A composition containing a plant polypeptide and a method for preparing and using the same

Abstract

The present application belongs to the technical field of cosmetics, and discloses a composition containing plant polypeptides, a preparation method and application thereof.The composition containing plant polypeptides comprises plant polypeptides, kava leaf / root / stem extract and grapevine extract; the plant polypeptides comprise mung bean polypeptides, pea polypeptides and red bean polypeptides.The present application utilizes the moisturizing property and strong antioxidant property of plant polypeptides and grapevine extract, and combines the kava leaf / root / stem extract with good soothing performance, so that the composition has good moisturizing, soothing, firming and anti-wrinkle effects through the synergistic effect of removing free radicals, promoting the synthesis of collagen and elastin and soothing nerves, meets the needs of consumers for various skin care effects, is mild and non-irritating, and can fully utilize the abandoned grapevine resources, has certain environmental protection significance and broad market prospect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a plant polypeptide-containing composition and a preparation method and application thereof. BACKGROUND

[0002] Functional ingredients in cosmetics can be divided into natural ingredients and chemical ingredients. Studies have found that, compared with natural ingredients, chemical ingredients often have some harmful or irritating problems. With the gradual enhancement of public safety and environmental protection awareness, and the safety problems easily caused by chemical ingredient raw materials, whether the cosmetic raw materials are natural, green, healthy and safe has increasingly attracted the attention of consumers. At present, most plant active ingredients are simply directly added in cosmetics without considering the interaction between different ingredients, resulting in some problems such as difficulty in being absorbed by the skin, single or insignificant efficacy, high irritation and the like.

[0003] Therefore, it is very necessary to develop a product with lower or no irritation and obvious multiple skin care effects. SUMMARY

[0004] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a plant polypeptide-containing composition and a preparation method and application thereof. The plant polypeptide-containing composition plays a synergistic effect by reasonably compounding several plant active ingredients, is mild and non-irritating, and has good antioxidant, moisturizing, soothing, firming and anti-wrinkle multiple skin care effects.

[0005] The present application provides a composition in the first aspect, the composition comprising: plant polypeptide, kava leaf / root / stem extract and grapevine extract; the plant polypeptide comprising: mung bean polypeptide, pea polypeptide and red bean polypeptide.

[0006] Specifically, various structures of bioactive peptides composed of a plurality of amino acids isolated from plants in different compositions and arrangements are called plant polypeptides. Plant polypeptides are widely sourced, highly safe, low in extraction cost, and have a variety of human metabolic and physiological regulation functions. Applying them to cosmetics has broad application prospects and value. Legumes are an important source of active peptides due to their high-quality protein content. Mung beans, peas and red beans are common grain legumes and are natural and high-quality sustainable protein sources. The raw materials are easy to obtain and the cost is relatively low. The present application compounding mung bean polypeptide, pea polypeptide and red bean polypeptide has excellent moisturizing effect. In addition, it also has strong antioxidant and the ability to reduce the mRNA expression of cyclooxygenase-2 (COX-2), thereby improving skin cell damage and aging caused by excessive free radicals and inflammatory reactions.

[0007] Kava (Piper methysticum Forst), commonly known as kava, is a wild shrub plant in the South Pacific islands. Kava lactones are the main active molecules in kava, mainly including methysticin, dihydromethysticin, methoxymethysticin, narcotic pepper and desmethoxymethysticin. A variety of kava lactones are essential for the synergistic effect in the soothing mechanism. Kava can soothe nerves and have an immediate calming effect by affecting the binding of GABA and GABA receptor, reducing the production of TRPV1 receptor protein and combining with CB1 receptor. Studies have shown that the biological activity of the whole extract of kava is higher than that of the extract of single kava lactone. The kava leaf / root / stem extract used in the present application can more effectively reduce skin redness and improve stinging.

[0008] Grapevines are plants with medicinal and edible properties. Studies have found that grapevines contain a large amount of functional substances such as resveratrol, tannin, flavone, proanthocyanidin and vitamin. The grapevines not only have the effects of scavenging free radicals, antioxidant and delaying cell aging, but also can promote the synthesis of collagen and elastin, inhibit the decomposition of elastase and tighten the skin. In order to improve grape yield, a large amount of grapevine branches and tendrils need to be pruned and incinerated, which causes pollution of ecological environment and serious waste of resources. The grapevine extract used in cosmetics can make full use of resources and has the above skin care effects.

[0009] By using the above technical scheme, the plant polypeptide and the grapevine extract are used to have the effects of moisturizing and strong antioxidant, and the kava leaf / root / stem extract is used to have good soothing performance, so that the composition has good effects of moisturizing, soothing, tightening and anti-wrinkle by the synergistic effect of scavenging free radicals, promoting the synthesis of collagen and elastin and soothing nerves, meets the needs of consumers for various skin care effects, is mild and non-irritating, and can make full use of the abandoned grapevine resources, has certain environmental protection significance and broad market prospect.

[0010] According to some embodiments of the present application, the composition comprises, in terms of mass fraction, 1-15 parts of mung bean polypeptide, 1-15 parts of pea polypeptide, 1-15 parts of red bean polypeptide, 2-10 parts of kava leaf / root / stem extract and 5-20 parts of grapevine extract.

[0011] According to some embodiments of the present application, the composition comprises, in terms of mass fraction, 1-12 parts of mung bean polypeptide, 1-12 parts of pea polypeptide, 1-12 parts of red bean polypeptide, 2-9 parts of kava leaf / root / stem extract and 5-18 parts of grapevine extract.

[0012] According to some embodiments of the present application, the composition comprises, in terms of mass fraction, 1-10 parts of mung bean polypeptide, 1-10 parts of pea polypeptide, 1-10 parts of red bean polypeptide, 2-8 parts of piper methysticum leaf / root / stem extract, and 5-15 parts of grape vine extract.

[0013] According to some embodiments of the present application, the composition further comprises a solvent; the solvent comprises water and a polyhydric alcohol.

[0014] According to some embodiments of the present application, the water is added in the composition in an amount of 20-90 parts by mass fraction; the polyhydric alcohol is added in the composition in an amount of 0.1-2 parts by mass fraction. Further, the water is added in the composition in an amount of 20-85 parts by mass fraction. Further, the water is added in the composition in an amount of 20-80 parts by mass fraction.

[0015] According to some embodiments of the present application, the polyhydric alcohol comprises at least one of 1,2-hexanediol, glycerol, propylene glycol, butanediol, 1,2-pentanediol, and 1,3-propanediol.

[0016] The second aspect of the present application provides a preparation method of the composition of the present application, comprising the following steps:

[0017] The plant polypeptide, the piper methysticum leaf / root / stem extract, and the grape vine extract are mixed to obtain the composition.

[0018] According to some embodiments of the present application, the preparation method of the plant polypeptide comprises the following steps:

[0019] a) crushing a plant raw material, mixing the crushed plant raw material with water, wherein the solid-liquid ratio is 1:(10-15) m / m, then ultrasonic-assisted extraction is performed at 35-45℃ and pH 8.5-9.5 for 20-30 min, followed by centrifugation at 4500-5500 r / min for 8-12 min, taking the supernatant, adjusting the pH to 4.0-5.0, and collecting the precipitate after centrifugation again, washing the precipitate with water, and freeze-drying to obtain a plant protein freeze-dried powder for standby;

[0020] b) weighing the plant protein freeze-dried powder obtained in step a) to prepare a protein solution (4.5-5.5%, m / m), adding an enzyme for enzymolysis, and the enzymolysis conditions include: temperature 50-60℃, pH 6.0-12.0, enzyme amount 3000-8000 U, substrate concentration 4.0-5.0%, and time 3.5-4.5 h, after complete enzymolysis, heating to 100℃ for 3-6 min for enzyme inactivation, centrifugation, and taking the supernatant, to obtain the plant polypeptide.

[0021] Further, the pH of enzymolysis is 8.5-9.5, and the enzyme amount is 4500-6000 U; by optimizing the pH and enzyme amount of enzymolysis, the yield of plant polypeptide is further improved, which can reach more than 20%.

[0022] According to some embodiments of the present application, the enzyme is selected from papain.

[0023] The third aspect of the present application provides a cosmetic product comprising the composition of the present application.

[0024] According to some embodiments of the present application, the amount of the composition added to the cosmetic product is 1-20 wt%.

[0025] According to some embodiments of the present application, the amount of the composition added to the cosmetic product is 5-15 wt%.

[0026] According to some embodiments of the present application, the dosage form of the cosmetic product is selected from cream, emulsion, water, gel or mask.

[0027] The fourth aspect of the present application provides an essence comprising the composition of the present application.

[0028] Compared with the prior art, the present application has the following advantages:

[0029] The present application reasonably compounds mung bean polypeptide, pea polypeptide, red bean polypeptide, kava leaf / root / stem extract and grape vine extract, and the components play a synergistic effect, are mild and non-irritating, and have good antioxidant, moisturizing, soothing, firming and anti-wrinkle multiple skin care effects. DETAILED DESCRIPTION

[0030] The content of the present application is further described in detail through specific examples. Unless otherwise specified, the raw materials, reagents or devices used in the examples can be obtained from conventional commercial channels or can be obtained by existing technical methods. Unless otherwise specified, the test or test method is a conventional method in the art.

[0031] Example 1

[0032] A plant polypeptide-containing composition comprises the following components in mass fraction:

[0033] Deionized water 57 parts, mung bean polypeptide 8.5 parts, pea polypeptide 8 parts, red bean polypeptide 7.5 parts, kava leaf / root / stem extract 5.5 parts, grape vine extract 12.5 parts, 1,2-hexanediol 1 part.

[0034] The preparation method of the above plant polypeptide-containing composition comprises the following steps:

[0035] (1) Preparation of plant polypeptide:

[0036] a) separately crushing mung bean, pea, and red bean raw materials, taking an appropriate amount of raw materials and mixing with deionized water (the ratio of material to liquid is 1:15, m / m), ultrasonic-assisted extraction at 40°C and pH 9.0 for 25 min, then centrifuging at 4500-5500 r / min for 10 min, taking the supernatant, adjusting the pH to 4.5, collecting the precipitate after centrifugation again, washing the precipitate with water, and freeze-drying to obtain plant protein freeze-dried powder for use;

[0037] b) taking the plant protein freeze-dried powder obtained in step a) to prepare a protein solution (5.0%, m / m), placing it in an enzyme reactor, adding enzymes for enzymolysis, adjusting the enzymolysis temperature, pH, enzyme amount, substrate concentration, and enzymolysis time according to different plant raw materials as shown in Table 1, heating to 100°C after complete enzymolysis for 5 min to inactivate the enzymes, centrifuging, and taking the supernatant to obtain mung bean polypeptide, pea polypeptide, and red bean polypeptide, respectively.

[0038] (2) mixing and stirring deionized water, mung bean polypeptide, pea polypeptide, red bean polypeptide, kava leaf / root / stem extract, and grapevine extract uniformly;

[0039] (3) adding 1,2-hexanediol to the mixture obtained in step (2) and stirring uniformly to obtain a plant polypeptide-containing composition.

[0040] Table 1 Enzymolysis conditions of different plant polypeptides

[0041]

[0042]

[0043] Note: The enzyme used in the experiment is papain.

[0044] Example 2

[0045] A plant polypeptide-containing composition includes the following components in mass fractions:

[0046] deionized water 61 parts, mung bean polypeptide 7 parts, pea polypeptide 6 parts, red bean polypeptide 7 parts, kava leaf / root / stem extract 3 parts, grapevine extract 15 parts, and 1,2-hexanediol 1 part.

[0047] Example 3

[0048] A plant polypeptide-containing composition includes the following components in mass fractions:

[0049] deionized water 57.5 parts, mung bean polypeptide 8 parts, pea polypeptide 8 parts, red bean polypeptide 8 parts, kava leaf / root / stem extract 6 parts, grapevine extract 11.5 parts, and 1,2-hexanediol 1 part.

[0050] Comparative Example 1

[0051] Comparative Example 1 differs from Example 1 in that the composition of Comparative Example 1 does not add a plant active ingredient, and the composition includes the following components in mass parts: deionized water 99 parts, 1,2-hexanediol 1 part.

[0052] Comparative Example 2

[0053] Comparative Example 2 differs from Example 1 in that the composition of Comparative Example 2 only adds one plant active ingredient, and the composition includes the following components in mass parts: deionized water 90.5 parts, mung bean polypeptide 8.5 parts, 1,2-hexanediol 1 part.

[0054] Comparative Example 3

[0055] Comparative Example 3 differs from Example 1 in that the composition of Comparative Example 3 only adds one plant active ingredient, and the composition includes the following components in mass parts: deionized water 91 parts, pea polypeptide 8 parts, 1,2-hexanediol 1 part.

[0056] Comparative Example 4

[0057] Comparative Example 4 differs from Example 1 in that the composition of Comparative Example 4 only adds one plant active ingredient, and the composition includes the following components in mass parts: deionized water 91.5 parts, red bean polypeptide 7.5 parts, 1,2-hexanediol 1 part.

[0058] Comparative Example 5

[0059] Comparative Example 5 differs from Example 1 in that the composition of Comparative Example 5 only adds one plant active ingredient, and the composition includes the following components in mass parts: deionized water 93.5 parts, kava leaf / root / stem extract 5.5 parts, 1,2-hexanediol 1 part.

[0060] Comparative Example 6

[0061] Comparative Example 6 differs from Example 1 in that the composition of Comparative Example 6 only adds one plant active ingredient, and the composition includes the following components in mass parts: deionized water 86.5 parts, grapevine extract 12.5 parts, 1,2-hexanediol 1 part.

[0062] Comparative Example 7

[0063] Comparative Example 7 differs from Example 1 in that the mung bean polypeptide in the composition of Comparative Example 7 is replaced by soybean polypeptide, and the composition includes the following components in mass parts: deionized water 57 parts, soybean polypeptide 8.5 parts, pea polypeptide 8 parts, red bean polypeptide 7.5 parts, kava leaf / root / stem extract 5.5 parts, grapevine extract 12.5 parts, 1,2-hexanediol 1 part.

[0064] Comparative Example 8

[0065] Comparative Example 8 differs from Example 1 in that the pea polypeptide in the composition of Comparative Example 8 is replaced by soybean polypeptide, and the composition comprises the following components in mass fraction: deionized water 57 parts, mung bean polypeptide 8.5 parts, soybean polypeptide 8 parts, adzuki bean polypeptide 7.5 parts, kava leaf / root / stem extract 5.5 parts, grape vine extract 12.5 parts, 1,2-hexanediol 1 part.

[0066] Comparative Example 9

[0067] Comparative Example 9 differs from Example 1 in that the adzuki bean polypeptide in the composition of Comparative Example 9 is replaced by soybean polypeptide, and the composition comprises the following components in mass fraction: deionized water 57 parts, mung bean polypeptide 8.5 parts, pea polypeptide 8 parts, soybean polypeptide 7.5 parts, kava leaf / root / stem extract 5.5 parts, grape vine extract 12.5 parts, 1,2-hexanediol 1 part.

[0068] Comparative Example 10

[0069] Comparative Example 10 differs from Example 1 in that the kava leaf / root / stem extract in the composition of Comparative Example 10 is replaced by gynura extract, and the composition comprises the following components in mass fraction: deionized water 57 parts, mung bean polypeptide 8.5 parts, pea polypeptide 8 parts, adzuki bean polypeptide 7.5 parts, gynura extract 5.5 parts, grape vine extract 12.5 parts, 1,2-hexanediol 1 part.

[0070] Comparative Example 11

[0071] Comparative Example 11 differs from Example 1 in that the grape vine extract in the composition of Comparative Example 11 is replaced by grape seed extract, and the composition comprises the following components in mass fraction: deionized water 57 parts, mung bean polypeptide 8.5 parts, pea polypeptide 8 parts, adzuki bean polypeptide 7.5 parts, kava leaf / root / stem extract 5.5 parts, grape seed extract 12.5 parts, 1,2-hexanediol 1 part.

[0072] Application Examples 1-3

[0073] Application Examples 1-3 respectively provide an essence, and the specific formula is shown in Table 2, and the amount is in mass percentage.

[0074] Table 2: Essence formula table of application examples

[0075]

[0076] The preparation method of the essence of Application Examples 1-3 is as follows:

[0077] (1) The B phase raw materials are pre-mixed, heated to 80°C, dissolved uniformly, and used;

[0078] (2) Mix the A phase raw materials, heat to 80°C, and stir well to make them uniformly dispersed; and add the B phase raw materials to the A phase raw materials before homogenization to obtain a mixture AB;

[0079] (3) Mix the C phase raw materials, heat to 70°C, and dissolve them uniformly;

[0080] (4) Homogenize and stir the C phase raw materials in the mixture AB, and then cool after uniform stirring;

[0081] (5) Cool to 45°C, add the D phase raw materials, stir to disperse them uniformly, and vacuum degas.

[0082] Application Comparative Examples 1-12

[0083] Application Comparative Examples 1-12 each provide a serum. Compared with Application Example 1, the serum of Application Comparative Example 1-11 has the composition of Example 1 replaced by the composition of Comparative Example 1-11, respectively; and Application Comparative Example 12 does not add the composition of Example 1, serving as a blank control.

[0084] The preparation method of the serum of Application Comparative Examples 1-12 is the same as that of Application Example 1, and will not be repeated here.

[0085] Product efficacy test

[0086] Test Example 1 DPPH free radical scavenging rate test

[0087] Test object: the plant polypeptide-containing compositions of Examples 1-3 and Comparative Examples 1-11 of the application.

[0088] Test method and result calculation: the compositions of Examples 1-3 and Comparative Examples 1-11 were prepared into sample liquids of 600 mg / mL in advance for standby. 1 mL of the sample liquid of each of Examples 1-3 and Comparative Examples 1-11 was mixed with 10 mL of a DPPH-ethanol solution (0.05 mg / mL) uniformly, and was placed in the dark at room temperature for 40 min. After the reaction was completed, the reaction liquid was placed in a centrifuge and centrifuged at 3500 rpm for 10 min, and the supernatant was measured for absorbance (A0) at a wavelength of 519 nm. 1 mL of anhydrous ethanol and deionized water were taken to perform the same operation to measure absorbance A1 and A2. 1 mL of a vitamin C solution (1 mg / mL) was taken as a positive control, and each test sample was made in triplicate. The free radical scavenging rate of the plant polypeptide-containing composition of each group was calculated according to formula (1), and the average value was taken as the result.

[0089] DPPH scavenging rate = [1-(A0-A1) / A2] x 100% formula (1).

[0090] The test results are shown in Table 3 below:

[0091] Table 3DPPH radical scavenging rate test results

[0092]

[0093]

[0094] As can be seen from the results of Table 3, the compositions of Examples 1-3 have excellent radical scavenging ability, with Example 1 having the most significant radical scavenging effect, with a scavenging rate of 56.81%. Comparative Example 1, which does not contain any component of the composition, is almost unable to achieve the effect of scavenging free radicals. Comparative Examples 2-6 only contain a single component of the composition, and their free radical scavenging effects are not very ideal. Comparative Examples 7-11 replace each component with a raw material component having similar efficacy in turn, and the free radical scavenging effect is improved relative to the composition containing a single component, but is still significantly lower than the free radical scavenging ability of Examples 1-3. It is not difficult to see that a single efficacy component or changing the components in the composition cannot achieve a good free radical scavenging effect. It is shown that the components in the composition provided by the present application have a synergistic effect, and are indispensable. In addition, by studying the ratio of the components in the composition, the composition can be given stronger free radical scavenging ability, and better applied to cosmetics to improve skin problems caused by free radicals.

[0095] Test Example 2Safety test

[0096] Test object: The compositions of Examples 1-3 of the present application and the serums of Application Examples 1-3.

[0097] Test subjects: A total of 60 people, aged 18-35 years old.

[0098] Test method and identification criteria: 0.03 mL of the sample to be tested is placed in the medicine chamber of the patch test tape, and then the patch test tape with the sample is immediately attached to the normal skin of the left forearm of the test subject from the lower part, and the medicine chamber is gently pressed to remove the air and make the test object evenly distributed. At the same time, the test site is marked for observation. After 24 hours, the patch test tape is removed, and the skin condition is observed after half an hour, 24 hours and 48 hours, respectively. The results of the skin reaction are recorded according to the CTFA guidelines for analysis. The blank control group: The same test is performed on the other test arm of the test subject using deionized water.

[0099] The identification criteria for the skin irritation test are shown in Table 4-1 below:

[0100] Table 4-1 Identification criteria for skin irritation test

[0101] Rank Symbol Criteria 0 - Negative reaction: no irritation, no erythema 1 ± Suspect reaction: slight erythema 2 + Weak positive reaction: erythema 3 ++ Strong positive reaction: erythema, papules, vesicles 4 +++ Very strong positive reaction: severe edema, large blisters

[0102] The test results are shown in Table 4-2 below:

[0103] Table 4-2 Skin irritation test results

[0104]

[0105]

[0106] From the results of the patch test, the composition in the present application is relatively mild, safe and non-irritating, and does not cause adverse reactions to the human body. The composition of the present application is applied in the preparation of cosmetics, and the prepared serum is also mild and non-irritating.

[0107] Test Example 3 Human skin moisture content test

[0108] Test object: The serum of the present application application examples 1-3 and application comparative examples 1-12.

[0109] Test subjects: A total of 150 people, aged 18-35 years old.

[0110] Test instrument and method: The skin moisture content Corneometer test probe CM825 (Germany Courage Khazaka Company) was used for testing. After the test subjects washed their faces, they sat still for 30 min under the set humidity and temperature conditions (humidity 50±5%, temperature 26±1℃). Then test on the same position of left and right face respectively, and record the initial moisture content data (D0). After the test, the test subjects were required to use the test sample according to the requirements, once in the morning and once in the evening, for 14 consecutive days, and avoid using other products with similar functions. And on the 7th day and the 14th day, the data D7 and D14 were obtained respectively. All data were measured three times, and the average value was taken.

[0111] The test results are shown in the following table 5:

[0112] Table 5 Moisture content test results

[0113]

[0114]

[0115] From the moisture content test results, it can be seen that the serum of application examples 1-3 has good moisturizing effect. Among them, the moisturizing effect of application example 1 is the most significant, and after 14 days of use, the moisture content of the left and right sides of the face of the subject is increased by 33.99% and 38.89%, respectively. Application comparative examples 1-12 also have a certain moisturizing effect, among which application comparative examples 1 and 12 are obviously poorer due to not containing the composition or the effective components in the composition. The moisturizing effect of application comparative examples 2-11 is also not as good as application examples 1-3. It can be seen that application comparative examples 2-6 only contain a single component in the composition, and application comparative examples 7-11 replace each component with a similar functional component, and the moisturizing effect is not very ideal. It shows that the components in the composition of the application can have a synergistic effect, and can more efficiently improve the problem of dry skin and promote the skin to be more moist and full.

[0116] Test example 4 human skin soothing test

[0117] Test material: serum of application examples 1-3 and application comparative examples 1-12 of the application.

[0118] Test subjects: a total of 150, aged 18-35 years old.

[0119] Test method and evaluation criteria: the inside of the arm of the subject is washed and dried, then 0.05 mL of 0.2% histamine solution is applied to the 2cm x 2cm skin area on the dry inside of the arm, and after the histamine solution is completely absorbed, the test sample is quickly and evenly applied. Start timing after the sample is applied, ask the subject about the itching condition, and observe the skin condition at the same time. Table 6-1 is the soothing efficacy evaluation criteria, and the soothing efficacy test results of the test sample on human skin are shown in Table 6-2.

[0120] Table 6-1 soothing efficacy evaluation criteria

[0121] Evaluation criteria Soothing effect Time to itching relief > 10 min Ineffective Time to itching relief 5-10 min Low effective Time to itching relief 3-5 min Medium effective Time to itching relief < 3 min High effective

[0122] Table 6-2 human skin soothing test results

[0123] Test group Time to itching relief Soothing effect Application Example 1 1 min 01 s High effective Application Example 2 1 min 49 s High effective Application Example 3 2 min 03 s High effective Application Comparative Example 1 12 min 36 s Ineffective Application Comparative Example 2 8 min 06 s Low effective Application Comparative Example 3 9 min 21 s Low effective Application Comparative Example 4 7 min 59 s Low effective Application Comparative Example 5 4 min 31 s Medium effective Application Comparative Example 6 9 min 01 s Low effective Application Comparative Example 7 6 min 30 s Low effective Application Comparative Example 8 7 min 59 s Low effective Application Comparative Example 9 5 min 13 s Low effective Application Comparative Example 10 3 min 53 s Medium effective Application Comparative Example 11 5 min 49 s Low effective Application Comparative Example 12 11 min 19 s Ineffective

[0124] As can be seen from Table 6-2, the serums prepared by applying Examples 1-3 all have high soothing effect, and are obviously superior to all the comparative examples. Among them, the effect of Example 1 is the best, and the skin blistering and redness caused by histamine can be relieved in about 1 min, and the soothing effect on the skin can be achieved quickly. It can also be seen that the serums prepared by deleting or replacing the components of the composition in Comparative Examples 1-12 have a great discount in soothing effect, which further illustrates that the components in the composition of the present application have a synergistic effect, and are indispensable, which is obviously beneficial to improve the soothing effect of the composition.

[0125] Test Example 5 Human Skin Elasticity Test

[0126] Tested substances: serums prepared by applying Examples 1-3 and Comparative Examples 1-12 of the present application.

[0127] Test subjects: a total of 150 people, aged 18-35 years old.

[0128] Test instruments and methods: skin elasticity test probe MPA580 (Germany Courage Khazaka Company) was used for testing. After the test subjects washed their faces, they sat still for 30 min under the set humidity and temperature conditions (humidity 50±5%, temperature 26±1℃). Then the test was carried out on the same position of the left and right faces, and the initial data (D0) was recorded. After the test, the test subjects were required to use the test samples according to the requirements, once in the morning and once in the evening, for 14 consecutive days, and avoid using other products with similar effects. And on the 7th day and the 14th day, the data D7 and D14 were obtained. All data were measured three times, and the average value was taken. The R2 change rate of the composition in different groups was calculated according to formula (2), and the average value was taken.

[0129] R2 change rate = [(D14-D0) / D0]x100% formula (2).

[0130] The test results are shown in Table 7 below:

[0131] Table 7 Test results of elasticity test

[0132]

[0133]

[0134] Note: the closer the R2 value is to 1, the better the skin elasticity per unit time and per unit cross-sectional area.

[0135] As can be seen from Table 7, after the subjects used the test samples for 14 days, the skin elasticity of the subjects was improved to different degrees (R2 values were increased). Among them, the R2 change rate of the application examples 1-3 showed a very obvious increasing trend, while the effect of the comparative examples 1-12 was obviously poorer, so the missing or change of the components in the formula will reduce the tightening effect. Among the application examples 1-3, the R2 change rate of the application example 1 was the largest, which showed that the different proportions of the composition would cause certain differences in the tightening effect. Therefore, the tightening effect of the composition can be maximized by a certain degree of scientific proportioning.

[0136] Test Example 6 Anti-wrinkle test of human skin

[0137] Test substances: The serum of the application examples 1-3 and the comparative examples 1-12 of the application.

[0138] Test subjects: A total of 150 people, aged 28-48 years old, with different degrees of wrinkles around the eyes.

[0139] Test instruments and methods: The subjects were required to use the test samples according to the requirements, once in the morning and once in the evening, for 14 consecutive days, and avoid using other products with similar effects. After 14 days of use, the facial image analyzer VISIA was used to take pictures of the face, and the wrinkle area, wrinkle number and wrinkle depth of the skin were analyzed.

[0140] The VISIA evaluation results are shown in Table 8.

[0141] Table 8 VISIA evaluation results

[0142]

[0143]

[0144] As can be seen from Table 8, after the subjects used the serum containing the composition containing plant polypeptides of the application examples 1-3 for 14 days, the wrinkles around the eyes were obviously improved, the wrinkle area, wrinkle number and wrinkle depth showed a clear downward trend, and the improvement effect was obviously better than that of the comparative examples 1-12. This shows that the composition containing plant polypeptides provided by the application has a synergistic effect between the components, which plays a good role in improving wrinkles and has a clear wrinkle lightening effect. Compared with the application examples 2-3, the effect of the application example 1 is the best, which shows that the same composition components can further optimize the anti-wrinkle effect of the composition by changing the certain proportion.

[0145] Test Example 7 Human trial evaluation test

[0146] Test substances: The serum of the application examples 1-3 and the comparative examples 1-12 of the application.

[0147] Subjects: 150 in total, aged 18-35 years old.

[0148] Test method: Each volunteer used the test serum according to the specified usage method, once in the morning and once in the evening, and avoided using other products with similar effects. After 14 days of use, an objective evaluation was made on the used serum, and a questionnaire was completed. The questionnaire evaluated the test serum from five dimensions of moisturizing effect, soothing effect, firming effect, anti-wrinkle effect and mildness, and set options for different levels of effect, and the volunteers judged according to their actual use experience. All the questionnaires were collected and the results were analyzed.

[0149] The results of the serum human trial evaluation questionnaire are shown in Tables 9-1 and 9-2:

[0150] Table 9-1 Summary of Serum Questionnaire Results

[0151]

[0152]

[0153] Table 9-2 Summary of Serum Questionnaire Results

[0154]

[0155]

[0156] The subjects can objectively reflect their actual feelings of using the product through self-evaluation. As can be seen from the questionnaire results in Tables 9-1 and 9-2, the subjects believe that all the test samples are relatively mild and have no irritation to the skin. At the same time, more than half of the subjects believe that the serum of Examples 1-3 has good moisturizing, soothing, firming and anti-wrinkle effects, among which the evaluation of each dimension of Example 1 is the best, which is deeply recognized by the subjects. At the same time, the subjects believe that the serum of Comparative Examples 1-12 also has certain moisturizing effect, but the soothing, firming and anti-wrinkle effects are obviously not as good as Examples 1-3. This is basically consistent with other test results in the present application, thus fully illustrating that the components of the composition of the present application synergistically play good moisturizing, soothing, firming and anti-wrinkle effects, which are indispensable. And the present application can further screen out better composition combinations through scientific proportioning.

[0157] The preferred embodiments of the present application are specifically described above, but the present application is not limited to the described embodiments, and those skilled in the art can make various equivalent modifications or replacements without departing from the spirit of the present application. These equivalent modifications or replacements are all included in the scope defined by the claims of the present application.

Claims

1. A composition characterized in that, The composition comprises, by mass fraction: 1-15 parts of mung bean polypeptide, 1-15 parts of pea polypeptide, 1-15 parts of red bean polypeptide, 2-10 parts of kava leaf / root / stem extract, and 5-20 parts of grape vine extract.

2. The composition of claim 1, wherein, The composition comprises, by mass fraction: 1-12 parts of mung bean polypeptide, 1-12 parts of pea polypeptide, 1-12 parts of red bean polypeptide, 2-9 parts of kava leaf / root / stem extract, and 5-18 parts of grape vine extract.

3. The composition of claim 2, wherein, The composition comprises, by mass fraction: 1-10 parts of mung bean polypeptide, 1-10 parts of pea polypeptide, 1-10 parts of red bean polypeptide, 2-8 parts of kava leaf / root / stem extract, and 5-15 parts of grape vine extract.

4. Process for the preparation of a composition according to any one of claims 1 to 3, characterized in that, The method comprises the following steps: The mung bean polypeptide, the pea polypeptide, the red bean polypeptide, the kava leaf / root / stem extract, and the grape vine extract are mixed to obtain the composition.

5. A cosmetic product, characterized by, The composition comprises the composition according to any one of claims 1-3.

6. The cosmetic product according to claim 5, characterized in that, The added amount of the composition is 1-20 wt%.

7. The cosmetic product according to claim 6, characterized in that, The added amount of the composition is 5-15 wt%.

8. The cosmetic product according to claim 5, characterized in that, The dosage form of the cosmetic is selected from the group consisting of cream, emulsion, water, gel, and mask.

9. A serum, characterized by, The composition comprises the composition according to any one of claims 1-3.

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