Cordyceps militaris Za001 and its application

Abstract

The present invention relates to the field of microbial technology, and in particular to a Cordyceps militaris Za001 and its application. The present invention provides a Cordyceps militaris Za001, whose Latin name is Cordyceps militaris; it has been deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration on April 23, 2023, with a deposit number of CGMCC NO.40580. The Cordyceps militaris Za001 provided by the present invention has a high bioconversion rate of up to 150%, while the conversion rate of traditional Cordyceps militaris is only 120%, and the yield of the strain of the present invention is stable and not easily infected by miscellaneous pathogens.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to Cordyceps militaris Za001 and applications thereof. Background Art

[0002] Cordyceps militaris, also known as Cordyceps sinensis, is a precious medicinal fungus. It is rich in protein and amino acids, and contains more than 30 kinds of trace elements necessary for the human body. It is an excellent tonic. Modern medical research has shown that Cordyceps militaris contains nutrients such as cordycepin, cordycepic acid and various amino acids, which have the effects of nourishing the lungs and kidneys, stopping bleeding and reducing phlegm, dilating the trachea, calming, resisting various bacteria, and lowering blood pressure.

[0003] Although Cordyceps militaris has high nutritional and medicinal value, it is difficult to cultivate. After the research of scientists in recent years, Cordyceps militaris has finally been successfully cultivated artificially. The cultivation steps are to use high-quality rice or wheat as a culture medium, steam and sterilize, and inoculate Cordyceps sinensis. For example, CN 201611057512.7 discloses a Cordyceps militaris culture medium and a processing method thereof, which are prepared from the following raw materials: rice, wheat, corn, wheat bran, silkworm pupa powder, peptone, yeast powder, sucrose. The Cordyceps militaris cultured in the Cordyceps militaris culture medium prepared by it has the advantages of good quality, high yield, low cost, sufficient raw materials, good color, uniformity, and especially high content of amino acids and cordycepin. The success of artificial cultivation has promoted the development of functional foods.

[0004] However, the prior art still has defects such as high cultivation cost and low conversion rate, which result in that Cordyceps militaris and functional products prepared therefrom cannot be mass-produced and are sold at high prices. Therefore, how to develop more functional Cordyceps militaris with higher bioconversion rates is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0005] The purpose of the present invention is to provide a Cordyceps militaris Za001 and application thereof.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a Cordyceps militaris Za001, whose Latin name is Cordyceps militaris; it has been deposited in the General Microbiological Center of China Microorganism Culture Collection Administration on April 23, 2023, with a deposit number of CGMCCNO.40580.

[0008] The present invention also provides application of the Cordyceps militaris Za001 in producing Cordyceps militaris.

[0009] Compared with the prior art, the present invention has the following beneficial effects:

[0010] The Cordyceps militaris Za001 provided by the present invention has a high bioconversion rate of up to 150%, while the conversion rate of traditional Cordyceps militaris is only 120%. In addition, the yield of the strain of the present invention is stable and it is not easy to be infected by miscellaneous pathogens.

[0011] Biological Deposit Description

[0012] Cordyceps militaris Za001, Latin name: Cordyceps militaris;

[0013] This strain was deposited at the General Microbiology Center of China Culture Collection Administration on April 23, 2023, at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCCNO.40580. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0015] Figure 1 This is a state diagram of Cordyceps militaris Za001 of the present invention after being cultured for 25 days. DETAILED DESCRIPTION

[0016] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0017] Example 1

[0018] Cordyceps militaris Za001 was inoculated into PDA slant medium and cultured at 33°C for 7 days under light. The spores were washed with sterile water to make a spore count of 10 6 Take the prepared spore suspension and inoculate it into the seed culture medium at a 6% inoculation rate, and culture it in a shake flask at 33°C and 240rpm for 3 days to obtain the mother strain;

[0019] The seed culture medium is: water as solvent including: 42 g / L maltose, 8 g / L yeast extract, 0.6 g / L magnesium sulfate, and pH is 7.0.

[0020] Example 2

[0021] (1) Grind the oats to 35 mesh to obtain oat granules, mix the oat granules with 3 times the mass of water, and add KH 2 PO 4 , 0.5 g / L MgSO 4, 7g / L of vitamin B 1 , 5g / L of vitamin B 2 , and then sterilized at 121°C for 30 min to obtain the fermentation medium.

[0022] (2) The fermentation medium was placed in a culture bottle, and the mother strain obtained in Example 1 was inoculated into the fermentation medium at a 6% inoculum amount, and the culture bottle was placed in a culture room for cultivation;

[0023] Specifically, the culture medium was first cultured in the dark at 20°C for 4 days until the surface of the culture medium was completely covered with Cordyceps militaris mycelium; after the dark culture was completed, 550 Lux of light was applied, and light culture was carried out at 26°C, and the culture medium was harvested on the 50th day after inoculation.

[0024] Example 3

[0025] (1) Grind the oats to 35 mesh to obtain oat granules, mix the oat granules with 3 times the mass of water, and add KH 2 PO 4 , 0.6 g / L MgSO 4 , 6g / L of vitamin B 1 , 5g / L of vitamin B 2 , and then sterilized at 121°C for 30 min to obtain the fermentation medium.

[0026] (2) The fermentation medium was placed in a culture bottle, and the mother strain obtained in Example 1 was inoculated into the fermentation medium at a 6% inoculum amount, and the culture bottle was placed in a culture room for cultivation;

[0027] Specifically, the culture medium was first cultured in the dark at 21°C for 5 days until the surface of the culture medium was completely covered with Cordyceps militaris mycelium; after the dark culture was completed, 600 Lux of light was applied, and light culture was carried out at 26°C, and the culture medium was harvested on the 50th day after inoculation.

[0028] Example 4

[0029] (1) Grind the oats to 30 mesh to obtain oat granules, mix the oat granules with 3 times the mass of water, and add KH 2 PO 4 , 0.4 g / L MgSO 4 , 7g / L of vitamin B 1 , 5g / L of vitamin B 2 , and then sterilized at 121°C for 30 min to obtain the fermentation medium.

[0030] (2) The fermentation medium was placed in a culture bottle, and the mother strain obtained in Example 1 was inoculated into the fermentation medium at a 6% inoculum amount, and the culture bottle was placed in a culture room for cultivation;

[0031] Specifically, the culture medium was first cultured in the dark at 20°C for 4 days until the surface of the culture medium was completely covered with Cordyceps militaris mycelium; after the dark culture was completed, 550 Lux of light was applied, and light culture was carried out at 25°C, and the culture medium was harvested on the 52nd day after inoculation.

[0032] Comparative Example 1

[0033] (1) Grind the oats to 35 mesh to obtain oat granules, mix the oat granules with 3 times the mass of water, and add KH 2 PO 4 , 0.5 g / L MgSO 4 , 7g / L of vitamin B 1 , 5g / L of vitamin B 2 , and then sterilized at 121°C for 30 min to obtain the fermentation medium.

[0034] (2) The fermentation medium is placed in a culture bottle, and the mother strain of Cordyceps militaris (from Cordyceps militaris strain CGMCC No. 13179) is inoculated into the fermentation medium at a rate of 6%, and the fermentation medium is placed in a culture room for cultivation;

[0035] Specifically, the culture medium was first cultured in the dark at 20°C for 4 days until the surface of the culture medium was completely covered with Cordyceps militaris mycelium; after the dark culture was completed, 550 Lux of light was applied, and light culture was carried out at 26°C, and the culture medium was harvested on the 50th day after inoculation.

[0036] Experimental Example 1

[0037] The fresh Cordyceps militaris fruiting bodies harvested from Examples 2 to 4 and Comparative Example 1 were weighed, and the bioconversion rate of Cordyceps militaris was calculated. Two replicates were set for each test group, and the results were averaged, as shown in Table 1.

[0038] The calculation formula of bioconversion rate is:

[0039] Bioconversion rate = (fresh weight of Cordyceps militaris fruiting body / dry weight of oatmeal particles) × 100%

[0040] Table 1 Bioconversion rate

[0041] Group Bioconversion rate Example 2 157.4％ Example 3 153.6％ Example 4 151.3％ Comparative Example 1 124.6％

[0042] As shown in Table 1, the bioconversion rates of the strains provided by the present invention all exceeded 150%, while the bioconversion rate of conventional Cordyceps militaris strains was only 124.6%, which indicates that the strains provided by the present invention have higher production efficiency.

[0043] Experimental Example 2

[0044] The cordycepin content in the culture medium after harvest in Examples 2 to 4 and Comparative Example 1 was measured three times, and the average value was taken. The results are shown in Table 2.

[0045] Table 2 Cordycepin content

[0046] Group Cordycepin content Example 2 26.6mg / g Example 3 24.2mg / g Example 4 21.5mg / g Comparative Example 1 17.3mg / g

[0047] As shown in Table 2, the strain provided by the present invention has a good culture effect, can significantly increase the content of cordycepin in the culture medium, and is superior to the culture effect of the strain in the prior art.

[0048] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Cordyceps militaris Za001, whose Latin name is Cordyceps militaris; it was deposited in the General Microbiological Center of China Microorganism Culture Collection Administration on April 23, 2023, with the deposit number CGMCC NO.40580.

2. An application of the Cordyceps militaris Za001 according to claim 1 in producing Cordyceps militaris.

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