A western blotting immobilization clamp expander

Abstract

The present application relates to experimental equipment technical field, specifically to a kind of western blotting fixing clamp dilator, it includes: electrophoresis tank, the two sides of electrophoresis tank structure cavity are equipped with expansion mechanism and expansion support respectively, and structure cavity top is equipped with the pressure rod of trigger expansion mechanism and electrode, and electrophoresis circuit is equipped between two electrodes;Double glass plate, double glass plate is made of two overlapping glass slides, and U-shaped silica gel pad is equipped between two glass slides;Clamping cover, clamping cover is set in pairs, respectively hinged in the two ends of electrophoresis tank, for constraining double glass plate between expansion support and clamping cover;Container, container is used to carry electrophoresis tank, and the inner wall of opposite two sides of container is respectively equipped with transfer mold circuit, and each transfer mold circuit is respectively equipped with joint extending to outside through the side wall of container.The present application is improved to electrophoresis tank, the operation equipment and experimental procedure of traditional WB experiment are simplified, and the problem of glass plate sealing is avoided in electrophoresis step.

Description

Technical Field

[0001] This invention relates to the field of experimental equipment technology, specifically to a protein blot fixation clip expander. Background Technology

[0002] Electrophoresis tanks are devices used in biochemical research to identify the components of proteins, separating mixed protein samples into individual components under an electric field. Traditional Western blotting (WB) experiments require a separate gel preparation device, and after gel preparation, the glass plates must be removed from the preparation device and installed onto the traditional electrophoresis tank. A mechanism in the traditional electrophoresis tank is then pressed to achieve a seal during electrophoresis. Therefore, traditional WB experiments are cumbersome, involve numerous devices, and cause inconvenience. Furthermore, the clamping force of existing electrolytic cell flippers is limited, often failing to provide reliable clamping force for the double glass plates, thus easily leading to sealing instability problems in traditional WB experiments. Summary of the Invention

[0003] This invention provides a protein blot fixation clip expander, which simplifies the WB experimental steps and apparatus by improving the electrophoresis tank, and avoids the problem of poor sealing of the glass plate in the electrophoresis step.

[0004] To achieve the above objectives, the present invention provides the following technical solution: a protein blot fixation clip expander, comprising: an electrophoresis tank, wherein structural cavities are respectively provided at both ends of the electrophoresis tank, and expansion mechanisms are respectively provided on both sides of the electrophoresis tank for each structural cavity; a pressure rod for triggering the expansion mechanism and an electrode are provided at the top of each structural cavity; an electrophoresis circuit is provided between two electrodes; an expansion support is disposed on both sides of the electrophoresis tank and connected to the corresponding expansion mechanism; a silicone strip is respectively provided on the outer side of each expansion support; a double glass plate is composed of two overlapping glass slides, and a U-shaped silicone pad is provided between the two glass slides; a clamping cover is provided in pairs and respectively hinged to both ends of the electrophoresis tank for constraining the double glass plate between the expansion support and the clamping cover; a container is used to carry the electrophoresis tank, and a mold transfer circuit is respectively provided on the inner walls of opposite sides of the container; each mold transfer circuit is provided with a connector extending outward through the side wall of the container.

[0005] Preferably, the expansion mechanism includes a push rod corresponding to the expansion bracket and a gear transmission mechanism disposed between the push rod and the pressure rod. The gear transmission mechanism includes a first rack disposed along the pressure rod, a first gear meshing with the first rack, a first bevel gear coaxially disposed on both sides of the first gear, a second bevel gear meshing with the first bevel gear, a second gear coaxially disposed at the bottom of the second bevel gear, and a second rack disposed along the push rod, wherein the second gear meshes with the second rack. The axis of the first gear is parallel to the length direction of the push rod, and the axis of the second gear is parallel to the length direction of the pressure rod.

[0006] Preferably, the expansion mechanism further includes a locking mechanism disposed within the structural cavity and cooperating with the bottom end of the pressure rod. The locking mechanism includes a barb disposed at the bottom end of the pressure rod and a plurality of hooks fixedly disposed within the structural cavity and adapted to the barb.

[0007] Preferably, the pressure rod is a hollow structure, and a lifting rod is provided inside the pressure rod. The bottom of the lifting rod has a pair of dovetail-shaped spring arms, each of which extends towards the bottom of the pressure rod, and the barbs are respectively provided at the bottom end of the corresponding spring arms. The top of the pressure rod has a pressure head, which has a transverse through channel, and the channel has an unlocking button. Each unlocking button has a control ramp extending into the channel, and the top of each lifting rod has a control hole corresponding to each control ramp.

[0008] Preferably, a Western blot (WB) experimental method for the aforementioned protein blot fixation clip expander includes the following steps:

[0009] Step 1: Place the U-shaped silicone pad between the two glass slides to form a three-sided closed double glass plate pocket structure;

[0010] Step 2: Place the double glass plates on both sides of the electrophoresis tank and perform initial constraint by flipping the clamping cover; Step 3: By operating the pressure rod, the expansion bracket is squeezed against the double glass plates to enhance the sealing performance of the pocket structure.

[0011] Step 4: Place the electrophoresis tank inside the container for electrophoresis;

[0012] Step 5: Remove the double glass plate separation film and form a sandwich transfer mold structure;

[0013] Step 6: Place the sandwich mold structure on both sides of the electrophoresis tank and constrain it by flipping the clamping cap;

[0014] Step 7: Place the electrophoresis apparatus back into the container for transfer.

[0015] The beneficial effects of this invention are as follows: Under the action of the expansion mechanism, the two glass plates in the electrophoresis tank exert strong pressure on the U-shaped silicone pad, thereby improving the sealing performance of the pocket structure formed by the double glass plates and avoiding leakage of the electrophoretic solution. Specifically, the two assembled glass plates are placed in the positioning groove. By flipping the clamping cover, the clamping plate initially constrains the outer side of the glass plates. Then, the pressure rod is pressed down, and under the action of the gear transmission mechanism, the push rod pushes the silicone support frame to both sides to squeeze the glass plates outward, thereby further improving the sealing performance of the double glass plates, preventing leakage, and ensuring the sealing of the adhesive. The pressure rod is locked during the downward process by a locking structure, thereby preventing the pressure rod from rebounding and ensuring the long-term support force of the silicone support frame. The longitudinal displacement of the pressure rod is converted into the lateral displacement of the push rod through the transmission action of the gear mechanism, thereby meeting the driving force requirements of the silicone support frame. The locking mechanism uses the cooperation of hooks and barbs to implement a self-locking positioning effect on the pressure rod after it is pressed down. Once the pressure bar is locked, pressing the two unlocking buttons, under the action of the control ramp and control hole, moves the lifting rod upward, while the two spring arms at the bottom are pulled into the pressure bar, causing the two barbs to move inward and disengage from the hooks, thus unlocking the pressure bar. Compared to traditional Western blotting (WB) experiments, this innovative protein blot fixation clamp expander replaces the traditional gel preparation process. After placing the glass plate into the positioning groove in the fixation clamp expander, pressing the pressure head ensures the airtightness of the two glass plates during electrophoresis. Therefore, this device is a one-piece structure, allowing for direct addition of electrophoresis solution for electrophoresis. Using this structure, in the transfer step, the sandwich structure obtained after electrophoresis is placed in the positioning groove and fixed by the clamping cap, achieving a multi-purpose effect. Furthermore, the electrophoresis and transfer steps can be completed using a single container and electrophoresis tank, eliminating the need for traditional glass plate gel preparation devices. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 The clamping cover portion of this invention is in the open state;

[0019] Figure 3 This is a schematic diagram of the double-glass plate perspective structure of the present invention.

[0020] Figure 4This is a schematic diagram of the internal structure of the cavity in this invention;

[0021] Figure 5 This is a schematic diagram of the external structure of the container of the present invention;

[0022] Figure 6 This is a schematic diagram of the internal structure of the container of the present invention.

[0023] In the diagram: 1. Electrophoresis tank; 2. Structural cavity; 3. Expansion support; 4. Double glass plate; 5. Clamping cover; 6. Electrode; 7. Electrophoresis circuit; 8. Push rod; 9. U-shaped silicone pad; 10. Pressure rod; 11. Unlock button; 12. First rack; 13. First gear; 14. First bevel gear; 15. Second bevel gear; 16. Second gear; 17. Second rack; 18. Barb; 19. Hook; 20. Lifting rod; 21. Spring arm; 22. Control slope; 23. Control hole; 24. Container; 25. Mold transfer circuit; 26. Connector. Detailed Implementation

[0024] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0025] according to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6As shown, a protein blot fixation clip expander includes: an electrophoresis tank 1, with structural cavities 2 at both ends of the electrophoresis tank 1, each structural cavity 2 corresponding to an expansion mechanism on both sides of the electrophoresis tank 1, and a pressure rod 10 and an electrode 6 at the top of each structural cavity 2 to trigger the expansion mechanism, with an electrophoresis circuit 7 between two electrodes 6; an expansion support 3, disposed on both sides of the electrophoresis tank 1 and connected to the corresponding expansion mechanism, with a silicone strip on the outer side of each expansion support 3; and a double glass plate. 4. The double glass plate 4 is composed of two overlapping glass sheets, and a U-shaped silicone pad 9 is provided between the two glass sheets; the clamping cover 5 is provided in pairs and is respectively hinged to both ends of the electrophoresis tank 1, and is used to constrain the double glass plate 4 between the expansion bracket 3 and the clamping cover 5; the container 24 is used to carry the electrophoresis tank 1, and the inner walls of the container 24 on opposite sides are respectively provided with a mold transfer circuit 25, and each of the mold transfer circuits 25 is provided with a connector 26 extending outward through the side wall of the container 24. The expansion mechanism includes a push rod 8 corresponding to the expansion bracket 3, and a gear transmission mechanism disposed between the push rod 8 and the pressure rod 10. The gear transmission mechanism includes a first rack 12 disposed along the pressure rod 10, a first gear 13 meshing with the first rack 12, a first bevel gear 14 coaxially disposed on both sides of the first gear 13, a second bevel gear 15 meshing with the first bevel gear 14, a second gear 16 coaxially disposed at the bottom of the second bevel gear 15, and a second rack 17 disposed along the push rod 8, with the second gear 16 meshing with the second rack 17. The axis of the first gear 13 is parallel to the length direction of the push rod 8, and the axis of the second gear 16 is parallel to the length direction of the pressure rod 10. The expansion mechanism also includes a locking mechanism disposed within the structural cavity 2 and engaging with the bottom end of the pressure rod 10. The locking mechanism includes a barb 18 disposed at the bottom end of the pressure rod 10, and a plurality of hooks 19 fixedly disposed within the structural cavity 2 and adapted to the barb 18. The pressure rod 10 is a hollow structure, and a lifting rod 20 is provided inside the pressure rod 10. The bottom of the lifting rod 20 is provided with a pair of dovetail-shaped spring arms 21, each of which extends towards the bottom of the pressure rod 10, and the barbs 18 are respectively provided at the bottom end of the corresponding spring arms 21. The top of the pressure rod 10 is provided with a pressure head, which has a transverse through channel, and the channel is provided with an unlocking button 11. Each unlocking button 11 is provided with a control slope 22 extending into the channel, and the top of each lifting rod 20 is provided with a control hole 23 corresponding to each control slope 22.

[0026] Through the above setup, in order to improve the sealing effect of the double glass plates 4, the electrophoresis tank 1, under the action of the expansion mechanism, causes the two glass plates to forcefully compress the U-shaped silicone pad 9, thereby improving the sealing performance of the pocket structure formed by the double glass plates 4 and avoiding leakage of the electrophoretic solution. Specifically, after placing the combined two glass plates in the positioning groove, the clamping cover 5 is flipped to initially constrain the outer side of the glass plates. Then, the pressure rod 10 is pressed down, and under the action of the gear transmission mechanism, the push rod 8 is pushed to both sides to support the silicone support frame, thus squeezing the double glass plates 4 outward, further improving the sealing performance of the double glass plates 4, preventing leakage, and ensuring the sealing of the adhesive. The pressure rod 10 is locked during the downward process by a locking structure, thereby preventing the pressure rod 10 from rebounding and ensuring the sustained support force of the expansion bracket 3. Through the transmission action of the gear mechanism, the longitudinal displacement of the pressure rod 10 is converted into the lateral displacement of the push rod 8, thereby meeting the driving force requirements of the expansion bracket 3. The locking mechanism utilizes the cooperation of hook 19 and barb 18 to position and self-lock the depressed lever 10. Once the lever 10 is locked, pressing the two unlocking buttons 11 causes the lifting rod 20 to move upward under the action of the control ramp 22 and control hole 23, while the two spring arms 21 at the bottom are pulled into the lever 10, causing the two barbs 18 to move inward and disengage from the control of hook 19, thus completing the unlocking operation of the lever 10.

[0027] A Western blot experimental method based on this protein blot fixation clip expander includes the following steps:

[0028] Step 1: Place the U-shaped silicone pad 9 between the two glass slides to form a three-sided closed double glass plate pocket structure 4.

[0029] Step 2: Place the double glass plates 4 on both sides of the electrophoresis tank 1 and perform primary constraint by flipping the clamping cover 5; Step 3: By operating the pressure rod 10, the expansion bracket 3 is used to squeeze the double glass plates 4 to enhance the sealing performance of the pocket structure.

[0030] Step 4: Place the electrophoresis tank 1 inside the container 24 for electrophoresis;

[0031] Step 5: Remove the separation film from the double glass plate 4 and form a sandwich transfer mold structure;

[0032] Step 6: Place the sandwich mold structure on both sides of the electrophoresis tank 1 and constrain it by flipping the clamping cover 5;

[0033] Step 7: Place the electrophoretic material back into container 24 for mold transfer.

[0034] Compared to traditional Western blotting (WB) experiments, this innovative protein blot fixation clip expander adds a gel-forming function to the electrophoresis tank 1. After placing the glass plate into the positioning slot within the fixation clip expander, pressing the pressure head ensures a seal during both gel formation and electrophoresis. Therefore, this device integrates gel formation and electrophoresis; electrophoresis can be performed simply by adding electrophoresis solution to the tank after gel formation. Using this structure, the sandwich structure obtained after electrophoresis is placed in the positioning slot during the transfer step and fixed by the clamping cap 5, achieving a multi-purpose tank. Furthermore, the electrophoresis and transfer steps can be completed using a single container 24 and an electrophoresis tank 1, eliminating the need for traditional glass plate gel formation devices.

[0035] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A protein imprint fixation clip expander, characterized in that... ,include: An electrophoresis tank (1) is provided with structural cavities (2) at both ends of the electrophoresis tank (1). Each structural cavity (2) is provided with an expansion mechanism on both sides of the electrophoresis tank (1). Each structural cavity (2) is provided with a pressure rod (10) for triggering the expansion mechanism and an electrode (6) at the top. An electrophoresis circuit (7) is provided between the two electrodes (6). An expansion support (3) is provided on both sides of the electrophoresis tank (1) and connected to the corresponding expansion mechanism. A silicone strip is provided on the outer side of the expansion support (3). Double glass plate (4), the double glass plate (4) is composed of two overlapping glass sheets, and a U-shaped silicone pad (9) is provided between the two glass sheets; Clamping cover (5), the clamping cover (5) is arranged in pairs and is respectively hinged to both ends of the electrophoresis tank (1) to constrain the double glass plate (4) between the expansion bracket (3) and the clamping cover (5); A container (24) is used to carry the electrophoresis tank (1), and the inner walls of the container (24) on opposite sides are respectively provided with a mold transfer circuit (25), and each of the mold transfer circuits (25) is provided with a connector (26) extending outward through the side wall of the container (24).

2. The protein imprint fixation clip expander according to claim 1, characterized in that: The expansion mechanism includes a push rod (8) corresponding to the expansion bracket (3) and a gear transmission mechanism disposed between the push rod (8) and the pressure rod (10). The gear transmission mechanism includes a first rack (12) disposed along the pressure rod (10), a first gear (13) meshing with the first rack (12), a first bevel gear (14) coaxially disposed on both sides of the first gear (13), a second bevel gear (15) meshing with the first bevel gear (14), a second gear (16) coaxially disposed at the bottom of the second bevel gear (15), and a second rack (17) disposed along the push rod (8). The second gear (16) meshes with the second rack (17). The axis of the first gear (13) is parallel to the length direction of the push rod (8), and the axis of the second gear (16) is parallel to the length direction of the pressure rod (10).

3. The protein imprint fixation clip expander according to claim 2, characterized in that: The expansion mechanism also includes a locking mechanism disposed in the structural cavity (2) and cooperating with the bottom end of the pressure rod (10). The locking mechanism includes a barb (18) disposed at the bottom end of the pressure rod (10) and a plurality of hooks (19) fixedly disposed inside the structural cavity (2) and adapted to the barb (18).

4. The protein imprint fixation clip expander according to claim 3, characterized in that: The pressure rod (10) is a hollow structure, and a lifting rod (20) is provided inside the pressure rod (10). The bottom of the lifting rod (20) is provided with a pair of dovetail-shaped spring arms (21). Each spring arm (21) extends towards the bottom of the pressure rod (10), and the barbs (18) are respectively provided at the bottom end of the corresponding spring arms (21). The top of the pressure rod (10) is provided with a pressure head. The pressure head is provided with a transverse through channel, and the channel is provided with an unlocking button (11). Each unlocking button (11) is provided with a control slope (22) extending into the channel. The top of each lifting rod (20) is provided with a control hole (23) corresponding to each control slope (22).

5. A Western blot (WB) experimental method for the protein blot fixation clip expander as described in claims 1 to 4, characterized in that, Includes the following steps: Step 1: Place the U-shaped silicone pad (9) between the two glass slides to form a three-sided closed double glass plate (4) pocket structure; Step 2: Place the double glass plate (4) on both sides of the electrophoresis tank (1) and perform primary constraint by flipping the clamping cover (5); Step 3: By operating the pressure rod (10), the expansion bracket (3) is squeezed against the double glass plate (4) to enhance the sealing performance of the pocket structure. Step 4: Place the electrophoresis tank (1) in the container (24) for electrophoresis; Step 5: Remove the double glass plate (4), separate the adhesive film, and make a sandwich transfer mold structure; Step 6: Place the sandwich mold structure on both sides of the electrophoresis tank (1) and constrain it by flipping the clamping cover (5); Step 7: Place the electrophoretic material back into container (24) for mold transfer.

Images