A composition containing fat-soluble vitamins

Abstract

The application discloses a composition containing fat-soluble vitamins, and is characterized in that the composition comprises the following components: a poorly soluble calcium salt, fat-soluble vitamins, vegetable oil, an emulsifier and water; the mass percentage of the poorly soluble calcium salt in the composition is 12-35%; the solubility of the poorly soluble calcium salt is less than 1 g / 100 mL, and the particle size is 800-2000 mesh; the fat-soluble vitamins comprise at least one of vitamin D, vitamin K, vitamin A and vitamin E, and the mass percentage of the fat-soluble vitamins in the composition is 0.00002-1.5%. The oil-in-water structure formed by the fat-soluble vitamins in the composition is more stable, the fat-soluble vitamins are more stable in the liquid preparation and are not easy to degrade, and the fat-soluble vitamins have a better effect. Meanwhile, the composition can promote the cell uptake rate of calcium, promote the absorption of calcium and improve the nutritional value of the composition.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of nutrient supplements, and particularly relates to a composition containing fat-soluble vitamins. BACKGROUND

[0002] In the food industry, it is difficult to directly add fat-soluble vitamins to foods or beverages with water as a base, so fat-soluble vitamins are usually dispersed in water by using emulsifiers to form oil-in-water emulsions, which are further spray-dried to form microcapsule powder as needed.

[0003] Although the formation of oil-in-water emulsions of fat-soluble vitamins enables them to be uniformly dissolved in water, the oil-in-water structure is not very stable, and the oil-in-water structure can be broken during preparation or long-term storage, causing the fat-soluble vitamins to be exposed, which makes the fat-soluble vitamins unstable in water and prone to degradation, resulting in reduced or lost effects of the fat-soluble vitamins; if metal ions are present in the system, the metal ions have a more significant effect on the stability of the vitamins, and the metal ions can act as catalysts to accelerate the oxidative degradation of the vitamins.

[0004] Currently, in order to improve the stability of fat-soluble vitamins in water or liquid preparations, other additives such as antioxidants are usually added to slow down the oxidation and invalidation rates of fat-soluble vitamins, but this method cannot make the oil-in-water structure of fat-soluble vitamins more stable, and still causes the exposure of fat-soluble vitamins, making the stability of fat-soluble vitamins in water still poor and prone to degradation, only the degradation rate is relatively slow. For example, patent document 1 (CN109645237A) discloses a liquid complex vitamin preparation with improved stability and a preparation method thereof, which comprises complex vitamins, BHT, mercaptoacetic acid, 2-mercaptoethanol, propyl gallate, OP-10, hydrogenated castor oil, sodium citrate, kesson, glycerol, and coconut oil. By carefully selecting and scientifically proportioning the types and contents of vitamin monomer protectants, stable storage of each vitamin monomer is achieved, and the liquid complex vitamin preparation has comprehensive nutritional effects, and the average weight gain rate of broiler chickens is significantly increased after taking it.

[0005] Therefore, how to improve the stability of the oil-in-water structure formed by fat-soluble vitamins is the key to improving the stability of fat-soluble vitamins and achieving better effects. SUMMARY

[0006] To solve the problems and deficiencies in the prior art, the present application provides a composition containing fat-soluble vitamins, which has a more stable oil-in-water structure formed by fat-soluble vitamins, making the fat-soluble vitamins more stable in liquid preparations and less prone to degradation, and having better effects. At the same time, the composition can also promote the cellular uptake rate of calcium, promote the absorption of calcium, and improve the nutritional value of the composition.

[0007] The present application provides a composition containing fat-soluble vitamins, comprising the following components: a poorly soluble calcium salt, a fat-soluble vitamin, a vegetable oil, an emulsifier, water; the mass fraction of the poorly soluble calcium salt in the composition is 12-35%; the solubility of the poorly soluble calcium salt is less than 1 g / 100 mL, and the particle size is 800-2000 mesh; the fat-soluble vitamin includes at least one of vitamin D, vitamin K, vitamin A, and vitamin E, and the mass fraction of the fat-soluble vitamin in the composition is 0.00002-1.5%.

[0008] However, fat-soluble vitamins are difficult to dissolve in water, so their application in solid preparations, liquid preparations and other dosage forms is limited. At present, they are mostly emulsified into emulsions or further spray-dried into microcapsule powder for application in the above dosage forms. The oil-in-water structure formed by fat-soluble vitamins is easily broken in water, exposing the fat-soluble vitamins and causing them to be unstable in water and easily degraded. Fat-soluble vitamins are also easily degraded after being emulsified, and their stability in water is even worse.

[0009] However, by introducing a poorly soluble calcium salt with a specific particle size and using it in combination with fat-soluble vitamins, and controlling the mass fraction of fat-soluble vitamins in the composition within a specific range, the stability of fat-soluble vitamins in liquid preparations can be improved. Specifically, the addition of a poorly soluble calcium salt with a specific particle size is beneficial to balancing the interaction force between the water phase and the fat-soluble oil phase droplets when they collide at high speed, and is beneficial to maintaining the mixed system containing the oil-in-water structure in a relatively stable state, thereby improving the stability of fat-soluble vitamins. If the particle size is too large, the collision force between the poorly soluble calcium salt and the fat-soluble oil phase droplets will be too large, the oil-in-water structure formed by the fat-soluble vitamins will be easily broken, the fat-soluble vitamins will be dissolved and degraded in water, which is not conducive to the stability of fat-soluble vitamins, and further not conducive to the subsequent effect of the composition. If the particle size is too small, the solubility of the calcium salt will increase, and the excessively dissolved calcium salt will ionize more calcium ions, and the more calcium ions will cause more damage to the fat-soluble vitamins, which is not conducive to the stability of fat-soluble vitamins. Moreover, a too small particle size will lead to more ionization of calcium ions and instability of vitamins.

[0010] Secondly, in the present application, the poorly soluble calcium salt refers to a calcium salt with a solubility of less than 1 g / 100 mL. The selection of a calcium salt with a specific solubility and content is more conducive to the formation of a stable mixed system of the poorly soluble calcium salt and the fat-soluble vitamins, promoting the stability of the oil-in-water structure in the mixed system and improving the stability of fat-soluble vitamins in liquid preparations. However, a calcium salt with high solubility will cause instability of fat-soluble vitamins due to excessive dissolution of the calcium salt.

[0011] In addition, the composition provided by the present application does not need to add any antioxidant, is safer and more stable, and has a better cell calcium uptake rate than commercially available liquid calcium-containing preparations, can effectively promote the absorption of calcium, and has a higher nutritional value.

[0012] Preferably, the fat-soluble vitamin includes vitamin K, and the mass fraction of vitamin K in the composition is 0.0001-0.001%.

[0013] Preferably, the fat-soluble vitamin includes vitamin D, and the mass fraction of vitamin D in the composition is 0.00002-0.00015%.

[0014] Preferably, the fat-soluble vitamin includes vitamin A, and the mass fraction of vitamin A in the composition is 0.0005-0.012%.

[0015] Preferably, the fat-soluble vitamin includes vitamin E, and the mass fraction of vitamin E in the composition is 0.015-1.5%.

[0016] Preferably, the poorly soluble calcium salt includes at least one of calcium citrate, calcium hydrogen phosphate, calcium dihydrogen phosphate, tricalcium phosphate, calcium sulfate, and calcium malate citrate.

[0017] Preferably, the mass fraction of the plant oil in the composition is 0.5-2.5%.

[0018] Preferably, the plant oil includes at least one of soybean oil, rapeseed oil, olive oil, walnut oil, peanut oil, sunflower seed oil, and corn oil.

[0019] Preferably, the mass fraction of the emulsifier in the composition is 0.5-2.5%.

[0020] Preferably, the emulsifier includes at least one of gum arabic and sodium starch octenyl succinate.

[0021] Preferably, the composition further includes a thickening agent, and / or a filler, and / or an acidity regulator.

[0022] Preferably, the mass fraction of the thickening agent in the composition is 0.2-0.5%.

[0023] Preferably, the thickening agent includes at least one of xanthan gum, pectin, guar gum, sodium carboxymethyl cellulose, and povidone K30.

[0024] Preferably, the mass fraction of the filler in the composition is 20-25%.

[0025] Preferably, the filler comprises at least one of xylitol, erythritol, D-mannitol, sorbitol, maltitol, isomalt, maltodextrin, dextrin, galactooligosaccharide, isomaltulose.

[0026] Preferably, the acidity regulator accounts for 0.3-1% by mass in the composition.

[0027] Preferably, the acidity regulator comprises at least one of citric acid, malic acid, tartaric acid, lactic acid, fumaric acid.

[0028] Preferably, the composition containing fat-soluble vitamins is prepared according to the following steps: S1. uniformly mixing fat-soluble vitamins and vegetable oil, heating at 60-85°C for 8-15 min to obtain an oil phase; S2. uniformly mixing an emulsifier and water to obtain an aqueous phase; S3. adding the oil phase to the aqueous phase and stirring at a speed of 6000-12000 rpm for 2-7 min to obtain an emulsion; S4. adding a poorly soluble calcium salt to the emulsion and stirring at a speed of 3000-8000 rpm for 3-8 min to obtain the composition. The composition liquid preparation prepared by the above method can stably exist in the liquid preparation for a long time, slow down the degradation of vitamins, and improve the effective nutritional value of vitamins.

[0029] Preferably, in S4, an acidity regulator, and / or a thickening agent, and / or a filler are further added.

[0030] In summary, the composition containing fat-soluble vitamins provided by the present application can stably exist in the composition liquid preparation, can reduce the degradation of vitamins, and improve the retention rate and nutritional absorption value of vitamins. At the same time, the composition liquid preparation has a high cell calcium uptake rate, can effectively promote the absorption of calcium, and improve the nutritional value of the composition. DETAILED DESCRIPTION

[0031] In order for those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present application, not all.

[0032] Embodiment 1

[0033] The composition liquid preparation is prepared according to the following steps in this embodiment:

[0034] S1. uniformly mixing vitamin D, vitamin K, and vegetable oil, heating at 80°C for 10 min to obtain an oil phase;

[0035] S2. uniformly mixing an emulsifier and water to obtain an aqueous phase;

[0036] S3. The oil phase is added to the water phase, stirring at 6000-12000 rpm for 2-7 min to obtain an emulsion;

[0037] S4. The acidity regulator, insoluble calcium salt, thickening agent, and filler are added to the emulsion, stirring at 3000-8000 rpm for 3-8 min to obtain the composition.

[0038] The proportions of the materials used in this example (mass percentage) are as follows: calcium citrate 800 mesh 24%, vitamin K 0.001%, vitamin D 0.00015%, soybean oil 1%, gum arabic 1%, xanthan gum 0.3%, xylitol 7%, malt dextrin 13%, citric acid 0.4%, and purified water 53.29885%.

[0039] The composition liquid preparation prepared in this example was tested for vitamin D, vitamin K, vitamin A, and vitamin E content. Vitamin D, vitamin A, and vitamin E were tested in accordance with GB 5009.82-2016, National Food Safety Standard for Determination of Vitamins A, D, and E in Food.

[0040] The content of vitamin K was tested as follows (using vitamin K2 as an example):

[0041] (1) Preparation of standard solution

[0042] Precisely weigh 10 mg of vitamin K2 reference substance, add isopropyl alcohol to a 100 mL volumetric flask, ultrasonically dissolve, cool, dilute to the mark, shake well, and dilute the reference solution to a working solution with a concentration of 1 μg / mL.

[0043] (2) Preparation of sample solution

[0044] Take 50 g of unopened composition liquid preparation sample, shake vigorously up and down, pour into a beaker, and stir with a glass rod. Weigh about 6 g of the mixed composition liquid preparation sample into a 50 mL centrifuge tube, add 15 mL of anhydrous ethanol, vortex for 5 min, add 15 mL of petroleum ether (30-60°C), vortex mix for 5 min, then add 15 mL of purified water, mix well, and centrifuge at 5000 r / min for 5 min until completely layered. Transfer the upper layer solution to a 150 mL flask, and repeat the petroleum ether addition operation twice. Combine the petroleum ether liquids, rotary evaporate at 40°C until dry, precisely add 10 mL of isopropyl alcohol, ultrasonically dissolve for 2 min, and filter through a 0.45 μm membrane to obtain the sample solution. The sample solution was tested using an Agilent 1260 high-performance liquid chromatograph. Note: The operation process should be carried out in the dark.

[0045] 3) Result calculation

[0046] The content of vitamin K2 in the sample was calculated using the external standard method.

[0047] X = (A1x W0x V1x K) / (A0x V0x W1) x M x 1000 x 1000

[0048] In the formula, A1 is the peak area of vitamin K2 in the sample solution; A0 is the peak area of vitamin K2 in the control solution; W1 is the weight of the sample, mg; W0 is the weight of the control, mg; V1 is the volume of the sample solution, mL; V0 is the volume of the control solution, mL; K is the purity of the control; M is the amount of sample per bottle, g; and 1000 is the unit conversion coefficient.

[0049] The retention rates of vitamins D, K, A, and E were calculated using the following formula: retention rate = vitamin content after 10 days of high-temperature investigation at 60°C / theoretical vitamin content * 100%. When the retention rate is ≥80%, it is considered to meet the requirements. The vitamin retention rates in each treatment group in the following examples are calculated according to the method in this example.

[0050] Example 2

[0051] In this example, 9 treatment groups were set up to explore the effect of calcium salt type on the retention rate of vitamins in the composition, taking vitamins D and K as examples. The 9 treatment groups were designated as treatment group 1#, treatment group 2#, treatment group 3#, treatment group 4#, treatment group 5#, treatment group 6#, treatment group 7#, treatment group 8#, and treatment group 9#, and the specific material ratios of the compositions of the 9 treatment groups are shown in Table 1. The retention rates of vitamins D and K of the compositions of the 9 treatment groups were tested, and the vitamin retention test results of these treatment groups were compared with those of Example 1, and the specific results are also shown in Table 1.

[0052] Table 1 Vitamin retention test results of the compositions of each treatment group in Example 2 and Example 1

[0053]

[0054] Analyzing the data in Table 1, we can see that the calcium salt used in Example 1, processing groups 1#-5# is a poorly soluble calcium salt (water solubility <1 g / 100 ml), and the retention rate of vitamin D and vitamin K in the final formula is >80%; the calcium salt in processing groups 6#, 7# is a water-soluble calcium salt (water solubility >3 g / 100 ml), and the retention rate of vitamin D and vitamin K is low, and lower than processing group 9# without adding calcium salt. This may be because the stability of vitamin D and vitamin K in the formula is related to the water solubility of the calcium salt in the formula. The higher the water solubility of the calcium salt, the higher the solubility of the ionized calcium ions, and the more serious the damage to the vitamins. Therefore, the formula with poorly soluble calcium salt is stable for vitamin D and vitamin K, and the formula with water-soluble calcium salt is not stable for vitamin D and vitamin K. In addition, in processing group 8#, the poorly soluble calcium salt is replaced by microcrystalline cellulose. In the food or health product industry, microcrystalline cellulose is commonly used as a stabilizer to promote the stability of some substances in liquid. However, in this invention, we found that the retention rate of vitamin D and vitamin K in the formula using microcrystalline cellulose was also poor, indicating that microcrystalline cellulose can improve the stability of vitamin D and vitamin K in liquid formulations, but the effect is not obvious.

[0055] Example 3

[0056] In this example, 5 processing groups were set to explore the effect of calcium salt particle size on the retention rate of vitamins in the composition, taking vitamin D and vitamin K as examples. The 5 processing groups are respectively referred to as processing group 10#, processing group 11#, processing group 12#, processing group 13#, and processing group 14#, and the specific material ratios of the compositions of the 5 processing groups are shown in Table 2. The retention rates of vitamin D and vitamin K of the compositions of the 5 processing groups were tested, and the test results of the retention rates of vitamins of these processing groups were compared with Example 1, and the specific results are also shown in Table 2.

[0057] Table 2 Vitamin retention rate test results of the compositions of each processing group in Example 3 and Example 1

[0058]

[0059] Analyzing the data in Table 2, we can see that in Example 1, treatment groups 10# and 11#, the particle size of calcium citrate is in the range of 800-2000 mesh, the retention rate of vitamin D and vitamin K is >80%, and vitamin D and vitamin K can stably exist in the formula. In treatment groups 12# and 13#, the particle size of calcium citrate is 700 and 80 mesh, respectively, and the particle size of calcium salt is too large, resulting in a retention rate of vitamin D and vitamin K both less than 80%, and vitamin D and vitamin K cannot stably exist in the formula. In treatment group 14#, the particle size of calcium citrate is 2500 mesh, and the particle size of calcium salt is too small, resulting in a retention rate of vitamin D and vitamin K of 47.31% and 45.72%, respectively, and vitamin D and vitamin K also cannot stably exist in the formula. This may be that the stability of vitamin D and vitamin K in the formula is related to the particle size of calcium citrate in the formula, and the collision of coarse particle size calcium citrate with the vitamin emulsion is violent, which destroys the structure of the emulsion, and the solubility of calcium citrate with too fine particle size increases, and calcium ions are ionized to destroy the vitamins. Therefore, too large or too small particle size of calcium salt is not conducive to the stability of vitamin D and vitamin K in the formula, and the calcium salt needs to be controlled in a specific particle size range to effectively improve the stability of vitamin D and vitamin K in the formula.

[0060] Example 4

[0061] In this example, five treatment groups were set to explore the effect of calcium salt content on the retention rate of vitamins in the composition, taking vitamin D and vitamin K as examples, and the calcium salt used in this example was 800 mesh calcium citrate. The five treatment groups are denoted as treatment group 15#, treatment group 16#, treatment group 17#, treatment group 18#, and treatment group 19#, and the specific material ratios of the compositions of the five treatment groups are shown in Table 3. The retention rates of vitamin D and vitamin K of the compositions of the five treatment groups were tested, and the test results of the retention rates of the vitamins of these treatment groups were compared with Example 1, and the specific results are also shown in Table 3.

[0062] Table 3 Vitamin retention rate test results of the compositions of the treatment groups in Example 4 and Example 1

[0063]

[0064]

[0065] Analyzing the data in Table 3, we can see that in the formula of Example 1, the calcium citrate content of the treatment groups 15# and 16# ranges from 12% to 35%, and the retention rates of vitamin D and vitamin K are >80%; in the formula of the treatment groups 17# and 18#, the calcium citrate content ranges from 5% to 10% (less than 12%), and the retention rates of vitamin D and vitamin K are <80%. The calcium citrate content of the formula of the treatment group 19# is 38%, which exceeds 35%, resulting in too high viscosity of the mixed system and making it impossible to proceed with normal preparation. This shows that the stability of vitamin D and vitamin K in the formula is related to the content of calcium citrate. Within a certain range, the higher the content of calcium citrate, the thicker the system obtained, the less calcium ions ionized from calcium citrate, and the more stable vitamin D and vitamin K. When the calcium citrate content in the formula exceeds 35%, the viscosity of the system is too high, and the process is not feasible.

[0066] Example 5

[0067] This example sets up a total of 4 treatment groups to explore the effect of insoluble calcium salt on the stability of vitamin D, vitamin K, vitamin A, and vitamin E in the system. The calcium salt used in this example is 800-mesh calcium citrate. The four treatment groups are respectively denoted as treatment group 20#, treatment group 21#, treatment group 22#, and treatment group 23#, and the specific material ratios of the compositions of the four treatment groups are shown in Table 4. Moreover, the retention rates of vitamin D, vitamin K, vitamin A, and vitamin E of the compositions of the four treatment groups are tested, and the specific results are also shown in Table 4.

[0068] Table 4 Vitamin retention rate test results of the compositions of each treatment group in Example 5 and Example 1

[0069]

[0070]

[0071] Analyzing the data in Table 4, we can see that vitamin D, K, A, and E can all stably exist in the mixed system under the action of insoluble calcium citrate, and their retention rates are all >80%. It should be noted that these fat-soluble vitamins D, K, A, and E can all or most stably exist in the system containing insoluble calcium salt.

[0072] Example 6

[0073] This example sets up a total of 1 treatment group, denoted as treatment group 24#, and uses a certain brand of commercially available calcium iron zinc oral liquid. The calcium intake rates of this treatment group and Example 1 are compared, and the specific results are shown in Table 5.

[0074] Among them, the test method of calcium intake rate is as follows:

[0075] 1. Caco-2 cells were seeded in 96-well black polystyrene microplate at a concentration of 6*104 cells / well in EMEM complete medium with 20% serum and incubated at 37°C in a 5% incubator for 24 hours. The experiment was divided into control group (no sample treatment with probe treatment & no sample no probe treatment group) and experimental group (including sample and probe treatment group & sample treatment no probe treatment group).

[0076] 2. The experimental group cells were treated with sample solution with a calcium concentration of 200 μg / ml prepared with complete medium, incubated at 37°C in a 5% incubator for 2 hours, and the control group was incubated with complete medium.

[0077] 3. All cells were washed with 200 μL of sterile phosphate buffer (PBS). The sample and probe treatment group was added with 100 μL of 2.5 μM Fluo-3-AM probe, and the sample treatment no probe treatment group was added with 100 μL of PBS, for 1 hour.

[0078] 4. 100 μL of PBS was added, and the excess fluorescent dye was washed with PBS.

[0079] 5. The detection was performed by using a microplate reader, and the excitation wavelength of fluorescence detection was set at 485 nm, and the emission wavelength was set at 525 nm. With the increase of calcium ion in Caco-2 cells, this will be reflected in the fluorescence score, and compared with the cells without sample treatment as control.

[0080] 6. The calcium uptake percentage formula is as follows:

[0081] Calcium uptake (%) = [(Cpf-Cp)-(Cf-C)] / (Cf-C)]*100%

[0082] Wherein Cpf is the fluorescence score of calcium content in the sample containing Fluo-3-AM in the cells, Cp is the fluorescence score of calcium content in the sample without Fluo-3-AM in the cells, Cf is the fluorescence score of calcium content in the cells containing Fluo-3-AM, and C is the fluorescence score of calcium content in the cells without Fluo-3-AM.

[0083] Table 5 Cell absorption data of the treatment group in Example 6, the composition of Example 1

[0084]

[0085] Analyzing the data in Table 5, we can see that the composition provided by the present application (Example 1) has a better calcium uptake rate than a certain brand of calcium iron zinc oral liquid on the market. This shows that the liquid preparation of the composition of the present application can promote the uptake of calcium and has a higher nutritional conversion rate.

[0086] The above examples are only used to illustrate the technical solutions of the present application and not to limit the protection scope of the present application. Although the present application is described in detail with reference to the above examples, it should be understood by those skilled in the art that the technical solutions of the present application can be modified or replaced equivalently, and these modifications or replacements are within the protection scope of the present application.

Claims

1. A liquid preparation of a composition containing a fat-soluble vitamin, characterized by, The liquid preparation comprises the following components: a poorly soluble calcium salt, a fat-soluble vitamin, a vegetable oil, an emulsifier, and water. The poorly soluble calcium salt accounts for 12-35% of the mass of the liquid preparation; the poorly soluble calcium salt has a solubility of less than 1 g / 100 mL and a particle size of 800-2000 mesh. The fat-soluble vitamin comprises at least one of vitamin D, vitamin K, vitamin A, and vitamin E, and the fat-soluble vitamin accounts for 0.00002-1.5% of the mass of the liquid preparation. The poorly soluble calcium salt comprises at least one of calcium citrate, calcium hydrogen phosphate, tricalcium phosphate, calcium sulfate, and calcium malate citrate.

2. The liquid preparation of the composition containing fat-soluble vitamins according to claim 1, wherein: The fat-soluble vitamin comprises vitamin K, and the vitamin K accounts for 0.0001-0.001% of the mass of the liquid preparation.

3. The liquid preparation of the composition containing fat-soluble vitamins according to claim 1, wherein: The fat-soluble vitamin comprises vitamin D, and the vitamin D accounts for 0.00002-0.00015% of the mass of the liquid preparation.

4. The liquid preparation of the composition containing fat-soluble vitamins according to claim 1, wherein: The fat-soluble vitamin comprises vitamin A, and the vitamin A accounts for 0.0005-0.012% of the mass of the liquid preparation.

5. The liquid preparation of the composition containing fat-soluble vitamins according to claim 1, wherein: The fat-soluble vitamin comprises vitamin E, and the vitamin E accounts for 0.015-1.5% of the mass of the liquid preparation.

6. The liquid preparation of the composition containing fat-soluble vitamins according to claim 1, wherein: The vegetable oil accounts for 0.5-2.5% of the mass of the liquid preparation.

7. The liquid preparation of the composition containing fat-soluble vitamins according to claim 1, wherein: The emulsifier accounts for 0.5-2.5% of the mass of the liquid preparation.

8. The liquid preparation of the composition containing fat-soluble vitamins according to claim 1, wherein: The liquid preparation further comprises a thickening agent, and / or a bulking agent, and / or an acidity regulator. The thickening agent accounts for 0.2-0.5% of the mass of the liquid preparation. The bulking agent accounts for 20-25% of the mass of the liquid preparation. The acidity regulator accounts for 0.3-1% of the mass of the liquid preparation.

9. The liquid preparation of the composition containing fat-soluble vitamins according to claim 1, wherein The liquid preparation is prepared according to the following steps: S1. Mix the fat-soluble vitamin and the vegetable oil uniformly, heat at 60-85°C for 8-15 min to obtain an oil phase; S2. Mix the emulsifier and the water uniformly to obtain an aqueous phase; S3. Add the oil phase to the aqueous phase, stir at a speed of 6000-12000 rpm for 2-7 min to obtain an emulsion; S4. Add the poorly soluble calcium salt to the emulsion, stir at a speed of 3000-8000 rpm for 3-8 min to obtain the liquid preparation.

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