A device for testing the motion behavior of daphnia magna

Abstract

The present application relates to a kind of large daphnia motion behavior testing device, it includes box, box has openable door body, driving assembly is positioned in box and is driven carousel rotation, and the periphery of carousel upper surface is equipped with multiple culture bottle that can be taken and placed, box inside one side is fixed with each other and is closely adjacent to pre-processing chamber and test chamber, pre-processing chamber is surrounded by light shield, and one side wall is equipped with import, the side wall of test chamber is equipped with export, pre-processing chamber and test chamber are equipped with communicating port, when carousel rotates, each culture bottle will be in turn through import, communicating port and export, pre-processing chamber is equipped with sensor assembly, test chamber is fixed with variable light source and camera, driving assembly, sensor assembly, variable light source and camera are electrically connected with controller.The advantages are integrated design, avoid the disturbance brought by conversion between parallel experiments;High degree of integration and high throughput, once setting can complete multiple experimental tests, greatly improve test efficiency.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of daphnia magna motion behavior test, and particularly relates to a daphnia magna motion behavior test device. BACKGROUND

[0002] With the intensification of human activities, more and more chemicals are synthesized and used, and then enter the environment medium, causing potential threats to the ecosystem and human health. Implementing pollutant monitoring and understanding the multi-dimensional characteristics of chemical pollutants in water bodies is one of the important links in response to chemical pollution. Daphnia magna is the most widely distributed and most widely studied representative species of plankton in freshwater ecosystems, so using this species to study the multi-dimensional toxicity of pollutants and the water ecological suitability has the characteristics of uniformity of experimental methods and universality of experimental results.

[0003] Daphnia magna motion behavior studies the swimming time, speed, acceleration, activity, jumping frequency, swimming distance / trajectory, turning frequency / angle, resting time, sinking rate and motion behavior in the vertical direction of Daphnia magna. Since the distribution and motion behavior of Daphnia magna in the water body are usually closely related to the hydrological physical conditions it adapts to, such as light, temperature, salinity, water flow, etc. Therefore, the ecological environment of the water body can be monitored and evaluated according to the research results of the motion behavior of Daphnia magna. In the past, the study of the influence of pollutants on the life history characteristics of Daphnia magna is one of the most common and most reliable evaluation methods. However, in recent years, the study of the influence of pollutants on the behavior of Daphnia magna has attracted more and more attention, on the one hand because the life history characteristics research observation index is more, the process is longer; on the other hand because the behavior response trigger threshold is lower, the response is more rapid. Therefore, the study of the influence of pollutants on the behavior of Daphnia magna is more suitable for rapid identification (low and high concentrations are applicable). The current developed Daphnia magna behavior research index system includes: swimming time, speed, acceleration, activity, jumping frequency, swimming distance / trajectory, turning frequency / angle, resting time, sinking rate, etc. The corresponding research means is mainly image recording and analysis, and the recording and / or analysis software and hardware that have been applied to specific practice include and , etc.

[0004] is a commonly used video analysis software for studying the motion behavior of Daphnia magna. Using software to study the motion behavior of Daphnia magna needs to go through six steps of data acquisition, video import, marking Daphnia magna, tracking motion, motion trajectory analysis, and data export and analysis. The data acquisition first needs to record the motion trajectory of Daphnia magna through a camera device (such as an underwater camera) to form video materials. Then import the collected video into software, mark Daphnia magna through the marking tool in software, and then The software tracks the movement of Daphnia magna and records, The trajectory of Daphnia magna is generated. Finally, the software's measurement tool is used to analyze the movement speed, direction, and other related parameters of Daphnia magna. The disadvantages are: the process of data collection and recording the trajectory of Daphnia magna is relatively complex, and the quality of the collected video is required to be high.

[0005] DaphniaToximeter is a high-sensitivity toxicity detection device that uses Daphnia magna as a probe to detect the impact of water samples on the number, movement speed, swimming height, and tour frequency of Daphnia magna. It uses camera and graph analysis technology to continuously detect the impact of the measured sample on the activity of Daphnia magna, and then determines the strength of the water sample toxicity. The device exposes Daphnia magna samples to the test substance, observes and records the response and behavior changes of Daphnia magna. This includes activity, feeding ability, growth and development, reproductive capacity, and other indicators. By observing and analyzing these indicators, the toxicity level of the test substance can be evaluated. By using DaphniaToximeter, potential toxic substances in the environment, such as industrial wastewater, pesticides, heavy metals, etc., can be detected, thereby evaluating the environmental quality of water bodies and the health of the ecosystem. The disadvantages are: mainly used to monitor the response and behavior of Daphnia magna under the exposure of toxic substances, to analyze and judge the strength of water sample toxicity, but relatively weak in the imaging of Daphnia magna trajectory, unable to directly display the movement trajectory of Daphnia magna (especially the vertical trajectory), and the device structure is relatively complex, the operation difficulty is large, and high-throughput, integrated testing cannot be realized. SUMMARY

[0006] The present application provides a Daphnia magna movement behavior testing device, which aims to overcome the above-mentioned problems in the prior art.

[0007] The technical scheme for solving the above technical problems of the present application is as follows: a large Daphnia motion behavior testing device, comprising a box body, the box body is provided with an openable door body, a rotating disc and a driving assembly for driving the rotating disc to rotate and position are arranged in the box body, a plurality of culture bottles capable of being taken and placed are arranged on the periphery of the upper surface of the rotating disc in a circumferential direction, a pretreatment chamber and a testing chamber are fixedly arranged on one side of the box body and are adjacent to each other, the pretreatment chamber is surrounded by a light shielding plate and is provided with an inlet on one side wall, the testing chamber is provided with an outlet on one side wall, a communication port for communicating the pretreatment chamber and the testing chamber is arranged on the light shielding plate between the pretreatment chamber and the testing chamber, when the rotating disc rotates, each culture bottle will pass through the inlet, the communication port and the outlet in turn, the pretreatment chamber is provided with a sensor assembly for automatically collecting environmental parameters including pH value and dissolved oxygen value of the liquid in the culture bottle, a variable light source and a camera are fixedly arranged in the testing chamber, and the driving assembly, the sensor assembly, the variable light source and the camera are electrically connected with a controller.

[0008] On the basis of the above technical scheme, the present application can also be improved as follows.

[0009] Further, a mounting plate is fixedly arranged in the middle part of the box body in a horizontal direction, the driving assembly comprises a brake motor and a position sensor, the output end of the brake motor is fixedly connected with the center of the rotating disc arranged in a horizontal direction after penetrating through the center hole of the mounting plate in a vertical upward direction, the brake motor is fixedly connected with the mounting plate, the position sensor is fixedly connected with the box body, the rotating disc is arranged in a circumferential direction and is provided with a sensing head matched with the position sensor, when the sensing head triggers the position sensor, the brake motor stops rotating to position the rotating disc.

[0010] Further, the box body comprises an outer frame and an inner support arranged in the outer frame, the outer frame is fixedly provided with a shielding plate constituting the box wall of the box body, and the inner support is arranged between the mounting plate and the bottom wall of the box body to support and fix the mounting plate.

[0011] Further, a temperature control assembly for regulating the temperature in the box body is further arranged in the box body or outside the box body and is in communication with the box body.

[0012] Further, the camera is fixedly connected with the box body through a hanging rod, the camera is arranged above the middle part of the rotating disc and can shoot the culture bottles arranged in the testing chamber, and the variable light source is fixedly arranged on the lower surface of the top wall of the testing chamber and is located between the camera and the culture bottles to be shot.

[0013] Further, the upper surface of the rotating disc is provided with a plurality of placing grooves for placing and limiting the culture bottles, which are uniformly and peripherally spaced, both ends of the groove bottom of each placing groove is provided with two limiting convex columns, both ends of the bottom of the culture bottle is provided with an extension edge, and the extension edge is provided with limiting holes corresponding to the limiting convex columns.

[0014] Further, self-resetting light-shielding strips are arranged at the inlet and the communication port, when the culture bottle rotates with the rotating disc, the self-resetting light-shielding strips can be pressed to open the inlet and the communication port, and after the culture bottle is separated from the self-resetting light-shielding strips, the self-resetting light-shielding strips can automatically close the inlet and the communication port.

[0015] Further, the sensor assembly comprises a pH sensor, a dissolved oxygen sensor and an automatic lifting part, the pH sensor and the dissolved oxygen sensor are driven to be lifted by the automatic lifting part to be inserted downward or to be away upward from the culture bottle in the pretreatment chamber.

[0016] Further, the box body is a cuboid or a square body, the door body is arranged at the top of the box body, the rotating disc is a circular disc, and the area of the pretreatment chamber and the test chamber covering the upper surface of the rotating disc is 1 / 4 to 1 / 3 of the total area of the upper surface of the rotating disc.

[0017] Further, a power supply is arranged in the box body, the driving assembly, the sensor assembly, the variable light source, the camera and the controller are electrically connected with the power supply, the door body comprises two door plates hinged to the box body, a handle is arranged on each door plate, and the two door plates are arranged in an opposite opening mode.

[0018] Compared with the prior art, the device has the following beneficial effects:

[0019] The device avoids disturbance caused by conversion between parallel experiments through integrated structural design, and can effectively avoid external interference; the device has high integration degree, and pretreatment, experimental test and data analysis are highly integrated; the device has the advantages of high throughput, and a plurality of experimental tests can be completed at one time, so that the data collection efficiency is greatly improved; in addition, the device can provide better video imaging and trajectory pictures for capturing the motion trajectory of daphnia magna, and the overall operation of the device is relatively simple. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 The device is provided as an axonometric view of a daphnia magna motion behavior test device;

[0021] Figure 2 The device is provided as an axonometric view of a daphnia magna motion behavior test device; Figure 1 The test device is not shown as an axonometric view of the box wall shielding plate;

[0022] Figure 3 The device is provided as an axonometric view of a daphnia magna motion behavior test device;Figure 2 front view of the test device shown in FIG. 1;

[0023] Figure 4 for Figure 2 top view of the test device shown in FIG. 1;

[0024] Figure 5 for Figure 1 axonometric view of the test device shown in FIG. 1, without the door body, the front tank wall, the right tank wall and the inner and outer frames;

[0025] Figure 6 for Figure 5 top view of the test device shown in FIG. 1;

[0026] Figure 7 for Figure 5 axonometric view of the light shield of the test device shown in FIG. 1;

[0027] Figure 8 for Figure 5 axonometric view of the carousel and the camera, variable light source and other structures above it;

[0028] Figure 9 for Figure 8 rear view of the structure shown in FIG. 1;

[0029] Figure 10 for Figure 8 top view of the structure shown in FIG. 1;

[0030] Figure 11 for Figure 8 enlarged rear view of the structure within dashed circle A.

[0031] In the drawings, the components represented by the respective reference numerals are listed as follows:

[0032] 1, tank; 2, door body; 3, carousel; 4, culture bottle; 5, light shield; 6, pretreatment chamber; 7, test chamber; 8, inlet; 9, outlet; 10, communication port; 11, variable light source; 12, camera; 13, controller; 14, mounting plate; 15, brake motor; 16, position sensor; 17, inductive head; 18, outer frame; 19, inner support; 20, placement groove; 21, limiting protrusion column; 22, outer extension edge; 23, limiting hole; 24, power supply; 25, handle. DETAILED DESCRIPTION

[0033] The principles and features of the present application are described below in conjunction with the drawings, which are provided only for the purpose of explanation and are not intended to limit the scope of the present application.

[0034] In the description of the present application, if the terms of indicating orientation or position relationship such as "upper", "lower", "left", "right", "top", "bottom", "inner", "outer" and the like are used, the indicated orientation or position relationship is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.

[0035] As shown in Figures 1 to 11 The present application provides a large Daphnia behavior testing device, which comprises a box body 1, the box body 1 is provided with an openable and closable door body 2, the box body 1 is internally provided with a rotating disc 3 and a driving assembly for driving the rotating disc 3 to rotate and position, a plurality of culture bottles 4 which can be taken and placed are circumferentially and spaced apart on the upper surface of the rotating disc 3, a pretreatment chamber 6 and a testing chamber 7 which are adjacent to each other are further fixedly arranged on one side in the box body 1, the pretreatment chamber 6 is surrounded by a light shielding plate 5 and is provided with an inlet 8 on one side wall, the testing chamber 7 is provided with an outlet 9 on one side wall, the light shielding plate 5 between the pretreatment chamber 6 and the testing chamber 7 is provided with a communication port 10 which communicates the pretreatment chamber 6 and the testing chamber 7, when the rotating disc 3 rotates, each of the culture bottles 4 will pass through the inlet 8, the communication port 10 and the outlet 9 in turn, the pretreatment chamber 6 is provided with a sensor assembly for automatically collecting environmental parameters including pH value and dissolved oxygen value of the liquid in the culture bottles 4, the testing chamber 7 is fixedly provided with a variable light source 11 and a camera 12, the driving assembly, the sensor assembly, the variable light source 11 and the camera 12 are electrically connected with a controller 13. The variable light source 11 selects LED lamp which can change light color.

[0036] It should be noted that when the door body is opened, each culture bottle (the culture solution and the large water flea are filled in advance, or the culture solution containing corresponding components is prepared according to the experiment or test requirements, or the liquid and the large water flea are directly put into the culture bottle for testing) can be manually and stably placed at the corresponding position on the periphery of the rotating disc, and then the door body is closed, and then the testing device is started, the controller controls the driving assembly, the sensor assembly, the variable light source and the camera to operate according to the set program, each culture bottle rotates and positions with the rotating disc, and each culture bottle will experience the process of entering the pretreatment chamber for dark adaptation and environmental parameter collection (pH value and dissolved oxygen value, etc.) through the inlet, then entering the testing chamber through the communication port for motion behavior observation and recording through the camera, and finally turning out of the testing chamber through the outlet. The test is continuous and progressive, when the large Daphnia in the culture bottle in the testing chamber is observed, the large Daphnia in a plurality of culture bottles in the pretreatment chamber are also simultaneously performing dark adaptation and / or environmental parameter collection.

[0037] It can be understood that the daphnia magna is a type of plankton, and the device provided by the application can be used for observation and testing of other planktons. The daphnia magna can be understood as a representative of the type of plankton in a broad sense.

[0038] In one embodiment of the application, as shown in Figure 2 、 Figure 5 and Figure 8 , a mounting plate 14 is horizontally fixed in the middle of the box 1, the driving assembly includes a brake motor 15 and a position sensor 16, the output end of the brake motor 15 is fixedly connected with the center of the horizontally arranged turntable 3 after vertically penetrating through the center hole of the mounting plate 14, the brake motor 15 is fixedly connected with the mounting plate 14, the position sensor 16 is fixedly connected with the box 1, and the turntable 3 is circumferentially provided with sensing heads 17 matched with the position sensor 16. When the sensing heads 17 trigger the position sensor 16, the brake motor 15 stops transmission to position the turntable 3.

[0039] It should be noted that the position sensor can be a metal proximity switch, and the turntable as a whole or at least the outer edge can be made of a non-metal material. A plurality of metal sensing heads (such as screws) are uniformly and spacedly arranged on the outer edge of the turntable. Each sensing head corresponds to the position of a culture bottle on the turntable. When the turntable rotates, the sensing head is close to and aligned with the position sensor, and the position sensor is triggered instantaneously. The position sensor sends a signal to the pre-controller, and the controller immediately controls the brake motor to stop rotating. Then, the turntable stops rotating, and one of the culture bottles on the turntable is located in the test chamber, and two to three or more culture bottles are located in the pre-treatment chamber (preferably, as shown in Figure 10 , the turntable has a total of 12 culture bottle placing slots, including three pre-treatment stations and one detection station). When the controller receives the position sensor signal, the controller not only controls the motor to stop rotating, but also synchronously starts the camera to observe and shoot. The variable light source is also controlled by the controller to start and stop or adjust the light of different frequencies to irradiate the culture bottles in the test chamber according to the test requirements. When the observation and shooting time of one culture bottle reaches the set time length, the controller controls the brake motor to start again, and the turntable rotates until the next sensing head triggers the position sensor and performs the next round of testing.

[0040] In one embodiment of the application, the box 1 includes an outer frame 18 and an inner support 19 arranged in the outer frame 18. The outer frame 18 is fixed with a shielding plate constituting the wall of the box 1. The inner support 19 is arranged between the mounting plate 14 and the bottom wall of the box 1 to support and fix the mounting plate 14.

[0041] It should be noted that the outer frame and the inner support are preferably made of aluminum profile or aluminum alloy profile, and the shielding plate is preferably made of lightproof plastic plate or thin steel plate, and the shielding plate is fixed to the outer frame by screws or rivets. The inner support not only supports the mounting plate to bear the weight after the motor is installed, but also includes a plurality of support rods extending from the outer frame into the box, some of which are used to support and fix the light shielding plate forming the pretreatment chamber and the test chamber, and some of which are used to fix the camera or the variable light source.

[0042] In an embodiment of the present application, a temperature control assembly for regulating the temperature in the box 1 is further included, which is arranged in the box 1 or outside the box 1 and communicates with the box 1.

[0043] It can be understood that the temperature control assembly is a known structure (such as a commonly used thermostat or air conditioning assembly), which can regulate and maintain the temperature in the box to meet the needs of test personnel to observe and record the movement behavior of Daphnia magna at different temperatures. The temperature control assembly includes a temperature sensor, heating and / or cooling components, the specific structure, installation and control of which are well known to those skilled in the art, and will not be described here.

[0044] In an embodiment of the present application, as shown in Figure 6 , 8 and 9, the camera 12 is fixedly connected to the box 1 by a hanging rod (such as a support rod extending downward from the top of the outer frame), and is located above the middle region of the turntable 3 and can shoot the culture bottle 4 in the test chamber 7. The variable light source 11 is fixed to the lower surface of the top wall of the test chamber 7 and is located between the camera 12 and the culture bottle 4 to be shot.

[0045] It can be understood that in order to more clearly shoot the movement behavior of Daphnia magna, the camera can be equipped with a position adjusting power component connected to the controller, which can be automatically controlled by the controller or manually adjusted by the controller to more clearly and accurately shoot the movement of Daphnia magna.

[0046] In an embodiment of the present application, as shown in Figure 11 , a plurality of placement grooves 20 for placing and limiting the culture bottle 4 are uniformly and spaced apart on the upper surface of the turntable 3. Each of the placement grooves 20 is provided with two limiting protruding columns 21 at both ends of the groove bottom, and the culture bottle 4 has an extended edge 22 at both ends of the bottom, and the extended edge 22 is provided with a limiting hole 23 corresponding to the limiting protruding column 21.

[0047] It should be noted that when the limiting convex is inserted into the limiting hole on the outer extension edge of the two sides of the bottom of the culture bottle (cuvette), the culture bottle will not be displaced when subjected to horizontal force, achieving the purpose of stable placement. When it is necessary to remove the culture bottle, the culture bottle is pulled upward from the door body opened at the top of the box body by artificial, so that the culture bottle is separated from the limiting convex column.

[0048] In an embodiment of the present application, self-resetting light-shielding strips are arranged at the inlet 8 and the communication port 10, and when the culture bottle 4 rotates with the rotating disc 3, the self-resetting light-shielding strips can be pressed to open the inlet 8 and the communication port 10, and after the culture bottle 4 is separated from the self-resetting light-shielding strips, the self-resetting light-shielding strips can automatically close the inlet 8 and the communication port 10.

[0049] It can be understood that in order to ensure a better dark environment in the pretreatment chamber for dark adaptation pretreatment of the daphnia magna in the culture bottle entering the pretreatment chamber, the inlet and the communication port of the pretreatment chamber are preferably also closed and light-shielded when no culture bottle passes through, and therefore the self-resetting light-shielding strips are arranged to achieve the above purpose. When a culture bottle passes through, the self-resetting light-shielding strips are pressed to open the inlet (communication port) or are opened by another power mechanism, and after the culture bottle is separated, the inlet and the communication port are automatically closed.

[0050] In an embodiment of the present application, the sensor assembly includes a pH sensor, a dissolved oxygen sensor, and an automatic lifting part, and the pH sensor and the dissolved oxygen sensor are driven to be lifted downward or away from the culture bottle 4 in the pretreatment chamber 6 by the automatic lifting part.

[0051] It should be noted that the automatic lifting part will only automatically lift to complete the collection of environmental parameters such as pH value and dissolved oxygen value in the culture bottle when the culture bottle is directly below the automatic lifting part. The automatic lifting part can be linked with a position sensor, and when the position sensor is triggered, the automatic lifting part is also triggered and performs an action of first lowering and then collecting data, and then rising to reset according to the set program.

[0052] In an embodiment of the present application, the box body 1 is a rectangular or square body, and the door body 2 is arranged at the top of the box body 1, the rotating disc 3 is a circular disc, and the area of the pretreatment chamber 6 and the test chamber 7 covering the upper surface of the rotating disc 3 is 1 / 4 to 1 / 3 of the total area of the upper surface of the rotating disc 3.

[0053] It should be noted that the above structure design is beneficial to the miniaturization of the entire test device, and can realize parallel testing of multiple culture bottles in the same environment, and also avoids that the device occupies a large space due to too large size.

[0054] In one embodiment of the present application, the box 1 is provided with a power supply 24, and the driving assembly, the sensor assembly, the variable light source 11, the camera 12 and the controller 13 are electrically connected to the power supply 24; the door body 2 comprises two door panels hinged to the box 1, and a handle 25 is arranged on the door panels, and the two door panels are arranged in a double-leaf manner.

[0055] It can be understood that all the electrically driven components of the whole device can be powered by the power supply, and the power supply can be a lithium battery capable of repeated charging and discharging. In addition to the above-mentioned form with a built-in power supply, the test device provided by the present application can also directly use commercial power as the driving power supply.

[0056] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A large-scale daphnia motion behavior testing device, characterized in that, The enclosure includes a housing (1) with an openable and closable door (2). Inside the housing (1) is a turntable (3) and a drive assembly for rotating and positioning the turntable (3). Multiple removable culture bottles (4) are spaced circumferentially around the upper surface of the turntable (3). Inside the housing (1) are fixedly arranged pretreatment chambers (6) and testing chambers (7) adjacent to each other. The pretreatment chamber (6) is enclosed by a light-shielding plate (5) and has an inlet (8) on one side wall. The testing chamber (7) has an outlet (9) on one side wall. A light-shielding plate (5) separates the pretreatment chamber (6) from the testing chamber (7). 5) The pretreatment chamber (6) and the test chamber (7) are connected by a communication port (10). When the turntable (3) rotates, each culture bottle (4) will pass through the inlet (8), the communication port (10) and the outlet (9) in sequence. The pretreatment chamber (6) is equipped with a sensor assembly for automatically collecting environmental parameters, including pH value and dissolved oxygen value, of the liquid in the culture bottle (4). The test chamber (7) is fixedly equipped with a variable light source (11) and a camera (12). The drive assembly, sensor assembly, variable light source (11) and camera (12) are all electrically connected to the controller (13). A mounting plate (14) is horizontally fixed in the middle of the housing (1). The drive assembly includes a brake motor (15) and a position sensor (16). The output end of the brake motor (15) passes vertically upward through the center hole of the mounting plate (14) and is fixedly connected to the center of the horizontally set turntable (3). The brake motor (15) is fixedly connected to the mounting plate (14), and the position sensor (16) is fixedly connected to the housing (1). The turntable (3) is provided with sensing heads (17) that cooperate with the position sensor (16) at intervals in the circumferential direction. When the sensing head (17) triggers the position sensor (16), the brake motor (15) stops rotating, so that the turntable (3) is positioned.

2. The large-scale daphnia motion behavior testing device according to claim 1, characterized in that, The box (1) includes an outer frame (18) and an inner support (19) disposed within the outer frame (18). A baffle plate constituting the box wall of the box (1) is fixed on the outer frame (18). The inner support (19) is disposed between the mounting plate (14) and the bottom wall of the box (1) to support and fix the mounting plate (14).

3. The large-scale daphnia motion behavior testing device according to claim 1, characterized in that, It also includes a temperature control component for regulating the temperature inside the box (1), the temperature control component being located inside the box (1) or outside the box (1) and communicating with the inside of the box (1).

4. The large-scale daphnia motion behavior testing device according to claim 1, characterized in that, The camera (12) is fixedly connected to the box (1) by a hanging rod. The camera (12) is located above the middle area of ​​the turntable (3) and can take pictures of the culture bottle (4) located in the test chamber (7). The variable light source (11) is fixed to the lower surface of the top wall of the test chamber (7) and is located between the camera (12) and the culture bottle (4) to be photographed.

5. A large-scale daphnia motion behavior testing device according to claim 1, characterized in that, The upper surface of the turntable (3) is provided with a plurality of placement slots (20) evenly spaced around the periphery for placing and limiting the culture bottle (4). Each placement slot (20) has two limiting protrusions (21) at both ends of the bottom. The bottom ends of the culture bottle (4) have an outer edge (22) and a limiting hole (23) corresponding to the limiting protrusion (21) on the outer edge (22).

6. A large-scale daphnia motion behavior testing device according to claim 1, characterized in that, Both the inlet (8) and the connecting port (10) are provided with self-resetting light-shielding strips. When the culture bottle (4) rotates with the turntable (3), it can squeeze the self-resetting light-shielding strips to open the inlet (8) and the connecting port (10). After the culture bottle (4) is removed from the self-resetting light-shielding strips, the self-resetting light-shielding strips can automatically close the inlet (8) and the connecting port (10).

7. A large-scale daphnia motion behavior testing device according to claim 1, characterized in that, The sensor assembly includes a pH sensor, a dissolved oxygen sensor, and an automatic lifting unit. The pH sensor and the dissolved oxygen sensor are both driven to move up and down by the automatic lifting unit to insert downwards into or move upwards away from the culture flask (4) in the pretreatment chamber (6).

8. A large-scale daphnia motion behavior testing device according to claim 1, characterized in that, The box (1) is rectangular or cubic and the door (2) is located on the top of the box (1). The turntable (3) is a circular disc. The pretreatment chamber (6) and the test chamber (7) cover the upper surface of the turntable (3) with an area of ​​1 / 4 to 1 / 3 of the total area of ​​the upper surface of the turntable (3).

9. A large-scale daphnia motion behavior testing device according to any one of claims 1 to 8, characterized in that, The housing (1) is equipped with a power supply (24). The drive assembly, sensor assembly, variable light source (11), camera (12) and controller (13) are all electrically connected to the power supply (24). The door (2) includes two door panels that are hinged to the housing (1). The door panels are equipped with handles (25). The two door panels are designed to open in opposite directions.

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