A pesticidal composition and its application

Abstract

The present invention belongs to the technical field of pesticidal acaricides, and discloses a pesticidal composition and its application. The active ingredients in the pesticidal composition include active ingredient A and active ingredient B. The active ingredient A is sulfiflumin, and the active ingredient B is any one of azocyclotin or fenbutatin oxide. The pesticidal composition of the present invention has the advantages of significant synergistic effect on mite pests, slowing down the development of drug resistance, reducing the amount of pesticide used, etc.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticide acaricides, and discloses a pesticide composition and its application. Background Art

[0002] Azocyclotin, with a CAS registry number of 41083-11-8 and a chemical name of tricyclohexyl(1,2,4-triazol-1-yl)stannane, is a broad-spectrum systemic acaricide with a wide acaricidal spectrum, high activity, and safety to crops. It can control various harmful mites such as Panonychus citri, Tetranychus urticae, and Phyllocoptruta oleivora on crops such as citrus, hawthorn, apple, grape, and eggplant, and has good control effects on nymphs, adults, and eggs.

[0003] Fenbutatin oxide, with a CAS registry number of 13356-08-6 and a chemical name of bis[tris(2-methyl-2-phenylpropyl)tin]oxide, is an efficient temperature-sensitive acaricide. It is mainly used for controlling mite damage on citrus, apple, grape, tea, solanaceous plants, and ornamental flowers. It can effectively kill Panonychus citri, Tetranychus urticae, Phyllocoptruta oleivora, Brevipalpus obovatus, and Oligonychus punicae, etc., and has the characteristics of high efficiency, low toxicity, safety, long residual period, and no resistance occurrence.

[0004] Mites are a type of harmful organisms that are relatively difficult to control in agricultural production. Especially due to the improper use of pesticides, Tetranychus urticae has risen from a secondary pest to a major pest, showing a trend of increasing year by year in China.

[0005] Mixing or rotating acaricides with different action mechanisms is an important means to delay the resistance of harmful mites and its management, and at the same time has the effects of reducing toxicity and cost. The compound agent with synergistic effect can not only reduce the dosage of pesticides, but also enhance the control effect, and can further delay the resistance of harmful mites. Therefore, the applicant combines sulfiflumin with any one of azocyclotin or fenbutatin oxide to develop a highly efficient compound acaricide with multiple action mechanisms, providing a theoretical basis for the management of mite resistance and further improving the control effect of harmful mites. Summary of the Invention

[0006] Based on the above content, the object of the present invention is to provide a pesticide composition. The pesticide composition of the present invention has excellent control effects on harmful mites such as citrus leaf mites and apple leaf mites, and has a significant synergistic effect;

[0007] Another object of the present invention is to provide a pesticide composition that is safe for crops;

[0008] To achieve the above object, the present invention adopts the following technical solution. A pesticide composition, wherein the active ingredients of the pesticide composition comprise active ingredient A and active ingredient B. The active ingredient A is sulfiflumin, and the active ingredient B is any one of azocyclotin or fenbutatin oxide. The mass ratio of active ingredient A to active ingredient B in the pesticide composition is 1:45 to 35:1.

[0009] Further, the active ingredient B in the pesticide composition is azocyclotin, and the mass ratio of active ingredient A to active ingredient B is 1:40 to 30:1;

[0010] Even further, the active ingredient B in the pesticide composition is azocyclotin, and the mass ratio of active ingredient A to active ingredient B is 1:30 to 20:1;

[0011] Further, the active ingredient B in the pesticide composition is fenbutatin oxide, and the mass ratio of active ingredient A to active ingredient B is 1:45 to 35:1;

[0012] Even further, the active ingredient B in the pesticide composition is fenbutatin oxide, and the mass ratio of active ingredient A to active ingredient B is 1:35 to 25:1.

[0013] Further, in addition to the active ingredients, the pesticide composition further comprises agriculturally acceptable auxiliary ingredients, and the auxiliary ingredients are fillers and / or surfactants;

[0014] Further, the surfactants are wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, preservatives, stabilizers, synergists;

[0015] Further, the wetting agent is one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soap pod powder, sapindus powder, SOPA, detergent, emulsifier 2000 series, and wetting penetrant F; and / or

[0016] Further, the dispersant is one or more of lignin sulfonates, alkylnaphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensates sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylates, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0017] Further, the emulsifier is one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0018] Further, the thickener is one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica white; and / or

[0019] Further, the disintegrant is one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0020] Further, the antifreeze is one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0021] Further, the defoamer is one or more of C 10 -C 20 saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 fatty alcohols; and / or

[0022] Further, the preservative is one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0023] Further, the stabilizer is one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica white, talc powder, montmorillonite, and starch; and / or

[0024] Further, the synergist is selected from synergistic phosphorus and synergistic ether; and / or

[0025] Further, the filler is a solvent, a filler, or a carrier;

[0026] Further, the solvent is one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oil, vegetable oil derivatives, and water; and / or

[0027] Further, the carrier is one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0028] Further, the formulation form of the pesticide composition is a solution, a suspension, an emulsifiable concentrate, an emulsion in water, a microcapsule suspension, a microemulsion, a suspo-emulsion, a dispersible oil suspension, a granule, a water-dispersible granule, or a wettable powder;

[0029] Furthermore, the formulation form is a suspension, an emulsifiable concentrate, a water-dispersible granule, or a wettable powder.

[0030] The present invention discloses the use of the pesticide composition as described above for controlling pest mites.

[0031] Further, the pest mites are phytophagous mites;

[0032] Further, the pest mites are citrus red mites or apple red mites;

[0033] Furthermore, the citrus red mite is Panonychus citri, and the apple red mite is Panonychus ulmi or Tetranychus viennensis. [[ID=@19]]

[0034] Further, the pesticide composition is applied to pest mites and the plants on which they grow or the environment in which they grow.

[0035] The beneficial effects of the present invention are as follows:

[0036] 1) The pesticide composition of the present invention rationally combines acaricides with different action mechanisms, has a significant synergistic effect on citrus or apple leaf mites, and has a good control effect;

[0037] 2) The pesticide composition of the present invention does not cause phytotoxicity to target plants, is safe for natural enemies, and effectively reduces the amount of pesticide used. Detailed implementation manners

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in combination with the preparation examples and specific examples of the formulations of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0039] Preparation Example 1: 18% sulfiflumin·azocyclotin wettable powder (1:8) [[ID=@40]]

[0040] Formulation composition: 2% sulfiflumin, 16% azocyclotin, 2.5% sodium dodecyl sulfate, 4% sodium methylnaphthalene sulfonate formaldehyde condensate, 3% Nekal BX, 5% silica white, and kaolin to make up the balance;

[0041] Preparation method: According to the formula ratio, mix the active ingredient, dispersant, wetting agent and filler, stir evenly in a stirring kettle, and perform multiple pulverizations and mixings evenly by an air-flow pulverizer to prepare the wettable powder of the composition of the present invention.

[0042] Preparation Example 2: 21% sulfiflumin·azocyclotin water-dispersible granule (6:1)

[0043] Formulation composition: 18% sulfiflumin, 3% azocyclotin, 8% succinate sulfonate, 5% sodium lignosulfonate, 2% sodium dodecyl sulfate, 5% silica white, 25% starch, kaolin to make up the balance;

[0044] Preparation method: According to the formula ratio, add the active ingredient to the carrier, add surfactants and other functional auxiliaries thereto, mix, add 10-25% of water after air-flow pulverization, and then obtain the water-dispersible granule product through kneading, granulation, drying and screening; or spray water, granulate and dry the pulverized powder in a fluidized granulator, and then screen to obtain the product.

[0045] Preparation Example 3: 20% sulfiflumin·azocyclotin suspension concentrate (3:1)

[0046] Formulation composition: 15% sulfiflumin, 5% azocyclotin, 1% polyoxyethylene ether of gerb alcohol, 5% polyoxyethylene phenylphenol phosphate, 1% sodium lignosulfonate, 0.2% xanthan gum, 5% ethylene glycol, 0.1% potassium benzisothiazolinone, 0.5% silicone oil, deionized water to make up the balance;

[0047] Preparation method: According to the formula ratio, place the active ingredient, surfactant and other functional auxiliaries in a reaction kettle in sequence, add water and mix evenly, perform high-speed shearing and wet grinding, and finally perform homogeneous filtration to obtain the suspension concentrate product.

[0048] Preparation Example 4: 24% sulfiflumin·azocyclotin emulsifiable concentrate (5:1)

[0049] Formulation composition: 20% sulfiflumin, 4% azocyclotin, 16% DMF, 12% polyoxyethylene isomeric tridecyl alcohol ether, 1% calcium dodecylbenzenesulfonate, 18% propylene carbonate, xylene to make up the balance;

[0050] Preparation method: According to the formula ratio, add the active ingredient, solvent and cosolvent to a blending kettle and stir to dissolve, then add the emulsifier, make up the balance with the remaining solvent, stir evenly in a stirring kettle, and filter to obtain the emulsifiable concentrate required by the present invention.

[0051] Preparation Example 5: 16% Sulfiflumin·Fenbutatin Oxide Wettable Powder (3:1)

[0052] Formulation composition: 12% Sulfiflumin, 4% Fenbutatin Oxide, 5% Sodium Lignosulfonate, 8% Naphthalene Sulfonate Formaldehyde Condensate, 5% Nekal BX, Kaolin to make up the balance;

[0053] Preparation method: The same as Preparation Example 1.

[0054] Preparation Example 6: 25% Sulfiflumin·Fenbutatin Oxide Suspension Concentrate (1:4)

[0055] Formulation composition: 5% Sulfiflumin, 20% Fenbutatin Oxide, 2% Guerbet Alcohol Polyoxyethylene Ether, 2% Alkyl Naphthalene Sulfonate, 4% Styrenated Phenol Polyoxyethylene Ether Phosphate, 2% Phenethylphenol Polyoxyethylene Polyoxypropylene Ether, 0.2% Xanthan Gum, 1% Magnesium Aluminum Silicate, 5% Glycerol, 0.2% Potassium Benzoate, 0.5% Silicone Oil, Deionized water to make up the balance;

[0056] Preparation method: The same as Preparation Example 3.

[0057] Preparation Example 7: 16% Sulfiflumin·Fenbutatin Oxide Emulsifiable Concentrate (15:1)

[0058] Formulation composition: 15% Sulfiflumin, 1% Fenbutatin Oxide, 15% N-Methylpyrrolidone, 12% Fatty Alcohol Polyoxyethylene Ether, 2% Succinate Sulfonate, 8% DMF, Methyl Oleate to make up the balance;

[0059] Preparation method: The same as Preparation Example 4.

[0060] Example 1: Indoor Bioassay Activity Determination

[0061] Test basis: The test refers to NY / T1154-12-2008 "Pesticide Indoor Bioassay Test Guidelines Insecticides Part 12: Slide Immersion Method for Tetranychus urticae".

[0062] Biological test materials: Panonychus ulmi (Koch).

[0063] Test agents: Sulfiflumin technical, Azocyclotin technical, Fenbutatin Oxide technical.

[0064] Test preparation: Select female adult mites with consistent physiological states reared indoors. Cut double-sided tape into 2 cm lengths and stick one end to a glass slide. Then select healthy mites and stick their backs to the double-sided tape, 30 mites per slide, and place them in a container lined with a wet sponge, cover with a lid, and place at (25±1)°C. After 2 h, conduct microscopic examination, remove dead and injured individuals, and make up to 30 mites per slide.

[0065] Pharmaceutical preparation: The original drug is formulated into a stock solution with an organic solvent, and then into 5 series of mass concentrations with a 0.1% Tween-80 aqueous solution.

[0066] Pharmaceutical treatment: The glass slides are immersed in the medicinal solution, gently shaken for 5 s and then taken out, the excess medicinal solution is blotted with absorbent paper, placed in a white disk lined with a moist sponge, and covered with a plastic film with good light transmittance.

[0067] Each treatment is repeated 4 times, and a treatment without the drug (containing all organic solvents and emulsifiers) is set as a blank control.

[0068] Rearing and observation: The containers containing the treated test insects are placed under the conditions of a temperature of (25 ± 1) °C and a photoperiod of L:D = (16:8) h for rearing and observation.

[0069] Inspection: The mortality of the test insects is inspected 48 h after treatment, and the total number of insects and the number of dead insects are recorded separately.

[0070] Calculation method:

[0071] According to the investigation data, the mortality of each treatment is calculated. It is calculated according to the following formula:

[0072]

[0073] In the formula:

[0074] P - Mortality rate, in percentage (%);

[0075] K - Represents the number of dead insects, in number;

[0076] N - Represents the total number of treated insects, in number.

[0077]

[0078] In the formula:

[0079] P1 - Corrected mortality rate, in percentage (%);

[0080] P t - Treatment mortality rate, in percentage (%);

[0081] P0 - Mortality rate of the blank control, in percentage (%).

[0082] If the mortality rate of the control < 5%, no correction is needed; if the mortality rate of the control is between 5% and 20%, correction should be made according to formula (2); if the mortality rate of the control > 20%, the experiment needs to be redone.

[0083] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formulas (3), (4), and (5):

[0084]

[0085] In the formula:

[0086] ATI—the measured virulence index of the mixture;

[0087] S—the LC 50 of the standard acaricide, with the unit of milligram per liter (mg / L);

[0088] M—the LC 50 of the mixture, with the unit of milligram per liter (mg / L).

[0089] TTI = TI A ×P A +TI B ×P B

[0090] In the formula:

[0091] TTI—the theoretical virulence index of the mixture;

[0092] TI A —the virulence index of pesticide A;

[0093] P A —the percentage content of pesticide A in the mixture, with the unit of percentage (%);

[0094] TI B —the virulence index of pesticide B;

[0095] P B —the percentage content of pesticide B in the mixture, with the unit of percentage (%).

[0096]

[0097] In the formula:

[0098] CTC—coefficient of co-toxicity;

[0099] ATI—the measured virulence index of the mixture;

[0100] TTI—the theoretical virulence index of the mixture.

[0101] When the co-toxicity coefficient CTC of the compounding is ≥120, it shows a synergistic effect; when CTC ≤80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect. The results of the indoor virulence test are shown in the following table:

[0102] Table 1 Test results of the indoor biological activities of sulfiflumin and azocyclotin against Panonychus ulmi

[0103] Test agents <![CDATA[LC 50 (mg / L)]]> ATI TTI Coefficient of co-toxicity (CTC) Sulfiflumin (A) 1.2686 100.000 / / Azocyclotin (B) 10.3170 12.296 / / A:B = 1:40 7.0003 18.122 14.435 125.540 A:B = 1:30 6.3195 20.074 15.125 132.720 A:B = 1:20 5.0313 25.214 16.473 153.067 A:B = 1:10 3.5475 35.760 20.269 176.427 A:B = 1:5 2.5860 49.056 26.914 182.274 A:B = 5:1 0.8911 142.363 85.383 166.736 A:B = 10:1 0.9311 136.247 92.027 148.052 A:B = 20:1 0.9852 128.766 95.824 134.378 A:B = 30:1 1.0323 122.891 97.171 126.469 A:B = 40:1 1.1936 106.284 97.861 108.607 A:B = 50:1 1.2837 98.824 98.280 100.553

[0104] Table 2 Results of indoor bioactivity determination test of sulfiflumin and fenbutatin oxide against Panonychus ulmi

[0105]

[0106]

[0107] From the indoor test results in Table 1 - 2, it can be seen that the pesticide composition of the present invention has good control effect against Panonychus ulmi. When sulfiflumin is compounded with azocyclotin, when the mass ratio of the two is 1:40 - 30:1, it shows a synergistic effect on Panonychus ulmi, and when the mass ratio is 40:1 - 50:1, it shows an additive effect on Panonychus ulmi; when sulfiflumin is compounded with fenbutatin oxide, when the mass ratio of the two is 1:45 - 35:1, the co - toxicity coefficient against Panonychus ulmi is greater than 120, showing a synergistic effect, while when the mass ratio is 1:55, 45:1 - 55:1, its co - toxicity coefficient is less than 120, showing an additive effect.

[0108] Example 2: Field efficacy test against Panonychus ulmi

[0109] Test site: Apple orchard in Wangjiazhuang Village, Zhaoyuan City, Shandong Province. The soil fertility of the test site is medium to above - medium, and the irrigation conditions are good, meeting the local scientific agricultural practices.

[0110] Test crop: Red Fuji.

[0111] Test target: Panonychus ulmi, Tetranychus viennensis.

[0112] Arrangement of test plots: Each test plot consists of 2 fruit trees, and each treatment is repeated 4 times.

[0113] Application time: Pesticide application for control is carried out when the average number of mites per leaf reaches 7 - 11. When applying pesticides, a knapsack manual sprayer is used to spray the entire apple plant until the apple leaves are wet with pesticide but do not drip.

[0114] Test investigation: The mite population base is investigated before pesticide application, and the number of live mites is investigated on the 1st, 5th, and 10th days after pesticide application. All 2 trees in each plot are investigated. For each tree, 5 leaves are marked at 5 positions of east, south, west, north, and middle, and all live mites are investigated and recorded with a hand - held magnifying glass. At the same time, the tree body reaction and the impact on natural enemies are investigated.

[0115] Method for calculating efficacy:

[0116]

[0117]

[0118] Results and analysis of field efficacy test:

[0119] Table 3 Results of Field Efficacy Test against Apple Tetranychus

[0120]

[0121] It can be seen from the above field efficacy test results that the pesticide composition of the present invention has excellent control effects against Apple Tetranychus, and has good quick-acting properties compared with the single-agent control. And within the test dose range, no phytotoxicity was found on apple plants, and it is relatively safe for natural enemies, so it can be applied and promoted in production.

[0122] Example 3: Field Efficacy Test against Citrus Red Spider

[0123] Test Site: The test was carried out in a Satsuma mandarin orchard in Pingling Village, Yongfu Town, Yongfu County, Guangxi. The soil fertilizer, management conditions, etc. of the test site were basically uniform.

[0124] Test Plot Setting: A total of 5 pesticide treatments and 1 clear water control treatment were set in the test. Each plot was arranged in a random block design. Each treatment plot had 2 citrus trees, and each treatment was repeated 4 times. There were 2 Satsuma mandarins in each plot, with a total of 48 test plots, and a protection row was set around the plots.

[0125] Pesticide Application Time and Method: Pesticide application was carried out on September 16, 2023. The conventional crown spraying method was used during pesticide application. There was no rainfall on the day of pesticide application and within 2 days after application, and no adverse weather factors affecting the test results occurred.

[0126] Investigation Time and Method: The initial population density of pests was investigated before pesticide application, and the number of surviving mites was investigated 3 days, 10 days, and 20 days after pesticide application, for a total of 4 investigations. In each plot, 1 tree tip was marked at the east, south, west, north, and middle of each tree. A total of 50 leaves on each plot were investigated for the number of active mites, and the number of live mites was counted.

[0127] Calculation Method of Efficacy:

[0128]

[0129]

[0130] Results and Analysis of Field Efficacy Test:

[0131] Table 4: Field Efficacy Test against Citrus Red Spider

[0132]

[0133]

[0134] The investigation results 3 days after application showed that the control effects of 21% sulfiflumin·azocyclotin water dispersible granules (6:1) and 16% sulfiflumin·fenbutatin oxide emulsifiable concentrate (15:1) were 86.77% and 85.61% respectively; 20 days after application, the control effect of the pesticide composition of the present invention against Panonychus citri McGregor was significantly higher than that of each single agent control, and the long-lasting period was longer. Within the tested concentration range, no obvious phytotoxicity of the pesticide composition of the present invention to citrus leaves, young shoots and fruits was observed after application.

[0135] From the results of indoor and field efficacy tests, it can be seen that the pesticide composition of the present invention has excellent control effect against Panonychus ulmi or Panonychus citri McGregor, effectively reducing the damage of harmful mites to crops and inhibiting the outbreak of harmful mite populations.

[0136] Although the present invention has been described in detail with general descriptions and specific embodiments above, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A pesticide composition, characterized in that, The active ingredients of the pesticide composition comprise active ingredient A and active ingredient B. The active ingredient A is sulfiflumin, and the active ingredient B is azocyclotin. The mass ratio of active ingredient A to active ingredient B in the pesticide composition is 1:40 to 30:

1.

2. The pesticidal composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:30 to 20:

1.

3. The pesticide composition according to claim 1, characterized in that, In addition to the active ingredients, the pesticide composition further comprises agriculturally acceptable auxiliary ingredients, which are fillers and / or surfactants.

4. The pesticide composition according to claim 1, characterized in that, The formulation form of the pesticide composition is a solution, suspension concentrate, emulsifiable concentrate, emulsion in water, microcapsule suspension, microemulsion, suspoemulsion, dispersible oil suspension, granule, water dispersible granule or wettable powder.

5. The pesticide composition according to claim 4, characterized in that, The formulation form is a suspension concentrate, emulsifiable concentrate, water dispersible granule or wettable powder.

6. Use of the pesticide composition according to any one of claims 1-5 for controlling citrus red mites and apple red mites.

7. The use according to claim 6, wherein The citrus red mite is Panonychus citri, and the apple red mite is Panonychus ulmi or Tetranychus viennensis.

8. The use according to claim 6, characterized in that, Apply the pesticide composition to the pest mites and the plants on which they grow or the environment in which they grow.

Images