5% tobacco extract, 1.5% plant essential oil, 85% refined stem extract and 5% water to obtain the flavoring;

[0061] (8) Preparation of homogeneous tobacco leaves: The spices are evenly applied on the homogeneous tobacco leaf base with a thickness of 0.18 mm at 10% of the mass of the homogeneous tobacco leaf base. After drying, the pure stem homogeneous tobacco leaves with a width of 0.8 mm are obtained after passing through a 0.8 mm cutter.

[0062] Example 2

[0063] This embodiment discloses a method for preparing homogeneous tobacco leaves with pure stems, the specific steps of which are as follows:

[0064] (1) Pretreatment of tobacco stem raw materials: Take 20% Zunyi tobacco stems, 10% Guiyang tobacco stems, 15% Bijie tobacco stems, 30% Qianxinan tobacco stems and 25% Yunnan tobacco stems with a length of 2cm, mix them evenly, cut them to a length of 0.5cm, remove broken stems and impurities by vibrating sieve after cutting, soak and rinse with water to remove debris and filter dry, then sterilize with high pressure steam for 15min under steam pressure of 105Kpa and temperature of 122℃, so as to make the tobacco stems soft and easy for subsequent fermentation.

[0065] (2) Microbial fermentation: 0.2% of Aspergillus niger, 1.5% of wood-rotting fungi and 2.0% of Penicillium micropurpurum by weight of tobacco stems were diluted with water and sprayed evenly on sterilized tobacco stems, and fermented at 37°C for 18 hours.

[0066] (3) Ultrasonic coupling enzyme extraction: The tobacco stems after microbial fermentation were extracted at 75℃ and 75Hz for 30 min with 0.03% hydrolyzed protein mimic enzyme, 0.10% hydrolyzed lignin mimic enzyme, 0.07% hydrolyzed pectin mimic enzyme and 0.03% hydrolyzed polysaccharide mimic enzyme by weight of tobacco stems. After extraction, solid and liquid separation was performed to obtain stem residue and tobacco stem extract.

[0067] (4) Preparation of stem extract: The tobacco stem extract was concentrated under reduced pressure at a temperature of 70°C and a vacuum degree of 0.05 MPa to obtain a stem extract with a solid content of 40%.

[0068] (5) Stalk extract quality improvement: First, mix the first quality improvement agent (25% of the stalk extract mass of glycerol, 5% of KOH, and 5% of NaOH) with the stalk extract and cook at 90°C for 45 minutes. After cooling, mix the second quality improvement agent (20% of the stalk extract mass of bentonite, 5% of kaolin, and 5% of cationic resin ppvp) with the cooked stalk extract and let it stand for clarification for 30 minutes to obtain the improved stalk extract.

[0069] (6) Preparation of homogeneous tobacco leaf substrate: The stem residue is dried, homogenized with water, and then ground into pulp. The pulp is then processed by a paper forming machine to obtain homogeneous tobacco leaf substrate.

[0070] (7) Flavoring preparation: Mix 6.5% tobacco oil, 2.5% plant powder, 88% refined stem extract and 3% water to obtain the flavoring;

[0071] (8) Preparation of homogeneous tobacco leaves: After passing a homogeneous tobacco leaf substrate with a thickness of 0.22 mm through a 1.0 mm cutter, pure stem homogeneous substrate shreds with a width of 1.0 mm are obtained. The spices are evenly sprayed onto the substrate shreds at a ratio of 12% of the mass of the homogeneous tobacco leaf substrate to obtain pure stem homogeneous tobacco leaves.

[0072] Example 3

[0073] This embodiment discloses a method for preparing compound homogeneous tobacco leaves, the specific steps of which are as follows:

[0074] The homogeneous tobacco leaves from Example 1 were blended with natural tobacco leaves at a mass ratio of 5:5 and then mixed to obtain blended homogeneous tobacco leaves.

[0075] Example 4

[0076] This embodiment discloses a method for preparing compound homogeneous tobacco leaves, the specific steps of which are as follows:

[0077] The homogeneous tobacco leaves from Example 2 were pulverized to prepare tobacco powder, and 0.05% cationic guar gum was added and evenly sprayed onto natural tobacco leaves. After drying, compound homogeneous tobacco leaves were obtained.

[0078] Sensory evaluations were conducted on the pure stem homogeneous tobacco leaves and blended homogeneous tobacco leaves from the above embodiments, and the specific results are shown in Table 1. Nine intermediate and senior judges were invited to conduct the sensory evaluation, using a cyclical evaluation method. The total score was set at 100 points, and the final score was the average after removing the highest and lowest scores.

[0079] Table 1 Sensory Evaluation Table

[0080]

[0081]

[0082] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing pure stem homogenized tobacco leaves, characterized by, It comprises the following steps: (1) Washing and sterilizing: after cutting, the tobacco stem is soaked in water to remove the dust and then filtered and dried. Then, the tobacco stem is sterilized by high-pressure steam to make it soft and facilitate subsequent fermentation. (2) Ultrasonic coupling enzyme extraction: the tobacco stem after microbial fermentation is subjected to ultrasonic coupling enzyme extraction, and then solid-liquid separation is performed to obtain stem residue and tobacco stem extract; (3) Preparation of stem paste: the tobacco stem extract is reduced-pressure concentrated to obtain stem paste; (4) Stem paste quality improvement: the first quality improvement agent is mixed with the stem paste, and high-temperature cooking is performed. After cooling, the second quality improvement agent is mixed with the cooked stem paste, and then standing and clarification are performed to obtain the quality-improved stem paste. The first quality improvement agent is a combination of one or more of glycerol, KOH and NaOH. The second quality improvement agent is a combination of one or more of bentonite, kaolin and cationic resin. The amount of the first quality improvement agent is 35-40% of the mass of the stem paste, and the amount of the second quality improvement agent is 30-40% of the mass of the stem paste; (5) Preparation of homogeneous tobacco leaf base: the stem residue is dried, added with water and homogenized, and then milled to obtain a homogeneous tobacco leaf base; (6) Preparation of flavor: tobacco extract, plant extract, quality-improved stem paste and water are mixed to obtain a flavor; The addition proportions of the tobacco extract, plant extract, quality-improved stem paste and water are 6.5-8.5%, 1.5-2.5%, 85-90% and 3-5%, respectively; (7) Preparation of homogeneous tobacco leaf: the flavor and the homogeneous tobacco leaf base are mixed to obtain pure stem homogeneous tobacco leaf. In step (2), the enzyme is a combination of one or more of protease, ligninase, pectinase and polysaccharide enzyme. The extraction solvent is water, the solid-liquid ratio is 1:9-10, the extraction temperature is 75-100°C, the ultrasonic frequency is 60-90 Hz, and the extraction time is 30-40 min.

2. The method of claim 1, wherein the pure stem homogeneous tobacco leaf is prepared by the steps of: In step (3), the reduced-pressure concentration temperature is 50-70°C, and the vacuum degree is 0.05-0.07 MPa. ​ 3. The method of claim 1, wherein the pure stem homogeneous tobacco leaf is prepared by the steps of: In step (6), the tobacco extract is tobacco alcohol paste and / or tobacco absolute, and the plant extract is plant essential oil and / or plant dry powder. ​ 4. The method of claim 1, wherein the pure stem homogeneous tobacco leaf is prepared by the steps of: The preparation method of the homogeneous tobacco leaf in step (7) is as follows: ​ The flavor is evenly applied on the homogeneous tobacco leaf base, dried and cut into shreds to obtain pure stem homogeneous tobacco leaf. Or, the homogeneous tobacco leaf base is cut into shreds, and the flavor is evenly sprayed on the shreds to obtain pure stem homogeneous tobacco leaf.

5. A pure stem homogeneous tobacco leaf, characterized by, The pure stem homogeneous tobacco leaf is prepared by the method of any one of claims 1-4.

6. A compounded homogenized tobacco leaf characterized in that, The pure stem homogeneous tobacco leaf comprises the pure stem homogeneous tobacco leaf of claim 5 and natural tobacco leaf in a mass ratio of 5:5-3:

7.

7. A method of preparing the reconstituted homogenized tobacco leaf of claim 6, wherein, The method comprises the following steps: The pure stem homogeneous tobacco leaf is mixed with natural tobacco leaf to obtain mixed homogeneous tobacco leaf. Or, the pure stem homogeneous tobacco leaf is pulverized into tobacco powder, and the tobacco powder is sprayed on the natural tobacco leaf to obtain mixed homogeneous tobacco leaf.

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