A microfluidic fan-shaped biochemical reagent disc assembly and a method for using the same

Abstract

This invention discloses a microfluidic fan-shaped biochemical reagent tray assembly and its usage method, comprising a tray and several reagent trays. The tray has several partitions dividing its interior into several fan-shaped regions. The reagent trays are generally fan-shaped, with their shapes matching the fan-shaped regions. The reagent trays are detachably mounted on the tray. A central hole is provided in the center of the tray, and the inner wall of the central hole has a spherical positioning groove for positioning with a positioning bead on the push rod mechanism of the testing instrument. Each fan-shaped region has a push rod located at the bottom of the tray for lifting a water cup placed in the reagent tray. This invention solves the technical problem of existing reagent tray structures, which are mostly disc-shaped, requiring only one type of sample to be added during testing. This necessitates the use of multiple reagent trays and multiple tests for different testing items to complete all testing procedures, resulting in long testing times.

Description

Technical Field

[0001] This invention relates to the field of blood gas detection technology, specifically to a microfluidic fan-shaped biochemical reagent disk assembly and its usage method. Background Technology

[0002] Sample analyzers are primarily used for determining the biochemical and chemical components in clinical blood or other liquid samples, and are one of the commonly used testing instruments in clinical analysis. Sample analyzers typically include a reagent tray, through which samples are analyzed.

[0003] Utility model application CN202122691369.X discloses a reagent tray and a sample analyzer. The reagent tray of this application includes a sample trough for placing samples. The sample trough comprises a stepped sample filling trough and a liquid storage trough, wherein the depth of the sample filling trough is less than the depth of the liquid storage trough, and a blocking block is provided on the side of the sample filling trough near the liquid storage trough. The blocking block of this application can mark the liquid level in the sample trough, preventing excessive sample from being injected into the sample trough, and can prevent liquid from overflowing from the sample trough when the reagent tray rotates, thus improving the reliability of the reagent tray.

[0004] Patent application number CN201711240619.X discloses a method for coagulation analysis based on a biochemical reagent tray. The method involves adding an excipient to a coagulation reagent stock solution, stirring thoroughly, titrating the coagulation reagent to a concentration of 2.0 μl / drop–5.0 μl / drop, rapidly freezing it under liquid nitrogen, and then freeze-drying it in a lyophilizer to form lyophilized microspheres. These microspheres are then loaded onto a biochemical reagent tray for coagulation analysis. This invention's coagulation analysis method freeze-dries the coagulation reagent into structurally stable, non-collapseable microspheres, which are then loaded into the biochemical reagent tray for testing. The lyophilized excipient used ensures the stability of the microspheres' appearance while achieving analytical results comparable to high-cost analytical instruments without affecting reagent performance.

[0005] The reagent trays in the prior art generally have the following shortcomings:

[0006] First, most of the existing reagent tray structures are disc-shaped, which means that only one type of sample can be added to the reagent tray during testing. For different testing items, multiple reagent trays need to be used and multiple tests need to be performed to complete the entire testing process, resulting in a long testing time.

[0007] Secondly, the number of detection holes in the reagent trays of existing technologies is mostly fixed. However, when the number of detection items is small, using this reagent tray for detection can easily lead to waste of the reagent tray.

[0008] Third, the structure of existing detection instruments is relatively fixed. Improving the structure of the reagent tray requires improving the structure of the detection instrument, resulting in high R&D costs and a long development cycle. Summary of the Invention

[0009] The purpose of this invention is to provide a microfluidic fan-shaped biochemical reagent tray assembly and its usage method, which can solve the technical problems existing in the prior art. Most reagent trays are circular, and only one type of sample can be added to the reagent tray during testing. For different testing items, multiple reagent trays need to be used and multiple tests need to be performed to complete all testing procedures, resulting in long testing time.

[0010] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows:

[0011] A microfluidic fan-shaped biochemical reagent tray assembly includes a tray and several reagent trays. The tray has several partitions that divide the interior of the tray into several fan-shaped regions. The reagent trays are generally fan-shaped, and the shape of the reagent trays matches the fan-shaped regions.

[0012] The reagent tray is detachably mounted on the tray;

[0013] The tray has a central hole in the middle, and the inner wall of the central hole has a spherical positioning groove for positioning with the positioning bead on the push rod mechanism in the tester; each sector area has a push rod at the bottom of the tray for lifting the water cup placed in the reagent tray.

[0014] Preferably, there are 2, 3, 4, or 5 sector-shaped regions.

[0015] The partition has a slot, and the tray has a clearance groove at the corresponding position of the slot on the partition. The slot and the clearance groove are connected. The side of the reagent tray has an elastic arm at the position corresponding to the slot on the partition. The elastic arm has a locking part for engaging with the slot. The reagent tray and the tray are detachably connected through the locking part and the slot.

[0016] Further optimization involves providing a recessed area on the side of the reagent tray, with the elastic arm located within the recess.

[0017] Furthermore, an extension is provided at the edge of the reagent tray, the extension extending beyond the outer diameter of the tray.

[0018] A clearance notch is provided on the side wall of the tray at a position corresponding to the fan-shaped area.

[0019] Preferably, the tray is provided with a first positioning groove corresponding to the fan-shaped area, and the reagent tray is provided with a second positioning groove corresponding to the first positioning groove.

[0020] Each reagent tray has an arc-shaped clearance portion near the center hole that corresponds to the inner diameter of the center hole.

[0021] In addition, this invention also discloses a method for using a microfluidic sector-shaped biochemical reagent disk assembly, the specific steps of which are as follows:

[0022] Step 1: Select the reagent tray according to the testing requirements;

[0023] Step 2: Align the reagent tray with the sector area on the tray, and secure the reagent tray to the tray;

[0024] Step 3: Inject the test sample into the reagent tray according to the test requirements, and place the entire tray into the analyzer for analysis and testing.

[0025] The reagent tray includes a tray base with an overall fan-shaped structure and an adhesive film. The tray base is provided with a sample addition chamber, a buffer chamber, a sample quantification chamber and a mixing chamber connected in sequence by capillary tubes. Near the sample addition chamber, the tray base is provided with a placement chamber and a liquid quantification chamber. The placement chamber is used to place a water cup. The placement chamber is connected to the liquid quantification chamber and the liquid quantification chamber is connected to the mixing chamber.

[0026] An annular flow channel is provided on the disk base near the edge of the disk base, and several detection holes communicating with the annular flow channel are also provided on the disk base.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] This invention sets the reagent tray in a fan-shaped structure and installs it on a tray divided into several fan-shaped areas by partitions. During testing, different test samples can be added to multiple reagent trays in a single reagent tray assembly, enabling simultaneous testing of multiple samples. This not only achieves simultaneous testing of multiple samples but also shortens the testing time, greatly improving the testing efficiency of the samples.

[0029] Meanwhile, in actual use, reagent trays can be selected and assembled according to the number of test items, which can reduce the waste of reagent trays;

[0030] Furthermore, this application uses a tray to load the reagent tray, enabling a detachable connection between the reagent tray and the tray. This eliminates the need to modify the structure of the detector, allowing for the direct use of the microfluidic fan-shaped biochemical reagent tray assembly, reducing the technical modification requirements for the detector, and making implementation easier. Attached Figure Description

[0031] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0032] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0033] Figure 2 This is a schematic diagram of the overall structure of the tray of the present invention.

[0034] Figure 3 For the present invention Figure 2 The main view.

[0035] Figure 4 This is a schematic diagram of the reagent tray of the present invention.

[0036] Figure 5 This is a schematic diagram of the overall structure of the reagent tray base in this invention.

[0037] Figure 6 This is a schematic diagram of the overall structure of the baffle described in this invention.

[0038] Figure 7 For the present invention Figure 6 A magnified view of a portion of point A in the middle.

[0039] Figure 8 This refers to the tray structure described in Embodiment Six of the present invention.

[0040] Figure 9 This is a schematic diagram of the overall structure of the disk-retrieving mechanism in Embodiment 5 of the present invention.

[0041] Figure label:

[0042] 101-Tray, 102-Reagent tray, 103-Divider, 104-Fan-shaped area, 105-Center hole, 106-Top rod, 107-Slot, 108-Relief groove, 109-Elastic arm, 110-Engaging part, 111-Recessed area, 112-First positioning groove, 113-Fixing ring, 114-Arc-shaped relief part, 115-Extension, 116-Relief notch

[0043] 117-Disc base, 118-Adhesive membrane, 119-Sample dispensing chamber, 120-Buffer chamber, 121-Sample quantification chamber, 122-Mixing chamber, 123-Placement chamber, 124-Liquid quantification chamber, 125-Annular flow channel, 126-Piercing needle, 127-First filter column, 128-First waste liquid chamber, 129-Second filter column, 130-Second waste liquid chamber, 131-Vent hole, 132-Straight-through detection port, 133-Vent-type detection port, 134-First vent hole;

[0044] 135-Disc picking mechanism, 136-Outer disc, 137-Support column, 138-Guide column, 139-Limiting plate, 140-Spring, 141-Opening slope, 142-Guide slope, 143-Mounting groove, 144-Slot, 145-Annular notch, 146-Round hole, 147-Sample feeding part, 148-Sample feeding port, 149-Baffle. Detailed Implementation

[0045] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of the embodiments of the invention. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0046] In the description of the embodiments of the present invention, it should be understood that the terms "length", "vertical", "horizontal", "top", "bottom", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of the present invention.

[0047] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of embodiments of the present invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0048] In this embodiment of the invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a communication connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this embodiment of the invention according to the specific circumstances.

[0049] In embodiments of the present invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0050] The following disclosure provides many different implementations or examples for carrying out different structures of the embodiments of the present invention. To simplify the disclosure of the embodiments of the present invention, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the embodiments of the present invention. Furthermore, reference numerals and / or reference letters may be repeated in different examples of the embodiments of the present invention; such repetition is for simplification and clarity and does not in itself indicate a relationship between the various implementations and / or arrangements discussed.

[0051] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0052] Example 1

[0053] See Figures 1-5 This embodiment discloses a microfluidic fan-shaped biochemical reagent tray assembly, including a tray 101 and a plurality of reagent trays 102. The tray 101 is provided with a plurality of partitions 103, which divide the interior of the tray 101 into a plurality of fan-shaped regions 104. The reagent trays 102 are generally fan-shaped, and the shape of the reagent trays 102 matches the fan-shaped regions 104.

[0054] The reagent tray 102 is detachably mounted on the tray 101;

[0055] The tray 101 has a central hole 105 in the middle, and the inner wall of the central hole 105 has a spherical positioning groove for positioning with the positioning bead on the push rod mechanism in the tester; each sector area 104 has a push rod 106 located at the bottom of the tray 101 for lifting the water cup placed in the reagent tray 102.

[0056] The present invention sets the reagent tray 102 into a fan-shaped structure and installs the fan-shaped reagent tray 102 on a tray 101 divided into several fan-shaped areas 104 by a partition 103. During testing, different test samples can be added to multiple reagent trays 102 in a reagent tray 102 assembly, realizing the simultaneous detection of multiple samples, which can shorten the detection time and greatly improve the detection efficiency of the samples.

[0057] Meanwhile, in actual use, the reagent tray 102 can be selected and assembled according to the number of test items, which can reduce the waste of the reagent tray 102. Furthermore, this application uses the tray 101 to load the reagent tray 102, realizing the detachable connection between the reagent tray 102 and the tray 101. Without modifying the structure of the detector, the microfluidic fan-shaped biochemical reagent tray assembly can be used directly, reducing the technical modification requirements of the detector and making it easier to implement.

[0058] The fan-shaped regions 104 can be 2, 3, 4 or 5. In this embodiment, there are 3 fan-shaped regions 104. In this way, in actual use, it is possible to detect 3 different samples, which improves the detection efficiency.

[0059] The partition 103 is provided with a slot 107, and the tray 101 is provided with a relief groove 108 at the corresponding position of the slot 107 on the partition 103. The slot 107 and the relief groove 108 are connected. The side of the reagent tray 102 is provided with an elastic arm 109 at the position corresponding to the slot 107 on the partition 103. The elastic arm 109 is provided with a locking part 110 for locking and engaging with the slot 107. The reagent tray 102 and the tray 101 are detachably connected through the locking part 110 and the slot 107.

[0060] The locking part 110 provided on the elastic arm 109 is used to cooperate with the slot 107, thereby fixing the reagent tray 102 and the tray 101.

[0061] The reagent tray 102 has a recessed area 111 on its side, and the elastic arm 109 is located in the recessed area. The recessed area 111 can form a clearance area, which facilitates the clearance when the reagent tray 102 is installed on the tray 101 for fastening.

[0062] The tray 101 is provided with a first positioning groove 112 corresponding to the sector area 104, and the reagent tray 102 is provided with a second positioning groove corresponding to the first positioning groove 112. The first positioning groove 112 and the second positioning groove facilitate the positioning of the tray 101 and the reagent tray 102.

[0063] Among them, the reagent tray 102 is provided with an arc-shaped relief part 114 corresponding to the inner diameter of the central hole 105 near the central hole 105.

[0064] Example 2

[0065] See Figures 1-4 This embodiment is a further optimization based on the first embodiment. In this embodiment, an extension 115 is provided at the edge of the reagent tray 102. The extension 115 extends beyond the outer diameter of the tray 101. The extension 115 makes it more convenient to remove the reagent tray 102 from the tray 101.

[0066] Further optimization involves providing a clearance notch 116 on the side wall of tray 101 at a position corresponding to the fan-shaped area 104. In actual use, when removing reagent tray 102 from tray 101, reagent tray 102 is removed through the clearance notch 116.

[0067] Example 3

[0068] This embodiment discloses a method for using a microfluidic sector-shaped biochemical reagent disk assembly, including the use of the microfluidic sector-shaped biochemical reagent disk assembly described in Embodiment 1. The specific usage steps are as follows:

[0069] Step 1: Select reagent tray 102 according to the testing requirements;

[0070] Step 2: Align the reagent tray 102 with the sector area 104 on the tray 101, and fix the reagent tray 102 on the tray 101;

[0071] Step 3: According to the testing requirements, inject the test sample into the reagent tray 102, and place the entire tray 101 into the testing instrument for analysis and testing.

[0072] Example 4

[0073] See Figures 4-7This embodiment discloses a microfluidic fan-shaped biochemical reagent tray. The reagent tray 102 includes a tray base 117 with an overall fan-shaped structure and an adhesive film 118. The tray base 117 is provided with a sample dispensing chamber 119, a buffer chamber 120, a sample quantification chamber 121, and a mixing chamber 122 connected in sequence by capillaries. Near the sample dispensing chamber 119, the tray base 117 is provided with a placement chamber 123 and a liquid quantification chamber 124. The placement chamber 123 is used to place a water cup and is connected to the liquid quantification chamber 124. The liquid quantification chamber 124 is connected to the mixing chamber 122. The adhesive film 118 is used to adhere to the tray base 117, so that each open cavity or channel of the tray base 117 forms a closed structure.

[0074] An annular flow channel 125 is provided on the disk base 117 near the edge of the disk base 117, and a number of detection holes communicating with the annular flow channel 125 are also provided on the disk base 117.

[0075] In actual use, the bottom of the placement cavity 123 on the tray 117 is made of elastic material. A baffle 149 is provided above the water cup in the placement cavity 123. A piercing needle 126 is provided on the baffle 149. The piercing needle 126 has a V-shaped structure. A sample feeding part 147 is also provided on the baffle 149. The sample feeding part 147 has a funnel-shaped structure. A sample feeding port 148 corresponding to the sample feeding part 147 is provided on the adhesive film 118. In actual use, when the reagent tray 102 is installed on the tray 101, the water cup in the placement chamber 123 moves toward the piercing needle 126 under the action of the push rod 106, and the piercing needle 126 pierces the water cup. The sample is added to the sample addition chamber 119 from the sample addition port 148 by the pipette. When the tester drives the tray 101 to rotate, the sample enters the sample quantitative chamber 121 under the action of centrifugal force, while the liquid in the water cup enters the liquid quantitative chamber 124. When the tray 101 rotates again, the sample in the sample quantitative chamber 121 and the liquid in the liquid quantitative chamber 124 will enter the mixing chamber 122 for mixing.

[0076] As an alternative, in actual use, the tray 117 is provided with a first through hole and a guide surface is provided on the first through hole. An elastic membrane is provided inside the placement cavity 123. When the water cup is placed on the elastic membrane, the reagent tray 102 is installed on the tray 101 so that the push rod 106 passes through the first through hole and squeezes the elastic membrane, thereby causing the water cup to move toward the puncture needle 126.

[0077] Finally, centrifugation is used to make the mixed liquid flow through the annular flow channel 125 and then enter the detection holes in sequence to achieve the purpose of detection.

[0078] Further optimization involves installing several first filter columns 127 in the channel between the sample addition chamber 119 and the buffer chamber 120 in actual use. The sample quantification chamber 121 is also connected to a first waste liquid chamber 128. A second filter column 129 is installed in the channel between the sample quantification chamber 121 and the first waste liquid chamber 128. The sample solution in the sample quantification chamber 121 enters the mixing chamber 122 after passing through the second filter column 129.

[0079] The first filter column 127 and the second filter column 129 can filter out linear impurities in the sample, reducing the chance of capillary blockage in the reagent tray 102 and improving the accuracy of the test results.

[0080] The sample quantitative chamber 121, the mixing chamber 122, the liquid quantitative chamber 124, and the end of the annular flow channel 125 are all provided with a second waste liquid chamber 130 and an exhaust port 131.

[0081] In practical use, the detection orifice includes a straight-through detection orifice 132 and a venting detection orifice 133. The straight-through detection orifice 132 is directly connected to the annular flow channel 125 through a flow channel. The venting detection orifice 133 is connected to the annular flow channel 125 through a capillary tube. The venting detection orifice 133 is also connected to a first venting orifice 134. A molecular plug is provided inside the first venting orifice 134. The molecular plug is used for air permeability and to block liquid.

[0082] When using this device, for tests with lower contamination requirements, the straight-through detection port 132 can be used directly. Liquid inlet and venting are performed simultaneously. Gas is vented into the annular flow channel 125. When liquid enters the straight-through detection port 132, the lyophilized beads in the straight-through detection port 132 will dissolve. During venting, the melted lyophilized reagent can easily be carried out, causing sample contamination. Therefore, for tests with higher contamination requirements, the venting detection port 133 can be used. After the solution enters the venting detection port 133, the air inside is squeezed out from the first venting port 134, preventing the lyophilized reagent from entering the annular channel.

[0083] Example 5

[0084] See Figure 8 This embodiment is a further optimization based on Embodiment 2. In actual use, it was found that when the reagent tray 102 is removed from the clearance notch 116, the reagent tray 102 and the partition 103 are easily damaged because the engaging part 110 and the slot 107 are connected by engaging. Therefore, in actual use, the microfluidic fan-shaped biochemical reagent tray assembly also includes a tray removal mechanism 135, which is used to remove the reagent tray 102 from the tray 101.

[0085] The tray-retrieving mechanism 135 includes an outer plate 136, a support column 137 disposed within the outer plate 136, a guide column 138 disposed in the middle of the outer plate 136, a spring 140 fitted on the guide column 138, and a limiting plate 139 slidably disposed on the guide column 138. The upper end of the spring 140 is connected to the limiting plate 139. The inner diameter of the outer plate 136 is the same as the inner diameter of the tray 101. The position of the support column 137 corresponds to the clearance groove 108 provided on the tray 101. The support column 137 is provided with an opening inclined surface 141. The engaging part 110 is provided with a guide inclined surface 142. The limiting plate 139 is mounted on the guide column 138 through a sliding sleeve.

[0086] After removing the reagent tray 102 from the testing instrument, install the center hole 105 of the reagent tray 102 onto the guide post 138. At this time, press the reagent tray 102 down slightly so that the bottom of the tray 101 contacts the support post 137. Rotate the tray 101 so that the clearance groove 108 is aligned with the support post 137. Then press the tray 101, and the spreading inclined surface 141 on the support post 137 will contact the guide inclined surface 142 on the locking part 110, causing the elastic arm 109 to move toward the recessed area 111. This releases the locking state between the reagent tray 102 and the tray 101, preventing damage to the reagent tray 102. At the same time, all reagent trays 102 can be removed at once, improving tray removal efficiency.

[0087] Example 6

[0088] See Figure 9 This embodiment is basically the same as Embodiment 1. The difference is that, in actual use, different specifications of reagent tray 102 structures can be set according to the needs of detection, specifically: fan-shaped reagent tray 102 structures with 4 detection holes, 8 detection holes, 12 detection holes, 18 detection holes or 32 detection holes, so that there are more options when performing detection.

[0089] In practice, the partition 103 and the tray 101 are connected in a detachable manner, so that the partition 103 can be installed as needed. By adding or removing the partition 103, the tray 101 can be divided into sector areas 104 with the same or different areas, so as to realize the installation of reagent trays 102 of different specifications.

[0090] In specific implementation, a number of mounting grooves 143 are provided on the bottom of the tray 101, and a number of slots 144 corresponding to the mounting grooves 143 are provided on the side wall of the tray 101. The slots 144 are connected to the mounting grooves 143. The mounting grooves 143 are T-shaped grooves or dovetail grooves. A mating part corresponding to the mounting grooves 143 is provided below the partition 103. The partition 103 is mated with the mounting grooves 143 through the mating part. An annular notch 145 is provided on the outer side of the tray 101. A fixing ring 113 for fixing the partition 103 is installed in the annular notch 145.

[0091] The partition 103 is firmly fixed to the tray 101 by the retaining ring 113.

[0092] Of course, in actual use, the tray 101 is provided with a circular hole 146, and the push rod 106 is installed in the circular hole 146 by interference fit. This allows the position of the push rod 106 to be adjusted according to the model of the reagent tray 102. The reagent tray 102 can be freely assembled according to testing requirements to accommodate different specifications of reagent tray 102 structures. It should be noted that the circular hole 146 on the tray 101 is used to install the push rod 106. Therefore, in actual use, the position of the circular hole 146 needs to be determined according to the pre-installed assembly of the reagent tray 102, which will not be elaborated further here.

[0093] In actual use, each mounting slot 143 corresponds to a clearance slot 108.

[0094] This application fixes the partition 103 by sliding it onto the tray 101 and then using a retaining ring 113 to install it onto the tray 101.

[0095] Although preferred embodiments of the invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including both the preferred embodiments and all changes and modifications falling within the scope of the invention.

[0096] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. It should be noted that any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A microfluidic sector-shaped biochemical reagent disk assembly, characterized in that: It includes a tray and several reagent trays. The tray is provided with several partitions, which divide the interior of the tray into several fan-shaped areas. The reagent trays are in a fan-shaped structure, and the shape of the reagent trays matches the fan-shaped areas. The reagent tray is detachably mounted on the tray; The tray has a central hole in the middle, and the inner wall of the central hole has a spherical positioning groove for positioning with the positioning bead on the push rod mechanism in the tester; each sector area has a push rod at the bottom of the tray for lifting the water cup placed in the reagent tray. The partition is provided with a slot, and the tray is provided with a clearance slot at the corresponding position of the slot on the partition. The slot and the clearance slot are connected. The side of the reagent tray is provided with an elastic arm at the position corresponding to the slot on the partition. The elastic arm is provided with a locking part for engaging with the slot. The reagent tray and the tray are detachably connected through the locking part and the slot. The microfluidic sector-shaped biochemical reagent tray assembly also includes a tray removal mechanism for removing the reagent tray from the tray; The tray-retrieving mechanism includes an outer plate, a support column inside the outer plate, a guide column located in the middle of the outer plate, a spring fitted on the guide column, and a limiting plate slidably mounted on the guide column. The upper end of the spring is connected to the limiting plate. The inner diameter of the outer plate is the same as the inner diameter of the tray. The position of the support column corresponds to the clearance groove on the tray. The support column is provided with an opening ramp, and the engaging part is provided with a guide ramp. The limiting plate is mounted on the guide column through a sliding sleeve.

2. The microfluidic sector-shaped biochemical reagent disk assembly according to claim 1, characterized in that: The reagent tray has a recessed area on its side, and the elastic arm is located within the recess.

3. The microfluidic sector-shaped biochemical reagent disk assembly according to claim 1, characterized in that: An extension is provided at the edge of the reagent tray, and the extension extends beyond the outer diameter of the tray.

4. A microfluidic sector-shaped biochemical reagent disk assembly according to claim 3, characterized in that: A clearance notch is provided on the side wall of the tray at a position corresponding to the fan-shaped area.

5. A microfluidic sector-shaped biochemical reagent disk assembly according to any one of claims 1-4, characterized in that: The tray is provided with a first positioning groove corresponding to the fan-shaped area, and the reagent tray is provided with a second positioning groove corresponding to the first positioning groove.

6. A microfluidic sector-shaped biochemical reagent disk assembly according to claim 5, characterized in that: The reagent tray has an arc-shaped clearance section near the center hole that corresponds to the inner diameter of the center hole.

7. A microfluidic sector-shaped biochemical reagent disk assembly according to any one of claims 1-4, characterized in that: The reagent tray includes a tray base with an overall fan-shaped structure and an adhesive film. The tray base is provided with a sample dispensing chamber, a buffer chamber, a sample quantification chamber and a mixing chamber connected in sequence by capillary tubes. Near the sample dispensing chamber, the tray base is provided with a placement chamber and a liquid quantification chamber. The placement chamber is used to place a water cup. The placement chamber is connected to the liquid quantification chamber and the liquid quantification chamber is connected to the mixing chamber. An annular flow channel is provided on the disk base near the edge of the disk base, and several detection holes communicating with the annular flow channel are also provided on the disk base.

8. A method of using a microfluidic sector-shaped biochemical reagent disk assembly, characterized in that, The microfluidic sector-shaped biochemical reagent disk assembly according to any one of claims 1-7 is used, and the specific steps are as follows: Step 1: Select the reagent tray according to the testing requirements; Step 2: Align the reagent tray with the sector area on the tray, and secure the reagent tray to the tray; Step 3: Inject the test sample into the reagent tray according to the test requirements, and place the entire tray into the analyzer for analysis and testing.

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