An electronic e-liquid with a refreshing snail flavor and its preparation method

Abstract

This invention relates to an e-liquid with a refreshing snail flavor and its preparation method, belonging to the field of e-liquid technology. The e-liquid prepared by this invention has a refreshing and long-lasting flavor, a delicate and ethereal aroma, is mild and non-irritating, and has a rich taste that leaves a lasting impression. The e-liquid of this invention comprises a refreshing extract, tobacco flavoring, tobacco leaf extract, snail flavoring, medicinal flavoring agent, agar, glycerin, and olive oil. This e-liquid uses agar and olive oil as flavor-locking agents, which not only preserves the snail flavor for a long time but also provides a subtle, refreshing plant sensation upon closer tasting, giving a sense of tranquility and relaxation, like being in nature. The addition of the medicinal flavoring agent, when mixed with the e-liquid, can relieve dizziness, headaches, and reduce swelling caused by prolonged sitting or colds. This not only preserves the flavor of e-cigarettes but also enriches the flavor options currently available on the market and reduces the health risks associated with e-cigarettes.

Description

Technical Field

[0001] This invention belongs to the field of electronic e-liquid technology, and relates to an electronic e-liquid with a refreshing snail flavor and its preparation method. Background Technology

[0002] Currently, there are relatively few flavors and varieties of e-liquids, and the aroma components of e-liquids are difficult to preserve for a long time. The best ones can only be stored for about six months, and the "smoke flavor" is very weak or almost non-existent, which greatly affects the quality of e-cigarettes. This is a common problem reported by users.

[0003] To address the current technical challenges, there is a need to develop an e-liquid that can retain the "smoke flavor" for an extended period without causing harm to the human body. Such an e-liquid should also have the effect of relieving the user's mood. Summary of the Invention

[0004] In response to the existing technical problems, this invention provides an electronic e-liquid with a refreshing snail flavor. This electronic e-liquid can not only retain the "smoke flavor" for a long time without affecting the user's experience, but also has the characteristics of a fresh plant flavor and medicinal effects.

[0005] The objective of this invention can be achieved through the following technical solutions:

[0006] An e-cigarette liquid with a refreshing snail flavor, the e-cigarette liquid comprising the following ingredients in parts by weight:

[0007] 20-30 parts by weight of fresh extract, 1-5 parts by weight of tobacco flavoring, 10-15 parts by weight of tobacco leaf extract, 5-10 parts by weight of snail flavoring, 10-15 parts by weight of medicinal flavoring agent, 5-10 parts by weight of agar, 30-50 parts by weight of organic solvent and 5-15 parts by weight of olive oil;

[0008] The refreshing extracts include five-finger peach extract, lemon extract, and aloe vera extract; the snail flavoring includes snail extract, ginger extract, perilla extract, sugarcane juice, red chili extract, mushroom powder, and cardamom extract; the medicinal flavorings include medicinal plant extracts, tangerine peel extract, and honeysuckle granules.

[0009] The preparation method of the Five-Finger Peach Extract includes the following steps:

[0010] The roots of *Ficus hirta* were cut, cleaned, dried, and crushed. Then, a 50%-90% ethanol solution was added for extraction for 0.5-2 hours. After extraction, the solvent was removed to obtain a crude extract of *Ficus hirta*. The crude extract was then subjected to Soxhlet extraction with a 20%-90% ethanol solution. The extract was collected and concentrated under reduced pressure to obtain the *Ficus hirta* extract.

[0011] In particular, extracting *Ficus hirta* using an ethanol solution allows for the efficient extraction of psoralen, bergamot lactone, flavonoids, and coumarins from the ethanol solution. Furthermore, Soxhlet extraction ensures a purer extract and is a simple and safe procedure. *Ficus hirta* extract possesses antibacterial and anti-inflammatory properties, can improve respiratory function, relieve coughs and asthma, enhance immune function, protect the gastrointestinal tract and liver, and also has antioxidant, anti-aging, nerve-soothing, spleen-strengthening, and lung-tonifying effects.

[0012] The refreshing extract in the e-liquid of this invention is prepared by mixing five-finger peach extract, lemon extract, and aloe vera extract. Among them, five-finger peach extract not only has medicinal effects such as strengthening the spleen and lungs, reducing swelling and antibacterial properties, but also combines with the active substances in lemon extract and aloe vera extract, so that the prepared e-liquid has both medicinal properties and the effects of freshening breath and relaxing nerves. It makes users feel as if they are surrounded by the fragrance of flowers and fruits in nature, which can better refresh and relieve mental stress, and the "smoke smell" is fresh.

[0013] The preparation method of the snail extract includes the following steps:

[0014] Step 1: Add the dried snail meat, chili powder, and Sichuan peppercorn powder to boiling water and simmer, stirring constantly, for 30-40 minutes. Then filter to obtain snail liquid and set aside.

[0015] Step 2: Add the obtained snail liquid to water and continue to boil at a high temperature of 100-150℃. Add a thickener and boil until it becomes viscous. Add olive oil and concentrate at a low temperature of 50-60℃ for 2-3 hours. Dry to obtain snail extract.

[0016] Specifically, the thickener includes at least one selected from guar gum, carrageenan, sodium alginate, agar, and sodium carboxymethyl cellulose, preferably sodium alginate.

[0017] Furthermore, snail extract primarily consists of nutrients such as protein and vitamin A. As an ingredient in e-liquid, its inhalation can enhance the body's immunity. Chili powder and Sichuan peppercorn powder are added during the snail extraction process. These ingredients not only remove the fishy smell of the snails but also preserve the pure flavor of the e-liquid. The addition of thickeners and olive oil during the extraction process further concentrates the snail flavor. The olive oil reacts with the components in the snails to form an oil film, which better locks in the snail flavor, resulting in a richer taste for the e-liquid. As an e-liquid ingredient, snail extract not only preserves its flavor for a long time, but snails are also considered a cold-natured substance. Their active substances can help alleviate internal heat and detoxification, relieve pain and swelling, and may further prevent the occurrence of diseases.

[0018] The snail-flavored essence in the e-cigarette liquid of this invention includes snail extract, ginger extract, perilla extract, sugarcane juice, red chili extract, mushroom powder, and cardamom extract. The snails are cleaned snails caught in farmland or ditches. The protein and cellulose A extracted from them can improve human immunity. In addition, ginger extract, perilla extract, sugarcane juice, and red chili extract are added as flavoring agents, which not only remove the fishy smell of snails, but also have a light fragrance. Combined with mushroom powder and cardamom extract, the flavor of the e-cigarette is further enhanced, which can lock in the fragrance of snails and plants, so that the aroma of the e-cigarette can be retained for a longer time.

[0019] The preparation method of the medicinal plant extract includes the following steps:

[0020] (1) The picked medicinal plants are washed clean, dried and chopped, then soaked in water, refluxed and boiled for 2-4 hours, filtered, cooled and centrifuged, filtered, and the supernatant is freeze-dried to obtain the medicinal plant powder for later use.

[0021] (2) Weigh the obtained medicinal plant powder and add it to 10mM pH4.0 ammonium acetate buffer solution. Adjust the pH to 4.0-7.0, stir to dissolve, and then load it onto a cellulose chromatography column. Use different concentrations and different pH ammonium acetate buffer solutions to perform gradient elution in sequence to separate crude active substances. Continue to elute with phosphate buffer solution to collect the active substances of medicinal plants.

[0022] (3) Soak the active substances of the medicinal plant in a hot ethanol solution and stir to extract for 1-3 hours. After extraction, concentrate under reduced pressure to obtain the medicinal plant extract.

[0023] Specifically, the concentration gradient of the ammonium acetate buffer solution is 10 mM pH 4.0, 100 mM pH 5.0, and 200 mM pH 7.0. Using different concentration gradients of ammonium acetate buffer solution for elution is to better elute the active ingredients from the medicinal plants.

[0024] Specifically, the medicinal plants include at least one of dandelion, *Clematis chinensis*, mugwort, and *Gastrodia elata*, preferably dandelion and *Clematis chinensis*. Both of these medicinal plants have the effects of clearing heat and detoxifying, and promoting diuresis and reducing swelling. Extracting these medicinal components for use in e-cigarette liquid helps alleviate heat toxicity, clear internal heat evil, and reduce the harm caused by inhaling e-cigarettes. Furthermore, it has the effect of eliminating edema and promoting urination, and has a certain improvement effect on problems such as difficulty urinating and edema. The inhaled components can be quickly excreted through urine, so that e-cigarettes not only bring pleasure to the user but also have medicinal effects, achieving a double effect.

[0025] The medicinal flavoring agent in the e-cigarette liquid of this invention uses dandelion and purslane as medicinal plant extracts, combined with tangerine peel extract and honeysuckle granules. All of these are plant-based medicinal materials with a fresh aroma. When mixed and added to the e-cigarette liquid as a medicinal flavoring agent, it not only retains its own fragrance, but also does not affect the tobacco flavor of the e-cigarette liquid, and also has certain medicinal effects.

[0026] The tobacco extract is a Zimbabwean tobacco extract; the tobacco flavoring includes at least one of ethyl acetate, isoamyl acetate, benzoic acid, phenylacetic acid, citric acid, limonene, and myrcene. Limonene is preferably used as the tobacco flavoring, as it provides a fresh, sweet, and pleasant taste, giving tobacco products different taste and aroma effects. When used in combination with the refreshing extract, it gives users a refreshing feeling of being in nature amidst a busy life, better pleasing the mind and relieving mental stress.

[0027] The organic solvent includes at least one of glycerol, propylene glycol and ethanol, preferably glycerol. Glycerol has excellent solubility and is non-toxic. As a solvent for e-cigarette liquid, glycerol can make the components of e-cigarette mix more evenly and does not cause harm to the user's health.

[0028] A method for preparing an electronic e-liquid with a refreshing snail flavor, the method comprising the following steps:

[0029] By weight, the refreshing extract and flavoring agent are dissolved in water and heated at 80-100℃ for 10-15 minutes. The tobacco flavoring and tobacco leaf extract are added and stirred evenly. The mixture is then heated to 120-150℃ and refluxed for 10-20 minutes. The snail flavoring is then dissolved in an organic solvent and added to the reflux reaction while stirring. After the addition is complete, the mixture is heated to 180-200℃ and refluxed for 10-20 minutes. The refluxed product is dissolved in 10-50 mL of organic solvent and filtered to obtain the electronic cigarette liquid.

[0030] The beneficial effects of this invention are:

[0031] (1) Compared with the prior art, the present invention uses tobacco flavoring and tobacco leaf extract as the base material of e-liquid, and adds snail flavoring and plant-based medicinal flavoring as "smoke flavor" preservatives and medicinal additives for e-liquid. This not only preserves the flavor of e-cigarettes for a long time, but also has the effects of strengthening the spleen and lungs, antibacterial and anti-inflammatory, and relieving fatigue. Fresh extracts are used as the overall brightener of e-liquid. E-cigarettes prepared with various floral and fruity plant extracts not only bring fresh breath to users to a certain extent, but also effectively prevent and reduce the irritation of e-cigarette smoke to users' throats and lungs, improve users' comfort, and better relax users' mood.

[0032] (2) Compared with the prior art, this invention uses olive oil and agar as flavor-locking agents for e-liquid. Agar is a polysaccharide with good water solubility and gelling properties, which can form a stable colloidal system in e-liquid, making the texture of e-liquid more delicate and uniform, while enhancing the viscosity and stability of e-liquid and preventing separation and sedimentation. Agar can also interact with other components in e-liquid to improve overall stability, prevent oxidation and volatilization of e-liquid, thereby extending the service life of e-liquid and maintaining the quality of aroma. Olive oil contains a large amount of unsaturated fatty acids, which have good antioxidant properties and can help slow down the oxidation process of e-liquid, thereby maintaining freshness and taste. Olive oil also has good viscosity and stability, and can form a thin oil film on the surface of e-liquid. This oil film can prevent oxygen and moisture in the air from contacting food, thereby preventing food spoilage and maintaining food flavor. Olive oil not only blends snail flavoring with fresh extracts and medicinal agents evenly, but also has a long-lasting flavor-locking function. When used in combination with agar, the resulting e-liquid not only has a unique flavor, but can also retain this unique flavor for a long time, solving the problem of single flavor and short shelf life of e-liquids on the market. Detailed Implementation

[0033] To further illustrate the technical means and effects adopted by the present invention to achieve its intended purpose, the specific implementation methods, features, and effects of the present invention are described in detail below with reference to embodiments. The reagents and medicines used in the present invention are all commercially available or can be prepared in-house. Example 1

[0034] An e-cigarette liquid with a refreshing snail flavor, the e-cigarette liquid comprising the following ingredients in parts by weight:

[0035] 20 parts by weight of fresh extract, 5 parts by weight of limonene, 15 parts by weight of Zimbabwe tobacco extract, 5 parts by weight of snail flavoring, 10 parts by weight of medicinal flavoring agent, 7 parts by weight of agar, 30 parts by weight of glycerin and 10 parts by weight of olive oil;

[0036] The refreshing extracts include five-finger peach extract, lemon extract, and aloe vera extract; the snail flavoring includes snail extract, ginger extract, perilla extract, sugarcane juice, red chili extract, mushroom powder, and cardamom extract; the medicinal flavorings include medicinal plant extracts, tangerine peel extract, and honeysuckle granules.

[0037] The preparation method of the Five-Finger Peach Extract includes the following steps:

[0038] The roots of *Ficus hirta* were cut, cleaned, dried, and crushed. A 60% ethanol solution was added for extraction for 1.5 hours. After extraction, the solvent was removed by rotary evaporation to obtain a crude extract of *Ficus hirta*. The crude extract was then subjected to Soxhlet extraction with a 50% ethanol solution at 80°C. The extract was collected and concentrated under reduced pressure to obtain the *Ficus hirta* extract.

[0039] The preparation method of the snail extract includes the following steps:

[0040] Step 1: Add the dried snail meat, chili powder, and Sichuan peppercorn powder to boiling water and simmer while stirring. After simmering for 30 minutes, filter to obtain snail liquid and set aside.

[0041] Step 2: Add the obtained snail liquid to water and continue to boil at a high temperature of 150°C. Add the thickener sodium alginate and boil until it becomes viscous. Add olive oil and concentrate at a low temperature of 60°C for 3 hours. Dry to obtain snail extract.

[0042] The preparation method of the medicinal plant extract includes the following steps:

[0043] (1) Rinse the dandelion and Qingming grass thoroughly, dry them and chop them, then soak them in water, boil them under reflux for 3 hours, filter them, cool them and centrifuge them, filter them again, take the supernatant and freeze dry them to obtain the medicinal plant powder for later use.

[0044] (2) Weigh the obtained medicinal plant powder and add it to 10 mM pH 4.0 ammonium acetate buffer solution. Adjust the pH to 5.0, stir to dissolve, and then load it onto a cellulose chromatography column. Use ammonium acetate buffer solutions with concentration gradients of 10 mM pH 4.0, 100 mM pH 5.0 and 200 mM pH 7.0 to perform gradient elution in sequence to separate crude active substances. Continue to elute with phosphate buffer solution to collect the active substances of medicinal plants.

[0045] (3) Soak the active substances of the medicinal plant in a hot ethanol solution and stir for 3 hours. After the extraction is completed, concentrate under reduced pressure to obtain the medicinal plant extract.

[0046] A method for preparing an electronic e-liquid with a refreshing snail flavor, the method comprising the following steps:

[0047] By weight, the refreshing extract and the medicinal flavoring agent were dissolved in water and heated at 100°C for 15 minutes. The tobacco flavoring and tobacco leaf extract were added and stirred evenly. The mixture was then heated to 130°C and refluxed for 20 minutes. The snail flavoring was then dissolved in glycerin and added to the reflux reaction while stirring. After the addition was complete, the mixture was heated to 180°C and refluxed for 20 minutes. The refluxed product was dissolved in 30 mL of glycerin and filtered to obtain the electronic cigarette liquid. Example 2

[0048] An e-cigarette liquid with a refreshing snail flavor, the e-cigarette liquid comprising the following ingredients in parts by weight:

[0049] 25 parts by weight of fresh extract, 5 parts by weight of limonene, 15 parts by weight of Zimbabwe tobacco extract, 5 parts by weight of snail flavoring, 12 parts by weight of medicinal flavoring, 7 parts by weight of agar, 40 parts by weight of glycerin and 10 parts by weight of olive oil;

[0050] The refreshing extracts include five-finger peach extract, lemon extract, and aloe vera extract; the snail flavoring includes snail extract, ginger extract, perilla extract, sugarcane juice, red chili extract, mushroom powder, and cardamom extract; the medicinal flavorings include medicinal plant extracts, tangerine peel extract, and honeysuckle granules.

[0051] The preparation method of the Five-Finger Peach Extract includes the following steps:

[0052] The roots of *Ficus hirta* were cut, cleaned, dried, and crushed. A 60% ethanol solution was added for extraction for 1.5 hours. After extraction, the solvent was removed by rotary evaporation to obtain a crude extract of *Ficus hirta*. The crude extract was then subjected to Soxhlet extraction with a 50% ethanol solution at 80°C. The extract was collected and concentrated under reduced pressure to obtain the *Ficus hirta* extract.

[0053] The preparation method of the snail extract includes the following steps:

[0054] Step 1: Add the dried snail meat, chili powder, and Sichuan peppercorn powder to boiling water and simmer while stirring. After simmering for 30 minutes, filter to obtain snail liquid and set aside.

[0055] Step 2: Add the obtained snail liquid to water and continue to boil at a high temperature of 150°C. Add the thickener sodium alginate and boil until it becomes viscous. Add olive oil and concentrate at a low temperature of 60°C for 3 hours. Dry to obtain snail extract.

[0056] The preparation method of the medicinal plant extract includes the following steps:

[0057] (1) Rinse the dandelion and Qingming grass thoroughly, dry them and chop them, then soak them in water, boil them under reflux for 3 hours, filter them, cool them and centrifuge them, filter them again, take the supernatant and freeze dry them to obtain the medicinal plant powder for later use.

[0058] (2) Weigh the obtained medicinal plant powder and add it to 10 mM pH 4.0 ammonium acetate buffer solution. Adjust the pH to 5.0, stir to dissolve, and then load it onto a cellulose chromatography column. Use ammonium acetate buffer solutions with concentration gradients of 10 mM pH 4.0, 100 mM pH 5.0 and 200 mM pH 7.0 to perform gradient elution in sequence to separate crude active substances. Continue to elute with phosphate buffer solution to collect the active substances of medicinal plants.

[0059] (3) Soak the active substances of the medicinal plant in a hot ethanol solution and stir for 3 hours. After the extraction is completed, concentrate under reduced pressure to obtain the medicinal plant extract.

[0060] A method for preparing an electronic e-liquid with a refreshing snail flavor, the method comprising the following steps:

[0061] By weight, the refreshing extract and the medicinal flavoring agent were dissolved in water and heated at 100°C for 15 minutes. The tobacco flavoring and tobacco leaf extract were added and stirred evenly. The mixture was then heated to 130°C and refluxed for 20 minutes. The snail flavoring was then dissolved in glycerin and added to the reflux reaction while stirring. After the addition was complete, the mixture was heated to 180°C and refluxed for 20 minutes. The refluxed product was dissolved in 30 mL of glycerin and filtered to obtain the electronic cigarette liquid. Example 3

[0062] An e-cigarette liquid with a refreshing snail flavor, the e-cigarette liquid comprising the following ingredients in parts by weight:

[0063] 30 parts by weight of fresh extract, 5 parts by weight of limonene, 15 parts by weight of Zimbabwe tobacco extract, 5 parts by weight of snail flavoring, 15 parts by weight of medicinal flavoring agent, 7 parts by weight of agar, 50 parts by weight of glycerin and 10 parts by weight of olive oil;

[0064] The refreshing extracts include five-finger peach extract, lemon extract, and aloe vera extract; the snail flavoring includes snail extract, ginger extract, perilla extract, sugarcane juice, red chili extract, mushroom powder, and cardamom extract; the medicinal flavorings include medicinal plant extracts, tangerine peel extract, and honeysuckle granules.

[0065] The preparation method of the Five-Finger Peach Extract includes the following steps:

[0066] The roots of *Ficus hirta* were cut, cleaned, dried, and crushed. A 60% ethanol solution was added for extraction for 1.5 hours. After extraction, the solvent was removed by rotary evaporation to obtain a crude extract of *Ficus hirta*. The crude extract was then subjected to Soxhlet extraction with a 50% ethanol solution at 80°C. The extract was collected and concentrated under reduced pressure to obtain the *Ficus hirta* extract.

[0067] The preparation method of the snail extract includes the following steps:

[0068] Step 1: Add the dried snail meat, chili powder, and Sichuan peppercorn powder to boiling water and simmer while stirring. After simmering for 30 minutes, filter to obtain snail liquid and set aside.

[0069] Step 2: Add the obtained snail liquid to water and continue to boil at a high temperature of 150°C. Add the thickener sodium alginate and boil until it becomes viscous. Add olive oil and concentrate at a low temperature of 60°C for 3 hours. Dry to obtain snail extract.

[0070] The preparation method of the medicinal plant extract includes the following steps:

[0071] (1) Rinse the dandelion and Qingming grass thoroughly, dry them and chop them, then soak them in water, boil them under reflux for 3 hours, filter them, cool them and centrifuge them, filter them again, take the supernatant and freeze dry them to obtain the medicinal plant powder for later use.

[0072] (2) Weigh the obtained medicinal plant powder and add it to 10 mM pH 4.0 ammonium acetate buffer solution. Adjust the pH to 5.0, stir to dissolve, and then load it onto a cellulose chromatography column. Use ammonium acetate buffer solutions with concentration gradients of 10 mM pH 4.0, 100 mM pH 5.0 and 200 mM pH 7.0 to perform gradient elution in sequence to separate crude active substances. Continue to elute with phosphate buffer solution to collect the active substances of medicinal plants.

[0073] (3) Soak the active substances of the medicinal plant in a hot ethanol solution and stir for 3 hours. After the extraction is completed, concentrate under reduced pressure to obtain the medicinal plant extract.

[0074] A method for preparing an electronic e-liquid with a refreshing snail flavor, the method comprising the following steps:

[0075] By weight, the refreshing extract and the medicinal flavoring agent were dissolved in water and heated at 100°C for 15 minutes. The tobacco flavoring and tobacco leaf extract were added and stirred evenly. The mixture was then heated to 130°C and refluxed for 20 minutes. The snail flavoring was then dissolved in glycerin and added to the reflux reaction while stirring. After the addition was complete, the mixture was heated to 180°C and refluxed for 20 minutes. The refluxed product was dissolved in 30 mL of glycerin and filtered to obtain the electronic cigarette liquid. Example 4

[0076] An e-cigarette liquid with a refreshing snail flavor, the e-cigarette liquid comprising the following ingredients in parts by weight:

[0077] 30 parts by weight of fresh extract, 5 parts by weight of limonene, 15 parts by weight of Zimbabwe tobacco extract, 10 parts by weight of snail flavoring, 10 parts by weight of medicinal flavoring agent, 7 parts by weight of agar, 50 parts by weight of glycerin and 10 parts by weight of olive oil;

[0078] The refreshing extracts include five-finger peach extract, lemon extract, and aloe vera extract; the snail flavoring includes snail extract, ginger extract, perilla extract, sugarcane juice, red chili extract, mushroom powder, and cardamom extract; the medicinal flavorings include medicinal plant extracts, tangerine peel extract, and honeysuckle granules.

[0079] The preparation method of the Five-Finger Peach Extract includes the following steps:

[0080] The roots of *Ficus hirta* were cut, cleaned, dried, and crushed. A 60% ethanol solution was added for extraction for 1.5 hours. After extraction, the solvent was removed by rotary evaporation to obtain a crude extract of *Ficus hirta*. The crude extract was then subjected to Soxhlet extraction with a 50% ethanol solution at 80°C. The extract was collected and concentrated under reduced pressure to obtain the *Ficus hirta* extract.

[0081] The preparation method of the snail extract includes the following steps:

[0082] Step 1: Add the dried snail meat, chili powder, and Sichuan peppercorn powder to boiling water and simmer while stirring. After simmering for 30 minutes, filter to obtain snail liquid and set aside.

[0083] Step 2: Add the obtained snail liquid to water and continue to boil at a high temperature of 150°C. Add the thickener sodium alginate and boil until it becomes viscous. Add olive oil and concentrate at a low temperature of 60°C for 3 hours. Dry to obtain snail extract.

[0084] The preparation method of the medicinal plant extract includes the following steps:

[0085] (1) Rinse the dandelion and Qingming grass thoroughly, dry them and chop them, then soak them in water, boil them under reflux for 3 hours, filter them, cool them and centrifuge them, filter them again, take the supernatant and freeze dry them to obtain the medicinal plant powder for later use.

[0086] (2) Weigh the obtained medicinal plant powder and add it to 10 mM pH 4.0 ammonium acetate buffer solution. Adjust the pH to 5.0, stir to dissolve, and then load it onto a cellulose chromatography column. Use ammonium acetate buffer solutions with concentration gradients of 10 mM pH 4.0, 100 mM pH 5.0 and 200 mM pH 7.0 to perform gradient elution in sequence to separate crude active substances. Continue to elute with phosphate buffer solution to collect the active substances of medicinal plants.

[0087] (3) Soak the active substances of the medicinal plant in a hot ethanol solution and stir for 3 hours. After the extraction is completed, concentrate under reduced pressure to obtain the medicinal plant extract.

[0088] A method for preparing an electronic e-liquid with a refreshing snail flavor, the method comprising the following steps:

[0089] By weight, the refreshing extract and the medicinal flavoring agent were dissolved in water and heated at 100°C for 15 minutes. The tobacco flavoring and tobacco leaf extract were added and stirred evenly. The mixture was then heated to 130°C and refluxed for 20 minutes. The snail flavoring was then dissolved in glycerin and added to the reflux reaction while stirring. After the addition was complete, the mixture was heated to 180°C and refluxed for 20 minutes. The refluxed product was dissolved in 30 mL of glycerin, filtered, and the electronic cigarette liquid was obtained.

[0090] Comparative Example 1

[0091] Compared with Example 1, the difference of Comparative Example 1 is that snail extract was not added to the snail flavoring in Comparative Example 1.

[0092] Comparative Example 2

[0093] Compared with Example 1, the difference of Comparative Example 2 is that olive oil was not added in the preparation of the snail extract in Comparative Example 2.

[0094] Comparative Example 3

[0095] Compared with Example 1, the difference of Comparative Example 3 is that Comparative Example 3 does not add the refreshing extract.

[0096] Comparative Example 4

[0097] Compared with Example 1, the difference of Comparative Example 4 is that no medicinal flavoring agent was added in Comparative Example 4.

[0098] Comparative Example 5

[0099] Compared with Example 1, the difference in Comparative Example 5 is that no agarose gel was added in Comparative Example 5.

[0100] Comparative Example 6

[0101] Compared with Example 1, Comparative Example 6 differs in that it does not involve the addition of olive oil.

[0102] Comparative Example 7

[0103] Compared with Example 1, the difference of Comparative Example 7 is that olive oil was replaced with water in Comparative Example 7.

[0104] Take 2 mL of each of the e-liquids prepared in Examples 1-4 and Comparative Examples 1-7 and add them to the atomizer. Ask a professional e-cigarette user to evaluate the e-liquid.

[0105] The e-liquids from Examples 1-4 and Comparative Examples 1-7 were added to e-cigarette devices for sensory evaluation. Different scores (out of 15) were assigned to the varying degrees of impact on the sensory quality of the e-liquids based on different evaluation indicators, including irritation, satisfaction, characteristic style, tobacco aroma, fragrance, and vapor production. Irritation refers to the unpleasant sensation experienced by the sensory organs, including the mouth, throat, and nasal cavity, when inhaling the vapor produced by the e-liquid. Satisfaction refers to the overall sensory experience experienced by the sensory organs, including the strength, concentration, and aroma of the vapor produced by the e-liquid. Characteristic style refers to the main sensory differences in pleasure between one e-liquid and other similar e-liquids. Tobacco aroma refers to the aroma of tobacco perceived during the evaluation process. Fragrance refers to the degree of pleasantness perceived through smell and taste during the evaluation process. Vapor production refers to the amount of vapor produced by the e-liquid after atomization. Durability refers to the ability of the e-liquid to maintain its sensory quality over extended periods of use. The sensory quality evaluation indicators and scores for e-liquids are shown in Table 1.

[0106] Table 1 Sensory Quality Evaluation Indicators and Scoring Values ​​for E-liquids

[0107] Irritating satisfaction Characteristic Style Tobacco Scent aroma Smoke volume Persistence Example 1 5 14 13 14 15 15 14 Example 2 4 13 15 15 15 15 15 Example 3 5 14 15 15 15 15 15 Example 4 5 15 15 15 15 15 15 Comparative Example 1 10 9 8 13 11 11 10 Comparative Example 2 9 8 7 10 10 9 7 Comparative Example 3 13 10 9 12 12 10 9 Comparative Example 4 12 10 9 10 9 7 8 Comparative Example 5 10 8 7 10 8 7 5 Comparative Example 6 10 8 7 10 8 7 5 Comparative Example 7 10 9 9 10 9 8 5

[0108] The e-liquids prepared in Examples 1-4 have a gradually intensifying "smoky flavor," a delicate taste, minimal impurities, low irritation, and a subtle, natural freshness in the aftertaste, delivering a delightful floral and fruity aroma. While enjoying the floral and fruity notes, the smoky aroma is delicate and ethereal, resulting in a rich and lingering flavor. In contrast, the e-liquids prepared in Comparative Examples 1-7 lack flavor, have a shorter "smoky flavor retention time," and offer a poorer overall experience.

[0109] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An electronic e-liquid with a refreshing snail flavor, characterized in that: The electronic e-liquid comprises the following raw materials in parts by weight: 20-30 parts by weight of a refreshing extract, 1-5 parts by weight of a tobacco flavoring, 10-15 parts by weight of a tobacco leaf extract, 5-10 parts by weight of a snail-flavored flavoring, 10-15 parts by weight of a medicinal flavoring agent, 5-10 parts by weight of agar, 30-50 parts by weight of an organic solvent, and 5-15 parts by weight of olive oil. The snail-flavored essence includes snail extract, ginger extract, perilla extract, sugarcane juice, red chili extract, mushroom powder, and cardamom extract; The refreshing extracts include five-finger peach extract, lemon extract, and aloe vera extract; The medicinal ingredients include medicinal plant extracts, tangerine peel extract, and honeysuckle granules, wherein the medicinal plants are dandelion and *Clematis chinensis*.

2. The electronic e-liquid with a refreshing snail flavor according to claim 1, characterized in that: The preparation method of the snail extract includes the following steps: Step 1: Add the dried snail meat, chili powder, and Sichuan peppercorn powder to boiling water and simmer, stirring constantly, for 30-40 minutes. Then filter to obtain snail liquid and set aside. Step 2: Add the obtained snail liquid to water and continue to boil at a high temperature of 100-150℃. Add a thickener and boil until it becomes viscous. Add olive oil and concentrate at a low temperature of 50-60℃ for 2-3 hours. Dry to obtain snail extract.

3. The electronic e-liquid with a refreshing snail flavor according to claim 1, characterized in that: The preparation method of the Five-Finger Peach Extract includes the following steps: The roots of *Ficus hirta* were cut, cleaned, dried, and crushed. Then, a 50%-90% ethanol solution was added for extraction for 0.5-2 hours. After extraction, the solvent was removed to obtain a crude extract of *Ficus hirta*. The crude extract was then subjected to Soxhlet extraction with a 20%-90% ethanol solution. The extract was collected and concentrated under reduced pressure to obtain the *Ficus hirta* extract.

4. The electronic e-liquid with a refreshing snail flavor according to claim 1, characterized in that: The preparation method of the medicinal plant extract includes the following steps: (1) The picked medicinal plants are washed clean, dried and chopped, then soaked in water, refluxed and boiled for 2-4 hours, filtered, cooled and centrifuged, filtered, and the supernatant is freeze-dried to obtain the medicinal plant powder for later use. (2) Weigh the obtained medicinal plant powder and add it to ammonium acetate buffer solution. Stir to dissolve and then load it onto a cellulose chromatography column. Use different concentrations and different pH of ammonium acetate buffer solution to perform gradient elution in sequence to separate crude active substances. Continue to use phosphate buffer solution for elution and collect the active substances of medicinal plants. (3) Soak the active substances of the medicinal plant in a hot ethanol solution and stir to extract for 1-3 hours. After extraction, concentrate under reduced pressure to obtain the medicinal plant extract.

5. The electronic e-liquid with a refreshing snail flavor according to claim 1, characterized in that: The tobacco extract is a Zimbabwean tobacco extract.

6. The electronic e-liquid with a refreshing snail flavor according to claim 1, characterized in that: The tobacco flavoring includes at least one of ethyl acetate, isoamyl acetate, benzoic acid, phenylacetic acid, citric acid, limonene, and myrcene.

7. The electronic e-liquid with a refreshing snail flavor according to claim 1, characterized in that: The organic solvent includes at least one of glycerol, propylene glycol, and ethanol.

8. A method for preparing an electronic e-liquid with a refreshing snail flavor as described in any one of claims 1-7, characterized in that: The preparation method of the electronic e-liquid includes the following steps: By weight, the fresh extract and flavoring agent are dissolved in olive oil and heated at 80-100℃ for 10-15 minutes. Then, tobacco flavoring, tobacco leaf extract and agar are added and stirred evenly. The mixture is then heated to 120-150℃ and refluxed for 10-20 minutes. Next, the snail flavoring is dissolved in an organic solvent and added to the reflux reaction while stirring. After the addition is complete, the mixture is heated to 180-200℃ and refluxed for 10-20 minutes. The refluxed product is dissolved in 10-50 mL of organic solvent and filtered to obtain the electronic cigarette liquid.