A method for constructing a complete belt soup fingerprint spectrum and application thereof

Abstract

The application provides a method for constructing a complete belt soup fingerprint and application thereof, and the method comprises the following steps: (1) mixing the complete belt soup sample with an alcohol solution and ultrasonicating to obtain a test sample solution; (2) mixing the control sample with an alcohol solution to obtain a control sample solution; (3) respectively performing liquid phase detection on the test sample solution and the control sample solution, screening out the control sample peaks and peaks with good peak shape and high separation degree as characteristic peaks in the test sample solution according to the results, selecting the S peak with good peak shape and determined composition from the characteristic peaks, and calculating the relative retention time of other characteristic peaks relative to the S peak, so that the complete belt soup fingerprint is obtained. The fingerprint obtained by the method can effectively and accurately identify the quality of the complete belt soup, and the attribution of each peak in the characteristic fingerprint is determined, the specificity is strong, and the accuracy of quality control can be effectively improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of traditional Chinese medicine quality control, and particularly relates to a construction method of a complete band decoction fingerprint spectrum and application thereof, and particularly relates to an accurate construction method of a complete band decoction fingerprint spectrum and application thereof. BACKGROUND

[0002] The complete band decoction is derived from Fu Qingzhu's Notes on Women's Diseases, Volume 1, Leukorrhagia (Fu Shan, Qing Dynasty), and is composed of white atractylodes (stir-fried), Chinese yam (stir-fried), ginseng, white peony root (stir-fried with wine), plantago seed (stir-fried with wine), Chinese atractylodes (processed), dried tangerine or orange peel, black mustard, licorice, and bupleurum. In terms of prescription compatibility, white atractylodes is sweet and bitter and warm, tonifying qi and invigorating the spleen, drying dampness and promoting diuresis; Chinese yam is sweet and flat, invigorating the spleen and tonifying the middle, and can also tonify the kidney and astringe leukorrhagia; both drugs are used in large amounts, aiming to tonify and eliminate dampness, so that the spleen qi is invigorated and dampness is eliminated, and they are the monarch drugs. Ginseng greatly tonifies primordial qi, tonifying the spleen and invigorating the middle, enhancing the tonifying effect of the monarch drugs on the spleen; Chinese atractylodes dries dampness and transports the spleen, assisting the monarch drugs in eliminating dampness and resolving turbidity; plantago seed clears water and dampness, allowing dampness to be discharged from the water channel; white peony root astringes the liver and makes the liver soft, so that the spleen is invigorated; the above four drugs are used in moderate amounts and are the minister drugs. Dried tangerine or orange peel regulates qi, dries dampness, and invigorates the spleen, and is combined with white atractylodes, Chinese yam, and ginseng to make tonification without stagnation; bupleurum and mustard are used to ascend and disperse, and are combined with white atractylodes and ginseng to ascend and release clear yang, and are combined with white peony root to resolve stagnation, and the black mustard is stir-fried and used in a small amount, which can enter the blood and eliminate dampness, and regulate qi and blood; the above three drugs are used in small amounts, and are mainly used to soothe the liver and regulate the spleen, and are mainly used to regulate qi and blood, and are the assistant drugs. Licorice regulates all drugs. After the drugs are combined, they can tonify qi, invigorate the spleen, soothe the liver, regulate qi, and resolve dampness and leukorrhagia.

[0003] Traditional Chinese medicines and preparations are complex multi-component systems, so their quality should be evaluated by a method suitable for them. However, most of the existing methods are only for the research of single components or single types of components, and there are few researches on whole compound decoctions. As a compound preparation, the complete band decoction is a mixture of complex chemical components, and the effective components include volatile oil, saponins, terpenes, flavonoids, etc., so it is necessary to detect the chemical components, and therefore, how to provide a method for effectively controlling the main components of the complete band decoction and controlling the quality of the complete band decoction has become a problem to be solved. SUMMARY

[0004] In view of the deficiencies of the prior art, the purpose of the present application is to provide a construction method of a complete band decoction fingerprint spectrum and application thereof, and particularly to provide an accurate construction method of a complete band decoction fingerprint spectrum and application thereof. The fingerprint spectrum obtained by the construction method provided by the present application can effectively and accurately identify the quality of the complete band decoction, and the attribution of each peak in the characteristic spectrum is performed, which is highly specific and can effectively improve the accuracy of quality control.

[0005] To achieve the purpose of the present application, the following technical solutions are adopted:

[0006] In one aspect, the present application provides a method for constructing a complete belt soup fingerprint, comprising the following steps:

[0007] (1) mixing the complete belt soup sample with an alcohol solution by ultrasonic, to obtain a test sample solution;

[0008] (2) mixing the control sample with an alcohol solution to obtain a control sample solution, wherein the control sample comprises any one or a combination of at least two of allantoin, gallic acid, geniposidic acid, chlorogenic acid, paeonilactoside, paeoniflorin, vitexin-2"-O- glucoside, glycyrrhizin, verbascoside, hesperidin, benzoylpaeoniflorin, glycyrrhizic acid, nobiletin, atractylenolide III, tangeretin or atractylenolide II;

[0009] (3) detecting the test sample solution and the control sample solution by liquid chromatography, screening the characteristic peaks with good peak shape and high separation degree from the test sample solution according to the results, selecting the S peak with good peak shape and component determination from the characteristic peaks, and calculating the relative retention time of other characteristic peaks relative to the S peak, to obtain the complete belt soup fingerprint.

[0010] The steps (1) and (2) are not distinguished in order.

[0011] The present application can effectively establish the fingerprint of the complete belt soup by detecting and comparing the specific control sample with the test sample, and effectively identify the quality of the complete belt soup.

[0012] Preferably, the alcohol solution is a methanol aqueous solution, and the volume fraction of the methanol aqueous solution is 30-90%.

[0013] Preferably, the ratio of the complete belt soup sample to the alcohol solution in step (1) is (0.5-1.5):(10-50) g / mL.

[0014] Preferably, when the control sample comprises at least two kinds, each control sample is prepared into a single standard control sample solution, and the concentration of the single standard control sample solution is 20-100 μg / mL.

[0015] The volume fraction of the methanol aqueous solution can be 30%, 40%, 50%, 60%, 70%, 80%, or 90%, etc. In the liquid ratio of the complete decoction soup sample and the alcohol solution, the fraction of the complete decoction soup sample can be 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5, etc. The fraction of the alcohol solution can be 10, 15, 20, 25, 30, 35, 40, 45, or 50, etc. The concentration of the single standard control solution can be 20 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL, 60 μg / mL, 70 μg / mL, 80 μg / mL, 90 μg / mL, or 100 μg / mL, etc. However, the above listed values are not limiting, and other values within the above range are also applicable.

[0016] Preferably, before the step (3), the method further comprises mixing and decocting the control medicinal materials with water respectively to obtain control medicinal material solutions, wherein the control medicinal materials include Chinese yam, psyllium, white peony root, licorice, white atractylodes, bupleurum, dried tangerine or orange peel, schizonepeta, ginseng, and atractylodes lancea.

[0017] Preferably, the step (3) further comprises performing liquid phase detection on the control medicinal material solutions, combining the detection results with the characteristic peaks, and corresponding attributing the characteristic peaks to the control medicinal materials.

[0018] Preferably, the chromatographic column for the liquid phase detection is a C18 chromatographic column, and the column temperature is 25-35°C.

[0019] Preferably, the mobile phase for the liquid phase detection includes mobile phase A and mobile phase B, the mobile phase A is acetonitrile, and the mobile phase B is phosphoric acid aqueous solution, and the mass fraction of the phosphoric acid aqueous solution is 0.08-0.12%, for example, 0.08%, 0.09%, 0.1%, 0.11%, or 0.12%, etc. However, the above listed values are not limiting, and other values within the above range are also applicable.

[0020] Preferably, the liquid phase detection adopts gradient elution, and the gradient elution is specifically as follows:

[0021] From 0-9 min, the volume fraction of the mobile phase A is 0, the volume fraction of the mobile phase B is 100%, and the flow rate is 0.4 mL / min;

[0022] From 9-10 min, the volume fraction of the mobile phase A is 0, the volume fraction of the mobile phase B is 100%, and the flow rate is uniformly changed from 0.4 mL / min to 1 mL / min;

[0023] From 10-70 min, the volume fraction of the mobile phase A is uniformly changed from 0 to 30%, the volume fraction of the mobile phase B is uniformly changed from 100% to 70%, and the flow rate is 1 mL / min;

[0024] 70-100min, the volume fraction of mobile phase A is uniformly changed from 30% to 90%, the volume fraction of mobile phase B is uniformly changed from 70% to 10%, and the flow rate is 1 mL / min.

[0025] Preferably, in the characteristic spectrum, the number of characteristic peaks is 19, which are sorted in ascending order of retention time, and the peak No. 9 is the S peak, and the relative retention time of peak No. 1-8, 10-19 is 0.165-0.183, 0.454-0.502, 0.509-0.563, 0.604-0.668, 0.705-0.779, 0.800-0.884, 0.817-0.903, 0.908-1.004, 1.079-1.193, 1.088-1.202, 1.095-1.211, 1.194-1.320, 1.511-1.671, 1.581-1.747, 1.682-1.860, 1.703-1.883, 1.729-1.911, 1.804-1.994.

[0026] The peak No. 9 component is paeoniflorin.

[0027] Preferably, peak No. 1 belongs to yam, peak No. 2, 6, 8, 9, 14 belongs to white peony, peak No. 4, 12 belongs to plantago, peak No. 10, 11, 15 belongs to licorice slice, peak No. 13, 16, 18 belongs to dried tangerine or orange peel, peak No. 17, 19 belongs to white atractylodes, and peak No. 3, 5, 7 does not have specificity.

[0028] On the other hand, the application also provides a quality control method of perfect belt soup, different batches of perfect belt soup are detected by the liquid phase detection method, the detection results are processed by median number, and the standard characteristic spectrum is obtained by combining the characteristic spectrum; the sample to be detected is detected by the liquid phase detection method, the characteristic peaks are judged according to the characteristic spectrum, and then the similarity is judged, and the sample to be detected is judged as a qualified product or an unqualified product.

[0029] In the similarity judgment, the similarity of the characteristic peaks of the sample to be detected and the characteristic peaks in the standard characteristic spectrum is calculated; when the similarity is not less than 0.9, the sample to be detected is a qualified product, and when the similarity is less than 0.9, the sample to be detected is an unqualified product.

[0030] Compared with the prior art, the application has the following beneficial effects:

[0031] The application provides a method for constructing a complete belt soup fingerprint, which can effectively establish the fingerprint of the complete belt soup by detecting and comparing specific reference substances with test samples, realizes effective identification of the quality of the complete belt soup, and establishes a reference relationship between characteristic peaks and each medicinal ingredient in the complete belt soup, has clear specificity, and can further improve the accuracy of quality control. BRIEF DESCRIPTION OF DRAWINGS

[0032] Figure 1 Figure 1 is a liquid chromatography detection result diagram of the test sample solution in Example 1, wherein 1-19 respectively represent characteristic peaks 1-19. DETAILED DESCRIPTION

[0033] The technical solutions of the application are further described below through specific embodiments. Those skilled in the art should understand that the embodiments are only used to help understand the application and should not be regarded as specific limitations on the application.

[0034] Instruments and reagents

[0035] XRP2 electronic analytical balance (Switzerland Mettler-Toledo Corporation), ML204T / 02 electronic analytical balance (Switzerland Mettler-Toledo Corporation), KQ-250DB numerical control ultrasonic cleaning instrument (Kunshan Ultrasonic Instrument Co., Ltd.), DHG-9070A electric heating constant temperature air drying oven (Shanghai Xinmiao Medical Instrument Manufacturing Co., Ltd.), LC-2030C3D Plus high performance liquid chromatograph (Shimadzu Technology Co., Ltd.), Milli-Q (IQ-7000) pure water instrument (Millipore Corporation, USA).

[0036] Sample: complete belt soup reference sample (take 37g of fried Chinese yam, 11g of wine caraway, 11g of wine white peony root, 3g of liquorice tablet, 37g of earth white atractylodes, 2g of bupleurum, 2g of dried tangerine or orange peel, 2g of carburized schizonepeta, 7g of ginseng, 11g of bran-fried atractylodes, and the above ten medicinal materials are placed in a decoction kettle, and then pure water is added for decoction, filtration, cooling of the filtrate to 20℃, rotary evaporation concentration of the concentrated medicinal liquid, and program freeze-drying of the concentrated medicinal liquid to obtain the complete belt soup reference sample powder).

[0037] Reference substance: paeonol reference substance (batch number: 110736-201943, content is calculated according to 95.1%), purchased from China Institute for Drug Control;

[0038] Paeonol reference substance (batch number: JZ19011502, content is calculated according to 98%), purchased from Nanjing Cassels Pharmaceutical Technology Co., Ltd.;

[0039] Paeonol reference substance (batch number: PS000157, content is calculated according to 98%), purchased from Chengdu Pus Biological Technology Co., Ltd.;

[0040] Allantoin Reference Standard (Batch No. 111501-200202) was purchased from China National Institute for Food and Drug Control;

[0041] Geniposidic Acid Reference Standard (Batch No. 111828-201805, content 98.1%) was purchased from China National Institute for Food and Drug Control;

[0042] Verbascoside Reference Standard (Batch No. 111530-201914, content 95.2%) was purchased from China National Institute for Food and Drug Control;

[0043] Hesperidin Reference Standard (Batch No. 110721-201818, content 96.2%) was purchased from China National Institute for Food and Drug Control;

[0044] Hesperitin (Crocetin) Reference Standard (Batch No. PS010637, content ≥98.0%) was purchased from Chengdu Pus Bio-tech Co., Ltd;

[0045] Nobiletin Reference Standard (Batch No. 8005, content 99.1%) was purchased from Shanghai Shidand Standard Technology Service Co., Ltd;

[0046] Atractylodin Reference Standard (Batch No. 111978-201501, content 99.9%) was purchased from China National Institute for Food and Drug Control;

[0047] Atractylodin Reference Standard (Batch No. 111976-201501, content 99.9%) was purchased from China National Institute for Food and Drug Control;

[0048] Gallic Acid Reference Standard (Batch No. 1110831-201906, content 91.5%) was purchased from China National Institute for Food and Drug Control;

[0049] Glycyrrhizin Reference Standard (Batch No. PS011457, content >98.0%) was purchased from Chengdu Pus Bio-tech Co., Ltd;

[0050] Glycyrrhizin Reference Standard (Batch No. 111610-201908, content 95.0%) was purchased from China National Institute for Food and Drug Control;

[0051] Glycyrrhizin Reference Standard (Batch No. 111610-201908, content 95.0%) was purchased from China National Institute for Food and Drug Control;

[0052] Chlorogenic Acid Reference Standard (Batch No. 110753-202018, content 96.1%) was purchased from China National Institute for Food and Drug Control

[0053] Reagents: Acetonitrile was chromatographically pure (produced by DikMA company); water was ultrapure water; phosphoric acid was chromatographically pure, produced by laddin; other reagents were analytically pure.

[0054] Example 1

[0055] The present embodiment provides a method for constructing a complete fingerprint of the complete belt soup, and the specific steps are as follows:

[0056] Take 1 g of complete belt soup reference sample powder, accurately weigh, place in a conical flask with a plug, accurately add 25 mL of 60% methanol solution, tightly plug, weigh, ultrasonic treatment (power 250 W, frequency 40 kHz) for 30 minutes, cool, weigh again, make up the weight loss with 60% methanol solution, shake well, filter to obtain the test solution.

[0057] Take 1 g of complete belt soup reference sample powder, accurately weigh, place in a conical flask with a plug, accurately add 25 mL of 60% methanol solution, tightly plug, weigh, ultrasonic treatment (power 250 W, frequency 40 kHz) for 30 minutes, cool, weigh again, make up the weight loss with 60% methanol solution, shake well, filter to obtain the test solution.

[0058] Take 1 g of complete belt soup reference sample powder, accurately weigh, place in a conical flask with a plug, accurately add 25 mL of 60% methanol solution, tightly plug, weigh, ultrasonic treatment (power 250 W, frequency 40 kHz) for 30 minutes, cool, weigh again, make up the weight loss with 60% methanol solution, shake well, filter to obtain the test solution.

[0059] The above test solution, single standard control solution and single standard control medicinal material solution were subjected to liquid phase detection (repeated three times, and the test results of the test solution are shown in Table 1-1, wherein 1-19 represent characteristic peaks 1-19), and the parameters are as follows: Figure 1

[0060] The gradient elution program is as follows:

[0061]

[0062] The column temperature was 30℃, the injection amount was 10 μL, the chromatographic column was Shim-pack GIST C18-AQ (S / N: 19L09745) chromatographic column, and the detection wavelength was 210 nm.

[0063] According to the liquid phase results, 19 characteristic peaks were screened, and the peaks were sorted in ascending order of retention time, with peak 9 as S peak (paeoniflorin), and the parameters of the remaining peaks are as follows:

[0064] ​

[0065]

[0066] The peaks were assigned, wherein peak 1 was assigned to yam, peaks 2, 6, 8, 9, 14 were assigned to white peony root, peaks 4, 12 were assigned to plantain seed, peaks 10, 11, 15 were assigned to licorice tablet, peaks 13, 16, 18 were assigned to dried tangerine or orange peel, peaks 17, 19 were assigned to white atractylodes, and peaks 3, 5, 7 were not specific.

[0067] Elution program selection:

[0068] Based on the method of Example 1, the elution program was replaced by elution programs 1-3 (see table below), respectively, and then liquid phase detection was performed.

[0069] Elution program 1

[0070]

[0071] Elution program 2

[0072]

[0073]

[0074] Elution program 3

[0075]

[0076] Compared with Example 1, the results showed that the number of chromatographic peaks was less and the separation effect was poorer when the chromatographic elution programs 1-3 were used for detection.

[0077] Mobile phase selection:

[0078] Based on the method of Example 1, the mobile phase B was replaced by 0.1% formic acid solution and 0.1% trifluoroacetic acid solution, respectively, and then liquid phase detection was performed.

[0079] Compared with Example 1, when the mobile phase B was replaced by 0.1% formic acid solution system, the baseline shift of the chromatographic peaks was more serious, and the main chromatographic peaks did not achieve good separation effect; when the mobile phase B was replaced by 0.1% trifluoroacetic acid solution system, the baseline shift of the chromatographic peaks was more serious, and the main chromatographic peaks did not achieve good separation effect.

[0080] Durability investigation:

[0081] Based on the method of Example 1, the chromatographic column was replaced by Thermo Acclaim TM 120 C18, 4.6 x 250 mm, 5 μm (S / N: 016248) and Shim-pack GIST C18-AQ, 4.6 x 250 mm, 5 μm (S / N: 20A00696) respectively, and then liquid phase detection was carried out.

[0082] It is compared with Example 1. The results of the three all reflect excellent separation effect, which indicates that the method has good durability.

[0083] Methodology investigation (the following test methods are based on Example 1)

[0084] Precision:

[0085] The test sample solution was prepared according to the preparation method of the test sample solution of Example 1 after the standard sample with soup was taken, and 5 times of continuous injection were determined. The results show that the relative standard deviation of the relative retention time of each common peak in the test sample solution is less than 3%, and the similarity is not less than 0.999, which indicates that the method has good precision.

[0086]

[0087]

[0088]

[0089] Repeatability:

[0090] The test sample solution was prepared according to the preparation method of the test sample solution of Example 1 after the standard sample with soup was taken, and 5 times of continuous injection were determined. The results show that the relative standard deviation of the relative retention time of each common peak in the test sample solution is less than 3%, and the similarity is not less than 0.999, which indicates that the method has good precision.

[0091]

[0092]

[0093] Stability:

[0094] The test sample solution was prepared according to the preparation method of the test sample solution of Example 1 after the standard sample with soup was taken, and 5 times of continuous injection were determined. The results show that the relative standard deviation of the relative retention time of each common peak in the test sample solution is less than 3%, and the similarity is not less than 0.999, which indicates that the method has good precision.

[0095]

[0096]

[0097] Effect verification:

[0098] The method and characteristic spectrum provided by Example 1 and the method and characteristic spectrum of Example 1 without detecting the reference solution, only detecting the test solution and the control medicinal material solution (control group) are used to discriminate the sample to be tested. The true product result of Example 1 is as follows:

[0099]

[0100] The pseudo product lacks characteristic peak 1, so it can be found that the method provided by the application can effectively realize the true and false identification of complete decoction.

[0101] The true product result of the control group is as follows:

[0102]

[0103]

[0104] However, there is no characteristic peak missing in the pseudo product, and the true and false identification cannot be realized.

[0105] The above data shows that the method provided by the application can effectively identify the quality of complete decoction, and by establishing the control relationship between the characteristic peaks and each medicinal material in the complete decoction, the true and false of the sample to be tested can be effectively distinguished.

[0106] The applicant declares that the application of the application is illustrated by the above examples, but the application is not limited to the above examples, that is, it does not mean that the application must rely on the above examples to be implemented. Those skilled in the art should understand that any improvement of the application, equivalent replacement of each raw material of the product of the application, addition of auxiliary ingredients, selection of specific modes, etc. fall within the protection scope and disclosure scope of the application.

[0107] The above detailed the preferred embodiments of the application, but the application is not limited to the specific details in the above embodiments, and within the technical concept range of the application, the technical solutions of the application can be variously modified, and these simple modifications all belong to the protection scope of the application.

[0108] In addition, it should be noted that each specific technical feature described in the above specific embodiments can be combined in any appropriate manner without contradiction, and in order to avoid unnecessary repetition, the application will not further describe various possible combination manners.

Claims

1. A method for constructing a complete bandgap fingerprint spectrum, characterized in that, The construction method includes the following steps: (1) Mix the whole sample with the alcohol solution and sonicate to obtain the test solution; (2) The reference standard is mixed with an alcohol solution to obtain a reference standard solution, wherein the reference standard includes allantoin, gallic acid, genipin, chlorogenic acid, paeoniflorin, paeoniflorin, apigenin, glycyrrhizin, verbascoside, hesperidin, benzoylpaeoniflorin, glycyrrhizic acid, tangeretin, atractylodes lactone III, hesperidin and atractylodes lactone II; (3) Perform liquid chromatography detection on the test solution and the reference solution respectively. Based on the results, select the reference peak and the peak with good peak shape and high resolution as characteristic peaks from the liquid chromatography detection results of the test solution. Select the characteristic peak with a definite composition and good peak shape as the S peak. Calculate the relative retention time of other characteristic peaks relative to the S peak to obtain the complete fingerprint spectrum. Steps (1) and (2) are not in any particular order; The mobile phase for liquid chromatography detection includes mobile phase A and mobile phase B. Mobile phase A is acetonitrile, and mobile phase B is an aqueous solution of phosphoric acid with a mass fraction of 0.08-0.12%. The chromatographic column for liquid chromatography detection is a C18 column with a column temperature of 25-35℃ and a detection wavelength of 210 nm. The liquid phase detection employs gradient elution, which is specifically described below: From 0 to 9 min, the volume fraction of mobile phase A was 0, the volume fraction of mobile phase B was 100%, and the flow rate was 0.4 mL / min. At 9-10 min, the volume fraction of mobile phase A was 0, the volume fraction of mobile phase B was 100%, and the flow rate changed from a constant 0.4 mL / min to 1 mL / min. From 10 to 70 min, the volume fraction of mobile phase A was changed from 0 to 30% at a constant rate, and the volume fraction of mobile phase B was changed from 100% to 70% at a constant rate, with a flow rate of 1 mL / min. Between 70 and 100 min, the volume fraction of mobile phase A was changed from 30% to 90% at a constant rate, and the volume fraction of mobile phase B was changed from 70% to 10% at a constant rate, with a flow rate of 1 mL / min.

2. The construction method according to claim 1, characterized in that, The alcohol solution is an aqueous methanol solution with a volume fraction of 30-90%.

3. The construction method according to claim 1, characterized in that, In step (1), the ratio of the sample to the alcohol solution in the whole soup is (0.5-1.5):(10-50) g / mL.

4. The construction method according to claim 1, characterized in that, When the reference standard in step (2) includes at least two types, each reference standard is prepared into a single-standard reference solution with a concentration of 20-100 μg / mL.

5. The construction method according to claim 1, characterized in that, Before step (3), the reference medicinal materials are mixed with water and decocted to obtain a reference medicinal material solution. The reference medicinal materials include yam, plantain seed, white peony root, licorice, atractylodes macrocephala, bupleurum, tangerine peel, schizonepeta spike, ginseng and atractylodes lancea.

6. The construction method according to claim 5, characterized in that, Step (3) further includes performing liquid phase detection on the control medicinal material solution, combining the detection results with the characteristic peaks, and assigning the characteristic peaks to the control medicinal materials.

7. The construction method according to claim 1, characterized in that, The fingerprint spectrum contains 19 characteristic peaks, ordered from smallest to largest by retention time. Peak 9 is designated as peak S. The relative retention times of peaks 1-8 and 10-19 are 0.165-0.183, 0.454-0.502, 0.509-0.563, 0.604-0.668, 0.705-0.779, 0.800-0.884, and 0.817-, respectively. 0.903, 0.908-1.004, 1.079-1.193, 1.088-1.202, 1.095-1.211, 1.194-1.320, 1.511-1.671, 1.581-1.747, 1.682-1.860, 1.703-1.883, 1.729-1.911, 1.804-1.994; The component of peak number 9 is paeoniflorin.

8. The construction method according to claim 7, characterized in that, Peak 1 belongs to Dioscorea opposita, peaks 2, 6, 8, 9, and 14 belong to Paeonia lactiflora, peaks 4 and 12 belong to Plantago asiatica, peaks 10, 11, and 15 belong to Glycyrrhiza uralensis, peaks 13, 16, and 18 belong to Citrus reticulata, peaks 17 and 19 belong to Atractylodes macrocephala, and peaks 3, 5, and 7 do not have specific attributes.

9. A method for quality control of soup, characterized in that, Different batches of Wan Dai Tang were tested using the Wan Dai Tang fingerprint spectrum construction method according to any one of claims 1-8. The test results were processed by median and combined with the feature spectrum to obtain a standard feature spectrum. The sample to be tested was tested using any one of the liquid chromatography detection methods according to claims 1-8. The characteristic peaks were determined according to the feature spectrum, and then the similarity was judged to determine whether the sample to be tested was qualified or unqualified. In the similarity judgment, the similarity between the characteristic peak of the sample to be tested and the characteristic peak in the standard characteristic spectrum is calculated; if the similarity is not less than 0.9, the sample to be tested is qualified; if the similarity is less than 0.9, the sample to be tested is unqualified.

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