A method for continuous high-voltage pulsed electric field treatment of functional active ingredient fortified extraction of berries

Abstract

The application discloses a kind of continuous high-voltage pulse electric field processing berry functional active ingredient reinforced extraction method.The method comprises the following steps: after the cooling device is cooled to reach the set temperature by the processing chamber device, set high-voltage pulse power output parameter, high-voltage pulse power generates bipolar symmetric square wave, set pulse electric field parameter, the distance of two titanium alloy electrodes is 100mm, the pretreated berry material is transported to the processing chamber body, and the high-voltage pulse electric field processing is carried out on the berry material, to obtain the berry extract containing active ingredient.The method proposed in the application uses continuous round square corrosion-resistant pulse electric field processing chamber, the overall structure is convenient to assemble and process, the processing chamber can be increased according to actual needs, and synchronous cooling can be realized at the same time, the low-temperature extraction and active protection of functional active ingredient are realized while the electrode is protected.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of food mechanical processing and food process optimization application, in particular to a method for continuous high-voltage pulse electric field treatment of functional active ingredient fortified extraction of berry. BACKGROUND

[0002] High-voltage pulse electric field technology (HPEF) is based on the principle of applying a specific pulse high voltage between two electrodes, using the high potential difference between the electrodes to generate a high-intensity electric field (for example, parallel plate electric field: electric field strength E = U / d, where E is in kV / cm, U is high voltage in kV, and d is the distance between parallel plate electrodes in cm). Under the action of a certain intensity and direction of high-voltage electric field, the molecules of the material are polarized and arranged in a certain direction due to the asymmetry of the individual components of the material. A potential difference is formed on both sides of the cell membrane (used to balance or offset the external applied electric field pressure). When the external electric field strength exceeds the tolerance of the cell membrane, the particles in the cytoplasm are pulled by the electric field force to form an internal electric field that squeezes the cell membrane. The permeability of the cell membrane on both sides increases, the membrane strength is weakened, the tissue structure is shaken and destroyed, and the cytoplasm in the cell membrane flows out, thereby achieving the purpose of increasing the content of certain components in the material. High-voltage pulse instantaneous destruction has strong power, low average power, and little heat production, and can be quickly and efficiently applied to functional active ingredient extraction, such as the extraction of polyphenols and flavonoids from grape skin and residue, and will not affect the structure of the extract and the flavor of the material. It has a wide range of applications and has been favored by many research fields.

[0003] However, due to the complexity of food material characteristics, the difference between different food material characteristics and processing methods, and the imperfection of related processing modes and product development operation procedures, and due to the large scale of food processing, most of the materials to be processed are slurry materials, which have fixed resistance and high conductivity. Scale processing is carried out through pumping and other methods. Under the action of high-voltage instantaneous electric field, the conductivity changes greatly, and the pulse electric field discharge processing model is different from the traditional pure resistance discharge system. The material processing process involves resistance-capacitance discharge, which greatly increases the difficulty of material processing. At the same time, continuous pulse electric field treatment requires higher stability and reliability of the power supply, uniformity of material processing in the pulse electric field treatment chamber, and corrosion resistance of the electrode.

[0004] The current high-voltage pulsed electric field equipment is mainly laboratory-scale equipment. Chinese patent 202022239622.3 discloses a high-voltage pulsed electric field sterilization device with multiple needle-shaped electrodes and a liftable treatment chamber. The sample is placed on the lifting device, and the height is adjusted for the reduction of bacteria in meat and other materials. Chinese patent 202120190221.5 discloses an ultrasonic-assisted high-voltage pulsed electric field extraction tank. A static high-voltage pulsed electric field is added to the top of the cylindrical tank, and an ultrasonic wave is installed at the bottom. The existing patent CN108452006A discloses a method for extracting plant polyphenols by combining pulsed electric field, aqueous two-phase system, and column chromatography. The pulsed electric field static treatment chamber is used for 20-30 pulse discharge treatment to extract the active ingredients of plants. Chinese patent 202122150584.9 discloses an ultrasonic-assisted high-voltage pulsed electric field extraction device for bergamot fruit functional ingredients. The device uses ultrasonic waves to assist the pulsed electric field to strengthen the extraction of bergamot fruit functional ingredients. Chinese patent 201710929691.7 discloses a device and method for extracting traditional Chinese medicine ingredients using electroporation technology. The treatment chamber is a coaxial treatment chamber containing a high-voltage electrode array and a grounding electrode array. The electrode distance is controlled between 0.5-5 cm, which can meet the extraction and processing requirements of small particles or liquid materials.

[0005] In summary, the existing high-voltage pulsed electric field extraction equipment has the following problems:

[0006] 1. The current high-voltage pulsed electric field extraction equipment is mostly laboratory-scale equipment or static treatment. The scale is small, and most of the extraction methods are assisted by ultrasonic waves. The industrial processing equipment for slurry materials is lacking, and it cannot meet the needs of industrial extraction and processing.

[0007] 2. The current continuous high-voltage pulsed electric field extraction equipment is mainly of the common field type and coaxial type. The flat plate type processing device is less common due to its complexity, and the temperature rise control and electrode corrosion during high-voltage pulsed electric field processing are ignored. The higher the temperature, the faster the electrode corrosion rate. In severe cases, the electrode dissolves and contaminates the material, which cannot guarantee the safety of processing.

[0008] 3. The existing high-voltage pulsed electric field processing material temperature rise control uses pre-cooling or post-cooling treatment. The material is quickly heated through the electrodes and then condensed through a long pipeline. The active ingredients are not effectively protected during the process. Summary of the invention:

[0009] In order to overcome the above problems of the prior art, the present application provides a method for continuous high-voltage pulse electric field treatment of functional active ingredient reinforced extraction of berries, which uses a high-voltage pulse square wave treatment power supply as a treatment power supply (power supply parameters: 0-±25kV, symmetric pulse square wave, 1-1000Hz adjustable, 1-20μs adjustable, 1000A protection current), and is equipped with a peristaltic pump for conveying materials and a synchronous cooling treatment chamber system, which cools the materials and condenses the electrodes to protect the electrodes from corrosion, greatly reduces the corrosion of the electrodes, prolongs the service life of the electrodes, reduces the risk of electrode dissolution contaminating the materials, and improves the safety of the processed materials, and the maximum treatment capacity can reach 3.0t / h, which can meet the industrial treatment of various pumpable materials.

[0010] The present application aims to provide a method for continuous high-voltage pulse electric field treatment of functional active ingredient reinforced extraction of berries, which is achieved by connecting an extraction device and a material control system through a high-voltage pulse electric field, wherein the high-voltage pulse electric field extraction device and the material control system comprise a treatment chamber device and a cooling device, the treatment chamber device comprises titanium alloy electrodes, stainless steel sealing plates arranged on both sides, and an insulating support arranged in the middle, the titanium alloy electrodes are symmetrically embedded in the insulating support, the stainless steel sealing plates are fixed to the outer edge of the insulating support, the stainless steel sealing plates, the titanium alloy electrodes and the insulating support form a sandwich cavity as a whole, the bottom of the sandwich cavity is connected with the refrigerant inlet and the refrigerant outlet of the cooling device, the refrigerant enters the sandwich cavity through the refrigerant inlet to cool the titanium alloy electrodes, and then the refrigerant is discharged through the refrigerant outlet, and the output end of the high-voltage pulse power supply is connected with the titanium alloy electrodes to generate a high-voltage pulse electric field; the method comprises the following steps: after the treatment chamber device is cooled to a set temperature through the cooling device, the output parameters of the high-voltage pulse power supply are set, the high-voltage pulse power supply generates a bipolar symmetric square wave, the pulse electric field parameters are set, the distance between the two titanium alloy electrodes is 100mm, the pretreated berry material is conveyed to the treatment chamber device, the berry material is treated by a high-voltage pulse electric field, the feeding temperature and the discharging temperature of the berry material are the same, and the berry extraction liquid containing active ingredients is obtained.

[0011] The technical principle of the method is that when the berry material passes through the high-voltage pulse electric field treatment chamber, an internal electric field is quickly formed inside the material to cope with the external electric field pressure. Since the applied external pulse high voltage is a symmetrical bipolar pulse, the polarity of the external pulse voltage changes, such as when the applied voltage is a reverse voltage, the instantaneous potential difference applied to the berries quickly changes to double the voltage intensity. When the intensity exceeds the intensity that the berry cells can withstand, the berry cells quickly rupture, achieving the dissolution of the functional components in the cells. Since the material processing time is short (the total processing time is less than 5 ms), the heat generated by the high voltage is small, and since the cavity of the treatment chamber is cooled by circulating cooling liquid, the temperature rise is reduced, and the protection of heat-sensitive components is achieved. By pre-cooling the electrodes, the temperature difference between the cooled electrodes and the material is improved, and the heat transfer efficiency is improved. The material delivery pump flow is controlled to keep the material at a constant temperature before and after passing through the cavity, that is, the temperature of the material entering and leaving the cavity does not change. The work done by the polar molecular directional movement electric field force caused by the internal rupture of the material in a short time and the heat effect caused by a small part of the high-voltage pulse current are completely balanced by the cooling system. The high-voltage pulse electric field electrode material is Ti-0.2Pd corrosion-resistant high-hardness titanium alloy. The bipolar electrode is cooled by cooling liquid and always kept at 0 DEG C or below. The titanium alloy has strong corrosion resistance at low temperature, and a thin layer of ice forms on the surface of the titanium alloy electrode at low temperature, embedding the titanium alloy electrode and preventing direct contact between the titanium electrode and the loaded material, thereby avoiding electrode corrosion and improving the protection of the titanium alloy electrode. The processing pulse width is always less than or equal to 10 microseconds during the material processing process. The narrow pulse width is controlled at a frequency of 200-500 Hz, reducing the energy density acting on the titanium alloy electrode per unit time, and the titanium alloy electrode does not reach the condition of electron escape, reducing electrode corrosion. The titanium alloy electrode is a smooth parallel plate electrode, and the edge is embedded in the insulating material polytetrafluoroethylene to avoid edge high-voltage discharge. The parallel plate electrode has a uniform electric field distribution, reducing the local breakdown effect of high-conductivity materials under high voltage in the treatment chamber, reducing the current effect, and correspondingly improving the electric field effect. The strengthening of the electric field effect correspondingly expands the high-voltage electric field induced material tissue rupture effect. At the same time, the use of bipolar symmetrical square wave electrodes binds the flow of two-way local current-carrying electrons, reduces the overflow of high-energy active electrons, and realizes pollution-free processing of the slurry.

[0012] Preferably, the titanium alloy electrode includes a first titanium alloy electrode and a second titanium alloy electrode symmetrically embedded in an insulating support, the titanium alloy electrode material is Ti-0.2Pd corrosion-resistant high-hardness titanium alloy, the stainless steel sealing plate includes a first stainless steel sealing plate and a second stainless steel sealing plate symmetrically arranged on both sides of the processing chamber device, the first stainless steel sealing plate, the first titanium alloy electrode and the insulating support form a first sandwich cavity, the second stainless steel sealing plate, the second titanium alloy electrode and the insulating support form a second sandwich cavity, the first sandwich cavity and the second sandwich cavity are communicated through a connecting hole, the connecting hole is on the insulating support, the first sandwich cavity and the second sandwich cavity are connected, the bottom of the first sandwich cavity is connected with a refrigerant inlet, and the bottom of the second sandwich cavity is connected with a refrigerant outlet; during use, the refrigerant enters and exits through the connecting hole on the insulating support to control the temperature.

[0013] Preferably, the first titanium alloy electrode is welded with a first threaded screw rod in the middle, the first threaded screw rod is connected with the first stainless steel sealing plate through threads, and is fixed through a first inner locking nut; the second titanium alloy electrode is welded with a second threaded screw rod in the middle, the second threaded screw rod is connected with the second stainless steel sealing plate through threads, and is fixed through a second inner locking nut.

[0014] Preferably, the first stainless steel sealing plate is provided with a first stainless steel sealing plate screw hole, the first stainless steel sealing plate is fixed on the outer edge of the insulating support through a first sealing insulating screw, the second stainless steel sealing plate is provided with a second stainless steel sealing plate screw hole, and the second stainless steel sealing plate is fixed on the outer edge of the insulating support through a second sealing insulating screw.

[0015] Preferably, the berry material has an electrical conductivity of 1800-4000 μS / cm.

[0016] Further preferably, the berry material is grape or mulberry. The berry material is delivered by a material pump, the pump flow parameter is 0.5-3.0 t / h, and the material pump can be a screw pump or a self-priming pump.

[0017] Preferably, the refrigerant temperature in the cooling device is set to-5-8℃, the refrigerant liquid flow rate is 300-500 L / h, the refrigerant circulates for 5-10 min to reach a set temperature of 0℃ or below, and the electrode surface is covered with a thin layer of ice crystal.

[0018] Preferably, the berry material feeding temperature is 15-30℃, the berry material discharging temperature is adjusted by adjusting the refrigerant liquid flow rate and temperature of the electrode and the material feeding speed, so that the material discharging temperature is consistent with the feeding temperature, that is, there is no temperature rise during the material processing.

[0019] Preferably, the pulse electric field parameters are: pulse electric field intensity 0.6-2.2 kV / cm, frequency 200-500 Hz, pulse width 6-10 mu s.

[0020] Preferably, the processing chamber device is a round-to-square structure, i.e. the pipe connecting the material is in a circular structure, and the structure between the two titanium alloy electrode plates is in a square structure, forming a parallel plate structure, and a groove for installing a sealing ring is arranged between the titanium alloy electrode and the insulating support.

[0021] During production, the pulp of the material to be treated (grapes, mulberries, etc. are crushed and pressed into pulp) is placed in the container to be treated, and the material to be treated can be preheated or pre-cooled in the pre-treatment tank. The relevant parameters such as the conductivity and temperature of the material to be treated are measured, and the appropriate conductivity is less than 4000 mu S / cm. The temperature of the material to be treated is adjusted according to the actual production needs. The material to be treated tank, the material conveying pump, and the processing chamber device are connected through pipes. The cooling water inlet and outlet of the processing chamber device are connected with the cooling refrigeration unit. The processing chamber electrode is connected with the high-voltage pulse power output end. All equipment is safely and effectively grounded.

[0022] During processing and production, the cooling device is first turned on, the refrigerant temperature is set to -5-8 DEG C, the refrigerant liquid flow rate is 300-500 L / h, the pump flow parameter is 0.5-3.0 t / h, the pump can be a screw pump or a self-priming pump, and the pump flow parameter can be adjusted through a frequency converter. After the refrigerant circulates for 5-10 min, a thin layer of small ice crystals covers the surface of the titanium alloy electrode in the processing chamber. The material conveying pump is started, the high-voltage pulse parameters are set, the high-voltage pulse power output is started, the fiber is used to measure the feeding temperature and discharging temperature of the material into and out of the processing chamber, the material conveying pump conveying speed is adjusted according to the feeding temperature and discharging temperature of the material, the material flow rate is increased when the temperature is increased, and the pump flow parameter is adjusted in coordination with the feeding temperature and discharging temperature of the material to ensure that the feeding temperature and discharging temperature of the material are consistent.

[0023] Preferably, the active ingredient in the berry extract containing the active ingredient is polyphenol.

[0024] Compared with the prior art, the present application has the following advantages:

[0025] 1. The continuous high-voltage pulse electric field processing method for functional active ingredient of berry can meet the industrialized extraction processing; the processing chamber used in the method is a parallel plate type, which can form a relatively uniform electric field, and can generate a larger high-voltage pulse without local breakdown current. The high-voltage pulse instantaneous power is high, which can reach megawatt level, but the average power is low (kilowatt level), the effective processing time is short, and there is basically no temperature rise and no thermal effect.

[0026] 2. The processing chamber of the extraction method is composed of corrosion-resistant titanium alloy electrode plate and high-insulation material polytetrafluoroethylene, the titanium alloy electrode is embedded with polytetrafluoroethylene insulation material at the edge, avoiding the edge discharge breakdown effect under high voltage, and at the same time combining with synchronous cooling low-temperature electrode protection, combining with bipolar symmetric square wave to reduce corrosion, and improving the service life of titanium alloy electrode.

[0027] 3. The high-voltage pulsed electric field processing cavity of the extraction method can be quickly connected according to the specific process. It can meet the electric field processing of various materials, especially in the extraction of berries, and has strong operability and wide use range. The parameter adjustable range is wide, and the cleaning and disassembly are convenient. The size of the related material external interface is a standard size, and multiple processing cavities can be used in series according to the actual production needs. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 It is a high-voltage pulsed electric field connection extraction device and material control system structure schematic diagram;

[0029] Figure 2 It is a high-voltage pulsed electric field processing chamber device structure schematic diagram;

[0030] BRIEF DESCRIPTION OF DRAWINGS: 1, high-voltage pulse power supply; 2, material conveying pump; 3, processing chamber device; 3-1, first stainless steel sealing plate screw hole; 3-2, first stainless steel sealing plate; 3-3, first outer locking nut; 3-4, first titanium alloy electrode; 3-5, first polytetrafluoroethylene support threaded hole; 3-6, pipeline connection threaded hole; 3-7, communication hole; 3-8, first inner electrode groove; 3-9, contact processing area; 3-10, second threaded screw; 3-11, second inner locking nut; 3-12, refrigerant inlet; 3-13, refrigerant outlet; 4, cooling device. DETAILED DESCRIPTION

[0031] The following examples are further illustrations of the application and are not intended to limit the same.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the protection scope of the application. Unless otherwise specified, the experimental materials and reagents in this paper are commonly used in the art.

[0033] As Figure 1 and 2As shown, a method for continuous high-voltage pulse electric field treatment of functional active ingredient extraction of berries, which is realized by connecting the extraction device and the material control system through high-voltage pulse electric field, and the high-voltage pulse electric field connecting the extraction device and the material control system includes a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3 and a cooling device 4. The high-voltage pulse power supply 1 provides power for the high-voltage pulse electric field in the treatment chamber device 3, and the berry material is sent into the treatment chamber device 3 for treatment through the material conveying pump 2. The treatment chamber device includes titanium alloy electrodes, stainless steel sealing plates arranged on both sides and an insulating support arranged in the middle. The titanium alloy electrodes are symmetrically embedded in the insulating support, and the stainless steel sealing plates are fixed to the outer edge of the insulating support. The stainless steel sealing plate, titanium alloy electrode and insulating support form a sandwich cavity as a whole. The bottom of the sandwich cavity is connected with the coolant inlet 3-12 and the coolant outlet 3-13 of the cooling device. The coolant enters the sandwich cavity through the coolant inlet 3-12 to cool the titanium alloy electrode, and then the coolant is discharged through the coolant outlet 3-13. The output end of the high-voltage pulse power supply 1 is connected with the titanium alloy electrode to generate a high-voltage pulse electric field.

[0034] The titanium alloy electrode includes a first titanium alloy electrode 3-4 and a second titanium alloy electrode symmetrically embedded in the insulating support. The stainless steel sealing plate includes a first stainless steel sealing plate 3-2 and a second stainless steel sealing plate symmetrically arranged on both sides of the treatment chamber device. The first stainless steel sealing plate 3-2, the first titanium alloy electrode 3-4 and the insulating support form a first sandwich cavity as a whole. The second stainless steel sealing plate, the second titanium alloy electrode and the insulating support form a second sandwich cavity as a whole. The first sandwich cavity and the second sandwich cavity are connected through a communication hole 3-7. The bottom of the first sandwich cavity is connected with the coolant inlet 3-12, and the bottom of the second sandwich cavity is connected with the coolant outlet 3-13. A first threaded screw rod is welded in the middle of the first titanium alloy electrode. The first threaded screw rod is connected with the first stainless steel sealing plate 3-2 through threads and is fixed through a first inside locking nut. A second threaded screw rod 3-10 is welded in the middle of the second titanium alloy electrode. The second threaded screw rod 3-10 is connected with the second stainless steel sealing plate through threads and is fixed through a second inside locking nut 3-11. The first stainless steel sealing plate 3-2 is provided with a first stainless steel sealing plate screw hole 3-1. The first stainless steel sealing plate 3-2 is fixed to the outer edge of the insulating support through a first sealing insulation screw. The second stainless steel sealing plate is provided with a second stainless steel sealing plate screw hole. The second stainless steel sealing plate is fixed to the outer edge of the insulating support through a second sealing insulation screw.

[0035] The high-voltage pulse power supply 1 can generate positive and negative pulse voltages of 0-25kV, with a maximum current resistance of 1000A. The high-voltage pulse waveform rising edge is <500ns, the pulse waveform falling edge is <500ns, the pulse width output is 1-20μs, and the frequency output is 1-1000Hz.

[0036] In the following examples, the two output terminals of the high-voltage pulse power supply are directly connected to the first outer locking nut 3-3 of the first titanium alloy electrode 3-4 and the second outer locking nut of the second titanium alloy electrode of the processing chamber device 3 through wires; the processing chamber device 3 has a round-to-square structure, and the overall support insulating material is polytetrafluoroethylene (the outer size is 200mm×200mm×200mm). The upper and lower of the insulating support are through-pipe connection threaded holes 3-6, which are convenient for connecting with external pipes, and the inner hole size is 100mm in diameter.

[0037] The left and right sides of the insulating support are embedded with titanium alloy electrodes (inner electrodes, the left and right sides are symmetrical structures). The first titanium alloy electrode 3-4 has a first inner electrode groove 3-8 between the first titanium alloy electrode 3-4 and the insulating support, and a sealing ring is installed in the middle of the first inner electrode groove 3-8. The size of the first titanium alloy electrode 3-4 is 80mm×80mm×5mm (length×width×thickness), and the contact area between the first titanium alloy electrode 3-4 and the polytetrafluoroethylene insulating support is 50mm×50mm. The effective contact processing area 3-9 of the left and right parallel plate titanium alloy electrodes is 50mm×50mm, and the titanium alloy electrodes on the left and right sides (i.e. the first titanium alloy electrode and the second titanium alloy electrode) have a distance of 100mm. The second titanium alloy electrode has a second inner electrode groove between the second titanium alloy electrode and the insulating support, and a sealing ring is installed in the middle of the second inner electrode groove. The size of the second titanium alloy electrode 3-4 is 80mm×80mm×5mm (length×width×thickness), and the contact area between the second titanium alloy electrode and the polytetrafluoroethylene insulating support is 50mm×50mm. The distance between the titanium alloy electrodes on the left and right sides (i.e. the first titanium alloy electrode and the second titanium alloy electrode) is 100mm.

[0038] The first threaded screw is welded in the middle of the first titanium alloy electrode 3-4, the first threaded screw is connected with the first stainless steel sealing plate 3-2 through threads (the size of the stainless steel sealing plate is 200mm×200mm×5mm, length×width×thickness), and is fixed through the first inner locking nut. The second threaded screw 3-10 is welded in the middle of the second titanium alloy electrode, the second threaded screw 3-10 is connected with the second stainless steel sealing plate through threads (the size of the stainless steel sealing plate is 200mm×200mm×5mm, length×width×thickness), and is fixed through the second inner locking nut 3-11.

[0039] The first stainless steel sealing plate 3-2 has first stainless steel sealing plate screw holes 3-1 and first polytetrafluoroethylene support threaded holes 3-5, and the first stainless steel sealing plate 3-2 is fixed to the outer edge of the overall support through sealing insulation screws (PEEK screws), the contact thickness between the outer edge of the support and the first stainless steel sealing plate 3-2 is 20 mm, the insulation support has grooves and first polytetrafluoroethylene support threaded holes 3-5, sealing rings are additionally installed between the grooves, the first stainless steel sealing plate 3-2 has first stainless steel sealing plate screw holes 3-1, and the first stainless steel sealing plate 3-2 is fixed to the insulation support through insulation screws (PEEK screws), after the first stainless steel sealing plate 3-2 is fixed to the second titanium alloy electrode through screwing, the left sandwich cavity is formed with the support as a whole. The second stainless steel sealing plate has second stainless steel sealing plate screw holes and second polytetrafluoroethylene support threaded holes, and the second stainless steel sealing plate is fixed to the outer edge of the overall support through sealing insulation screws (PEEK screws), the contact thickness between the outer edge of the support and the second stainless steel sealing plate is 20 mm, the insulation support has grooves and second polytetrafluoroethylene support threaded holes, sealing rings are additionally installed between the grooves, the second stainless steel sealing plate has second stainless steel sealing plate screw holes, and the second stainless steel sealing plate is fixed to the insulation support through insulation screws (PEEK screws), after the second stainless steel sealing plate is fixed to the second titanium alloy electrode through screwing, the right sandwich cavity is formed with the support as a whole. The overall structure of the processing chamber device forms a left sandwich cavity and a right sandwich cavity, the left sandwich cavity and the right sandwich cavity are connected through a communication hole 3-7, the hole has a direct through structure, the left sandwich cavity is connected with the refrigerant inlet 3-12 of the cooling device at the bottom, the right sandwich cavity is connected with the refrigerant outlet 3-13 of the cooling device at the bottom, and the cooling device 4 is connected, synchronous circulation cooling is realized, and the specific processing chamber device is shown in Figure 2 .

[0040] Example 1

[0041] As shown in Figure 1 , a method for continuously processing high-voltage pulse electric field to strengthen and extract functional active ingredients of berries, the implementation of the method needs a high-voltage pulse power supply 1, a material conveying pump 2, a processing chamber device 3, a cooling device 4 and other related devices. The various system devices are connected through pipelines, and the processing chamber device 3 is connected with the high-voltage pulse power supply 1, the material conveying pump 2 and the cooling device 4. Figure 1The connections were made in the order shown. 200 kg of Muscat grapes were purchased from the market. After destemming and removing leaves, the grapes were crushed using a screw press. The initial conductivity of the grape pulp was measured to be 1800 μS / cm using a conductivity meter. The initial temperature of the grape pulp was 20℃. The output parameters of the high-voltage pulse power supply were set as follows: pulse electric field strength 0.6 kV / cm, frequency 500 Hz, pulse width 10 μs, bipolar symmetrical square wave pulse. After setting the parameters, the system was ready to start. The grape pulp was transported to the processing chamber device 3 via a pipeline by material conveying pump 2. The feeding method was bottom feeding and top discharging. The cooling device inlet of the processing chamber was connected to a refrigeration unit. The refrigeration unit used an 8% alcohol aqueous solution as the cooling circulating fluid. The cooling liquid temperature was -5℃, the cooling liquid flow rate was 300 L / h, and the pump flow rate parameter was 0.5 t / h. After connecting the processing chamber to the refrigeration system, first turn on the cooling device and run the refrigeration unit's piping for about 5 minutes. Once small ice crystals appear on the surface of the titanium alloy inner electrode in the processing chamber or the coolant temperature in the cooling circulation system reaches the set stable temperature, start the material pump to deliver the grape pulp. After the grape pulp passes through the processing chamber, start the high-voltage pulse power supply output. Use a fiber optic thermometer to measure the grape pulp inlet temperature; it should be 20℃, and the outlet temperature should also be 20℃, meeting the overall stable condition. No pump flow rate adjustment is needed. After processing, turn off the high-voltage pulse power supply output, stop the material delivery pump, and shut down the cooling system. The polyphenol content of the grape pulp obtained after processing was measured to be 1208 mg / L, while the polyphenol content without high-voltage pulse electric field treatment was 1062 mg / L.

[0042] Comparative Example 1 (the application effect is not obvious at electric field strengths below 0.6 kV / cm)

[0043] like Figure 1 As shown, a method for enhanced extraction of functional active ingredients from berries using a continuous high-voltage pulsed electric field is described. This method requires the auxiliary support of a high-voltage pulsed power supply 1, a material conveying pump 2, a processing chamber 3, and a cooling device 4. The various system devices are connected via... Figure 1The connection and fixation are carried out in the shown order, 200 kg of rose grape is purchased on the market, after the grape stems and leaves are removed, the grape is crushed by a screw press, the initial conductivity of the grape pulp is measured by a conductivity meter, the initial temperature of the grape pulp material is 20°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 0.5 kV / cm, the frequency is 500 Hz, the pulse width is 10 μs, the bipolar symmetric square wave pulse is set, and the parameters are started after being set. The grape pulp is conveyed to the treatment chamber device 3 through the pipeline by the material conveying pump 2, the feeding mode is feeding from the bottom and discharging from the top. The cooling device of the treatment chamber is connected to the refrigeration unit, the refrigeration unit uses 8% alcohol water solution as the cooling circulating liquid, the temperature of the cooling liquid is -5°C, the flow rate of the cooling liquid is 300 L / h, and the flow rate parameter of the material pump is 0.5 t / h. After the treatment chamber and the refrigeration system are connected, the cooling device is first turned on, the pipeline of the refrigeration unit is circulated for about 5 min, the titanium alloy inner electrode surface of the treatment chamber has a small ice crystal layer or the cooling circulating system reaches the stable set temperature, the material pump is started, the grape pulp is conveyed, the grape pulp is treated in the treatment chamber, the high-voltage pulse power supply output is started, the feeding temperature of the grape pulp is measured by an optical fiber thermometer, the discharging temperature is 20°C, the overall stable state is met, the flow rate of the pump does not need to be adjusted, after the treatment is completed, the high-voltage pulse power supply output is turned off, the material conveying pump is stopped, and the cooling system is turned off. The polyphenol content of the obtained grape pulp after treatment is measured, and the polyphenol content is 1073 mg / L, and the polyphenol content of the grape pulp without high-voltage pulse electric field treatment is 1058 mg / L.

[0044] Comparative example 2 (the effect is not obvious when the frequency is lower than 200 Hz)

[0045] As shown in Figure 1 A continuous high-voltage pulse electric field treatment method for strengthening extraction of functional active ingredients of berry fruits, the implementation of the method needs the auxiliary matching of related devices such as a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3, and a cooling device 4. The various system devices are connected through pipelines, and the connection and fixation are carried out in the shown order. Figure 1The connection and fixation are carried out in the shown order, 200 kg of rose grape is purchased on the market, after the grape stems and leaves are removed, the grape is crushed by a screw press, the initial conductivity of the grape pulp is measured by a conductivity meter, the initial temperature of the grape pulp material is 20°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 0.6 kV / cm, the frequency is 190 Hz, the pulse width is 10 μs, the bipolar symmetric square wave pulse is set, and the parameters are started after being set. The grape pulp is conveyed to the treatment chamber device 3 through the pipeline by the material conveying pump 2, the feeding mode is feeding from the bottom and discharging from the top. The cooling device of the treatment chamber is connected to the refrigeration unit, the refrigeration unit uses 8% alcohol water solution as the cooling circulating liquid, the cooling liquid temperature is -5°C, the cooling liquid flow rate is 300 L / h, and the material pump flow parameter is 0.5 t / h. After the treatment chamber and the refrigeration system are connected, the cooling device is first turned on, the pipeline of the refrigeration unit is circulated for about 5 min, the titanium alloy inner electrode surface of the treatment chamber has a small ice crystal layer or the cooling circulating system cooling liquid temperature reaches the stable set temperature, the material pump is started, the grape pulp is conveyed, the grape pulp is started after the treatment chamber, the high-voltage pulse power supply output is started, the grape pulp feeding temperature is measured by an optical fiber thermometer, the discharging temperature is 20°C, the overall stable state is met, and the pump flow rate does not need to be adjusted, after the treatment is finished, the high-voltage pulse power supply output is turned off, the material conveying pump is stopped, and the cooling system is turned off. The obtained grape pulp polyphenol composition is measured after the treatment, the polyphenol content is 1086 mg / L, and the polyphenol content of the grape pulp without high-voltage pulse electric field treatment is 1049 mg / L.

[0046] Example 2

[0047] As Figure 1 shown, a continuous high-voltage pulse electric field treatment method for strengthening extraction of functional active ingredients of berry fruits is provided, and the implementation of the method needs the auxiliary matching of a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3, a cooling device 4 and other related devices. The various system devices are connected through pipelines, and the pipelines are connected to the high-voltage pulse power supply 1, the material conveying pump 2, the treatment chamber device 3 and the cooling device 4. Figure 1The connection and fixation are carried out in the shown order, 200 kg of rose grape is purchased on the market, after the grape stems and leaves are removed, the grape is crushed by a screw press, the initial conductivity of the grape pulp is measured by a conductivity meter, the initial temperature of the grape pulp material is 20°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 0.7 kV / cm, the frequency is 500 Hz, the pulse width is 8 μs, the bipolar symmetric square wave pulse is set, and the parameters are started after being set. The grape pulp is conveyed to the treatment chamber device 3 through the pipeline by the material conveying pump 2, the feeding mode is feeding from the bottom and discharging from the top. The cooling device port (No. 3) of the treatment chamber is connected to the refrigeration unit, the refrigeration unit uses 8% alcohol water solution as the cooling circulating liquid, the cooling liquid temperature is -8°C, the cooling liquid flow rate is 350 L / h, and the material pump flow parameter is 0.6 t / h. After the treatment chamber and the refrigeration system are connected, the cooling device is first turned on, the refrigeration unit pipeline circulates for about 5 min, the titanium alloy inner electrode surface of the treatment chamber has a small ice crystal layer or the cooling circulating system cooling liquid temperature reaches the stable set temperature, the material pump is started, the grape pulp is conveyed, the grape pulp is started after the treatment chamber, the high-voltage pulse power supply output is started, the grape pulp feeding temperature is measured by an optical fiber thermometer, the discharge temperature is 20°C, the overall stable state is met, and the pump flow rate does not need to be adjusted, after the treatment is finished, the high-voltage pulse power supply output is turned off, the material conveying pump is stopped, and the cooling system is turned off. The obtained grape pulp polyphenol composition is measured after the treatment, the polyphenol content is 1253 mg / L, and the polyphenol content without high-voltage pulse electric field treatment is 1056 mg / L.

[0048] Example 3

[0049] As Figure 1 shown, a continuous high-voltage pulse electric field treatment method for strengthening extraction of functional active ingredients of berry fruits is provided, and the implementation of the method needs the auxiliary matching of a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3, a cooling device 4 and other related devices. The various system devices are connected through pipelines and wires, and the system devices are connected to the high-voltage pulse power supply 1 through the pipeline and the wire. Figure 1The connections were made in the order shown. 200 kg of Muscat grapes were purchased from the market. After destemming and removing leaves, the grapes were crushed using a screw press. The initial conductivity of the grape pulp was measured to be 1812 μS / cm using a conductivity meter. The initial temperature of the grape pulp was 25℃. The high-voltage pulse power supply output parameters were set as follows: pulse electric field strength 0.8 kV / cm, frequency 500 Hz, pulse width 9 μs, bipolar symmetrical square wave pulse. After setting the parameters, the system was ready to start. The grape pulp was transported to the processing chamber device 3 via a pipeline by material conveying pump 2. The feeding method was bottom feeding and top discharging. The cooling device inlet of the processing chamber was connected to a refrigeration unit. The refrigeration unit used an 8% alcohol aqueous solution as the cooling circulating fluid. The cooling liquid temperature was -7℃, the cooling liquid flow rate was 410 L / h, and the material pump flow rate was 1.0 t / h. After connecting the processing chamber to the refrigeration system, first turn on the cooling device and run the refrigeration unit's piping for about 5 minutes. Once small ice crystals appear on the surface of the titanium alloy inner electrode in the processing chamber or the coolant temperature in the cooling circulation system reaches a stable set temperature, start the material pump to deliver the grape pulp. After the grape pulp passes through the processing chamber, start the high-voltage pulse power supply output. Use a fiber optic thermometer to measure the grape pulp inlet temperature; both the inlet and outlet temperatures are 25°C, meeting the overall stability requirements. No pump flow rate adjustment is needed. After processing, turn off the high-voltage pulse power supply output, stop the material delivery pump, and shut down the cooling system. The polyphenol content of the grape pulp obtained after processing is measured to be 1291 mg / L, while the polyphenol content without high-voltage pulse electric field treatment is 1087 mg / L.

[0050] Example 4

[0051] like Figure 1 As shown, a method for enhanced extraction of functional active ingredients from berries using a continuous high-voltage pulsed electric field is described. This method requires the auxiliary support of a high-voltage pulsed power supply 1, a material conveying pump 2, a processing chamber 3, and a cooling device 4. The various system devices are connected via... Figure 1The connection and fixation are carried out in the shown order, 200 kg of rose grape is purchased on the market, after the grape stems and leaves are removed, the grape is crushed by a screw press, the initial conductivity of the grape pulp is measured by a conductivity meter, the initial temperature of the grape pulp is 28°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 1.2 kV / cm, the frequency is 400 Hz, the pulse width is 8 μs, the bipolar symmetric square wave pulse is set, and the parameters are started after being set. The grape pulp is conveyed to the treatment chamber device 3 through the pipeline by the material conveying pump 2, the feeding mode is feeding from the bottom and discharging from the top. The cooling device of the treatment chamber is connected to the refrigeration unit, the refrigeration unit uses 8% alcohol water solution as the cooling circulating liquid, the cooling liquid temperature is -6°C, the cooling liquid flow rate is 385 L / h, and the material pump flow parameter is 1.2 t / h. After the treatment chamber and the refrigeration system are connected, the cooling device is first turned on, the refrigeration unit pipeline circulates for about 5 min, the titanium alloy inner electrode surface of the treatment chamber has a small ice crystal layer or the cooling circulating system cooling liquid temperature reaches the stable set temperature, the material pump is started, the grape pulp is conveyed, the grape pulp is treated in the treatment chamber, the high-voltage pulse power supply output is started, the grape pulp feeding temperature is measured by an optical fiber thermometer, the grape pulp feeding temperature is 28°C, the discharge temperature is 28°C, the overall stable state is met, and the pump flow rate does not need to be adjusted, after the treatment is completed, the high-voltage pulse power supply output is turned off, the material conveying pump is stopped, and the cooling system is turned off. The polyphenol content of the obtained grape pulp after treatment is measured, and the polyphenol content is 1274 mg / L, and the polyphenol content of the grape pulp without high-voltage pulse electric field treatment is 1030 mg / L.

[0052] Example 5

[0053] As Figure 1 shown, a continuous high-voltage pulse electric field treatment method for strengthening extraction of functional active ingredients of berry fruits is provided, and the implementation of the method requires the auxiliary matching of related devices such as a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3, and a cooling device 4. The various system devices are connected through pipelines, and the pipelines are connected to the treatment chamber device 3. Figure 1The connection and fixation are carried out in the shown order, 200 kg of rose grape is purchased on the market, after the grape stems and leaves are removed, the grape is crushed by a screw press, the initial conductivity of the grape pulp is measured by a conductivity meter, the initial temperature of the grape pulp material is 30°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 1.5 kV / cm, the frequency is 400 Hz, the pulse width is 7 μs, the bipolar symmetric square wave pulse is set, and then the high-voltage pulse power supply is started. The grape pulp is conveyed to the treatment chamber device 3 through the pipeline by the material conveying pump 2, the feeding mode is feeding from the bottom and discharging from the top. The cooling device of the treatment chamber is connected to the refrigeration unit, the refrigeration unit uses 8% alcohol water solution as the cooling circulating liquid, the temperature of the cooling liquid is -8°C, the flow rate of the cooling liquid is 500 L / h, and the flow rate parameter of the material pump is 1.5 t / h. After the treatment chamber and the refrigeration system are connected, the cooling device is first turned on, the pipeline of the refrigeration unit is circulated for about 5 min, the titanium alloy inner electrode surface of the treatment chamber has a small ice crystal layer or the temperature of the cooling circulating system reaches the stable set temperature, the material pump is started, the grape pulp is conveyed, the grape pulp is treated in the treatment chamber, the high-voltage pulse power supply output is started, the feeding temperature of the grape pulp is measured by an optical fiber thermometer, the feeding temperature of the grape pulp is 30°C, the discharging temperature of the grape pulp is 30°C, the overall stable state is met, the flow rate of the pump does not need to be adjusted, after the treatment is completed, the high-voltage pulse power supply output is turned off, the material conveying pump is stopped, and the cooling system is turned off. The polyphenol content of the obtained grape pulp after the treatment is measured, and the polyphenol content is 1482 mg / L, and the polyphenol content of the grape pulp without high-voltage pulse electric field treatment is 1074 mg / L.

[0054] Example 6

[0055] As Figure 1 shown, a method for continuously treating functional active ingredients of berry fruits by high-voltage pulse electric field to strengthen extraction, the implementation of the method needs the auxiliary matching of related devices such as a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3, and a cooling device 4. The various system devices are connected through pipelines, and the pipeline connection is shown in the figure. Figure 1The connection and fixation are carried out in the shown order, 200 kg of rose grape is purchased on the market, after the grape stems and leaves are removed, the grape is crushed by a screw press, the initial conductivity of the grape pulp is measured by a conductivity meter, the initial conductivity of the grape pulp is 1800 μS / cm, a small amount of brine is added to adjust the conductivity to 1957 μS / cm, the initial temperature of the grape pulp material is 15°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 2.0 kV / cm, the frequency is 300 Hz, the pulse width is 6 μs, the bipolar symmetric square wave pulse is set, and the parameters are started after being set. The grape pulp is conveyed to the treatment chamber device 3 through the pipeline by the material conveying pump 2, the feeding mode is feeding from the bottom and discharging from the top. The cooling device of the treatment chamber is connected to the refrigeration unit, the refrigeration unit uses 8% alcohol water solution as the cooling circulating liquid, the cooling liquid temperature is -8°C, the cooling liquid flow rate is 480 L / h, and the material pump flow parameter is 1.8 t / h. After connecting the treatment chamber and the refrigeration system, first, the cooling device is turned on, the refrigeration unit pipeline circulates for about 5 min, the titanium alloy inner electrode surface of the treatment chamber appears small ice crystal layer or the cooling circulating system cooling liquid temperature reaches the stable set temperature, the material pump is started, the grape pulp is conveyed, the grape pulp is treated in the treatment chamber, the high-voltage pulse power supply output is started, the grape pulp feeding temperature is measured by an optical fiber thermometer, the feeding temperature is 15°C, the discharging temperature is 15°C, the overall stable state is met, and the pump flow rate does not need to be adjusted, after the treatment is completed, the high-voltage pulse power supply output is turned off, the material conveying pump is stopped, and the cooling system is turned off. The obtained grape pulp polyphenol composition is measured after the treatment, the polyphenol content is 1376 mg / L, and the polyphenol content without high-voltage pulse electric field treatment is 1065 mg / L.

[0056] Example 7

[0057] As Figure 1 shown, a continuous high-voltage pulse electric field treatment method for strengthening extraction of functional active ingredients of berry fruits is provided, and the implementation of the method needs the auxiliary matching of related devices such as a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3, and a cooling device 4. The various system devices are connected through pipelines, and the pipelines are connected to the devices through the pipeline connection device. Figure 1The connection and fixation are carried out in the shown order, 200 kg of rose grape is purchased on the market, after the grape stems and leaves are removed, the grape is crushed by a screw press, the initial conductivity of the grape pulp is measured by a conductivity meter, the initial conductivity of the grape pulp is 1831 μS / cm, a small amount of sodium chloride is added to adjust the conductivity to 2230 μS / cm, the initial temperature of the grape pulp material is 15°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 2.2 kV / cm, the frequency is 200 Hz, the pulse width is 9 μs, the bipolar symmetric square wave pulse is set, and the parameters are started after being set. The grape pulp is conveyed to the treatment chamber device 3 through the pipeline by the material conveying pump 2, the feeding mode is feeding from the bottom and discharging from the top. The cooling device of the treatment chamber is connected to the refrigeration unit, the refrigeration unit uses 8% alcohol water solution as the cooling circulating liquid, the cooling liquid temperature is -8°C, the cooling liquid flow rate is 450 L / h, and the material pump flow parameter is 2.5 t / h. After the treatment chamber and the refrigeration system are connected, the cooling device is first turned on, the refrigeration unit pipeline circulates for about 5 min, the titanium alloy inner electrode surface of the treatment chamber appears small ice crystal layer or the cooling circulating system cooling liquid temperature reaches the stable set temperature, the material pump is started, the grape pulp is conveyed, the grape pulp is treated in the treatment chamber, the high-voltage pulse power supply output is started, the grape pulp feeding temperature is measured by an optical fiber thermometer, the feeding temperature is 15°C, the discharging temperature is 15°C, the overall stable state is met, and the pump flow rate does not need to be adjusted, after the treatment is completed, the high-voltage pulse power supply output is turned off, the material conveying pump is stopped, and the cooling system is turned off. The polyphenol content of the obtained grape pulp after treatment is measured, and the polyphenol content is 1345 mg / L, and the polyphenol content without high-voltage pulse electric field treatment is 1071 mg / L.

[0058] Example 8

[0059] As Figure 1 shown, a continuous high-voltage pulse electric field treatment method for strengthening extraction of functional active ingredients of berry fruits is provided, and the implementation of the method requires the auxiliary matching of related devices such as a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3, and a cooling device 4. The various system devices are connected through pipelines, and the pipelines are connected to the treatment chamber device 3. Figure 1The connection and fixation are carried out in the shown order, 200 kg of rose grape is purchased on the market, after the grape stems and leaves are removed, the grape is crushed by a screw press, the initial conductivity of the grape pulp is measured by a conductivity meter, the conductivity is adjusted to 3120 μS / cm by adding a small amount of sodium chloride, the initial temperature of the grape pulp material is 18°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 2.5 kV / cm, the frequency is 300 Hz, the pulse width is 6 μs, the bipolar symmetric square wave pulse is set, and the parameters are started after being set. The grape pulp is conveyed to the treatment chamber device 3 through the pipeline by the material conveying pump 2, the feeding mode is feeding from the bottom and discharging from the top. The cooling device of the treatment chamber is connected to the refrigeration unit, the refrigeration unit uses 8% alcohol water solution as the cooling circulating liquid, the cooling liquid temperature is -8°C, the cooling liquid flow rate is 435 L / h, and the material pump flow parameter is 3.0 t / h. After connecting the treatment chamber and the refrigeration system, first, the cooling device is turned on, the refrigeration unit pipeline circulates for about 5 min, the titanium alloy inner electrode surface of the treatment chamber appears small ice crystal layer or the cooling circulating system cooling liquid temperature reaches the stable set temperature, the material pump is started, the grape pulp is conveyed, the grape pulp is treated in the treatment chamber, the high-voltage pulse power supply output is started, the grape pulp feeding temperature is measured by an optical fiber thermometer, the feeding temperature is 18°C, the discharging temperature is 18°C, the overall stable state is met, and the pump flow rate does not need to be adjusted, after the treatment is completed, the high-voltage pulse power supply output is turned off, the material conveying pump is stopped, and the cooling system is turned off. After the treatment, the polyphenol content of the obtained grape pulp is measured, the polyphenol content is 1307 mg / L, and the polyphenol content of the grape pulp without high-voltage pulse electric field treatment is 1060 mg / L. The treatment intensity is greater than 2.5 kV / cm, and the polyphenol yield is lower than that of the treatment intensity of 2.2 kV / cm.

[0060] Example 9

[0061] As Figure 1 shown, a continuous high-voltage pulse electric field treatment method for strengthening the extraction of functional active ingredients of berry fruits is provided, which requires the auxiliary matching of related devices such as a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3, and a cooling device 4. The various system devices are connected through pipelines, and the connection and fixation are carried out in the shown order. Figure 1The connection and fixation are carried out in the order shown, 200 kg of mulberry fruits are crushed by a screw press, the initial conductivity of the mulberry pulp is measured to be 3670 μS / cm, the initial temperature of the material is 18°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 1.1 kV / cm, the frequency is 200 Hz, the pulse width is 8 μs, the bipolar symmetric square wave pulse is set, and the parameters are set. After starting, the mulberry pulp is transported to the treatment chamber device 3 through the pipeline by the material conveying pump 2, the feeding mode is from the bottom to the top, and the cooling device of the treatment chamber is connected to the refrigeration unit. The refrigeration unit uses 10% alcohol aqueous solution as the cooling circulating liquid, the temperature of the cooling liquid is -8°C, the flow rate of the cooling liquid is 450 L / h, and the flow rate parameter of the material pump is 1.0 t / h. After connecting the treatment chamber and the refrigeration system, the cooling device is first turned on, the pipeline of the refrigeration unit is circulated for about 5 min, the titanium alloy inner electrode surface of the treatment chamber appears small ice crystal layer or the cooling circulating system reaches the stable set temperature, the material pump is started, the mulberry pulp is transported, the mulberry pulp is treated in the treatment chamber, the high-voltage pulse power supply is started, the feeding temperature of the grape pulp is measured to be 18°C by the optical fiber thermometer, the discharging temperature is 18°C, the overall stable state is met, and the pump flow rate does not need to be adjusted. After the treatment is completed, the high-voltage pulse power supply is turned off, the material conveying pump is stopped, and the cooling system is turned off. After the treatment, the polyphenol content is measured to be 2363 mg / L, and the polyphenol content without pulse electric field treatment is 1857 mg / L.

[0062] Example 10

[0063] As Figure 1 shown, a continuous high-voltage pulse electric field treatment method for strengthening the extraction of functional active ingredients of berry fruits is provided. The implementation of the method requires the auxiliary matching of related devices such as a high-voltage pulse power supply 1, a material conveying pump 2, a treatment chamber device 3, and a cooling device 4. The various system devices are connected through pipelines, and the pipelines are connected to the cooling device 4. ​The connection and fixation are carried out in the shown order, 200 kg of mulberry fruits are crushed by a screw press, the initial conductivity of the mulberry pulp is measured as 3320 μS / cm, the initial temperature of the material is 25°C, the output parameters of the high-voltage pulse power supply are set, the pulse electric field intensity is 2.0 kV / cm, the frequency is 200 Hz, the pulse width is 6 μs, the bipolar symmetric square wave pulse is used, and the parameters are set and started. The mulberry pulp is transported to the treatment chamber device 3 through the pipeline by the material delivery pump 2, the feeding mode is feeding from the bottom and discharging from the top. The cooling device of the treatment chamber is connected to the refrigeration unit, the refrigeration unit uses 10% alcohol water solution as the cooling circulating liquid, the temperature of the cooling liquid is -5°C, the flow rate of the cooling liquid is 500 L / h, and the flow rate parameter of the material pump is 3.0 t / h. After connecting the treatment chamber and the refrigeration system, the cooling device is turned on first, the pipeline of the refrigeration unit is circulated for about 5 min, the surface of the titanium alloy inner electrode of the treatment chamber has a small ice crystal layer or the temperature of the cooling circulating system reaches the stable set temperature, the material pump is started, the mulberry pulp is transported, the feeding temperature of the grape pulp is measured as 25°C by the optical fiber thermometer, the discharging temperature is 25°C, the overall stable state is met, the flow rate of the pump does not need to be adjusted, after the treatment is completed, the output of the high-voltage pulse power supply is turned off, the material delivery pump is stopped, and the cooling system is turned off. After the treatment, the polyphenol content is measured, the polyphenol content is 2250 mg / L, and the polyphenol content without pulse electric field treatment is 1894 mg / L.

[0064] In the aspect of electrode synchronous low-temperature treatment, the experiment compares the titanium ion concentration (determined by inductively coupled plasma mass spectrometry) in a 50 L sodium chloride solution with an electric conductivity of 4000 μS / cm under the conditions of no electrode refrigeration and with electrode refrigeration after continuous operation for 4 hours. The experimental results are shown in Table 1.

[0065] Table 1

[0066]

[0067] Under the conditions of an electric field intensity of 2.2 kV / cm, a bipolar pulse width of 10 μs, and a frequency of ≤500 Hz, the corrosion resistance of the electrode can be greatly improved by refrigerating the electrode. When the electric field intensity is greater than 2.2 kV / cm or the pulse width is ≥12 μs, the electrode will be corroded.

[0068] The above examples are only used to help understand the technical solutions of the present application and its core idea. It should be pointed out that for those skilled in the art, without departing from the principles of the present application, the present application can be improved and modified in many ways, and these improvements and modifications also fall within the protection scope of the claims of the present application.

Claims

1. A method for the enhanced extraction of functional active ingredients from berries by continuous high-voltage pulsed electric field treatment, characterized in that, The high-voltage pulse electric field connection extraction device and material control system is realized by a processing chamber device and a cooling device, the processing chamber device includes titanium alloy electrodes, stainless steel sealing plates arranged on both sides and an insulating support arranged in the middle, the titanium alloy electrodes are symmetrically embedded on the insulating support, the stainless steel sealing plates are fixed on the outer edge of the insulating support, the stainless steel sealing plates, the titanium alloy electrodes and the insulating support form a sandwich cavity as a whole, the bottom of the sandwich cavity is connected with the coolant inlet and the coolant outlet of the cooling device, the coolant enters the sandwich cavity through the coolant inlet to cool the titanium alloy electrodes, and then the coolant is discharged through the coolant outlet, and the high-voltage pulse power output end is connected with the titanium alloy electrodes to generate a high-voltage pulse electric field; the method comprises the following steps: after the processing chamber device is cooled to a set temperature by the cooling device, the output parameters of the high-voltage pulse power are set, the high-voltage pulse power generates a bipolar symmetric square wave, the pulse electric field parameters are set, the distance between the two titanium alloy electrodes is 100 mm, the pretreated berry material is transported to the processing chamber device, the berry material is subjected to high-voltage pulse electric field treatment, the feeding temperature and the discharging temperature of the berry material are the same, and the berry extraction liquid containing active ingredients is obtained; the coolant temperature in the cooling device is set to-5-8 DEG C, the coolant liquid flow rate is 300-500 L / h, the coolant is circulated for 5-10 min to reach a set temperature below 0 DEG C, and the electrode surface is covered with a thin layer of ice crystal; the pulse electric field parameters are as follows: the pulse electric field strength is 0.6-2.2 kV / cm, the frequency is 200-500 Hz, and the pulse width is 6-10 mu s; the titanium alloy electrode comprises a first titanium alloy electrode and a second titanium alloy electrode which are symmetrically embedded on the insulating support, the titanium alloy electrode material adopts Ti-0.2Pd corrosion-resistant high-hardness titanium alloy, the stainless steel sealing plate comprises a first stainless steel sealing plate and a second stainless steel sealing plate which are symmetrically arranged on both sides of the processing chamber device, the first stainless steel sealing plate, the first titanium alloy electrode and the insulating support form a first sandwich cavity as a whole, the second stainless steel sealing plate, the second titanium alloy electrode and the insulating support form a second sandwich cavity as a whole, the first sandwich cavity and the second sandwich cavity are connected through a communication hole, the communication hole is on the insulating support and connects the first sandwich cavity and the second sandwich cavity, the bottom of the first sandwich cavity is connected with the coolant inlet, and the bottom of the second sandwich cavity is connected with the coolant outlet; the processing chamber device has a round-to-square structure, and a groove for installing a sealing ring is arranged between the titanium alloy electrode and the insulating support.

2. The method of claim 1, wherein, A first threaded screw is welded in the middle of the first titanium alloy electrode, the first threaded screw is connected with the first stainless steel sealing plate through threads, and is fixed through a first inner locking nut; a second threaded screw is welded in the middle of the second titanium alloy electrode, the second threaded screw is connected with the second stainless steel sealing plate through threads, and is fixed through a second inner locking nut.

3. The method of claim 1, wherein, The first stainless steel sealing plate is provided with a first stainless steel sealing plate screw hole, and the first stainless steel sealing plate is fixed on the outer edge of the insulating support through a first sealing insulating screw.

4. The method of claim 1, wherein, The berry material has an electric conductivity of 1800-4000 µS / cm.

5. The method of claim 4, wherein, The berry material is grape or mulberry.

6. The method of claim 1, wherein, The berry material feeding temperature is 15-30℃, and the berry material discharging temperature is consistent with the feeding temperature by adjusting the electrode refrigerant liquid flow rate and temperature and the material feeding speed, that is, the material is not heated during the processing. The berry material has an electric conductivity of 1800-4000 µS / cm. The berry material is grape or mulberry. The berry material feeding temperature is 15-30℃, and the berry material discharging temperature is consistent with the feeding temperature by adjusting the electrode refrigerant liquid flow rate and temperature and the material feeding speed, that is, the material is not heated during the processing.

Images